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  • 1
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    A study on methanol herbal plant extract of Echinacea (Echinacea purpurea) on immunity level of Mugil cephalus (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publikationsdatum: 2021-05-19
    Beschreibung: Medicinal plants have been used in oriental medicine for centuries. Echinacea purpurea (Asteraceae), also known as the purple coneflower, is an herbal medicine with positive effects on various immune parameters that has been used customarily as a treatment for the common cold, coughs, bronchitis, upper respiratory infections, and some inflammatory conditions. The aim of this study was to investigate the effect of Echinacea purpurea (EP) extract on a non-specific immunity of Mugil cephalus. Three hundred and sixty Gray mullet larvae with average weight of 0.75 ± 0.02 g and an average length of 4.40 ±0.81cm collected from Ramin port where is located at 5 km far from the Chabahar and finally transferred to lab of trial in Offshore Research Center. This research was designed based on 4 treatment, Each with 3 replicates. The major factors examined containing growth factors mainly consists of average weight, average daily gain, protein efficiency rate, protein productive rate, hematological and biochemical factors including globin, albumin, total protein, lysozyme, ultimately, immunological factors, and respiratory burst and phagocytosis percent. In general, the results of this study showed that the addition of 100 and 200 mg of EP extract per kg of diet led to a significant increase in growth parameters (final weight, food intake, daily growth rate and efficiency of protein), hematology (RBC, WBC, Hb, Hct) and biochemical factors (total protein, albumin, globulin) in gray mullet were compared to control. The highest lysozyme activity, immunoglobulin, phagocytosis and respiratory burst was observed in treatment containing 200 mg EP extract per kg food. Treatment containing 200 mg EP extract per kg food showed a minimal mortality after challenge with bacteria photobacterium damselae compared to the control treatment. Finally, the present results suggest that diet containing 200 200 mg EP extract per kg food could improve growth, hematology, immunity and resistance against photobacteriosis of grey mullet.
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Echinacea purpurea ; Mugil cephalus ; Photobacteriosis ; Growth ; Gematology ; Immunity ; Photobacterium damselae ; Medicinal ; EP
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 35pp.
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  • 2
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    Investigstion on Effect prepared vaccine by Y-ray on production rate and resistance to white spot disease in Shrimp Litopenaeus vannamei (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publikationsdatum: 2021-05-19
    Beschreibung: White Spot Disease (WSD) is a important disease due to economic impacts in shrimp industries. Spreading of this disease in shrimp farms can caused a 100% mortality during 3-10 days. Therefore control of this disease is a strategy in shrimp industry. Vaccination is a way to control of WSD. In several years ago during a project several type vaccine of this virus by association of Atomic Energy Organization is produced that among of them the virus inactivated by GAMA radiation had better results in laboratory. To test of this vaccine in field, a research pilot was carried out. Initially 20000 shrimp napliies were obtained from one of commercial hatchery in Bushehr province, then devided to two groups vaccinated and unvaccinated. The vaccinated group also devided . 26and PL12 , group B vaccinated at PL15and PL 5to two groups, group A that vaccinated at postlarva e (PL) Rrsults showed that difference of growth performance and survival rate between vaccinated and unvaccinated without challenge with WSV after 80 days is not significantly (P〈0.05). but survival rate in vaccinated groups after challenge with WSV was significantly (P〈0.05) further the unvaccinated group. Also the results showed survival rate of group B after challenge with WSV is better than group A and this difference was significantly (P〈0.05). this study showed vaccination of shrimp postlarvae with GAMA radiation vaccine can control of shrimp mortality in incidence of WSD in farms.
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Litopenaeus vannamei ; WSSV ; Gamma radiation ; Vaccination ; Y-ray ; White spot disease ; Shrimp ; Nauplii ; Growth ; Survival rate ; Mortality
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 65pp.
