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  • Articles  (271)
  • Solanum tuberosum L.  (270)
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  • Chemical Engineering
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (271)
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  • Articles  (271)
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  • 1
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; potato tuberization ; growth regulators
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A reverse of the delaying effect of gibberellic acid (GA3) was observed in in vitro tuberization of potato plantlets when the medium contained 0.8×10−5 M jasmonic acid (JA). The promoting effect of JA seemed to be correlated with the initial absence of growing roots, probably through direct activity of JA in microtuber-producing buds in the stoloniferous shoots. This is based on the fact that JA did not inhibit root growth a posteriori. Endogenous gibberellins (GAs) synthesized by roots did not interfere with the previous activity of JA in tuberization. The absence of a JA promoting-effect on tuberization of previously-rooted plantlets could be related to the capacity of potato roots to synthesize endogenous GAs which might antagonize the JA effect on buds of stoloniferous shoots.
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  • 2
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    Potato research 43 (2000), S. 97-106 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; National List ; Recommended Trials ; growing crop ; tuber ; yield ; quality ; disease ; nematodes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In 1994, a postal questionnaire was sent to 41 institutes in 27 countries to obtain information on the national potato cultivar testing programmes in Europe. Responses were received from 19 countries. Foliage, tuber and yield characteristics were assessed in all countries but the number of sites and replicated varied considerably between the countries. Cooking and taste qualities were measured in most countries using relatively simple methods but processing characteristics were tested less commonly. Susceptibility to the most common diseases, i.e. late blight, common scab, leafroll and PVYo, was assessed in over 75% of the countries while resistance to another 21 disease and 4 potato cyst nematode pathotypes was assessed by various countries, depending on their national importance. Many of the assessments were based on records of natural infection although controlled inoculation tests were done more frequently for the more important diseases.
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  • 3
    ISSN: 1871-4528
    Keywords: actinomycetes ; resistance ; scab incidence ; Solanum tuberosum L. ; Streptomyces scabies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Concentrations of Ca, P, K, Mg, Al, Fe, Mn, Cu and Zn were determined in healthy tuber peelings of cultivars less (Karin, Santé and Symfonia) and more (Agria, Désirée and Tomensa) susceptible to common scab when grown at two sites that differed in the level of scab incidence. The accumulation of some elements was significantly influenced by site, year, cultivar, maturity and the age of tuber periderm. At both sites, Ca and P in periderm tissue declined but Mg increased during the growing season. The Ca/P ratios in tuber periderm of all cultivars greatly decreased 83 days after planting. Concentrations of mineral elements measured at harvest may not reflect conditions present during the infection period, and consequently may not be related to scab incidence or severity.
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  • 4
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; Storite Clear ; Storite Flowable ; radionuclide ; scintillation counting ; sprout suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The use of imazethapyr as a sprout suppressant in potatoes has been investigated. Novel radionuclide techniques were developed to establish the patterns of uptake and movement of imazethapyr in potatoes. Protocols for tissue analysis were established to homogenise potato tuber tissue samples, enabling radiolabel recovery by liquid scintillation counting to approach 100%. The movement of imazethapyr was also examined in the presence of an acidic formulation of thiabendazole which caused increased uptake. The movement of imazethapyr within the tuber was also influenced by pH and ion trapping.
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  • 5
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; damage ; bruising ; impact ; blackspot ; handling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the chain of handling operations from harvesting to packaging inclusive, potatoes are exposed to many brief mechanical forces (impacts) and to long-term pressure. Impact and pressure can lead to subcutaneous tissue discoloration causing qualitative and quantitative losses. We investigated the characteristics of construction and utilization of representative Dutch ware potato handling chains and found that the number and height of drops appeared to be too high, the use of cushioning materials insufficient and the speed of the transporting parts too high and not in balance with each other. The contribution to the total amount of subcutaneous tissue discoloration was 16% for the harvesting to bin filling phases inclusive, 22% for the storage phase, 27% for the shovelling to truck loading phases inclusive and 35% for the truck unloading to packaging phases inclusive. These contributions varied considerably within phases and within links. The results can be used to develop equipment, upgrade present chains and design new, produce-frendly handling chains.
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  • 6
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; damage ; bruising ; blackspot ; handling ; velocity change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potatoes are exposed to mechanical forces which can lead to subcutaneous tissue discoloration resulting in losses. We investigated the number and intensity of impacts in different phases of representative Dutch ware potato handling chains using an instrumented sphere (IS). Other questions we asked were, to which drop height onto a defined surface do measured impacts correspond, and what is the relation between the number of impacts and damage? Close relationships between peak acceleration and velocity change for different drop heights were found for various cushioning materials, thus allowing interpretation of impact measurements in terms of drops. The IS recorded c. 340 impacts exceeding 30g. The contribution to the total number of impacts was 11% for Harvest, 0% for Storage, 10% for Transport and 79% for Packaging. The number and intensity of impacts correlated well with the amount of subcutaneous tissue discoloration. It is concluded that the produce-friendliness of the handling chains can be improved.
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  • 7
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    Potato research 43 (2000), S. 253-261 
    ISSN: 1871-4528
    Keywords: laboratory tests ; storage ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary After a wet autumn and harvesting under wet conditions, slugs brought into potato stores via clods and soil adhering to tubers may cause substantial damage by feeding on the tubers. A carvone sprout inhibitor, Talent®, applied in the potato stores at a dose of 50 ml per tonne potatoes, controlled slugs within a few days. A dose of 25 ml carvone was insufficient. Using a laboratory box test, the slug (Deroceras reticulatum) affected only wounded tubers and, although slime was present on the unwounded potatoes, they were not damaged by slugs.
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  • 8
    ISSN: 1871-4528
    Keywords: Helminthosporium solani ; storage conditions ; disease resistance ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Optimum storage conditions to identify resistance to silver scurf among commercially grown potato cultivars were determined in a series of experiments. Inoculation of field-grown tubers with a conidial suspension ofH. solani (concentration 104 conidia per ml of water) and incubation at 15°C with 95% RH for 1 month followed by 2 months at 85% RH produced the greatest differences in disease severity between potato cultivars. Results with glasshouse-grown tubers were comparable with those from field-grown tubers, indicating that the test could be used to identify resistance in wild species of potato and to understand the inheritance of this resistance.
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  • 9
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    Potato research 43 (2000), S. 273-278 
    ISSN: 1871-4528
    Keywords: cold storage ; store management ; potato handling ; tuber temperature ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of using radiant heat to quickly warm potatoes and reduce damage in handling after cold storage was investigated. Two treatments were investigated: tubers dropped 350 mm with no radiant heat applied, and tubers dropped with radiant heat applied. Both treatments were compared with a control set of tubers which were taken directly out of store. The mean damage index per tuber was significantly reduced following radiant heat treatment although there were cultivar and size variations. The temperature profile under radiant heat was also measured which showed an increase in the outside flesh temperature of 8–12°C depending on tuber colour.
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  • 10
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    Potato research 43 (2000), S. 287-295 
    ISSN: 1871-4528
    Keywords: nutrient analysis ; culture media ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Boron (B) requirements differ widely among plant species and the concentration range between toxicity and deficiency is less for B than for any other nutrient. Excess B can adversely affect calcium (Ca) uptake and plant growth. Potato cvs Bintje and Norland plantlets were micropropagated on Murashige & Skoog (MS) nutrient medium, supplemented with 3 (MS control level) or 9 mM Ca, and a range of H3BO3 levels (0.025 to 0.300 mM B). Medium B levels of 0.100 and 0.300 mM decreased Ca content in leaves and shoots of cv. Norland, but not Bintje. Medium B level of 0.025 mM, which is 25% of the control MS level, enhanced Ca uptake in cv. Norland and did not compromise normal plantlet growth in either cultivar. This lower H3BO3 level (0.025 mM B), and a B-free gelling agent such as Gelrite, could be used for micropropagation of potato and possibly other species susceptible to Ca defifiency disorders.
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  • 11
    ISSN: 1871-4528
    Keywords: chemical inducible gene expression ; glutathione S-transferase ; Solanum tuberosum L. ; herbicide safener ; tuber
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Chemically inducible gene regulation systems provide a mechanism for a temporal control of expression of transgenes. In this study expression from the herbicide safener inducible maize GST-27 promoter was tested in potato, with the aim of using this to provide inducible expression of transgenes implicated in dormancy control. A binary vector comprising 3.8 kb of the GST-27 promoter was fused to the β-glucuronidase (GUS) reporter gene and transformed into potato. Application of the chemical inducer elevated the expression of GUS up to 40 fold in leaf tissue. However in stems, roots and tubers the GST-27 promoter caused high levels of expression of GUS in the absence of safener, demonstrating that in these tissues it acts as a constitutive promoter. A deleted promoter region of the GST-27 promoter displayed a similar expression pattern. Analysis of GUS activity in dormant and sprouting tubers showed that the GST-27 promoter was a strong constitutive promoter throughout the tuber life cycle.
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  • 12
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; plant development ; antioxidant genes ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The expression of antioxidant genes has been analyzed in a potato plant and during tuber dormancy. Manganese superoxide dismutase (MnSOD), cytosolic copper and zinc superoide dismutase (Cu/ZnSOD), catalase class II, cytosolic ascorbate peroxidase (APX) and glutathione peroxidase (GPX) are expressed at the RNA level in all the contexts analyzed. By contrast, the expression of the iron superoxide dismutase (FeSOD) and plastidic Cu/ZnSOD seems to be limited to green tissues, as shown by northern blots and native gels. A complex DAB-peroxidase isozyme pattern (using diaminobenzidine as substrate) has been observed in different developmental contexts analyzed, but hardly observed in tubers. During tuber dormancy, MnSOD and cytosolic Cu/ZnSOD activity was relatively constant in both Désirée and Bintje varieties while catalase activity decreases. Moreover, tuber dormancy breakage did not involve significant changes in the activity of these enzymes. On the basis of these results, the possible link between active oxygen species (AOS) metabolism and dormancy is discussed.
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  • 13
    ISSN: 1871-4528
    Keywords: root-water uptake ; modeling ; SWACROP ; Ferralsols ; tropics ; Cuba ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The SWACROP root-water uptake function for potatoes was determined in the 1994–95 crop season on a Ferralsol in Havana province, west Cuba. The maximum pressure-heads under which water is optimally extracted by potato roots were very similar to the original Feddes function. Nevertheless, the anaerobiosis point and the wilting point pressure-head in both functions were not in agreement. However, the simulated values of soil water contents in the 0–40 cm soil depth by performing SWACROP runs in the 1995–96 potato crop season using both functions were very similar. The original Feddes function seems to be good enough for predicting soil water contents in the tropics and no specific other functions need to be developed for any particular soil or climate condition.
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  • 14
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    Potato research 43 (2000), S. 43-48 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; Decis® ; Colorado potato beetle ; tuber quality ; dry matter content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Tubers showed changes in their nutritional value caused by the treatment of the plants with the insecticide deltamethrin. The level of starch was increased in comparison with control tubers; but the amylose percentage was not affected. In contrast, the total protein content decreased in tubers from treated plants, whereas free amino acids level and ascorbic acid content increased. The results showed that the changes in the tubers caused by the treatment of potato plants with deltamethrin did not adversely affect the nutritional value of the progeny tubers.
