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  • 1
    Signatur: S 97. 0506 (771)
    In: Forschungsbericht
    Materialart: Schriftenreihen ausleihbar
    Seiten: 15 Seiten , Illustrationen, Diagramme
    ISBN: 9783941721777
    Serie: DGMK Forschungsbericht 771
    Sprache: Englisch
    Anmerkung: Text englisch mit deutscher und englischer Zusammenfassung
    Standort: Kompaktmagazin unten
    Zweigbibliothek: GFZ Bibliothek
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2015-04-02
    Beschreibung: RNA-seq is a sensitive and accurate technique to compare steady-state levels of RNA between different cellular states. However, as it does not provide an account of transcriptional activity per se , other technologies are needed to more precisely determine acute transcriptional responses. Here, we have developed an easy, sensitive and accurate novel computational method, iRNA-seq , for genome-wide assessment of transcriptional activity based on analysis of intron coverage from total RNA-seq data. Comparison of the results derived from iRNA-seq analyses with parallel results derived using current methods for genome-wide determination of transcriptional activity, i.e. global run-on (GRO)-seq and RNA polymerase II (RNAPII) ChIP-seq, demonstrate that iRNA-seq provides similar results in terms of number of regulated genes and their fold change. However, unlike the current methods that are all very labor-intensive and demanding in terms of sample material and technologies, iRNA-seq is cheap and easy and requires very little sample material. In conclusion, iRNA-seq offers an attractive novel alternative to current methods for determination of changes in transcriptional activity at a genome-wide level.
    Schlagwort(e): Genomics
    Print ISSN: 0305-1048
    Digitale ISSN: 1362-4962
    Thema: Biologie
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Publikationsdatum: 2012-05-03
    Beschreibung: Here we present the first four dimensional (time and three dimensional space resolved) experiment on a strongly deformed geological material. Results show that even complicated microstructures with large continuous and discontinuous changes in crystallographic orientation can be resolved quantitatively. The details that can be resolved are unprecedented and therefore the presented technique promises to become influential in a wide range of geoscientific investigations. Grain and subgrain scale processes are fundamental to mineral deformation and associated Earth Dynamics, and time resolved observation of these processes is vital for establishing an in-depth understanding of the latter. However, until recently, in situ experiments were restricted to observations of two dimensional surfaces. We compared experimental results from two dynamic, in situ annealing experiments on a single halite crystal; a 2D experiment conducted inside the scanning electron microscope and a 3D X-ray diffraction experiment. This allowed us to evaluate the possible effects of the free surface on grain and subgrain processes. The extent to which surface effects cause experimental artifacts in 2D studies has long been questioned. Our study shows that, although the nature of recovery processes are the same, the area swept by subgrain boundaries is up to 5 times larger in the volume than observed on the surface. We suggest this discrepancy is due to enhanced drag force on subgrain boundaries by thermal surface grooving. Our results show that while it is problematic to derive absolute mobilities from 2D experiments, derived relative mobilities between boundaries with different misorientation angles can be used.
    Digitale ISSN: 1525-2027
    Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Publiziert von Wiley im Namen von American Geophysical Union (AGU).
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Publikationsdatum: 2011-09-14
    Beschreibung: We tested the influence of a photothrombotic lesion in somatosensory cortex on plasticity in the mouse visual system and the efficacy of anti-inflammatory treatment to rescue compromised learning. To challenge plasticity mechanisms, we induced monocular deprivation (MD) in 3-mo-old mice. In control animals, MD induced an increase of visual acuity of the open eye and an ocular dominance (OD) shift towards this eye. In contrast, after photothrombosis, there was neither an enhancement of visual acuity nor an OD-shift. However, OD-plasticity was present in the hemisphere contralateral to the lesion. Anti-inflammatory treatment restored sensory learning but not OD-plasticity, as did a 2-wk delay between photothrombosis and MD. We conclude that (i) both sensory learning and cortical plasticity are compromised in the surround of a cortical lesion; (ii) transient inflammation is responsible for impaired sensory learning, suggesting anti-inflammatory treatment as a useful adjuvant therapy to support rehabilitation following stroke; and (iii) OD-plasticity cannot be conceptualized solely as a local process because nonlocal influences are more important than previously assumed.
