ALBERT

Description: Micro-Raman spectroscopy has been used on adult bivalve shells to investigate organic and inorganic shell components but has not yet been applied to bivalve larvae. It is known that the organic matrix of larval shells contains pigments, but less is known about the presence or source of these molecules in larvae. We investigated Raman spectra of seven species of bivalve larvae to assess the types of pigments present in shells of each species and how the ratio of inorganic : organic material changes in a dorso-ventral direction. In laboratory experiments, we reared larvae of three clam species in waters containing different organic signatures to determine if larvae incorporated compounds from source waters into their shells. We found differences in spectra and pigments between most species but found less intraspecific differences. A neural network classifier for Raman spectra classified five out of seven species with greater than 85% accuracy. There were slight differences between the amount and type of pigment present along the shell, with the prodissoconch I and shell margin areas being the most variable. Raman spectra of 1-day-old larvae were found to be differentiable when larvae were reared in waters with different organic signatures. With micro-Raman spectroscopy, it may be possible to identify some unknown species in the wild and trace their natal origins, which could enhance identification accuracy of bivalve larvae and ultimately aid management and restoration efforts.

Description: Tephra provides regional chronostratigraphical marker horizons that can link different climate archives with highly needed accuracy and precision. The results presented in this work exemplify, however, that the intermittent storage of tephra in ice sheets and during its subsequent iceberg transport, especially during glacial stages, constitutes a potential source of serious error for the application of tephrochronology to Nordic Seas and North Atlantic sediment archives. The peak shard concentration of the rhyolitic component of the North Atlantic Ash Zone II (NAAZ-II) tephra complex, often used to correlate marine and ice core records in Marine Isotope Stage (MIS) 3, is shown to lag the eruption event by ca. 100–400 years in some North Atlantic and Norwegian Sea cores. While still allowing for a correlation of archives on millennial timescales, this time delay in deposition is a major obstacle when addressing the lead–lag relationship on short timescales (years to centuries). A precise and accurate determination of lead–lag relationships between archives recording different parts of the climate system is crucial in order to test hypotheses about the processes leading to abrupt climate change and to evaluate results from climate models. Copyright # 2011 John Wiley & Sons, Ltd.

Description: Many of the difficulties of staining plastic embedded tissues for light and electron microscopy derive from physical exclusion of hydrophilic staining reagents by hydrophobic embedding media. Structures which stain most intensely with hydrophilic reagents usually contain less hydrophobic plastic than do non-staining structures. Such incomplete infiltration is apparently caused by exclusion of viscous, hydrophobic monomers by physically dense and/or well hydrated tissue elements. In keeping with this, generalized staining of tissues embedded in hydrophobic media does occur when hydrophobic reagents are used. Staining of plastic-free structures with single hydrophilic reagents or with sequences of such reagents, is, however, largely rate-controlled. The surprising similarity of hydrophilic and hydrophobic plastic embedding media is discussed. Limits of this simple model are explored, with a consideration of the roles of fixative and of monomer-tissue reactions

Description: INTRODUCTION: Lichens are self-sustaining partnerships comprising fungi as shape-forming partners for their enclosed symbiotic algae. They produce a tremendous diversity of metabolites (1050 metabolites described so far). OBJECTIVES: A comparison of metabolic profiles in nine lichen species belonging to three genera (Lichina, Collema and Roccella) by using an optimised extraction protocol, determination of the fragmentation pathway and the in situ localisation for major compounds in Roccella species. METHODS: Chemical analysis was performed using a complementary study combining a Taguchi experimental design with qualitative analysis by high-performance liquid chromatography coupled with mass spectrometry techniques. RESULTS: Optimal conditions to obtain the best total extraction yield were determined as follows: mortar grinding to a fine powder, two successive extractions, solid:liquid ratio (2:60) and 700 rpm stirring. Qualitative analysis of the metabolite profiling of these nine species extracted with the optimised method was corroborated using MS and MS/MS approaches. Nine main compounds were identified: 1 β-orcinol, 2 orsellinic acid, 3 putative choline sulphate, 4 roccellic acid, 5 montagnetol, 6 lecanoric acid, 7 erythrin, 8 lepraric acid and 9 acetylportentol, and several other compounds were reported. Identification was performed using the m/z ratio, fragmentation pathway and/or after isolation by NMR analysis. The variation of the metabolite profile in differently organised parts of two Roccella species suggests a specific role of major compounds in developmental stages of this symbiotic association. CONCLUSION: Metabolic profiles represent specific chemical species and depend on the extraction conditions, the kind of the photobiont partner and the in situ localisation of major compounds.

Description: In order to test the influences of ocean acidification on the ocean pelagic ecosystem, so far the largest CO2 manipulation mesocosm study (European Project on Ocean Acidification, EPOCA) was performed in Kings Bay (Kongsfjorden), Spitsbergen. During a 30 day incubation, bacterial diversity was investigated using DNA fingerprinting and clone library analysis of bacterioplankton samples. Terminal restriction fragment length polymorphism (T-RFLP) analysis of the PCR amplicons of the 16S rRNA genes revealed that general bacterial diversity, taxonomic richness and community structure were influenced by the variation of productivity during the time of incubation, but not the degree of ocean acidification. A BIOENV analysis suggested a complex control of bacterial community structure by various biological and chemical environmental parameters. The maximum apparent diversity of bacterioplankton (i.e., the number of T-RFs) in high and low pCO2 treatments differed significantly. A negative relationship between the relative abundance of Bacteroidetes and pCO2 levels was observed for samples at the end of the experiment by the combination of T-RFLP and clone library analysis. Our study suggests that ocean acidification affects the development of bacterial assemblages and potentially impacts the ecological function of the bacterioplankton in the marine ecosystem.

Description: The Atlantic meridional overturning circulation (AMOC) carries large amounts of heat into the North Atlantic influencing climate regionally as well as globally. Palaeo-records and simulations with comprehensive climate models suggest that the positive salt-advection feedback may yield a threshold behaviour of the system. That is to say that beyond a certain amount of freshwater flux into the North Atlantic, no meridional overturning circulation can be sustained. Concepts of monitoring the AMOC and identifying its vicinity to the threshold rely on the fact that the volume flux defining the AMOC will be reduced when approaching the threshold. Here we advance conceptual models that have been used in a paradigmatic way to understand the AMOC, by introducing a density-dependent parameterization for the Southern Ocean eddies. This additional degree of freedom uncovers a mechanism by which the AMOC can increase with additional freshwater flux into the North Atlantic, before it reaches the threshold and collapses: an AMOC that is mainly wind-driven will have a constant upwelling as long as the Southern Ocean winds do not change significantly. The downward transport of tracers occurs either in the northern sinking regions or through Southern Ocean eddies. If freshwater is transported, either atmospherically or via horizontal gyres, from the low to high latitudes, this would reduce the eddy transport and by continuity increase the northern sinking which defines the AMOC until a threshold is reached at which the AMOC cannot be sustained. If dominant in the real ocean this mechanism would have significant consequences for monitoring the AMOC.

