ALBERT

Description: Several cultivars of dwarf tomatoes and dwarf peppers were studied as possible candidate for space crops. Results showed the tomato cvs. Red Robin, Mohamed, and Sweet 'N' Neat produced the greatest yields, while pepper cvs. Pompeii, Red Skin, and Fruit Basket produced the greatest yields. The tomato and pepper cultivars were also analyzed by taste panels for organoleptic attributes, and all the cultivars were found to be acceptable by the taste panelists.

Description: NASA is currently developing technologies for use in the field of in-situ resource utilization (ISRU). One of the technologies being advanced is the Sabatier, or methanation, reactor which converts carbon dioxide and hydrogen into methane gas and water at high temperatures. This paper discusses the catalyst life and performance issues for these reactors that would be expected on Mars and describes the test methods employed and observed results. The various catalysts were tested in their capacity for the continuous production of methane gas via the Sabatier reaction and the possible effects of launch vibration loads, exposure to liquid water, particulate contamination, and chemical contamination to the overall observed reaction efficacy of the catalysts evaluated.

Description: The analysis of microscale to mm-scale astromaterials often involves the transfer of samples from storage or collection substrates to analytical substrates. These transfers are accomplished by hand (via tweezers or fine-tipped needles) or by utilizing micromanipulation instruments. Freehand manipulation of small particles is extremely challenging due to involuntary hand tremors on the order of 100m and due to the triboelectric charging induced by frequent contact between the manipulation tool and the support substrate. Months or years of practice may be required before an investigator develops the necessary experience to confidently transfer a 10-20m particle in this manner. Handling even mm-sized particles with fine-tipped tweezers can be challenging, due to the inability to precisely control the force with which grains are being held. Mechanical, hydraulic, and motorized/electrical micromanipulators enable the precise handling of microscale samples and are often utilized in laboratories where frequent small sample preparation is required. However, the price of such instruments (~ $10,000 to $100,000) makes them cost-prohibitive for some institutions. Graduate students or early-career scientists interested in conducting research on interplanetary dust particles, Itokawa particles returned by Hayabusa, or future samples returned by OSIRIS-REx or Hayabusa2 may experience difficulty in justifying the expense of a micromanipulator to their advisors or principle investigators. Johnson Space Centers Astromaterials Acquisition and Curation Office and the Lunar and Planetary Institute conduct annual training for early career scientists and for investigators that require experience with handling of small extraterrestrial samples. In support of this training, we have been developing low-cost mechanical alternatives to expensive micromanipulators that training participants can implement in their respective facilities.

Description: Surfaces of airless planetary bodies are exposed to micrometeorite bombardment and solar wind irradiation which alter the microstructural, compositional, and optical properties of regoliths over time. These processes are collectively known as space weathering, and they complicate the interpretation of remote sensing data and the subsequent characterization of airless surfaces. Within the next 5 years, NASAs OSIRIS-REx (Origins, Spectral Interpretation, Resource Identification, Security, Regolith Explorer) and JAXA (Japan Aerospace Exploration Agency)s Hayabusa2 missions will return samples from C-type asteroids Bennu and Ryugu, respectively. Compared to the Moon and S-type asteroids, our understanding of the space weathering of C-complex asteroids is limited. In order to maximize scientific return from remote sensing data and to prepare for the analysis of returned samples from these missions, we must better understand the effects of space weathering on hydrated, organic-rich materials. We can do so by simulating these processes in the laboratory. In this study, we simulate solar wind exposure through ion irradiation of the CM2 carbonaceous chondrite Murchison - a suitable analog for C-complex asteroids. Here, we present coordinated analyses of a sample before and after ion irradiation.

Description: NASAs Gateway will provide the capability for sustaining a human presence in cis-lunar space. Operations of the Gateway will include spacecraft dockings, extra vehicular activities (EVA), and high-power solar arrays. NASAs experience with the International Space Station highlighted the importance of evaluating spacecraft charging effects for such operations. For crewed spacecraft, which tend to employ the use of dielectric surfaces in this dynamic plasma environment, reliance on spacecraft charging simulation packages, such as the NASA/Air Force Spacecraft Charging Analyzer Program (Nascap-2k) [Mandell et al., 2006] and Spacecraft Plasma Interaction System (SPIS) [Roussel et al., 2008], is required to understand the risks to hardware and humans. The variability in the lunar plasma environment as the Moon revolves around the Earth, lunar wake effects, and a strong dependency on photoemission and secondary electron emission creates challenges for spacecraft charging analysis. The Design Specification for Natural Environments (DSNE) [NASA, MSFC] is the primary resource for space environments affecting NASAs crewed missions, and the DSNE provides plasma environments in a standard form for input into simulation packages. NASA developed the existing lunar plasma environment using data from Geotail [Nishida, 1994] along with published lunar plasma wake models [Halekas et al., 2005] based on Lunar Prospector. Since 2011, NASAs twin Acceleration Reconnection Turbulence & Electrodynamics of Moons Interaction with the Sun (ARTEMIS) satellites [Angelopoulos, 2010] have been collecting high resolution plasma and fields observations within the lunar plasma environment providing a much larger dataset of the plasma properties in cislunar space. This research compares the existing lunar plasma environment definition with ARTEMIS data and makes recommendations on the refinement of the environment definition for future lunar missions.

Description: No abstract available

Description: Planetary probes have long been a tool used by scientists to gain early clues on environments and systems of new planetary targets. This not only fueled the scientific process but also helped prepare for future missions, such as landers, and helped ensure their success. Venus, Earth's sister planet, has been the target of more probes and landers than any other body in our solar system except for Earth and yet many fundamental questions are unanswered. Challenge for Venus Surface Science: This lack of knowledge is a result of the challenging Venus environments. Remote sensing of the surface and portions of the atmosphere is difficult at best due to the thick layers of sulfuric acid clouds and the high pressure supercritical CO2 atmosphere below those clouds. This has hampered the ability of orbiting missions to provide us insight into surface features and processes and thus hides potential clues on interior process from our view. Surface probes and landers face an even more daunting challenge, which is the extreme temperature, pressure and unfriendly chemical composition of the near surface atmosphere. Over 10 assets have landed on the surface yet the longest surviving asset Venera-13 lasted only 127 minutes before succumbing to the extreme temperature. While this and other landers provided valuable new data, the short life impacted ability to understand any temporal processes, for example meteorology, seismic active, and therefore very little is known about the interior and surface atmosphere interactions. New Capability Offering Potential Solutions: Re-cent developments by NASA are offering hope of overcoming the technical challenges of surface operations and life with the use of high temperature electronics and systems. Wide band gap, SiC based electronics have been demonstrated to function successfully for extended periods of time both at 500C, Venus surface temperatures as well as when temperature is combined with the reactive chemistry of the surface atmosphere and the high pressures (over 90 bar pressure at the surface). In addition to electronics a number of other subsystems are in development including power in the form of high temperature batteries and power management devices, communications including antennas, transmitters and other components, materials, and structures and mechanisms. These are all activities under NASA's Long Lived In situ Solar System Exploration (LLISSE) project. Other activities are also funded under NASA's HOTTech program.

Description: Perform development to TRL 5/6 through ground demonstration in relevant environment. Perform component/subscale subsystem flight demonstrations on small/mid-size landers. Assess and characterize water in volatiles in lunar polar shadowed regions and craters. Reduce risk of ISRU for mission critical consumables through Integrated End-to-End Flight Demonstrations (pilot scale). Establish initial Human Mission Scale production capability to promote sustainable operations and as anchor for commercial involvement. Identify and characterize polar region environment and resources/volatiles for Science and future Exploration/Commercial applications. Provide ground-truth physical, mineral, and water/volatile resource characteristic information at multiple locations to provide geological context for science-focused theories of volatile placement and initial mining assessments.Test technologies and processes to reduce risk of future extraction/mining systems. Quantify concentration and lateral/vertical distribution of resources/volatiles. Utilize ISRU capabilities to Extend and Enhance Human Lunar Exploration Missions. Provide oxygen (and fuel) to enable reusable human lunar lander (10+ MT/yr O2)Process carbon-based crew waste/trash into gases and propellants; can reduce logistics while minimizing public perception issues (alternative is conversion to radiation shielding). Scavenge unused propellants and hardware from spent landers. Metal extraction from regolith as feedstock for in situ and in space manufacturing demonstrations. Civil engineering and construction aimed at future outpost/infrastructure build-up. Develop and Demonstrate ISRU for Human Mars Missions. ISRU for propellant production (10-15 MT/yr); Liquefy, store, transfer, and refuel ascent vehicle. Use Moon for operational experience and mission validation for Mars: Pre-deployment & remote activation and operation without crew. Storing and transferring mission consumables Landing crew with empty tanks with ISRU propellants already made and waiting. Support/Promote Commercialization of Space. Large scale polar ice mining (100+ MT/yr water)O2/H2 propulsion for landers/cis-lunar transportation with surface and in space depots. In situ construction and energy expansion at mining and human outpost site(s). ISRU Ground Development. Develop and advance ISRU technologies to enable acquisition of resources and processing into mission consumables. Utilize Multi-center collaboration with a portfolio that includes internal NASA work, external contracts, and collaborative agreements/partnerships. Where appropriate, develop lunar ISRU components and subsystems with a Mars-forward application. Engage industry through public-private partnerships to lay the foundation for long-term lunar and space economic development. Spin-in/spin-out technologies for terrestrial applications and industry (mining, oil & gas, alternative energy, construction). Flight Demonstration Path to Operational ISRU. Utilize small demonstrations with near off-the-shelf hardware to obtain critical information quickly on lunar resources and operations. Demonstrate critical technologies and processes that interact with lunar materials and environments. Perform 'pilot plant' demonstrations at architecture relevant scales and durations to reduce the risk for ISRU-provided products for critical human mission applications.

Description: We will investigate the use of galactic cosmic ray (GCR) secondary particles to probe the deep interiors of small solar system bodies (SSBs), including comets, asteroids, and geologic structures on the surfaces of airless bodies. Applications include solar system science, planetary defense, and resource utilization. Our Phase I study demonstrated that muons, the long-range charged component of GCR showers, can penetrate SSBs up to a km in diameter, providing information on their interior structure. Muons produced in Earths atmosphere have been applied to image the interior of large objects for science and engineering. In Phase I, we found that the production of muons in the solid surfaces of airless bodies is much smaller than in Earths atmosphere. Nevertheless, the flux of transmitted muons is sufficient to detect inclusions within an asteroid or comet in a reasonable amount of time, ranging from hours to weeks, depending on the size of the SSB and the density contrast, position and size of the inclusion. For asteroids and comets, large density variations (e.g., porous soil or ice versus solid rock) are relatively easy to detect. The intrinsic spatial resolution of muon radiography (muography) is on the scale of a few meters. The spatial resolution that can be achieved in practice depends on signal intensity and integration time (counting statistics), the angular resolution of the muon tracker (hodoscope) and details of data reduction and analysis methodology. Our Phase II project will assess remaining unknowns for the application of muography to determining the interior structure of SSBs, assess risks for implementation, and provide a roadmap for development of SSB muography beyond the NIAC program. To achieve our objectives, we will focus on four interrelated tasks: Task1) Signal and background characterization: Characterize the production and transmission of muons and secondary particle backgrounds made by cosmic ray showers in SSBs; and near-surface features from radiographic and tomographic data; Task2) Imaging studies: Develop methods to determine the density structure of SSB interiors and near-surface features from radiographic and tomographic data; Task3) Instrument design: Using simulations and bench-top laboratory experiments, investigate specific concepts for the design of compact hodoscopes and components; Task4) Synthesis: Combine the results of the first three tasks to determine the range of applicability of the method, identify the steps needed for maturation of the concept, and explore concepts for a pilot muography mission.

