ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Sterols, tocopherols, retinols and carotenes
;
Dairy products
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary We have previously described a normal-phase HPLC method for the simultaneous determination of α, β, γ and δ tocopherol, thecis andtrans isomers of retinol, and total carotenes in different Italian cheeses and milk. In this paper we describe a reversed-phase HPLC method using solely methanol as the mobile phase for the determination of α-tocopherol, β-+γ-tocopherols, total retinols, cholesterol, β-sitosterol, campesterol and stigmasterol, and α- and β-carotene. The method developed has been applied to some new dairy products such as natural Quark, with olive, basil or banana and to processed cheese with tomato and the results have been compared with the corresponding results obtained by the normal-phase method. The conclusion is that more complete evaluation of the tocopherol, retinol, carotene and sterol content of dairy products, especially when vegetables and fruits are present, is necessary and can be obtained using both the normal- and reversed-phase HPLC methodologies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02272829
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