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  • 2015-2019  (237)
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  • 1
    Call number: S 97. 0506 (771)
    In: Forschungsbericht
    Type of Medium: Series available for loan
    Pages: 15 Seiten , Illustrationen, Diagramme
    ISBN: 9783941721777
    Series Statement: DGMK Forschungsbericht 771
    Language: English
    Note: Text englisch mit deutscher und englischer Zusammenfassung
    Location: Lower compact magazine
    Branch Library: GFZ Library
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  • 2
    Publication Date: 2015-04-02
    Description: RNA-seq is a sensitive and accurate technique to compare steady-state levels of RNA between different cellular states. However, as it does not provide an account of transcriptional activity per se , other technologies are needed to more precisely determine acute transcriptional responses. Here, we have developed an easy, sensitive and accurate novel computational method, iRNA-seq , for genome-wide assessment of transcriptional activity based on analysis of intron coverage from total RNA-seq data. Comparison of the results derived from iRNA-seq analyses with parallel results derived using current methods for genome-wide determination of transcriptional activity, i.e. global run-on (GRO)-seq and RNA polymerase II (RNAPII) ChIP-seq, demonstrate that iRNA-seq provides similar results in terms of number of regulated genes and their fold change. However, unlike the current methods that are all very labor-intensive and demanding in terms of sample material and technologies, iRNA-seq is cheap and easy and requires very little sample material. In conclusion, iRNA-seq offers an attractive novel alternative to current methods for determination of changes in transcriptional activity at a genome-wide level.
    Keywords: Genomics
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
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  • 3
    Publication Date: 2018
    Description: 〈span〉〈div〉ABSTRACT〈/div〉The [MnO|SiO〈sub〉2〈/sub〉,Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉,FeO,MgO] balanced ratio (〈span〉i.e.〈/span〉 the isometric log-ratio of the MnO concentration relative to the concentration of SiO〈sub〉2〈/sub〉, Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉, FeO and MgO) of chlorite and of whole-rock composition is an effective discriminant between Mesozoic stratigraphic formations in the Magallanes Basin (Chile). The MnO content in chlorite is only controlled by the host rock chemistry and is dependent on the geological environment. The MnO content in chlorite remains unchanged at low-grade metamorphic conditions. Single-grain chlorite analysis (〈span〉n〈/span〉 = 1042, electron microprobe) and whole-rock analysis (〈span〉n〈/span〉 = 40, X-ray fluorescence) were used to discriminate stratigraphic formations and to decipher differences in the depositional environment in the Magallanes Basin. The samples are from one Upper Jurassic and three Cretaceous sedimentary units that were affected either by low-grade regional metamorphism or by Miocene contact metamorphism. The highest [MnO|SiO〈sub〉2〈/sub〉,Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉,FeO,MgO] values are recorded in the upper Zapata Formation. The Punta Barrosa, Cerro Toro and Tobífera Formations show slightly lower [MnO|SiO〈sub〉2〈/sub〉,Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉,FeO,MgO] values. Elevated [MnO|SiO〈sub〉2〈/sub〉,Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉,FeO,MgO] values at the transition between Zapata and Punta Barrosa Formations record an oxygenated shallow marine environment that can be linked to the closure of the Rocas Verdes Basin and the onset of fold-and-thrust belt formation. Decreasing [MnO|SiO〈sub〉2〈/sub〉,Al〈sub〉2〈/sub〉O〈sub〉3〈/sub〉,FeO,MgO] values from the Punta Barrosa towards the Cerro Toro Formation indicate gradually increasing water depths during the Upper Cretaceous that correlate well with the global sea level.〈/span〉
    Print ISSN: 0009-8558
    Electronic ISSN: 1471-8030
    Topics: Geosciences
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  • 4
    Publication Date: 2017-07-22
    Description: We rigorously quantify the probability of liquid or ice thermodynamic phase using only shortwave spectral channels specific to the NASA MODIS, VIIRS, and the notional future PACE imager. The results show that two shortwave-infrared channels (2135 nm and 2250 nm) provide more information on cloud thermodynamic phase than either channel alone; in one case, the probability of ice phase retrieval increases from 65 to 82% by combining 2135 and 2250 nm channels. The analysis is performed with a nonlinear statistical estimation approach, the GEneralized Nonlinear Retrieval Analysis (GENRA). The GENRA technique has previously been used to quantify the retrieval of cloud optical properties from passive shortwave observations, for an assumed thermodynamic phase. Here we present the methodology needed to extend the utility of GENRA to a binary thermodynamic phase space (i.e. liquid or ice). We apply formal information content metrics to quantify our results; two of these (mutual and conditional information) have not previously been used in the field of cloud studies.