Description: Liquid water was abundant on early Mars, but whether the climate was warm and wet or cold and icy with punctuated periods of melting is still poorly understood. Modern climate models for Mars tend to predict a colder, icier early climate than previously imagined [e.g., 1]. However, any model for the early climate on Mars must be reconciled with the chemical record. We currently do not understand how alteration mineralogy formed in snow and ice dominated conditions compares to that of warmer climates, and it is unclear whether cold climate weathering could form all or any of the aqueous alteration phases expressed on early martian surfaces [2]. To help resolve this knowledge gap, we synthesize results from glacial Mars analog sites at the Three Sisters, Oregon and mafic regions of the Antarctic ice sheet, and compare them to the surface mineralogy of Mars. These sites provide the opportunity to investigate weathering in environments analogous to glacial environments on Mars throughout geologic time, including snowpacks or smaller wet-based or polythermal glaciers [3, 4] as well as the proposed extensive ice sheets of the late Noachian icy highlands model

Description: In late 2017, the laser intensity monitor (LIM) current began to decline on the Lunar Reconnaissance Orbiter (LRO) miniature inertial measurement unit (MIMU). The MIMU was powered off in March 2018 and has only been used during extended eclipses, a pre-eclipse orbit phasing maneuver, and critical momentum unloads. Science slews were suspended, and the onboard extended Kalman filter (EKF) was disabled. A coarse rate was estimated through star tracker quaternion differentiation, and attitude was provided directly from a single star tracker. A complementary filter, combining the differentiated quaternions with the integrated acceleration derived from the attitude control torque, was developed, tested, and uploaded to the spacecraft in December 2018. The EKF has been enabled, using the complementary filter rate in place of the MIMU and science slews are now being performed. This paper presents an overview of the complementary filter rate estimation and EKF changes, fault detection updates without the MIMU, and inflight performance improvements.

Description: The Martian atmosphere, consisting mainly of gaseous CO2, is regarded as one of the main planetary resources capable of providing a significant portion of the oxygen that will be needed for human missions to the planet's surface. NASA's In-Situ Re-source Utilization (ISRU) project supports the development of oxygen generation technologies that can convert the Martian atmosphere into usable oxygen. The thin CO2 based Martian atmosphere, however, carries certain levels of dust stirred up by the Martian winds that must be filtered out at the front end of any CO2 acquisition system. Thus, the ISRU project is developing particulate filters as part of a Mars CO2 acquisition sys-tem. A prototype filter system, known as the Scroll Filter, is being tested under simulated Martian atmospheric conditions in the Mar Atmospheric Flow Loop at the NASA GRC. The measurement techniques as well as the preliminary results from a series of performance tests will be discussed.

Description: Supplemental safe food production has been an essential goal of NASA to meet the nutritional needs of astronauts on the International Space Station (ISS) as well as for future long duration missions to the moon and beyond. Food crops grown in space experience different environmental conditions than plants grown on Earth (i.e. microgravity and spaceflight physical sciences impacts). To test the growth methods and effects of the space environment, red romaine lettuce Lactuca sativa cv. 'Outredgeous', was grown in Veggie plant growth chambers on the ISS. Microbiological food safety of the plants grown on the ISS was determined by heterotrophic plate counts to assess total microbial load for bacteria and fungi as well as screening for specific pathogens and isolate identification. Molecular characterization was completed using Next Generation Sequencing (NGS) to provide valuable information on the taxonomic composition and community structure of the plant microbiome. Chemical analyses of plant tissue were conducted to understand spaceflight-induced changes in key elements in the space diet, phenolics, anthocyanin levels, and Oxygen radical absorbance capacity (ORAC), a measure of antioxidant capacity. Three growth tests of red romaine lettuce were completed on ISS, VEG-01A, VEG-01B, and VEG-03A. Plants were harvested using two harvest methods, either a single terminal harvest (after 33 days) or cut-and-come-again repetitive harvesting (64 days total growth). Ground controls were grown simultaneously with a delay to accommodate condition monitoring and replication. A comparison of the plant tissue returned to Earth showed leaves from the second grow-out had significantly higher bacterial counts than the preceding or subsequent growth test or any of the ground controls. Fungal counts were significantly higher on the final cut-and-come-again harvest of the third grow out. None of the potential foodborne pathogens that were screened for were detected. Bacterial and fungal isolate identification and community characterization indicated similar diversity between VEG-01A and VEG-01B growth tests, however, there appeared to be subtle differences in diversity and distribution among the three growth tests. Chemical analysis of plant tissue revealed significant variation in a few elemental data, but variation in levels of phenolics, anthocyanins, and ORAC was not significantly different. This study indicated that leafy vegetable crops could safely provide an edible supplement to astronauts' diet, and our analysis provided baseline data for continual operation of the Veggie plant growth units on ISS. This research was funded by NASA's space biology program.

Description: The vestibulospinal system provides the spinal motor circuits controlling head/neck and limb movements and body posture with rapid reflex adjustments to maintain equilibrium and stability and with a continuous essential excitatory drive, called tonus, to enhance reactive responses to perturbations that force the animal off normal posture. The sensory signals to these reflex circuits originate from hair cells in the inner ear of otolith structures, namely the utricle and saccule, that transduce inertial acceleration and orientation of the head with respect to gravity and in the three orthogonally arranged semicircular canals that transduce angular head rotation. The principal vestibulospinal pathways are 1) the medial vestibulospinal tract that descends in the ventromedial funiculus and innervates inter- and motoneurons located mainly in lamina VII, VIII, and dorsomedial IX throughout the cervical segments; and 2) the lateral vestibulospinal tracts that course in the lateral to ventrolateral funiculi and are distinguished by two divisions: i) a cervical-projecting tract that overlaps many of the targets of medial vestibulospinal tract neurons including the motoneurons in ventromedial IX and also contributes to reflex control of shoulder and forelimb (arm) muscles; and ii) a lumbosacral-projecting tract that provides a rapid input to maintain stable posture and reflex control of the lower body. A striking observation in understanding the functional organization of this sensory-motor system is both that the driving sensory input can be dynamically modified by the behavioral context in which the sensation is made and that it remains able to quickly respond to an external force during self-generated head movements. The structural basis for vestibulospinal inputs to spinal motor control circuits in quadrupeds and bipeds rely in part on the animal's need for coordination between fore- and hind-limb reflex movements. Understanding the sensory-to-motor transformations in the diverse species rely on the correlations of the conserved and unique species behavior, morphology and physiologic function.

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Description: The ability to predict cancer risk associated with exposure to low doses of high-LET ionizing radiation (IR) remains a challenge. Epidemiological methods lack the sensitivity and power to provide detailed risk estimates for cancer and ignore individual variance in IR sensitivity. We have hypothesized that DNA repair capacity can be used as a marker to evaluate and differentiate individual radiation sensitivity. More specifically, this work is based on the concept that the combined time-dose dependence of radiation-induced foci (RIF) of p53-binding protein 1 (53BP1) following low-LET exposure contains sufficient information to infer sensitivity to any other LET. Our hypothesis was tested in 15 different mouse strains as well as in primary human immune cells. We first approached individual ionizing radiation sensitivity in a mouse model by culturing primary skin fibroblasts extracted from 76 mice of 15 different genetic backgrounds and exposing them to HZE particles and X-rays. This work is one of the most extensive studies on the kinetics and possible genetic underpinnings of radiation-induced DNA damage and repair. Our results is in agreement with a DNA repair model we previously postulated, where nearby DNA double strand breaks (DSB) in the nucleus are brought together for more efficient repair, leading to RIF clustering. Such mechanism was evidenced by a specific dose and LET dependence of RIF numbers. Briefly, RIF quantification after low-LET X-ray exposure showed an asymptotic saturation for doses between 1 Gy and 4 Gy 4 hours post-irradiation across all 15 strains. The clustering of DSB across all strains also led to more RIF/Gy for lower LET (X-ray and 350 MeV/n Ar) than for higher LET (600 MeV/n Fe) 4 hours post-exposure. Considering the fact that the number of DSB/Gy should be independent of LET, our data suggest there are more DSB in individual RIF as the LET increases. RIF numbers for 24 and 48 hours post-exposure led to the inverse trend, with more remaining RIF/Gy for higher LET (by 600 MeV/n Fe). This result suggests cells have more difficulty resolving RIF from higher LET as they the number DSB/RIF increases. Note that for most conditions, the variance of RIF/Gy was small within individual animals of the same strain and large between strains, suggesting a strong genetics component. Furthermore, we present our preliminary data from an ongoing study on human genetic associations with IR sensitivity. To address the human variability in responses to HZE particle irradiation in a maximally comprehensive manner, we are in the process of collecting and isolating primary blood mononuclear cells from 768 healthy subjects of European descent, 18-75 years of age, 50/50 male/female distribution. We have analyzed 53BP1+ RIF formation as well as oxidative stress and cell death in primary cells from 192 subjects in response to the same HZE particles as used in mice: 600 MeV/n Fe, 350 MeV/n Ar and 350 MeV/n Si, 1.1 and 3 particles/100m2, 4 and 24 hours after irradiation. We will next complete the quantification of HZE particle-induced DNA and cellular damage in the remaining subjects and compare it to their responses to low-LET irradiation. Finally, we will perform GWAS analysis to identify human genomic associations with IR sensitivity and potential targets for biomarker development.

Description: The Mars Science Laboratory Curiosity rover landed in Gale crater in August 2012 to investigate early Hesperian-aged sedimentary rocks on the lower slopes of Aeolis Mons (i.e., Mount Sharp) that show variations in phyllosilicates, hematite, and sulfates from orbital reflectance spectroscopy, suggesting changes in ancient aqueous environments. During the Eighth International Conference on Mars in July 2014, Curiosity was still traversing the Bradbury group on the plains of Gale crater (Aeolis Palus) and had only analyzed four samples in its internal laboratories. Soon after Mars 8, Curiosity began its investigation of Mount Sharp and has since driven through ~350 m of vertical stratigraphy, the majority of which is part of the Murray formation. The Murray fm is comprised primarily of laminated mudstone with occasional sandstone and heterolithic facies and represents a long-lived fluvio-lacustrine environment. Curiosity has analyzed 13 drilled rock samples from the Murray formation and 4 from the ancient eolian Stimson fm with the Chemistry and Mineralogy (CheMin) instrument. Here, we discuss the mineralogy of all fluvio-lacustrine samples analyzed to date and what these results tell us about sources of the sediments, aqueous environments, and habitability of ancient Gale crater.

Description: Temperature-swing adsorption pumps have been proposed as a method of acquiring and compressing Martian atmospheric CO2 for downstream processing. Most industrial applications and previous research targeted at space in-situ resource utilization (ISRU) utilize long (~hours) temperature swing periods, typically limited by the ability to transfer heat from a naturally insulating sorbent bed. A rapid cycle adsorption pump (RCAP) would reduce these periods to minutes, in the hope of increasing overall throughput. This paper details the design and preliminary experimental results from testing an RCAP in a simulated Martian environment. The test configuration features a central, liquid-cooled and heated heat transfer plate surrounded by symmetrical rectangular sorbent beds. Various bed thicknesses and commercially available Zeolite 13X sorbent particle sizes are evaluated to both determine performance and provide data for a parallel modeling effort. Discussions of multi-stage configurations and methods of boosting bed conductivity are included.

Description: The purpose of this NCRP commentary is to provide the current state of knowledge on the effects of ionizing radiation on the immune system and on latent herpes virus reactivation to the scientific community and government agencies. Its purpose is to better understand radiation-induced latent virus reactivation, which is possibly an underestimated consequence of ionizing radiation exposure. This activity should involve the radiation research community (academia, industry and regulatory agencies) and government agencies (NASA, DOD, CDC).

Description: We report here on a survey of a lava tube cave by a rover that is instrumented for astrobiology missions. The NASA Ames testbed rover, CaveR, was deployed in Valentine Cave in Lava Beds National Monument (N. CA, USA) during August of 2018. The rover carried an instrument package consisting of Near Infrared and Visible Spectrometer System (NIRVSS) a point spectrometer operating in 1590-3400 nm range, sensitive to H2O and -OH bearing minerals, pyroxenes, and carbonates (Roush, et al 2018); the bore sighted Drill Operations Camera (DOC), a monochrome imager illuminated by LEDs at 410, 540, 640, 740, 905 and 940 nm; a Realsense depth sensor system for 3D model generation; and a high resolution DSLR stereo camera. The payload was mounted on a tiltable instrument platform attached to the left side of the rover. The rover was driven manually in the cave by field operators, following instructions from a remote science operations team, and simulating a mission concept with science-guided autonomy. A simulated mission took place for 3 days with a team of 3 scientists selecting targets and interpreting data from the payload. To begin the mission, the rover drove along one wall of the cave imaging continuously with the Realsense in 20 m cave segments, three total. At the start of each day, the images from a 20m segment and a panorama stitched from them were provided to the science team to examine. The science team used these data to prioritize specific points along the cave wall for the collection of NIRVSS, DOC, and DSLR data. The objective of the data collection was to identify and study putative biological and mineralogical features in the cave. The data were delivered in xGDS, a customized mapping, planning, and data base management software developed at NASA Ames (Lee, et al 2013). Once the targets for further observations were selected, a plan for collecting the observations (positions in the cave and pointing for each requested observation) was constructed using xGDS and delivered to a rover team to execute the science data collection plan. Acquired data were delivered back to the science team for analysis. Preliminary results from the experiment illustrate the utility of the system (rover plus payload) to study the cave geology and mineralogy and its potential for identifying biomineral features.