Description: Uncertainty over the identity and age of Toba tephras across peninsular India persists, with radiometric age dates contradicting earlier compositional data, which have been used to identify this important Stratigraphie marker as the Youngest Toba Tuff (YTT). To address this issue, new single glass shard analyses have been performed for samples from Morgaon and Bori (north-western India), which have recently been dated at c. 800 ka. These, and indeed all Toba tephra samples thus far analysed from India, show the presence of four populations of glass shards (defined by their Ba/Y ratio), which uniquely identifies them as products of the c. 75-ka Youngest Toba eruption. Confirmation that the YTT fingerprint is characteristic comes from new analyses of Oldest Toba Tuff (OTT) glass shards from five sites in the Indian Ocean. These are compositionally identical to Layer D from the ODP site 758 Sediment core (c. 800 ka), and belong to a single, low-Ba population, clearly different from YTT. These analyses show that there is essentially no reworked OTT material in the YTT eruption, and indicate unequivocally that all known Toba tephra occurrences in India belong to the c. 75-ka Youngest Toba eruption.

Description: Fucoidan, a sulfated polysaccharide in brown seaweed, has various biological activities including anti-tumor activity. We investigated the effects of fucoidan on the apoptosis of human promyeloid leukemic cells and fucoidan-mediated signaling pathways. Fucoidan induced apoptosis of HL-60, NB4, and THP-1 cells, but not K562 cells. Fucoidan treatment of HL-60 cells induced activation of caspases-8, -9, and -3, the cleavage of Bid, and changed mitochondrial membrane permeability. Fucoidan-induced apoptosis, cleavage of procaspases, and changes in the mitochondrial membrane permeability were efficiently blocked by depletion of mitogen-activated protein kinase (MAPK) kinase kinase 1 (MEKK1), and inhibitors of MAPK kinase 1 (MEK1) and c Jun NH2-terminal kinase (JNK). The phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and JNK was increased in fucoidan-treated HL-60, NB4, and THP-1 cells, but not K562 cells. ERK1/2 activation occurred at earlier times than JNK activation and JNK activation was blocked by MEK1 inhibitor. In addition, fucoidan-induced apoptosis was inhibited by addition of glutathione and/or L-NAME, and fucoidan decreased intracellular glutathione concentrations and stimulated nitric oxide (NO) production. Buthionine-[R,S]-sulfoximine rendered HL-60 cells more sensitive to fucoidan. Depletion of MEKK1 and inhibition of MEK1 restored the intracellular glutathione content and abrogated NO production, whereas inhibition of JNK activation by SP600125 restored intracellular glutathione content but failed to inhibit NO production in fucoidan-treated HL-60 cells. These results suggest that activation of MEKK1, MEK1, ERK1/2, and JNK, depletion of glutathione, and production of NO are important mediators in fucoidan-induced apoptosis of human leukemic cells

Description: Axial volcanic ridges (AVRs) are found on most slow-spreading mid-ocean ridges and are thought to be the main locus of volcanism there. In this study we present high-resolution mapping of a typical, well-defined AVR on the Mid-Atlantic Ridge at 45°N. The AVR is characterized by “hummocky terrain,” composed typically of hummocks with pillowed or elongate pillowed flanks with pillowed or lobate lava flow summits, often with small haystacks sitting on their highest points. The AVR is surrounded by several areas of “flat seafloor,” composed of lobate and sheet lava flows. The spatial and morphological differences between these areas indicate different eruption processes operating on and off the AVR. Volcanic fissures are found all around and on the AVR, although those with the greatest horizontal displacement are found on the ridge crest and flat seafloor. Clusters of fissures may represent volcanic vents. Extremely detailed comparisons of sediment coverage and examination of contact relations around the AVR suggest that many of the areas of flat seafloor are of a similar age or younger than the hummocky terrain of the AVR. Additionally, all the lavas surveyed have similar degrees of sediment cover, suggesting that the AVR was either built or resurfaced in the same 50 ka time frame as the flat seafloor.

Description: Forazoline A, a novel antifungal polyketide with in vivo efficacy against Candida albicans, was discovered using LCMS-based metabolomics to investigate marine-invertebrate-associated bacteria. Forazoline A had a highly unusual and unprecedented skeleton. Acquisition of 13C–13C gCOSY and 13C–15N HMQC NMR data provided the direct carbon–carbon and carbon–nitrogen connectivity, respectively. This approach represents the first example of determining direct 13C–15N connectivity for a natural product. Using yeast chemical genomics, we propose that forazoline A operated through a new mechanism of action with a phenotypic outcome of disrupting membrane integrity.

Description: Direct estimations of turbulent fluxes and atmospheric stability were performed from a sonic anemometer at 50 m height on a meteorological mast at the Horns Rev wind farm in the North Sea. The stability and flux estimations from the sonic measurements are compared with bulk results from a cup anemometer at 15 m height and potential temperature differences between the water and the air above. Surface flux estimations from the advanced weather research and forecast (WRF) model are also validated against the sonic and bulk data. The correlation between the sonic and bulk estimates of friction velocity is high and the highest among all velocity comparisons. From the sonic–bulk–WRF inter-comparison, it is found that the atmospheric stability measures at the sonic height tend to be closer to the neutral value than the WRF and bulk estimates, which are performed within an air layer closer to the surface, not only from a systematic bulk and WRF under-prediction of the friction velocity when compared with the sonic value but also because of the lower magnitude of the sonic heat flux compared with that from the WRF simulations. Although they are not measured but parameterized or estimated, the bulk–WRF comparisons of friction velocity and 10 m wind speed show good agreement. It is also shown that on a long-term basis, the WRF and bulk estimates of stability are nearly equal and that a correction towards a slightly stable atmospheric condition has to be applied to the long-term wind profile at Horns Rev and at other locations over the North Sea, the correction being larger for points close to the coast.