    Print ISSN: 0148-0227
    Topics: Geosciences , Physics
    Published by Wiley on behalf of American Geophysical Union (AGU).
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  • 5
    Publication Date: 2016-07-21
    Description: Bovine cytochrome c oxidase is an integral membrane protein complex comprising 13 protein subunits and associated lipids. Dimerization of the complex has been proposed; however, definitive evidence for the dimer is lacking. We used advanced mass spectrometry methods to investigate the oligomeric state of cytochrome c oxidase and the potential...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 6
    Publication Date: 2016-06-21
    Description: Limiting the public cost of stationary battery deployment by combining applications Nature Energy, Published online: 20 June 2016; doi:10.1038/nenergy.2016.79 Batteries are promising to support energy systems based on intermittent renewable energy, yet their investment attractiveness remains low. Using a techno-economic model, Stephan  et al.  find that returns can be increased and risks reduced if batteries are used for multiple stationary applications.
    Electronic ISSN: 2058-7546
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Springer Nature
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  • 7
    Publication Date: 2015-05-01
    Description: On 28 October 2012, an M w  7.8 earthquake occurred off the west coast of Haida Gwaii, British Columbia. Although past large events at this margin reflect strike-slip motion between the Pacific and North American plates (e.g., 1949 M s  8.1), this earthquake involved low-angle thrust faulting with a slip direction almost perpendicular to the margin, a consequence of slip partitioning at this obliquely convergent margin. To refine regional source models, we investigated the coseismic and postseismic displacements using a network of Global Positioning System (GPS) sites. Coseismic movement at the campaign site closest to the coast and about 30 km from the epicenter is 115 cm to the south-southwest, with 30 cm of subsidence. The only continuously recording GPS station on Haida Gwaii at the time of the earthquake, about 80 km from the epicenter, provides a robust coseismic displacement estimate of 22 cm to the south-southwest. The coseismic results are consistent with a shallow-dipping thrust rupture underlying the Queen Charlotte terrace, immediately seaward of the Queen Charlotte fault. These results allowed us to update rupture models that were originally derived from seismic waveforms and tsunami data. Estimates of cumulative postseismic horizontal displacements over about one year from seven sites are up to 6 cm, with a systematic along-strike variation in azimuth from south-southwest to southeast from north to south in the study area. Besides viscoelastic stress relaxation, these postseismic trends may indicate afterslip on the deeper plate interface beneath northern Moresby Island and possibly aseismic slip along the Queen Charlotte or subparallel faults offshore of southern Moresby Island. Online Material: Figures of three-component daily position estimates from Bernese analysis at Global Positioning System tracking stations on Haida Gwaii and the adjacent mainland.
    Print ISSN: 0037-1106
    Electronic ISSN: 1943-3573
    Topics: Geosciences , Physics
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  • 8
    Publication Date: 2015-05-01
    Description: We have studied transport characteristics of mesoscopic multiple-mode superconducting contacts formed between two grains in bulk two-gap magnesium diboride. The experimental setup was realized by driving a normal-metal tip into MgB 2 polycrystalline sample and proved to be extremely stable, providing possibility to perform pressure experiments at low temperatures. It is argued that in our procedure a small piece of the superconducting electrode is captured by the tip apex and, as a result, two junctions in series are formed: a junction between a tip and MgB 2 grain and a mesoscopic disordered contact between two superconducting pellets. Although the relative weight of the first junction resistance was considerably less, its contribution is shown to be important for the comparison of measured data with expected gap values. Two hallmarks of multiple Andreev reflections inside the MgB 2 –c–MgB 2 contact (c stands for a high-transparent constriction), a zero-bias 1/ | V | -like singularity of the dc differential conductance and peaks connected to the two gap values, have been revealed. Finally, we report results of a hydrostatic compression experiment showing the evolution of the MgB 2 gap values with pressure. In contrast to the theoretical expectations, we have observed an increase of the smaller gap Δ π whereas the larger gap Δ σ decreased with increasing pressure as it should be for the electron–phonon pairing mechanism. We argue that the so-called separable model of anisotropy effects is insufficient to describe such changes and only improved two-band versions are capable to reproduce the pressure effect on the energy gaps in magnesium diboride.
