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  • Triticum aestivum  (119)
  • Springer  (119)
  • American Association for the Advancement of Science (AAAS)
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  • 2005-2009
  • 1990-1994  (119)
  • 1950-1954
  • 1993  (59)
  • 1990  (60)
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  • Springer  (119)
  • American Association for the Advancement of Science (AAAS)
  • American Chemical Society
  • American Chemical Society (ACS)
  • Copernicus
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  • 2005-2009
  • 1990-1994  (119)
  • 1950-1954
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  • 1
    ISSN: 1570-7458
    Keywords: LepidopteraEphestia kuehniella ; α-amylase ; Triticum aestivum ; endosperm ; α-amylase inhibitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Crude α-amylase preparations from seven Lepidoptera pests were susceptible to inhibition by salt-soluble proteins of bread wheat (Triticum aestivum L.) endosperm. Protein fractions that corresponded to tetrameric, dimeric, and monomeric wheat α-amylase inhibitors, were decreasingly effective against the insect α-amylase activity. To further confirm these results, purified inhibitors were tested against an α-amylase preparation fromEphestia kuehniella (Zeller). This preparation showed decreased activity when increasing amounts of an heterotetrameric inhibitor (reconstituted from its isolated subunits WTAI-CM2, -CM3 and -CM16) were assayed. Activity was only partially inhibited by homodimeric (WDAI-1, synonym 0.53; WDAI-2, synonym 0.19) and monomeric (WMAI-1, synonym 0.28) inhibitors.
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  • 2
    ISSN: 1432-203X
    Keywords: cryopreservation ; Triticum aestivum ; abscisic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Spring wheat (Triticum aestivum L.) zygotic embryos were successfully cryopreserved, without the addition of exogenous cryoprotectants, using only an abscisic acid (ABA) pretreatment. Optimum survival was obtained when embryos were cultured in vitro for 10 days on semisolid Murashige and Skoog (MS) nutrient medium supplemented with 0.5 mg/L (±) ABA prior to cryopreservation. The embryos resumed growth within three days when returned to MS medium devoid of ABA but containing 2mg/L 2,4-dichlorophenoxyacetic acid. The embryogenic calli produced from these embryos exhibited normal plant regeneration on auxin-free media. Changes in dw/fw ratio, as well as the esterified fatty acid and sucrose concentrations correlated positively with the development of tolerance to cryopreservation.
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  • 3
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    Plant cell reports 12 (1993), S. 149-153 
    ISSN: 1432-203X
    Keywords: Triticum aestivum ; microspore culture ; ovary co-culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Wheat microspores were isolated, without prior anther culture, from a range of genotypes and cultured to regenerate self-fertile plants. Microspores were isolated using a microblender and competent microspores were enriched by gradient centrifugation. The use of maltose as the sole carbohydrate in the culture medium and co-culture of microspores with wheat or barley ovaries were critical for development of microspore-derived embryos. Results were also improved when spikes were pretreated by emersion of the basal ends of detached heads in water at 25°C for 2d. This procedure leads to highly reproducible production of plants.
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  • 4
    ISSN: 1432-0983
    Keywords: Triticum aestivum ; Secale cerele ; Ribosomal RNA genes ; Mitochondrial DNA ; Recombining-repeats ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial genomes of wheat and rye each contain a three-member family of recombining repeat peat sequences (the “18S/5S repeat”) that encode genes for 18S and 5S rRNAs (rrn18 and rrn5) and tRNAfMet (trnfM). Here we present, for wheat and rye, the sequence and boundaries of the “common sequence unit” (CSU) that is shared between all three repeat copies in each species. The wheat CSU is 4,429 base-pairs long and contains (in addition to trnfM, rrn18 and rrn5) a putative promoter, three tRNA-like elements (“t-elements”), and part of a pseudogene (“ψatpA c”) that is homologous to chloroplast atpA, which encodes the α subunit of chloroplast F1 ATPase. The rye CSU is somewhat smaller (2,855 base pairs) but contains much the same genic and other sequence elements as its wheat counterpart, except that two of the three t-elements as well as ψatpA c are found in only one of the three downstream flanks of the 18S/5S repeat, outside the CSU boundaries. In interpreting the seuuence data in terms of the evolutionary history of the 18S/5S-repeat family of wheat and rye, we conclude that (1) the wheat-rye form of the 18S/5S repeat most likely originated between 3 and 14 million years ago, in a lineage that gave rise to wheat and rye but not to barley, oasts, rice or maize; (2) the close linkage (1-bp apart) between trnfM and rrn18 is similarly limited in its taxonomic distribution to the wheat/rye lineage; (3) the trnfM-rrn18 pair arose via a single mutation that inserted a sequence block containing trnfM immediately upstream of rrn18; and (4) the presence of a putative promoter upstream of rrn18 in all wheat and rye repeats is consistent with all three repeat copies being transcriptionally active. We discuss these conclusions in the light of the possible functional significance of recombining-repeats in plant mitochondrial genomes.
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  • 5
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    Plant molecular biology 21 (1993), S. 919-921 
    ISSN: 1573-5028
    Keywords: ARS-related element ; dispersed repeat ; genomic DNA sequence ; molecular evolution ; Secale cereale ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Hind III-generated fragment of wheat embryo nuclear DNA has been cloned and sequenced. The cloned fragment corresponds to a 1241 bp long, moderately repeated (60 000 copies/genome) segment of the genomic DNA. The repeat is AT-rich (67%), contains an open reading frame for 151 amino acids and several nucleotide blocks resembling the consensus domain of autonomously replicating sequences. Southern blot hybridization analyses indicate that the repeat is scattered through the wheat genome. A sequence homologous to this repeat occurs also in rye embryo nuclear DNA where it shows the same dispersion pattern as that observed for the wheat repeat.
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  • 6
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    Biochemical genetics 31 (1993), S. 75-86 
    ISSN: 1573-4927
    Keywords: Triticum aestivum ; Wx protein ; two-dimensional polyacrylamide gel electrophoresis ; Wx locus ; waxy mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Nullisomic analysis of waxy (Wx) protein of hexaploid wheat (Triticum aestivum L.) cv. “Chinese Spring” using two-dimensional polyacrylamide gel electrophoresis revealed that threeWx loci,Wx-A1, Wx-B1, andWx-D1, located on chromosome arms 7AS, 4AL, and 7DS, produce three distinct Wx subunit groups, subunit group-A (SGA), SGB, and SGD, respectively. SGA has a higher molecular weight and a more basic isoelectric point (pI) than the other two. SGB and SGD have the same molecular weight but a slightly different pI range. Owing to the detection of these three subunit groups, we were able to identify the expression of three waxy genes in wheat endosperm and to find two types of mutants among Japanese wheat cultivars, one lacking SGA and the others SGB. These results suggest the possibility of breeding a waxy wheat.
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  • 7
    ISSN: 1573-5028
    Keywords: wheat ; Triticum aestivum ; cDNA clone ; ADP-glucose pyrophosphorylase ; small-subunit ; starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A full-length cDNA clone from hexaploid bread wheat, encoding the small subunit of ADP-glucose pyrophosphorylase, has been isolated from an endosperm cDNA library. The cDNA insert has an open reading frame which encodes a protein of 473 amino acids (52.1 kDa). The presence of a chloroplast/amyloplast transit peptide of 22 amino acids is proposed. The deduced amino acid sequence exhibits a high degree of homology with the small subunit ADP-glucose pyrophosphorylase proteins from rice (with 90% of identical amino acids) and potato (with 86% of identical amino acids) and contains conserved sequence elements which are thought to represent the substrate binding and allosteric activator sites. The genes are organised as single-copy loci on chromosomes 7A, 7B and 7D in the wheat genome and are highly expressed during grain development. Homologous transcripts are expressed in leaves and roots.
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  • 8
    ISSN: 1432-2242
    Keywords: Aegilops ventricosa ; DNA probes ; Introgression lines ; Addition lines ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Stable wheat-Aegilops introgression lines with 42 chromosomes (H-93), derived by repeated selfing from a cross (Triticum turgidum x Aegilops ventricosa) x T. aestivum, have been characterized using the following DNA probes and isozyme markers: (1) single or low-copy DNA fragments from Ae. ventricosa; (2) known cDNA probes corresponding to α1-thionin, monomeric α-amylase inhibitor, the CM3 subunit of tetrameric α-amylase inhibitor, and sucrose synthase from wheat; (3) anonymous cDNA probes from wheat that have been mapped by Sharp et al. (1989); (4) isozyme markers corresponding to aconitase, shikimate dehydrogenase, adenylate kinase, and endopeptidase. Meiotic metaphases of appropriate hybrids involving selected H-93 lines have been investigated by the Giemsa C-banding technique. The substitution of whole chromosomes [(5A) 5Mv; (4D) 4Mv; (5D) 5Mv; (7D) 7Mv] and chromosomal segments (1Mv; 3Mv; 5Mv; 7Mv) from the Mv genome of Aegilops ventricosa has been demonstrated. The distribution of selected markers among putative wheat-Ae. ventricosa addition lines has also been investigated. The 7Mv addition has been characterized for the first time, while the identity of the previously reported 5Mv and 6Mv additions has been confirmed.
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  • 9
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; T. durum ; Aegilops longissimum ; Dasypyrum villosum ; Endonuclease ; Cytoplasm donor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To elucidate the phylogenetic relationships and cytoplasmic types, restriction endonuclease fragment patterns of chloroplast (cp) and mitochondrial (mt) DNAs isolated from two different accessions of Dasypyrum villosum (L.) candargy were compared with those of tetraploid wheat (Triticum durum Desf., PI265007), hexaploid wheat (Triticum aestivum L., cv Chinese Spring), Aegilops longissimum (S. and M., in Muschli) Bowden and Hordeum vulgare L. T. aestivum and T. durum had identical restriction patterns for their cp and mtDNAs in digestions with four different enzymes. Likewise, no differences were found between the restriction fragment patterns of two accessions of D. villosum. But, there were distinct differences in chloroplast and mitochondrial DNA restriction fragment patterns between D. villosum and tetraploid and hexaploid wheats. A. longissimum (G609) showed a similar pattern to those wheats for PstI digestion of cpDNA. Organellar DNA from Hordeum vulgare (cv Himalaya) showed a distinctly different restriction pattern from those of wheat and D. villosum. These results suggest that D. villosum is unlikely to be the donor of cytoplasm to wheats, and its cytoplasmic organelles were also different from those of A. longissimum.
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  • 10
    ISSN: 1432-2242
    Keywords: Grain softness ; Friabilin ; Milling quality ; Triticum aestivum ; Seed storage-proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Mr 15000 protein associated with water-washed wheat starch granules from soft wheats was shown to be heterogeneous: it could be divided into a fraction containing one or moreα-amylase inhibitor subunits and a fraction largely composed of a previously uncharacterised polypeptide(s) referred to as the “grainsoftness protein” (GSP). The major N-terminal sequence and sequences of peptides derived from protease digests of GSP are reported. An antiserum specific for GSP was used to show that GSP accumulated in both hard and soft wheat grains, but the GSP in soft grains associated more strongly with starch granules than the GSP in hard grains. A positive correlation between grain softness and accumulation of GSP in the seed was demonstrated for a range of cultivars. This differs from the qualitative relationship, based on the isolated starch fraction, between GSP and grain softness that has already been reported. Analysis of wholemeal extracts with the antiserum demonstrated that the accumulation of GSP in the seed was dependent on the short arm of chromosome 5D, which also encodes theHa locus. In addition, examination of near-isogenic lines differing in hardness indicated that the gene(s) controlling GSP was (were) linked with theHa locus. The findings indicate that GSP may be the product of theHa locus and thus be the major factor that determines the milling characteristics of bread wheats.
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  • 11
    ISSN: 1432-2242
    Keywords: Rye translocations ; Triticum aestivum ; Glutenin ; Gliadin ; Glu-3 loci ; Gli-1 loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A triple (1AL.1RS/1BL.1RS/1DL.1RS) and three double (1AL.1RS/1BL.1RS, 1AL.1RS/1DL.1RS, 1BL.1RS/1DL.1RS) wheat-rye 1RS translocation stocks were isolated from a segregating population using the Gli-1, Tri-1 and Sec-1 seed proteins as genetic markers. These stocks carried 42 chromosomes and formed the expected multivalents (frequency of 14–25%) at metaphase 1. They gave floret fertility ranging from 40–60%. These stocks were subsequently used to determine the genetic control of low-molecular-weight (LMW) glutenin subunits in ‘Chinese Spring’ and ‘Gabo’ by means of two-step one-dimensional SDS-PAGE. All of the B subunits and most of the C subunits of glutenin were shown to be controlled by genes on the short arms of group-1 chromosomes in these wheats. The other C subunits were not controlled by group-1 chromosomes. The triple translocation line served as a suitable third parent in producing test-cross seeds for studying the inheritance of the LMW glutenin subunits and gliadins in wheat cultivars, e.g. ‘Chinese Spring’ and ‘Orca’. The segregation patterns of the LMW glutenin subunits in these cultivars revealed that the subunits were inherited in clusters and that their controlling genes (Glu-3) were tightly linked with those controlling gliadins (Gli-1). The LMW glutenin patterns d, d and e in ‘Orca’ segregated as alternatives to the patterns a, a and a in ‘Chinese Spring’ controlled by Glu-A3, Glu-B3 and Glu-D3 loci on chromosome arms 1AS, 1BS and 1DS, respectively, thus indicating that these patterns were controlled by allelic genes at these loci.
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  • 12
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Thinopyrum bessarabicum ; Protein-isozyme markers ; Chromosome banding ; Intergeneric hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thinopyrum bessarabicum (2n = 2x = 14, JJ) with its unique property of salt tolerance provides a potential means for the transfer of this important and complex trait into cultivated wheat through intergeneric hybridization. To accomplish this, diagnostic markers for detecting the presence of Th. bessarabicum chromosomes in a wheat background have to be established. The C-banded karyotype of Th. bessarabicum distinctly identifies individual Th. bessarabicum chromosomes and separates them from those of Triticum aestivum. Also, seven protein/isozymes, i.e., malate dehydrogenase, high-molecular-weight glutenin, Superoxide dismutase, grain esterase, glutamate oxaloacetate transaminase, β-amylase and α-amylase, were identified as being positive markers specific to Th. bessarabicum; these were also expressed in the T. aestivum/Th. bessarabicum amphiploid. These diagnostic biochemical markers could be useful in detecting and establishing homoeology of Th. bessarabicum chromosomes in T. aestivum/Th. bessarabicum intergeneric hybrid derivatives.