Description: Mutations or environmental factors that result in reversal of conspicuous left–right asymmetries provide an opportunity to study developmental mechanisms. They may also provide insight into evolutionary changes in asymmetry states within and between species. King crabs (family Lithodidae) have a larger right claw and females typically exhibit a dextrally offset abdomen. Nevertheless, I observed a high incidence of left handedness in laboratory reared box crabs (Lopholithodes foraminatus) and captured the first known egg-bearing female lithodid to exhibit reversed asymmetry. This provided a unique opportunity to characterize the reversed phenotype and to compare the incidence of reversed asymmetry in the offspring of normal and reversed females. Asymmetry of the chelae became apparent in the first postzoeal stage (glaucothoe) and handedness was maintained through subsequent instars. Females with larger left claws developed reversed abdominal asymmetry by the fourth crab stage. No reversed asymmetry was observed in the mandibles of zoea larvae or juveniles of either handedness. The incidence of reversed asymmetry in glaucothoe reared from one reversed and three normal females was high (between 20% and 30%), and independent of maternity (P=0.67). Removal of the right cheliped of fourth stage zoeae, and the major cheliped of glaucothoe, did not reverse the direction of asymmetry. Elevated larval rearing temperature also did not affect the frequency of reversed individuals. This lack of evidence for either heritability or induction of handedness is enigmatic. Further investigation of reversed asymmetry in lithodid crabs may provide valuable insights into the development and evolution of bilateral asymmetries.

Description: Here we show the use of the 210Pb-226Ra excess method to determine the growth rate of two corals from the world's largest known cold-water coral reef, Røst Reef, north of the Arctic circle off Norway. Colonies of each of the two species that build the reef, Lophelia pertusa and Madrepora oculata, were collected alive at 350 m depth using a submersible. Pb and Ra isotopes were measured along the major growth axis of both specimens using low level alpha and gamma spectrometry and trace element compositions were studied. 210Pb and 226Ra differ in the way they are incorporated into coral skeletons. Hence, to assess growth rates, we considered the exponential decrease of initially incorporated 210Pb, as well as the increase in 210Pb from the decay of 226Ra and contamination with 210Pb associated with Mn-Fe coatings that we were unable to remove completely from the oldest parts of the skeletons. 226Ra activity was similar in both coral species, so, assuming constant uptake of 210Pb through time, we used the 210Pb-226Ra chronology to calculate growth rates. The 45.5 cm long branch of M. oculata was 31 yr with an average linear growth rate of 14.4 ± 1.1 mm yr−1 (2.6 polyps per year). Despite cleaning, a correction for Mn-Fe oxide contamination was required for the oldest part of the colony; this correction corroborated our radiocarbon date of 40 yr and a mean growth rate of 2 polyps yr−1. This rate is similar to the one obtained in aquarium experiments under optimal growth conditions.

Description: Eutrophication, coupled with loss of herbivory due to habitat degradation and overharvesting, has increased the frequency and severity of macroalgal blooms worldwide. Macroalgal blooms interfere with human activities in coastal areas, and sometimes necessitate costly algal removal programmes. They also have many detrimental effects on marine and estuarine ecosystems, including induction of hypoxia, release of toxic hydrogen sulphide into the sediments and atmosphere, and the loss of ecologically and economically important species. However, macroalgal blooms can also increase habitat complexity, provide organisms with food and shelter, and reduce other problems associated with eutrophication. These contrasting effects make their overall ecological impacts unclear. We conducted a systematic review and meta-analysis to estimate the overall effects of macroalgal blooms on several key measures of ecosystem structure and functioning in marine ecosystems. We also evaluated some of the ecological and methodological factors that might explain the highly variable effects observed in different studies. Averaged across all studies, macroalgal blooms had negative effects on the abundance and species richness of marine organisms, but blooms by different algal taxa had different consequences, ranging from strong negative to strong positive effects. Blooms' effects on species richness also depended on the habitat where they occurred, with the strongest negative effects seen in sandy or muddy subtidal habitats and in the rocky intertidal. Invertebrate communities also appeared to be particularly sensitive to blooms, suffering reductions in their abundance, species richness, and diversity. The total net primary productivity, gross primary productivity, and respiration of benthic ecosystems were higher during macroalgal blooms, but blooms had negative effects on the productivity and respiration of other organisms. These results suggest that, in addition to their direct social and economic costs, macroalgal blooms have ecological effects that may alter their capacity to deliver important ecosystem services.

Description: Transfer of the small gaseous molecules CO2 and NH3 across biological membranes, long thought to occur solely by simple diffusion, is now known to be facilitated by members of two multigene families: aquaporins (AQP) and rhesus (Rh) proteins. Although it is accepted that AQP1 and Rh proteins are involved in CO2 and NH3 transfer, respectively, the idea that a single channel can exhibit selectivity for both gases is controversial. Indeed, studies using the same in vitro model (human red blood cells) have provided evidence both for and against a role for Rh proteins as CO2 channels. Thus, this study was initiated to provide in vivo evidence for a dual function of Rh proteins as ammonia and CO2 channels. Here, we show that in zebrafish (Danio rerio), direct ammonia–CO2 competition experiments in adults or translational knockdowns of Rh proteins in larvae affects both ammonia and CO2 excretion. These results suggest that Rh proteins in zebrafish may be common pathways for transport of ammonia and CO2.

Description: Metagenomic techniques are used to analyse bacterial communities allowing both culturable and unculturable species to be represented. However, the screening of oral metagenomic samples can be hindered by high animal host DNA content. This study evaluated methods for the reduction of human DNA concentrations within oral metagenomic samples. Plaque samples were collected from 27 patients presenting with periodontal disease and treated to remove human DNA using either selective lysis of eukaryotic cells at several buffer concentrations or differential centrifugation after treatment with trypsin and/or detergents. Human and bacterial DNA levels were determined by quantitative polymerase chain reaction (qPCR). The human DNA content of plaque extracts was significantly reduced by all treatments compared with an untreated control (P 〈 0.05). However, differential centrifugation simultaneously reduced the bacterial DNA content unless samples were pretreated with a detergent. Observations of Gram stained samples that were processed using differential centrifugation without detergent suggest that many bacteria remain adhered to human cells. An approach that uses differential centrifugation in parallel with selective lysis is recommended to fully represent the oral microbiota in metagenomic samples, including those tightly adhered to human cells and more delicate bacteria such as Mycoplasma.