Description: During the late summer, the author sailed to the Antarctic South Shetland Islands to survey the microorganisms living in marine (tidal pools) and freshwater (moss saturated with snow melt) environmental niches. Equipped with a microscope to take video of samples within hours of collection to capture a pristine condition, the authors found a dense and diverse ecology that included species with unique patterns of locomotion. Capturing the organism's movement expedited identification, but it also showed the dynamic way each organism's mobility fit together like a puzzle to create a complex ecosystem.

Description: This presentation will be an introduction and overview of space crop production needs, goals, and challenges in the areas of robotics and automation for the workshop Aug. 6-7, 2019 at Kennedy Space Center. This presentation will be used to start the workshop and set the direction.

Description: Future missions to the Sun-Earth Libration L1 and L2 regions will require scheduled servicing to maintain hardware and replenish consumables. While there have been statements made by various NASA programs regarding servicing of vehicles at these locations or in Cis-lunar space, a practical transfer study has not been extensively investigated in an operational fashion to determine the impacts of navigation and maneuver errors. This investigation uses dynamical systems and operational models to design transfer trajectories between the Sun-Earth Libration region (QuasiHalo orbit) and the Earth-Moon vicinity (Distant Retrograde Orbit, QuasiHalo Orbit, Halo Orbit, and Near Rectilinear Halo Orbit). We address the total V cost of transfers and operational considerations between each pair of locations using a Monte Carlo analysis.

Description: There are limited resources on extraterrestrial bodies and current launch vehicles can only send finite resources on deep space missions. Transporting resources needed for deep space missions is extremely expensive; at tens of thousands of dollars per pound launched. In order to sustain life and establish outposts on other celestial bodies, it is necessary to find a way to utilize the resources that are readily available in these environments. This process is called In Situ Resource Utilization (ISRU), which is comprised of searching for, collecting, processing, and storing materials found on other celestial bodies and using them for construction, science applications, propulsion, and other life sustaining purposes. The processing and use of regolith, which is a loose rocky material found on the surface of bodies like the Moon or Mars, is the main focus of ISRU work done in Swamp Works in the Engineering Development Lab at Kennedy Space Center (KSC). The research Swamp Works has done in regolith applications ranges from construction and infrastructure to propellant production to storage of oxygen and water.

Description: New surface damage models are presented that predict the heat shield erosion due to dust particle impact when a spacecraft enters the Martian atmosphere. Existing models were based on Apollo-era experimental data and approximate methods for tracking the dust particle trajectories through the shock layer. These legacy methods will be compared against new results based on more sophisticated particle tracking methods and recent experimental data.

Description: No abstract available

Description: No abstract available

Description: The Lunar Development Lab (LDL) is a new concept to bring together academia, industry, non-profit organizations and NASA in an accelerator environment to generate new design solutions, technologies and architectures that will lead to the first human lunar outpost. By leveraging key partnerships in lunar science, mining, construction, chemical engineering and other key fields as well as making available rapid design, economic analysis, artificial intelligence (AI) and machine learning (ML) tools, significant progress can be made in a short amount of time. Therefore, the goal of LDL is to accelerate development and focus on economic solutions that can lead to sustainable and economical human lunar outpost.

Description: Shergottites, the largest martian meteorite group, come from at least two geochemically different source reservoirs i.e. incompatible trace element (ITE)-depleted and enriched. The depleted shergottites are thought to be derived from an ITE-depleted mantle reservoir, while enriched shergottites are thought to be derived from an ITE-enriched mantle reservoir that represents late stage residual melt from a magma ocean or interaction with martian crust. Moreover, the martian crust is distinct from shergottites, by being highly oxidized, distinctly ITE-enriched, and older. The link between the crust and shergottite compositions is poorly understood. Here we model shergottite differentiation to resolve the origin of enriched shergottites and why the bulk martian crust is compositionally distinct from shergottites. Early formed olivine-hosted melt inclusions can provide primary melt composition from which the parental magma had crystallized and also information at different stages of crystallization during parent magma differentiation that leads to shergottite magma evolution as well as crustal contribution assessment. We analyzed olivine-hosted melt inclusions of two enriched poikilitic shergottites for their major, minor and trace element concentrations using electron microprobe and laser ablation ICP-MS. We corrected the melt inclusion compositions for post-entrapment re-equilibration with their host olivine. To comprehend the crystallization sequence of these rocks and whether the melt entrapment is consistent with the crystallization, we use MELTS models for equilibrium and fractional crystallization. The results of these models suggest that all the melts were trapped in a closed system progressive crystallization at 1150-1210 C within 1 kbar to 1 bar pressure that is equivalent to 〈8.5 km, implying melt entrapment without any additional exogenous materials.

Description: The ice giant planets, Uranus and Neptune, represent an important and relatively unexplored class of planet. Most of our detailed information about them comes from fleeting looks by the Voyager 2 spacecraft in the 1980s. Voyager, and ground-based work since then, found that these planets, their satellites, rings, and magnetospheres, challenge our understanding of the formation and evolution of planetary systems. We also now know that Uranus- Neptune size planets are common around other stars. These are some of the reasons ice giant exploration was a high priority in NASA's most recent Planetary Science Decadal Survey. In preparation for the next Decadal Survey, NASA, with ESA participation, conducted a broad study of possible ice giant missions in the 20242037 timeframe. This paper summarizes the key results of the study, and addresses questions that have been raised by the science community and in a recent NASA review. Foremost amongst these are questions about the science objectives, the science payload, and the importance of an atmospheric probe. The conclusions of the NASA/ESA study remain valid. In particular, it is a high priority to send an orbiter and atmospheric probe to at least one of the ice giants, with instrumentation to study all components of an ice giant system. Uranus and Neptune are found to be equally compelling as science targets. The two planets are not equivalent, however, and each system has things to teach us the other cannot. An additional mission study is needed to refine plans for future exploration of these worlds.

Description: No abstract available

Description: No abstract available

Description: The PERISCOPE study focuses primarily on lunar caves, due to the potential for being imaged in orbital scenarios. In the intervening years, from 2012-2015, scientists developed further rationales and interest in the scientific value of lunar caves. It does not appear that they are likely to be sinks for water-ice due to the relatively warm temperatures(~-20 degrees Celsius) in the caves leading to geologically-rapid migration of unbound water due to sublimation, and inevitable loss through any skylights. However, the skylights themselves reveal apparent complex layering, which may speak to a more complex multi-stage evolution of mare flood basalts than previously considered, and so their examination may provide even more insight into the lunar mare, which in turn provide a primary record of early solar system crustal formal and evolution processes. Further extrapolation of these insights can be found within the exoplanet community of researchers,who find the information useful for calibrating star formation and planetary evolution models. In addition, catalogues of lunar and martian skylights, "caves" or "atypical pit craters" have been developed, with numbers for both bodies now in the low hundreds thanks to additional high resolution surveys and revisiting the existing image databases.

Description: We have conducted a comprehensive study of propionitrile (C2H5CN) ice from the amorphous to crystalline phase in order to provide detailed information on this specific cyanide, which may potentially contribute to the chemical composition of the Haystack ice cloud observed in Titan's stratosphere by the Cassini Composite InfraRed Spectrometer (CIRS). Infrared transmission spectra of thin films of pure propionitrile ices deposited at low temperature (30-160 K) were collected from 50 cm1 to 11,700 cm1 (200-0.85 m). The far-infrared spectral region was specifically targeted to compare with CIRS far-infrared limb spectra. The temperature and time evolution of C2H5CN ice was thoroughly investigated to better understand discrepancies reported in pre- viously published laboratory studies on the crystalline phase of C2H5CN. Specifically, we observe peculiar temperature and time-driven ice phase transitions, revealed by significant spectral variations in the ice, which stabilizes once a complete crystalline phase is achieved. From these results, the crystalline phase of propionitrile ice was identified at deposition temperatures greater than or equal to 135 K and 〈 140 K. Our findings corro- borate previous studies that ruled out pure propionitrile ice as the sole chemical identity of Titan's observed Haystack emission feature. In order to understand and identify the Haystack cloud, we have initiated co-de- position experiments that incorporate mixtures of Titan-relevant organics, many of which have corresponding vapors that are abundantly present in Titan's stratosphere. In this paper, we present the result of one example of a co-deposited ternary ice mixture containing 16% hydrogen cyanide (HCN), 23% C2H5CN, and 61% benzene (C6H6). Although this co-condensed ice mixture is the best fit thus far obtained to match the broad width of the Haystack, it is still not the appropriate chemical candidate. However, it reveals an intriguing result: the strong lattice mode of pure C2H5CN ice is drastically altered by the surrounding molecules as a result of mixing in a co- condensed phase. The laboratory results reported here on propionitrile ice may help to further constrain the chemical identification of Titan's stratospheric Haystack ice cloud, as well as improve on the current state of knowledge of Titan's stratospheric ice cloud chemistry.

Description: Mapping the D/H isotopic ratio across Mars provides unique insights into the evolution and climatology of its atmosphere, and may help to identify the sources and sinks of atmospheric water vapor on the planet. We present new spatially-resolved measurements of atmospheric H2O, HDO and D/H on Mars during its northern summer at Ls = 126, on March 21, 2016. High-resolution spectra were acquired at /~40,000 using CSHELL, the Cryogenic Near-IR Facility Spectrograph at the 3 m NASA Infrared Telescope Facility (IRTF) on top of Maunakea, Hawaii. We targeted the 22 spectral band of H2O around 2990 cm1 (3.3 m), and its deuterated form HDO at its 1 fundamental band around 2720 cm1 (3.7 m). The water vapor and HDO show increased mixing ratios in the northern hemisphere, reaching peak values of 400 ppmv for H2O, 170 ppbv450 ppbv for HDO, as compared to the southern hemisphere where depleted values of 〈 20 ppmv for H2O and 〈 10 ppbv for HDO were observed. The resulting D/H measurements indicate an enrichment over the terrestrial value, exhibiting a strong variation with latitude, longitude and local times. We report a strong dependence of D/H on local time, with high HDO abundances towards local noon. We observed higher D/H enrichment above basins (Utopia), lower enrichment above high-altitude Mons (Elysium Mons), and low D/H variations over at regions on the planet.

Description: The Cassini mission performed 127 targeted ybys of Titan during its 13 yr mission to Saturn, culminating in the Grand Finale between 2017 April and September. Here we demonstrate the use of the Atacama Large Millimeter/ submillimeter Array (ALMA) to continue Cassini's legacy for chemical and climatological studies of Titan's atmosphere. Whole-hemisphere, interferometric spectral maps of HCN, HNC, HC3N, CH3CN, C2H3CN, C2H5CN,and C3H8 were obtained using ALMA in 2017 May at moderate (0 2, or 1300 km) spatial resolution, revealing the effects of seasonally variable chemistry and dynamics on the distribution of each species. The ALMA submillimeter observations of HCN and HC3N are consistent with Cassini infrared data on these species, obtained in the same month. Chemical/dynamical lifetimes of a few years are inferred for C2H3CN and C2H5CN, in reasonably close agreement with the latest chemical models incorporating the sticking of C2H5CN to stratospheric aerosol particles. ALMA radial limb ux proles provide column density information as a function of altitude, revealing maximum abundances in the thermosphere (above 600 km) for HCN, HNC, HC3N, and C2H5CN. This constitutes the rst detailed measurement of the spatial distribution of HNC, which is found to be conned predominantly to altitudes above 730 60 km. The HNC emission map shows an east-west hemisphericasymmetry of 13% 3%. These results are consistent with very rapid production (and loss) of HNC in Titan's uppermost atmosphere, making this molecule an effective probe of short-timescale (diurnal) ionospheric processes.

