Skip to main content
Log in

Somatic chromosome map of rice by imaging methods

  • Originals
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

Rice somatic chromosomes were completely identified and quantitatively mapped based on an image parameter, condensation pattern (CP), or a chromosomal density profile determined by imaging methods. The CP corresponds to the compactness of the chromatin fibers along the chromatid, which is characteristic in small plant chromosomes such as rice chromosomes at the mitotic pro-metaphase stage. The standard CP for every chromosome was obtained by averaging 60 CPs from 30 chromosome spreads. Each standard CP exhibited a characteristic pattern of the chromosome, which enabled it to be distinguished from the other chromosomes. An ideogram based on the numerical data and the standard CP was established. The chromosomal address was also determined based on the degree of condensation, and the fractional length of each chromosomal address was quantitatively presented.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Chen JT, Lai HC, Hwang YH, Chung MC, Wu HK (1982) Identification of rice reciprocal translocation and the location of lazy gene. Bot Bull Acad Sin 23:71–87

    Google Scholar 

  • Chung MC, Wu HK (1987) Karyotype analysis of IR36 and two trisomic lines of rice. Bot Bull Acad Sin 28:289–304

    Google Scholar 

  • Comings (1978) Mechanisms of chromosome banding and implications for chromosome structure. Annu Rev Genet 12:25–46

    Google Scholar 

  • Fukui K (1985) Identification of plant chromosomes by image analysis method. The Cell (Tokyo) 17:145–149

    Google Scholar 

  • Fukui K (1986a Comparison between Giemsa and orcein staining methods in rice chromosomes. La Kromosomo II-43–44:1398–1404

    Google Scholar 

  • Fukui K (1986b) Standardization of karyotyping plant chromosomes by a newly developed chromosome image analyzing system (CHIAS). Theor Appl Genet 72:27–32

    Google Scholar 

  • Fukui K (1988) Analysis and utility of chromosome information by using the chromosome image analyzing system, CHIAS. Bull Natl Inst Agrobiol Resour 4:153–176

    Google Scholar 

  • Fukui K (1989) Application of image analysis methods in plant chromosome research. In: Hong Deyuan (ed) Plant chromosome research 1987. Academia Sinica, Beijing, pp 195–200

    Google Scholar 

  • Fukui K, Ito K (1989) Computer simulation of the chromosome image of barley. Bull Natl Inst Agrobiol Resour 5:43–57

    Google Scholar 

  • Fukui K, Mukai Y (1988) Condensation pattern as a new image parameter for identification of small chromosomes in plants. Jpn J Genet 63:359–366

    Google Scholar 

  • Fukui K, Kakeda K, Iijima K, Ishiki K (1988) Computer-aided identification of rice chromosomes. Rice Genet Newslett 5:31–34

    Google Scholar 

  • Hu CH (1958) Karyological studies in haploid rice II. Analysis of karyotype and somatic pairing. Jpn J Genet 33:296–301

    Google Scholar 

  • Iijima K, Fukui K (1991) Investigation on the conditions for the image analysis of plant chromosomes. Bull Natl Inst Agrobiol Resour 6:1–60

    Google Scholar 

  • Ishii K, Mitsukuri S (1960) Chromosome studies on Oryza. I. Somatic chromosomes of Oryza sativa L. Bull Res Coll Agric Vet Med Nihon Univ 2:44–53

    Google Scholar 

  • Kamisugi Y, Fukui K (1990) Automatic karyotyping of plant chromosomes by imaging technique. BioTechniques 8:290–295

    Google Scholar 

  • Khan SH (1975) A technique for staining rice chromosomes. Cytologia 40:595–598

    Google Scholar 

  • Khush GS, Singh RJ, Sur SC, Librojo AL (1984) Primary trisomies of rice: origin, morphology, cytology, and use in linkage mapping. Genetics 107:141–163

    Google Scholar 

  • Kurata N, Omura T (1978) Karyotype analysis in rice 1. A new method for identifying all chromosome pairs. Jpn J Genet 53:251–255

    Google Scholar 

  • Kurata N, Iwata N, Omura T (1981a) Karyotype analysis in rice 2. Identification of extra chromosomes in trisomic plants and banding structure on some chromosomes. Jpn J Genet 56:41–50

    Google Scholar 

  • Kurata N, Omura T, Iwata N (1981b) Studies on centromere, chromomere, and nucleolus in pachytene nuclei of rice, Oryza sativa, microsporocytes. Cytologia 46:791–800

    Google Scholar 

  • Kuwada Y (1910) A cytological study of Oryza sativa L. Bot Mag Tokyo 26:267–281

    Google Scholar 

  • Merrit JF, Burus JA (1974) Chromosome banding in Nicotiana otophora without denaturation and renaturation. J Hered 65:101–103

    Google Scholar 

  • Murata M, Orton TJ (1984) G-band-like differentiation in mitotic pro-metaphase chromosome of celery. J Hered 75:225–228

    Google Scholar 

  • Nandi HK (1936) The chromosome morphology, secondary association, and origin of cultivated rice. Jpn J Genet 33:315–336

    Google Scholar 

  • Oka H, Wu HK (1988) Comparison of data on rice chromosomes presented by different authors. Rice Genet Newslett 5:34–41

    Google Scholar 

  • Shastry SVS, Ranga Rao DR, Mistra RN (1960) Pachytene analysis in Oryza, I. Chromosome morphology in Oryza sativa. Indian J Genet Plant Breed 20:15–21

    Google Scholar 

  • Wu HK, Chung MC, Chen MH (1985) Karyotype analysis of cultivar IR36. Rice Genet Newslett 2:54–57

    Google Scholar 

  • Yanagisawa T, Tano S, Fukui K, Harada K (1990) Maker chromosomes commonly observed in the genus Glycine. Theor Appl Genet 81:606–612

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by F. Mechelke

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fukui, K., Iijima, K. Somatic chromosome map of rice by imaging methods. Theoret. Appl. Genetics 81, 589–596 (1991). https://doi.org/10.1007/BF00226723

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00226723

Key words

Navigation