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  • Wiley  (233)
  • Iranian Fisheries Science Research Institute  (50)
  • American Association for the Advancement of Science (AAAS)
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  • 2020-2023  (2)
  • 2020-2022  (282)
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  • 1
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    Effect of Echinacea purpurea and Astragalus glycyphyllos on immune responses of rainbow trout (O. mykiss) and resistance to sterptococcosis (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25451 | 18721 | 2018-09-23 17:53:51 | 25451 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Nowadays, medical plants extract is used in aquaculture industry to enhance growth, immune system and fish resistance to different pathogens. In this study, some non-specific immune responses, hematology and biochemical parameters in rainbow trout juveniles (16 gr mean weight) and their resistance to Streptococcus iniae were evaluated following oral administration of Echinacea purpurea and Astragalus sp. extract. Three concentrations (0.5, 1 and 1.5 gr/kg of feed) of Echinacea purpurea and three concentrations (2, 3 and 5 gr/kg of feed) of Astragalus sp extract and control group were used for 60 days. Parameters evaluated included: levels of C4, C3 (complement components), free oxygen radicals (respiratory burst), Lysozyme, numbers of lymphocytes, monocytes and neutrophils. In the end, the relative survival rate (RSR) of fish was evaluated against S. iniae. The results showed that the levels of C3, lysozyme, oxygen free radicals, the number of neutrophils and lymphocytes in the experiment groups (the highest dose) compared to control group were increased significantly (p 〈0/05). While, the values of C4 and number of monocytes compared to the control group were not significantly different (p〉 0.05). The relative survival rates of fish following challenge with Streptococcus iniae, were 91/11, 93/33 and 44/44 percent in experiments (Echinacea purpurea and Astragalus sp.) and control groups, respectively. In conclusion, it may be state that Echinacea purpurea and Astragalus sp. extracts enhance the non-specific immune system and fish resistance against Streptococcusis, and it seems it can be used as immunostimulant in feed.
    Keywords: Aquaculture ; Iran ; Echinacea purpurea ; Astragalus sp ; Rainbow trout ; Streptococcusis ; Immune system ; Astragalus glycyphyllos ; O.mykiss ; Aquaculture ; Growth ; Juvenile ; Lysozyme ; Survival rate
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 106
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  • 2
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    The role of daphnia fed with purified and enriched phytoplankton with PUFA in enhancing growth and survival of Acipenser persicus larvae (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25464 | 18721 | 2018-09-26 09:35:11 | 25464 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: In this study, Daphnia longispina were fed with two freshwater green algae species, Chlorella vulgaris and Scenedesmus dimorphus, enriched with Docosahexaenoic acid (DHA). Chlorella vulgaris and Scenedesmus dimorphus were isolated and cultured for mass production. Three treatments with three replicates and a control group were considered to conduct this study. In order to investigate feeding, a total of 30 Acipenser persicus larvae with the initial mean weight of 68±3.6 mg were distributed into 12 plastic tanks of 60 L. Experimental fish larvae were fed daphnia collected from earthen ponds (as control group), daphnia enriched with Scenedesmus dimorphus (treatment 1), daphnia enriched with Chlorella vulgaris (treatment 2) and daphnia enriched with Chlorella vulgaris and Scenedesmus dimorphus (at the rates of 50%) (treatment 3). The microalgae density to enrich daphnia is estimated at 5×107 cells mL^-1. Fish larvae were fed at an amount of 30% body weight per day for four times. During the experimental period, water temperature, dissolved oxygen concentration and pH ranged between 18-24°C, 5.8-7.2 mg l^-1 and 5.6-8.2, respectively. Monounsaturated fatty acids (MUFA) were the highest (32.3 %) in D. longispina enriched with Chlorella vulgaris and the highest concentration of polyunsaturated fatty acids (PUFA) (19.8%) was observed in D. longispina enriched with Scenedesmus dimorphus. The highest (21.6%) and lowest (13.2%) concentrations of PUFA in fish larvae were recorded in treatment 3 and the control group, respectively. The results obtained from the body weight increase (BWI %) revealed that there were significant differences (P≤0.05) between treatment 3 (daphnia enriched with chlorella and scenedesmus) and other treatments. The highest (5.5±1.24% day^-1) and the lowest (4.6±1.13% day^-1) mean specific growth rates (SGR) were recorded in fish fed treatment 3 and the control group, respectively. The lowest (68%) and the highest (85%) survival rates were obtained in the control group and treatment 3, respectively.
