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  • 1
    Digitale Medien
    Digitale Medien
    High resolution bands in maize chromosomes by G-banding methods (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 265-272 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Maize ; Chromosome banding ; G-banding method ; Enzymatic maceration ; Image analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary It was demonstrated that G-bands are unequivocally present in plant chromosomes, in contrast to what had been formerly believed by plant cytologists. Maize chromosomes prepared by an enzymatic maceration method and treated with trypsin or SDS showed clear G-bands spreading along the chromosomes. The most critical point during the G-banding procedures was the post-fixation with glutaraldehyde solution. Banding patterns were processed by using the chromosome image analyzing system and a clearer image was obtained. Gbanding technique and the image manipulation method described here can be applied to many plant species, and would contribute new information in the field of plant cytology and genetics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224397
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  • 2
    Digitale Medien
    Digitale Medien
    Influence of Cementite and Graphite on Grain Growth and Texture Development in High Carbon Sheet Steel (1992)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 94-96 (Jan. 1992), p. 443-448 
    Details
    ISSN: 1662-9752
    Quelle: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Thema: Maschinenbau
    Materialart: Digitale Medien
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/21/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.94-96.443.pdf
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  • 3
    Digitale Medien
    Digitale Medien
    Molecular cloning of the S-layer protein gene of Campylobacter rectus ATCC 33238 (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 116 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Abstract A genomic DNA library of Campylobacter rectus (Wolinella recta) ATCC 33238 was constructed using bacteriophage lambda EMBL3 as a vector. One clone expressing the S-layer protein was identified immunologically with the antiserum to the S-layer protein of C. rectus ATCC 33238. Western immunoblotting using monoclonal antibodies directed against the S-layer protein showed a single blot of recombinant protein at 150 kDa, suggesting that this clone contained the entire coding region of the S-layer protein gene. Additionally, the S-layer protein gene was subcloned into plasmid vector pUC18. Southern hybridization revealed that the S-layer protein gene was present on the chromosome of C. rectus as a single-copy gene, and that there were minor heterogeneities among the S-layer protein genes of clinical isolates. Moreover, a spontaneous stable mutant strain, which has no S-layer expression, also conserved the full-length gene of the S-layer protein.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06668.x
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  • 4
    Digitale Medien
    Digitale Medien
    Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans (1990)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 69 (1990), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Abstract Three colonial variants of Actinobacillus actinomycetemcomitans, which formed transparent rough (TR)-, transparent smooth (TS)-, and opaque smooth (OS)-surfaced colonies, were described in relation to their fimbriation. TR- and TS-cells were adhesive to agar and glass surfaces but not the OS-cells. The examination by electron microscopy revealed that TR-cells were highly fimbriated but not TS- and OS-cells. Thus, TS-cells seemed to be an intermediate type. The fimbriae were isolated from TR-cells by suspending in 0.15 M ethanolamine-HCl buffer (pH 10.5) and purified by dissolving non-fimbrial components in 0.5% deoxycholate and 0.7% n-octyl-β-d-glucopyranoside. The relative molecular mass of the fimbrial subunit protein was 54 000.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1990.tb04167.x
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  • 5
    Digitale Medien
    Digitale Medien
    Antigenic properties of Campylobacter rectus (Wolinella recta) major S-layer proteins (1993)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 108 (1993), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Abstract The antigenic properties of the surface layer (S-layer) proteins of various Campylobacter rectus strains including 24 clinical isolates and the type strain ATCC 33238 were examined. S-layer proteins were extracted from whole cells by acid treatment according to the method of McCoy et al. (Infect. Immun. 11, 517–525, 1975). The acid extracts from 23 of the isolates and ATCC 33238 contained two major proteins with molecular masses of 130 kDa and 150 kDa, both of which were identified as subunits of the S-layer after comparison with the protein profiles of acid-treated (S-layer-deficient) cells. An S-layer protein from one isolate (CI-808) demonstrated a different molecular mass (160 kDa). Both the 150-kDa proteins of ATCC 33238 and isolate CI-306 and the 160-kDa protein of CI-808 were purified by ion-exchange chromatography in the presence of urea. In Ouchterlony immunodiffusion experiments with these purified proteins and rabbit antiserum raised to each purified protein, both common and strain-specific antigenic determinants were identified in the C. rectus S-layer proteins.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1993.tb06115.x
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  • 6
    Digitale Medien
    Digitale Medien
    Synchrotron radiation-excited etching of SiO2 with SF6 at 143 and 251 A(ring) using undulator radiation (1990)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 56 (1990), S. 2189-2191 
    Details
    ISSN: 1077-3118