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  • 3
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    An investigation on the use of Prosopis juliflora pods as a carbohydrate source supplemented with probiotics in the diet of Labeo rohita fingerlings (2018)
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    Publikationsdatum: 2021-05-19
    Beschreibung: The present study investigated the use of fermented Prosopis juliflora pods (PJP) as a carbohydrate source alternative to rice bran (RB), and a P. juliflora pods based diet formulation supplemented with probiotics Lactobacillus acidophilus (LA), Lactobacillus bulgaricus 009 (LB) and Streptococcus thermophilus (ST) on the growth of Labeorohita (rohu) fingerlings. The diets were isonitrogenous (39%) and isoenergetic (15.9 KJg-1 gross energy); and were prepared using rice bran (as reference diet RD), crude pods (PJPC), fermented pods (PJPF) and with probiotics L. acidophilus (PJPLA), L. bulgaricus (PJPLB) and S. thermophilus (PJPST). The results showed that fermentation of P. juliflora pods with L. acidophilus significantly increased (p〈0.05) protein, lipid, Ca, Fe, Mg, K and reducing sugar; and completely eliminated phytic acid. The diets PJPF and PJPLA produced significantly higher (p〈0.05) percent weight gain, SGR, FCR and PER as compared to PJPC, PJPLB and PJPST diets; and achieved growth performance comparable to RD. An improvement (p〈0.05) in the carcass protein has been detected in groups PJPF and PJPLA in comparison to RD, PJPC, PJPLB and PJPST. The L. acidophilus was found to be effective in controlling coliform count in gut as compared to L. bulgaricus and S. thermophilus; and also in controlling total viable count, total coliform count and fecal streptococci count in fecal matter. The results indicate that PJPF and PJPLA deserve further investigations as alternative feed for rohu.
    Beschreibung: Published
    Schlagwort(e): Prosopi sjuliflora pods ; Antinutritional factors ; Probiotics ; Fermentation ; Growth ; Microflora ; Diet ; Fingerlings ; Prosopis juliflora ; Lactobacillus acidophilus ; Lactobacillus bulgaricus ; Streptococcus thermophilus
    Repository-Name: AquaDocs
    Materialart: Journal Contribution , Refereed
    Format: pp.327-345
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  • 4
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    Several fish species carp culture with Chinese black carp to increase diversity in warm water fish farms (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
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    Publikationsdatum: 2021-05-19
    Beschreibung: The objective of this study was to investigate the effects of black carp introduction in chines carp poly culture as well as its effect on the growth, survival rate and production of chines carp species. This study conducted in two consecutive year. The period of culture in first year was during Jun 1 until Des 5 ,2009 and in second year it was from Jun 4 to Dec, 1,2010 .The experiment were started by selecting 9 earth end pond each with 800 m2 area and was performed in triplicate with 2 treatment and one control , each year .The ponds were treated by liming and fertilizer before filling water and then stocked with 3000 chines carp ( 140 Silver carp , 42 Grass carp , 42 Common carp and 25 Bighead carp ) while the number of Black carp in treatments 1, 2, 3 and 4, respectively, 250, 500, 75 and 125 pieces in Ha, blackcap was added to the control species respectively . The number of fish species harvested at end of culture in treatment 1, 2 and control .The total fish species harvested at the end of first year was 2276 out 1904 species released. The number of fishes catch in treatment 1,2 and control were 637,658and 573 species. the statistical analysis showed that average weight of black carp were 407±150 gr in treatment 1, 234±121gr in treatment 2 with the minimum and maximum weight of 242 and 780 gr in first treatment as well as 110 and 750 gr in second treatment the production of black carp in first treatment were 42 kilogram per hectare and 60 kilogram in second treatment. The growth rate were higher in second treatment than first one. In second year the investigation repeated in triplicate with treatment3, 4 and one control .The total stocking density in treatment 3,4 and control were 665,633, and 574 pieces. Statistical analysis showed that total weight of black carp in third treatment was2049 ±614gr and in forth treatment was 2361 ±60 gr where the minimum and maximum values were 3115 gr and 1025 gr respectively. The survival rat of black carp in treatment3 higher than in were treatment 4 while the growth in treatment 4 was higher treatment3.The total production of black carp in treatment 3 and 4 were 123 and 181kilogram per hectare respectively. The ANOVA test conducted for grass carp revealed that treatment 4 is significantly difference with treatment 3 and control. While for silver carp the treatment 4 is significantly difference with treatment 1 and control while for Big head carp the treatment 4 was significantly difference with control at 5% level (P〈 %5) .On second year the black carp reached to acceptable market size which could be compatible with other carp such as common carp and grass carp comparative analysis between different stocking density indicate that the stocking density with 125 pieces showed the highest positive effects.
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Fish ; Species ; Carp culture ; Chinese black carp ; Diversity ; Warm water ; Growth ; Survival rate ; Silver Carp ; Grass carp ; Common carp ; Bighead carp ; ANOVA
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 111pp.