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  • 15
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    Potato research 43 (2000), S. 49-59 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; soil fertility ; soluble salts ; plant spacing ; Rhizoctonia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Excessive fertilizer application in North Carolina, USA results in high residual soil levels of P, K and total soluble salts in potato fields. Our objectives were to determine the salt sensitivity of three common cultivars (Atlantic. Snowden and Superior), and the effects of planting density and fertilization on stand establishment, soilborne disease, and yield. Greenhouse results suggest that potato growth and emergence, but not shoot initation, are reduced by high salinity. On a Portsmouth fine sandy loam, high fertilizer rates did not reduce stands of cv. Atlantic in the field in 1996 or 1997. Excess N resulted in moreRhizoctonia stem canker in 1996, and lower yields at the lower planting density in 1997. Yield compensation for reduced plant density occurred in 1997 at the lower N rates (56 and 112 kg N ha−1) and at the high PK rate (49 kg P and 139 kg K ha−1).
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  • 16
    ISSN: 1871-4528
    Keywords: transgenic plants ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Helicoverpa armigera is one of the important insect pests adversely affecting the yield of potatoes in India. A synthetic gene encoding the insecticidal crystal protein (Cry1Ab) ofBacillus thuringiensis (Bt) has been introduced into five genotypes of potato usingAgrobacterium tumefaciens. Southern analysis of DNA from transgenic plants confirmed the integration and copy number of the transgene. Double-antibody quantitative sandwich ELISA analysis demonstrated high levels of Cry1Ab protein expression in transgenic plants. Insect bioassays on the leaves of transgenic plants showed considerable protection against the larvae ofH. armigera in terms of leaf area consumed and larval weight reduction.
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  • 17
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; sexual reproduction ; oospores
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The severity of late blight tuber rot in five potato cultivars (Green Mountain, Sebago, Dorita, AC Brador and Island Sunshine) resulting from inoculation with US-1/US-8 combinations of Canadian isolates ofPhytophthora infestans was evaluated. In addition, the potential for mixed (A1∶A2) inoculations to result in oospore formation was examined. In studies conducted in 1996 and 1997, cv. Dorita (and Island Sunshine in 1997) developed the least amount of surface or internal necrosis relative to the other cultivars following co-inoculation with US-1 and US-8 isolates ofP. infestans. Differences also were revealed among isolate combinations used for inoculation. The combination of a US-1 isolate from Prince Edward Island with a US-8 isolate from New Brunswick consistently produced the least amount of necrosis in tubers of the various cultivars. There was no evidence of oospore production in any of the tubers examined.
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  • 18
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; prickle pollination ; ploidy level ; flow-cytometry ; adventitious shoots ; in vitro tuber induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary For the induction and selection of double mutants with altered starch composition, a uniform (homohistont) amylose-free (amf) monoploid as basic plant is of vital importance. Therefore, newamf monoploids had to be developed via prickle pollination. And 26 monoploids were obtained which were screened in vitro for vigour, leaf size and for the percentage of monoploid cells. The number of monoploid cells was underestimated at about 28%. Seven vigorous monoploids were tested in vitro for tuberization capacity, which varied from 0 to 100%. After regeneration in vitro, 0 to 3.7 adventitious shoots per leaf explant were formed. The tuberization capacity of these shoots varied from 0 to 48. Twoamf monoploids were selected which were vigorous and tuberized well in vitro. They are the basis for the application of an in vitro mutation breeding protocol that should lead to the induction and selection of new starch mutants in potato.
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  • 19
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    Potato research 42 (1999), S. 215-221 
    ISSN: 1871-4528
    Keywords: potato virus Y ; strain differentiation ; RFLP ; potato tuber necrotic ringspot disease ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A procedure for differentiating PVYNTN from PVYN is described and is based on the unique cleavage of their respective PCR products with strain specific restriction endonucleases. The PCR products corresponding to the 5′ end of the N and NTN strains of PVY were cloned and sequenced, and a restriction map was constructed which included common enzymes that were used for the differentiation of PVYNTN. Unique, single cleavage of PCR products derived from the 5′ end of the PVYNTN genome by Nco I, and that of the N-strain of PVY by Bgl II restriction endonuclease were demonstrated. The specific digestion patterns in polyacrylamide gel were used for the unequivocal differentiation between the N and NTN strains of the virus. Both single and mixed infections were detected in field samples of potatoes using this procedure.
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  • 20
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    Potato research 42 (1999), S. 229-240 
    ISSN: 1871-4528
    Keywords: earliness ; planting season ; Mediterranean area ; off-season production ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In Italy, the early potato cultivars of greatest economic significance are those grown off-season, in a cycle which is generally much earlier than the typical spring-summer cycle. Of the area given over to early potatoes in Italy, more than 90% is in the southern regions. The economic value of this production is due to its extraseasonality, which allows much of the early potato harvest to be sold on foreign markets. In Sicily, more than 5000 ha of potato are grown, with a high frequency of potato crops on small plots. Early potatoes are often rotated with various fruits and vegetables, and the length of the cycle is variable, from 100 to 140 days. Choosing the most suitable cultivar is the most complex aspect of early potato growing. Of the criteria used, tuber growth rate has shown most validity. In Sicily two cultivars are mainly grown: Sieglinde and the more recent but well-established Spunta. Agricultural techniques used for early potato cultivation vary depending on the area. An overview of the agronomic practices used in different areas is given in this paper.
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  • 21
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    Potato research 42 (1999), S. 95-99 
    ISSN: 1871-4528
    Keywords: analysis ; HPLC ; filter paper model ; Diels-Alder reaction ; 1,2-dihydro-3,6-pyridazinedione ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method used for estimating extractable maleic hydrazide (MH) concentrations in fresh potato material, concentration range 5–17 mg kg−1, was found not to be suitable for processed potato products (10–33% recoveries) although, boiling potato pieces enhanced recovery by 20%. Each step of the determination was examined and a modified procedure developed with particular emphasis on the extraction of MH from the dried potato matrix, and the quality of the HPLC column used. Potato slices and model systems based on filter papers plus additives were used. Recoveries from fried potato slices were 74±6%. Based on the effect of glucose in reducing extractable MH recoveries, it is suggested that the remainder of the MH (20–25%) is converted into a conjugated structure on reaction with dehydrated sugar (Diels-Alder reaction).
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  • 22
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    Potato research 42 (1999), S. 89-93 
    ISSN: 1871-4528
    Keywords: analysis ; HPLC ; β-glucoside ; 1,2-dihydro-3,6-pyridazinedione ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The method of Vadukul (1991) for determining maleic hydrazide (MH) was modified and gave recoveries of free MH of 89%±4%. The values recorded on individual tubers ranged from 2–14 mg kg−1. Maleic hydrazide was evenly distributed throughout the tuber (peel, outer and inner flesh) but concentration increased slightly as tuber size increased. The concentration of free MH decreased from 7 to 3 mg kg−1 over the storage period of 5 1/2 months. Acid hydrolysis released substantial amounts of MH particularly from older potatoes (13 mg kg−1) compared with 6 mg kg−1 from new potatoes, implying that free MH is gradually converted to a bound form with time after treatment. No evidence was found for the presence of a β-glucoside of MH.
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  • 23
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    Potato research 42 (1999), S. 585-591 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; microtubers ; acetic acid ; propionic acid ; asscorbic acid ; salicylic acid ; acetylsalicylic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato microtubers are usually induced in media containing growth regulatory, typically cytokinins and growth retardants. However, since these substances may imbalance the physiology of the cultured explants and cause adverse carry-over effects on the subsequent performance of the microtubers, the potential acid-induction of in vitro-mass tuberization was investigated as an alternative. For this purpose, a range of explant types (stolons, single-node or apex-containing multinodal sections), organic acids (acetic, propionic, ascorbic, acetylsalicylic or salicylic acid), and photoperiods (continuous dark, 8 or 16 h photoperiod) were established. Gellified medium or raft membranes on liquid medium were also tested. Although variations due to explant type, photoperiod, organic acid supplemented, and medium type were found, all organic acids tested under the different experiments caused tuberization. Multiple tuber formation or bigger tubers did not develop on multinodal explants. In terms of tuberization rate and mean tuber weight, gellified medium performed better than liquid medium with rafts. The recently established role of the salicylic and acetylsalicylic acids on tuberization is corroborated by our system. The results with the acetic, propionic and ascorbic acids show that in vitro hormone-free tuberization can be easily and rapidly achieved.
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  • 24
    ISSN: 1871-4528
    Keywords: growth regulator ; sucrose ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ancymidol-induced in vitro tuberisation under different photoperiodic regimes (dark, SD=8 h, LD=16 h) and sucrose concentrations (2, 4, 6 and 8%) in cv. Jaerla (early) and cv. Baraka (late) was studied. Tuberisation was scored after 4 (dark) or 8 (light) weeks. Ancymidol significantly increased tuberisation under LD-SD for any sucrose concentration in both cultivars. Ancymidol also significantly increased tuberisation under LD-dark for 6% sucrose in both cultivars. Tuberisation in cv. Jaerla was significantly higher than in cv. Baraka for any photoperiodic treatment in the presence or absence of ancymidol with 4, 6 or 8% sucrose. The photoperiod SD-SD resulted in the higher level of tuberisation for any combination of sucrose, cultivar and ancymidol. The possible role of ancymidol is discussed.
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  • 25
    ISSN: 1871-4528
    Keywords: cultivar ; virus diseases ; nematodes ; ground cover ; physiological age ; seed production ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In Argentina, different areas are suitable for seed potato production: however, each has particular problems, mainly related to different pest and diseases. Tierra del Fuego Island is well isolated from traditional potato growing areas. Therefore, it was tested for its potential for seed production. The cultivars Achatt. Mailén INTA. Pampeana INTA and Spunta were grown in Río Grande, San Pablo and Ushuaia from 1991–1994. Nematode presence and aphid population dynamics were recorded. Average tuber yield ranged from 20.1–37.6 t ha−1. After three years PVY and PLRV remained low (0–1% for different cultivars and locations). Moreover, seed tubers obtained were physiologically young. Long days, early frosts and strong winds may limit tuber yield in some years. The island can be considered as an ecological “safe haven” and is very suitable to obtain healthy and physiologically adequate seed potatoes.
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  • 26
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    Potato research 42 (1999), S. 25-29 
    ISSN: 1871-4528
    Keywords: chlorpropham ; particulates ; dusts ; solvents ; ignition source ; explosive limits ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An appraisal was undertaken of the key factors involved in explosion and combustion processes mainly associated with the fogging of chlorpropham formulations into potato stores. The key factors considered are source of ignition, solvents, explosive limits of solvents, the presence and atmospheric concentrations of particulate clouds and the role of particulate material in dust explosions. Also the sequence of events that takes place in a dust explosion, including critical concentrations of dust required to initiate the reactions involved and the particular relevance of the above events to the behaviour of chlorpropham fogs both at the time of application and when distributed throughout a store. The relevance of fine dust already present in the store to the initiation of explosions, particularly secondary explosions, which are considered to be a major cause of concern due to their severity, is also emphasised.
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  • 27
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; tuberisation ; extensin ; acyl carrier protein thioesterase ; high mobility group protein ; gene expression ; plant development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In screening to isolate a full-length copy of a previously isolated cDNA clone, a further three cDNAs were also isolated from a library prepared from sub-apical swelling-stolon tissue of potato (Solanum tuberosum L.). Sequence analysis showed these clones to be similar to extensin-like protein genes, acyl carrier protein thioesterase genes and high mobility group protein genes, respectively. A further cDNA, isolated by subtractive hybridisation, was similar to a tomato cDNA previously isolated on the basis of its down-regulation following nematode infection. While all the newly isolated genes were expressed in swelling stolons, for most, maximal expression was seen to be in stem tissue. Possible roles for these genes in the development of potato plants are discussed, as is the significance of gene expression in stems and stolons to the process of tuberisation.