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: Photoexcited etching of SiO2 surface with SF6 gas is studied using undulator radiation at 143 and 251 A(ring) as an extreme ultraviolet light source. The SF6 pressure and the wavelength dependences of the etch rates have been measured for SiO2 in the pressure region between 0.016 and 0.50 Torr. We find that, at these wavelengths, the etch rate is proportional to the intensity of the light absorbed by the surface species, most probably SiO2 in the pressure region studied.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.103241
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  • 7
    Digitale Medien
    Digitale Medien
    Fluid dynamically caused cycle-to-cycle variations in piston-driven pipe expansion flows (1992)
    Springer
    Experiments in fluids 13 (1992), S. 1-10 
    Details
    ISSN: 1432-1114
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau
    Notizen: Abstract A piston-driven pipe flow with a sudden expansion in cross-sectional area was studied experimentally using flow visualization and laser-Doppler anemometry as basic experimental techniques. To drive the flow, a piston was moved in the larger of the two pipes. It drew in the fluid into the pipe assembly during the upward stroke and removed fluid during the downward stroke. The time-varying flow field was investigated, yielding detailed information on the flow structure, its development, and variation with time. In the present paper, particular attention is given to those flow conditions that resulted in cycle-to-cylce variations of the flow field. For some of the conditions, repetitive cycle-to-cycle variations of the flow field occurred, yielding identical flows for even-numbered flow cycles and also for odd numbered cycles. However, the latter differed from the former. The presented data show that non-repetitive cycle-to-cycle variations of the flow field also exist and that these can be driven into a state where the flow appears to be nearly chaotic. The results of the investigation clearly demonstrate that fluid dynamically caused cycle-to-cycle flow variations can exist in piston-driven internal flows with separation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00208068
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  • 8
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of prostaglandin endoperoxide synthase in the bovine intestine (1993)
    Springer
    Histochemistry and cell biology 99 (1993), S. 485-490 
    Details
    ISSN: 1432-119X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The localization of prostaglandin (PG) endoperoxide synthase in bovine intestine was examined immunocytochemically with polyclonal antibody raised against PG endoperoxide synthase purified from bovine seminal glands. The most intense positive staining reaction for the enzyme was present in mast cells. Mast cells were found to be widely distributed in the intestinal wall, and were particularly numerous in the lamina propria. Most of the mast cells in the lamina propria of the intestinal villi were elongated and oriented with their long axis parallel to the plane of the absorptive epithelium. In whole mount preparations of jejunal villi, mast cells were seen to form a two-dimensional network in the lamina propria. In addition to mast cells, smooth muscle cells of the inner circular muscle layer and muscularis mucosae, nerve cells and fibers, endothelial cells of arterioles, and serosal epithelial cells also showed faint to moderate staining for the enzyme. These results suggested that mast cells are the major source of PGs in the bovine intestinal wall. The characteristic arrangement of mast cells in the intestinal villi may be related to their functions in this portion of the bovine intestine.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00274102
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  • 9
    Digitale Medien
    Digitale Medien
    A diurnal variation of vasoactive intestinal peptide (VIP) mRNA under a daily light-dark cycle in the rat suprachiasmatic nucleus (1991)
    Springer
    Histochemistry and cell biology 95 (1991), S. 525-528 
    Details
    ISSN: 1432-119X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary We detected a diurnal change of vasoactive intestinal peptide (VIP) messenger RNA (mRNA) in the rat suprachiasmatic nucleus (SCN) using the in situ hybridization technique combined with computed image analysis. The amount of VIP mRNA was greatest at 02.00 h, the next largest level was seen at 20.00 h during the dark phase and smallest at 14.00 h during the light phase. We also confirmed that the onset of the fluctuation of mRNA occurred 2–6 h prior to the diurnal variation of the content of VIP-like immunoreactivity by semiquantitative immunocytochemistry. These findings suggest that light stimulus from the retina is a primarily influence on the transcription of VIP mRNA and induces a diurnal variation of VIP synthesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00315750
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  • 10
    Digitale Medien
    Digitale Medien
    Somatic chromosome map of rice by imaging methods (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 589-596 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Rice ; Chromosome map ; Ideogram ; Image analysis ; Condensation pattern
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Rice somatic chromosomes were completely identified and quantitatively mapped based on an image parameter, condensation pattern (CP), or a chromosomal density profile determined by imaging methods. The CP corresponds to the compactness of the chromatin fibers along the chromatid, which is characteristic in small plant chromosomes such as rice chromosomes at the mitotic pro-metaphase stage. The standard CP for every chromosome was obtained by averaging 60 CPs from 30 chromosome spreads. Each standard CP exhibited a characteristic pattern of the chromosome, which enabled it to be distinguished from the other chromosomes. An ideogram based on the numerical data and the standard CP was established. The chromosomal address was also determined based on the degree of condensation, and the fractional length of each chromosomal address was quantitatively presented.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226723
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