Description: Current NASA plans for lunar exploration include a human lunar landing system, comprised of separate descent andascent modules, with the eventual goal of reusability. Different oxygen production processes were studied to evaluatethe feasibility of producing 10 tons of oxygen per year assuming a high latitude landing location. The study includesconsideration of packaging the ISRU components on the descent module, methods to transfer the regolith from theexcavators to the processing plant which may be mounted well above the lunar surface, and general concept ofoperations for excavation, oxygen production, and liquefaction and storage. A solar-based power system was alsodesigned and packaged on the lander, including the use of direct solar thermal energy where appropriate.

Description: LEAVES (Lofted Environmental Atmospheric Venus Sensors) is a design exercise with the goal of dramatically decreasing the cost of obtaining prioritized chemical and physical data in planetary atmospheres. Through the application of a swarm approach this concept parallelizes atmospheric exploration, with geographic coverage far exceeding what is possible with conventional monolithic platforms or sondes. Each unit in the swarm is exceptionally compact, with a powered payload mass of only a few tens of grams and a high-drag, semi-rigid structure that acts to slow each probe as it descends through the atmosphere. This structural design can collapse into a planar form to allow for efficient stowage prior to arrival at the target body. With a total per-unit mass of only 120 g, a fleet of 100 (or more) units can be very reasonably accommodated on a carrier spacecraft.Science operations, which begin when the LEAVES probes reach an altitude of 100 km, are targeted for the cloud-bearing region of Venus' atmosphere. During the roughly 9 hour, terminal velocity descent through the atmosphere, LEAVES collects data of the state and composition of the atmosphere in parallel across multiple units. These data would represent an unprecedented constraint on the distribution and concentration of targeted chemical species, and the detection of local and regional variations in both chemistry and physical properties.A novel and compelling result of this exercise was that the same optimization that produced a structure with an exceptionally low areal mass density (0.126 kg/m2) also resulted in a probe that can be deployed directly from an aerobraking orbit (~140 km at 5 km/s) without the need for aeroshell protection. This translates to a tremendous mass savings and gives LEAVES the flexibility to be carried as a secondary payload aboard either a descending surface probe or an orbital radar mapper. Because such missions are under active development or have already been proposed (but not flown), we infer that LEAVES is well positioned as a technology

Description: Asteroids contain a wealth of resources including water and precious metals that can be extracted. These resources could be applied to in-space manufacture of products that depend less on material launched from Earth's surface. The Meteoroid Impact Detection for Exploration of Asteroids (MIDEA) concept addresses the challenge of characterizing an asteroid surface using a small satellite with a constellation of free-flying plasma sensors to assess the asteroids viability for in situ resource utilization (ISRU). The plasma sensors detect ions ejected from the surface of an asteroid by meteoroid impacts, enabling the surface composition to be inferred. The objective of this NIAC Phase I study was to demonstrate feasibility of the MIDEA architecture in the context of proximity operations around an asteroid target and to develop the design of an orbital geometry and attitude control strategy for the ultralight plasma sensors. This was undertaken through a simulation framework to identify and characterize a favorable orbit for the MIDEA sensor constellation, and developing a sensor geometry that is consistent with maintaining the pointing requirements necessary to operate with sufficient power generation. Our study showed that a polar orbit aligned along the asteroid terminator provided sufficient stability for the sensors in the low gravitational environment under the influence of substantial solar radiation pressure. Reflector vanes using controlled reflectivity devices implemented with liquid crystal technology are capable of maintaining the sensor attitude so that it consistently points its solar panels in the sun direction and the sensor electrode at the asteroid surface. Finally, the reduction in meteoroid impact detection due to visibility constraints from the proposed orbit does not substantially extend the expected mission duration. These results indicate that the MIDEA concept can be achievable using a 1020 kg spacecraft, which would be able to characterize the surface composition of an asteroid within 3050 days of proximity operations. This architecture, implemented in parallel to multiple asteroid targets, would enable widespread exploration of near-Earth asteroids at low cost.

Description: Because planetary missions to Mars take years from initial design to arrival at Mars, and because of the unpredictability of major global dust storms, the de-sign of the thermal protection system (TPS) of a Mars entry vehicle requires an estimation for the potential damage caused by dust particle impacts on the heat-shield. This paper will review previous analytical and experimental approaches to modeling dust particle ero-sion and will compare the legacy models against more modern computational techniques and new dust ero-sion models that will be based on upcoming experi-ments in the German Aerospace Center (DLR) GBK facility. The various models will be compared by incorporating them into the Icarus material response code applied to a representative vehicle entering the Martian atmosphere.

Description: We present retrievals of dust particle effective radius during the 2018 planet-encircling dust storm from the Mars Science Laboratory Curiosity rover. Four independent observations with three of the rover's instruments were used for retrievals. We find dust particle radii exceeded 4 m and possibly 5 m during the height of the storm, which represent the largest dust particles yet seen in the martian atmosphere.

Description: The current best estimates of Bennus gravity field will be presented, based on the independent solutions from four different teams involved on the OSIRIS-REx mission. The discovery of ejected particles about Bennu that may remain in orbit for several days or more provide a unique opportunity to probe the gravity field to higher degree and order than possible by using conventional spacecraft tracking. However, the non-gravitational forces acting on these particles must also be characterized, and their impact on solution accuracy must be assessed. This talk will present the latest results from the mission, incorporating spacecraft tracking from the lowest orbit in which the satellite will be during the mission.

Description: NASA GeneLab is an open-access repository for omics datasets generated by biological experiments conducted in space or ground experiments relevant to spaceflight (e.g. simulated cosmic radiation, simulated microgravity, bed rest studies). The GeneLab Data Systems (GLDS) version 4.0 will be available on October 1st 2019, and will provide a state-of-the-art bioinformatics platform for the space biology and radiation communities to upload their data into an omics data commons, to process their data with vetted standard workflows and to compare with existing analyses. Started in 2015 as a repository designed to archive omics data from space experiments, GeneLab has expanded its scope to all ionizing radiation omics experiments conducted on the ground and has put considerable effort in providing carefully characterized radiation metadata on all datasets. GeneLab is also providing processed data derived from the raw data covering a large spectrum of omics (genome, epigenome, transcriptome, epitranscriptome, proteome, metabolome) to help users explore important questions: 1) Which genes or proteins are expressed differently in space for various living organisms? 2) What specific DNA mutations or epigenetic changes happen in space or after exposure to ionizing radiation? and 3) How does genetics affect these responses? Processed data available on GeneLab are derived by standard data analysis workflows vetted by hundreds of scientists who volunteered to join one of the four GeneLab Analysis Working Groups (Animal AWG, Plant AWG, Microbe AWG, Multi-Omics AWG). In this presentation, we will discuss how to bridge the gap between irradiation studies performed on earth and biological experiments conducted in space since the early 1990's. We will discuss how radiation dosimetry was estimated for datasets derived from samples collected during the Space Shuttle era on the International Space Station and on other orbiting platforms. Finally, we will address future strategies regarding dose monitoring in future missions into space, inter-agency efforts to unify data under one umbrella, and knowledge dissemination across the radiation research community and the space biology community.

Description: Mars sample return is a bold concept, which entails gathering a varied, scientifically-relevant collection of Martian rock core samples and bringing them to Earth for analysis. To support this endeavor, the Marshall Space Flight Center (MSFC) is developing the Mars Ascent Vehicle (MAV), which is responsible for getting the collected samples off the planet. The MAV Preliminary Architecture Assessment (PAA) study is designing two vehicle architectures based on different propulsion configurations: a two-stage solid-solid concept, and a hybrid concept. Given different thrust profiles for the two configurations, each concept uses a unique trajectory to reach the same orbit. In support of the PAA, The MSFC Natural Environments Branch (EV44) was asked to produce tables of atmospheric parameters along each of the two trajectories. The Mars Global Reference Atmospheric Model (Mars-GRAM) is an EV44 tool that is ideally suited for this analysis. Mars-GRAM will continue supporting MAV development in future design cycles.

Description: We observed lunar exospheric potassium D1 (7,698.9646 ) emissions using a high-spectral resolution Fabry-Perot spectrometer in 2014. We present the first potassium line profile measurements, which are representative of the potassium velocity distribution. Inferred temperatures are greater during the waxing gibbous phase, 1920 630 K and lower at waning gibbous phase, 980 200 K. Exosphere models suggest that the measured line widths are a combination of photon-stimulated desorption and impact vaporization sources. The relative potassium emission intensity decreases by 2.5 between lunar phases 80 and 30 and is brightest off the northwest limb near the Aristarchus crater, which is a potassium-rich surface region. Additionally, the emissions off the northern limb are brighter than the southern limb. The intensity decrease and the greater line width during the waxing gibbous versus the waning gibbous phase suggests a dawn-dusk asymmetry.

Description: To enable return of human missions to the surface of the Moon sustainably, a new study was initiated to assess the feasibility of developing an evolvable, economical and sustainable lunar surface infrastructure using a public-private partnerships approach. This approach would establish partnerships between NASA and private industry to mutually develop lunar surface infrastructure capabilities to support robotic missions initially and later evolve to full-scale commercial infrastructure services in support of human missions. These infrastructure services may range from power systems, communication and navigation systems, thermal management systems, mobility systems, water and propellant production to life support systems for human habitats. The public-private partnerships approach for this study leverages best practices from NASAs Commercial Orbital Transportation Services (COTS) program which introduced an innovative and economical approach for partnering with industry to develop commercial cargo transportation services to the International Space Station (ISS). In this approach, NASA and industry partners shared cost and risk throughout the development phase which led to dramatic reduction in development and operations costs of these transportation services. Following this approach, a Lunar COTS concept was conceived to develop cost-effective surface infrastructure capabilities in partnership with industry to provide economical, operational services for small-scale robotic missions. As a result, a self-contained lunar infrastructure system with power, thermal, communication and navigation elements was conceptually designed to increase capability, extend mission duration and reduce cost of small-scale robotic missions. To support human missions, this work has now been extended to analyze full-scale lunar infrastructure systems. This infrastructure system should have capabilities to support human missions from a few days to several months with minimal maintenance and replacement of parts. This infrastructure system should also maximize the use of existing lunar resources, such as, oxygen from regolith, water from ice deposits at the poles, and use of metals, such as iron and aluminum, from lunar regolith. The plan includes a buildup of these capabilities using a phased-development approach that will eventually lead to operational infrastructure services. By partnering with industry to develop and operate the infrastructure services using the COTS model, this plan should also result in significant cost savings and increased reliability. This paper will describe the Lunar COTS concept goals, objectives and approach for developing an evolvable, economical and sustainable human lunar infrastructure as well as the challenges and opportunities for development.

Description: Visions and Voyages for Planetary Science in the Decade 2013-2022[1] identified the goal of understanding the origin, evolution, and processes that control climate on terrestrial planets,with direct interest in Venus. The Glenn Extreme Environment Rig (GEER), located at NASA Glenn Research Center, was developed to address a community need for a facility which could simulate the extreme environments of the Venus surface. It actively supports science investigations and technical development activities of research institutions and industry partners. It is uniquely suited for studying the interactions between Venus' substantial atmosphere, its surface, and exploration components. Ongoing facility enhancements will provide significant additional value to the research community and maintain GEERs status as a world-class Venus simulation facility.

Description: Molecular biosignatures are key targets for current, proposed, and future life detection missions. With the high accuracy and low limit of detection (LOD) that new and future instruments will require, decontamination of life detection hardware is necessary to prevent false positives. Lipids are a molecular biosignature of interest, as they are ubiquitous to all life as we know it, can survive unaltered in the geologic record for longer than any other biomolecule (i.e. billions of years), and form through both biotic and abiotic processes. Lipids display origin-diagnostic molecular patterns that can reveal biotic or abiotic synthesis, so finding them and ascertaining their molecular features is important for potentially detecting evidence of life elsewhere. Traditional methods of decontamination, or contamination control (CC), primarily clean hardware through fabrication in sterile (cleanroom) environments, killing microbes, and removing/flushing contaminants off instrument and spacecraft components. However, research suggests that some standard cleaning methods are either unlikely to remove lipid contaminants or are incompatible with life detection instrument materials. To solve this problem, I propose to find, test, and verify a decontamination method that thoroughly cleans instruments by destroying lipid molecules, but is simultaneously compatible with major materials used in these instruments. I will study the effects of traditional CC methods (including Dry Heat Microbial Reduction and Vapor phase Hydrogen Peroxide) and experimental CC methods (Electron Beam Irradiation) on lipid molecules for application to life detection instrumentation. I will then develop a CC plan for a novel lipid detector (ExCALiBR, Extractor for Chemical Analysis of Lipid Biomarkers in Regolith) searching for lipids in either soil or icy world scenarios. This plan will uphold planetary protection regulation requirements and validate experimental analyses of in-situ life detection tests.