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  • 13
    ISSN: 1432-2242
    Keywords: Gliadin loci ; Group 1 chromosomes ; Recombination ; Electrophoresis ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inheritance and biochemical properties of gliadins controlled by the group 1 chromosomes of the high-quality bread wheat cultivar Neepawa were studied in the progeny of the cross Neepawa x Costantino by six different electrophoretic procedures. Chromosome 1B of Neepawa contains two gliadin loci, one (Gli-B1) coding for at least six ω- or γ-gliadins, the other (Gli-B3) controlling the synthesis of gliadin N6 only. The map distance between these loci was calculated as 22.1 cM. Amongst the chromosome 1A gliadins, three proteins are encoded at the Gli-A1 locus whereas polypeptides N14-N15-N16 are controlled by a remote locus which recombines with Gli-A1. Six other gliadins are controlled by a gene cluster at Gli-D1 on chromosome 1D. Canadian wheat cultivars sharing the Gli-B1 allele of Neepawa were found to differ in the presence or absence of gliadin N6. The electrophoretic mobilities of proteins N6 and N14-N15-N16 were unaffected by the addition of a reducing agent during two-dimensional sodium dodecyl sulphate polyacrylamid-gel electrophoresis, suggesting the absence of intra-chain disulphide bonds in their structure.
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  • 14
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Mildew resistance ; Pm3 locus ; Near-isogenic lines ; RFLP marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this study was to identify molecular markers linked to genes for resistance to powdery mildew (Pm) in wheat using a series of ‘Chancellor’ near-isogenic-lines (NILs), each having one powdery mildew resistance gene. A total of 210 probes were screened for their ability to detect polymorphism between the NILs and the recurrent parent. One of these restriction fragment length polymorphism (RFLP) markers (Xwhs179) revealed polymorphism not only between the NILs for the Pm3 locus, but also among NILs possessing different alleles of the Pm3 locus. The location of the marker Xwhs179 was confirmed to be on homoeologous chromosome group 1 with the help of nullitetrasomic wheat lines. The linkage relationship between this probe and the Pm3 locus was estimated with double haploid lines derived from a cross between wheat cvs ‘Club’ and ‘Chul’ (Pm3b). The genetic distance was determined to be 3.3±1.9 cM.
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  • 15
    ISSN: 1615-6102
    Keywords: Triticum aestivum ; Roots ; Companion cells ; Colchicine ; Abnormal sieve elements ; Plastids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Companion cells (CCs) of seminal roots of wheat (Triticum aestivum L.) flank protophloem sieve elements (PSEs) on their inner side. In roots growing in the presence of 2 mM colchicine prospective CCs are induced to differentiate into abnormal sieve elements. The most convincing evidence that they deviate from their programmed developmental sequence is the deposition of dense crystalloid inclusions within their plastids, a feature characterizing sieve elements of gramineous species. These plastids, however, are ultrastructurally different from those of normal and abnormal PSEs in that they contain only one kind of crystalloid inclusion, exceptionally dense stroma, swollen vesicular structures and plastoglobuli. Mitochondria accumulate in large groups and appear unusually swollen and deformed. Walls develop abnormal thickenings which are deposited irrespective of the position of plasmodesmata. Nuclei become polyploid and appear highly lobed, but degenerate similarly to those in normal sieve elements. Aggregates of paracrystalline material occur in the cytoplasm between the nucleus and cell wall. The end walls display features characterizing developing sieve plates, callose is deposited around plasmodesmata, but sieve pores are not formed. Aberrant CCs may reach “maturity” and co-exist with colchicine-affected PSEs. Their arrangement may be similar to or differ from that of normal phloem poles. Not all cells in CC files are induced to deviate into abnormal sieve elements, nor does their development occur in sequence. Because developmental changes also occur in polyploid cells, which result from colchicine blocked cell divisions, it is concluded that cell division is not a prerequisite for cell differentiation.
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  • 16
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    Biochemical genetics 31 (1993), S. 75-86 
    ISSN: 1573-4927
    Keywords: Triticum aestivum ; Wx protein ; two-dimensional polyacrylamide gel electrophoresis ; Wx locus ; waxy mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Nullisomic analysis of waxy (Wx) protein of hexaploid wheat (Triticum aestivum L.) cv. “Chinese Spring” using two-dimensional polyacrylamide gel electrophoresis revealed that threeWx loci,Wx-A1, Wx-B1, andWx-D1, located on chromosome arms 7AS, 4AL, and 7DS, produce three distinct Wx subunit groups, subunit group-A (SGA), SGB, and SGD, respectively. SGA has a higher molecular weight and a more basic isoelectric point (pI) than the other two. SGB and SGD have the same molecular weight but a slightly different pI range. Owing to the detection of these three subunit groups, we were able to identify the expression of three waxy genes in wheat endosperm and to find two types of mutants among Japanese wheat cultivars, one lacking SGA and the others SGB. These results suggest the possibility of breeding a waxy wheat.
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  • 17
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    Journal of plant research 106 (1993), S. 239-244 
    ISSN: 1618-0860
    Keywords: Chromosome breakage ; Gametocidal gene ; Healing ; Telomere repetitive sequence ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Telomere formation of the normal and broken chromosomes of common wheat,Triticum aestivum, was investigated byin situ hybridization using the biotin-labeled probe of telomere repetitive sequences (pAtT4) ofArabidopsis thaliana with subsequent amplification by an antibody. After double and triple amplification, prominent signals appeared at all the telomeric regions of the normal chromosomes. Prominent signals also emerged at the broken ends of the telocentric and deletion chromosomes that had passed through more than one generation since the appearance. However, broken ends that had passed through only the stages of gametogenesis, fertilization, embryogenesis and root development did not show complete signals such as found in normal telomeres. These findings indicate that a certain time or stage is required for synthesis of the telomeric repetitive sequences with a complete length. Nevertheless, because the broken ends without complete telomere sequences were also healed, restoration of the normal complement of telomere sequences is not necessary for healing of broken ends.
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  • 18
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; lignin ; manganese ; Mn-efficiency ; phenolics ; roots ; resistance mechanisms ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Differential resistance of four Triticum aestivum L. genotypes to isolates of take-all fungus (Gaeuman-nomyces graminis var. ritici Walker) was tested in a complete factorial experiment set up in a growth chamber using Mn-deficient Wangary sand amended with four rates of Mn. Mn-efficient cultivars produced more dry matter at low supply of Mn. Fertilization with Mn significantly increased its accumulation in roots and shoots. The most sensitive measure of take-all infection was the total length of root stellar lesions; these lesions were reduced by Mn fertilization and were shorter in Mn-efficient genotypes. The resistance-enhancing effect of Mn was the most obvious in the Mn-inefficient genotype (Bayonet) and the least obvious in the Mn-efficient one (C8MM). Phenolics biosynthesis in roots was clicited by fungal infection, especially in the case of the highly virulent isolate. The weakly virulent isolate increased phenolics concentration in roots much more if no Mn was added, indicating that the resistance-enhancing effect of Mn may not be directly exerted through the effects on phenolics biosynthesis. Lignin concentration in roots decreased due to Mn fertilization, while no effect of take-all infection was noted. It appears that biosynthesis of phenolics and lignin in wheat roots has a low Mn requirement which can be satisfied at environmental Mn concentrations below those necessary for optimum plant growth. ei]Section editor: A C Borstlap ei]Section editor: H Lambers
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  • 19
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    Plant and soil 155-156 (1993), S. 489-492 
    ISSN: 1573-5036
    Keywords: aluminium ; analog ; boron ; copper ; gallium ; iron ; lanthanum ; manganese ; scandium ; tolerance ; Triticum aestivum ; toxicity ; wheat ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of aluminium (Al), manganese (Mn), zinc (Zn), copper (Cu), boron (B), iron (Fe), gallium (Ga), scandium (Sc) and lanthanum (La) on growth of an Al-tolerant and an Al-sensitive line of wheat (Triticum aestivum L.) were measured in solution culture. The concentrations of nutrients in the basal nutrient solution were (μM) 500 Ca, 100 Mg, 300 K, 600 N (150 NH4, 450 NO3), 600 SO4, 2.5 P, 3 B, 2.5 Fe, 0.5 Zn, 0.5 Mn, 0.1 Cu at a pH of 4.7. The major solution nutrient concentrations were maintained at the nominal concentration with monitoring, frequent additions and weekly renewal. Differentiation in yield between the Al-tolerant and Al-sensitive line only occurred in the presence of Al indicating that, in the long term, none of the other metals tested could be used as an analog for Al. The visual symptoms in the roots of Cu toxicity (in both lines) and Al toxicity (in the sensitive line) were similar. The solution concentration (μM) at which yield of the roots of the tolerant line was reduced by 50% was, in order of increasing tolerance, Cu 0.5, Sc 1.1, La 7.1, Ga 8.6, Al 15, Zn 19, Fe 84, B 490 and Mn 600.
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  • 20
    ISSN: 1573-5036
    Keywords: efficiency ratio ; harvest index ; potassium ; Triticum aestivum ; utilization index ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nutrient use-efficiency (NUE) is commonly measured in relation to vegetative growth without regard for economic productivity of crops whose valued product is reproductive. Although vegetative measures can be useful, particularly in forage crops, their validity in the quantification of NUE in grain crops is questionable. This study was undertaken primarily to examine the relationship between vegetative and economic potassium use-efficiency (KUE) in several wheat (T. aestivum) genotypes under conditions of potassium stress. Genotype environment interaction for vegetative KUE was also examined. Vegetative KUE was assessed as shoot fresh weight, efficiency ratio (VKER) and utilization index (VKUI) whereas economic KUE was evaluated as grain weight, total weight and economic efficiency ratio (EKER). Significant genotype-environment interactions for shoot weight, VKER and VKUI were observed. In some instances interaction was associated with crossovers between genotypes indicating that it can affect selection. Correlations between vegetative and economic measures of KUE were generally nonsignificant, but negative and significant for shoot weight of three-week-old plants and grain weight. It appears that if genotypes differ considerably for harvest index and it is not positively correlated with total biomass, vegetative measures of KUE are unreliable as indicators of economic KUE. Therefore, economic rather than vegetative measures should be used to evaluate KUE in crops whose valued product is reproductive. ei]{gnH}{fnMarschner} ei]{gnH.}{fnLambers}
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  • 21
    ISSN: 1573-5036
    Keywords: ammonium assimilation ; carbon partitioning ; nitrate assimilation ; nitrogen partitioning ; Triticum aestivum ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The carbon and nitrogen partitioning characteristics of wheat (Triticum aestivum L.) and maize (Zea mays L.) grown hydroponically at a constant pH on either 4 mM or 12 mM NO3 - or NH4 + nutrition were investigated using either 14C or 15N techniques. Greater allocation of 14C to amino-N fractions occurred at the expense of allocation of 14C to carbohydrate fractions in NH4 +-compared to NO3 --fed plants. The [14C]carbohydrate:[14C]amino-N ratios were 1.5-fold and 2.0-fold greater in shoots and roots respectively of 12 mM NO3 --compared to 12 mM NH4 +-fed wheat. In both 4 mM and 12 mM N-fed maize the [14C]carbohydrate:[14C]amino-N ratios were approximately 1.7-fold and 2.0-fold greater in shoots and roots respectively of NO3 --compared to NH4 +-fed plants. Similar results were observed in roots of wheat and maize grown in split-root culture with one root-half in NO3 --and the other in NH4 +-containing nutrient media. Thus the allocation of carbon to the amino-N fractions occurred at the expense of carbohydrate fractions, particularly within the root. Allocation of 14N and 15N within separate sets of plants confirmed that NH4 --fed plants accumulated more amino-N compounds than NO3 --fed plants. Wheat roots supplied with 15NH4 + for 8 h were found to accumulate 15NH4 + (8.5 μg 15N g-1 h-1) whereas in maize roots very little 15NH4 + accumulated (1.5 μg 15N g-1 h-1) It is proposed that the observed accumulation of 15NH4 + in wheat roots in these experiments is the result of limited availability of carbon within the roots of the wheat plants for the detoxification of NH4 +, in contrast to the situation in maize. Higher photosynthetic capacity and lower shoot: root ratios of the C4 maize plants ensure greater carbon availability to the root than in the C3 wheat plants. These differences in carbon and nitrogen partitioning between NO3 --and NH4 +-fed wheat and maize could be responsible for different responses of wheat and maize root growth to NO3 - and NH4 + nutrition.
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  • 22
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    Plant and soil 155-156 (1993), S. 453-456 
    ISSN: 1573-5036
    Keywords: copper deficiency ; cotton ; ELISA ; Gossypium hirsutum ; immunoassay ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Studies were carried out with hydroponically grown wheat and cotton to develop the Cu-requiring protein phenolase as a biomarker of Cu nutrient status. Isozymes of phenolase whose levels were reduced by Cu deficiency were identified by Western blots. A competitive enzyme-linked, immunosorbent assay (ELISA) was developed that could detect as little as 25 ng of phenolase. The ELISA revealed that Cu-sufficient cotton leaves had about 4-fold more phenolase antigen than did Cu-sufficient wheat leaves. In both species, the level of phenolase was reduced by 2- to 5-fold in leaves of Cu-deficient plants. Because the immunoassay for phenolase protein is rapid, inexpensive, and can be carried out with small amounts of leaf material, it has potential as a tool for assessment of the Cu status of crop plants.
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  • 23
    ISSN: 1573-5044
    Keywords: cell culture ; karyotype instability ; rDNA ; repetitive DNA ; retroelement ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rapidly growingTriticum aestivum L. (wheat) derived long term suspension culture (named TaKB1), that is probably not regenerable, was analysed for karyotype rearrangements, stability and changes in repetitive DNA. The cell line has an average chromosome number of 21 and the DNA amount of unreplicated cells of TaKB1 measured by flow cytometry is about 30% lower than an unreplicated (1C) bread wheat genome.In situ hybridization of a repetitive DNA sequence (pSc119.2), which occurs as tandemly repeated blocks (heterochromatin) in wheat, shows that chromosomes from the TakB1 line have fewer and weaker subtelomeric locations of the sequence than wheat, suggesting deletions of distal chromosome segments and a reduction in the sites and copy number of the sequence. Thein situ hybridization pattern and chromosome morphology allowed 27 chromosome types to be identified in the cell line. No two analysed cells contained the same chromosome complement, although some chromosome types were present in every cell. Using Southern hybridization the structure and copy number of a retroelement (Wis-2) and its flanking sequence was shown to be the same in the TaKB1 cell line and wheat. Anin situ analysis of rDNA in the TaKB1 cell line (using the probe pTa71) showed a reduction in number of sites and rRNA genes in each cell from that in wheat. Interphase cells of the cell line showed dispersed signal throughout the nucleolus with no evidence for clusters of condensed and inactive rRNA genes.