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  • 28
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    Potato research 42 (1999), S. 241-264 
    ISSN: 1871-4528
    Keywords: global distribution ; prospects ; diversification ; specialization ; price risk ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We examine the changing character of supply and demand for potatoes over time and space with an eye towards implications for agricultural research. Diversification in consumption and specialization in production are our organizing themes. The foundation for comparative analysis is a map of the global distribution of potato growing area. We highlight the adverse impact of price risk on specialization and discuss several sources of uncertainty that condition the size but not the direction of major trends.
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  • 29
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    Potato research 42 (1999), S. 279-282 
    ISSN: 1871-4528
    Keywords: late blight ; mating types ; sexual recombination ; Solanum tuberosum L. ; diversification ; aggressiveness ; migration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Since the early 1980s and continuing through the end of the 20th century, potato late blight caused byPhytophthora infestans has become noticeably more problematic throughout the world. Exotic strains of the pathogen are largely responsible for the increased problems. The new strains were initially detected in Europe, but subsequently they have been detected in Asia, the Middle East, South America and North America. Populations in the Middle East and South America may represent secondary migrations from Europe. Several independent migrations have occurred such that migrant populations in North America are very different from those in Europe. Also, population structures ofP. infestans are different in different worldwide locations. In some locations the migrant strains are resistant to metalaxyl, but in other locations either migrant or indigenous strains may be resistant. In most locations, the migrant strains are more aggressive than the previous indigenous strains.
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  • 30
    ISSN: 1871-4528
    Keywords: wild species ; gene introgression ; heat stress ; drought stress ; frost stress ; biotic stresses ; off-season production ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the Mediterranean area the potato is a very important crop and can be cultivated throughout the year. However, tuber yields are generally much lower than those in Northern European countries. This is because the environmental and agro-economic conditions are different from those of Northern countries where most cultivars were developed. We feel that the objective for the “new Mediterranean potato” must be the availability of potato cultivars for each environment and for each planting season. Breeding programmes should focus on improving potato production within a specific agro-ecological Mediterranean area rather than improving crop production across areas. This paper reports aspects of potato breeding and physiology related to resistance to stress conditions in the Mediterranean area, with particular emphasis given to resistance to abiotic (heat, drought and frost) as well as biotic stresses. We also discuss the potential to produce seed tubers suitable for potato cultivation in the Mediterranean area.
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  • 31
    ISSN: 1871-4528
    Keywords: genes ; transgenic plants ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Continuing study of the potato plant's response to stressful conditions has led to the identification of a large number of plant genes whose expression, is regulated by external stimuli. Stress-induced genes can be broadly divided into functional or regulatory genes. To the first category belong genes encoding proteins or enzymes of plant metabolic pathway, of molecules involved in repairing cellular damages and/or indispensable for restoring a new cellular homeostasis compatible with the external conditions. The other class includes genes primarily involved in the perception and/or intracellular transduction of the stress signal, such as kinases, phosphatases or transcription, factors. The research objectives in the field of plant stress tolerance has recently evolved from a mere cloning and description of stress-induced genes to the design of the best strategy of producing transgenic plants tolerant to environmental constraints. It is well known that stress tolerance is a complex trait, requiring the coordinated regulation of a network of genes that act synergistically and additively. At best, manipulation of one single down-stream gene may contribute only partially to the tolerance of the transgenic plants. Recent studies have shown that it is feasible to regulate the level of expression of many down-stream stress-induced genes in a coordinated fashion by regulating the expression of genes encoding transcription factors able to bind DNA motifs in the promoter of stress-induced genes. However, the constitutive high level of expression of transcription factors often causes detrimental phenotypic effects. This drawback could be bypassed by putting genes for transcription factors under the control of inducible promoters. In this way, endogenous tolerance genes are activated only when the stress event occurs, minimizing the negative pleiotropic effect. Novel technology (reverse genetics, DNA microarrays, mRNA differential display, T-DNA tagging, complementation and over-expression of plant cDNA in yeast as model for cellular stress tolerance), improvement of genetic transformation techniques (multiple gene transfer, gene targeting by homologous recombination) as well as a better efficiency of foreign gene expression (discovery of plant promoters with cell-specific, tissue-specific, developmental stage-specific, and/or inducible patterns of expression) will give a tremendous impulse to produce stress tolerant commercial cultivars of the main crops through genetic engineering.
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  • 32
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    Potato research 42 (1999), S. 381-395 
    ISSN: 1871-4528
    Keywords: agronomy ; breeding ; pathology ; physiology ; Solanum tuberosum L. ; storage ; utilisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This contribution presents highlights of the oral and poster presentations in the various sessions of the 14th Triennial Conference of the European Association for Potato Research, held in Sorrento, Italy, May 1999. It is based on the reports of the session chairpersons and the book of abstracts. The paper reports the progress made in many aspects of potato research, but also stresses the need for further collaborative work, especially in the fields of molecular physiology, marker assisted breeding, control of late blight, potato tuber moth and other pests and diseases, stress physiology and stress resistance, and sustainable cropping systems with both short and long cycle potato crops.
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  • 33
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    Potato research 42 (1999), S. 489-498 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; microtubers ; nodal cuttings ; in vitro tuberization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A tissue culture technique is described in which nodal cuttings ofSolanum tuberosum L. are induced to form usable microtubers in order to produce high health status of the seed potato genotypes cultivated in Switzerland.
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  • 34
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    Potato research 42 (1999), S. 499-504 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; microtuber ; liquid medium ; temporary immersion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary CIRAD developed a new apparatus for plant tissue culture, using temporary immersion in a liquid medium. This apparatus was adapted to the microtuber production in potato. The procedure is as follows: single node cultivation on MS medium containing 30 g/l sucrose in the light for 2 weeks, induction of microtuberisation with 80 g/l sucrose over a 2 week period in the light, followed by a further 6 weeks in the dark. All experiments were performed at 20 °C. The basic vessel had a capacity of approximately 11;30 nodes were cultivated per vessel. Depending on the cultivars tested (Bintje, Ostara and Désirée) 47 to 115 microtubers were harvested per vessel. Between 30 and 60% of the microtubers weighted over 0.5 g and between 10 and 40% over 0.8 g. Sprouting is still under investigation. Preliminary results indicate that the dormancy period was relatively short and several stems were obtained per microtuber. These results seem to be better than those usually reported. Only one simple protocol has been tested and further improvements are probably easy to obtain.
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  • 35
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    Potato research 42 (1999), S. 607-610 
    ISSN: 1871-4528
    Keywords: seed production ; Greece ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Large scale, commercial production of potato minitubers, cvs Spunta, Jaerla and Kennebec, has been carried out in VITRO HELLAS S.A. since 1995, according to protocols based on in vitro methods. Apical meristems were cultured on MS medium supplemented with 1 μM IBA, 20 g/l sucrose and 6 g/l agar (pH 5.8). Subculture was carried out every 15 days. Multiplication rate was 4–5 per cycle. Self-rooted microplants were transferred into screen houses (200 plants/m2) in a mixture of peat and perlite 1∶1 (v/v). Cultivation period was 15 August to 15 November each year. During the period 1995–1997, the number of minitubers produced/plant was 2.07 for cv. Spunta, 1.85 for cv. Jaerla and 2.52 for cv. Kennebec and their average weights were 10.8 g, 10.9 g and 9.8 g, respectively. The percentage of minitubers〈10 mm in diameter was 2%, 50.1% were 10–20 mm and 47.9% were〉20 mm.
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  • 36
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    Potato research 42 (1999), S. 611-617 
    ISSN: 1871-4528
    Keywords: dormancy ; potato ; losses ; Solanum tuberosum L. ; seed potato production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Microtubers can be stored longer, transported and handled easier than plantlets, but they have some disadvantages related to long dormancy. Dormancy, number of sprouts per tuber and loss of microtubers under different storage temperatures were studied. Microtubers of four genotypes originated from different tuberization treatments (photoperiod combinations) were observed. We found that dormancy depended on cultivar and — in some cases — on the photoperiod treatment applied during tuberization. Generally, the dormacy was long and was greatly elongated by low storage temperature. One of the photoperiod treatments shortened while the other treatment prolonged the rest-period compared to the control, maybe due to a change in temperature accompanying the change in daily light (photoperiod combination). Besides, these treatments affected the number of sprouts per tuber. There was no significant difference in duration of dormancy between different tuber-size groups, but the loss increased significanctly with a decrease in tuber size. Based on this information we can use microtubers more effectively in the seed potato production programme.
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  • 37
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    Potato research 42 (1999), S. 73-78 
    ISSN: 1871-4528
    Keywords: growth parameters ; K+/Na+ ratio ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Increased potassium (K) fertilization of saline soils has been implicated in alleviating salt stress in plants. We examined whether varying K concentrations in Murashige & Skoog (MS: 1962) basal medium could affect salt (NaCl) stress in micropropagated potatoes. Plantlets of cvs Sierra and Russet Burbank were evaluated after 1 month of growth in a medium containing 0. 40, or 80 mM NaCl and 6,20, or 30 mMK. The medium K was adjusted using KNO3 while total nitrogen was kept constant using NH4NO3. Growth parameters were less affected in Sierra than Russet Burbank, and roots were less affected than shoots, as the medium salinity increased. Tissue Na levels were greater when the medium K was 6 mM compared with 20 mM MS control. The medium K concentration 50% greater than MS control did not promote growth and did not limit tissue Na levels. While this should be confirmed by field assessment, our results suggest that while K deficiency promotes salt damage, its addition beyond the usual fertilizer recommendations is not beneficial in alleviating salinity stress.
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  • 38
    ISSN: 1871-4528
    Keywords: Solanin ; Chaconin ; Magnesium ; Kalium ; Stickstoff ; Solanum tuberosum L. ; Phytophthora infestans ; Feldversuch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary Field experiments were carried out for three years to study the effect of various magnesium, potassium and nitrogen fertilization rates on two medium-early edible potato cultivars. Additionally, one of the experimental areas was sprayed againstPhytophthora infestans to ascertain the effect of fungicides on the content of total glycoalkaloids (TGA) in potato tubers after harvest and after six months of storage. The applied fungicide treatments increased total glycoalkaloids (TGA) of potato tubers (Fig. 4). Fertilization with mineral nitrogen caused different reactions in both cultivars. There was a tendency for cv. Beryl to accumulate more glycoalkaloids with increasing nitrogen fertilization rates, while the opposite was observed for cv. Mila (Fig. 7). Fertilization with potassium and magnesium did not affect the results. On the basis of these results there was no conclusive evidence that the TGA content in the tubers was dependent on cultivar or on the weather during the growing season (Figs 1,2 and 3). The TGA content increased during storage, independently of previous treatments, (Figs 4–7).