Description: Slightly less than 50 years after the deployment of Apollo 11 seismometer, and slightly more than 41 years after the operational end of the combined Apollo seismic network, seismology is back to operations in planetary science. InSight, or Interior Exploration using Seismic Investigations, Geodesy and Heat Transport, is a mission dedicated to understand the formation and evolution of terrestrial planets through the investigation of the interior structure and processes of Mars. This presentation will outline early mission results, focusing primarily on SEIS, the Seismic Experiment for Interior Structure.

Description: The existence of mass-independently fractionated sulfur in Archean rocks is almost universally accepted as evidence for low atmospheric O2 and O3 concentrations at that time. But the detailed patterns of the values and of the ratios / and / remain to be explained, and the mechanism for producing the mass-independent fractionation remains controversial. Here, we explore the hypothesis that the relatively low values seen during the Mid-Archean, 2.7-3.5 Ga, were caused by the presence of organic haze produced from photolysis of methane. This haze helped shield SO2 from photolysis, while at the same time providing surfaces on which unfractionated short-chain sulfur species could condense. The evolution of oxygenic photosynthesis, and the concomitant disappearance of organic haze towards the end of the Archean allowed more negatively fractionated S4 and S8 to form, thereby generating large positive fractionations in other sulfur species, including sulfate and H2S. Reduction of this sulfate to H2S by bacteria, followed by incorporation of H2S into pyrite, produced the large positive values observed in the Neoarchean rock record, 2.5-2.7 Ga.

Description: It is a truism within the exoplanet field that to know the planet, you must know the star. This pertains to the physical properties of the star (i.e. mass, radius, luminosity, age, multiplicity), the activity and magnetic fields, as well as the stellar elemental abundances which can be used as a proxy for planetary composition. In this white paper, we discuss important stellar characteristics that require attention in upcoming ground- and space-based missions, such that their processes can be understood and either detangled from that of the planet, correlated with the presence of a planet, or utilized in lieu of direct planetary observations.

Description: Why Earth has an oxygen-rich atmosphere is not a solved problem, although the crucial importance of O2 to life on Earth, and its generation by life on Earth, are unquestioned. The factors that promote or frustrate the generation of free oxygen are central to what we mean by habitability, because it is O2 that makes a world fit for creatures like us attending conferences like these. The astronomical mission to identify and characterize an inhabited planet remains justifiably focused on the quest to detect O2 (or its byproduct O3) because free oxygen remains the bronze standard of habitation as we know it. We can expect that eventually, perhaps within 100 years, we will have accumulated a database of such exoplanets and we will begin to be able to evaluate basic hypotheses regarding the origin of oxygen (if not the origin of life).

Description: No abstract available

Description: The proposed Habitable Exoplanet (HabEx) astrophysics facility is one of four large such facilities being proposed to the 2020 decadal. It is a large telescope that is sensitive to ultraviolet, optical, and near-infrared photons. The proposed designs overall length is on the order of 17.2 m and its maximum cross section is on the order of 5.25 X 5.25 m. The primary mirror is 4 m in diameter. A transient dynamic analysis was performed to estimate the order of magnitude of ring down time after moving the telescope and pointing at a new target for science planning purposes. Without uncertainty factors, results from a simple re-pointing maneuver indicate that primary to secondary mirror LOS errors are on the order of 10-4 pico-m after 5 minutes. Also, a frequency response analysis was performed to predict the impact of planned micro-thruster vibrations on required stability. Based on provided noise level associated with the micro-thrusters and loading assumptions and without uncertainty factors, the assessed vibrations do not impact predicted performance requirements.

Description: The frequency of Earth-size planets in the habitable zone(HZ)of Sun-like stars, hereafter, is a key parameter to evaluate the yield of nearby Earth analogs that can be detected and characterized by future missions. Yet, this value is poorly constrained as there are no reliable exoplanet candidates in the HZ of Sun-like stars in the Kepler field. Here, we show that extrapolations relying on the population of small(〈1.8R), short-period(〈25 days)planets bias to large values. As the radius distribution at short orbital periods is strongly affected by atmospheric loss, we reevaluate using exoplanets at larger separations. We find that drops considerably, to values of only5% - 10%. Observations of young(〈100 Myr)clusters can probe short-period sub-Neptunes that still retain most of their envelope mass. As such, they can be used to quantify the contamination of sub-Neptunes to the population of Kepler short-period small planets and aid in more reliable estimates of.

Description: The many known health risks currently associated with space travel include increased risk of cardiovascular disease, cancer, central nervous system related diseases, muscle degeneration, and changes with host-gut microbiome interactions that can have profound impact with these and other health risks. The majority of the risk from space travel stem of the two components of the space environment which are microgravity and radiation. Two specific systemic effects have been uncovered by us to impact the body as a whole due to the space environment. One factor is related from our earlier work (Beheshti et al, PLOS One, 2018), we predicted that there is a systemic component of the host that causes general increased health risks due to spaceflight driven by a circulating microRNA (miRNA) signature consisting of 13 miRNAs that directly regulates both p53 and TGF1. MiRNAs are small non-coding RNA molecules with a negative and post-transcriptional regulation on gene expression) are increasingly recognized as major systemic regulators of responses to stressors, including microgravity, oxidative stress, and DNA damage. In addition, due to the size and stability of miRNAs, it is known that miRNAs can circulate throughout the body and have been found in the majority of the bodily fluids including blood, urine, saliva, and tears. Here, we start to dissect the actual impact of this miRNA signature on both the radiation and microgravity components and prove that this miRNA signature actually exists in the circulation of a host. The other systemic factor we uncovered was the impact the mitochondria on the whole body due to spaceflight. We hypothesize that spaceflight may promote a physiologic response driven by systemic mitochondria pathways leading to metabolic disorder stemming from the liver and directly impacting other organs and tissues. A systems biology method was implemented utilizing GeneLab datasets that involved in vitro experiments performed at the low Earth orbit, in vivo experiments involving mice flown to space, and finally human physiological data from astronauts. A comprehensive multi-omics approach was implemented which involved correlating transcriptomic analysis with proteomics, metabolomics, and methylation analysis. This approach led us to confirm our hypothesis that a systemic mitochondrial driven response is responsible for increasing potential health risk and is conserved from the in vitro studies, to the in vivo studies, and finally confirmed in astronauts.

Description: Space crop production will be important in future long duration exploration missions to supplement the packaged diet with fresh bioactive nutrients. Plant care and the addition of fresh veggies to the diet may also have a role in astronaut well-being. Pick-and-eat salad crops are the best candidates for this near-term supplementation since they require minimal processing or preparation to add to meals. While light quality can strongly influence plant responses on Earth, the impacts of light quality on plant growth and composition in spaceflight remain unclear. The VEG-04 experiment uses two Veggie plant growth chambers on the International Space Station to simultaneously test different red: blue light ratios on the growth of Mizuna mustard, a leafy green salad crop. In addition to plant health and yield, the composition of key nutrients is assessed. Astronauts conduct on-board organoleptic evaluation of the fresh produce. Microbial food safety of returned produce is examined, and a Hazard Analysis Critical Control Point (HACCP) plan has been developed for this crop. VEG-04 consists of two experiments, one lasting 28 days with a single harvest, and the second lasting 56 days, with three cut-and-come-again harvests. These different scenarios provide an opportunity to test two production concepts, examine different fertilizers, monitor microbial changes over time for this crop, and assess potential impacts of interacting with plants on crew behavioral health and performance in spaceflight operations. In ground testing, plant growth was not significantly different across the different light treatments, however nutrient composition did differ significantly. Flight test results will be compared with ground data. This research was co-funded by NASA's Human Research Program and Space Biology in the ILSRA 2015 NRA call.

Description: The impact of spaceflight on immune function is undoubtedly a critical focus in the area of space biology and human health research. Heat shock proteins (Hsp) are an evolutionarily conserved family of proteins that are expressed in response to cellular and physiological stressors, experienced during radiation exposure, confinement, circadian rhythm disruption, and altered gravity (hypergravity experienced at launch/landing and microgravity experienced in-flight). In particular, Hsp70 aids in the folding of proteins, facilitates the movement of proteins across the membranes during signal transductions and can stimulate innate immunity. Since Hsp70 is induced during cellular stress, and can act as a stimulator for innate immunity, we sought to address how a loss of Hsp70 affects immunity, under the stress-inducing model of acute and chronic hypergravity. Moreover, the effects of gravity as a continuum on the induction of Hsps and key immune genes were also assessed to determine if increased cellular stress, via increased gravity (g)-force, contributes to immune dysfunctions. For this, wildtype (W1118) and Hsp70 deficient (Hsp70null) Drosophila melanogaster were subjected to simulated hypergravity at increasing levels of g-force (1.2g, 3g, and 5g) for acute (1hr) and chronic (7-day) timepoints and were compared to 0g 'non-hypergravity' controls. Following simulation, whole bodies were sex-segregated, RNA was isolated and quantitative (q)PCR was performed to determine differential immune gene expression profiles. Further, functional output of hemocytes were assessed by a phagocytosis assay. Collectively, these studies evaluated the effects of Hsp70 in the context of immunity during acute and chronic hypergravity. Indeed, relevance for this work can directly translate to acute effects of launch/landing gravitational forces upon liftoff (~1.7g) and entry (~3.4g) that astronauts experience. In addition, the effects of chronic cellular stress is directly relevant to the immune health of astronauts on long duration missions, as well. Thus, as we approach the goal of returning to the Moon and landing the first humans on Mars, an evaluation of gravity as a continuum and the stress-inducing effects of altered gravity experienced during spaceflight on astronaut immunity and health are necessary.

Description: Extra-terrestrial colonization is of growing interest to space agencies and private entities, emphasizing the importance of research on reproduction and development in the absence of Earth's 1G. Maternal stressors can modify offspring development, exerting significant lifespan and crossgenerational changes through prenatal programming. The space environment is stressful, therefore exposure to altered gravity during pregnancy may impact later life outcomes in offspring. In ground-based studies, we exposed pregnant rats to continuous +G (above Earth gravity), and observed overweight and elevated anxiety in adult male (but not female) offspring, common phenotypes associated with prenatal maternal stress. Here we hypothesize that exposure to increased gravity during pregnancy elicits changes in the expression of stress-related genes in placenta that may mediate emergence of later life outcomes. While the placenta transports maternal factors to the fetus and produces endogenous fetal hormones, stress-induced changes at the placental-uterine interface may also alter communication between mother and fetus, facilitating prenatal transmission of unfavorable later life outcomes and cross-generational epigenetic alterations. Maternal stress elevates maternal glucocorticoids however placental 11b-hydroxysteroid dehydrogenase type 2 (HSD11B2) buffers fetal exposure by converting cortisol/corticosterone into inactive metabolites. Maternal stress during pregnancy down-regulates this enzyme and can induce epigenetic changes in placental and fetal tissues accounting for heightened adult HPA reactivity. Past studies have shown a placenta-specific increase in DNA methyltransferase (DNMT3a) mRNA in stressed mothers, an effect with implications for genome-wide epigenetic changes that may account for diverse phenotypic outcomes following maternal stress. Here we exposed groups of pregnant rats to one of five gravity loads (1, 1.5, 1.75 and 2G) and analyzed placental samples during late gestation. We predicted a systematic dose-response relationship between gravity load and the expression of the HSD11B2 and DNMT3 genes, thereby linking maternal exposure to altered gravity during pregancy with maternal stress.