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  • 24
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    Euphytica 67 (1993), S. 41-48 
    ISSN: 1573-5060
    Keywords: Aegilops crassa ; hybrid wheat ; PCMS ; photoperiod-sensitive cytoplasmic male sterility ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Triticum aestivum cv. Norin 26 with Aegilops crassa, Ae. juvenalis or Ae. vavilovii cytoplasm (all D2 type) has been studied relative to its photoperiodic response of male sterility and fertility restoration patterns. Alloplasmic lines of ‘Norin 26’ with a D2 type cytoplasm showed almost complete male sterility under long-day conditions (≥15 h), but high male fertility under short-day conditions (≤14.5 h). No significant influence of temperature on reduction in male fertility was observed. Thus, this type of male sterility is called ‘photoperiod-sensitive cytoplasmic male sterility’ (PCMS). The PCMS is expressed in the form of pistillody of stamens. Histological studies revealed that there were incomplete ovule-like structures instead of tapetal cells and pollen grains in the pistillate stamens. The floret differentiation stage of the plant is the stage that is sensitive to photoperiod. The PCMS can be used as a new means for hybrid wheat production, named ‘two-line system’. The PCMS line is maintained and multiplied by self-fertilization under short-day conditions, and hybrid seed can be produced by crossing the PCMS line with a pollinator line under long-day conditions. In contrast to the system of hybrid wheat production using the T. timopheevi cytoplasm, the present system requires only PCMS and pollinator lines.
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  • 25
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    Euphytica 67 (1993), S. 49-57 
    ISSN: 1573-5060
    Keywords: aphid infestation ; bird cherry-oat aphid ; Rhopalosiphum padi ; cereal aphids ; insect resistance ; leaf pubescence ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In Hungary the bird cherry-oat aphid (Rhopalosiphum padi L.) is the most frequent aphid species in winter wheat (Triticum aestivum L.). Estimations of infestation by R. padi as well as measurements of grain yield and thousand-kernel mass were carried out in 26 winter wheat genotypes in conditions of naturally infested and not infested (protected) control plots. The experiment was performed in isolated conditions in two field cages covered by nets. The aphids overwintered on wheat and got into cage, extremely quickly multiplied, therefore there was no need to apply any artificial aphid infestation. Highly significant differences were demonstrated among genotypes in infestation severity of R. padi as well as in losses of grain yield and thousand-kernel mass. The most resistant variety ‘GK Zombor’ had 25% infestation, and the most susceptible one ‘GK Lili’ had 79.2%. The reduction of grain yield of the most tolerant genotypes (‘GK Korány’, ‘Downy’, ‘Mv 4’, ‘Jubilejnaja 50’, ‘Mv 8’, ‘GK Kincsö’ and ‘GK Zombor’) was 26–33%, and that of thousand-kernel mass was 23–30%. The most sensitive genotypes (‘GK Lili’, ‘GK Örzse’, ‘GK Koppány’ and ‘Mv 13’) suffered 58–63% losses in yield, and 40–50% in thousand-kernel mass. A close correlation was found between infestation of R. padi in different wheat genotypes and losses of grain yield (r=0.7572, P〈0.001). Also there were tolerance differences among genotypes even within the same level of infestation. The reductions of thousand-kernel mass correlated very closely with the reductions of grain yield (r=0.9212, P〈0.001), that makes screening possible by reductions of thousand-kernel mass. These results have found application in breeding. The leaf pubescence of the varieties studied did not generally influence the infestation by R. padi.
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  • 26
    ISSN: 1573-5060
    Keywords: mediterranean stress ; small grain cereals ; susceptibility index ; yield ; Triticum aestivum ; Triticum durum ; Hordeum vulgare ; bread wheat ; durum wheat ; barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Among the cultivars of bread wheat, durum wheat and barley grown in the South of Italy, genetic variation for adaptation to the high temperature and drought stress conditions typical of the Mediterranean environment has been found. The basic data have been extrapolated from 5 years of Italian national network cultivar trials, where 20–30 cultivars were grown in replicated plot trials in 30–50 locations per year, including some where stress strongly affected grain yield. After careful identification of the most representative years and testing sites it was possible to characterise the cultivars on the basis of the grain yield in stress conditions and the Fischer & Maurer (1978) susceptibility index and to find genotypic differences sufficiently repeatable in years. The cultivars giving the best yield under stress associated with low susceptibility indices were in bread wheat: Etruria, Spada, Pandas, Centauro, Oderzo, Costantino and Gladio, in durum wheat: Aldura, Arcangelo, Adamello, Vespro and Capeiti, in barley: Fleuret, Barberousse, Jaidor, Express, Trebbia, Georgie, Dahlia, Criter and Magie.
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  • 27
    ISSN: 1573-5060
    Keywords: common wheat ; Triticum aestivum ; resistance genes ; Erysiphe graminis tritici ; powdery mildew ; monosomic analysis ; allelism tests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Several wheat cultivars/lines were inoculated with isolates of Erysiphe graminis tritici to identify new genes/alleles for resistance. The wheats were tested with 13 isolates that had been characterized from responses on differential lines with known resistance genes. Gene Mlk which occurs in cultivars ‘Kolibri’, ‘Syros’, ‘Ralle’ and several other European common wheats was found to be an allele at the Pm3 locus and is now designated Pm3d. The mildew resistance in an old Australian wheat, ‘W150’, is conferred by a single gene also allelic to Pm3 and now designated Pm3e. The near-isogenic line ‘Michigan Amber/8*Cc’ possesses another allele now designated Pm3f. A Syrian land variety of common wheat shows mildew resistance that is conditioned by the combination of genes Pm1 and Pm3a. Finally, two accessions of Triticum aestivum ssp. sphaerococcum appeared to possess the Pm3c allele.
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  • 28
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    Euphytica 70 (1993), S. 35-42 
    ISSN: 1573-5060
    Keywords: biomass ; heritability ; response to selection ; selection ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Biomass (above ground plant parts) yield may be a useful selection trait for yield improvement in wheat (Triticum aestivum L.). This study was conducted to estimate realized heritability of biomass yield and to determine the response to selection for high and low biomass yield in 8 genetically diverse populations of spring wheat under two production systems. Selections were made among the F3 lines. Progenies of the selected lines were evaluated in replicated field tests in the F4 generation under high fertility and low fertility production systems at Rampur, Nepal, in 1991. Fertility level had a significant effect on biomass yield, grain yield, effective tiller number, number of kernels per spike, thousand kernel weight, and harvest index. Selection in the F3 for high and low biomass yield was effective in identifying F4 lines with high and low biomass yield, respectively. Biomass yield differences between high and low selection groups in the F4 generation, expressed as percent of the mean of the low selection group and averaged over the eight populations, were 53.9 and 36.5% higher than the mean of the low selection group under the high and the low fertility production systems, respectively. The corresponding figures for grain yield were 48.8 and 34.9% under the high and the low production systems, respectively. Also, selection for high biomass yield resulted in higher effective tiller number, and number of kernels per spike, but lower harvest index. Realized heritability estimates for biomass yield were greater at high fertility (range 0.49 to 0.85) than at low fertility (range 0.22 to 0.44). Biomass yield showed positive genotypic correlations with grain yield, effective tiller number, and number of kernels per spike but a negative correlation with harvest index. The results indicated that selection for high biomass yield should bring about positive improvements in biomass yield, grain yield, effective tiller number, and number of kernels per spike. The correlation between F3 and F4 generations suggested that biomass yield in the F3 generation was a good predictor of biomass yield and grain yield in the F4 generation. Selection for biomass yield in wheat should be made under the standard production system to obtain a realistic response.
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  • 29
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    Euphytica 72 (1993), S. 1-7 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia striiformis ; yellow rust ; stripe rust ; genetics ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nine Mexican spring bread wheat (Triticum aestivum L.) cultivars derived from CIMMYT germplasm and the U.S. spring wheat cultivar Wheaton were susceptible to the Mexican Puccinia striiformis pathotype 14E14 in seedling growth stage, but displayed different levels of adult plant resistances to the same pathotype when tested in the field. One hundred and eighteen random F2 plant derived F3 and F5 lines from the crosses of these ten adult plant resistant wheats and susceptible cultivar Jupateco 73S were evaluated in the field. The moderate adult plant resistance of Penjamo 62, Lerma Rojo 64, Nacozari 76, Tesia 79, and Wheaton was under monogenic genetic control and was attributed to the adult plant stripe rust resistance gene Yr18. The moderate resistances of Cleopatra 74, Zaragoza 75, and Apache 81 were also monogenic, but gene Yr18 was absent. Pavon 76 carried two partially effective additive genes; and the adult plant resistance of Tonichi 81 was based on additive interaction involving Yr18 and two additional partially effective genes. Tonichi 81 does not carry any seedling resistance gene, however, the adult plant resistance is highly effective worldwide. This resistance, designated as the Yr18 complex, is of a durable nature. The partial adult plant resistance of Pavon 76 has also remained durable in Mexico and other countries where it is grown.
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  • 30
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    Euphytica 72 (1993), S. 107-113 
    ISSN: 1573-5060
    Keywords: wheat ; Triticum aestivum ; recurrent selection ; resistance to scab ; gene pool ; Taigu male-sterile gene Ms2 ; breeding method ; Gibberella zeae ; Fusarium graminearum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Scab caused by Gibberella zeae Petch., in common wheat, is one of the most severe diseases in China. A source population C0, bred for scab resistance, was developed through three cycles of multiple-parent crossing and intercrossing by means of the dominant male-sterile gene Ta1 (Ms2), according to Wu's scheme. Phenotypic recurrent selection methods for increasing the resistance to scab-infection of spikelets and seeds with the male-sterile plants were carried out simultaneously in Nanjing and Shanghai and at Jianyang, Fujian Province, for three cycles. The generations from C0 to C3 and two check cultivars were evaluated, using a randomized block design, under conditions of an artificially induced epidemic of scab during 1988–1990. The results indicate that there were significant differences in the resistance to scab between these generations. On average, the percentages of diseased spikelets and seeds of the male-fertile plants were reduced by 9% and 10%, respectively. The frequency of resistant plants was distinctly enhanced by recurrent selection. Analysis of variance showed that no significant differences existed between cycles of recurrent selection in agronomic characters such as plant height, spikes per plant, spike length, numbers of spikelets and seeds per spike, weight of seeds per spike and 100-kernel weight, days to heading and to maturity. Except for plant height, most of these traits tended to be slightly improved with improvement of resistance in the gene pool. The variance for resistance in the generations was decreased under selection. Recurrent selection for scab resistance using the dominant male-sterile gene Ta1 (Ms2) was both an effective and feasible breeding method for producing this character in wheat.
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  • 31
    ISSN: 1573-5060
    Keywords: wheat ; Triticum aestivum ; resistance selection ; disease complexes ; pathogen mixtures ; septoria nodorum blotch ; Leptosphaeria nodorum ; septoria tritici blotch ; Mycosphaerella graminicola ; yellow spot ; tan spot ; Drechslera tritici-repentis ; Pyrenophora tritici-repentis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In controlled inoculation studies with Septoria nodorum and Pyrenophora tritici-repentis, estimates of the relative proportion of each pathogen demonstrated differences in the responses of cultivars to pathogen mixtures that were not apparent from measurements of diseased leaf areas. Under field conditions estimates of the relative proportion of S. nodorum, P. tritici-repentis and S. tritici varied between field screening locations in Western Australian but also between lines within locations. Lines with known resistance to P. tritici-repentis and S. tritici, but susceptible to S. nodorum, could not be distinguished from susceptible lines on the basis of leaf area diseased or grain weight depression when S. nodorum was present in the disease complex. Such conditions, while suitable for the selection of combined resistance to these pathogens, were unsuitable for identifying resistance to individual pathogens. As symptoms were similar, the proportion of diseased leaf area sporulating with each pathogen provided a means of measuring the variation in disease development induced on lines varying in resistance. Knowledge of the components of disease and their relative importance were essential in understanding varietal response information under mixed infections of these leaf spot pathogens.
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  • 32
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    Euphytica 72 (1993), S. 171-175 
    ISSN: 1573-5060
    Keywords: amylose content ; flour peak viscosity ; Japanese noodle ; Triticum aestivum ; Wx protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To characterize superior genotypes for the white Japanese noodle, endosperm starch properties including amylose content, flour peak viscosity and starch-granule bound Waxy (Wx) proteins were compared using several cultivars preferred for noodle manufacture. Amylose contents from three seasons trials and flour peak viscosity from two seasons trials varied among cultivars. Low amylose content was a common property in the noodle cultivars, whereas a high peak viscosity was not always the case. When the Wx proteins were analyzed by sodium dodecylsulfate polyacrylamide gel electrophoresis, a clear reduction in the amount of low molecular weight protein or a lack of the high molecular weight protein occurred in the noodle cultivars. Segregation of Wx proteins was detectable in a B1F1 population, indicating that the Wx protein analysis has a potential as a surrogate of selecting low amylose genotypes in early generations.
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  • 33
    ISSN: 1573-5060
    Keywords: intergeneric introgression ; Oryza sativa, prolamins ; RFLP ; Triticum aestivum ; X Oryticum oryzoides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The DNA of a putative rice x wheat hybridization derivative (X Oryticum oryzoides) from China, the DNA of its parental rice cultivar and the DNA of a wheat line were digested with ten different restriction endonucleases, resolved by agarose electrophoresis, Southern blotted and hybridized using genomic wheat DNA as a probe. Phenol extracted, ethanol and cetyltrimethylammonium bromide precipitated DNA of the putative hybrid showed a restriction fragment length polymorphism (RFLP) different from that of the parental rice. When the DNA was further purified by Qiagen chromatography, the RFLP differences were not detected. Hence the apparent RFLP differences were probably due partial digestion of the less pure DNA preparations by the restriction endonucleases. No real introgressed fragments from wheat genome could be shown. The HpaII/MspI sites were more frequently digested with MspI than with HpaII in rice and hybridization derivative DNA, but the sites were evidently more frequently methylated in wheat DNA. Thus, in terms of methylation of the DNA, the hybridization derivative was much more like the rice parent than the wheat parent. The hybridization derivative showed a single endospermal protein (mass 19 kg mol-1) not detected in the parental rice cultivar. This minor protein was soluble in buffered 50% isopropanol and precipitable with methanol. The results indicate that there are no or only short introgressed sequences from wheat in the rice/wheat derivative, a result which might be considered in breeding efforts with the hybrid derivative.
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  • 34
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    Euphytica 72 (1993), S. 197-203 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; high temperature tolerance ; yield component ; selection criteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Agronomic and yield data were collected from two trials each containing 16 bread wheat genotypes, planted two years under late sowing conditions of high temperature (above 30° C) and one year under a normal sowing time environment. The aim was to study the character response and yield correlations with yield components and other characters under high temperature conditions with full irrigation. The results show that yield, seeds per spike, biomass, and plant height are more thermo-sensitive than spike number per square meter, 1000 kernel weight, and test weight. The grain-filling rate was more temperature-sensitive than days to anthesis and duration of grain-filling. Simple phenotypic correlation analysis indicated that yield was highly and positively correlated with seeds per spike, biomass, and harvest index (HI), independent of seasons and genotypes under high temperatures. The seeds per spike accounted for variation of yield ranging from 35.2 to 78.1%. Effect of earliness on the yield under high temperature was highly dependent on the temperature regime during the heading stage. Grains per spike, biomass, HI, and test weight could be considered potential selection criteria for yield under high temperature. Analysis of yields under normal and late sowing conditions failed to reveal any association between the yield potential in normal sowing date and the performance of varieties under high temperature.
