Summary
A chemically modified electrode was applied as a working electrode for the amperometric detection of sugars in liquid chromatography. A mixture of cuprous oxide and conductive carbon cement was used as electrode material. Cuprous oxide acted as a catalyst for the oxidation of sugars. Cyclic voltammetry was used to examine the oxidation of the sugars at the electrode surface. The stability and sensitivity of a cuprous oxide/conductive carbon cement (20/80 %, w/w) electrode were tested in flow injection analysis experiments. For liquid chromatography, sugars were separated on an ion-exclusion column with 0.01 M H2SO4 as mobile phase. After the separation, 0.2 M NaOH was added post-column and the sugars were determined at 550 mV vs. Ag/AgCl. Calibration was linear in the range of 10−6·10−3 M with limits of detection of approximately 1·10−6M. The application of the modified electrode for the determination of sugars in fruit beverages and dairy products following chromatographic separation is shown.
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References
J. A. M. van Riel, C. Olieman, Carbohydrate Research215, 39 (1991).
S. K. Sanghi, W. Th. Kok, G. C. M. Koomen, F. J. Hoek, Anal. Chim. Acta,273, 443 (1993).
D. C. Johnson, W. R. LaCourse, Anal. Chem.61, 589A (1990).
K. N. Thomsen, R. P. Baldwin, Anal. Chem.61, 2594 (1989).
J. M. Zadeii, J. Marioli, Anal. Chem.63, 649 (1991).
I. G. Casella, E. Desimoni, T. R. I. Cataldi, Anal. Chim. Acta248, 117 (1991).
I. G. Casella, E. Desimoni, A. M. Salvi, Anal. Chim. Acta243, 61 (1991).
P. Luo, F. Zhang, R. P. Baldwin, Anal. Chem.63, 1702 (1991).
T. R. I. Cataldi, I. G. Casella, E. Desimoni, Anal. Chim. Acta270, 161 (1992).
Z. Gao, A. Ivaska, Anal. Chim. Acta259, 211 (1992).
L. M. Santos, R. P. Baldwin, Anal. Chim. Acta206, 85 (1988).
J. Wang, Z. Taha, Anal. Chem.62, 1413 (1990).
Y. Xie, C. O. Huber, Anal. Chem.63, 1714 (1991).
X. Huang, W. Th. Kok, Anal. Chim. Acta273, 245 (1993).
X. Huang, J. J. Pot, W. Th. Kok, Anal. Chim. Acta,300, 5 (1995).
J. M. Elbicki, D. M. Morgan, S. G. Weber, Anal. Chem.56, 978 (1984).
J. G. Dawber, D. R. Brown, R. A. Reed, J. Chem. Educ.43, 34 (1966).
E. W. Reed, J. S. Dranoff, Ind. Eng. Chem. Fund.3, 304 (1964).
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Huang, X., Pot, J.J. & Kok, W.T. Determination of sugars by liquid chromatography and amperometric detection with a cuprous oxide modified electrode. Chromatographia 40, 684–689 (1995). https://doi.org/10.1007/BF02315138
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DOI: https://doi.org/10.1007/BF02315138