Description: Spaceflight has several detrimental effects on the physiology of astronauts, many of which are recapitulated in rodent models. We analyzed liver transcriptomic and proteomic data from three mouse spaceflight experiments flown aboard the International Space Station (Rodent Research-1 NASA (RR-1 NASA), Rodent Research-1 CASIS (RR-1 CASIS), Rodent Research-3 (RR-3)), and one mouse experiment flown on the Space Shuttle (Commercial Biomedical Testing Module-3 (CBTM-3) aboard STS-135). Despite the differences in genetic background and time of exposure to microgravity it was shown through Oil Red staining and histology that increased lipid accumulation was occurring in the liver of all mice flown in space compared to the ground controls. This led to further pursue the existing GeneLab datasets related to liver omics data from these mice. We were able to discover key conserved pathways across all the mice independent of the flight conditions that were related to increased lipid metabolism, fatty acid metabolism, both lipid and fatty acid processing, lipid catabolic processing, and lipid localization. In addition, key upstream regulators were predicted to be commonly regulated across all conditions which include ESR1, GCG, and NR1I2 being inhibited and INS being activated. Interestingly, estrogen receptor alpha (ESR1) expression has been known to be heavily involved with lipoprotein metabolism. In addition, insulin (INS) is the primary driver for fat metabolism and increased INS has been associated with increased fatty acids in the liver. Through additional proteomic analysis we were able to identify the majority of the key proteins related to lipids for both the RR-1 and RR-3 rodents were being up-regulated in the livers when comparing flight to ground controls. This additional confirmation of the lipid associated activity also showed that the lipid related proteins are heavily involved with lipid metabolism, cholesterol binding, and cholesterol metabolism. Lastly, the analysis also revealed that the circadian clock related pathways in the liver are commonly being increased across all space flight conditions which has also been reported in the literature to potentially cause increased liver damage. The combination of the very strong lipid uptake in the liver and the transcriptomic/proteomic signatures (including the circadian clock pathways) following spaceflight are consistent with early onset of liver disease. Taken together, these data indicate that, activation of lipotoxic pathways could persist during longer duration spaceflight which might result in the development of liver disease

Description: In-situ food production is a necessary step for human exploration of the solar system and requires a deep understanding of plant growth in reduced gravity environments. In particular, the lack of buoyancy-driven convection changes the gas exchange at the leaf surface, which decreases photosynthesis and transpiration rates, and ultimately biomass production. To understand the intricate relations between physical, chemical, and biochemical processes, the following methodology combines the development of a mechanistic model of plant growth in reduced gravity environments, computational fluid dynamics (CFD) simulations, and experiments in different time frames.The model presented here is a coupled mass and energy balance using the single round leaf assumption, including gravity as an entry parameter, and the leaf surface temperature as an output variable. Measures of the leaf surface temperature using infra-red cameras allow for a computation of the transpiration rate. This approach was followed to design a parabolic flight experiment, which performed 7 flights, and enabled data collection for model validation in different gravity and ventilation settings on a short time frame. Current measures of carbon assimilation and transpiration rate at the leaf and canopy level using an infra-red gas analyzer (Li-6800) in 1g lab conditions on several species will enable a validation on longer time frames and further calibration of the model. CFD studies both on the parabolic flight and on the lab experimental set-up allow the precise assessment of ventilation above the canopy and plants' leaves.Ultimately, this work will provide recommendations for the design of future plant growth hardware, especially on the lowest adequate ventilation for optimal plant growth in reduced gravity environments, as well as assessing biomass and oxygen production rates on planetary surfaces and space stations. This work was funded by CNES, CNRS, Clermont Auvergne Metropole, and NASA Space Biology through NASA postdoctoral program / USRA.

Description: Research on human acclimation to spaceflight, including the recent NASA's Twin Study, reports complex effects of the spaceflight environment on health, with both acute and prolonged changes in multiple tissues. Spaceflight includes multiple factors such as microgravity, ionizing radiation, physiological stress, and disrupted circadian rhythms, that have been shown to contribute to pathophysiological responses that target immunity, bone and muscle integrity, cardiovascular and nervous systems. In this study, we used a well-established spaceflight model organism, Drosophila melanogaster, to assess spaceflight-associated changes on the nervous system. With 75% disease gene orthology to humans, short generation time, large sample size and ease of genetic, neuronal and behavioral studies, Drosophila is an excellent model to study nervous system dysfunction. Here, we present results from MVP-Fly-01 spaceflight mission that was launched on SpaceX CRS-14. The MVP hardware (developed by Techshot) used in this mission enabled us to have an in-flight 1g centrifuge, to distinguish the changes resulting from gravity versus those induced by other environmental factors associated with spaceflight. We observe behavioral impairments (p〈0.001) and synaptic deficits, including decreased synaptic connections (p〈0.05), in 3rd instar larvae which were developed in space. Furthermore, space-grown microgravity adults show a decrease in neuronal (p〈0.05) and dendritic field (p〈0.01) in adult brains coupled with an increased number of apoptotic cells (p〈0.001) compared to in-flight 1g controls, suggesting increased neuronal loss under spaceflight conditions. In summary, we observe that altered gravity leads to gross neurological deficits. To better understand the long-term effects of spaceflight on the nervous system, longitudinal and multigenerational changes were also identified. This study will help elucidate the different approaches to prevent nervous system dysfunction in astronauts during spaceflight, while also contributing to a better understanding of the pathways that are related to some CNS disorders on Earth.

Description: Spaceflight poses many challenges for humans. Ground-based analogs typically focus on single parameters of spaceflight and their associated acute effects. This study assesses the long-term transcriptional effects following single and combination spaceflight analog conditions using the mouse model, simulated microgravity via hindlimb unloading (HLU) and/or low-dose irradiation (LDR) for 21-days, followed by 4 months of readaptation. Changes in gene expression and epigenetic modifications in whole brain samples during readaptation were analyzed by DESeq2 and reduced representation bisulfite sequencing (RRBS). The results showed minimal gene expression alterations at 4-months within single treatment conditions of HLU and LDR. Following combined HLU+LDR, gene ontology and methylation analyses showed multiple altered pathways involved in neurogenesis and neuroplasticity, regulation of neuropeptides and cellular signaling. In brief, neurological readaptation following combined chronic LDR and HLU is a dynamic process that impacts brain structure and function and may lead to late onset neurological sequelae

Description: The Advanced Plant Habitat (APH) was installed on the International Space Station (ISS) in October 2017. Following a successful EVT (Experiment Verification Test) study at Kennedy Space Center (KSC), using Arabidopsis lines with varying levels of lignin, two inaugural studies were carried out on ISS in 2018 under the same experimental design, with the corresponding ground controls at KSC. The APH for this study deploys a substrate-based root module designed for plant growth in microgravity. Upon experiment initiation (such as for the EVT), the root module is primed (liquid imbibition) by flooding the root zone to initiate seed germination and to remove air from the porous tubing and particulate media. In the APH ISS inaugural study, the speed of supplying water to initially dry media was found to adversely affect the overall moisture distribution within the root module in microgravity (but not at 1g). Non-destructive estimations of Arabidopsis plant growth were carried out by monitoring changes in rosette leaf area on a daily basis. These data indicated that the original priming procedure caused patchy moisture distribution that affected plant growth and survival. An improved methodology for priming the second root module of PH-01 was devised and implemented in the second experiment. Leaf area and color estimates suggested that the modified priming scheme improved moisture distribution and plant growth. These data, when compared with the EVT study, suggest that nondestructive measurements of plant growth can aid towards optimization of plant growth conditions in microgravity.

Description: No abstract available

Description: Presentation is in support of NASA Kennedy Space Center (KSC) Speaker's Bureau Event (request #19-125546), which is about robotics and 3D printing as the new tools (vs. picks and shovels) for the lunar surface.

Description: Results of studies performed for entries into Saturn, Uranus, and Neptune atmospheres with HEEET as the candidateTPS. Margined material thicknesses are compared to loom limits, and CFD-based environments are used in material sizing.

Description: This presentation is a summary of the continuing effort to determine options for studying artificial gravity with rodents. Results of an engineering trade study are presented and an overview of past and planned short radius centrifugation studies are presented. A leading proposal for a future flight centrifuge capable of housing rodents, the Techshot RCF, is presented in only enough detail as is approved by Techshot for public domain use.

Description: GeneLab must establish data processing pipelines for common data types including microarray, RNA-sequencing, and metagenomic profiling. Here we give an overview of current microarray and RNA-seq pipelines and discuss future pipelines including metagenomic profiling pipelines

Description: Recent interest in human-scale missions to Mars has motivated the need for high-fidelity simulations of reentry flows. During a dust storm, there can be high levels of suspended dust in the Martian atmosphere, which cannot only enhance erosion of thermal protection systems but also transfer energy and momentum to the shock layer, thereby significantly augmenting the surface heat flux. Second-order finite-volume schemes are typically employed for hypersonic flow simulations, but such schemes suffer from a number of disadvantages. An attractive alternative is discontinuous Galerkin methods, which benefit from arbitrarily high spatial order of accuracy, geometric flexibility, and other properties. To enable accurate computations of high-speed particle-laden flows, an Euler-Lagrange methodology was developed in which the Eulerian field of the carrier gas is calculated using a discontinuous Galerkin scheme while the disperse phase is treated with Lagrangian particle tracking. We discuss challenges associated with coupling these two formulations and how to handle them. Momentum and energy transfer between the carrier gas and the particle phase is considered, and the importance of accounting for interparticle collisions is assessed. In addition, we describe the physical model of the particle phase and examine effects of its uncertainties on the numerical solution. We demonstrate the performance of the Euler-Lagrange method in representative testcases, with focus on the accurate prediction of particle trajectories and heating augmentation. Quantitative comparisons with experiments are provided.

Description: No abstract available

Description: The Lunar Reconnaissance Orbiter/Lyman Alpha Mapping Project (LAMP) ultraviolet instrument detected a 0.52% icy regolith mix on the floor of some of the southern pole permanently shadowed craters of the Moon. We present calculations indicating that most or all of this icy regolith detected by LAMP (sensed to a depth of 〈1 m) has to be relatively youngless than 2,000 years olddue to the surface erosional loss by plasma sputtering (external ionized gassurface interactions), meteoric impact vaporization, and meteoric impact ejection. These processes, especially meteoric impact ejection, will disperse water along the crater floor, even onto warm regions where it will then undergo desorption. We have determined that there should be a water exosphere over polar craters (e.g., like Haworth crater) and calculated that a model 40kmdiameter crater should emit ~10(exp 19) H2O per second into the exosphere in the form of free molecules and iceembedded particulates.

Description: No abstract available

Description: The Alfvn wave mode transmits fieldaligned currents and largescale turbulence throughout Jupiter's magnetosphere. Magnetometer data from the Juno spacecraft have provided the first observations of Alfvnic fluctuations along the polar magnetic flux tubes connected to Jupiter's main auroral oval and the Jovian satellites. Transverse magnetic field perturbations associated with Io are observed up to ~90 away from main Io footprint, supporting the presence of extended Alfvnic wave activity throughout the Io footprint tail. Additional broadband fluctuations measured equatorward of the statistical auroral oval are composed of incompressible magnetic turbulence that maps to Jupiter's equatorial plasma sheet at radial distances within ~20 R(sub J). These fluctuations exhibit a k(sub ||) power spectrum consistent with strong magnetohydrodynamic turbulence. This turbulence can generate up to ~100 mW/m(exp 2) of Poynting flux to power the Jovian aurora in regions connected to the inner magnetosphere's central plasma sheet.

Description: For over 100 years, neurologists have used eye movements to identify neural impairment, disease, or injury. Prior to the age of modern imaging, qualitative assessment of eye movements was a critical, routine component of diagnosis and remains today a routine law-enforcement tool for detecting impaired driving due to drugs or alcohol. We will describe the application of a simple 5-minute oculomotor tracking task coupled with a broad range of quantitative analyses of high-resolution oculomotor measurements for the sensitive detection of sub-clinical neural impairment and for the potential differentiation of various causes. Specifically, we will show that there are distinct patterns of impairment across our set of oculometric parameters observed with brain trauma, sleep and circadian disruption, and alcohol consumption. Such differences could form the basis of a self-administered medical monitoring or diagnostic support tool.

Description: As NASA's effort to establish a permanent residence in space continues, research on the effects of microgravity onbiological microorganisms is vital to protect or promote the health of plants and their astronaut counterparts. The purpose of this study is to determine the effects of microgravity on Pantoea agglomerans (P. agglomerans), using an analog microgravity simulator; the Rotary Cell Culture System (RCCS) developed at Johnson Space Center (JSC) in Houston, TX. P. agglomerans has been shown to be a plant growth promoter (PGPR) in ground based studies, but has also been shown to be a pathogen in both plants and immunocompromised patients. In this study, we will determine changes in the growth rate and antibiotic susceptibility of P. agglomerans when exposed to simulated microgravity.

