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  • 1
    Electronic Resource
    Electronic Resource
    Minisatellite DNA ‘fingerprints’ can distinguish Rubus cultivars and estimate their degree of relatedness (1991)
    Springer
    Euphytica 53 (1991), S. 107-114 
    Details
    ISSN: 1573-5060
    Keywords: Rubus species ; raspberry ; blackberry ; cultivar identification ; M13 probe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Minisatellite DNA from 13 Rubus cultivars, including five raspberries, two R. flagellaris derivatives, three R. ursinus derivatives, and the three raspberry-blackberry hybrids Boysen, Logan Thornless and Young, was hybridized with the M13 probe yielding cultivar-specific DNA fragment profiles. Estimates of similarity, assessed from the fragment profiles, showed a strong association with the degree of genetic relatedness among the cultivars. Boysen was shown to be closely related to Austin Thornless, Logan and Young, and is thus thought to be of similar origin to Young, that is Austin Thornless x Phenomenal. While genetic relatedness of Creston with Lloyd George was suggested, Lloyd George parentage of Creston cannot be confirmed from the data obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023790
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  • 2
    Electronic Resource
    Electronic Resource
    DNA “fingerprints” detect genetic variation inAcer negundo (Aceraceae) (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 49-56 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Aceraceae ; Acer negundo ; box elder ; RFLPs ; minisatellite DNA ; M13 probe ; DNA analysis ; genetic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic DNA samples from 21 box elder plants collected in Missouri (U.S.A.) were digested with restriction enzyme and southern blot hybridized with the M13 minisatellite probe. Each plant was found to have a unique DNA fragment pattern. Moreover, levels of genetic variation estimated from a similarity index appear to be related to sampling distances. However, size of the fragments utilized in the analysis affects the estimates of genetic variation to a considerable degree.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937762
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  • 3
    Electronic Resource
    Electronic Resource
    The tetrapod “DNA fingerprinting” M13 repeat probe reveals genetic diversity and clonal growth in quaking aspen (Populus tremuloides, Salicaceae) (1991)
    Springer
    Plant systematics and evolution 175 (1991), S. 115-123 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Salicaceae ; Populus tremuloides ; Alpine community ecology ; clonal organisms ; DNA fingerprinting ; population genetics ; rflp analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A recently developed class of DNA endonuclease fragment probes (variously termed “minisatellite,” “DNA fingerprinting,” or “variable number tandem repeat loci” probes) has detected extensive intraspecific genetic variation in tetrapods. Here we use one probe from this class, the M 13 repeat probe (shown previously to yield ”DNA fingerprints” in humans) to examine genetic diversity in the quaking aspen. Comparisons of endonuclease fragment profiles of individuals separated by at least 6km reveals that diversification of alleles in this species has occurred to such an extent that the likelihood of two randomly chosen individuals having indistinguishableHaeIII fragment profiles is c. 3.17 × 10−4. Based on this finding, members of interdigitating clones can be assigned to one or another clone with high statistical confidence. Interdigitating, morphologically cryptic clones were also identifiable. These results demonstrate that some minisatellite probes can be applied to very distant taxa to obtain useful information about genetic variation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937841
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  • 4
    Electronic Resource
    Electronic Resource
    Pollen viability assessments in blackberries (Rubus subgen.Rubus) (1985)
    Springer
    Plant systematics and evolution 150 (1985), S. 281-290 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rosaceae ; Rubus ; Pollen viability ; pollen germination ; seed set ; cotton blue ; TTC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pollen viability has been investigated in 20 blackberry species using 3 methods, (1) cotton blue, (2) TTC, and (3) germination in a sucrose solution. Significant differences were found between species. Correlations between high pollen viability and high seed set were also obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984202
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  • 5
    Electronic Resource
    Electronic Resource
    Chromosome numbers and reproduction inRubus subgen.Malachobatus (1986)
    Springer
    Plant systematics and evolution 152 (1986), S. 211-218 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rosaceae ; Malachobatus ; Rubus ; Taxonomy ; crossing experiments ; apomixis ; chromosome numbers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A review of current knowledge of chromosome numbers and modes of reproduction in the genusRubus L. is presented. Chromosome numbers from some species of subg.Malachobatus Focke together with results of crossing experiments are reported for the first time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00989428
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  • 6
    Electronic Resource
    Electronic Resource
    Apomixis versus sexuality in blackberries (Rubus subgen.Rubus, Rosaceae) (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 207-218 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rosaceae ; Rubus ; Apomixis ; pseudogamy ; crossing experiments ; meiotic disturbances ; pollen fertility ; seed set
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract InRubus L. a connection seems to exist between the degree of meiotic disturbances on the one hand, and the production of unreduced embryo sacs, pollen fertility and relative seed set on the other hand. Severe meiotic disturbances commonly encountered in apomictic taxa decrease pollen fertility and thereby seed set since pollen is necessary for endosperm development. By contrast interspecific hybrids between apomictic taxa appear to be sexual and exhibit high pollen fertilities, probably due to an improved meiosis. Thus, apomixis leads to a decreased fertility inRubus, not the opposite, as often discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936048