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  • 35
    ISSN: 1573-5060
    Keywords: bread wheat ; combining ability ; inheritance ; Karnal bunt ; resistance ; Tilletia indica ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An analyis of an F1-based incomplete diallel was conducted involving 11 parents with different levels of resistance to Karnal bunt (Tilletia indica (Mitra)). It demonstrated that general combining ability (GCA) and thus additive or additive × additive gene effects were very important in the inheritance of resistance, accounting for 86.9% of the variation. Further analysis concentrated on F3 lines derived from individual random F2 plants from crosses with resistant varieties having the highest negative GCA effects. It was shown that the varieties Weaver and W499 have single dominant genes of resistance, which are different from each other, and which differ from a single allelic gene in varieties K342 and Cruz Alta. The majority of the crosses did not demonstrate a relationship between Karnal bunt infection and the number of days to heading. Resistant F3 lines varied in the number of days to heading from 80 to 100.
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  • 36
    ISSN: 1573-5060
    Keywords: Russian wheat aphid ; Diuraphis noxia ; wheat ; Triticum aestivum ; monosomic analysis ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Russian wheat aphid, Diuraphis noxia (Mordvilko) (Homoptera: Aphididae), has become an important pest of wheat (Triticum aestivum L.) in the United States. The aphid causes a phytotoxemic reaction in wheat evidenced by local and systemic chlorosis and rolling of infested leaves. Developing resistance in wheat cultivars to D. noxia is an essential factor in controlling the damage caused by this pest. Several sources of genetic resistance to D. noxia have been identified in wheat germplasm. Monosomic analysis of the monogenic resistant T. aestivum accession PI137739 has shown that the gene (Dn1) for resistance is carried on chromosome 7D. It appears that chromosome 7B may carry a second resistance gene for D. noxia that might be a source of minor or complementary gene action for resistance.
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  • 37
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    Plant and soil 155-156 (1993), S. 155-158 
    ISSN: 1573-5036
    Keywords: nitrate uptake ; root activity ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A technique was developed to determine the physiological activity of defined sections of seminal roots of wheat grown in sand. Wheat plants were grown for 2 weeks in narrow columns of N-deficient sand to which all other nutrients had been added. The columns were split longitudinally and 15N-labelled nitrate, in an agar medium, supplied to 2 cm sections of root. Shoots and roots were analysed after 24 h to determine the uptake of 15N. Three sections were examined on either the secondary or tertiary seminal root: 1 cm from the seed (basal segment), 35 cm from the seed (middle segment) and 4 cm from the root apex (apical segment). Total uptake was greatest from the basal and middle segments, declining by 50% from the apical segment. However, uptake per unit root length, including exposed sections of lateral roots, was not significantly different along the root.
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  • 38
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    Plant and soil 155-156 (1993), S. 293-296 
    ISSN: 1573-5036
    Keywords: efficiency ; genotypic differences ; phosphorus ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In an attempt to evaluate whether breeding and selection for high yielding capacity did change the P requirements of modern wheat cultivars, the response of two wheat cultivars to different levels of P supply was investigated. A traditional cultivar ("Peragis") and a modern cultivar ("Cosir") were cultivated in a C-loess low in available P and high in CaCO3 in 120 cm high PVC pots. Shoot and root growth at different developmental stages was compared. The grain yield of the modern cultivar Cosir was higher at limiting and non-limiting P supply and, therefore, this cultivar can be considered as more P-efficient than the traditional cultivar. From the results it can be concluded that the main factors contributing to the higher P efficiency of the modern cultivar are (i) efficient use of assimilates for root growth characteristics which enhance P acquisition: smaller root diameter, and longer root hairs, (ii) efficient remobilization of P from vegetative plant organs to the grains, and (iii) lower P requirement for grain yield formation because of lower ear number per plant but higher grain number per ear.
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  • 39
    ISSN: 1573-1561
    Keywords: Parthenium hysterophorus ; Eichhornia crassipes ; Triticum aestivum ; biomass ; inhibitory activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The relative effect of residue of leaf, flower, stem, and root of parthenium (Parthenium hysterophorus L.) on growth of water hyacinth was studied. The inhibitory activity of the residue as shown by its effect on biomass and healthy leaf number (HLN) of treated plants was in the order: leaf and flower 〉stem 〉root. Total phenolic acids in the medium after 72 hr of suspending the plant part residue were maximum in flower followed by leaf, root, and stem, successively. The dry leaf powder (DLP) and dry flower powder (DFP) at and above 0.50% (w/v) and dry stem powder (DSP) at 1.00% (w/v) killed water hyacinth in about one month. Dry root powder (DRP) at the highest dose (1.25% w/v) reduced the growth of the treated plants drastically, but the plants recovered after about one month. The DSP at 0.50% (w/v) and DRP at 0.25–0.75% (w/v) supported growth of treated plants, probably due to lower levels of inhibitors, allowing utilization of constituents of the residue as nutrients. Using wheat seedlings as a reference material, it was observed that in aquaculture at different levels of parthenium plant parts residue, water hyacinth plants were much more sensitive to inhibitory activity. Thus, water hyacinth is suggested as a material for bioassay of inhibitory activity of the parthenium plant residue.
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  • 40
    ISSN: 1573-5060
    Keywords: wheat ; Puccinia recondita f.sp. tritici ; Triticum aestivum ; slow rusting resistance ; leaf rust ; brown rust ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Forty F6 lines, the two parental lines, and a susceptible check cultivar of wheat (Triticum aestivum L.) were inoculated in the young flag leaf stage with leaf rust (Puccinia recondita f.sp. tritici) and evaluated for latent period, receptivity, and uredinium size in a greenhouse experiment. Genotypic (rg) and phenotypic (rp) correlations between latent period and uredinium size were −0.81 and −0.62, respectively. A negative correlation (rg=−0.50, rp=−0.41) was found between latent period and receptivity and a positive correlation (rg=0.28, rp=0.26) between uredinium size and receptivity was found. Area under the disease progress curve (AUDPC) and final rust severity (FRS) obtained from a subsequent field study with common entries were negatively correlated with latent period and positively correlated with uredinium size. Correlations of receptivity with both AUDPC and FRS were not significant. The distributions of F6 family mean uredinia size and latent period were continuous between slow rusting and fast rusting parents: however, the distribution for receptivity was discrete. Narrow-sense heritability estimates were 63%, 57%, and 47% for uredinium size, latent period, and receptivity, respectively. Estimates of the minimum number of effective factors were three for latent period and three or four for the uredinium size and receptivity. The components are controlled by closely linked genes or due to pleotropic effects of the same gene.
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  • 41
    ISSN: 1573-5060
    Keywords: A genome ; C-banding ; meiosis ; Triticum aestivum ; Triticum monococcum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The meiotic pairing behaviour at metaphase I of a Triticum aestivum×Triticum monococcum hybrid has been studied by means of the C-banding technique to ascertain the homology between the chromosomes in the A genome of the two species. The technique allowed the A and B genome chromosomes and the 2D, 3D and 5D chromosomes to be identified. Differences in the level of chromosome pairing in the A genome were noted. The T. monococcum 4A chromosome did not pair with any of the T. aestivum chromosomes in any of the metaphase I cells analysed. Two reciprocal translocations between the 2B and 2D chromosomes on one side and the 2A and 3D on the other side have been identified. The usefulness of the C-banding technique in the study of chromosome homology among species related to wheat is discussed.
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  • 42
    ISSN: 1573-5060
    Keywords: barley ; Hordeum vulgare ; grain yield ; plot size ; variety trials ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A coordinated series of experiments aimed to estimate border effect and intergenotypic competition was carried out on bread wheat and barley in Italy and Germany. The performance of the varieties grown in alternate rows 14–17 cm apart proved to be strictly dependent on the bordering variety. The varieties grown in adjacent plots consisting of eight rows showed significant border effects: the two external rows yielded up to 40% more than the two innermost, and the two 30 cm end hills facing the alleys yielded 29% more than the central part of the plot. Is has been suggested that in variety trials the first 30–40 cm at both ends of each plot should be removed mechanically. In spite of the border effect detected in the outer rows of the plots, the varieties similarly exploited the extra space available at the borders, and the space of 30 cm left between adjacent plots seems to be sufficient to dilute the intergenotypic competition at plot level to an extent only marginally affecting variety performance. In conclusion, grain yield based on whole plot (8 rows, 6–10 m2) appears to provide a good estimate of ‘true yield’, providing that it is based on the actual area of the plot, including the uncropped strip between adjacent plots. By contrast, the reduction of plot size to 1–3 rows is not feasible without introducing considerable bias into the estimation of the yield potential of the varieties.
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  • 43
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    Euphytica 69 (1993), S. 89-94 
    ISSN: 1573-5060
    Keywords: electrophoresis ; glutenins ; alleles ; sedimentation value ; wheat ; quality score ; high molecular weight glutenins ; Triticum aestivum ; Yugoslavia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The high molecular weight (HMW) subunits of glutenin extracted from flour of 36 Yogoslav wheat cultivars were separated by SDS-PAGE to identify their alleles, and the frequency of each allele was calculated. Eleven alleles from the three Glu-loci were recognized, three at the Glu-A1 locus, six at the Glu-B1 locus and two at the Glu-D1 locus. The most frequent allele was “a” (55.5%) from Glu-D1, which controls synthesis of subunits 2+12. The Glu-1 quality score varied from 4 (KG-III/27, KG-75, Morava and KG-101/7) to 10 (Lepenica). The mean Glu-1 quality score of cultivars and lines from Kragujevac was 6.8, for cultivars from Zagreb 7.2, and for cultivars from Novi Sad was 7.9. Most of the genotypes with a quality score of 8 or above, had high sedimentation values (Zeleny test). There were no significant differences in allelic composition at the Glu-1 loci among wheat genotypes from Kragujevac, Novi Sad and Zagreb.
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  • 44
    ISSN: 1573-5060
    Keywords: X Triticosecale ; triticale ; Triticum aestivum ; wheat ; Secale cereale ; rye ; Fusarium spp ; pathogenicity ; head blight ; seedling resistance ; adult plant resistance ; grain yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Pathogenicity of 20 isolates of 12 Fusarium species recovered from triticale seed against seedlings of 14 varieties of winter cereals (triticale, wheat, and rye) was tested. The most pathogenic inoculum was a mixture of isolates (a composite isolate) of all the species. The following species were individually the most pathogenic: F. avenaceum, F. culmorum, F. sambucinum var. coeruleum, and F. graminearum. Winter triticale was more resistant to seedling blight than rye but more susceptible than wheat. Also reactions of 31 winter and 12 spring varieties of cereals to head inoculation with a composite isolate of 4 Fusarium spp. (F. avenaceum, F. culmorum, F. graminearum, and F. sambucinum var. coeruleum) was studied. In comparison to other cereals of similar type winter and spring wheat appeared to be the most susceptible while winter rye reaction was comparable to winter triticale. Spring and winter triticale varieties responded to head infection intermediately. There was no significant correlation between seedling and head reactions to infection with Fusarium spp. for winter rye and triticale. For winter wheat a negative trend was found. The above findings imply that screening of cereals at the seedling stage can not be used to predict the resistance to head blight. Nevertheless, resistance at the stage is highly desirable to prevent excessive damage of the crops due to the seedling blight incited by Fusarium spp..
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  • 45
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    Euphytica 71 (1993), S. 107-113 
    ISSN: 1573-5060
    Keywords: Puccinia striiformis ; resistance genes ; stripe rust ; Triticum aestivum ; wheat ; yellow rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Carstens V, one of the wheat cultivars used to differentiate races of Puccinia striiformis (stripe rust or yellow rust) in Europe, was crossed with stripe rust susceptible cultivars and cultivars with genes for resistance to the disease to determine the genetic basis of its stripe rust resistance. Seedlings of the parents and F1, F2, and F3 progeny were evaluated for resistance to North American races of P. striiformis. Based on those evaluations, Carstens V has three genes for resistance to North American race CDL-21, two genes for resistance to races CDL-17, CDL-20, and CDL-29, and one gene for resistance to race CDL-27. The genes that confer resistance to race CDL-17 also confer resistance to race CDL-21. The three genes were either dominant or recessive depending upon the race used in the test and the cultivar used in the cross. None of the genes in Carstens V are the same as the genes for resistance in Cappelle Desprez, Chinese 166, Clement, Compair, Heines Peko, Hybrid 46, Minister, Nord Desprez, Triticum spelta album, and Vilmorin 23.
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  • 46
    ISSN: 1573-5060
    Keywords: pattern analysis ; genotype x environment interaction ; grain yield ; mega-environments ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The International Spring Wheat Yield Nursery (ISWYN) has been distributed annually since 1964 and the results provide a base for investigating relationships among locations. Ordination and clustering of locations was conducted using 26 years of grain yield data. Ordination and clusters based on the discrimination of germplasm were compared with ‘mega-environments’, which are groupings of locations defined by CIMMYT on the basis of climatic factors and perceptions of major biotic and abiotic stresses. Discrepancies among mega-environmental groupings, clusters and ordinations may identify locations for which major stresses affecting wheat yield are yet unidentified. Major environmental discriminators were latitude and the presence or absence of stress, although there was little association of locations due to limited moisture availability. We identified two major spring wheat environments, typified as Asian and European, and suggest the mega-environmental classification does not explain all significant associations among locations. Location groupings based on discrimination of germplasm should be considered in parallel to mega-environments on a regular basis and we propose breeding for a base of broadly adapted germplasm to which specific stress tolerances are incorporated.
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  • 47
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    Euphytica 74 (1993), S. 9-17 
    ISSN: 1573-5060
    Keywords: compensation ; competition ; height ; Triticum aestivum ; winter wheat ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The relationship between plant height, competitive ability and yield performance was examined for six cultivars of winter wheat (Triticum aestivum L. em. Thell.) in southern Alberta. Competition significantly reduced grain yield, heads.m-2 and kernels.m-2. Competition also reduced heads.plant-1 and kernels.head-1 but did not affect plants.m-2 or kernel weight. For yield and other area-based yield components, multiple regression showed that interference by competitors was increased by reducing the height of the neighbouring cultivar and by increasing both competitor height and the ratio of competitor height to cultivar height. Intensities of yield interference in pure stands of the six cultivars were predicted by substituting their height into this multiple regression model. These predictions showed a one-to-one relationship (b=1.01; p〈0.01; R2=0.85) with pure stand grain yields, indicating that reduced inter-plant interference is a yield-positive trait that is inevitably present in high yielding cultivars. For plant-based yield components (heads.plant-1, kernels.head-1 and kernel weight), cultivar-specific compensation between traits maintained a balance between the number and the size of heads and kept kernel weight constant. These responses to optimize the development of viable kernels modified or obscured the effect of competition.
