Publication Date:
2002-11-16
Description:
Recently, we showed that antibodies catalyze the generation of hydrogen peroxide (H2O2) from singlet molecular oxygen (1O2*) and water. Here, we show that this process can lead to efficient killing of bacteria, regardless of the antigen specificity of the antibody. H2O2 production by antibodies alone was found to be not sufficient for bacterial killing. Our studies suggested that the antibody-catalyzed water-oxidation pathway produced an additional molecular species with a chemical signature similar to that of ozone. This species is also generated during the oxidative burst of activated human neutrophils and during inflammation. These observations suggest that alternative pathways may exist for biological killing of bacteria that are mediated by potent oxidants previously unknown to biology.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wentworth, Paul Jr -- McDunn, Jonathan E -- Wentworth, Anita D -- Takeuchi, Cindy -- Nieva, Jorge -- Jones, Teresa -- Bautista, Cristina -- Ruedi, Julie M -- Gutierrez, Abel -- Janda, Kim D -- Babior, Bernard M -- Eschenmoser, Albert -- Lerner, Richard A -- 5T32AI07606/AI/NIAID NIH HHS/ -- GM43858/GM/NIGMS NIH HHS/ -- P01CA27489/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2002 Dec 13;298(5601):2195-9. Epub 2002 Nov 14.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12434011" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies, Catalytic/immunology/*metabolism
;
Arthus Reaction/*immunology/metabolism
;
Blood Bactericidal Activity
;
Catalase/metabolism
;
Catalysis
;
Escherichia coli/*immunology
;
Hematoporphyrins/metabolism
;
Humans
;
Hydrogen Peroxide/metabolism
;
Indigo Carmine/metabolism
;
Inflammation/*immunology/metabolism
;
Mice
;
Neutrophil Activation
;
Neutrophils/immunology/*metabolism
;
Oxidation-Reduction
;
Ozone/*metabolism
;
Rabbits
;
Rats
;
Rats, Sprague-Dawley
;
Respiratory Burst
;
Singlet Oxygen/metabolism
;
Ultraviolet Rays
;
Water/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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