    Notes: Zusammenfassung Es wurden Feldversuche in drei Jahren mit zwei Speisekartoffelsorten, verschiedenen Mengen an Mineraldüngern und der Anwendungen eines Pflanzenschutzmittelns gegenPhytophthora infestans angelegt. Die Knollen wurden direkt nach der Ernte sowie nach einer sechsmonatigen Lagerung auf Glykoalkaloidgehalt untersucht. Zwischen den Sorten treten statistisch gesicherte Unterschiede im Gehalt an Glykoalkaloiden auf. Die Anwendung von Pflanzenschutzmitteln während der Vegetation erhöht den Gesamtgehalt an Glykoalkaloiden (TGA) in den Kartoffelknollen kurz nach der Ernte. Eine variierte Mineraldüngung hat keinen statistisch signifikanten Einfluss auf den TGA-Gehalt in den Proben kurz nach der Ernte, N- und K-Düngung verändern diesen jedoch im Laufe der Lagerung. Unabhängig von den übrigen Bedingungen erhöht sich der TGA-Gehalt in diesem Versuch während der Lagerung im Mittel von 5,8 auf 7,7 mg/100g Frischsubstanz.
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  • 39
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    Potato research 42 (1999), S. 101-105 
    ISSN: 1871-4528
    Keywords: late blight ; testing methods ; electrolyte leakage ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The resistance of ten potato cultivars (Agria, Ajax, Désirée, Liseta, Kennebec, Majestic. Monalisa, Prima, Spunta and Tonda di Berlino) toPhytophthora infestans was analyzed in vitro using 8 fungal strains. An assay based on electrolyte leakage was used for screening leaves and tuber tissues with fungal culture filtrates. With almost all cultivars the resistance of leaves did not correlate with the resistance of tubers. Cv. Ajax appeared the least susceptible in both leaf and tuber tests, while the cv. Prima was the most susceptible in tuber tests.
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  • 40
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    Euphytica 102 (1998), S. 275-280 
    ISSN: 1573-5060
    Keywords: Germplasm conservation ; in vitro conservation ; minimal growth ; slow growth ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Combined effects of sucrose, mannitol and photoperiod on microplant conservation were studied in four potato genotypes belonging to two different groups viz., Tuberosum and Andigena. Minimal growth medium was based on Murashige and Skoog (MS) supplemented with 6 different concentrations of sucrose (30, 40, 50, 60, 70 and 80 gl-1) with 4 different concentrations of mannitol (0, 20, 40 and 60 gl-1). The cultures were conserved under two photoperiod conditions i.e. continuous illumination and 16-h photoperiod at 6 ± 1 °C. There were significant interactions between photoperiod and sucrose, and between photoperiod and mannitol. Maximum microplant survival and desirable microplant growth were observed under 16-h photoperiod. Sucrose alone did not improve culture viability over 30 months of storage. Inclusion of mannitol in the conservation medium increased microplant survival. Sucrose x mannitol interaction showed that sucrose was effective in enhancing microplant survival in combination with 20 or 40 gl-1 mannitol, but not with 60 gl-1 mannitol. Combined effect of sucrose, mannitol and photoperiod showed that optimum microplant growth and maximum culture viability were obtained when the cultures were grown in MS medium containing 40 gl-1 sucrose and 20 gl-1 mannitol under 16-h photoperiod. Potato microplants can be conserved in this medium and cultural conditions up to 30 months without subculturing.
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  • 41
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; Phytophthora infestans ; inducer agents ; plant protection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Tests for systemic induced resistance (SIR) to late blight were performed with different potato cultivars in field trials and in growth chamber experiments. The non-pathogenPhytophthora cryptogea (Pc) and salicylic acid (Sa) were used as inducer agents. In the field, plants were naturally infected byPhytophthora infestans while the indoor plants were inoculated. The degree of SIR obtained varied with the cultivar used and also with the type of inducer. For example in the field the susceptible cv. St. Cecilia had enhanced resistance with Pc whereas Sa caused increased susceptibility. Inbred resistant cvs acquired increased resistance after treatment with Sa. In a separate pilot experiment in a growth chamber, Sa at two different concentrations was injected into potato seed tubers before planting. The higher Sa concentration increased resistance in genetically resistant cultivars while the lower Sa concentration brought about increased susceptibility.
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  • 42
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    Potato research 41 (1998), S. 117-125 
    ISSN: 1871-4528
    Keywords: α-solanine ; α-chaconine ; C:S ratio ; glycoalkaloid content ; tuber size ; individual tubers ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plants of early potato cultivars were sampled at successive harvests during tuber development and individual tubers were analysed for α-solanine and α-chaconine using high-performance liquid chromatography. Mean tuber total glycoalkaloid concentration (α-solanine plus α-chaconine) per plant decreased with time and statistically significant differences between cultivars were observed in the patterns of decline during tuber growth. The mean tuber ratio of α-chaconine to α-solanine increased during tuber development and was also affected by genotype. Total glycoalkaloid concentration for individual tubers of marketable sizes (up to 50 g fresh weight) exceeded the safety limit of 20 mg per 100 g fresh weight for cultivars Home Guard and British Queen but not for Rocket. These differences were attributable to differences in both rates and patterns of glycoalkaloid accumulation during tuber development, although in all cultivars results were consistent with glycoalkaloid accumulation occurring in the expanding peripheral cell layers of tubers for a considerable period after tuber initiation.
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  • 43
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    Potato research 41 (1998), S. 305-310 
    ISSN: 1871-4528
    Keywords: potato late blight ; mating types ; oospores ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In this paper we present observations made during three years in a potato field in Sweden that indicated the presence of soil borne inoculum ofPhytophthora infestans. In 1994, a fungicide trial against late blight was left in the field with various degrees of late blight infection. In 1995, cereals was grown in the field. In 1996, certified potato seed tubers were planted and blight appeared early in the season in a pattern corresponding to the heavily infected areas in 1994. No volunteer potato plants were found in 1995 or 1996. Both mating types A1 and A2 were isolated in 1996 and 1997 and oospores were found in leaves, stems as well as in stolons.
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  • 44
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    Potato research 41 (1998), S. 311-317 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; general combining ability ; specific combining ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In an epiphytotic year of early blight, data were recorded on its incidence in 72 progenies (18 females x 4 males) and their parents planted in a randomised complete block design at the Punjab Agricultural University, Ludhiana, India. Combining ability analysis showed that both additive and non-additive gene action were important, with a preponderance of the latter in the inheritance of resistance to early blight. In general, progenies were as good as or better than mid-parent values. Crosses involving both or at least one parent with good general combining ability resulted in heterotic early blight resistant progenies. Promising parents and crosses were identified.
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  • 45
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    Potato research 41 (1998), S. 345-354 
    ISSN: 1871-4528
    Keywords: tuber blight ; Solanum tuberosum L. ; disease assessment ; fungal pathogenicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Variation in aggressiveness to tubers among isolates ofPhytophthora infestans sampled from three potato growing regions in the Netherlands was compared. Variation in the ability to infect tubers of cv. Bintje was found between isolates of each of the three regional populations. The most aggressive isolate of the old population matched the average level of the new population in its ability to infect tubers. As a consequence, the commonly used reference isolate VK 6C can no longer be considered to be representative of the present population ofP. infestans. Therefore it is recommended that testing tuber resistance for the official list of potato cultivars with this isolate should be discontinued. Tuber infection and subsequent spread of the fungus in the tuber tissues were not found to be correlated. The components of tuber pathogenicity studied were not correlated to pathogenicity factors in the foliage, as measured under growth chamber conditions.
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  • 46
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    Potato research 41 (1998), S. 277-287 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; quality ; bruising ; external ; internal ; model ; index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Methods for quantifying external damage and bruising can be inaccurate and result in an index that is difficult to interpret. A new index was derived by taking measurements of peeled slices from cv. Record and developing a mathematical model to predict the percentage volume removed per peeler stroke for a range of potato cultivars. It was found that an average of 1.25% of the tuber volume was removed per peeler stroke. By counting the number of peeler strokes to remove damaged tissue and multiplying by 1.25, an estimate of the percentage tuber volume lost due to bruising or external damage can be obtained. The index can accommodate most types of damage and result in an index that is readily understood.
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  • 47
    ISSN: 1871-4528
    Keywords: post harvest disease ; potato ; Solanum tuberosum L. ; resistance ; toxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Virulence of 12Fusarium oxysporum isolates was determined on three potato cultivars Late Harvest, BP1 and Kimberley Choice. The production of fusaric acid by the 12F. oxysporum isolates was also determined. The aim of the study was to determine whether a correlation exists between fusaric acid production and virulence ofF. oxysporum isolates. Late Harvest was the most tolerant and BP1 the most susceptible cultivar. Virulence ranking, order of theF. oxysporum isolates with the different cultivars corresponded with the mean of value of the ranking order for all three cultivars. A correlation was found between virulence of isolates and fusaric acid production except for Late Harvest. Fusaric acid production may play a major role in the development of dry rot in potato tubers.
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  • 48
    ISSN: 1871-4528
    Keywords: potato blight ; hydrolytic enzymes ; chitinases ; glucanases ; phytoalexins ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The kinetics of accumulation of chitinases, glucanases and phytoalexins were studied in two potato cultivars, differing in their degrees of vertical and horizontal resistance, when infected with two races ofPhytophthora infestans. Tuber disks of cvs Kennebec (susceptible, low horizontal resistance) and Huinkul (tolerant, high horizontal resistance) were infected with either race “0” (avirulent) or “C” (complex, race 1.3.5.7.11) ofPhytophthora infestans. Extracts of tuber tissue (0–7 days) from cv. Kennebec infected with race “0” showed a strong increase in phytoalexin production and in chitinase and glucanase activities when compared with those infected with race “C”. These results indicate that race “C” is able to block defensive reactions. No significant differences were observed in cv. Huinkul infected with both races. The contribution of these reactions to horizontal resistance is unknown, and our results would not support a conclusive role for them in the interaction.
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  • 49
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    Potato research 41 (1998), S. 327-334 
    ISSN: 1871-4528
    Keywords: near infrared spectrometry ; modified partial least squares regression ; quality characterisation ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The development of a method of NIR (near infrared) spectrometric analysis to measure the quality determining constituents of potatoes and the accuracy of its performance are presented. The results show that it is possible to obtain quantitative information about different constituents from reflectance measurement of homogenized peeled potatoes. The analytical performance of NIR spectrometry is highly dependent on the cultivar being measured. Consequently, the calibration and validation sample sets will have to be expanded to provide greater utility.
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  • 50
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; drought tolerance ; irrigation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato crops in subtropical climates are often subjected to water stress, resulting in low yields and poor tuber quality. The yield response of potato genotypes to water supply was investigated in six trials, three each in spring and autumn plantings. Trials were conducted under automated rain shelters, using an irrigation boom to impose five soil water regimes. The negative effect of water stress on tuber yield was more detrimental in spring than in autumn, because of higher atmospheric evaporative demand and higher temperatures in spring. An average yield reduction of 68% was recorded for the most severely stressed regime in spring plantings, while the mean reduction for the same treatment in autumn was only 42%. Genotypic differences in yield response to drought were clearly illustrated in spring, but not in autumn. This suggests that the choice of genotypes is only influenced by the availability of water in spring, when more drought tolerant genotypes should be used.
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  • 51
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    Potato research 41 (1998), S. 1-8 
    ISSN: 1871-4528
    Keywords: immobilisation ; filter paper ; dot RT-PCR ; print RT-PCR ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A test procedure for PSTVd is described based on immobilisation of plant sap on filter paper, by dotting or tissue printing followed by RT-PCR. Tests were carried out using primarily and secondarily infected potato plants, primarily infected in vitro plants, and potato tubers. Print PCR was shown to be suitable for testing large samples of potato plants whereas dot PCR is recommended for in vitro plantlets and tuber tissue. Bulking one infected plant to 4 or 9 healthy plants gave reliable results with secondarily infected potato plants, but sometimes the test failed to detect PSTVd in primarily infected in vitro plants. Dotted and printed paper squares could be stored at 4°C for at least 2 weeks in Triton X-100 solution or under dry conditions. Storing at room temperature can lead to unreliable results.