Description: The STMD Lunar Surface Innovation Initiative (LSII) aims to spur the creation of novel technologies needed for lunar surface exploration and accelerate the technology readiness of key systems and components. The LSII activities will be implemented through a combination of unique in-house activities, competitive programs, and public-private partnerships.

Description: No abstract available

Description: Despite their numerical abundance and economic value, the behavior of many small coastal sharks in the US South Atlantic has been only coarsely described. Here we present movement summaries for blacknose (Carcharhinus acronotus), finetooth (C. isodon), and Atlantic sharpnose shark (Rhizoprionodon terraenovae) as they travelled through a regional-scale acoustic telemetry network, offering direct comparisons of habitat utilization, site fidelity, and the extent and timing of coastal migrations. From 2013-2016, 165 total sharks were implanted with acoustic transmitters at Cape Canaveral, Florida, and tracked up to four years. While blacknose sharks were common off east Florida year-round, finetooth sharks were most abundant winter through early spring and sharpnose sharks summer through fall. Blacknose sharks also moved more slowly (mean 0.8 kilometers per hour) and had the broadest depth preferences, while finetooth sharks were strongly shore-associated and sharpnose preferred proportionally deeper waters. All species exhibited low site fidelity when at Cape Canaveral, remaining at the same site for more than 1 hour on average, even when associated with deeper hard-bottom sites. Most finetooth and many blacknose undertook spring migrations as far as Virginia and North Carolina, respectively, before returning to east Florida each winter. Sharpnose also made regular northward movements that were not as obviously seasonally-driven. Multiple individuals of all species, particularly females, returned briefly south to Cape Canaveral in mid-summer, illustrating that coastal migrations in these species are more akin to seasonal expansions of their geographic ranges as opposed to a synchronized shift of the entire population along the coast.

Description: The S contents of rocks and soils are indicative of various alteration processes on Mars, e.g.[1]. It has been quantified along traverses at 4 landing sites Pathfinder, both MERs and MSL by the APXS [2,3]. At the MSL and MER sites, sulfur abundances, correlations with likely bound cations and other elements, and complementary mineralogical and textural data have provided important insights into alteration processes and periods of more habitable environments in the distant past.

Description: The rodent hindlimb unloading (HU) model was initially developed to simulate the cephalad fluid shift and musculoskeletal disuse in astronauts. Since then, the HU model has been applied to explore how other systems (e.g. immune, cardiovascular and CNS) respond to weightlessness. Most HU studies are performed with singly-housed animals, although social isolation also can substantially impact behavior and physiology, and therefore may confound HU experimental results. We hypothesized that relative to social housing, single housing exacerbates HU-induced dysfunction in select organ systems. We refined the standard NASA-Ames HU model to accommodate social housing in HU pairs, retaining advantageous features of traditional housing but using commercial off-the-shelf components to facilitate adoption by others. We conducted a 30 day HU experiment with adult, female C57Bl6/NJ mice that were either singly or socially housed. HU animals in both single and social HU housing displayed expected musculoskeletal deficits compared to housing matched, normally loaded (NL) controls. However, select immune, HPA axis, and CNS responses were differentially impacted by the HU social environment relative to NL controls. HU reduced % CD4+ T cells in singly-housed, but not socially-housed mice. Surprisingly, HU increased adrenal gland mass in socially-housed but not singly-housed mice, while social isolation increased adrenal gland mass in NL controls. HU also increased plasma corticosterone levels (day 30) in both singly and socially-housed mice. Thus, the social environment altered select adrenal and immune, but not musculoskeletal, responses to simulated weightlessness. We refine our original hypothesis since our results show combined stressors can mask, not only exacerbate, tissue responses to HU. These findings further expand the utility of the HU model for studying possible combined effects of the various spaceflight stressors.

Description: The effects of microgravity, and social isolation on the CNS are poorly understood. We hypothesize that mitochondrial reactive oxygen species (ROS) play an important role in this process. Since mice are social animals, our lab developed a novel social model of hindlimb unloading (HU), enabling us to determine the effects of both social isolation and simulated microgravity. Responses to 30d of HU were compared in wildtype or transgenic MCAT mice who over-express human catalase in mitochondria. Abundance of 4-Hydroxynonenal, Park7 (a redox-sensitive chaperone and sensor of oxidative stress) and corticosterone were measured by ELISA. Cytokines related to inflammation in the hippocampus and in plasma were analyzed by a protein array. Behavioral data was collected over a 24-hour period.Socially housed HU mice were more active and conducted at least two times more exploratory activities, compared to normally loaded mice. Correlation analysis revealed that specific brain and plasma cytokines correspond with specific behaviors. Simulated microgravity and/or social isolation caused changes in cytokine patterns in the hippocampus and in plasma, with significant interaction effects of HU and genotype in expression levels of five cytokines (out of 35). Interestingly, elevation of these generally pro-inflammatory cytokines by HU in WT mice was mitigated in MCAT mice, suggesting a role for mitochondrial ROS signaling in inflammatory CNS responses to microgravity. Interestingly, socially housed mice had also lower level of 4HNE and higher level of Park7 in the hippocampus compared to singly housed animals. The cytokine responses to social isolation were more extensive in brain vs plasma. Further, there was no overlap in the cytokine repertoire regulated in response to microgravity versus, isolation suggesting divergent mechanisms or downstream signaling. These findings implicate a potentially important role for mitochondrial ROS in CNS responses to the challenges posed both by prolonged missions in space and bedrest on Earth

Description: Spaceflight and the ensuing fluid shifts, together with an overall reduction in physical activity, lead to acute and latent effects on the cardiovascular system. This current study makes use of the rodent hindlimb unloading (HU) model to determine how factors such as sex, age, and duration of exposure impact cardiac responses to weightlessness. We hypothesize that extended exposure to simulated weightlessness and the ensuing recovery alters cardiac structure and expression of select genes, including those involved in redox signaling which together, negatively impact long-term cardiac tissue health. To begin to test this hypothesis, male and female rats underwent HU at various durations up to 90 days, with a subset reambulated after 90 days of HU. Physiological stress or contractility changes lead to alterations in ventricular cardiomyocyte size and ventricular wall thickness to adapt to greater functional demand and mitigate mechanical stress to ventricular tissue; under certain conditions, these changes also may mark progression to cardiac failure. Hence, left ventricular cardiomyocyte size (cardiomyocyte cross sectional area, CSA) was quantified to determine if HU leads to structural adaptation responses in cardiac tissue and if age and sex had any impact on this outcome. Cardiomyocyte CSA of older males (9 months) were altered by HU in a time-dependent manner, where HU led to decreases in CSA at 14 days and increases at 90 days. In contrast, younger males (3 months) did not show any changes at day 14 of HU. CSA of females (3 months) was increased in response to short-term HU (14 days) suggesting sex-dependence of structural changes. In older HU males, cardiomyocyte CSA was comparable to controls after 90 days of re-ambulation. Levels of the DNA oxidative damage marker, 8-hydroxydeoxyguanosine (8-OHdG) were greater in left ventricular tissue of females that underwent HU compared to sex-matched controls, while there were no such differences in older or younger males. To gain insight into the signals that drive cardiac adaptations to HU, global transcriptomic analysis (RNAseq) was performed on left ventricular tissue of older males that underwent 14 days of HU. Short-term simulated weightlessness led to differential expression of genes involved in immune and pro-inflammatory signaling. A subset of these genes play a role in autoimmune and cardiovascular disease and are targets of current drugs used to treat bradycardia, hypertension, atherosclerosis and rheumatoid arthritis, amongst others. Oxidative damage/redox signaling pathways were not enriched at the timepoint tested in older males. Since young females displayed greater oxidative damage to DNA, activation of oxidative stress responses at earlier or later time points cannot be ruled out. In summary, simulated weightlessness in adult rats caused changes in cardiomyocyte structure in a sex and age-dependent manner, and the transcriptional regulation of key mediators of immunity and cardiovascular disease, meriting further study to define cardiac risks for interplanetary travel of human crew. Our findings also confirm the value of the rat HU model for cardiac health and countermeasure research.

Description: Future long-duration missions face significant challenges maintaining crew health. A critical area is supplying adequate nutrition, as certain vitamins and nutrients in supplied foods and supplements demonstrate substantial degradation during extended storage. To address this issue, we are developing and flight-testing a platform technology that demonstrates in situ microbial production of targeted nutrients over extended mission durations. This 5-year experiment, known as BioNutrients-1, was started on the International Space Station in May 2019. It involves two components: an on-orbit hydration and production experiment; and the development of space-compatible, key bio-manufacturing microorganisms. On-orbit testing utilizes a small production pack system that encloses sterile edible growth substrate and desiccated Saccharomyces cerevisiae strains genetically engineered to produce the nutrients beta-carotene or zeaxanthin. On hydration and mixing of the production pack, the organisms revive and grow until limited by the depletion of growth media, hypothetically leading to consistent amounts of biomass and nutrients. In eventual mission applications, the packet contents would be heat treated to inactivate the microorganisms prior to consumption. For these flight experiments, the packet will not be heat treated, but will instead be frozen for return to Earth for analyses. In addition to the production pack trials, 14 different microorganisms/treatments were also delivered to ISS for long-duration storage. These samples will be intermittently returned to Earth and analyzed to determine survival rates and genomics. For this presentation, initial data from returned samples and ground controls will be discussed.

Description: Plant associated microbiomes, the rhizosphere and phyllosphere, are composed of communities of bacteria and fungi that may be mutualistic or pathogenic. These communities have the potential to influence plant health and development and can affect plant growth. Crop plants are being investigated as a fresh and safe supplement to astronauts diet and it is critical to understand and characterize these microbial communities. Multi-species crops, Mizuna mustard (Brassica rapa var japonica), Outredgeous red romaine lettuce (Lactuca sativa), and Waldmans Green lettuce (Lactuca sativa) were grown in two Veggie units on the International Space Station (ISS) for three grow outs in various combinations of plant types. Upon harvest, plant and pillow samples were frozen and returned to Earth for analysis. Bacterial and fungal community analyses for plant leaf and root, as well as pillow components, wick and media, were completed using next generation sequencing with the goal of surveying the composition of the entire community and identifying any potential pathogens. Bacteria were identified using the 16S rRNA gene whereas, fungi were identified with the internal transcribed spacer (ITS). The community composition for these three crops was compared between crop types and between plant tissue types. It is vital to mission success for the short term and long term to add nutritious, safe to eat vegetables providing a supplement to the crew members dietary requirements as well as to develop planning for deep space missions as we reach for the moon and on to Mars. Veggie technology validation tests were supported by NASAs Space Biology Program.

Description: It is important to determine the health risks and potential survival for astronauts associated with long-term space missions. This entails not only understanding the impact the space environment will have on humans, but also how it will affect other organisms needed for humans to survive in space such as plants. In addition, it has been reported in the literature that hundreds of genes seem to be conserved and/or transferred between different organisms from bacteria, archaea, fungi, microorganisms, and plants to animals. Since space travel involves humans in a closed environment over a long period of time, we hypothesize that potential conserved biological factors will occur between the different organisms in that environment possibly due to transfer of genes. Determining the conserved factors that are commonly being regulated in space can shed insight into possible universal master regulators and also determine the symbiotic relationship between the organisms in space. Utilizing NASA's GeneLab Data Repository (a rapidly expanding, curated clustering of spaceflight-related omics-level datasets for all organisms), we were able to uncover a novel pathway and factors that were commonly shared between humans, mice, plants, C. Elegans, and drosophilas. Through ChIP-Seq enrichment analysis techniques utilizing various GeneLab datasets from each species that were flown in space, we found the following factors to be conserved across all species: oxidative stress, DNA damage (through GABPA/NRFs and NFY), SIX5, GTF2B and glutamine synthetase. Such commonalities would likely reflect the effects of factors such as microgravity and the increased radiation exposure inherent in spaceflight on basic physical processes shared by all biological systems at the cellular level. Differences between organismal responses revealed by GeneLab's data should also help understand the unique reactions to life in space that arise from the very different lifestyles of microbes, animals and plants.