    Print ISSN: 1063-777X
    Electronic ISSN: 1090-6517
    Topics: Physics
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  • 9
    Publication Date: 2016-04-12
    Description: We have built, operated, and characterized a compact ion source for low-energy bunches of singly charged atomic ions in a vacuum beam line. It is based on atomic evaporation from an electrically heated oven and ionization by electron impact from a heated filament inside a grid-based ionization volume. An adjacent electrode arrangement is used for ion extraction and focusing by applying positive high-voltage pulses to the grid. The method is particularly suited for experimental environments which require low electromagnetic noise. It has proven simple yet reliable and has been used to produce μ s-bunches of up to 10 6 Mg + ions at a repetition rate of 1 Hz. We present the concept, setup and characterizing measurements. The instrument has been operated in the framework of the SpecTrap experiment at the HITRAP facility at GSI/FAIR to provide Mg + ions for sympathetic cooling of highly charged ions by laser-cooled 24 Mg + .
    Print ISSN: 0034-6748
    Electronic ISSN: 1089-7623
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
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  • 10
    Publication Date: 2015-12-25
    Description: To evaluate compaction and interstitial melt expulsion during cumulate formation, a 20 m cumulate section including the UG2 and UG3 chromitites from a 264 m drill core through the Upper Critical Zone of the Bushveld Complex (South Africa) has been studied. The cumulates in the studied section are as follows: 3 m plagioclase pyroxenite to pyroxenite, pegmatoid footwall pyroxenite at the lower contact to UG2, 0·7 m UG2 chromitite, 6·8 m pyroxenite, 0·24 m UG3 chromitite, 2·0 m plagioclase-rich pyroxenite changing locally to norite, the two 5 cm leader stringers UG3a and UG3b, and 7 m total of olivine pyroxenites grading into plagioclase pyroxenites. All pyroxenites are dominated by orthopyroxene (opx) and the cumulate sequence is topped by mottled anorthosite grading into norite. Stratigraphic concentrations of major and trace elements of 52 bulk-rock samples were determined. Bulk-rock Mg-numbers are 0·79–0·81 throughout the silicate cumulate units, and 0·40–0·46 in the chromitite layers. The stratigraphic distribution of six incompatible trace elements (K, Rb, Ba, Cs, Zr and Th) has been used to determine the amount of trapped liquid ( F TL ) or paleo-porosity in the cumulate rocks. Final porosities (volume fractions), based on averages from the six trace elements, are 0·06–0·33 in the pyroxenites. In chromitite layers, trapped melt fractions of 0·12–0·36 are calculated from incompatible trace element concentrations, but bulk SiO 2 concentrations and X-ray tomography yield 0·04–0·17 higher porosities. Hence, the bulk silicate fraction in the chromitites may not necessarily correspond to the trapped liquid fraction, as poikilitic opx was crystallizing while the silicate melt still equilibrated. Using a previously derived experiment-based model for compaction time scales, gravitationally driven chemical compaction in the UG2–UG3–pyroxenite section is calculated to occur within 1–10 years. This time frame corresponds to the times necessary to cool a 20 m layer by 10–50°C, the temperature interval argued to encompass the liquidus and almost complete solidification. Compaction within a decade can in fact easily develop the paleo-porosities indirectly observed today and is probably stopped by crystallization of the interstitial liquid. Contrary to previous assertions, melt expulsion from the cumulate pile does not hinder compaction; calculated permeabilities would allow for the migration of an order of magnitude higher amount of melt than has to be expelled from the 20 m pile of cumulate. The pegmatoid zones in the chromitite footwalls enriched in incompatible trace elements are consistent with a collection of interstitial melts expelling from the underlying compacting pyroxenites. Their entrapment below the chromitite layers suggests that these act as permeability barriers. This is in part due to their finer grain size compared with the pyroxenites, but is mainly due to the crystallization of large poikilitic opx during compaction.
    Print ISSN: 0022-3530
    Electronic ISSN: 1460-2415
    Topics: Geosciences
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