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  • 5
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    The study of possible biotechniques and normative to production some species of Anostraca (Phallocryptus spinosa) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publikationsdatum: 2021-05-19
    Beschreibung: This project was carried out in order to determine the hatching conditions and laboratory culture methods of Fairy Shrimps ( Phallocryptus spinosa). Physico- chemical factors and phytoplankton of natural habitat of this organism was investigated in Khasellou region around Azarshahr – East Azarbaijan province. The results showed that occurance and growth of fairy shrimps in their habitates were beginning from early Aprill and their survival was decreased with increasing temperature, precipitation and increasing the salinity of their culture medium. Then they disappeared. Their habitat phytoplankton contained 3 phylla and 10 genera, from which green algae enjoyed the greatest density in May. The pool salinity was around 20-17 g/l , when metanauplii was observed in early spring. In the pool containing and without fairy shrimps the oxygen concenteration was high (9 mg/l) and low (2.9 mg/l ) , respectively . In 9 prawn ornamental fish genera, feeding with fairy shrimp showed a significant higher fecundity and hatching percentage compaired to manual diets (cow liver, spinach). In all groups fed with fairy shrimp the duration of spawning time were reduced from 15 to 8-10 days and the color was enhanced. The means of cyst number per captured female also, cyst , decapsulated cyst and Naupilli diameters were mesured 142.9±19.0 cysts , 273.2 ± 4.9 µ , 242.4± 3.8 µ and 542.6 ± 27.0 µ., respectively . The hatching rate was increased by reducing temperature and salinity of their culture mediums from 25 to 15 ◦ C , and 28 to 18 g/l, respectively.The highest hatching was observed on 5 th day at 15 ◦ C and 18 g/l (5, 33 %). In addition, low but multiple hatching was observed. Therefore , water temperature and salinity affected hatching rate in this species.The hatching success of Phallocryptus spinosa cysts was beter when collecting cysts together with dry sediments of their habitat without separation sensetive cysts . In addition to cold keeping and freezing, the hydration – dehydration method caused more diapause deactivation for hatching induction. The laboratory results revealed that the survival of fairy shrimps were highest at 15 ◦ C (42%) and lowest at 25◦ C (26%), respectively. Also, their life was inhibited in 30◦ C.In different salinities, the mean survival rate were 26% (Salinity = 15 g/l) and 20% (Salinity = 25 g/l), respectively. The mean survival percentage of Phallocryptus spinosa cultured in natural habitat water (64.3%) was higher than aerated tap water (23, 3 %). Also, feeding with Nanochlorepsis caused higher survival rates. On the other hand, Beakers yeast caused higher mortality rates. Therefore , regarding to relatively high sensitivity of these species to physico – chemical and nutritional conditions of their culture medium, it is better to culture these species with expantion of their natural habitat in order to preserve their biodiversity , culture and increase stocking density with regard to environmental considerations .
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Physico-chemical ; Environmental ; Fairy Shrimps ; Phallocryptus spinosa ; Hatching ; Culture ; Biotechnique ; Species ; Anostraca ; Phytoplankton ; Growth ; Temperature ; Salinity ; Phylla ; Feeding ; Survival rate ; Mortality ; Biodiversity
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 104pp.
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  • 6
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    Effects of seawater pH on survival, growth, energy budget and oxidative stress parameters of juvenile turbot Scophthalmus maximus (2018)
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    Publikationsdatum: 2021-05-19
    Beschreibung: This study aimed to elucidate the influence of environment pH on survival, growth, energy allocation and oxidative damage of juvenile Scophthalmus maximus (19.89±0.25 g). Six pH treatments (6.3±0.2, 6.8±0.2, 7.3±0.2, 7.8±0.2, 8.3±0.2, 8.8±0.2) lasting for eight weeks were included. Measurements of survival (SR), feed conversion ratio (FCR), specific growth rate (SGR), weight gain rate (WGR), energy allocation, liver superoxide dismutase activity (SOD), catalase activity (CAT), and malondialdehyde concentration (MDA) were done. Results show that SR did not vary from pH 6.3 to 7.8, but reduced then significantly (p〈0.05); FCR raised remarkably (p〈0.05) while SGR and WGR decreased pronouncedly (p〈0.05). Most of the food energy was used in metabolism, followed by growth, feces loss and nitrogenous excretion. Energy deposited for growth showed a decreasing tendency when pH raised; while for metabolism showed a reverse trend. SOD showed insignificant difference from pH 6.3 to 7.8, but the activities then elevated obviously (p〈0.05) though a slight decrease was found from pH 8.3 to 8.8 (p〉0.05). CAT kept stable between pH 6.3 and 7.3, then sharply increased (p〈0.05), but no significant differences were found between pH 8.3 and 8.8 (p〉0.05). Subsequent decrease in MDA was found from pH 6.3 to 8.3 then the concentration smoothly increased. Overall, our results indicate that a pH in the range of 6.8 to 7.8 is recommended in the growth environment in cultivation of juvenile turbot.