Description: The evolution of internal structure plays a pivotal role in the macroscopic response of granular materials to applied loads. A case in point is the so‐called ‘stress–dilatancy relation’, a cornerstone of Soil Mechanics. Numerous attempts have been made to unravel the connection between stress–dilatancy and the evolution of fabric and contact forces in a deforming granular medium. We re‐examine this connection in light of the recent findings on force chain evolution, in particular, that of collective force chain failure by buckling. This study is focussed on two‐dimensional deformation of dense granular assemblies. Analysis of individual and collective force chain bucklings is undertaken using data from a discrete element simulation. It is shown that the kinematics of force chain buckling lead to significant levels of local dilatation being developed in the buckling force chain particles and their confining first‐ring neighbors. Findings from the simulation are used to guide the development of a lattice model of collective, localized force chain buckling. Consideration of the statics and kinematics of this process yields a new stress–dilatancy relation. The physics of buckling, even at its simplest form, introduces a richness into the stress–dilatancy formulation in a way that preserves the essential aspects of fabric evolution, specifically the buckling mode.

Description: Marine myxobacteria (Enhygromyxa, Plesiocystis, Pseudoenhygromyxa, Haliangium) are phylogenetically distant from their terrestrial counterparts. Salimabromide is the first natural product from the Plesiocystis/Enhygromyxa clade of obligatory marine myxobacteria. Salimabromide has a new tetracyclic carbon skeleton, comprising a brominated benzene ring, a furano lactone residue, and a cyclohexane ring, bridged by a seven-membered cyclic moiety. The absolute configuration was deduced from experimental and calculated CD data. Salimabromide revealed antibiotic activity towards Arthrobacter cristallopoietes.

Description: Cold-water coral (CWC) reefs are heterogeneous ecosystems comprising numerous microhabitats. A typical European CWC reef provides various biogenic microhabitats (within, on and surrounding colonies of coral species such as Lophelia pertusa, Paragorgia arborea and Primnoa resedaeformis, or formed by their remains after death). These microhabitats may be surrounded and intermixed with non-biogenic microhabitats (soft sediment, hard ground, gravel/pebbles, steep walls). To date, studies of distribution of sessile fauna across CWC reefs have been more numerous than those investigating mobile fauna distribution. In this study we quantified shrimp densities associated with key CWC microhabitat categories at the Røst Reef, Norway, by analysing image data collected by towed video sled in June 2007. We also investigated shrimp distribution patterns on the local scale (〈40 cm) and how these may vary with microhabitat. Shrimp abundances at the Røst Reef were on average an order of magnitude greater in biogenic reef microhabitats than in non-biogenic microhabitats. Greatest shrimp densities were observed in association with live Paragorgia arborea microhabitat (43 shrimp m−2, SD = 35.5), live Primnoa resedaeformis microhabitat (41.6 shrimp m−2, SD = 26.1) and live Lophelia pertusa microhabitat (24.4 shrimp m−2, SD = 18.6). In non-biogenic microhabitat, shrimp densities were 〈2 shrimp m−2. CWC reef microhabitats appear to support greater shrimp densities than the surrounding non-biogenic microhabitats at the Røst Reef, at least at the time of survey.

Description: Submarine canyons are known as one of the seafloor morphological features where living cold-water coral (CWC) communities develop in the Mediterranean Sea. We investigated the CWC community of the two westernmost submarine canyons of the Gulf of Lions canyon system: the Cap de Creus Canyon (CCC) and Lacaze-Duthiers Canyon (LDC). Coral associations have been studied through video material recorded by means of a manned submersible and a remotely operated vehicle. Video transects have been conducted and analyzed in order to obtain information on (1) coral bathymetric distribution and density patterns, (2) size structure of coral populations, and (3) coral colony position with respect to the substrate. Madrepora oculata was the most abundant CWC in both canyons, while Lophelia pertusa and Dendrophyllia cornigera mostly occurred as isolated colonies or in small patches. An important exception was detected in a vertical cliff in LDC where a large L. pertusa framework was documented. This is the first record of such an extended L. pertusa framework in the Mediterranean Sea. In both canyons coral populations were dominated by medium and large colonies, but the frequent presence of small-sized colonies also indicate active recruitment. The predominant coral orientation (90° and 135°) is probably driven by the current regime as well as by the sediment load transported by the current flows. In general, no clear differences were observed in the abundance and in the size structure of the CWC populations between CCC and LDC, despite large differences in particulate matter between canyons.

Description: Cormorants, Phalacocorax spp., have great potential to affect recreational fisheries and have increased substantially in abundance over the last decades in UK inland waters. Fabricated refuges provide a potential means of reducing fish losses, yet the benefits of such structures may be marginal if natural shelters are abundant and favoured, or if strong density-dependence limits refuge use. This study examined the efficacy of artificial shelters in mesocosm enclosures that allowed standardised and replicated observations of roach, Rutilus rutilus (L.), distribution. When given a choice between occupying open water, simulated reedbeds and artificial brushwood shelters, roach used brushwood shelters extensively across a range of fish densities. When fish had a choice of occupying open water or reedbeds offering no overhead cover, they actively avoided reedbeds and used open water almost exclusively. Occupation of reedbeds was positively related to the amount of overhead cover they provided. When artificial brushwood shelters and reedbeds offered complete overhead cover, brushwood shelters were occupied twice as much as reedbeds. Artificial shelters may therefore have wide application in stillwaters with abundant reedbeds unless the reedbeds are coupled with extensive overhead cover.

Description: Methane is a potent greenhouse gas and large-scale rapid release of methane from hydrate may have contributed to past abrupt climate change inferred from the geological record. The discovery in 2008 of over 250 plumes of methane gas escaping from the seabed of the West Svalbard continental margin at ~400 m water depth (mwd) suggests that hydrate is dissociating in the present-day Arctic. Here we model the dynamic response of hydrate-bearing sediments over a period of 2300 years and investigate ocean warming as a possible cause for present-day and likely future dissociation of hydrate, within 350–800 mwd, west of Svalbard. Future temperatures are given by two climate models, HadGEM2 and CCSM4, and scenarios, Representative Concentration Pathways (RCPs) 8.5 and 2.6. Our results suggest that over the next three centuries 5.3–29 Gg yr−1 of methane may be released to the Arctic Ocean on the West Svalbard margin.

Description: This article compares the quality of Raman images obtained using metallurgical and oil immersion objectives to map complex structures in two and three dimensions. While the performance of these objectives for depth profiling planar structures has been discussed at length in the literature, the same comparison has not yet been made for 3D mapping of complex objects, where additional complications are introduced by non-planar geometries. Studying samples with increasing complexity shows that the oil immersion objective is strongly preferred because it yields brighter images with better contrast, and eliminates some new and confusing artefacts that do not arise with simple planar objects.