    Print ISSN: 0027-8424
    Digitale ISSN: 1091-6490
    Thema: Biologie , Medizin , Allgemeine Naturwissenschaft
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Publikationsdatum: 2018
    Beschreibung: 〈span〉〈div〉ABSTRACT〈/div〉The [MnO|SiO〈sub〉2〈/sub〉,Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉,FeO,MgO] balanced ratio (〈span〉i.e.〈/span〉 the isometric log-ratio of the MnO concentration relative to the concentration of SiO〈sub〉2〈/sub〉, Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉, FeO and MgO) of chlorite and of whole-rock composition is an effective discriminant between Mesozoic stratigraphic formations in the Magallanes Basin (Chile). The MnO content in chlorite is only controlled by the host rock chemistry and is dependent on the geological environment. The MnO content in chlorite remains unchanged at low-grade metamorphic conditions. Single-grain chlorite analysis (〈span〉n〈/span〉 = 1042, electron microprobe) and whole-rock analysis (〈span〉n〈/span〉 = 40, X-ray fluorescence) were used to discriminate stratigraphic formations and to decipher differences in the depositional environment in the Magallanes Basin. The samples are from one Upper Jurassic and three Cretaceous sedimentary units that were affected either by low-grade regional metamorphism or by Miocene contact metamorphism. The highest [MnO|SiO〈sub〉2〈/sub〉,Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉,FeO,MgO] values are recorded in the upper Zapata Formation. The Punta Barrosa, Cerro Toro and Tobífera Formations show slightly lower [MnO|SiO〈sub〉2〈/sub〉,Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉,FeO,MgO] values. Elevated [MnO|SiO〈sub〉2〈/sub〉,Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉,FeO,MgO] values at the transition between Zapata and Punta Barrosa Formations record an oxygenated shallow marine environment that can be linked to the closure of the Rocas Verdes Basin and the onset of fold-and-thrust belt formation. Decreasing [MnO|SiO〈sub〉2〈/sub〉,Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉,FeO,MgO] values from the Punta Barrosa towards the Cerro Toro Formation indicate gradually increasing water depths during the Upper Cretaceous that correlate well with the global sea level.〈/span〉
    Print ISSN: 0009-8558
    Digitale ISSN: 1471-8030
    Thema: Geologie und Paläontologie
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Publikationsdatum: 2017-07-22
    Beschreibung: We rigorously quantify the probability of liquid or ice thermodynamic phase using only shortwave spectral channels specific to the NASA MODIS, VIIRS, and the notional future PACE imager. The results show that two shortwave-infrared channels (2135 nm and 2250 nm) provide more information on cloud thermodynamic phase than either channel alone; in one case, the probability of ice phase retrieval increases from 65 to 82% by combining 2135 and 2250 nm channels. The analysis is performed with a nonlinear statistical estimation approach, the GEneralized Nonlinear Retrieval Analysis (GENRA). The GENRA technique has previously been used to quantify the retrieval of cloud optical properties from passive shortwave observations, for an assumed thermodynamic phase. Here we present the methodology needed to extend the utility of GENRA to a binary thermodynamic phase space (i.e. liquid or ice). We apply formal information content metrics to quantify our results; two of these (mutual and conditional information) have not previously been used in the field of cloud studies.
    Print ISSN: 0148-0227
    Thema: Geologie und Paläontologie , Physik
    Publiziert von Wiley im Namen von American Geophysical Union (AGU).
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Publikationsdatum: 2013-01-09
    Beschreibung: The coagulation protease activated protein C (aPC) confers cytoprotective effects in various in vitro and in vivo disease models, including diabetic nephropathy. The nephroprotective effect may be related to antioxidant effects of aPC. However, the mechanism through which aPC may convey these antioxidant effects and the functional relevance of these...
    Print ISSN: 0027-8424
    Digitale ISSN: 1091-6490
    Thema: Biologie , Medizin , Allgemeine Naturwissenschaft
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Publikationsdatum: 2005
    Beschreibung: Earthquake scarps associated with recent historical events have been found on the floor of the Sea of Marmara, along the North Anatolian Fault (NAF). The MAuto-Regressive Moving Average-processRASCARPS cruise using an unmanned submersible (ROV) provides direct observations to study the fine-scale morphology and geology of those scarps, their distribution, and geometry. The observations are consistent with the diversity of fault mechanisms and the fault segmentation within the north Marmara extensional step-over, between the strike-slip Ganos and Izmit faults. Smaller strike-slip segments and pull-apart basins alternate within the main step-over, commonly combining strike-slip and extension. Rapid sedimentation rates of 1-3 mm/yr appear to compete with normal faulting components of up to 6 mm/yr at the pull-apart margins. In spite of the fast sedimentation rates the submarine scarps are preserved and accumulate relief. Sets of youthful earthquake scarps extend offshore from the Ganos and Izmit faults on land into the Sea of Marmara. Our observations suggest that they correspond to the submarine ruptures of the 1999 Izmit (Mw 7.4) and the 1912 Ganos (Ms 7.4) earthquakes. While the 1999 rupture ends at the immediate eastern entrance of the extensional Cinarcik Basin, the 1912 rupture appears to have crossed the Ganos restraining bend into the Sea of Marmara floor for 60 km with a right-lateral slip of 5 m, ending in the Central Basin step-over. From the Gulf of Saros to Marmara the total 1912 rupture length is probably about 140 km, not 50 km as previously thought. The direct observations of submarine scarps in Marmara are critical to defining barriers that have arrested past earthquakes as well as defining a possible segmentation of the contemporary state of loading. Incorporating the submarine scarp evidence modifies substantially our understanding of the current state of loading along the NAF next to Istanbul. Coulomb stress modeling shows a zone of maximum loading with at least 4-5 m of slip deficit encompassing the strike-slip segment 70 km long between the Cinarcik and Central Basins. That segment alone would be capable of generating a large-magnitude earthquake (Mw 7.2). Other segments in Marmara appear less loaded. FROTH
    Schlagwort(e): Earthquake hazard ; Turkey ; Fault zone ; NAF ; G3 ; G-cubed ; AGU ; Ucarkus ; Lepinay ; Cagatay ; Cakir ; Structural geology ; 7230 ; Seismology: ; Seismicity ; and ; tectonics ; Oezalaybey ; Ozalaybey ; Lefevre ; 7223 ; Earthquake ; interaction, ; forecasting, ; and ; prediction ; morphology ; submersible ; 8110 ; Tectonophysics: ; Continental ; tectonics: ; general ; 1766 ; 1894 ; 1912 ; 1999 ; Earthquake
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Publikationsdatum: 2006
    Schlagwort(e): TF II ; Task Force II ; New tectonic causes of volcano failure
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Publikationsdatum: 2016-07-21
    Beschreibung: Bovine cytochrome c oxidase is an integral membrane protein complex comprising 13 protein subunits and associated lipids. Dimerization of the complex has been proposed; however, definitive evidence for the dimer is lacking. We used advanced mass spectrometry methods to investigate the oligomeric state of cytochrome c oxidase and the potential...
    Print ISSN: 0027-8424
    Digitale ISSN: 1091-6490
    Thema: Biologie , Medizin , Allgemeine Naturwissenschaft
    Standort Signatur Erwartet Verfügbarkeit
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