Abstract
Two kinds of tandem repetitive sequences were isolated from Leymus racemosus (Lam.) Tzvelev. One of them was classified in the 350-bp family originally isolated from Secale. The other was a novel repetitive sequence family, named ‘TaiI family’, which consisted of a repeat unit of 570 bp. Fluorescence in-situ hybridization of the chromosomes of L. racemosus indicated that both families were located in subtelomeric heterochromatin and that the 350-bp family and TaiI family occupied different heterochromatin regions. In addition, even homologous chromosomes did not show the same patterns of TaiI and 350-bp families. The combination of these two families of repetitive sequences, together with Afa-family sequences and rDNAs, helps to identify the ten homologous chromosome pairs of L. racemosus. From these data, we proposed a karyotype of L. racemosus and compared it with other karyotypes already reported.
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Kishii, M., Nagaki*, K., Tsujimoto, H. et al. Exclusive Localization of Tandem Repetitive Sequences in Subtelomeric Heterochromatin Regions of Leymus Racemosus (Poaceae, Triticeae). Chromosome Res 7, 519–529 (1999). https://doi.org/10.1023/A:1009285311247
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DOI: https://doi.org/10.1023/A:1009285311247