Description: Although it has been more than 30 years since the discovery of deep-sea hydrothermal vents, comprehending the interconnections between hydrothermal venting and microbial life remains a challenge. Here we investigate abiotic-biotic linkages in low-temperature hydrothermal biotopes at Desperate and Lilliput on the southern Mid-Atlantic Ridge. Both sites are basalt-hosted and fluids exhibit the expected chemical signatures. However, contrasting crustal permeabilities have been proposed, supporting pervasive mixing at Desperate but restricting circulation at Lilliput. In Desperate fluids, sulfide and O2 were readily available but H2 hardly detectable. Under incubation conditions (oxic unamended, sulfide-spiked, oxic and anoxic H2 -spiked at 18°C), only sulfide oxidation by Thiomicrospira fuelled biomass synthesis. Microbial phylogenies from Desperate incubation experiments resembled those of the natural samples suggesting that the incubation conditions mimicked the environment. In Lilliput fluids, O2 was limited, whereas sulfide and H2 were enriched. Autotrophy appeared to be stimulated by residual sulfide and by amended H2 . Yet, based on bacterial phylogenies only conditions in anoxic H2 -spiked Lilliput incubations appeared similar to parts of the Lilliput habitat. In anoxic H2 -spiked Lilliput enrichments Campylobacteraceae likely supported biomass production through H2 oxidation. We argue that the diverging circulation patterns arising from different subseafloor permeabilities act as major driving forces shaping these biotope structures.

Description: 1. The hypothesis that cyanobacteria have higher optimum growth temperatures and higher growth rates at the optimum as compared to chlorophytes was tested by running a controlled experiment with eight cyanobacteria species and eight chlorophyte species at six different temperatures (20–35 °C) and by performing a literature survey. 2. In the experiment, all organisms except the chlorophyte Monoraphidium minutum grew well up to 35 °C. The chlorophyte Chlamydomonas reinhardtii was the fastest-growing organism over the entire temperature range (20–35 °C). 3. Mean optimum growth temperatures were similar for cyanobacteria (29.2 °C) and chlorophytes (29.2 °C). These results are concordant with published data, yielding slightly higher mean optimum growth temperatures for cyanobacteria (27.2 °C) than for chlorophytes (26.3 °C). 4. Mean growth rates of cyanobacteria at 20 °C (0.42 day−1) were significantly lower than those of chlorophytes at 20 °C (0.62 day−1). However, at all other temperatures, there were no differences between mean growth rates of cyanobacteria and chlorophytes. 5. Mean growth rates at the optimum temperature were similar for cyanobacteria (0.92 day−1) and chlorophytes (0.96 day−1). However, analysis of published data revealed that growth rates of cyanobacteria (0.65 day−1) were significantly lower than those of chlorophytes (0.93 day−1) at their optimum temperatures. 6. Although climate warming will probably lead to an intensification of cyanobacterial blooms, our results indicate that this might not be as a result of higher growth rates of cyanobacteria compared with their chlorophyte competitors. The competitive advantage of cyanobacteria can more likely be attributed to their ability to migrate vertically and prevent sedimentation in warmer and more strongly stratified waters and to their resistance to grazing, especially when warming reduces zooplankton body size.

Description: The Swedish UNESCO World Cultural Heritage site of the Birka and Hovgården Iron Age settlements is well suited for the testing of high-resolution archaeological prospection methods. In May 2006 ground-penetrating radar (GPR) and magnetometer test measurements were conducted at Birka, resulting in data of outstanding quality and new archaeological discoveries, but also demonstrating the need for increased spatial sampling regarding GPR prospection at complex Scandinavian sites. Therefore Birka was selected as a testing ground for a pilot study investigating the suitability of the novel multichannel GPR array system MIRA (MALÅ Imaging Radar Array) for efficient, large-scale GPR surveys with very dense spatial sampling. The study was conducted in May 2008 by MALÅ Geoscience AB in collaboration with the archaeological prospection unit of the Swedish National Heritage Board. The very high-resolution three-dimensional GPR pilot survey demonstrated that it is possible to survey 1 ha and more per day with 8 cm cross-line spacing, mapping archaeological structures in unprecedented resolution, such as postholes of only 25 cm diameter. This paper describes the tested technology and methodology as well as the fieldwork and the results of the study.

Description: In many geotechnical systems, it is not uncommon to observe failure in zones of high localized strain called shear bands. The existing models predict the existence and the extent of these localizations, but provide little insight into the micromechanics within the shear bands. This research captures and compares the variation in microstructure both inside and outside of shear bands that formed in physical laboratory plane strain and companion numerical two-dimensional discrete element method (DEM) biaxial compression experiments. Unsheared and sheared laboratory specimens of Ottawa 20–30 sand of varying dilatancy were solidified using a two-stage resin impregnation procedure. The solidified specimens were sectioned and the resulting surfaces were prepared for microstructure observation using optical bright-field microscopy and stereological analysis. Statistical properties of microstructural parameters for sub-regions in a grid pattern and along predefined inclined zones were determined. Similar measurements were performed on 2D DEM simulation specimens at varying strain levels to characterize the evolution of microstructure with increasing strain. The results showed how differences evolved in the mean, standard deviation, and entropy of void distributions with increasing global strain levels. The results indicate how disorder increases and that the material within the shear band does not adhere to the classical concept of critical state, but reaches a terminal void ratio that is largely a function of initial void ratio. Furthermore, there appears to be a transition zone between the far field and the fully formed shear block, as opposed to an abrupt delineation as is traditionally inferred.

Description: Based on quantitative X-ray diffraction mineralogical analysis of cores HU2008029-0006/0008 (857-m water depth) located south of the Davis Strait sill, we seek answers to two questions: (i) the temporal correlation of NW Labrador Shelf Sea Heinrich (H-) and Baffin Bay detrital carbonate (BBDC) events, and (ii) the provenance of the sediments? A sediment-unmixing model (SedUnMix) is used to determine provenance variations. Although 12 radiocarbon dates were obtained there were many age reversals and we favour a depth/age model for HU2008029-0008PC based on correlations to well-dated neighbouring cores north and south of Davis Strait. We find no correlation between H- and BBDC events except for H-0, which appears to be a broadly synchronous event along the NE Canadian margin and is correlative with an episode of major moraine building along this flank of the Laurentide Ice Sheet. Unmixing of the sediment composition based on six potential glacial source regions indicates that the bulk of the sediment in 0008PC is derived from glacial erosion of West Greenland, but is punctuated by intervals when dolomite-rich sediments, derived from outcrops north of Baffin Bay, dominate the composition. Estimated dates on these BBDC events are -13, 25 and 30–38 cal ka BP.