    Keywords: Aquaculture ; Iran ; Daphnia ; Daphnia longispina ; Chlorella vulgaris ; Scenedesmus dimorphus ; Enrichment ; Microalgae ; Acipenser persicus ; survival ; Larvae ; Phytoplankton ; PUFA ; Growth
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 41
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  • 3
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    The use of lactic acid bacteria metabolites (nisin and organic acids) for the shelf life extension of trout (Oncorhynchus mykiss) and their effect on dominant bacteria in inoculation study (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25650 | 18721 | 2018-10-14 02:41:46 | 25650 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Nisin is a natural antimicrobial and has inhibitory effect on the pathogens and spoilage organisms. The efficiency of nisin reduced after reaction with food compounds. There is the hypothesis that combination of nisin with organic salt increases the shelf life of fish. The purpose of this study was mainly to evaluate the effects of nisin (0.15 %) and sodium acetate ( 1% ) individually and in combination on shelf life of fillets and whole trout ( without viscera). then, the behavior of Listeria monocytogenes and Clostridium botulinum type E were evaluated in different time at 40C. Physical and chemical parameters including: pH, Peroxide Value (PV), Tiobarbithoric Acid (TBA) and Total Volatile Nitrogen (TVN) and bacterial factors such as Total Viable Count (TVC), Pscycrothophic Count (PTC) and Lactic Acid Bacteria (LAB) were examined. These parameters were done at intervals of 4 days for 16 days. Results showed that chemical and bacterial factors of whole trout (Oncorhynchus mykiss) (without viscera) have been more favorable than the fillets . It also showed that the peroxide value, the thiobarbituric acid, volatile nitrogen bases, and pH in combination of sodium acetate and nisin Z treatments were exposed to significantly compared to control treatments (no preservatives) (P〈0.05). Treatments with sodium acetate and nisin Z (individual) were exist after combination treatment. Hence it can be concluded that the maximum shelf life of in preservative treatments was 16 days, but control treatment was 12 days in some cases and in some cases 16 days. Effects of treatments on Listeria monocytogenes and Clostridium botulinum in the control treatment showed the highest growth was observed in both species (log 8) but in single and combined treatment of bacterial growth slower , but is still the trend the 16th day of so Listeria and Clostridium log 5 to log 6, respectively. Vegetative cells of Clostridium were more sensitive in compare to Listeria. When using preservatives (chemical or biological) in fish tissue, parameters such as the type of preservative, used method, pH, proteolytic enzymes, and the incidence of resistant strains and so on influence of antimicrobial agents used. The overall conclusion of the study was shelf life of whole trout (without viscera) was more than fillets, combination of nisin and sodium acetate were better than other treatment and finally higher doses of bilogival preservatives are need to for achieve to significant reduction of pathogenic bacteria.
    Keywords: Fisheries ; Iran ; Nisin Z ; Sodium acetate ; Rainbow trout ; Listeria ; Monocytogenes ; Clostridium botulinum ; Lactic Acid ; Bacteria ; Organic acids ; Oncorhynchus mykiss ; Pathogen
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 94
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  • 4
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    Peptone production from marine and culture wastes by commercial enzymes for bacterial culture media (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25648 | 18721 | 2018-10-08 07:22:08 | 25648 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Thirty to 40% of total fish catch is converted to waste. Using different methods of hydrolysis of the protein can be recovered of fish waste and increase the amount of protein efficiency. In this study, the four enzymes Alcalase, protamex, pepsin and trypsin were used for hydrolysis of four fish species including common carp (Cyprinus carpio), silver carp (Hypophthalmichthys molitrix), grass carp (Ctenopharyngodon idella) and the Big head (Hypophthalmichthys nobilis). The effects of pH, temperature and hydrolysis time on the rate of hydrolysis were studied on soluble proteins and degree of hydrolysis (phase I). In the second step, proximate factors of peptone been evaluated and eventually replace commercial peptone media MRS (Lactobacillus plantarum, Lactobacillus delberuki, Lactobacillus casei, Streptococcus thermophilus) and TSB (Listeria monocytogenes, two species of Bacillus and Pseudomonas, Streptococcus faecium) and the optical density of bacteria at different times were compared with control samples. Results showed that the highest degree of hydrolysis and soluble proteins were referred to alcalase and protamex, pepsin and trypsin respectively. The highest value of hydrolysis, in all treatments, was attributed to grass carp and silver carp, common carp and big head respectively. The best pH and temperature for alcalase, protamex, pepsin and trypsin 8.5and 55, 7.5 and 55, 3.5, 37, 7 and 37 respectively. Best time to achieve the highest degree of hydrolysis and soluble protein was 90 minutes. Qualitative analysis showed that the highest and lowest amounts of protein and fat in the treatment of alcalase (about 70 % protein and less than 0.5 % fat) and protamex, pepsin and trypsin was then. The results of bacteria culture showed that the highest percentage growth of lactic acid bacteria was referred to Streptococcus thermophilus and Lactobacillus casei had the lowest rate of growth. In other bacteria, Pseudomonas and Bacillus species were the highest percentage of growth and Listeria monocytogenes and Streptococcus faecium respectively. In all treatments, alcalase had the best results and the peptone prepared from fish waste grass carp had the best condition for growth of used bacteria. It seems that the initial substrate , the parameters used such as temperature, pH, and enzyme hydrolysis time , have a significant effect on the quality of peptone and protein content in the final product is determined value of protein for culture of bacteria.
