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HPLC-continuous-flow fast atom bombardment mass spectrometry (HPLC-CFFAB) — a convenient method for the analysis of bile acids in bile and serum

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Summary

In order to analyse bile acids in biological matrices, e.g. rat bile and human serum, high performance liquid chromatography (HPLC) was coupled to continuous-flow fast atom bombardment mass spectrometry (CFFAB-MS). A gradient elution system which had already proved to be well suited for the quantitative determination of conjugated bile acids in bile was modified to allow HPLC-CFFAB-MS-coupling. Due to the sensitivity of this coupling method it is possible to obtain more information about the biliary bile acid pattern and species-specific secondary bile acids. Furthermore, we were able to identify obviously unknown bile acid species in rat bile which most likely classify as mono-oxo and di-oxo-taurocholates (MW 513 Da, 511 Da) and mono-oxo-glycocholates (MW 463 Da). In the present study we show that using this system it is possible to determine both conjugated and unconjugated as well as sulfated bile acids, without time consuming group separation and derivatization, from rat bile and human serum. In addition, it is suggested that the method presented here should be considered for use in routine analysis.

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Abbreviations

HPLC:

High Performance Liquid Chromatography

CFFAB:

Continuous Flow Fast Atom Bombardment

MS:

Mass Spectrometry

LC:

Liquid Chromatography

HPTLC:

High Performance Thin Layer Chromatography

GC:

glycocholate

TC:

taurocholate

GCDC:

glycochenodeoxycholate

TCDC:

taurochenodeoxycholate

GDC:

glycodeoxycholate

TDC:

taurodeoxycholate

GLC:

glycolithocholate

TLC:

taurolithocholate

GUDC:

glycoursodeoxycholate

TUDC:

tauroursodeoxycholate

GHC:

glycohyocholate

THC:

taurohyocholate

GHDC:

glycohyodeoxycholate

THDC:

taurohyodeoxycholate

α-GM:

α-glycomuricholate

α-TM:

α-tauromuricholate

β-GM:

β-glycomuricholate

β-TM:

β-tauromuricholate

ω-GM:

ω-glycomuricholate

ω-TM:

ω-tauromuricholate

References

  1. R. Colman, Biochem. Soc. Transactions15, 68 (1987).

    Google Scholar 

  2. I. M. Arias, W. B. Jacoby, H. Popper, D. Schachter, D. A. Shafritz, “The Liver in Biology and Pathobiology”, Raven Press, New York (1988).