Description: Core HU97048-007PC was recovered from the continental Labrador Sea slope at a water depth of 945 m, 250km seaward from the mouth of Cumberland Sound, and 400km north of Hudson Strait. Cumberland Sound is a structural trough partly floored by Cretaceous mudstones and Paleozoic carbonates. The record extends from 10 to 58 ka. On-board logging revealed a complex series of lithofacies, including buff-colored detrital carbonate-rich sediments [Heinrich (H)-events] frequently bracketed by black facies. We investigate the provenance of these facies using quantitative X-ray diffraction on drill-core samples from Paleozoic and Cretaceous bedrock from the SE Baffin Island Shelf, and on the〈2-mm sediment fraction in a transect of five cores from Cumberland Sound to the NW Labrador Sea. A sediment unmixing program was used to discriminate between sediment sources, which included dolomite-rich sediments from Baffin Bay, calcite-rich sediments from Hudson Strait and discrete sources from Cumberland Sound. Results indicated that the bulk of the sediment was derived from Cumberland Sound, but Baffin Bay contributed to sediments coeval with H-0 (Younger Dryas), whereas Hudson Strait was the source during H-events 1–4. Contributions from the Cretaceous outcrops within Cumberland Sound bracket H-events, thus both leading and lagging Hudson Strait-sourced H-events.

Description: In this study we present a comparative quantification of CaCO3 production rates by rhodolith-forming coralline red algal communities situated in high polar latitudes and assess which environmental parameters control these production rates. The present rhodoliths act as ecosystem engineers, and their carbonate skeletons provide an important ecological niche to a variety of benthic organisms. The settings are distributed along the coasts of the Svalbard archipelago, being Floskjeret (78◦180N) in Isfjorden, Krossfjorden (79◦080N) at the eastern coast of Haakon VII Land, Mosselbukta (79◦530N) at the eastern coast of Mosselhalvøya, and Nordkappbukta (80◦310N) at the northern coast of Nordaustlandet. All sites feature Arctic climate and strong seasonality.

Description: In virtually every assessment of responses to large-scale crises and disasters, coordination is identified as a critical failure factor. After the crisis, official committees and political opponents often characterize the early phases of the response as a ‘failure to coordinate.’ Not surprisingly, improved coordination quickly emerges as the prescribed solution. Coordination, then, is apparently both the problem and the solution. But the proposed solutions rarely solve the problem: coordination continues to mar most crises and disasters. In the absence of a shared body of knowledge on coordination, it is hard to formulate a normative framework that allows for systematic assessment of coordination in times of crisis. As coordination is widely perceived as an important function of crisis and disaster management, this absence undermines a fair and balanced assessment of crisis management performance. This paper seeks to address that void. We aim to develop a framework that explains both the failure and success of crisis coordination. We do this by exploring the relevant literature, reformulating what coordination is and distilling from research the factors that cause failure and success.

Description: The purine alkaloid caffeine is a major component of many beverages such as coffee and tea. Caffeine and its metabolites theobromine and xanthine have been shown to have antioxidant properties. Caffeine can also act as adenosine-receptor antagonist. Although it has been shown that adenosine and antioxidants promote wound healing, the effect of caffeine on wound healing is currently unknown. To investigate the effects of caffeine on processes involved in epithelialisation, we used primary human keratinocytes, HaCaT cell line and ex vivo model of human skin. First, we tested the effects of caffeine on cell proliferation, differentiation, adhesion and migration, processes essential for normal wound epithelialisation and closure. We used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) proliferation assay to test the effects of seven different caffeine doses ranging from 0·1 to 5 mM. We found that caffeine restricted cell proliferation of keratinocytes in a dose-dependent manner. Furthermore, scratch wound assays performed on keratinocyte monolayers indicated dose-dependent delays in cell migration. Interestingly, adhesion and differentiation remained unaffected in monolayer cultures treated with various doses of caffeine. Using a human ex vivo wound healing model, we tested topical application of caffeine and found that it impedes epithelialisation, confirming in vitro data. We conclude that caffeine, which is known to have antioxidant properties, impedes keratinocyte proliferation and migration, suggesting that it may have an inhibitory effect on wound healing and epithelialisation. Therefore, our findings are more in support of a role for caffeine as adenosine-receptor antagonist that would negate the effect of adenosine in promoting wound healing.

Description: Geoengineering, especially its potentially fast and high-leverage versions, is often justified as a necessary response to possible future climate emergencies. In this article, we take the notion of ‘necessity’ in international law as a starting point in assessing how rapid, high-leverage geoengineering might be justified legally. The need to specify reliably ‘grave and imminent peril’ makes such a justification difficult because our scientific ability to predict abrupt climate change, for example, as tipping elements, is limited. The time it takes to establish scientific consensus as well as policy acceptance restricts the scope for effective forewarning and so pre-emptive justifications for geoengineering become more tempting. While recognizing that dangerous, large-scale impacts of climate change is becoming increasingly difficult to avoid, the pre-emptive, emergency frame is problematic. We suggest that arguments from emergency operate on a high level of uncertainty and tend toward hubristic attempts to shape the future, as well as tending to close down rather than open up space for deliberation. We conclude that the emergency frame is not likely to go away, that ignoring or repressing it is a dangerous response, and that more effort is required to defuse and disarm emergency rhetoric.

Description: In May 2006 high-resolution measurements using ground-penetrating radar (GPR) and magnetic gradiometer systems conducted over selected areas at the site of the Viking Age settlement and trading place Birka in central Sweden demonstrated the suitability of these methods for archaeological prospection of Scandinavian proto-urban settlements. The non-invasive geophysical surveys revealed numerous structural details of the settlement: houses, property boundaries, track-ways, buried remains of the town ramparts dating from different building periods, including a gate, were mapped with a manually operated single-channel GPR system and a four-channel magnetometer array. The combination of these two prospection methods, state-of-the-art data processing and visualization and archaeological interpretation within a geographical information system resulted in valuable new information about the UNESCOWorld Cultural Heritage Site Birka-Hovgården.We present methodology and results of this first archaeological prospection case study conducted in 2006.

Description: The eastern Mediterranean transient (EMT) was caused by a combination of high‐salinity waters intruding into the Aegean Sea and the two particularly strong winters of 1991–1992 and 1992–1993. The approach in this chapter is to search for specific signatures in the historic hydrographic observations, which date back to 1910. To deal with the problem that up into the 1950s the data not only are of limited precision but also have gaps of about 20 years, it is advantageous to consider the fact that the evolution of the actual EMT is rather well documented over a similar time span. The chapter begins by outlining the characteristics of the current EMT. Thereafter, a selection of suitable hydrographic observations among the available historic data is provided to compare these with signatures expected from the evolution of the actual EMT.