    Keywords: Biology ; Iran ; Warm water ; Protease enzymes ; Bacteria ; Degree of hydrolysis ; Soluble proteins ; Fish waste ; Peptone ; Marine ; Culture ; Alcalase ; Protamex ; Pepsin ; Common carp ; Cyprinus carpio ; Silver Carp ; Hypophthalmichthys molitrix ; Grass carp ; Ctenopharyngodon idella ; Big head ; Hypophthalmichthys nobilis
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 92
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  • 5
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    The comprehensive study of possibility ecological control Mnemiopsis leidyi in Caspian Sea activity: The study and recognization parasitic fauna and Bacterial flore in ctenophore (Mnemiopsis leidyi and Bereo ovata) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25134 | 18721 | 2018-08-26 13:35:47 | 25134 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Bacterial flora and parasitic fauna of M. leidyi an exotic invader jelly fish to Caspian Sea ecosystem and B. ovata to Black sea an alternative biological control agent was studied. During summer 1382 to spring 1383, using routine Bacteriological work. 72 sample of sea water Caspian Sea obtained from depth 20 and 50 meters, 36 sample of M. leidyi from depth 20 meters, 10 sample of B. ovata and 3 sample of sea water (Black sea) were collected and according to Bacteriological was studied. 216 sample of M. leidyi from depth 10 to 50 meters of Caspian Sea and 47 sample of B. ovata from Black and Marmarreh Sea (Turkey) were collected and was studied. In this study no parasite from was identified in M. leidyi (Caspian sea) but 64 percentage and 73 percentage of B. ovata (Marmarreh and Black sea respectively) contaminated to Trichodina ctenophore at varians concentration B. ovata of Black sea (130 min 1050 max) and B. ovata Marmarre sea (420 min 2100 max). While B. ovata kept at high salinity of 21 ppt was more contaminated with this pretrichial protozoan (Trichodina) than in low salinity (12/5 ppt). in comparision of bacterial flore in two cetenophore (M. leidyi and B. ovata) was observed that some of bacteria such as micrococcus sp, Aeromonas sp. Bacillus coagulans in both ctenophore and some other bacteria such as Agromobacterium and chromobacterium only observed in B. ovata but other researcher have reported fram Caspin sea and some of bacteria to specific Shewanella , Vibrio harveiy and bacillus linens was observed in B. ovata . Of course specific bacteria cannot transfer to Caspian Sea (different of salinity black sea (2/1%) to Caspian Sea (1/25 %)). Therefore if B. ovata to introduce to south Caspian Sea for biological control population M. leidyi. it is necessary at first some of viral pathogen in aquatic animal (fish) such as VNN, IPN,IHN,VHS,SVC was studied and then with confidence 95% non-infestation B. ovata to viruses and pass from bath anti parasite and anti-bacterial must be introduce to south Caspian sea.
    Keywords: Ecology ; Health ; Iran ; Caspian Sea ; Black sea ; Parasite ; Bacteria ; Beroe ovata ; Mnemiopsis leidyi ; Agromobacterium ; Chromobacterium
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 55
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  • 6
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    Feasibility study to develop fish culture sturgeons in the coastal line in Guilan and Mazandaran fishing ground (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25407 | 18721 | 2018-09-23 16:00:17 | 25407 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Keywords: Aquaculture ; Iran ; Guilan ; Mazandaran ; Feasibility ; Fish culture ; Sturgeons ; Coastal line ; Fishing
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 423
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  • 7
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    Single cell protein production from culture and marine fish wastes (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25159 | 18721 | 2018-08-26 17:13:41 | 25159 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The alarming rate of population growth has increased the demand for food production in third-world countries leading to a yawning gap in demand and supply. This has led to an increase in the number of hungry and chronically malnourished people. This situation has created a demand for the formulation of innovative and alternative proteinaceous food sources. Single cell protein production is a major step in this direction. SCP is the protein extracted from cultivated microbial biomass. Algae, fungi and bacteria are the chief sources of microbial protein that can be utilized as SCP. Produced proteins from these microbes have various nutrition values. SCP is the manufacture of cell mass using microorganisms by culturing on available agriculture, industrial wastes and fisheries by products. Fish wastes due to high protein are the most important substrates for SCP production. In this study, SCP production was done from Silver carp and tuna fish wastes (head, tail and vise versa) and cooked water of canned tuna factories. The used microbes were six genus and species of yeasts include Candida utilis, Saccharomyces cereviceae, Rhodotorula, Khyveromyces marxians, Zygosaccharomyces rouxii and Bacillus subtilis and B.licheniformis. The examination was done in bench scale and CSTR bioreactor (Continuous Stirred Tank Reactor). The effects of various parameters such as pH, temperature, time, supplemented substrates, method of inoculation of microbes, rpm were evaluated. Changes of microbial growth and protein contents were tested by using Optical Density (OD) and Makrokjeldal methods respectively. In end of examination, produced protein were extracted and lyophilized. The results showed that protein percentage in bacterial protein was than yeast protein but wet percentage in bacterial protein was low. Production value produced from tuna fish wastes was higher than (30-45 g/l) to Silver carp wastes (25-29 g/l) and cooked water (10-15 g/l). By adding supplemented substrates, production value has been increased. Candida utilis, in comparison other yeasts, has high activation. B.licheniformis has also had more activation than Bacillus subtilis. The results of the effect some parameters on fermentation showed that yeasts and Bacillus in pH= 5.4 and 32oC and pH=6.9 and 35oC were better than growth pH=6 and 25oC and pH=6.5 and 30oC respectively. Time of fermentation in batch and bioreactor was 54 and 21 hours respectively. High rpm has been caused increasing of microbial growth in bioreactor. The conclusion showed that with optimizing of the growth condition such as some parameters (pH, temperature, substrates and so on) produced SCP with high efficiency. However, produced SCP should be exanimated with other specific tests such as amino acid and fatty acid profiles, minerals, nucleic acids and so on. After full examination, this SCP as probiotic could be used in fish and poultry feed.
    Keywords: Biology ; Iran ; Single cell protein ; Marine ; Culture ; Fish wastes ; Yeast ; Bacterial ; Population ; Growth ; Biomass ; Algae ; Fungi ; Nutrition ; Candida utilis ; Silver carp ; Bacillus subtilis ; B.licheniformis ; pH ; Protein
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 132
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  • 8
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    Production of fish cheeses from silver carp by acid coagulation (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25162 | 18721 | 2018-08-26 17:33:11 | 25162 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Fish and fishery products have high nutrition value particularly omega-3 fatty acids. It is expected that the estimated production in 2030 will be 150 to 160 million tones but estimated demand will be 183 million tons. Processing of fish especially (culture fish) is one of the way attentions of people to fish and fishery products. Fish Finger, Fish Berger, Cracker, Fish Ball are the most important of fishery products. In this research was studied production of fish cheese from Silver carp (Hypophthalmichthys molitrix) from sarcoplasmic proteins. After primary preparation of fish (beheading, cutting, filleting and deboning) minced fish was washed with salt solution (0.01, and 0.04 mol) and were isolated sarcoplasmic proteins. Acetic acid (%5) and centrifuge were used for sedimentation and extraction of these proteins. Fifteen formulas were worked for preparing of fish cheese. Minced fish, sedimented protein and fish cheese were examinated for proximal parameters (protein, ash. moisture, carbohydrate and lipid) and microbial and chemical spoilage factors (Total Viable Count, Mold and Yeast, Total Volatile Nitrogen, and Peroxide Value). The shelf life and organoleptic characterization of fish cheese were also surveyed. The results indicated that mean of protein, lipid, ash, moisture and carbohydrate in fish cheese were %14/86, %28/01, %3/46, %48/27, %4/78 respectively. The best formula was treatment of 13 with %18/26 of protein. TVN and PV, in fish cheese, were at standard level but after 30 days stored in 4o C, PV value ere slowly increased. Microbial parameters, in product, (in zero time) were log 3 and 4 but after 30 days were log 4 and 5. Organoleptic factors such as odor, frangibility, taste in fish cheese in zero and 30 days were good and medium respectively. Cost of fish cheese was varied between 2000 to 27000 Rails. As fish cheese is prepared from sarcoplasmic proteins and was not any use from fish tissue, therefore it can be produced as secondary products in processing plants and can be introduced to people as new fish product.
    Keywords: Fisheries ; Iran ; Fish cheese ; Sarcoplasmic protein ; Silver carp ; Acid coagulation ; Hypophthalmichthys molitrix
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 66
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  • 9
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    Community diversity and abundance of ammonia‐oxidizing archaea and bacteria in shrimp pond sediment at different culture stages (2020)
    Wiley
    Details
    Publication Date: 2020-10-20
    Print ISSN: 1364-5072
    Electronic ISSN: 1365-2672
    Topics: Biology
    Published by Wiley
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  • 10
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    Improvement of gibberellin production by a newly isolated Fusarium fujikuroi mutant (2020)
    Wiley
    Details
    Publication Date: 2020-07-08
    Print ISSN: 1364-5072
    Electronic ISSN: 1365-2672
    Topics: Biology
    Published by Wiley
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