ISSN:
1432-2242
Keywords:
Fluorescence in-situ hybridization (FISH)
;
Ribosomal DNA
;
Genus Oryza
;
Image analysis
;
NOR variability
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The 17s-5.8s-25s ribosomal RNA gene (rDNA) loci in Oryza spp. were identified by the fluorescence in-situ hybridization (FISH) method. The rDNA loci were located on one-to-three chromosomes (two-to-six sites) within the eight diploid Oryza spp. One of the rDNA loci gave the weakest hybridization signal. This locus is reported for the first time in the genus Oryza. The chromosomes containing the rDNA loci were determined to be numbers 9, 10 and 11 in descending order of the copy number of rDNA. The application of image analysis methods, after slide preparation treatments (post-treatments), and the use of a thermal cycler, greatly improved the reproducibility of the results. The evolutionary significance of the variability of rDNA loci among the Oryza spp. is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00225782
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