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  • 52
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    Potato research 41 (1998), S. 21-28 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; general combining ability ; seedling generation ; clonal generations ; genotypexenvironment ; yield ; tuber colour and shape
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary General combining ability and its repeatability in the early generations of a potato breeding programme was studied by evaluating 72 progenies (18×4) of 22 parents in a factorial mating design for three successive generations. The mean squares for combining ability due to females, males and females × males were significant for eight out of the ten characters studied. Variance estimates due to specific combining ability were more important than those due to general combining ability for most of the characters in all the generations. General combining ability effects varied from generation to generation. Most of the correlation coefficients between generations for general combining ability effects were significant, but of moderate magnitude (0.5–0.8). Parents with consistent performance over generations and those specifically suitable for a TPS crop were identified.
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  • 53
    ISSN: 1871-4528
    Keywords: prochloraz ; Solanum tuberosum L. ; soil treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soil in a potato field naturally infested with black dot (Colletotrichum coccodes) was fumigated with methyl bromide at 126 g m−2 or left unfumigated. Potato seed tubers (cv. BP1) uninfected, lightly infected (1–25% surface affected), severely infected (26–100% surface affected) and severely infected withC. coccodes but dusted with prochloraz manganese chloride as Octave 2.5% DP at 750 g per 100 kg seed were planted in fumigated and unfumigated soil. When harvested, the incidence of black dot on the progeny of infected seed planted in unfumigated soil was twice that of progeny in fumigated soil, with progeny of uninfected seed having a 68.5 times higher disease incidence in unfumigated soil. Black dot on progeny tubers was reduced by pre-treatment of seed with prochloraz in fumigated soil only. With black dot infested fields, planting disease-free seed or treating seed with fungicides would not decrease disease on progeny tubers.
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  • 54
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    Potato research 41 (1998), S. 203-209 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; tuber ; blanching ; pre-drying
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Factors affecting the volume expansion of potato cubes during puffing in a fluidised bed were examined. It was found that puffing temperature, initial size, pre-drying time and puffing time influenced the degree of expansion of the cube during the puffing step. Blanching alone was found not to influence expansion, but in conjunction with the other factors it was found that blanched samples showed greater volume expansion than unblanched samples. The optimum conditions for maximum volume expansion were found to be; blanching for 80 s, cube size between 7.5–12.5 mm, pre-drying time of 90 min. at 90°C and a puffing time of 270s at 130°C.
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  • 55
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    Potato research 41 (1998), S. 211-217 
    ISSN: 1871-4528
    Keywords: in vitro tuberization ; micropropagation ; mineral nutrition ; seed potato production ; Solanum tuberosum L. ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of inorganic nitrogen nutrition on the induction and development of microtubers by cytokinin-induced tuberization was studied in four potato genotypes belonging to different maturity groups. The objective of this study was to investigate whether a reduction in total nitrogen level in the Murashige & Skoog medium would improve cytokinin-induced tuberization rate. The effect of three levels of total nitrogen (15, 30 and 45 meq) on tuberization was studied at constant (20 meq K) and varying potassium levels approximating to 5, 10 and 15 meq. Reducing the total nitrogen supply increased the number but decreased the size of nitrogen level on the rate of assimilate partitioning (harvest index) during cytokinin-induced microtuberization.
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  • 56
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    Potato research 41 (1998), S. 219-228 
    ISSN: 1871-4528
    Keywords: homozygous genotypes ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using potato parental lines homozygous at a locus or loci controlling resistance to potato leafroll virus (PLRV) can give advantages in the selection of resistant forms. In order to identify homozygous diploid clones their test-cross families were evaluated. All the clones that were test-crossed expressed resistance in primarily- and secondarily-infected plants and etiolated sprouts, and were derived from mating genotypes highly resistant to PLRV. Genotypes from test-cross families varied in resistance to PLRV, and one family was found which had only resistant genotypes, suggesting that the resistant parent of this progeny was homozygous at resistance loci. Evidence was gathered that resistance in some diploid clones may result from resistance to virus multiplication as well as restricted virus transport from leaves to tubers.
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  • 57
    ISSN: 1871-4528
    Keywords: ratio ; root-shoot ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato, wheat and sugar beet were grown in a growth chamber in tanks with flowing solutions containing K-concentrations of 1.5, 5, 15, 50 and 200 μM to find reasons for different K contents of solution needed to reach 90% maximum dry matter accumulation (external K requirement). All species showed variation in dry matter accumulation with varying K levels. To reach 90% maximum dry matter accumulation potato needed 40 μM K, whereas wheat and sugar beet needed 6.4 and 4.4 μM K respectively. These differences in external K requirement could not be explained by differences in their internal K requirement since the internal requirement of potato (7.7% K) was about one and a half times of that of sugar beet (5.4%) but its external requirement was nine times of that of sugar beet. At low K supply potato plants showed a lower influx and a reduced root growth as compared with wheat and sugar beet.
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  • 58
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; host differentials ; R genes ; multilocus genotype ; host-pathogen interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A collection of 80 isolates ofPhytophthora infestans, representing multilocus genotypes of the pathogen found in Canada from 1994 to 1996, was screened on a series of nine differential potato host cultivars to determine the physiological race of the isolates. A total of 28 pathotypes were found among the 11 genotypes present in the collection. There was a significant increase in the complexity of pathotypes from 1994 to 1996 which reflected the displacement of the US-1 genotype (mean of 2.2 host differentials infected) by the US-8 genotype (mean of 8.1 host differentials infected). Eighteen of the US-8 isolates could overcome all nine of the resistance genes tested. Virulence genes which overcame host resistance conferred by R8 were the rarest in the populations studied. The predominance of complex populations ofP. infestans in Canada implies that the introduction of cultivars with specific R genes for resistance would be an ineffective control measure.
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  • 59
    ISSN: 1871-4528
    Keywords: alcohol dehydrogenase ; phosphoglucose isomerase ; sugar assay ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Artefactual results were obtained during assay of sugars in ethanolic extracts of potato tubers with the microplate method. The problem was attributed to contamination with alcohol dehydrogenase of the commercial (yeast) phosphoglucose isomerase used in the assay. The use of phosphoglucose isomerase from another source (rabbit muscle) eliminated the problem.
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  • 60
    ISSN: 1573-5087
    Keywords: growth habit ; ipt gene ; phytohormones ; potato plants in vitro ; Solanum tuberosum L. ; tuberization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract IR68 and Dular rice cultivars were grown under ambient, 13.0 (simulating 20% ozone depletion) and 19.1 (simulating 40% ozone depletion) kJ m-2 day-1 of biologically effective ultraviolet-B (UV-BBE) for 4 weeks. Plant height and leaf area were significantly reduced by supplemental UV-BBE radiation. Greater reduction in leaf area than of plant height was observed. A decrease in indole-3-acetic acid (IAA) content and increase in peroxidase and IAA oxidase activities of UV-B treated plants in both cultivars were observed compared with ambient control. Calmodulin content also decreased after plants were treated with high supplemental UV-B for two weeks and medium UV-B treatment for four weeks. The results indicated that peroxidase and IAA oxidase activities in rice leaves were stimulated by supplemental UV-B, resulting in the destruction of IAA which in turn may cause inhibition of rice leaf growth. Although the mechanism is unclear, calmodulin is most likely involved in leaf growth.
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  • 61
    ISSN: 1573-5087
    Keywords: growth habit ; ipt gene ; phytohormones ; potato plants in vitro ; Solanum tuberosum L. ; tuberization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nodal cuttings of in vitro grown potato plants (Solanum tuberosum, cv. Miranda) were transformed by a vector plasmid carrying ipt gene of Agrobacterium tumefaciens. From the initial teratoma stage 5 clones of transgenic plants (1, 2, 11, 13 and 15) were obtained, which displayed in varying degree shortening of the internodes, decrease of the leaf size, decrease of apical dominance and poor rooting. In addition, two of the clones (11 and 13) showed increased stolon and tuber formation. In all these clones the endogenous level of free cytokinins (CKs) was increased: from 40% in clone 11 to almost 300% in clone 1. Also free indole-3-acetic acid (IAA) level was increased, but to a lower degree; the maximal increase was 160% (clone 13). Applied kinetin or IAA (1 mg·l-1) strongly suppressed root and tuber formation in clones 11 and 13, although they did not affect or even stimulated these processes in control plants. For control plants the minimal medium sucrose concentration necessary for tuber initiation was 6% whereas in clone 11 plants 2% was sufficient. Different distribution of endogenous CKs and IAA was observed in clone 11 and control plants. The highest CK content was found in transgenic plants in stems and in controls in leaves. In clone 11 plants abscisic acid (ABA) level was significantly increased in comparison to the control throughout the cultivation period. Ethylene formation was strongly increased the first week after the subcultivation and later on the difference between transgenic and control plants rapidly diminished. Reactions of clone 11 plants to red (RL) and blue light (BL) were similar to reactions of control plants. In RL clone 11 plants were tall and thin with stunted leaves; in BL they had a teratoma-like appearance and formed a very high number of tubers. The role of hormones in these changes in growth and tuber formation is discussed.
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  • 62
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    Journal of agricultural and environmental ethics 10 (1997), S. 249-267 
    ISSN: 1573-322X
    Keywords: Animals ; Asia ; consciousness ; Australia ; Hong Kong ; India ; Israel ; Japan ; New Zealand ; The Philippines ; Russia ; Singapore ; Thailand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract The interactions between humans, animals and the environment have shaped human values and ethics, not only the genes that we are made of. The animal rights movement challenges human beings to reconsider interactions between humans and other animals, and maybe connected to the environmental movement that begs us to recognize the fact that there are symbiotic relationships between humans and all other organisms. The first part of this paper looks at types of bioethics, the implications of autonomy and the value of being alive. Then the level of consciousness of these relationships are explored in survey results from Asia and the Pacific, especially in the 1993 International Bioethics Survey conducted in Australia, Hong Kong, India, Israel, Japan, New Zealand, The Philippines, Russia, Singapore and Thailand. Very few mentioned animal consciousness in the survey, but there were more biocentric comments in Australia and Japan; and more comments with the idea of harmony including humans in Thailand. Comparisons between questions and surveys will also be made, in an attempt to describe what people imagine animal consciousness to be, and whether this relates to human ethics of the relationships.
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  • 63
    ISSN: 1871-4528
    Keywords: minimum stomatal resistance ; soil water ; Solanum tuberosum L. ; photosynthetic photon flux density ; vapour pressure deficit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Leaf stomatal resistance varies considerably in response to changes in environmental conditions. Understanding cultivar differences in the response of stomata to these conditions is important for effectively simulating water-use and growth. In this study the stomatal resistance of three field grown potato (Solanum tuberosum L.) cultivars Atlantic. Monona and Norchip was observed in response to photosynthetic photon flux density, leaf to air vapour pressure difference and root zone available soil water. Substantial variation in stomatal resistance was accounted for by functions of the environmental variables for both Monona and Norchip. The explained variation for Atlantic was considerably less. Light saturation occurred near 500 μmol m−2 s−1. Increasing stomatal resistance above 1000 μmol m−2s−1 was also observed but is likely an artifact of stomatal closure on days in which mid-day leaf water potentials dropped below critical levels. Although the leaf presumably insufficient to obtain substantial effects. The soil water status was partially linked to the average 1000 h to 1500 h stomatal resistance on clear, sunny days. Significant differences were observed among the cultivars in the response of stomata to changes in the available soil water.