Description: This article presents a fundamental study on the role of particle breakage on the shear behavior of granular soils using the three-dimensional (3-D) discrete element method. The effects of particle breakage on the stress ratio, volumetric strain, plastic deformation, and shear failure behavior of dense crushable specimens undergoing plane strain shearing conditions are thoroughly investigated through a variety of micromechanical analyses and mechanism demonstrations. The simulation of a granular specimen is based on the effective modeling of realistic fracture behavior of single soil particles, which is demonstrated by the qualitative agreement between the results from platen compression simulations and those from physical laboratory tests. The simulation results show that the major effects of particle breakage include the reduction of volumetric dilation and peak stress ratio and more importantly the plastic deformation mechanisms and the shear failure modes vary as a function of soil crushability. Consistent macro- and micromechanical evidence demonstrates that shear banding and massive volumetric contraction depict the two end failure modes of a dense specimen, which is dominated by particle rearrangement–induced dilation and particle crushing–induced compression, respectively, with a more general case being the combination and competition of the two failure modes in the medium range of soil crushability and confining stress. However, it is further shown that a highly crushable specimen will eventually develop a shear band at a large strain because of the continuous decay of particle breakage.

Description: Zonal wind anomalies in the western equatorial Atlantic during late boreal winter to early summer precondition boreal summer cold/warm events in the eastern equatorial Atlantic (EEA) that manifest in a strong interannual Atlantic cold tongue (ACT) variability. Local intraseasonal wind fluctuations, linked to the St. Helena anticyclone, contribute to the variability of cold tongue onset and strength, particularly during years with preconditioned shallow thermoclines. The impact of cold tongue sea surface temperature (SST) anomalies on the wind field in the Gulf of Guinea is assessed. It contributes to the northward migration of humidity and convection and possibly the West African monsoon (WAM) jump. Copyright @ 2010 Royal Meteorological Society

Description: The Mediterranean Sea is a semi-enclosed sea characterized by high salinities, temperatures and densities. The net evaporation exceeds the precipitation, driving an anti-estuarine circulation through the Strait of Gibraltar, contributing to very low nutrient concentrations. The Mediterranean Sea has an active overturning circulation, one shallow cell that communicates directly with the Atlantic Ocean, and two deep overturning cells, one in each of the two main basins. It is surrounded by populated areas and is thus sensitive to anthropogenic forcing. Several dramatic changes in the oceanographic and biogeochemical conditions have been observed during the past several decades, emphasizing the need to better monitor and understand the changing conditions and their drivers. During 2011 three oceanographic cruises were conducted in a coordinated fashion in order to produce baseline data of important physical and biogeochemical parameters that can be compared to historic data and be used as reference for future observational campaigns. In this article we provide information on the Mediterranean Sea oceanographic situation, and present a short review that will serve as background information for the special issue in Ocean Science on "Physical, chemical and biological oceanography of the Mediterranean Sea". An important contribution of this article is the set of figures showing the large-scale distributions of physical and chemical properties along the full length

Description: Recent studies on the impacts of ocean acidification on pelagic communities have identified changes in carbon to nutrient dynamics with related shifts in elemental stoichiometry. In principle, mesocosm experiments provide the opportunity of determining temporal dynamics of all relevant carbon and nutrient pools and, thus, calculating elemental budgets. In practice, attempts to budget mesocosm enclosures are often hampered by uncertainties in some of the measured pools and fluxes, in particular due to uncertainties in constraining air–sea gas exchange, particle sinking, and wall growth. In an Arctic mesocosm study on ocean acidification applying KOSMOS (Kiel Off-Shore Mesocosms for future Ocean Simulation), all relevant element pools and fluxes of carbon, nitrogen and phosphorus were measured, using an improved experimental design intended to narrow down the mentioned uncertainties. Water-column concentrations of particulate and dissolved organic and inorganic matter were determined daily. New approaches for quantitative estimates of material sinking to the bottom of the mesocosms and gas exchange in 48 h temporal resolution as well as estimates of wall growth were developed to close the gaps in element budgets. However, losses elements from the budgets into a sum of insufficiently determined pools were detected, and are principally unavoidable in mesocosm investigation. The comparison of variability patterns of all single measured datasets revealed analytic precision to be the main issue in determination of budgets. Uncertainties in dissolved organic carbon (DOC), nitrogen (DON) and particulate organic phosphorus (POP) were much higher than the summed error in determination of the same elements in all other pools. With estimates provided for all other major elemental pools, mass balance calculations could be used to infer the temporal development of DOC, DON and POP pools. Future elevated pCO2 was found to enhance net autotrophic community carbon uptake in two of the three experimental phases but did not significantly affect particle elemental composition. Enhanced carbon consumption appears to result in accumulation of dissolved organic carbon under nutrient-recycling summer conditions. This carbon over-consumption effect becomes evident from mass balance calculations, but was too small to be resolved by direct measurements of dissolved organic matter. Faster nutrient uptake by comparatively small algae at high CO2 after nutrient addition resulted in reduced production rates under future ocean CO2 conditions at the end of the experiment. This CO2 mediated shift towards smaller phytoplankton and enhanced cycling of dissolved matter restricted the development of larger phytoplankton, thus pushing the system towards a retention type food chain with overall negative effects on export potential.

Description: Understanding the pre-anthropogenic Pb cycle of central North Pacific deep water has attracted a lot of attention in recent years, partly because of its unique geographical location in that it is a remote gyre system characterized by high dust fluxes and sluggish overturning circulation. However, the factors controlling Pb isotope evolution in this area over the Cenozoic are still controversial and various mechanisms have been proposed in previous studies. Here we report new Pb and Nd isotope time series of four ferromanganese crusts (two from the western Pacific near the Mariana arc and the other two from the central Pacific). Together with previously published records, we discuss for the first time the significance of a persistent and systematic Pb isotopic provinciality recorded by central North Pacific crusts over the Cenozoic. We propose that globally well mixed stratosphere volcanic aerosols could contribute Pb but have not been the major factors controlling the Pb isotope distribution in the central North Pacific over time. Island arc input (and probably enhanced hydrothermal input between about 45 and 20 Ma) likely controlled the Pb isotope provinciality and evolution prior to ~20 Ma, when coeval Pb isotope records in different crusts showed large differences and atmospheric silicate dust flux was extremely low. After the Eocene, in particular after 20 Ma, Asian dust input has become an isotopically resolvable source, while island arc-derived Pb has remained important to balance the dust input and to produce the observed Pb isotope distribution in the central North Pacific during this period.