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  • 48
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    Plant and soil 155-156 (1993), S. 457-459 
    ISSN: 1573-5036
    Keywords: copper ; development ; leaf emergence ; nutrient deficiency ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Copper deficiency can delay flowering and plant maturity. However, the effect of copper deficiency on the rate of leaf emergence has not been quantified. We tested the hypothesis that low copper supply decreases the rate of leaf emergence of wheat (Triticum aestivum L. cv Gamenya). Copper foliar sprays are commonly applied to wheat. We examined the response of the rate of leaf emergence to a foliar application of copper sulphate. Wheat was grown in root cooling tanks (20°C) in the glasshouse. Soil copper treatments were applied as solutions of CuSO4.5H2O at three rates: Cu0=no added Cu, Cu400=400 μg Cu per 3 kg pot, and Cu1600=1600 μg Cu per pot. An additional treatment of a foliar spray of CuSO4.5H2O (0.4 mg Cu per plant) was applied to Cu0 and Cu400 plants 45 days after sowing (5.5 leaves on the main stem). Leaves on the main stem were counted and the rate of leaf emergence was estimated from the regression of number of emerged leaves against thermal time (base 0°C). The phyllochron was calculated as 1/rate of emergence. Leaves on Cu0 and Cu400 plants took longer to emerge than on Cu1600 plants, with the phyllochron of Cu1600 plants being 130 compared to 137 for the Cu400 plants and 158 for the Cu0 plants. The foliar application of CuSO4 at the 5–6 leaf stage resulted in a decrease in the phyllochron of the Cu0 plants to 127, but no change in that of the Cu400 plants.
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  • 49
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    Plant cell, tissue and organ culture 35 (1993), S. 59-67 
    ISSN: 1573-5044
    Keywords: amino acids ; anther culture ; culture medium ; maltose ; bread wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Different media were evaluated with anthers of five spring wheat (Triticum aestivum L.) genotypes for their ability to produce embryos and green plants in anther culture. Our first experiment showed that the addition of a combination of 19 amino acids significantly increased the number of embryos and green plants obtained. The mean number of green plants per 100 anthers for the two genotypes in this experiment, HY320 and B723, went from 28.2 without amino acids in the medium, to 46.7 with addition of amino acids. Our second experiment with the genotypes HY320, Wim and Laval-19 showed that liquid medium with Ficoll is more efficient for anther culture (9.9 green plants/100 anthers) than solid (0 green plants), gelationous media (2.5 green plants/100 anthers) or liquid medium with Membrane Rafts (0 green plants; Hoechst Celanese Corp.). Our third experiment revealed that the effect of replacement of sucrose by maltose varied with the genotype of the donor plant. Maltose partially inhibited the androgenesis of three responsive genotypes, HY320, Wim and Reliance (40.3 green plants/100 anthers instead of 43.9 with sucrose), while maltose significantly increased the androgenesis of the recalcitrant genotype Laval-19 (10.8 green plants/100 anthers instead of 5.4 with sucrose). An amino acid x maltose interaction was also observed. Amino acids without maltose increased androgenesis, but the addition of maltose to the amino acid-enriched medium eliminated this positive effect of the amino acids.
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  • 50
    ISSN: 1573-5060
    Keywords: China ; crossability ; landrace ; rye ; Secale cereale ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The crossability percentages of 118 landraces of wheat (Triticum aestivum L.) from Shaanxi and Henan provinces, China with rye (Secale cereale L.) have been tested. 14 landraces possess a higher crossability percentage than Chinese Spring, 30 landraces have a similar to and 74 landraces have a lower crossability percentage than Chinese Spring or are non-crossable with rye. Most landraces with high crossability percentage occur in south part of Shaanxi, and the west and southwest of Henan, their geographical distribution in these regions fits up with that in the Sichuan province.
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  • 51
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    Euphytica 68 (1993), S. 33-41 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia graminis tritici ; stem rust ; near-isogenic lines ; linkage drag
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two sets of near-isogenic lines of wheat carrying single genes for stem rust resistance were grown in yield tests to determine whether the resistance genes were deleterious. One set was based on the cultivar Marquis and the second set on a susceptible, day-length insensitive line, LMPG. The results indicated that the effects of resistance genes vary with different genes and different environments. However, there appeared to be a tendency for resistance genes to reduce yield. In most cases the reductions were too small to be of much concern to wheat breeders.
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  • 52
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; glutenin sub-units ; bread-making quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The composition of the high-molecular-weight (HMW) subunits of glutenin encoded by Glu-1 in hexaploid wheat (Triticum aestivum L.) was determined for 99 varieties bred in China. Seventy-nine varieties were homogeneous for HMW glutenin subunit composition. The majority of the remainder were heterogeneous at one Glu-1 locus, with two varieties heterogeneous at two loci. One variety carried a pattern of subunits not encountered in previous studies. The majority of varieties, as in previous studies, carried subunits 7+8 or 7+9 encoded by Glu-B1. In contrast, there was a relatively high occurrence of subunits 2+12 at Glu-D1. It was shown that a high proportion, 69%, of the potential variation in bread-making quality, as assessed by SDS-sedimentation volume, was accounted for by variation in the composition of the HMW subunits.
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  • 53
    ISSN: 1573-5060
    Keywords: Wheat ; Triticum aestivum ; landrace ; semidwarf ; phosphorus ; nitrogen ; fertilization ; genetic improvement ; breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In recent decades, most winter wheat (Triticum aestivum L.) breeding in the United States has been done in field nurseries in which the soil receives ample fertilization. To determine the effects of these breeding efforts on productivity under low-fertility conditions, we evaluated twenty-nine winter wheat genotypes (seven Asian landraces; thirteen standard-height U.S. cultivars released between 1874 and 1971; and nine semidwarf cultivars released between 1977 and 1988) under severe fertility stress at three Kansas, USA locations. Experiments included fertilized and unfertilized treatments. The modern, semidwarf cultivars yielded 18% and 20% more, on average, than landraces and standard-height cultivars under low and high fertility, respectively; however, only the latter difference reached the 5% significance level. At only one location (Hays) was there a significant genotype X fertility interaction: there, 89% of the semidwarf cultivars, only 8% of the standard cultivars, and 57% of the landraces responded to fertilization. The regression coefficient of mean grain yield (unfertilized) on year of introduction or release for standard and semidwarf cultivars was zero, indicating that a century of breeding has produced no genetic improvement in performance under these low-fertility conditions. Although we found that the usual yield advantage of modern cultivars is not expressed under very low fertility, we saw no evidence that older cultivars are superior under those conditions.
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  • 54
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    Euphytica 70 (1993), S. 127-129 
    ISSN: 1573-5060
    Keywords: China ; crossability ; landrace ; rye ; Secale cereale ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The crossability percentages of 282 accessions of wheat (Triticum aestivum L.) collected in Tibet, China with rye (Secale cereale L.) have been tested. Five collections have a similar to and 277 accessions have a lower crossability percentage than Chinese Spring or are non-crossable with rye. The accessions with high crossability percentage occur along the highway near Lhasa. No landraces with higher crossability than Chinese Spring and rare landraces with similar crossability to Chinese Spring indicated that the landraces in Tibet region are different from those in Sichuan, Shaanxi and Henan provinces in the distribution frequency of high crossability, and there is no distribution of recessive kr4 alleles.
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  • 55
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    Euphytica 70 (1993), S. 131-140 
    ISSN: 1573-5060
    Keywords: adult plant resistance (APR) ; area under disease progress curve (AUDPC) ; infection frequency (IF) ; latency period (LP) ; partial resistance (PR) ; P. recondita f.sp. tritici ; sporulating leaf area (SLA) ; Triticum aestivum ; wheat ; wheat leaf rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Winter wheat genotypes were tested for resistance in the field by assessing the percentage sporulating leaf area after infection with wheat leaf rust. The disease level in the first field trial was too low for selection. In the second field trial a low sporulating leaf area was found on several genotypes showing a susceptible infection type. These genotypes possibly have partial resistance. Six genotypes possibly possess adult plant resistance, as they showed a resistant infection type and a low sporulating leaf area. The latency period, infection frequency and uredosorus size of sixteen genotypes were determined in the greenhouse after infection with two races of leaf rust at two temperature regimes. The temperature × genotype interaction, found for latency period and infection frequency, was mostly influenced by the cultivars Cerco, Tundra and Miller. Adult plant resistance was postulated for four genotypes whereas another four appeared to have partial resistance. Only one of the sixteen genotypes (Apexal) possessed adult plant resistance and two genotypes (Arminda and Cappelle Desprez) showed partial resistance in the field as well as in the greenhouse.
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  • 56
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia striiformis ; yellow rust ; interplot interference ; quantitative resistance ; representational error ; airborne pathogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In small plots, adjacent to one another, a representational error can be expected when screening for quantitative forms of resistance to airborne pathogens. The representational error or interplot interference may occur as an underestimation of the level of resistance and/or as an error in the ranking of the entries tested. Four experiments were carried out with wheat (Triticum aestivum) exposed to yellow rust (Puccinia striiformis), three in Kenya, one in Mexico (exp. III). In experiment I 57 entries were compared in an unreplicated trial with three adjacent and one isolated plot situation. The range in the standard deviation of and the ranking order for disease severity (DS) between the 57 entries were the same for all plot situations at all observation dates. In experiment II nine entries from experiment I, representing a wide range of quantitative resistance and having a similar heading date, were compared in adjacent plots consisting of two rows of 10 m in eight replicates and isolated plots of one, six and ten rows of 4 m in three replicates. The range in the standard deviation of and the ranking order for DS between the entries were very similar for the four plot situations. The 10 entries in experiment III differed from those in experiment II, but represented a similar wide range of resistance. Three adjacent plot situations of 0.9×0.5 m, 0.9×2.0 m and 2.7×2.0 m, respectively, were compared with one isolated one with plots of 2.7×2.0 m. The ranking order was not affected, the range in and the standard deviation of the DS in the isolated plots were slightly larger than in the adjacent plots. In experiment IV two mixtures of two entries each were made. Per mixture one entry was fairly resistant (R) the other rather susceptible (S). Within each mixture the entries had a similar heading date. The ratios of the R:S mixtures were 0:100, 50:50, 67:33, 75:25, 82:18 and 100:0. The DS of each entry was the same as its DS in monoculture irrespective of the mixture ratio. There was no mixture effect on DS. The three experiments in Kenya gave no indication of any interplot interference occurring. In Mexico there was a very slight underestimation of the resistance in adjacent plots. The ranking order was always the same irrespective of the test plot situation. The screening of wheat for yellow rust resistance in small adjacent plots is representative for the farmers fields. This is contrary to what has been found in other windborne pathosystems such as barley-barley leaf rust (strong under estimation of resistance), barley-powdery mildew (some under estimation of resistance and different ranking order) and durum wheat-stem rust (fair under estimation of resistance).
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  • 57
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    Euphytica 73 (1993), S. 241-254 
    ISSN: 1573-5060
    Keywords: breeding populations ; ear:stem ratio ; grain yield ; harvest index ; heritability ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Earlier studies showed that the ratio of the weight of the wheat ear to stem at anthesis (ear:stem ratio) may give a better indication of potential yield than harvest index because it is determined early in the life cycle and is not affected by post anthesis stress. These studies concluded that selection for high ear:stem ratio at anthesis may lead to further improvement in grain yield of wheat. The present work was undertaken in the field to identify lines varying in ear:stem ratio in breeding populations and to study its implications for yield improvement. At anthesis stem length, ear length, tiller number, dry weight of stem and ear and ear:stem ratio were measured in 14 crosses on F2 single plants and F2 derived lines grown in the F3, F4, and F5 at three locations in Western Australia over four seasons. In addition, biomass, grain yield and yield components were measured on selected crosses at two locations on F2 derived lines grown in the F4 and F5. There was a considerable range of ear:stem ratio between and within the crosses studied. Although ear:stem ratio was strongly correlated with stem length, there was substantial variation within stem length classes. Ear:stem ratio had a high mean broad sense heritability (82%), whereas HI, grain yield and above ground biomass had lower heritabilities, 68, 55 and 35% respectively. Ear:stem ratio was strongly correlated between generations and sites indicating stability of this character. Ear:stem ratio had a significant positive correlation with grain yield, HI, grains per ear and per m2. The correlation of grain yield with HI was equal or slightly higher than that of grain yield with ear:stem ratio. Ear:stem ratio offers promise as a predictor of HI and yield potential where post-anthesis moisture stress can influence HI. Ear:stem ratio measurement is unlikely to be adopted for selection purposes in routine breeding programs, as it is laborious and time consuming. However, ear:stem ratio could be used to identify superior parental genotypes and early generation selections from special crosses in terms of its ability to partition assimilate.
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  • 58
    ISSN: 1573-5060
    Keywords: hybrids ; crosses ; spelt ; Triticum spelta ; wheat ; Triticum aestivum ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary F1 and F2 hybrids were produced using three winter wheat varieties (Bernina = biscuit wheat, Arina and Forno = bread wheat) and two spelt cultivars (Oberkulmer and Rouquin). Data are based on field trials in 1989 and 1990. All the combinations tested gave a very high relative heterosis on grain yield per ear, varying between 29.9% and 47.6% with an average of 42.1%. Even the higher parent was outyielded by 24.1% to 45.7%. This was the result of a heterosis effect on the two yield parameters grain number per ear and single grain weight. In the F2, heterosis for grain yield per ear was not significant with a range between 3.9% and 12.0%. F1 hybrids showed a heterosis effect on plant height but no hybrid was taller than the spelt parents. These data show that the gene pool of spelt could be potentially usefull for the selection of parents for hybrid production. For practical applications there remain several problems to be solved. Despite the very high heterosis effect the yield potential of spelt needs to be improved. No short spelt varieties exist at present to produce hybrids short enough for commercial application. Technical problems in the seed production arise because of the close tightness of the spelt glumes hampering either the pollination by the wheat parent or the pollen dispersal if used as a pollinator. However, a breeding effort to select for improved parents within the gene pool of spelt appears useful for future F1 hybrids between spelt and wheat.
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  • 59
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    Euphytica 74 (1993), S. 109-115 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Puccinia striiformis ; yellow rust ; adult-plant resistance ; disease resistance breeding strategies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The stripe rust resistant spring wheat (Triticum aestivum L.) varieties ‘Anza’, ‘Glennson 81’, ‘Ollanta’, and ‘Yecora Rojo’ gave 1,2,2, and 2-gene segregations, respectively, in hybrids with susceptible ‘Jupateco 73’ when inoculated in field conditions at Davis, California USA with Puccinia striiformis West. pathotype CDL-6 and rated at post-heading stage. Intercrosses of these varieties, ‘Anza’/‘Yecora Rojo’ was not studied, permitted the following conclusions about the genes expressed in adult plants: ‘Anza’, one recessive gene; ‘Glennson 81’, two dominant genes; ‘Ollanta’, two genes, at least one is dominant; and ‘Yecora Rojo’; one dominant and one recessive gene, one of which is common with ‘Ollanta’. The resistance genes in these varieties, which expressed resistance in the seedling stage, were believed to be effective at the adult stage. Thus, seven resistance genes were identified in the four varieties. The genotypes were designated for the purposes of this study as follows: ‘Anza’, YrA YrH; ‘Glennson 81’, Yr9, YrJ, ‘Ollanta’ YrL YrD; and ‘Yecora Rojo, YrC YrD. It was recommended that these and other Yr genes be used as multiple gene complexes to increase durability of resistance to P. striiformis, an organism known to evolve virulence rapidly in field conditions. The demonstrated durability of ‘Anza’ in California may be a result of its combination of resistance alleles at two loci.