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  • 64
    ISSN: 1871-4528
    Keywords: fungicide resistance ; cultivars ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The majority of isolates ofFusarium avenaceum caused dry rot on potato tubers; many were as pathogenic asF. coeruleum. Pathogenicity ofF. avenaceum was not related to the plant species from which the isolates originated. Tubers of potato cv. Cara were more susceptible than those of cvs Romano or Maris Piper. Temperature (5–15° C) had no effect on lesion depth but lesions tended to be slightly wider at the lowest temperature. Two isolates (of 61) from wheat and white lupin had a moderate level of resistance to thiabendazole. The results are discussed in relation to the control of dry rot in stored potato tubers.
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  • 65
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; Globodera rostochiensis ; G. pallida ; soil type ; soil pF ; cultivar ; damage ; tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Data from five long-term field experiments on sandy and sandy peat soils were analyzed for the effect of soil type on damage caused by potato cyst nematodes to potato crops. It was shown that the water retention of the topsoil and the subsoil largely determined the potential yield level in the different fields. According to Oostenbrink's equation the slopes of the curves are steeper with increasing yield level, indicating that the higher the potential yield level, the higher the absolute yield loss. However, when yield was expressed as percentage of maximum yield, yield reduction related to potato cyst nematode density did not differ significantly, except when tolerant cultivars were grown. Increasing tolerance of cultivars to nematode damage was reflected in less steep slopes of the yield/nematode density curves.
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  • 66
    ISSN: 1871-4528
    Keywords: agronomy ; breeding ; physiology ; pathology ; virology ; engineering ; utilization ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The highlights and achievements of 40 years of potato research in agronomy; genetics, breeding and varietal assessment; physiology; pathology (including nematology); virology; engineering; and utilization are reviewed and the prospects for new developments are analysed. In all disciplines progress has been impressive and has changed the potato industry dramatically. Yet, some old problems remain unsolved (Phytophthora infestans, blackspot) or are returning (cyst and other nematodes) and new problems have arisen (e. g. volunteer potatoes, new strains of PVY, virulent cold-resistant strains ofPseudomonas solanacearum). The potato industry faces new challenges from society (environmentally safe production) and the consumers (improved quality). Farmers, researchers and processors have access to many new exciting techniques, tools and opportunities, such as precision farming, genetic engineering, molecular detection and analytical techniques, integrated chain management, novel preservation and processing methods. The problems and opportunities both warrant a continued effort in potato research.
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  • 67
    ISSN: 1871-4528
    Keywords: skin resistance ; temperature ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Chambers were designed to simulate environmental conditions present in commercial potato stores and were used to introduce condensation to tubers infected with silver scurf (Helminthosporium solani). The electrical resistance measured across the skin of tubers from the top of the chambers was consistently lower than that from the bottom of the chamber, and this was reflected in an increased number ofH. solani spores produced by the top tubers. An increase in the length of the condensation period resulted in the skin resistance remaining lower for a longer period and also resulted in higher number of spores. Tubers held at relatively high ambient temperatures (15 °C) required a shorter period of condensation to cause an increase in spore count. This investigation highlighted the need for close monitoring of store environments so that condensation events can be quickly identified and the tubers dried.
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  • 68
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    Potato research 40 (1997), S. 1-4 
    ISSN: 1871-4528
    Keywords: scientific journal ; professional organization ; agronomy ; crop science ; research ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato Research started as the “European Potato Journal”, the official journal of the European Association for Potato Research, in 1958, was re-named “Potato Research” in 1970 (Volume 13) and has published mainly original scientific contributions in 39 volumes. The authorship has become increasingly international and currently writes predominantly in English, although papers are also offered in German or French. Topics covered have seen clear changes over volumes, mainly reflecting trends and break-throughs in (potato) science.
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  • 69
    ISSN: 1871-4528
    Keywords: water stress ; photosynthesis ; stress index ; dry matter partitioning ; tuber quality ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A lysimeter experiment was performed to study the optimal allocation of limited water supply in potatoes. Irrigation regimes equal to 40, 60 and 80% of maximum evapotranspiration (ET) were evenly applied over the crop cycle. Other treatments involved withholding 80 mm of irrigation, based on ET, beginning at each of three designated growth stages (tuber initiation, early and late tuber growth). An irrigated control treatment, restoring the entire ET, was included for comparison. Continuous drought stress reduced photosynthesis as irrigation volumes were reduced. Plant biomass and tuber yield decreased almost proportionally to water consumption, so that WUE was roughly constant. N uptake was highest in the control and in 80% ET treatment. Withholding water during tuberisation severely hindered plant physiological processes and penalized tuber yield. Reductions in photosynthesis, total biomass and yield were the greatest when drought was imposed during tuber initiation. The earliest stress resulted in the lowest WUE and N uptake. A new crop water stress index (SI) was proposed, which combines atmospheric demand for water and canopy temperature.
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  • 70
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    Potato research 40 (1997), S. 35-46 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; in situ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The first large scale outdoor measurements of potato (Solanum tuberosum L.) stem water potential (SWP) usingin situ temperature-corrected stem psychrometers were performed on three cultivars common to Ontario, Canada (Atlantic, Monona and Norchip). Potato plants were examined under three soil water treatments which included rainfed, irrigated and stressed through the use of rain shelters. Cultivar variations in SWP under both irrigated and rainfed conditions were slight (P〉0.05), although some significant (P〈0.05) differences in mid-day SWP were evident, especially under conditions conducive to high atmospheric water demand. Under these conditions. cv. Atlantic maintained the lowest SWP with cv. Monona highest and cv. Norchip was intermediate. Few differences in pre-dawn SWP were detected between the three cultivars under well watered and rainfed conditions. Under low soil water, significant differences (P〈0.05) were observed. The similar trend of Atlantic maintaining the lowest SWP with Monona the highest was consistent.
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  • 71
    ISSN: 1871-4528
    Keywords: potato cultivar ; storage ; cooking type ; descriptor ; dry matter ; Principal Component Analysis (PCA) ; Regression Analysis ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The texture of steam-cooked potatoes from ten cultivars was sensory evaluated after two, four and nine months storage for three consecutive years. The sensory data were analyzed using Principal Component Analysis (PCA) and regression analysis. PCA revealed that the first two principal components explained 95% or more of the variance between the data. The first principal component was dominated by the descriptorsmealy (M)/crumbly (A/M) on the positive side and the descriptorswaxy (A/M) on the negative side. The descriptorfirm (M) had a high positive loading on the second principle component. Regression analysis showed that cultivar effects dominated storage effects. Based on these data, a proposal is made to divide the ten cultivars in four groups which differ for the descriptorsmealy (M)/crumbly (A/M), waxy (A/M) andfirm (M). During storage, potato texture became morewaxy (A/M) andsticky (A/M) with respect to both appearance and mouthfeel. Other changes in texture as a result of storage were strongly cultivar dependent.
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  • 72
    ISSN: 1871-4528
    Keywords: suberin ; suberization ; lenticel penetration ; Erwinia carotovora ssp.atroseptica ; tuber development ; Solanum tuberosum L. ; biological control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using the fluorescent lipid stain fluorol yellow 088 to detect suberin in tubers, the numbers of layers and the thickness of the suberized cells in the periderm and within the lenticels showed changes after the early stage of tuber formation. These changes as tubers matured were affected by cultivar and soil moisture conditions. Penetration of the surface tissues of tubers by the water soluble stain safranin O was confined to the lenticles. Although the zone of suberized cells in lenticels acted to some extent as a barrier to penetration, there was not a clear relationship between permeability and suberization. Stored tubers showed thicker suberin berriers in lenticels than freshly harvested tubers. When lenticels of stored tubers proliferated the suberin barrier was disrupted but a further thick suberin barrier formed after exposure to air for two weeks. The findings are discussed in relation to biological control ofErwinia by antagonistic bacteria.
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  • 73
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    Potato research 40 (1997), S. 191-214 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; propagation by sexual seed ; vegetative propagation ; socio-economic potential ; potatoes in the tropics ; breeding ; true potato seed (TPS)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The vast majority of cultivated potatoes are vegetatively propagated, outbred autotretraploids. Disease problems dominate the maintenance of vegetative stocks. There have been recent proposals to propagate the crop by sexual seed in order to evade some of those disease problems. Some success has been achieved but controlled crosses are necessary to avoid inbreeding depression and seed propagation is not as cheap or simple as had been hoped. The idea has evoked wide interest throughout the tropics and has had some (and increasing) practical impact on China, India and Vietnam. There is a strong tendency to use ‘tuberlets’ borne on crowded nursery plants rather than to grow true seedlings. Some seedling families have looked locally attractive but it is not always realised that to use them implies the abandonment of about half the genetic variation, a heavy price to pay for disease avoidance. There is emerging recognition that vegetative and seed propagation are complementary rather than competitive and that good breeding programmes will therefore serve both. This review concentrates upon genetic/plant breeding aspects of propagation by seed, a subject hitherto largely neglected in the literature.
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  • 74
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; Helminthosporium solani ; Colletotrichum coccodes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato seed tubers infected withHelminthosporium solani andColletotrichum coccodes were treated with prochloraz (as Omega 450 g a.i. l−1 EC) and/or prochloraz managanese chloride (as Octave 25 g/kg DP), and planted in two separate fields not previously cultivated with potatoes. Dipping seed 28 d prior to planting in 2,4 or 6 g a.i. l−1 Omega, and dusting with 500 or 750 g Octave per 100 kg seed significantly reduced the incidence of silver scurf and black dot on progeny tubers. In one field, the higher concentrations of Omega and Octave gave slightly better control than the lower Omega concentrations. At the second site, postharvest application of 4 g a.i. l−1 Omega or 750 g Octave per 100 kg seed limited development of both diseases on progeny tubers, with slightly better control when an additional Octave dusting was applied 14 d before planting. None of the prochloraz treatments adversely affected plant stand or tuber yield.
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  • 75
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    Potato research 40 (1997), S. 229-235 
    ISSN: 1871-4528
    Keywords: potato ; Solanum tuberosum L. ; bacteria ; dispersal ; field trial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plant-to-plant transmission of the bacterial ring rot (BRR) pathogen,Clavibacter michiganensis subsp.sepedonicus (Spieck. et Kott.) Skapt. et Burkh. of potato (Solanum tuberosum L.), was studied in field trials over a three year period. Healthy and infected seed tubers were planted 35 cm apart. In one treatment, a subsurface barrier was placed between the healthy and infected seed tubers separating the root systems of neighbouring plants. In this treatment, none of 216 plants grown from healthy seed tubers was infected at harvest, as determined by indirect immunofluorescence antibody staining (IFAS) with monoclonal antibodies. In the other treatment, no subsurface barrier was used. In this treatment, two of 368 plants (0.5%) grown from healthy seed tubers were infected at harvest. It is concluded that plant-to-plant transmission may occur but at very low frequency, and is unlikely to play a significant role compared with the potential of transmission by shared potato handling equipment.
