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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cytological evidence for gradual telomere synthesis at the broken chromosome ends in wheat (1993)
    Springer
    Journal of plant research 106 (1993), S. 239-244 
    Details
    ISSN: 1618-0860
    Keywords: Chromosome breakage ; Gametocidal gene ; Healing ; Telomere repetitive sequence ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Telomere formation of the normal and broken chromosomes of common wheat,Triticum aestivum, was investigated byin situ hybridization using the biotin-labeled probe of telomere repetitive sequences (pAtT4) ofArabidopsis thaliana with subsequent amplification by an antibody. After double and triple amplification, prominent signals appeared at all the telomeric regions of the normal chromosomes. Prominent signals also emerged at the broken ends of the telocentric and deletion chromosomes that had passed through more than one generation since the appearance. However, broken ends that had passed through only the stages of gametogenesis, fertilization, embryogenesis and root development did not show complete signals such as found in normal telomeres. These findings indicate that a certain time or stage is required for synthesis of the telomeric repetitive sequences with a complete length. Nevertheless, because the broken ends without complete telomere sequences were also healed, restoration of the normal complement of telomere sequences is not necessary for healing of broken ends.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02344591
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  • 2
    Electronic Resource
    Electronic Resource
    Dynamics of Tandem Repetitive Afa-Family Sequences in Triticeae, Wheat-Related Species (1998)
    Springer
    Journal of molecular evolution 47 (1998), S. 183-189 
    Details
    ISSN: 1432-1432
    Keywords: Key words: Triticeae — Wheat — Afa family — Tandem repeat amplification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The Afa-family sequences in wheat-related species, Triticeae, are tandem repetitive sequences of 340 bp. All the analyzed Triticeae species carried the sequences in their genomes, though the copy numbers varied about 100-fold among the species. The nucleotide fragments amplified by PCR were cloned and sequenced, and their behavior in the evolution of Triticeae was analyzed by the neighbor-joining (NJ) method. The sequences in genomes with many copies of this family clustered at independent branches of the phylogenic tree, whereas the sequences in genomes with a few copies did not. This may suggest that Afa-family sequences had amplified several times in the evolution of Triticeae, each using a limited number of different master copies. In addition, the sequences of the A and B genomes of hexaploid common wheat indicated that the Afa-family sequences had not evolved in a concerted manner between the genomes. Furthermore, the sequences of each chromosome of the D genome of this species indicated that the sequences had amplified on all over the D-genome chromosomes in a short period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006375
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  • 3
    Electronic Resource
    Electronic Resource
    Exclusive Localization of Tandem Repetitive Sequences in Subtelomeric Heterochromatin Regions of Leymus Racemosus (Poaceae, Triticeae) (1999)
    Springer
    Chromosome research 7 (1999), S. 519-529 
    Details
    ISSN: 1573-6849
    Keywords: Afa-family sequences ; Leymus ; subtelomeric heterochromatin ; Triticeae ; 350-bp family sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two kinds of tandem repetitive sequences were isolated from Leymus racemosus (Lam.) Tzvelev. One of them was classified in the 350-bp family originally isolated from Secale. The other was a novel repetitive sequence family, named ‘TaiI family’, which consisted of a repeat unit of 570 bp. Fluorescence in-situ hybridization of the chromosomes of L. racemosus indicated that both families were located in subtelomeric heterochromatin and that the 350-bp family and TaiI family occupied different heterochromatin regions. In addition, even homologous chromosomes did not show the same patterns of TaiI and 350-bp families. The combination of these two families of repetitive sequences, together with Afa-family sequences and rDNAs, helps to identify the ten homologous chromosome pairs of L. racemosus. From these data, we proposed a karyotype of L. racemosus and compared it with other karyotypes already reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009285311247
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  • 4
    Electronic Resource
    Electronic Resource
    A novel repetitive sequence, termed the JNK repeat family, located on an extra heterochromatic region of chromosome 2R of Japanese rye (1999)
    Springer
    Chromosome research 7 (1999), S. 95-102 
    Details
    ISSN: 1573-6849
    Keywords: heterochromatin ; Secale cereale L. ; tandem repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Among cultivated rye, Secale cereale L., collected in Japan, we found an extra heterochromatin on the long-arm interstitial region of chromosome 2R. This extra heterochromatin was polymorphic in the population. The plants with the extra heterochromatin showed a specific DNA fragment of 1.2 kb in digests prepared with the restriction enzyme DraI. The fragment was cloned and used as a probe for fluorescent in-situ hybridization (FISH). The clone, pScJNK1, showed a hybridization signal at the extra heterochromatic region. The segregation of the number of signals corresponded to the number of the extra heterochromatin of the 2R chromosome, indicating that the sequence might construct the heterochromatin. Southern hybridization using the clone as a probe showed a ladder pattern, suggesting that the sequence was a tandem repeat. Three sequences homologous to pScJNK1 were isolated; these were 1192–1232 bp, 44.7–45.9% in GC content, highly homologous (〉93%) with each other, and did not show any significant homology to other sequences in a DNA database. Slot blot hybridization using pScJNK1 as a probe indicated that there were about 4000 copies of the sequence in the haploid genome carrying the extra heterochromatin, whereas less than 20 copies existed in the genome without the heterochromatin. Southern hybridization using MspI and HapII indicated that all of the second cytosine nucleotides in CCGG sites in the sequence were methylated in the extra heterochromatin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009226612818
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  • 5
    Electronic Resource