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  • 60
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    Biology and fertility of soils 9 (1990), S. 281-282 
    ISSN: 1432-0789
    Keywords: Surface sterilization ; Contaminants ; Sterile plant selection ; Axenic seedlings ; Triticum aestivum ; Trifolium pratense ; Trifolium repens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sterile seedlings are required for the investigation of interactions between microorganisms and plants. The present study was designed to develop a simple and reliable method for the selection of sterile seedlings by the use of liquid nutrient media, avoiding some of the disadvantages of solid media. The method of germinating surface-sterilized seedlings on solid water agar or nutrient media was compared with our method for sterility control in nutrient broth. Sterile plant selection in liquid media was the most sensitive method for detecting bacterial and fungal contaminants. Sterile plants grow with the same vigour as unsterilized plants and can be used for sterile plant experiments.
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  • 61
    ISSN: 1432-0983
    Keywords: Secale cereale ; Triticum aestivum ; Ribosomal RNA genes ; Mitochondrial DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial 18S and 5S ribosomal RNA (rRNA) genes of rye, plus a total of about 90 kilobase pairs of flanking DNA, have been cloned and maps of restriction enzyme cleavage sites have been constructed. Like their homologs from hexaploid wheat, the rye genes are closely linked and are part of a three-copy family of recombining repeats (the “18S/5S repeat”). The rye repeat probably also contains a mitochondrial tRNAfMet gene, which the wheat repeat is known to carry. However, despite the overall organizational similarity between the wheat and rye 18S/5S repeats in the immediate vicinity of their coding regions, extensive rearrangement of flanking sequences has taken place during evolutionary divergence of the two species. Our data provide additional support for an emerging picture of plant mitochondrial genomes as evolving much more rapidly in structure than in sequence.
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  • 62
    ISSN: 1432-0983
    Keywords: Triticum aestivum ; Mitochondrial DNA ; Chloroplast DNA ; ATPase alpha subunit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An internal part of the chloroplast atpA gene has been identified in the mitochondrial DNA of Triticum aestivum. It is located near the 18S-5S ribosomal genes and partially contained within a repeated sequence. Comparison of the transferred sequence with the original ct sequence reveals several nucleotide changes and shows that neither 5′ nor 3′ ends are present in the mt genome. No transcript of this region could be detected by Northern analysis. This sequence is present in mitochondrial genomes of other tetraploid and diploid species of Triticum, also in the vicinity of the 18S-5S ribosomal genes, suggesting a unique transfer event. The date of this event is discussed.
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  • 63
    ISSN: 1432-1939
    Keywords: Multispecies canopy model ; Canopy photosynthesis model ; Triticum aestivum ; Avena fatua ; Ultraviolet-B radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Competition for light among species in a mixed canopy can be assessed quantitatively by a simulation model which evaluates the importance of different morphological and photosynthetic characteristics of each species. A model was developed that simulates how the foliage of all species attenuate radiation in the canopy and how much radiation is received by foliage of each species. The model can account for different kinds of foliage (leaf blades, stems, etc.) for each species. The photosynthesis and transpiration for sunlit and shaded foliage of each species is also computed for different layers in the canopy. The model is an extension of previously described single-species canopy photosynthesis simulation models. Model predictions of the fraction of foliage sunlit and interception of light by sunlit and shaded foliage for monoculture and mixed canopies of wheat (Triticum aestivum) and wild oat (Avena fatua) in the field compared very well with measured values. The model was used to calculate light interception and canopy photosynthesis for both species of wheat/wild oat mixtures grown under normal solar and enhanced ultraviolet-B (290–320 nm) radiation (UV-B) in a glasshouse experiment with no root competition. In these experiments, measurements showed that the mixtures receiving enhanced UV-B radiation had a greater proportion of the total foliage area composed of wheat compared to mixtures in the control treatments. The difference in species foliage area and its position in the canopy resulted in a calculated increase in the portion of total canopy radiation interception and photosynthesis by wheat. This, in turn, is consistent with greater canopy biomass of wheat reported in canopies irradiated with supplemental UV-B.
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  • 64
    ISSN: 1573-1561
    Keywords: Allelochemicals ; no-tillage ; conventional-tillage ; soils ; wheat ; Triticum aestivum ; mass spectrometry ; Petri-dish bioassay ; fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Putative allelochemicals found in the soil of no-tillage and conventional-tillage wheat plots near Stillwater, Oklahoma, were obtained by a mild alkaline aqueous extraction procedure, bioassayed to determine their biological activity, purified, and analyzed with a capillary gas chromatography-mass spectrometry-data analysis system. The most significant inhibition was found in bioassays of extracts from soil collected immediately after harvest in June, July, and August. No-tillage soils produced significant inhibition during the rest of the year also. Mass spectrometry showed fatty acids as the most abundant compounds. However, when bioassayed authentic samples of the five free fatty acids showed no significant biological activity toward wheat.
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  • 65
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    Journal of chemical ecology 16 (1990), S. 1927-1940 
    ISSN: 1573-1561
    Keywords: Allelopathy ; phytotoxic ; allelochemical ; α-bisabolol ; δ-cadinene ; p-cymen-9-ol ; essential oil ; germination ; phenethyl alcohol ; piperitenone ; vanillin ; Gossypium hirsutum ; Proboscidea louisianica ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The potential allelopathic activity of devil's-claw [Proboscidea louisianica (Mill.) Thellung] essential oil and a few of the compounds it contains on the elongation of cotton (Gossypium hirsutum L.) and wheat (Triticum aestivum L.) radicles was studied using a Petri dish bioassay. Essential oil was collected by steam distillation using an all-glass-Teflon assembly. Ether extracts of the steam distillates from fresh devil's-claw were inhibitory to cotton and wheat radicle elongation. The following six components of devil's-claw essential oil identified by CGC-MS-DS were inhibitory to cotton and/or wheat at a concentration of 1 mM: vanillin, piperitenone, δ-cadinene,p-cymen-9-ol, α-bisabolol, and phenethyl alcohol.
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  • 66
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    Plant molecular biology 15 (1990), S. 947-950 
    ISSN: 1573-5028
    Keywords: clpP gene ; rps12 gene ; ribosomal protein S12 ; intron ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 67
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    Plant cell reports 9 (1990), S. 14-16 
    ISSN: 1432-203X
    Keywords: Triticum aestivum ; Anther culture ; Sugars ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of employing different sugars in wheat anther culture has been investigated using four Spring wheat cultivars. The most responsive cultivar, Orofen, gave a three to four-fold increase in embryo yield when maltose was used in place of sucrose, with 50 embryos being produced for every 100 anthers cultured. Measurement of sugar concentrations in the culture media indicated that sucrose was more rapidly hydrolysed than maltose. However, neither the osmotic potential of the medium nor the concentration of glucose appeared to be critical factors in determining embryo yield.
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  • 68
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    Theoretical and applied genetics 79 (1990), S. 401-410 
    ISSN: 1432-2242
    Keywords: Puccinia graminis tritici ; Puccinia recondita Tritici ; Triticum aestivum ; Rust resistance ; Gene identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genes for resistance toPuccinia graminis tritici andPuccinia recondita tritici identified in four South African wheats were:Sr6,Sr8a,Sr9e, andLr13 in ‘W3762’;Sr5,Sr8a,Sr9b,Sr12,Sr24,Lr13, andLr24 in ‘W3760’;Sr2,Sr24,SrC,Lr13, andLr24 in ‘W3751’; andSr7a,Sr23,Sr36, andLr16 in ‘W3755’. GenesSr2,Sr9e, andSr24 also conferred adult plant resistance to the predominant pathotypes ofP. graminis tritici. GenesSr7a,Sr23, andSrC, when present alone, did not confer acceptable adult plant resistance, even though low seedling reactions were associated with them when tested with the same pathotypes. Genetic recombination betweenLr13 andSr9e was estimated at 12.5%±2.3%.
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  • 69
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    Theoretical and applied genetics 79 (1990), S. 77-80 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Anther culture ; 1B/1R translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The in vitro microspore androgenesis reaction of 25 commercial German spring (including 4 Triticum durum) and 50 winter wheat cultivars was investigated. Tremendous genotypical differences were found in microspore response. The best-responding winter wheat cultivai, “Florida”, is characterized by the presence of a 1B/1R wheat-rye translocation chromosome. The significance of this finding and other genetic systems for future use of haploids in plant breeding is discussed.
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  • 70
    ISSN: 1432-2242
    Keywords: Cereals ; Gramineae ; Somatic embryogenesis ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Immature embryos, inflorescences, and anthers of eight commercial cultivars of Triticum aestivum (wheat) formed embryogenic callus on a variety of media. Immature embryos (1.0–1.5 mm long) were found to be most suitable for embryogenic callus formation while anthers responded poorly; inflorescences gave intermediate values. Immature embryos of various cultivars showed significant differences in callus formation in response to 11 of the 12 media tested. No significant differences were observed when the embryos were cultred under similar conditions on MS medium with twice the concentration of inorganic salts, supplemented with 2,4-D, casein hydrolysate and glutamine. Furthermore, with inflorescences also no significant differences were observed. Explants on callus formation media formed two types of embryogenic calli: an off-white, compact, and nodular callus and a white compact callus. Upon successive subcultures (approximately 5 months), the nodular embryogenic callus became more prominent and was identified as ‘aged callus’. The aged callus upon further subculture, formed an off-white, soft, and friable embryogenic callus. Both the aged and friable calli maintained their embryogenic capacity over many subculture passages (to date up to 19 months). All embryogenic calli (1 month old) from the different callus-forming media, irrespective of expiant source, formed only green shoots on regeneration media that developed to maturity in the greenhouse. There were no significant differences in the response of calli derived from embryos and inflorescences cultured on the different initiation media. Also, the shoot-forming capacity of the cultivars was not significantly different. Anther-derived calli formed the least shoots. Aged and friable calli on regeneration media also formed green shoots but at lower frequencies. Plants from long-term culture have also been grown to maturity in soil.
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  • 71
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    Theoretical and applied genetics 80 (1990), S. 359-365 
    ISSN: 1432-2242
    Keywords: Helminthosporium ; Fusarium ; Phytophthora ; Hordeum vulgare ; Triticum aestivum ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Because plant cells cultured in vitro express genetic variability and since they can be regenerated into functional plants, procedures have been designed to use this system for the production of plants with new important agronomic characteristics, particularly for disease resistance. For barley, wheat, and potato somaclones have been found that were less susceptible to a toxin of Helminthosporium, fusaric acid, Fusarium coeruleum, F. sulphureum, or Phytophthora infestans, when screened in the first in-vitro-derived generation. Here the progeny of such somaclones is evaluated after natural and artificial infection, using greenhouse-grown or field material. The progenies of the same somaclones did not express detectable differences, which indicated that no heterozygous mutations occurred. Most lines and clones differed in their level of susceptibility to the pathogen compared to the level of the starting material, but these data were in no instance significant. It is discussed here whether this lack of significance is due to a lack of genetic differences or whether the test procedures are in adequate for detecting and securing the slight, probably quantitative, alterations.
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  • 72
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Glutenin ; Gliadin ; Aneuploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The endosperm storage proteins, glutenin and gliadin, are major determinants of bread-making quality in hexaploid wheat. Genes encoding them are located on chromosomes of homoeologous groups 1 and 6. Aneuploid lines of these groups in spring wheat cultivar ‘Chinese Spring’ have been used to investigate the effect of varying the dosage of chromosomes and chromosome arms upon bread-making quality, where quality has been assessed using the SDS-sedimentation test. Differences between the group 1 chromosomes for quality were greater than those between the group 6 chromosomes. The chromosomes were ranked within homoeologous groups for their effect on quality as follows (〉=better quality): 1D〉1B〉1A and 6A〉6B=6D. The relationship of chromosome dosage with quality was principally linear for four of the chromosomes, but not for 6B and 6D. Increases in the dosage of 1B, 6A and, especially, 1D, were associated with significant improvements in quality, whereas increases in the dosage of 1A were associated with reductions in quality. The effects of 1A and 1D were such that the best genotype for quality was nullisomic 1A-tetrasomic 1D. For group 1, effects of the long arm appeared in general to be more important than effects of the short arm. For group 6, effects were found associated with the long arms as well as with the short arms, a surprising result in view of the absence of genes encoding storage proteins on the long arms. Significant interactions were found between chromosomes and genetic backgrounds, and between individual chromosomes. Analysis of trials grown over two years demonstrated that, although additive environmental differences over years and genotype x years interaction were present, they were relatively small in magnitude compared with purely genetic differences.
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  • 73
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    Protoplasma 153 (1990), S. 193-203 
    ISSN: 1615-6102
    Keywords: Endosperm ; Intercellular protoplasmic movement ; Plasmodesma ; Nuclear migration ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Earlier work in our laboratory indicated that protoplasmic constituents can migrate from one cell to another in certain tissues of higher plants. Further investigations have been conducted using garlic bulbs and wheat nucellus for microscopic observation of intercellular protoplasmic movement in vivo. These gave preliminary indications of the dynamic characteristics of migrating nuclei and cytoplasm. The present paper gives recent results providing new evidence for intercellular protoplasmic movement that is neither hindered by the presence of cell wall nor the narrowness of channels of intercellular connection. By careful manipulation, intact endosperm sacs could be taken from developing caryopses (6–8 days after fertilization) without apparent injury to constituent cells. Shortly after the living specimen is mounted on the microscopic stage, asynchronous intercellular protoplasmic movement can be observed here and there. It can be seen that protoplasm extrudes in rapid but intermittent movements from one cell to the next by vigorous contraction. Although various cell constituents may move together, they can also be quite independent of each other. The moving units, though undergoing violent deformation, resume their normal shape and structure following intercellular migration. Evidently this kind of movement is a naturally occurring and active phenomenon closely related to the physiological state of the tissue. Electron microscopic studies reveal that a limited number of plasmodesmatal channels undergo modification and enlarge to 100–400 nm, through which the protoplasmic constituents pass.