    Beschreibung: Published
    Schlagwort(e): Juvenile turbot ; Scophthalmus maximus ; pH ; Water ; Survival ; Growth ; Energy allocation ; Oxidative damage ; Stress ; Parameters ; FCR ; SGR ; WGR ; CAT ; SOD
    Repository-Name: AquaDocs
    Materialart: Journal Contribution , Refereed
    Format: pp.675-689
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  • 7
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    Age, growth and reproduction of Chondrostoma regium (Heckel, 1843) from the Zayandeh Roud River, Iran (2018)
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    Publikationsdatum: 2021-05-19
    Beschreibung: Age, growth and reproduction were investigated for Chondrostoma regium, collected monthly (November 2007- August 2008) from the Dimeh Spring region, Zayandeh Roud River, Iran. The maximum age of collected female and male fish were estimated based on scale increments as five and four years respectively. Von Bertalanffy growth equations were calculated as L_t=246[1-e^(-0.206(t-0.034))], W_t=163.29〖[1-e^(-0.206(t-0.034))]〗^2.77 for females and L_t=253.1[1-e^(-0.206(t-0.175))], W_t=188.25〖[1-e^(-0.206(t-0.175))]〗^2.99 for males. Size ranges of the male and female specimens were almost similar. The fork length range for females was 10.5-18.0 cm and 10.7-19.7 cm for males and the weight range was 15.7-85.4 g in females, and 14.5-88.5 g in males. The female-male ratio was 1.8:1 (p〈0.05), and for both sexes gonadal development and sexual maturity occurred in the second year of their life. Length-weight relationships revealed isometric growth pattern for males and a negative allometric growth for females. Monthly data of Gonadosomatic Index, and ovary condition suggested that spawning occurred during May and June. The mean absolute and relative fecundity was calculated as 2429±1512 (eggs/female) and 78.118.7 (egg/g), respectively, and fecundity was significantly correlated with both body weight (r2=0.90) and fork length and (r2=0.84).
    Beschreibung: Published
    Schlagwort(e): Biology ; Physiology ; Reproduction ; Chondrostoma regium ; Fecundity ; Length-weight relationships ; Age ; Growth
    Repository-Name: AquaDocs
    Materialart: Journal Contribution , Refereed
    Format: pp.810-822
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  • 8
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    Effect of lactic acid bacteria and the potential probiotic Hafnia alvei on growth and survival rates of narrow clawed crayfish (Astacus leptodactylus Esch., 1823) stage II juveniles (2018)
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    Publikationsdatum: 2021-05-19
    Beschreibung: The aim of this study was to screen potential probiotic bacteria against Aeromonas hydrophila and determine the effects of antagonistic bacteria and a commercial product containing lactic acid bacteria on the survival and growth of stage II Astacus leptodactylus juveniles. For this purpose, a total of 110 bacterial strains were isolated from adult, stage II crayfish juveniles and rearing water screened for antagonistic activities against A. hydrophila with well diffusion agar assay. Hafnia alvei strain from stage II crayfish juveniles displayed the inhibition zone (10mm) against A. hydrophila. The experiment was conducted in a completely randomized design with four treatments for 60 days: (I) crayfish fed with live food without probiotics (control group); (II) crayfish fed with live food enriched with lactic acid bacteria (0.015 gL-1); (III) crayfish fed with live food enriched with Hafnia alvei (106 CFU mL−1); (IV) crayfish fed with control diet and H. alvei added to rearing water (106 CFU mL−1). As a result of this study, lactic acid bacteria and Hafnia alvei applications did not positively affect growth and survival of stage II A. leptodactylus juveniles. In the future, studies on screening potential probiotic bacteria should be used in vitro and in vivo tests. In addition, it will be useful to investigate the lactic acid bacteria and Bacillus spp. from indigenous microflora of crayfish.
