ISSN:
1432-2242
Keywords:
Maize
;
Chromosome banding
;
G-banding method
;
Enzymatic maceration
;
Image analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary It was demonstrated that G-bands are unequivocally present in plant chromosomes, in contrast to what had been formerly believed by plant cytologists. Maize chromosomes prepared by an enzymatic maceration method and treated with trypsin or SDS showed clear G-bands spreading along the chromosomes. The most critical point during the G-banding procedures was the post-fixation with glutaraldehyde solution. Banding patterns were processed by using the chromosome image analyzing system and a clearer image was obtained. Gbanding technique and the image manipulation method described here can be applied to many plant species, and would contribute new information in the field of plant cytology and genetics.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00224397
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