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  • 76
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    Potato research 40 (1997), S. 251-266 
    ISSN: 1871-4528
    Keywords: LAI ; canopy expansion ; radiation use efficiency ; Solanum tuberosum L. ; soil water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth and duration of crop leaf area determines the amount of solar radiation intercepted by the canopy and therefore influences the extent of photosynthesis, evaporation, transpiration and final dry matter yield. The objective of this study was to develop cultivar specific relationships to estimate the daily leaf area index (LAI) for the potato crop (Solanum tuberosum L.) that included the effects of available soil water. The model is divided into three LAI growth stages, the durations of which are partially related to potato heat units (PHU). The LAI in the first stage is estimated from a cultivar specific leaf area-based radiation use efficiency index with a soil water reduction factor. The second stage involves the maintenance of a constant LAI with the duration related to both PHU and a soil water index that can accelerate senescence. The final stage includes a decrease in the LAI from a maximum to zero in response to a cultivar specific PHU accumulation. Model simulations compared favourably with independent LAI measurements obtained with a LI-COR plant canopy analyzer over two seasons.
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  • 77
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    Potato research 40 (1997), S. 267-276 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; transpiration estimates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A heat balance method for measuring sap flow in actively growing plants was tested on three potato (Solanum tuberosum L.) cultivars Monona, Norchip and Atlantic. The method is based on applying a known heat source to the stem of an intact plant: by measuring the axial and radial heat dissipation the magnitude of sap flow can be quantified. The technique shows promise as 24 h accumulated sap flow measurements were highly correlated (r2=0.935, n=36) with gravimetric measurements of transpiration and produced an average root mean square error (RMSE) of 20 g d−1. The accuracy of the method improved as daily accumulated flow increased. Under low daily flow rates overall RMSE increased, supporting the need for a heat storage term in the heat balance equation. Short term flow rates were less significantly correlated (r2=0.764, n=1572) and produced an average RMSE of 2.89 g h−1. Identification of these short term flow rates during high flow periods may be difficult. The presence of water potential gradients and the likelihood of re-hydration during high flow events may have also affected the short term performance of the method. At low flow rates, many different errors may be introduced.
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  • 78
    ISSN: 1871-4528
    Keywords: light ; temperature ; stolon growth medium ; below ground nodes ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five experiments studied the effects of shading, temperature and stolon growth medium on stolon and tuber formation. The lowest numbers of stolons and tubers were produced at the higher and lower nodes. Numbers of primary stolons and tubers were relatively stable across nodes and treatments and variation in total numbers was largely due to changes in numbers of lateral and branch stolons. In the field, 70% shading reduced numbers of stolons and tubers by reducing lateral and branch stolons, while in hydroponics, 45% shading and temperatures at initiation had no effect on tuber numbers. In both environments later planting tended to reduce numbers of stolons and tubers. There were large effects of the physical environment, with compost producing three times as many tubers as dry vermiculite. The potential number of tubers may be determined by the number of stolons and the environmental conditions affecting stolon formation and development.
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  • 79
    ISSN: 1871-4528
    Keywords: Globodera rostochiensis ; G. pallida ; tolerance ; yield forecasting ; damage ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary To describe the relationship between nematode density and yield, four equations of increasing complexity are found in the literature. They concern a linear regression curve, a log linear regression curve, a regression curve based on an exponential relation and an inverse linear regression curve. The yield to PCN density responses of these equations are shown on a linear and on a logarithmic scale. Considerable differences are shown in the way they respond to the same data. None of these equations is able to explain or forecast yield decrease caused by potato cyst nematodes.
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  • 80
    ISSN: 1871-4528
    Keywords: Globodera rostochiensis ; G. pallida ; Solanum tuberosum L. ; cultivars ; maturity-class ; precipitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In three seasons, 21 potato cultivars, ranging in maturity from very late to mid-early, were grown on sandy and sandy peat soils naturally infested with potato cyst nematodes. The levels of infestation ranged from very low to very high. Between growing seasons, weather conditions differed greatly from very dry and hot to rather cool and wet. The data obtained were used to establish the effect of different growing seasons on the initial nematode density — tuber yield relationship and to evaluate the fit of the several equations describing this relationship. Over the three experimental years, the equations elaborated by Oostenbrink (1966) and Elston et al. (1991) described this relationship with the most accuracy and consistency. There were high year effects on yield, but as tolerance was not affected by the yearly fluctuations, the results suggest that testing for tolerance can be adequately done in any single year.
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  • 81
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; Globodera pallida ; soil type ; cultivar ; damage ; tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The sandy and sandy peat soils of the northeast of The Netherlands are mineralogically very poor and acid. Independently of potato cyst nematode infestations, there is a considerable negative effect of increasing soil pH on potato tuber yield. Potato cyst nematode infections aggravate the effect of soil pH in depressing yield. It was shown that both potato cyst nematode infection and increasing soil pH values reduce the buffering capacity of the root system. Testing for yield as well as for tolerance to potato cyst nematode infection should be made at a range of soil pH values, similar to those found in the target area. This refers to active breeding programmes as well as to the selection of cultivars for yield trials.
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  • 82
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; disease screening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crosses were made between six parents differing in susceptibility to skin spot and glasshouse-grown tubers from true seedlings of the nine resulting progenies assessed for resistance to the disease. The assessment was evaluated by a two-year comparison with the behaviour of field-grown tubers of the progenies and their parents. The correlations between progenies in the glasshouse and field were r=0.911 and 0.753 and those between the mid-parental values and the progeny means were high (r〉0.864), indicating that parental differences were heritable. It is concluded that correlations were sufficient for glasshouse-grown tubers to be used for assessing large numbers of progenies in genetical studies and for eliminating highly susceptible progenies at an early stage in a breeding programme.
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  • 83
    ISSN: 1871-4528
    Keywords: fungicides ; Solanum tuberosum L. ; Trichoderma harzianum ; watery wound rot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of fungicides and the antagonistic fungusTrichoderma harzianum (Th) on the potato leak pathogenPythium aphanidermatum (Pa) was investigated in vitro. Rot was reduced by 73% to 89% when tubers inoculated withPa were treated by immersion in solutions (0.5% w/v) of the fungicides Ridomil MZ58. Dithane M45. Manebe 80 and Tachigaren 360. Complete protection was achieved by dusting inoculated tubers with Ridomil MZ58 diluted in kaolin at a final concentration of 0.1% (w/w). The biological treatments of dipping inoculated tubers in a conidial water suspension ofTh at 108 conidia/ml or of dusting with a mixture of a barley culture ofTh and kaolin to a final concentration of 2×109 conidia/g were as effective as the chemical treatments. Control was still effective when inoculated tubers were treated with Ridomil MZ58. Tachigaren 360 or the conidial suspension ofTh after 24h incubation at 25°C.
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  • 84
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; in vitro propagation ; seed production ; green microtubers ; white microtubers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The performance of potato crops raised from two types of microtuber, green (induced in light-dark photoperiod) and white (induced in continuous darkness), of 18 genotypes was studied for 22 morphological and agronomic characters. Mean squares due to microtuber type, genotype and their interaction were mostly significant for various characters. Green microtubers were better than white microtubers for subsequent crop performance for most of the characters including tuber yield and general impression. This was mainly due to more eyes and shorter dormancy in green microtubers than in white microtubers. The in vitro system of microtuberization by serial culture of axillary buds in separated nodes, as used in the present study, was found to be genetically stable for the propagating material.
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  • 85
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; watery wound rot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The susceptibility of 21 potato cultivars to leak (watery wound rot) caused byPythium aphanidermatum was compared. Whole tubers were inoculated after wounding by dipping into a water suspension of 103 oospores/ml and incubated for 3 days at 25°C. The mean penetration of tissue calculated from the recording of lesion width and depth and the qualitative assessment of rot extension were highly correlated. For 10 of 12 cultivars tested at least twice, consistent reactions were obtained: cvs Korrigane. Superstar, Safrane and Yesmina were moderately susceptible, whereas Atlas, Diamant, Mondial, Obélix, Spunta and Timate were susceptible to highly susceptible. Tubers originated from two locations and/or two cropping seasons: thus, the performance of these cultivars under different production conditions reveals a satisfactory level of agreement with the test. Moreover, ratings assigned are supported by field or store observations.
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  • 86
    ISSN: 1871-4528
    Keywords: minitubers ; in vitro plants ; autumn-winter cycle ; spring-summer cycle ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two culture systems for propagating first generation potatoes were compared; the traditional system used a peat/sand mixture with mineral fertilizer, and hydroponic culture used perlite and nutrient solution. Total production and the number of tubers obtained using the hydroponic system were significantly higher than using the traditional culture system. Tuber yields from in vitro plants and minitubers depended upon time of year. During the autumn/winter cycle yield from minitubers was double that from in vitro plants, whereas the reverse was true during the spring/summer cycle. Four hydroponic test cultures were carried out to study the influence of seed density. The number of tubers obtained increased significantly with seed density but there was no decrease in the number of large-diameter tubers.
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  • 87
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    Potato research 40 (1997), S. 439-453 
    ISSN: 1871-4528
    Keywords: vitroplantlets ; microtubers ; minitubers ; genetic stability ; storage and physiological age ; field performance ; seed production programmes ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The production of large volumes of vitroplantlets and greenhouse tubers for increasing the rate of multiplication at the start of seed programmes provides the opportunity of reducing the total number of field generations grown before the seed moves into commerce. This implementation is especially useful for countries where high quality potato seed tubers cannot be produced because there are no vector-free production areas. This review covers the following steps: a) laboratory production of microplantlets and microtubers; b) minituber production in the glasshouse; c) storage and dormancy of micro- and minitubers; d) field performance of micro- and minitubers compared with conventional seed tubers; e) incorporation of the mentioned propagules in seed production systems. Many optimized protocols are already available for propagating plantlets, inducing microtubers and obtaining minitubers in the glasshouse at all periods of the year. Advanced molecular approaches techniques (RFLP and RAPD) to detect genetic variation in the progeny of these propagules have been described. Investigations carried out in this field have shown genetic stability, with the propagules usually reproducing plants true-to-type and tubers without deviants. By contrast, variations were demonstrated in DNA extracted from old suspension cell culture. Field trials assessed a lower yield potential crops from in vitro propagules compared with conventional seed tubers., mainly due to slow early crop development and the failure of plants caused by early stress after emergence. This may cause problems when the growing season is short because of the necessity for planting late to avoid night frosts and the mandatory haulm killing dates, common in many seed producing areas. Strategies for improving the field performance of micro- and minitubers are discussed. The most promising crop husbandry techniques appear to be: a) using tubers of a suitable physiological age, properly presprouted and encapsulated; b) optimizing the time application of fertilizer and irrigation, and c) using floating films. Outside the classical seed tuber areas of Northern Europe where the length of the growing period for pre-basic seed is usually not more than 80 days, the growing season is long enough to obtain reasonable yields even from micro- and minitubers.