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  • 74
    ISSN: 1573-8469
    Keywords: yellow (stripe) rust races ; Triticum aestivum ; Yr genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Onder geconditioneerde klimaatsomstandigheden zijn 42 Ethiopische en CIMMYT rassen en lijnen van broodtarwe (Triticum aestivum) in het kiemplantstadium geïnoculeerd met 19 isolaten van gele roest die onderling verschilden in hun pathogeniteit voor 20 differentiërende tarwerassen waarvan de resistantie-achtergrond bekend is. De genom-gen relatie is toegepast om resistentiegenen te identificeren. Vier rassen en lijnen bleken resistent te zijn tegen alle isolaten. Verondersteld wordt dat hun resistentie berust op genen die niet eerder herkend waren of op een combinatie van bekende genen die niet compatibel was met de gebruikte isolaten. In het overige tarwemateriaal kon de aanwezigheid worden aangegeven van de resistentiegenenYr2, Yr3, Yr4, Yr6, Yr7, Yr9 enYrA. Het van rogge afkomstige en door het CIMMYT veel gebruikte resistentiegenYr9 was in 28 rassen en lijnen (67%) aanwezig. In het onderzochte tarwemateriaal isYr4 het enige voor Oost en Centraal Afrika effectieve resistentiegen omdat de daar voorkomende gele roest pathogeniteit bezit voor de overige genen. Het herkennen van pathogeniteit van gele roest voor bepaalde resistentiegenen is verbeterd door het toevoegen van tarwerassen met monogene resistentie aan het internatinale gebruikte tarwesortiment voor de determinatie van gele-roestfysio's.
    Notes: Abstract In a controlled environment, the reaction was observed of 42 bread wheat varieties and lines inoculated with 19 isolates of yellow rust differing in their virulence to 20 differential varieties. Five varieties and lines showed resistance to all isolates. The remaining ones appeared to have the genesYr2, Yr3, Yr4, Yr6, Yr7, Yr9 andYrA, either singly or in combination.Yr9 derived from rye was present in 67% of the varieties and lines.Yr4 is the only effective gene in that material as, in Eastern and Central Africa, yellow rust has virulence to the otherYr genes. Recognition of virulence toYr genes is enhanced by the use of a supplemental set of differential varieties supposedly carrying a single gene.
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  • 75
    ISSN: 1573-8469
    Keywords: Puccinia recondita ; Triticum aestivum ; Photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting De relatie stussen de epidemiologie van bruine roest, bladveroudering en oprengstderving werden bestudeerd bij zeven wintertarwegenotypen die verschilden in partiële resistentie tegen bruine roest. Bruine roest versnelde de veroudering van blad en aar. Fotosynthesemetingen wezen echter uit dat de fotosynthesecapaciteit van het resterende groene oppervlak niet werd beïnvloed. Verschillen tussen de genotypen in opbrengstderving werden voornamelijk veroorzaakt door verschillen in bladveroudering en daarmee in cumulatieve lichtinterceptie (r=0.83) en gewasfotosynthese. De reductie in cumulatieve lichtinterceptie was nauw gerelateerd aan de cumulatieve ziektedruk (in puistdagen) (r=0.85), welke ook nauw was geassocieerd met de opbrengstderving (r=0.88). De opbrengstreductie werd echter even goed voorspeld door de tijdsduur tot 50% reductie van het groene bladoppervlak en de puistdichtheid halverwege de epidemie als door cumulatieve lichtinterceptie en cumulatieve ziektedruk, waardoor beide eerste criteria gebruikt kunnen worden in de selectie voor partiële resistentie tegen bruine roest.
    Notes: Abstract Relations between leaf rust progress, foliage senescence and yield reduction were studied for seven winter wheat genotypes, differing in their partial resistance to leaf rust. Leaf rust accelerated leaf and ear senescence. Photosynthesis measurements showed, however, that the photosynthetic capacity of the remaining green surface was not affected. Differences between genotypes in yield reduction were largely explained by differences in leaf senescence and, therefore, in cumulative light interception (r=0.83) and crop photosynthesis. Reduction in cumulative light interception was closely related to the area under the disease-progress-curve (r=0.85), which was also closely associated with yield reduction (r=0.88). However, the time taken to reach a 50% reduction of green leaf area and the pustule density on 1 July (i.e. halfway through the epidemic) were just as good predictors of yield reduction as cumulative light interception and area under disease-progress-curve, suggesting that the former are useful criteria in the selection for partial resistance to leaf rust.
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    European journal of plant pathology 96 (1990), S. 29-34 
    ISSN: 1573-8469
    Keywords: Triticum aestivum ; simulation ; SUCROS87 ; NWHEAT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Om systemen voor geleide bestrijding van ziekten te verbeteren, werd de vraag gesteld of schaderelaties, vastgesteld in jaren met specifieke weersomstandigheden, ook gebruikt kunnen worden in andere jaren. Twee bestaande modellen van tarwe en een combinatie van beide, werden gebruikt om het effect van weer op opbrengst te berekenen, eerst bij afwezigheid van meeldauw. De berekende opbrengsten in verschillende jaren toonden geen samenhang met de behaalde opbrengsten, gecorrigeerd voor een jaarlijkse toename, in Nederland. Verschillen tussen de modellen konden worden toegeschreven aan de wijze waarop de groei en ontwikkeling van tarwe vroeg in het seizoen wordt berekend.
    Notes: Abstract A vital question to upgrade disease management systems is whether damage functions, established in years with specific weather conditions, can be applied also in other years. A simulation approach was used to explore effects of weather on yield and damage. Two existing models of wheat and a third, a combination of both, were used to compute yield, first in absence of mildew. In a second paper, effects of mildew will be reported. Yields simulated for different years were not significantly correlated with yields harvested, adjusted for their increase over years, in the Netherlands. Differences in performance between the models could be attributed to the method of simulating development and growth early in the cropping season.
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  • 77
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; genotypes ; interaction ; manganese ; oxidation ; take-all ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Take-all is a world-wide root-rotting disease of cereals. The causal organism of take-all of wheat is the soil-borne fungus Gaeumannomyces graminis var tritici (Ggt). No resistance to take-all, worthy of inclusion in a plant breeding programme, has been discovered in wheat but the severity of take-all is increased in host plants whose tissues are deficient for manganese (Mn). Take-all of wheat will be decreased by all techniques which lift Mn concentrations in shoots and roots of Mn-deficient hosts to adequate levels. Wheat seedlings were grown in a Mn-deficient calcareous sand in small pots and inoculated with four field isolates of Ggt. Infection by three virulent isolates was increased under conditions which were Mn deficient for the wheat host but infection by a weakly virulent isolate, already low, was further decreased. Only the three virulent isolates caused visible oxidation of Mn in vitro. The sensitivity of Ggt isolates to manganous ions in vitro did not explain the extent of infection they caused on wheat hosts. In a similar experiment four Australian wheat genotypes were grown in the same Mn-deficient calcareous sand and inoculated with one virulent isolate of Ggt. Two genotypes were inefficient at taking up manganese and were very susceptible to take-all, one was very efficient at taking up manganese and was resistant to take-all, and the fourth genotype was intermediate for both characters. All genotypes were equally resistant under Mn-adequate conditions.
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    Euphytica 45 (1990), S. 71-80 
    ISSN: 1573-5060
    Keywords: wheat ; Triticum aestivum ; wheat leaf rust ; Puccinia recondita f.sp. tritici ; partial resistance ; histology ; growth curve
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The average size of wheat leaf rust colonies, measured using epifluorescence microscopy was significantly larger in the highly susceptible genotype Morocco than in the susceptible genotype Kaspar and the partially resistant genotypes Westphal 12A, Akabozu and BH 1146. This was already so three days after inoculation. Colony growth in partially resistant genotypes was continuously retarded compared to colonies in the highly susceptible genotype Morocco. No evidence was found for an initial inhibition of the growth of colonies in partially resistant genotypes. In partially resistant genotypes formation of uredial beds and sporulating areas started at a smaller colony size than in susceptible genotypes. Wheat leaf rust colonies in primary leaves of all genotypes studied were much larger than colonies in flag leaves measured at the same number of days after inoculation. Growth and sporulation of not intertwined colonies was not influenced by either a high or a low number of neighbouring colonies.
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    Euphytica 45 (1990), S. 81-86 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; wheat leaf rust ; partial resistance ; histology ; abortion ; adult plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Arrest of the growth of wheat leaf rust infection structures was studied with fluorescence microscopy in seedling leaves and flag leaves of the susceptible spring wheat genotypes Morocco and Kaspar and the partially resistant genotypes Westphal 12A and Akabozu. The percentages non-penetrants and substomatal vesicle abortion were low in all genotypes. In the partially resistant genotypes the percentage abortion of infection structures was higher than in the susceptible genotype Morocco. Aborted infection structures had formed one or two haustorial mother cells. In adult plants differences in the percentage aborted infection structures between susceptible and partially resistant genotypes were more pronounced than in seedlings. The so-called late abortion was not observed.
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    Euphytica 45 (1990), S. 59-69 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Pyrenophora tritici-repentis ; resistance ; tan spot ; yellow spot ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary No complete resistance to Pyrenophora tritici-repentis has been located in more than 1400 bread wheats examined. Incomplete resistance, however, occurs at different levels in many spring and winter types and data are presented for the strongest sources of resistance detected. In particular, there is a high frequency of Brazilian spring wheats with appreciable levels of resistance to this pathogen. Recent international nurseries from CIMMYT, Mexico, also contain numerous potentially valuable sources of resistance and these wheats may be shorter and higher yielding in Australia than the Brazilian wheats. The resistances in many Brazilian cultivars may be largely common because the cultivars are often strongly interrelated. Some of the Brazilian wheats resistant to P. tritici-repentis are also resistant to one or more of the septoria diseases and/or possess tolerance to aluminium toxicity.
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  • 81
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    Euphytica 45 (1990), S. 87-92 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; near-isogenic lines ; NILs ; Puccinia recondita f.sp. tritici ; leaf rust ; Puccinia striiformis ; yellow rust ; backcross ; variation ; background resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using the cultivar Arina as the recurrent parent, six backcrosses were made with two donor lines carrying the leaf rust resistance genes Lr1 and Lr9, respectively. Selection for leaf rust resistance occurred at the seedling stage in the greenhouse; the first plants transferred to the field were BC6F4s. Frequency distribution of the 332 Lr1/7 × Arina and the 335 Lr9/7 × Arina lines showed continuous variation for yellow rust resistance and heading date in these leaf rust near-isogenic lines (NILs). Similar results were also obtained for plant height, for resistance to powdery mildew and glume blotch, as well as for baking quality characters in another set of more advanced NILs. The available information on the behaviour of one of the parents of cultivar Arina led to the conclusion that the expressed yellow rust resistance is quantitative and might possibly be durable.
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  • 82
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    Euphytica 45 (1990), S. 169-177 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; coefficient of parentage ; pedigree ; gene pool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Pedigrees of 142 Yugoslavian winter wheat cultivars were traced to 110 ancestral genotypes, of which 41 contributed significantly. In each of the four major Yugoslavian wheat breeding programs, the most important ancestor, as evaluated by mean coefficient of parentage, was ‘Akagomughi’, source of the genes Rht8 and Ppd1. The other 13–19 ancestors accounting for the majority of the remaining germplasm, varied considerably among institutions. The relative contributions of ancestors changed little between the periods 1967–76 and 1982–86, with the exception of ‘Neuzucht’ (source of a 1B/1R translocation), which became much more important in the latter period.
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  • 83
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    Euphytica 46 (1990), S. 149-155 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; selection ; preharvest sprouting ; germination ; kernel color ; dormancy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The utility of spike- and seed-based mass selection techniques for improving preharvest sprouting resistance in heterogeneous wheat (Triticum spp.) populations was evaluated. Sorting seed by size improved selection efficiency in some cases, putatively by physiological synchronization. Progeny testing, as well as changes in frequency of red-kernelled types, indicate effectiveness of both spike- and seed-based mass selection for reduced preharvest sprouting. Differential effectiveness of mass selection, in populations segregating for dormancy from different sources, is consistent with previous work on mechanisms of dormancy from these sources. These results are of value to improvement of preharvest sprouting resistance in large, heterogeneous wheat populations.
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  • 84
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    Euphytica 47 (1990), S. 165-169 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; isozyme loci ; esterase ; homoeology relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The EST-6 leaf esterase phenotypes from euploid, nullisomic-tetrasomic and rye chromosome addition and substitution lines of common wheat were determined using polyacrylamide gel electrophoresis. Evidence is presented to demonstrate that Est-6 is a new set of genes, that are expressed in the leaf. The Est-6 gene set were clearly distinguished from the Est-5 genes which are expressed in the grain. The three homoeoallelic loci, Est-A6, Est-B6 and Est-D6, were located on chromosomes 3A, 3B and 3D. An Est-R6 gene was located on chromosome 6R is involved in rye. Some considerations concerning homoeology between homoeologous group 3 of wheat and the rye chromosome 6R are made.
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  • 85
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Puccinia striiformis ; yellow rust ; stripe rust ; Puccinia recondita f. sp. tritici ; brown rust ; leaf rust ; inheritance ; rust resistance ; monosomic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A set of 21 monosomics of Novosadska Rana-1 was used to locate the rust resistance genes of Lüqiyu, a stripe rust resistant line developed by BAU and Yantar, a leaf rust resistant wheat introduced from Bulgaria. The resistance of the former to p. striiformis race C25 was conditioned by a dominant gene located on chromosome 2B, whereas that of the latter to P. recondita race CL3 was controlled by two complementary dominant genes located on chromosomes 5A and 1D, respectively. The relationship of the stripe rust resistance gene in Lüqiyu to Yr5, Yr7 or Yr Suwon' all located on chromosome 2B is unknown. The two complementary leaf rust resistance factors in Yantar appear to be new.
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  • 86
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    Euphytica 48 (1990), S. 1-8 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; protein accumulation ; plant protein ; protein estimation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Better understanding of the physiological and genetic basis of wheat grain protein will contribute to breeding efforts for this characteristic. This study provides information about plant protein distribution in high and low grain protein winter wheats (Triticum aestivum L.) at different growth stages and its relation to grain protein. Field experiments involved two winter wheats with high grain protein, ‘Redwin’ and ‘Lancota’, and two with low grain protein, ‘Centurk’ and ‘Brule’ in two years. Protein content in the head, the upper three leaves, the first and second leaf, and the peduncle were estimated with Near Infrared Reflectance Spectrophotometer (NIR) at five growth stages. High protein cultivars had higher leaf protein at ripe and higher protein content in the heads at most growth stages than low grain protein cultivars. High protein cultivars had lower protein content in the peduncle than low protein cultivars at ripe. Correlation coefficients between plant-part protein and grain protein ranged from 0.48 to 0.87 for the heads, from −0.45 to −0.79 for the peduncle, and from 0.55 to 0.84 for the leaves. A combination of head, peduncle, and first leaf protein at heading was significantly related to grain protein (R2=0.71). Indirect selection for head, peduncle, and first leaf (flag leaf) protein at heading should result in increased grain protein. Recurrent selection for increased grain protein, with parent selectionbefore anthesis and hybridization should be successful.