    Google Scholar 

  3. R. Balducci, A. Roda, R. S. Pearlman, J. Solution Chem.18, 355 (1989).

    Article  CAS  Google Scholar 

  4. K. Miyasaka, A. Funakoshi, F. Shikado, K. Kitani, Gastroenterology102, 598 (1992).

    CAS  Google Scholar 

  5. P. H. E. Groot, N. J. Pearce, K. E. Suckling, S. Eisnberg, Biochim. Biophys. Acta1123, 76 (1992).

    CAS  Google Scholar 

  6. O. Hernell, J. E. Staggers, M. C. Carey, Biochemistry29, 2041 (1990).

    Article  CAS  Google Scholar 

  7. J. E. Staggers, O. Hernell, R. J. Stafford, M. C. Carey, Biochemistry29, 2028 (1990).

    Article  CAS  Google Scholar 

  8. M. C. Bateson, Brit. Med. J.284, 1 (1992).

    Google Scholar 

  9. J. M. Street, D. J. H. Trafford, H. L. J. Makin, J. Lipid Res.24, 491 (1983).

    CAS  Google Scholar 

  10. H. R. Almond, Z. R. Vlahcevic, C. C. Bell, D. H. Gregory, L. Swell, N. Engl. J. Med.289, 1213 (1973).

    CAS  Google Scholar 

  11. G. E. Muller, H. Bockhorn, R. Fleischmann, G. H. Muller, Transplant. Proc.13, 845 (1981).

    CAS  Google Scholar 

  12. D. Labbé, M. F. Gerhardt, A. Myara, C. Vercambre, F. Trivin, J. Chromatogr.490, 275 (1989).

    Google Scholar 

  13. K. Shimada, Y. Komine, K. Mitamura, J. Chromatogr.565, 111 (1991).

    CAS  Google Scholar 

  14. S. Yoshida, H. Murai, S. Hirose, T. Takeda, J. Chromatogr.431, 27 (1988).

    CAS  Google Scholar 

  15. J. Goto, Y. Teraya, T. Nambara, J. Chromatogr.585, 281 (1991).

    Article  CAS  Google Scholar 

  16. J. Goto, H. Murai, M. Inada, T. Nambara, T. Nagakura, H. Suzuki, J. Chromatogr.452, 119 (1988).

    Article  CAS  Google Scholar 

  17. C. Eckers, N. J. Haskins, T. Large, Biomed. Environ. Mass Spectrometry18, 702 (1989).

    CAS  Google Scholar 

  18. C. Eckers, P. B. East, N. J. Haskins, Biol. Mass Spectrometry20, 731 (1991).

    CAS  Google Scholar 

  19. K. D. R. Setchell, C. H. Vestal, J. Lipid Res.30, 1459 (1989).

    CAS  Google Scholar 

  20. B. L. Ackermann, J. Coutant, T.-M. Chen, in “Continuous Flow Fast Atom Bombardment Mass Spectrometry”, R. M. Caprioli, Ed., Wiley, Chichester (1990), p. 160.

    Google Scholar 

  21. J. E. Evans, A. Ghosh, B. A. Evans, M. R. Natowicz, Biol. Mass Spectrometry22, 331 (1993).

    CAS  Google Scholar 

  22. B. M. Warrack, G. C. DiDonnato, Biol. Mass. Spectrometry22, 101 (1993).

    CAS  Google Scholar 

  23. M. Kindel, H. Ludwig-Koehn, B. Lembcke, J. Chromatogr.497, 139 (1989).

    CAS  Google Scholar 

  24. L. Lepri, D. Heimler, P. G. Desideri, J. Chromatogr.288, 461 (1984).

    Article  CAS  Google Scholar 

  25. S. Müllner, R. Hofmann, K. Saar, B. Karbe-Thönges, J. Planar Chromatogr.5, 408 (1992).

    Google Scholar 

  26. A. Rivas-Nass, S. Müllner, J. Planar Chromatogr.7, 278 (1994).

    CAS  Google Scholar 

  27. R. E. Levitt, J. C. Touchstone, J. High Resolut. Chromatogr. Commun.2, 588 (1979).

    Google Scholar 

  28. K. L. Busch, J. Planar Chromatogr.2, 355 (1989).

    CAS  Google Scholar 

  29. K. D. R. Setchell, A. Matsui, Clin. Chim. Acta127, 1 (1983).

    Article  CAS  Google Scholar 

  30. K. D. R. Setchell, J. Worthington, Clin. Chim. Acta125, 135 (1982).

    Article  CAS  Google Scholar 

  31. J. M. Street, D. J. H. Trafford, H. L. J. Makin, J. Chromatogr.343, 259 (1985).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacy and Biological Chemistry, University of the Saarland, FR 12.5. Food Science, 66123, Saarbrücken, Germany

    C. Dax

  2. Corporate Research, Hoechst AG, 65926, Frankfurt/M., Germany

    M. Vogel

  3. Biomedical Research, Hoechst AG Werk-Kalle Albert, PO Box 3540, 65174, Wiesbaden, Germany

    S. Müllner

Authors
  1. C. Dax
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  2. M. Vogel
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  3. S. Müllner
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Dax, C., Vogel, M. & Müllner, S. HPLC-continuous-flow fast atom bombardment mass spectrometry (HPLC-CFFAB) — a convenient method for the analysis of bile acids in bile and serum. Chromatographia 40, 674–679 (1995). https://doi.org/10.1007/BF02315136

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