    Electronic Resource
    A Novel Repetitive Sequence of Sugar Cane, SCEN Family, Locating on Centromeric Regions (1998)
    Springer
    Chromosome research 6 (1998), S. 295-302 
    Details
    ISSN: 1573-6849
    Keywords: CENP-B box ; FISH ; Saccharum species ; tandem repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tandem repetitive sequences consisting of 140-bp repetitive units were cloned from sugar cane genomic DNA and designated the SCEN family. In situ hybridization revealed that they were located on the centromeric region of almost all of the chromosomes of sugar cane. The 140-bp sequence included three CENP-B box-like sequences. Phylogenetic analysis of the members of the SCEN family revealed that the sequences had 75% homology with each other, on average, and that the sequences could not be further classified into smaller subfamilies. The copy number of the sequence was estimated to be 2.6 × 105 per haploid sugar cane genome and, therefore, 4.6 × 103 or 630 kb per chromosome on average.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009270824142
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  • 6
    Electronic Resource
    Electronic Resource
    Identification of RFLP markers linked with heading date and its heterosis in hexaploid wheat (2000)
    Springer
    Euphytica 116 (2000), S. 111-119 
    Details
    ISSN: 1573-5060
    Keywords: heading date ; heterosis ; linkage analysis ; photoperiod ; QTL ; vernalization requirement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Recombinant inbred lines (RILs) from a cross between hexaploid wheat (T. aestivum cv. Chinese Spring (CS) and T. spelta (Sp)) were used for RFLP analysis of heading date and heterosis. Fourteen RFLP markers linking with heading date were identified; two were localized on chromosome 1A, one on 2A, three on 2B, one on 2D, four on 5A, two on 7A and one unlinked but reported to be on group 2. All of these markers may be attributable to genes for earliness per se. However, the markers in the chromosomes of 1A and 7A are new to this study. RILs were crossed with (tim)-CS, the alloplasmic CS with T. timopheevi cytoplasm, and the heterosis from earlier-parent and mid-parents were calculated for the F1s to examine the heterotic effect toward earliness on heading date. Five and two RFLP markers were associated with heterosis from the earlier-parent and mid-parents, respectively. They were distributed on the chromosomes of homoeologous groups 1 and 2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004076826528
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  • 7
    Electronic Resource
    Electronic Resource
    Fertility restoration and nor suppression caused by Aegilops mutica chromosomes in alloplasmic hybrids and lines (1997)
    Springer
    Euphytica 94 (1997), S. 145-149 
    Details
    ISSN: 1573-5060
    Keywords: hybrid wheat ; fertility restoration ; Aegilops mutica ; Triticum aestivum ; powdery mildew ; NOR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract By crossing Aegilops mutica with Triticum dicoccum as a bridge species and backcrossing with common wheat as a recurrent pollen parent, male sterile alloplasmic line(s) were produced. In progeny of the crosses, a self fertile plant with 42 chromosomes was selected and named R 20. From this plant several lines that possessed Rf (fertility restoring) genes and/or powdery mildew resistant genes were obtained. Apparently, the system of sterility-fertility of pollen can be applied for hybrid wheat production. In addition, the disease resistance may be used in breeding. The male fertile lines possessed one or more Ae. mutica sat-chromosome(s), which show the ability to suppress the nucleolar organizing regions of chromosomes 1B and 6B of common wheat. The relation between the sat-chromosomes and male fertility restoration is not yet clear.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002955305001
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  • 8
    Electronic Resource
    Electronic Resource
    High-resolution cytological mapping of the long arm of chromosome 5A in common wheat using a series of deletion lines induced by gametocidal (Gc) genes of Aegilops speltoides (1994)
    Springer
    Molecular genetics and genomics 244 (1994), S. 253-259 
    Details
    ISSN: 1617-4623
    Keywords: Gametocidal genes ; Chromosome deletion ; Cytological map of common wheat ; DNA markers ; Structural analysis of chromosome rearrangements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gametocidal (Gc) genes of Aegilops in the background of the wheat genome lead to breakage of wheat chromosomes. The Q gene of wheat was used as a marker to select 19 deletion lines for the long arm of chromosome 5A of common wheat, Triticum aestivum cv. Chinese Spring (CS). The extents of deleted segments were cytologically estimated by the C-banding technique. The DNAs of deletion lines were hybridized with 22 DNA probes recognizing sites on the long arm of the chromosome (5AL) to determine their physical order. Based on the breeding behavior of the deletion lines, the location of a novel gene (Pv, pollen viability) affecting the viability of the male gamete was deduced. The segment translocated from 4AL to 5AL in CS was cytologically estimated to represent 13% of the total length of 5AL. Although DNA markers were almost randomly distributed along the chromosome arm, DNA markers located around the centromere and C-banded regions were obtained only rarely. Some deletion lines were highly rearranged in chromosome structure due to the effect(s) of the Gc gene. Applications of Gc genes for manipulating wheat chromosomes are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285452
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  • 9
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    Identification and Variation of Glutelin α Polypeptides in the Genus Oryza Assessed by Two-Dimensional Electrophoresis and Step-by-Step Immunodetection (2008)
    American Chemical Society
    Details
    Publication Date: 2008-07-01
    Print ISSN: 0021-8561
    Electronic ISSN: 1520-5118
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Published by American Chemical Society
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  • 10
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    Chromosome elimination by wide hybridization between Triticeae or oat plant and pearl millet: pearl millet chromosome dynamics in hybrid embryo cells (2010)
    Springer
    Details
    Publication Date: 2010-10-16
    Print ISSN: 0967-3849
    Electronic ISSN: 1573-6849
    Topics: Biology
    Published by Springer
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