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  • 87
    ISSN: 1573-5060
    Keywords: Sorghum bicolor ; sorghum ; Triticum aestivum ; wheat ; Oryza sativa ; rice ; Fraction-1-Protein inheritance ; Isoelectric focusing ; intergeneric hybrids ; Large and small sub-units ; rice × sorghum ; rice × wheat hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The polypeptide composition of Fraction-1-Protein (F1P) from rice × sorghum, rice × wheat hybrids and their respective parents have been analyzed by a microelectrofocusing method. The large sub-unit (LSU) is composed of three polypeptides and the small sub-unit (SSU) of two polypeptides in rice and sorghum parents and rice × sorghum hybrids. Similarly, LSU is composed of three polypeptides in the rice and wheat parents and rice × wheat hybrids. Two polypeptides occur in the SSU of rice parent and rice × wheat hybrids where as only one polypeptide in the wheat parent. These polypeptides also differ in their isoelectric points. Based on the previous reports of F1P inheritance in hybrids in other crops, F1P analysis of rice × sorghum and rice × wheat hybrids does not seem to be an important marker to identify such intergeneric hybrids. Since this is first such report of F1P inheritance in hybrids between distantly related plants, its implication in different modes of inheritance are discussed.
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  • 88
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; detached ear culture ; immature seed vernalization ; narrow-sense earliness ; photoperiodic response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of the addition of sulfurous acid into culture solution and of cold treatment of the solution were examined to simplify the culture of detached wheat ears. In the simplified method, detached ears could be cultured at room temperature on the liquid medium containing 100 g/l sucrose and 0.075% sulfurous acid without any sterilization. The immature seeds in detached ears cultured by this method were treated with low temperature or with chemicals known to have vernalizing effect. The chemical treatment did not affect the chilling requirement of immature embryos, although photoperiodic response and narrow-sense earliness were reduced by kinetin and trypsin. The low temperature treatment drastically affected the chilling requirement, and fully vernalized mature seeds having normal germinability were obtained by treating the detached ears in culture with low temperature from 10 days after anthesis.
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  • 89
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    Euphytica 49 (1990), S. 203-207 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; Sorghum bicolor ; Triticum aestivum ; C3–C4 characters ; growth analysis ; rice × sorghum ; rice × wheat hybrids ; root studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Some photosynthetic characters as-leaf anatomy, leaf photosynthetic rate and CO2 compensation pointdistinguishing C3 and C4 plants and physiological characters as leaf area ratio, nitrogen content, leaf stem ratio and total shoot-to-root and deep root-to-shoot ratios have been studied in rice × sorghum and rice × wheat hybrids. Rice × sorghum 1. has lower values of photosynthetic rate, leaf nitrogen, total root and deep root-to-shoot ratio and CO2 compensation point as of rice parent where as, rice × sorghym 2. is superior in all these characters. Both hybrids lack kranz anatomy. Though both rice × sorghum hybrids show characters of C3 rice plant but rice × sorghum 2. has improved drought tolerance and leaf characters in relation to yield. Rice × wheat hybrid have higher assimilatory area and higher total root-to-shoot ratio. Grains of rice × wheat hybrids are identical to rice grain. However, as grains of rice × wheat hybrid does not contain seed coat, it could be exploited as novel rice germplasm after improvement.
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  • 90
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    Euphytica 49 (1990), S. 155-159 
    ISSN: 1573-5060
    Keywords: Secale cereale ; rye ; rye-wheat-additions ; Triticum aestivum ; wheat ; anthocyanins ; purple leaf base ; purple/red auricles ; gene location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary ‘Purple leaf base’ is expressed only if there is anthocyanin pigmentation in coleoptiles either in rye or in rye-wheat-additions. Genes controlling ‘purple leaf base’ were found to be located on chromosomes 5R (An5), 4B (Ra2) and 6B (Ra3) using the trisomic set of rye cv. Esto and autoplasmic rye-wheat-additions, respectively.
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  • 91
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; drought resistance ; grain yield ; relative water content ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Water is often the most limiting factor to winter wheat (Triticum aestivum L.) production in the southern Great Plains of the U.S.A., yet the lack of reliable screening criteria has precluded direct selection for drought resistance in breeding programs. Previous work showed that leaf relative water content (RWC) was highly heritable when measured under field-drought conditions, but its adoption as a screening tool for yield improvement requires further investigation of the genetic relationship between grain yield and RWC. Plants representing high and low yield potential under drought stress, and a random group of plants, were selected from an F2 population having the pedigree, TAM W-101/Sturdy. Two sets of entries, each comprised of the two parents and 24 F2-derived lines, were evaluated under a rainshelter in the F3 (1986) and F4 (1987) generations to determine differences in leaf RWC during reproductive development. One set of entries did not receive any water after the jointing stage, and the other set was grown under well-watered conditions. A positive relationship was observed between grain yield and RWC measured during anthesis and mid-grain fill, as the high-yield selections maintained a significantly higher RWC than the low-yield selections. Grain yield and RWC were also positively associated among random selections segregating for both traits. Subsequent adjustment of genotype means for differences in reproductive development at time of sampling underscored the need to consider differences in maturity when RWC is the selection criterion.
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  • 92
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    Euphytica 50 (1990), S. 11-18 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Fusarium culmorum ; head blight ; scab ; resistance ; gene action ; number of genes ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crosses were made among ten winter wheat genotypes representing different levels of resistance to Fusarium head blight to obtain F1 and F2 generations. Parents, F1 and F2 were inoculated with one strain of Fusarium culmorum. Data on incidence of head blight 21 days after first inoculation were analyzed. Broad-sense heritabilities averaged 0.39 and ranged from 0.05 to 0.89 in the individual F2 families. The joint-scaling test indicated that the inheritance of Fusarium head blight resistance was adequately described by the additive-dominance model, with additive gene action being the most important factor of resistance. With respect to the non-additive effects, dominance of resistance predominated over recessiveness. The number of segregating genes governing resistance in the studied populations was estimated to vary between one and six. It was demonstrated that resistance genes differed between parents and affected resistance differently.
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  • 93
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat breeding ; genetic improvement with time ; grain nitrogen concentration ; nitrogen economy ; nitrogen utilization efficiency ; Argentina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Changes in nitrogen (N) economy and N to dry matter (DM) relationships were studied for six cultivars of bread wheat (Triticum aestivum L.) released in Argentina at different times between 1912 and 1980. Experiments were performed on two successive years. N partitioning to reproductive organs was changed both at anthesis and at maturity. Grain N yield (GNY) was associated to both total N accumulated and N partitioning. Most of the changes produced by genetic improvement on N economy at maturity could be explained by parallel changes at anthesis. Neither biological N yield (BNY) at anthesis nor BNY at maturity showed any trend with the year of release of the cultivars. Grain N concentration (GNC) showed a negative trend with the year of release, and was inversely correlated to both grain yield (GY) and harvest index (HI). However, GNC was positively and significantly associated with NHI to HI ratio, indicating that the main reason for its behaviour along this century was the dilution of N on non N compounds. The N utilization efficiencies (NUE) for both GY and grain number were positively affected by breeding. Moreover, modern Argentinian cultivars are as efficient as the best cultivars showed by other authors. It is suggested that to increase GNC together with GY, breeders should improve N accumulation at anthesis maintaining high remobilization of vegetative N.
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  • 94
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    Euphytica 50 (1990), S. 1-9 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Fusarium culmorum ; Fusarium head blight ; resistance ; scab ; diallel cross ; combining ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten homozygous winter wheat genotypes representing different levels of resistance to Fusarium head blight were crossed in all possible combinations excluding reciprocals. Parents, F1 and F2 were inoculated with one pathogenic strain of Fusarium culmorum. Data for head blight, observed 21 days after first inoculation (OBS-2), and for the area under the disease progress curve, based on observations 14, 21 and 28 days after first inoculation (AUDPC), were analyzed. The contrast between parents and F1 crosses indicated dommance effects of the resistance genes. Diallel analysis according to Griffing's Method 4, Model 1 showed significant general combining ability (GCA) effects for both F1 and F2; specific combining ability effects were not significant. With the exception of one genotype for which general performance for Fusarium resistance was not in agreement with its GCA, the resistance to F. culmorum was uniformly transmitted to all offspring, and the parents can be described in terms of GCA. It is suggested that in the progenies with one of the awned lines as parent, one resistance gene was linked with the gene coding for presence of awns, located on chromosome 4B. A single observation date, taken at the right time, was as effective in assessing resistance as the AUDPC.
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  • 95
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    Euphytica 50 (1990), S. 221-239 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; gibberellic acid response ; dwarfing genes ; culm length
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The GA response, Rht genes and culm length of 133 Norin varieties, 6 breeding lines and 16 landraces of Japanese wheat were investigated. Out of 133 Norin varieties tested, 103 were GA-insensitive and 30 GA-responsive. The 6 breeding lines were all GA-insensitive. Out of 16 landraces tested, 10 were GA-insensitive and 6 GA-responsive. Among the 10 GA-insensitive landraces, only Daruma had a Rht1 genotype. The other 9 had a Rht2 genotype. None of the landraces tested carried both Rht1 and Rht2 or Rht3. Out of the 103 GA-insensitive Norin varieties, 22 carried only Rht1, another 79 carried only Rht2, and only Norin 10 and Kokeshikomugi carried both Rht1 and Rht2. No tested variety carried Rht3. Some Norin varieties carrying Rht2 showed tall culms comparable to that of the rht tester line Chinese Spring. These results suggest that these varieties had a nullifier or modifier gene(s) or height promoting genes in the background controlling the height-reducing effect of Rht2. Conversely, six GA-responsive Norin varieties were as short as Akakomugi which carries the GA-responsive Rht genes, Rht8 and Rht9. The also seemed to carry a GA-responsive Rht gene or genes, and moreover all but one may carry gene(s) other than the Akakomugi genes. The origin of Rht1 and Rht2 of Norin 10 was speculated on the GA-response and Rht genotypes of its related varieties and landraces.
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  • 96
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    Euphytica 50 (1990), S. 57-62 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; single plant selection for yield ; inter-genotypic competition ; interplot competition ; cultivar degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study was undertaken to explore the relation between auto-, allo- and nil-competition using a long established pure line cultivar of bread wheat, where genes controlling yielding and competing ability co-exist and co-interact. The investigation lasted three growing periods, the first two with trials grown at nilcompetition (100 cm), and the third with trials grown at the three conditions of competition. In the first growing period, divergent single plant honeycomb selection for high and low yield was applied in the cultivar Siete Cerros, to continue in the second growing period with divergent line honeycomb selection. In the third growing period, top and bottom lines selected under nil-competition were compared with the mother variety Siete Cerros under auto-, allo- and nil-competition. Correlation coefficient estimates between auto-, allo- and nil-competition demonstrated that auto-competition and nil-competition are correlated positively interse and negatively with allo-competition. In general, the results suggest that yielding and competing ability are correlated negatively. The implications of the findings are discussed in relation to the possibility of (1) selecting for heritable high yield on a single plant basis, (2) avoiding biased results due to interplot competition, (3) substituting cultivar degeneration for cultivar improvement.
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  • 97
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    Euphytica 51 (1990), S. 77-86 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; dough stickiness ; rye-derived wheat cultivars
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rye-derived wheat cultivars are being used in many breeding programmes throughout the world in order to achieve improvements in yield and disease resistance. However, the serious quality defect of intense dough stickiness associated with many of these wheat cultivars is limiting the usefulness of their flour in large mechanised bread bakeries. A dough preparation procedure was developed which enabled the dough surface properties of a range of rye-derived wheat cultivars to be assessed on doughs mixed quantitatively to their optimum mixing time. Intense dough stickiness was found in samples of 1AL/1RS and 1DL/1RS translocation lines tested and in all of the 1BL/1RS wheat cultivars examined except the West German cultivar, Disponent. Most of the 1BL/1RS wheat cultivars were derived from the Russian cultivars, Kavkaz, Aurora and Skorospelka 35 and included the CIMMYT-bred cultivars such as the Veery lines (Glennson, Ures, Genaro and Seri) and the Nebraskan cultivar, Siouxland. Based on the results of studying selected 1BL/1RS wheat cultivars in detail, this intense dough stickiness appeared to be independent of growing season, trial location, protein content, mixing tolerance, milling process and extraction rate. In addition pilot bakery trials confirmed that our laboratory testing procedures can be used to detect this intense dough stickiness.
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  • 98
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    Euphytica 45 (1990), S. 113-118 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; spring varieties ; Vrn-genotypes ; dominant Vrn-genes ; geographic distribution ; landraces and improved varieties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A study of the global Vrn-genes distribution supports the hypothesis that these loci have different breeding values. Evident zonal differences were discovered in percentage of dominant Vrn-genes and genotypes between the sets of varieties from various locations. For the same zone analogous differences were not so manifested between the sets of local and improved varieties. It showed the similar environmental fitness of those sets as a result of natural or artificial selection. Obvious changes in distribution of Vrn-genes were discovered in the history of breeding. Within this century the dominant Vrn3 was introgressed into many modern varieties of countries close to the equator. The main direction of such introgression ran from the Japanese landrace Akakomugi to Italian varieties. Later it came from Italy through Mentana to Mexican semidwarf varieties, and the last ones (especially Sonora 64 and Lerma Rojo 64) promoted wide distribution of that gene all over the world.
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  • 99
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; indirect selection ; single-plant selection ; honey-comb design ; harvest index ; protein content ; correlation ; regression ; path coefficients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The relationships between yield, its components and other associated characteristics, both within and across generations, were studied in the F2, F3 and F4 of two Hard Red Spring wheat (Triticum aestivum) crosses using simple correlation, path coefficient and step-wise multiple regression analyses. In the F2 and F3 the plants were grown 50 cm apart from each other while in the F4 they were grown under the usual farm practices. Selection was practiced for high and low yield in the F2 and F3 mainly on the basis of individual plant yield. Statistically significant, but not always practically useful, correlations were found between yield and its components and other associated characters. The relationship between yield and protein content was negative and significant within all generations but not so between F2 (and F3) and F4. The intergeneration correlation coefficients between F4 grain yields and grain yields measured in the F2 and F3 were all positive and highly significant. These coefficients, which are also heritability estimates in standard units, were small in magnitude. Stepwise multiple regression analysis identified plant yield as the most significant factor in determining F4 line yield, followed by its components in the order of 1000-kernel weight, grain yield per plant and number of tillers per plant. Path coefficient analysis identified tiller number per plant and grain yield per spike as having strong positive direct effects on single plant yield. Harvest index of individual F2 plants can be used as an indirect selection criterion for yield.
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  • 100
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Hordeum chilense ; Triticum turgidum ; Triticum durum ; durum ; C-banding ; meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The meiotic behaviour of a hybrid between Triticum aestivum and the amphiploid Hordeum chilense x T. turgidum conv. durum, was studied using a C-banding staining method. This hybrid has the genome formula of AA BB D Hch with 2n=6x=42 chromosomes. The durum wheat chromosomes (genomes A and B) were easily recognized, whereas the D and Hch chromosomes were recognized as a whole. Meiotic pairing was homologous, as expected (14 bivalents from A and B genomes +14 univalents from D and Hch genomes). However, some pollen mother cells at metaphase-I presented pseudobivalents that could have been caused by either homoeologous or autosyndetic pairing amongst D and Hch chromosomes.
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