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  • 88
    ISSN: 1572-9788
    Keywords: complementation ; granule-bound starch synthase ; Solanum tuberosum L. ; expression ; transgenic inheritance ; ploidy level
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Theamylose-free (amf) potato mutant can easily be complemented through introduction of the wild-type gene coding for granule-bound starch synthase (GBSS). After iodine staining the starch of theamf mutant is red whereas that of the wild type and the complementedamf mutant is blue. The level of complementation of selected transformants and their sexual off-spring after backcrossing withamf was investigated using sporophytic tuber cells and gametophytic microspore cells. Two diploid and two tetraploid transformants with full complementation demonstrated the expected segregation patterns of 1:1 (one active insert) or 3:1 (two independently segregating active inserts) in the microspores and in the F1 offspring based on staining of tubers. All expected genotypes in the F1 generation were found, based on microspore segregation patterns of the individual F1 plants. Two transformants with partial complementation (mixed phenotypes) were investigated. One of them, B1, was tetraploid and duplex for the GBSS insert, which had originated through mitotic doubling of the transformed diploid cells. In the F1 generation three phenotypic classes were found:amf, fully complemented and partially complemented. The latter two classes exist independently of a simplex or duplex gene status. The second transformant with partial complementation, B10, appeared to have a complex molecular composition. One cluster of five transgenes caused the partial complementation. Fully and partially complemented phenotypic classes were found after crossing B10 with theamf mutant. Indications were found that the ploidy level of the tissue in which the genes were introduced and expressed played an important role. Firstly, partial complementation was found after transformation of the diploid and not of the tetraploidamf genotypes. Secondly, the level of complementation was higher in tissue with lower ploidy levels, as illustrated by the colour of the starch inin vitro tubers (2x–4x cells) versus field-grown tubers (16x–64x).
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  • 89
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    European journal of plant pathology 102 (1996), S. 317-324 
    ISSN: 1573-8469
    Keywords: Globodera spp. ; Solanum tuberosum L. ; drought tolerance ; variety selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In field trials during three years respectively 18, 22 and 57 potato genotypes were grown on soils moderately or heavily infested with potato cyst nematodes (Globodera pallida) after soils were fumigated or not. Nematode infection increased leaf calcium contents but genotypes that were relatively tolerant of potato cyst nematodes (Globodera pallida) had lower leaf calcium concentrations on a particular sampling date. Tolerance of potato cyst nematodes was closely linked to genotype lateness and may be related to tolerance of drought. When using the method in plant breeding and screening for genotypes with tolerance, plants may be planted in infected or in uninfected soils, without influencing the outcome. The relationship between tolerance and calcium contents was clearest when sampling took place towards the end of the growing season when the variability was at it highest with a good distinction between newly formed leaves in late maturing genotypes and aged leaves in early maturing cultivars.
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  • 90
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    Potato research 39 (1996), S. 411-427 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; disease ; quality ; carbohydrates ; starch ; carbon partitioning ; development ; stress ; molecular farming
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Transgenic potato plants are being generated world-wide to assess the impact of transgene expression (sense and/or antisense) on parameters as diverse as yield, quality, stress physiology and pest/disease resistance. Strategies for alternative uses of the potato crops by diverting metabolism into high value products or by expressing recombinant proteins are also being explored. Results obtained are often surprising although the science of plant molecular biology is relatively young. Whilst the outcome of any transgenic approach is by no means certain, this review provides sufficient examples to illustrate the versatility and applicability of transgenic biology in potato improvement.
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  • 91
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    Potato research 39 (1996), S. 507-513 
    ISSN: 1871-4528
    Keywords: genebank ; genetic stability ; in vitro culture ; shoot-tips ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A simple freezing method has been developed for the cryopreservation of an in vitro collection of old potato varieties. More than 125 varieties or genotypes have been frozen and stored in liquid nitrogen. Most varieties have high survival rates, averaging 80%. Plant regeneration rates were lower and averaged 40%. Plants have been obtained from all varieties or genotypes frozen and the phenotypes of regrown plants matched very well those of the unfrozen stock plants. However, one plant was found which was probably polyploid and five plants had poor growth. In addition, the genetic stability of 161 regrown plants was checked by flow cytometric measurements and DNA-fingerprinting. In the 161 tested samples neither polyploid plants nor unusual banding patterns were found.
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  • 92
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    Potato research 39 (1996), S. 515-522 
    ISSN: 1871-4528
    Keywords: Erwinia carotovora ; soft-rot ; tuber tissue ; cell lysis ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the cell wall matrix of higher plants, cellulose fibrils are embedded in a network of pectic substances, hemicelluloses and structural proteins. But plant pathogenicErwinia carotovora bacteria secrete a mixture of enzymes including pectinases, cellulases and proteases to degrade the cell walls of host plant tissue. Tuber tissue of 31 potato genotypes was exposed to a mixture ofErwinia enzymes before the degree of cell wall lysis was determined by a Neutral-red vital staining method. The data indicate that the resistance of cell walls to the action ofErwinia enzymes differed significantly among the potato cultivars and clones. The decrease in cell viability caused by the action of enzymes ranged from 7% for cv. Maxilla to 〉46% for cv. Arnika. An increased rate of N-fertilization coincided with enhanced susceptibility of cell walls to the action ofErwinia enzymes. There was a correlation between the amount of cell wall substances in tuber tissue and the resistance of cell walls to enzymic degradation.
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  • 93
    ISSN: 1871-4528
    Keywords: CO2 ; O2 Record ; Saturna ; Hermes ; sprout length ; fry colour ; sugar ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three crisping potato cultivars, Record, Saturna and Hermes, were stored at 5 or 10°C in gas mixtures of either 0.5% CO2 and 21.0% O2 (control) or 9.4, 6.4, 3.6 or 0.4% CO2 all combined with 3.6% O2. There was almost complete sprout inhibition, low weight loss and maintenance of a healthy skin for all cultivars stored in 9.4% CO2 with 3.6% O2 at 5°C for 25 weeks. When tubers from this treatment were stored for a further 20 weeks in air at 5°C the skin remained healthy and they did not sprout. The fry colour of crisps made from these potatoes was darker than the industry standard but when they were reconditioned, tubers of cv. Saturna produced crisps of an acceptable fry colour while crisps from the other two cultivars remained too dark. Reducing sugar levels were related to fry colour both after storage and after reconditioning. The other gas combinations and the controls did not have the same effect on sprouting and none of the controlled atmosphere treatments controlled sprouting at 10°C.
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  • 94
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    Potato research 39 (1996), S. 533-540 
    ISSN: 1871-4528
    Keywords: radiation ; interception ; light ; potato ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two methods for measuring the proportion of crop ground cover were compared. Both employed a rectangular grid of cords fitted in a frame at regular distances. One was based on the proportion of rectangles in which green area occupied more than half, and the other was based on the proportion of intersects coinciding with green area. The second method was accurate under all circumstances. Whereas the first method was found to give biased records when the smallest details in the canopy were small compared to the grid cell size. A theoretical estimate of the standard error of the intersect method was derived for homogeneous canopies and confirmed by experimental data.
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  • 95
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    Potato research 39 (1996), S. 541-550 
    ISSN: 1871-4528
    Keywords: late blight ; fluazinam ; maneb-fentinacetate ; rainfastness ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of the intensity of rain and the interval between application of fungicides and the onset of rain on the efficacy of maneb-fentinacetate and fluazinam againstPhytophthora infestans in potato was evaluated in pot trials and field trials. In the pot trials a clear wash-off of maneb-fentinacetate was observed when simulated rain was applied 4 hours after plants had been sprayed with the fungicide. The retention of fluazinam deposits was higher and in some trials the efficacy in the bioassay was even increased by rain, probably by redistribution from the spray deposits. When 10 mm of simulated rain was applied 4–6 days after application of the fungicides, no decrease in the efficacy was observed with both fungicides. In the field trials fluazinam also showed a better retention of deposits compared with maneb-fentinacetate.
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  • 96
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    Potato research 39 (1996), S. 551-559 
    ISSN: 1871-4528
    Keywords: fungicides ; Phytophthora infestans ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Synergism between fungicides could be used to reduce the application dosage. Synergism between cymoxanil and mancozeb was studied using potted potato plants under field conditions. In three experiments the preventive efficacy of the fungicides and fungicide mixtures in the treatments were studied using a bio-assay. Potato plants were sprayed with the fungicides or fungicide mixtures. Leaves were picked from the potato plants with regular intervals after fungicide application. The efficacy of the fungicides was studied in a bioassay by inoculating the leaves withPhytophthora infestans zoosporangia. The Abbot method was used to show synergistic effects of the cymoxanil/mancozeb mixtures tested. In two of three experiments synergy of the fungicide mixtures was found.
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  • 97
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    Potato research 39 (1996), S. 43-50 
    ISSN: 1871-4528
    Keywords: Amylose ; cultivar ; glucose-6-phosphate ; granule size ; Solanum tuberosum L. ; tuber size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato starch quality parameters (amylose concentration, glucose-6-phosphate content and granule size) were analyzed in relation to the harvesting date and tuber size of four cultivars. A significant increase in the glucose-6-phosphate content and granule size of starch was observed during tuber growth, whereas the amylose concentration was constant. Granule size increased markedly, whereas glucose-6-phosphate content showed slightly increasing values with increasing tuber size. Amylose concentration showed no correlation with tuber size. We conclude that the changes in the granule size, glucose-6-phosphate and amylose content of potato starch during growth are independent of each other.
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  • 98
    ISSN: 1871-4528
    Keywords: seedling density ; Solanum tuberosum L. ; tuber size ; 4X×4X ; 4X×2X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of varying plant population on the production of tubers from potato seedlings was investigated during 1992 and 1993 using three different plant densities: 35, 70, 100 plants/m2. Increasing the plant population significantly increased the number of tubers produced, but no significant difference was found between the plant densities of 70 and 100 plants/m2. The seedling tubers produced in nursery beds were tested in the field for ware potato production using different sized tubers. The best performances were obtained using tubers 30–40 mm. Significant differences were evident among the 7 true potato seed (TPS) families used, but data grouped by type of cross did not indicate any significant difference between 4X×4X and 4X×2X crossing groups.
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  • 99
    ISSN: 1871-4528
    Keywords: Silver scurf ; Solanum tuberosum L. ; Mertect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Survival of the pathogen causing silver scurf of potato (Helminthosporium solani) in Alberta soils was studied by evaluatingH. solani infection in the progeny ofH. solani-free nuclear seed potato tubers planted in fields where potatoes had never been grown or were grown 1, 2, 3, or 4 years previously. Daughter tubers from all the fields developed silver scurf lesions, andH. solani was isolated from infected tubers. This is the first report of survival ofH. solani in Alberta soils. Soil-borne inoculum appears to have a role in the epidemiology of the disease and in the introduction of the pathogen into silver scurf-free potato seed stock. Of 31 plant species tested, only potato was found to be a host ofH. solani. Most of theH. solani isolates from north central Alberta were more sensitive to thiabendazole than those from southern Alberta, where thiabendazole is much more commonly used.
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  • 100
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    Potato research 39 (1996), S. 63-68 
    ISSN: 1871-4528
    Keywords: senescence ; protein ; chlorophyll ; Solanum tuberosum L. ; Calonyction aculeatum (L.) ; House
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Foliar sprays of calonyctin, a natural plant growth regulator extracted from the leaves ofCalonyction aculeatum (L.) House, at a concentration 0.05 activity units, increased the tuber yield of six potato (Solanum tuberosum L.) cultivars by 7–19% (mean 15%) during 1993 and 1994. Physiological differences between calonyctin-treated and untreated potato plants were usually detected 30–40 days after spraying them with calonyctin. The chlorophyll content of treated potato leaves increased, and the chlorophyll a:b ratio decreased. By 40 days after treatment the root oxidizability and soluble protein contents of different parts of the plants also increased. Their subsequent decline as the plants aged was inhibited by calonyctin. It is concluded that calonyctin delayed the senescence of potato plants and increased tuber growth at later stages of development.
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