Description: A comprehensive understanding of the effects of spaceflight and altered gravity on human physiology is necessary for continued human space exploration and long-term space habitation. The oxidative stress response has been identified in astronauts exposed to short- and long-term space missions that are exposed to the multitude of stress factors of spaceflight, including altered gravity and radiation exposure. Reactive oxygen species (ROS) are byproducts of homeostatic cellular metabolism, yet when overproduced the oxidative stress response ensues, rendering molecules destructive causing cell death and inflammation. Controlling aberrant ROS production is necessary to prevent pathological consequences, in particular within the nervous system, since neurons are extremely sensitive overexpressed ROS insults. We hypothesize that exposure to altered gravity triggers the oxidative stress response, leading to impairments in the nervous system. In this study, we used a well-established spaceflight model organism, Drosophila melanogaster, to assess altered gravity associated changes in the nervous system using a ground-based hypergravity model. Acute hypergravity resulted in an induction of oxidative stress-related genes with an increase in reactive oxygen species (ROS) in fly brains (p〈0.001). Also, qPCR analysis shows that parkin gene expression is significantly reduced in these fly brains(p〈0.05). Additionally, chronic hypergravity resulted in depressed locomotor phenotype in these flies (p〈0.05) in conjunction to decreased dopaminergic neuron counts (p〈0.0001) and increased apoptosis in these fly brains (p〈0.0001). Further, assessment of neurological changes, including the neuronal architecture, synaptic integrity and genetic regulation caused by hypergravity conditions were noted. Overall, our results validate chronic hypergravity simulation as a behavioral model to study spaceflight effects, and oxidative stress pathway as a potential avenue for countermeasure development for astronauts undergoing short- and long-term missions and for neurodegenerative research on Earth.

Description: With humans pushing to live further off Earth for longer periods of time, it is increasingly important to understand the changes that occur in biological systems during spaceflight whether these be astronauts, their microbial commensals, or their plant-based life support systems. In a three-part presentation, we discuss GeneLab and recent discoveries regarding the microbiota of spacecrafts and space-flown animals. Part 1: GeneLab: Open Science for Life in Space, Jonathan Galazka, NASA Ames Research Center To accelerate the pace of discovery from precious spaceflight biological experiments, NASA as develop the GeneLab data system (genelab.nasa.gov), which allows unfettered access to omics data from spaceflight and spaceflight relevant experiments. GeneLab houses metagenomic datasets from spacecraft and relevant spacecraft models. Users can download this data and associated metadata to make new discoveries about how microbial communities may change and adapt to spaceflight.

Description: High-LET ionizing radiation is a major occupational health hazard for astronauts, but risk assessment remains elusive due to limited epidemiological data. Identifying genetic factors modulating the individual radiation response may be the most effective strategy to provide individualized risk management for long-duration high-radiation missions. We have started tackling the challenge of predicting individual risks by identifying human genetic loci associated with various radiation sensitivity phenotypes in primary blood mononuclear cells from a relatively large healthy human cohort. To date, we have performed the isolation of PBMCs from 768 subjects of the same ethnicity, and irradiated PBMCs from 576 subjects with 1 and 3 particles/100m2 of 600 MeV/n 56Fe, 350 MeV/n 40Ar and 350 MeV/n 28Si ions. The phenotypes of interest were: number of radiation-induced foci (or RIFs), CellROX oxidative stress responses and cell death, at 4h and 24h following irradiation. We have observed a significant inter-individual variability at 0 Gy between the 576 studied subjects, with a mean fold difference between the 10% lowest and highest responders of 5.6 of RIFs/cell, 7.9 in mean CellRox intensity, and 9.3 in percentage of dead cells. In order to better assess genetic factors influencing DNA repair, we used a metric previously introduced by our group to sort out radiation sensitivity phenotypes in mice: i.e. the ratio of the first to the second slope of RIFs/cell (between 0 and 1, and between 1 and 3 particle/100m2). Preliminary data on 192 individuals showed a distribution of low-dose responders (ratio 〉 1) to high-dose responders (ratio 〈 1) at 4h of 12%, 55% and 52% respectively for Fe, Ar and Si. The average value for the first and the second slopes was very similar for the two lowest LET (0.10 [-0.26;0.58] and 0.09 [-0.45;0.41] for Ar, 0.07 [-0.27;0.38] and 0.08 [-0.19;0.42] for Si), indicating a linear dose response across both fluence. Fe showed clear saturation for the highest dose with a slope of -0.09 [-0.86;1.51] against 0.68 [-2.21;2.20] for the low dose range, which probably reflects that many PBMCs are beyond repair at the high dose. Note that other significances were found for additional factors such as BMI and age whereas none were found for sex. GWAS will be performed on all phenotypes upon completion of measurements.

Description: The NASA GeneLab project capitalizes on multi-omic technologies to maximize the return on spaceflight experiments. To do this, GeneLab maintains a publicly accessible database (GLDS) that houses spaceflight and spaceflight relevant multi-omics data, and collaborates with NASA principal investigators and projects to generate additional omics data. GeneLab houses more than 200 transcriptomic, proteomic, metabolomic and epigenomic datasets from plant, animal and microbial experiments, with a growing number of these having been produced by the GeneLab sample processing lab. The GLDS contains rich metadata about each experiment and has recently integrated radiation dosimetery data from experiments flown on the Space Shuttle. GeneLab has also recently implemented an effort to present processed data in the GLDS in addition to the raw omics data. The processed data will enable interpretation of the data by a larger group of students, scientists and the general public. Standard pipelines for the transformation of raw data into visualizations were developed by four GeneLab Analysis Working Groups (animals, plants, microbes, multi-omics) comprised of over 100 scientists from NASA and academia. These pipelines are now being used by a group of bioinformatics interns to provide standard basic analysis of the data for incorporation into GLDS.

Description: This study maps and measures assorted properties of new dated crater clusters that formed recently when impactors fragmented in the atmosphere of Mars. We report these statistics for 77 clusters: number of craters, size of cluster, dispersion of cluster, direction (azimuth) from which the impactor approached, and an estimate of the angle from vertical of the impact. Clusters range from a few to hundreds of craters, with most containing tens of craters. They are most commonly dispersed over hundreds of meters, with extents ranging from a few meters to a few kilometers. We find that dispersion generally does not correlate with topographic elevation. However, when the highest elevations are disregarded, clusters are more dispersed at lower elevations, as expected. Impact azimuths are randomly distributed and do not express a clear directionality of incoming meteoroids. Results suggest impacts occur closer to horizontal than expected, which could be due to observational effects. The characteristics we report here provide important constraints for future work in understanding atmospheric fragmentation processes; properties of the impactors themselves, such as density and orbital parameters; and the seismic detectability of impacts. These are critical aspects to understand, as approximately half of current impacts are observed to be clusters.

Description: Extraterrestrial delivery of cyanide may have been crucial for the origin of life on Earth since cyanide is involved in the abiotic synthesis of numerous organic compounds found in extant life; however, little is known about the abundance and species of cyanide present in meteorites. Here, we report cyanide abundance in a set of CM chondrites ranging from 50 1 to 2472 38 nmolg1, which relates to the degree of aqueous alteration of the meteorite and indicates that parent body processing inuenced cyanide abundance. Analysis of the Lewis Cliff 85311 meteorite shows that its releasable cyanide is primarily in the form of [FeII (CN)5(CO)]3 and [FeII(CN)4(CO)2]2-. Meteoritic delivery of iron cyanocarbonyl complexes to early Earth likely provided an important point source of free cyanide. Iron cyanocarbonyl complexes may have served as precursors to the unusual FeII(CN)(CO) moieties that form the catalytic centers of hydrogenases, which are thought to be among the earliest enzymes.

Description: Spaceflight can cause immune system dysfunction, such as elevated white blood cells (WBC) and polymorphonuclear neutrophils (PMN), along with unchanged or reduced lymphocyte counts. A high PMN to lymphocyte ratio (NLR) can acts as a poor prognosis in cancer and a biomarker for subclinical inflammation however, the NLR has not been identified as a predictor of astronaut health during spaceflight. CBC data collected on board the International Space Station (ISS) was repurposed to determine the granulocyte to lymphocyte ratio (GLR) in humans and the NLR in rodents. The results displayed a progressive increase in GLR and NLR during spaceflight and at landing. The mechanism for increased NLR was assessed in vitro using the microgravity-analog, rotating wall vessel (RWV), with human WBCs. The results indicated that simulated microgravity led to increased GLR and NLR profiles, and production of reactive oxygen species (ROS) and myeloperoxidase (MPO). Interestingly, simulated microgravity increased the number of matured PMNs that showed impaired phagocytic function, while treatment with tert-Butyl hydroperoxide (TBHP), also reduced PMN phagocytosis. In addition, 30-days of simulated microgravity (hindlimb unloading) in mice, indicated an increased NLR and MPO gene expression, which were mitigated in mitochondrial catalase overexpressing transgenic mice, suggesting ROS scavenging is essential for maintaining homeostatic immunity. Collectively, we propose that the health status of astronauts during future short- and long-term space missions can be monitored by their NLR profile, in addition to utilizing this measurement as a tool for oxidative stress response countermeasure development to restore homeostatic immunity.

Description: Our hypothesis is that the higher-albedo rocks on the surface of Bennu are products of thermal/mechanical weathering. If this suggestion is correct, then the higher albedo (~715%) of some rocks relative to the average background on Bennu (~4.5%) could be explained by their relatively fresh surfaces. Older rocks on Bennu would then be the darkest rocks those that have experienced the most space weathering over time due to exposure to the space environment. Thermal/mechanical weathering (cracking of rocks) is a candidate mechanism for the particle ejection events observed to occur with regular frequency from the asteroid surface.

Description: High obliquity planets represent potentially extreme limits of terrestrial climate, as they exhibit large seasonality, a reversed annual-mean pole-to-equator gradient of stellar heating, and novel cryospheres. A suite of 3D global climate model simulations is performed for low and high obliquity planets with various stellar fluxes, CO2 concentrations, and initial conditions to explore the propensity for high obliquity climates to undergo global glaciation. We also simulate planets with thick CO2 or H2 atmospheres, such as those expected to develop near or beyond the outer edge of the habitable zone. We show that high obliquity planets are hotter than their low obliquity counterparts due to ice-albedo feedbacks for cold climates, and water vapor in warm climates. We suggest that the water vapor greenhouse trapping is greater on high obliquity bodies for a given global-mean temperature due to the different dynamical regimes that occur between the two states. While equatorial ice belts are stable at high obliquity in some climate regimes, it is substantially harder to achieve global glaciation than for a low obliquity planet. Temperate polar conditions can be present at high obliquity at forcings for which low obliquity planets would be in a hard snowball state. Furthermore, open ocean can persist even in the winter hemisphere and when global-mean temperatures are well below freezing. However, the influence of obliquity diminishes for dense atmospheres, in agreement with calculations from 1D energy balance models.

Description: The Origins, Spectral Interpretation, Resource Identification, and Security-Regolith Explorer (OSIRIS-REx; or OREx) spacecraft arrived at its target, near-Earth asteroid (101955) Bennu, on December 3, 2018. The OSIRIS-REx spacecraft has since collected a wealth of scientific information in order to select a suitable site for sampling. Shortly after insertion into orbit on December 31, 2018, particles were identified in starfield images taken by the navigation camera (NavCam 1). Several groups within the OSlRlS-REx team analyzed the particle data in an effort to better understand this newfound activity of Bennu and to investigate the potential sensitivity of the particles to Bennu's geophysical parameters. A number of particles were identified through automatic and manual methods in multiple images, which could be turned into short sequences of optical tracking observations. Here, we discuss the precision orbit determination (OD) effort focused on these particles at NASA GSFC, which involved members of the Independent Navigation Team (INT) in particular. The particle data are combined with other OSIRIS-REx tracking data (radiometric from OSN and optical landmark data) using the NASA GSFC GEODYN orbit determination and geodetic parameter estimation software. We present the results of our study, particularly those pertaining to the gravity field of Bennu. We describe the force modeling improvements made to GEODYN specifically for this work, e.g., with a raytracing-based modeling of solar radiation pressure. The short-lived, low-flying moonlets enable us to determine a gravity field model up to a relatively high degree and order: at least degree 6 without constraints, and up to degree 10 when applying Kaula-like regularization. We can backward- and forward-integrate the trajectory of these particles to the ejection and landing sites on Bennu. We assess the recovered field by its impact on the OSIRIS-REx trajectory reconstruction and prediction quality in the various mission phases (e.g., Orbital A, Detailed Survey, and Orbital B).

Description: No abstract available