Summary
It was demonstrated that G-bands are unequivocally present in plant chromosomes, in contrast to what had been formerly believed by plant cytologists. Maize chromosomes prepared by an enzymatic maceration method and treated with trypsin or SDS showed clear G-bands spreading along the chromosomes. The most critical point during the G-banding procedures was the post-fixation with glutaraldehyde solution. Banding patterns were processed by using the chromosome image analyzing system and a clearer image was obtained. Gbanding technique and the image manipulation method described here can be applied to many plant species, and would contribute new information in the field of plant cytology and genetics.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aguiar-Perecin DEMLR, Vosa CG (1985) C-banding in maize. II. Identification of somatic chromosomes. Heredity 54:37–42
Anderson LK, Stack SM, Mitchell JB (1982) An investigation of the basis of a current hypothesis for the lack of G-banding in plant chromosomes. Exp Cell Res 138:433–436
Bennett MD, Heslop-Harrison JS, Smith JB, Ward JP (1983) DNA density in mitotic and meiotic metaphase chromosomes of plants and animals. J Cell Sci 63:173–179
Burkholder GD, Duczek LL (1982) The effect of chromosome banding techniques on the proteins of isolated chromosomes. Chromosoma 87:425–435
Chen R (1988) G-band staining of chromosomes in some higher plants with special reference to Lilium davidii. La Kromosomo II-50:1635–1651
Chen R, An Z, Song W, Li X, Su J (1986) A preliminary study on the G-bands of chromosomes in some plants. J Wuhan Bot Res 4:111–118
Chow C, Larter EN (1981) Centromeric banding in maize. Can J Genet Cytol 23:255–258
Chughtai SR, Steffensen DM (1987) Knob composition of commercial inbred lines. Maize Genet Coop Newslett 61:96–98
Cortés F, Escalza P (1986) Analysis of different banding patterns and late replicating regions in chromosomes of Allium cepa, A. sativum, and A. nigrum. Genetica 71:39–46
Döbel P, Schubert I, Rieger R (1978) Distribution of heterochromatin in a reconstructed karyotype of Vicia faba as identified by banding-and DNA-late replication patterns. Chromosoma 69:193–209
Drewry A (1982) G-banded chromosomes in Pinus resinosa. J Hered 73:305–306
Endo TR (1986) Complete identification of common wheat chromosomes by means of the C-banding technique. Jpn J Genet 61:89–93
Fukui K (1985) Identification of plant chromosomes by image analysis method. The Cell (Tokyo) 17:145–149
Fukui K (1986) Standardization of karyotyping plant chromosomes by a newly developed chromosome image analyzing system (CHIAS). Theor Appl Genet 72:27–32
Fukui K (1988) Analysis and utility of chromosome information by using the chromosome image analyzing system, CHIAS. Bull Natl Inst Agrobiol Resour 4:153–176
Fukui K, Kakeda K, Ito K (1986) Analysis and utility of chromosome information. 14. High resolutional G-like-bands in Atriplex patula. In: Proc 37th Ann Meet Soc Chromo Res Tokyo, p 24
Fukui K, Fukui K, Kakeda K, Ito K (1989) Quantitative analysis of the C-, N-, and G-banded plant chromosomes by the application of imaging techniques. In: Hong DY (ed) Plant chromosome research 1987. Academia Sinica, Beijing, pp 195–200
Goldman MA, Holmquist GP, Gray MC, Caston LA, Nag A (1984) Replication timing of genes and middle repetitive sequences. Science 224:686–692
Greilhuber J (1977) Why plant chromosomes do not show Gbands. Theor Appl Genet 50:121–124
Greilhuber J (1988) “Self-tanning” — a new and important source of stoichiometric error in cytophotometric determination of nuclear DNA content in plants. Plant Syst Evol 158:87–96
Holmquist G, Gray M, Porter T, Jordan J (1982) Characterization of Giemsa dark- and ligh-band DNA. Cell 31:121–129
Jagiello G, Fang JS (1980) A pachytene map of the mouse oocyte. Chromosoma 77:113–121
Korenberg JR, Rykowski MC (1988) Human genome organization: Alu, Lines, and the molecular structure of metaphase chromosome bands. Cell 53:391–400
Kurata N, Iwata N, Omura T (1981) Karyotype analysis in rice. II. Identification of extra chromosomes in trisomic plants and banding structure on some chromosomes. Jpn J Genet 56:41–50
McAlpine PJ, Van Cong N, Boucheix C, Pakstis AJ, Doute RC, Shows TB (1987) The 1987 catalog of mapped genes and report of the nomenclature committee. Cytogenet Cell Genet 46:29–101
Murata M, Orton TJ (1984) G-band-like differentiation in mitotic prometaphase chromosomes of celery. J Hered 75:225–228
Ohnuki Y (1968) Structure of chromosomes. I. Morphological studies of the spiral structure of human somatic chromosomes. Chromosoma 25:402–428
Rattner JB, Lin CC (1985) Radial loops and helical coils coexist in metaphase chromosomes. Cell 42:291–296
Schweizer D (1973) Differential staining of plant chromosomes with Giemsa. Chromosoma 40:307–320
Schweizer D (1976) Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58:307–324
Schweizer D (1981) Counterstain-enhanced chromosome banding. Hum Genet 57:1–14
Sumner AT, Evans HJ, Buckland RA (1971) New technique for distinguishing between human chromosomes. Nature New Biol 232:31–32
Takayama S (1976) Configurational changes in chromatids from helical to banded structures. Chromosoma 56:47–54
Wang HC, Kao KN (1988) G-banding in plant chromosomes. Genome 30:48–51
Yang XF, Zhang ZL (1988) In situ induction of G-bands and macrocoils in plant chromosomes. Hereditas 109:45–51
Zhu F, Wei J, Fu J, Li L (1986) High resolution banding in chromosomes of Triticum monococum. In: Proc 1st Int Symp chromosome Eng Plants, Xian, pp 226–227
Author information
Authors and Affiliations
Additional information
Communicated by Hu Han
Rights and permissions
About this article
Cite this article
Kakeda, K., Yamagata, H., Fukui, K. et al. High resolution bands in maize chromosomes by G-banding methods. Theoret. Appl. Genetics 80, 265–272 (1990). https://doi.org/10.1007/BF00224397
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00224397