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  • 7
    Electronic Resource
    Electronic Resource
    DNA fingerprint variation in some blackberry species (Rubus subg.Rubus, Rosaceae) (1996)
    Springer
    Plant systematics and evolution 199 (1996), S. 93-108 
    Details
    ISSN: 1615-6110
    Keywords: Rosaceae ; Rubus ; Taxonomy ; microspecies ; apomixis ; DNA analysis ; M13 probe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have analysed samples from Sweden, Denmark, and Germany of six facultatively apomictic blackberry species to investigate the accordance between a taxonomy based on morphological characters on the one hand, and distribution of genetic variation estimated by DNA fingerprinting on the other hand. DNA fingerprint variation was found to be quite restricted in all species investigated. The first taxonomic group included three species related toRubus nessensis, two being characterized by one very widespread DNA fingerprint in all three countries and a few rare ones, whereas the third species differed between Sweden and Germany. The second taxonomic group included species related toR. gracilis. Two of these species exhibited very similar DNA fingerprints, whereas the third species deviated clearly. The utilization of DNA fingerprinting as a tool in taxonomy is discussed; most likely this method could become a useful complement to morphology, especially in plant groups with reduced levels of genetic recombination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985920
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  • 8
    Electronic Resource
    Electronic Resource
    Different levels of morphometric variation in three heterogamous dogrose species (Rosa sect.Caninae, Rosaceae) (1997)
    Springer
    Plant systematics and evolution 204 (1997), S. 207-224 
    Details
    ISSN: 1615-6110
    Keywords: Rosaceae ; Rosa sect.Caninae ; Systematics ; taxonomy ; genetic variation ; hemisexual ; apomixis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Difficulties in delimiting well-defined entities in the dogroses (Rosa sect.Caninae) has resulted in very variable taxonomic treatments. The present study was undertaken to provide a background for taxonomy as well as plant breeding. Morphometric diversity was analysed on seedlings obtained from field collections in South Sweden of three species,Rosa dumalis, R. rubiginosa andR. villosa. A canonical variates analysis showed that the three species are relatively distinct whereas two subspecies ofR. dumalis were less well discriminated. Analyses of variance demonstrated that intraspecific variation is pronounced inR. dumalis and, to a lesser extent, inR. villosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00989206
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic diversity inChaenomeles (Rosaceae) revealed by RAPD analysis (1999)
    Springer
    Plant systematics and evolution 214 (1999), S. 131-145 
    Details
    ISSN: 1615-6110
    Keywords: Rosaceae ; Chaenomeles ; Taxonomy ; plant breeding ; DNA ; molecular marker ; quince
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic relatedness inChaenomeles was studied by RAPD analysis in 42 plants representing accessions of three wild species and one hybrid taxon. Amplification with 17 primers yielded a total of 156 polymorphic RAPD bands. Estimates of genetic relatedness suggest thatC. cathayensis andC. japonica are the most distantly related species, and that the former is comparatively homogeneous.Chaenomeles speciosa, which may have arisen through hybridization betweenC. cathayensis andC. japonica, takes an intermediate position between these two species. Analysis of diagnostic bands demonstrate that neitherC. speciosa norC. ×superba has any bands that do not occur in at least one ofC. cathayensis orC. japonica. Moreover,C. speciosa and the partly overlapping taxonC. ×superba are comparatively heterogeneous, which is also in accordance with a hybrid origin. Intraspecific variation was studied mainly inC. japonica; plants obtained from different sources of material formed well separated groups in the cluster analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985735
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  • 10
    Electronic Resource
    Electronic Resource
    Application of DNA fingerprinting to the taxonomy of European blackberry species (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1731-1735 
    Details
    ISSN: 0173-0835
    Keywords: Apomixis ; Parthenogenesis ; Taxonomy ; M13 bacteriophage ; Rubus ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Thousands of blackberry species (Rubus subgenus Rubus) have been described from Central and Northern Europe. These species are usually polyploid and pseudogamous. Since apomixis is only facultative, the species have retained the possibility to produce some offspring by sexual recombination. Hybridization of restriction-enzyme-digested DNA samples to the M13, (AC)/(TG) and 33.15 probes has yielded valuable information on the occurrence and distribution of genetic variation in Rubus. Intraspecific variation proved to be very restricted, with identical DNA fingerprints sometimes being exhibited by Swedish, Danish and German populations of the same species. By comparison, interspecific variation was usually pronounced, suggesting that most species have been derived through genetic recombination. Speciation through interspecific hybridization was demonstrated in one case. Several critical taxa were investigated; some of these proved to have unique DNA fingerprints, whereas others appear to be identical with previously described species. Evaluation of raspberry cultivars, obtained through several generations of vegetative propagation or with various in vitro techniques, failed to detect any DNA fingerprint variation, suggesting that the fingerprints are somatically stable. Blackberry seedlings, obtained by pollinating various species as well as interspecific hybrids with pollen from yet other species, were successfully evaluated with DNA fingerprinting to determine the relative proportions of the apomictic and sexual seed set.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601286
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