    Beschreibung: Published
    Schlagwort(e): Aquaculture ; Crayfish ; Astacus leptodactylus ; Aeromonas hydrophila ; Lactic acid bacteria ; Hafnia alvei ; Growth ; Survival ; Juveniles ; Fish
    Repository-Name: AquaDocs
    Materialart: Journal Contribution , Refereed
    Format: pp. 1307-1317
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  • 9
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    The effect of salinity on morphological and molecular characters and physiological responses of Nostoc sp. ISC 101 (2018)
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    Publikationsdatum: 2021-05-19
    Beschreibung: Taking into account the high potential of cyanobacteria to tolerate salinity stress, researches have evaluated the morphological and physiological behavior of these microorganisms in recent years. This study is conducted to investigate the impact of different concentrations of NaCl on the morphological and physiological traits of Nostoc sp. ISC 101. Biometrical and morphological observations are carried out by light and scanning electron microscopy. Results indicated that vegetative cells and heterocysts were wider in control treatment in comparison with samples under different amounts of salinity. Akinete formation began in 3% NaCl and reached to highest level in 5%. The relative degeneration of structure of the cells in 5% salt was demonstrated. According to physiological impresses of salt it was found that growth rate decreased with increasing salinity. Total chlorophyll content stimulated in 1% salinity, but in the higher concentration it decreased vice versa. The rate of APC, PE, PC increased in 1% salinity, although in high level concentration they would be diminished. Photosynthesis rate was also decreased with increasing salinity but it was stimulated slightly in 1% NaCl. All in consequence, despite of acclimation of this strain to marine environment, not much tolerance was seen in the mentioned treatments, and increasing salinity to upper than 1% NaCl had destructive effects, and cyanobacterium maintained its growth rate at slightly saline environments.
    Beschreibung: Published
    Schlagwort(e): Biology ; Physiology ; Growth ; Morphology ; Nostoc ; Photosynthesis ; Salinity ; SEM ; 16S rRNA ; Morphological ; Physiological
    Repository-Name: AquaDocs
    Materialart: Journal Contribution , Refereed
    Format: pp.907-917
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  • 10
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    Bacteria effects (probiotics) on growth and survival rate and resistance of shrimp during propagation stages (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
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    Publikationsdatum: 2021-05-19
    Beschreibung: This project investigates bacteria effects as probiotics on growth and survival rate and resistance of shrimp during propagation stages. Bacterial flora were extracted from seawater , water of cultured farms , shrimp cultured and sludge from private propagation farms different bacteria such as Vibrio alginolyticus(serotype1-4) Vibrio splendidus I , Vibrio fluvialis ,Vibrio anguillarum ,Vibrio costicul, Vibrio vulnificus,Vibrio nereis,Vibrio camplbelli,Vibrio natriegens,Vibrio proteolyticus, Vibrio plegius ,Vibrio fischeri,Aeromonas hydrophila, Aeromonas schuberti,Aeromonas salmonisida, Aeromonas veronii , Pseudomonas pseudoalcaligenes, Pseudomonas chloraphis, Alcaligenes faecalis, Bacillus megaterium, Bacillus pumilus, Bacillus circulans, Bacillus cereus, Bacillus subtilis, Bacillus licheniformis, Lactobacillus case, Lactobacillus bulgaricus, Lactobacillus delbrukii, Lactobacillus fermentum, Lactobacillus leichmanii, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus lactis, Pediococcus acidilactici were isolated , identified and lyophilized.These bacteria in different experiments were used on shrimp life cycle stages and live food such as Chaetoceros , Skeletonema and Tetraselmis algae. Some serotypes of one bacterial species showed various reactions in media and experiment probiotics. Vibrio alginolyticus(serotype1) with 107 cells/ml can increase Tetraselmis sp production during 6 days to 71% compared to the control experiment without bacteria. Also Vibrio alginolyticus(serotype4) with 105 cells/ml can increase Tetraselmis spp production during 6 days to 389% more than control. Vibrio splendidus could increase 23% shrimp fork length in postlarvae 3 compared control. Vibrio alginolyticus+ Vibrio fischeri can increase survival , fork lenght and body weight of green tiger shrimp postlarvae significantly(P〈.05).
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Bacteria ; Shrimp ; Algae ; Probiotic ; Vibrio alginolyticus ; Vibrio splendidus ; Vibrio fluvialis ; Vibrio anguillarum ; Vibrio costicul ; Growth ; Survival rate ; Resistance
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 85pp.
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