Description: A realistic primitive-equation model of the Southern Ocean at eddying spatial resolution is used to examine the effect of ocean-surface-velocity dependence of the wind stress on the strength of near-inertial oscillations. Accounting for the ocean-surface-velocity dependence of the wind stress leads to a large reduction of wind-induced near-inertial energy of approximately 40 percent and of wind power input into the near-inertial frequency band of approximately 20 percent. A large part of this reduction can be explained by the leading-order modification to the wind stress if the ocean-surface velocity is included. The strength of the reduction is shown to be modulated by the inverse of the ocean-surface-mixed-layer depth. We conclude that the effect of surface-velocity dependence of the wind stress should be taken into account when estimating the wind-power input into the near-inertial frequency band and when estimating near-inertial energy levels in the ocean due to wind forcing.

Description: Here we report on data from an oceanographic cruise on the German research vessel Meteor covering large parts of the Mediterranean Sea during spring of 2011. The main objectives of this cruise was to conduct measurements of physical, chemical and biological variables on a section across the Mediterranean Sea with the goal of producing a synoptic picture of the distribution of relevant physical and biogeochemical properties, in order to compare those to historic data sets. During the cruise, a comprehensive data set of relevant variables following the guide lines for repeat hydrography outlined by the GO-SHIP group (http://www.go-ship.org/) was collected. The measurements include; salinity and temperature (CTD), an over-determined carbonate system, inorganic nutrients, oxygen, transient tracers (CFC-12, SF6), Helium isotopes and tritium, and carbon isotopes. The cruise sampled all major basins of the Mediterranean Sea following roughly an east-to-west section from the coast of Lebanon to through the Strait of Gibraltar, and to the coast of Portugal. Also a south-to-north section from the Ionian Sea to the Adriatic Sea was carried out. Additionally, sampling in the Aegean, Adriatic and Tyrrhenian Seas were carried out. The sections roughly followed lines and positions that have been sampled previously during other programs, thus providing the opportunity for comparative investigations of the temporal development of various parameters.

Description: The history of climate modeling begins with conceptual models, followed in the 19th century by mathematical models of energy balance and radiative transfer, as well as simple analog models. Since the 1950s, the principal tools of climate science have been computer simulation models of the global general circulation. From the 1990s to the present, a trend toward increasingly comprehensive coupled models of the entire climate system has dominated the field. Climate model evaluation and intercomparison is changing modeling into a more standardized, modular process, presenting the potential for unifying research and operational aspects of climate science

Description: This study presents a new estimate of the oceanic anthropogenic CO2(Cant) sink over the industrial era (1780 to present), from assimilation of potential temperature, salinity, radiocarbon, and CFC-11 observations in a global steady state ocean circulation inverse model (OCIM). This study differs from previous data-based estimates of the oceanic Cant sink in that dynamical constraints on ocean circulation are accounted for, and the ocean circulation is explicitly modeled, allowing the calculation of oceanic Cant storage, air-sea fluxes, and transports in a consistent manner. The resulting uncertainty of the OCIM-estimated Cant uptake, transport, and storage is significantly smaller than the comparable uncertainty from purely data-based or model-based estimates. The OCIM-estimated oceanic Cant storage is 160–166 PgC in 2012, and the oceanic Cant uptake rate averaged over the period 2000–2010 is 2.6 PgC yr−1 or about 30% of current anthropogenic CO2 emissions. This result implies a residual (primarily terrestrial) Cant sink of about 1.6 PgC yr−1 for the same period. The Southern Ocean is the primary conduit for Cant entering the ocean, taking up about 1.1 PgC yr−1 in 2012, which represents about 40% of the contemporary oceanic Cant uptake. It is suggested that the most significant source of remaining uncertainty in the oceanic Cant sink is due to potential variability in the ocean circulation over the industrial era.

Description: Contemporary genetic diversity is the product of both historical and contemporary forces, such as climatic and geological processes affecting range distribution and continuously moulded by evolutionary forces selection, gene flow and genetic drift. Predatory freshwater fishes, such as Northern Pike Esox lucius, commonly exhibit small population sizes, and several local populations are considered endangered. Pike inhabit diverse habitat types, including lakes, rivers and brackish marine waters, thus spanning from small isolated patches to large open marine systems. However, pike population structure from local to regional scales is relatively poorly described, in spite of its significance to developing conservation measures. We analysed microsatellite variation in a total of 1185 North European pike from 46 samples collected across both local and regional scales, as well as over time, to address two overarching questions: Is pike population structure associated with local and/or regional connectivity patterns, and which factors likely have the main influence on the contemporary distribution of genetic diversity? To answer this, we combined estimators of population diversity and structure to assess evidence of whether populations within (i) habitats, (ii) drainage systems and (iii) geographical regions are closer related than among these ranges, and whether patterns are temporally stable. Contrasting previous predictions that genetic drift obscures signals of postglacial colonisation history, we identified clear regional differences in population genetic signatures, suggesting a major effect of drainage divides on colonisation history and connectivity. However, several populations deviated from the general pattern, showing that local processes may be complex and need to be examined case-by-case.

Description: Gas hydrate-related bottom-simulating reflectors mark the phase boundary between hydrate and free gas in the subsurface, and therefore may be used to estimate geothermal gradients and hence heat flow. The depth and temperature of the phase boundary depend on the composition of the hydrateforming gas and of the pore fluid. In the absence of direct sampling, these compositions remain unknown. We develop an alternative approach that is less sensitive to compositional uncertainties and can be applied when the bottom-simulating reflector is densely sampled in a region with significant seabed relief. We apply this approach to a three-dimensional seismic dataset from the eastern Black Sea.

Description: Stratospheric ozone depletion and emission of greenhouse gases lead to a trend of the southern annular mode (SAM) toward its high-index polarity. The positive phase of the SAM is characterized by stronger than usual westerly winds that induce changes in the physical carbon transport. Changes in the natural carbon budget of the upper 100 m of the Southern Ocean in response to a positive SAM phase are explored with a coupled ecosystem-general circulation model and regression analysis. Previously overlooked processes that are important for the upper ocean carbon budget during a positive SAM period are identified, namely, export production and downward transport of carbon north of the polar front (PF) as large as the upwelling in the south. The limiting micronutrient iron is brought into the surface layer by upwelling and stimulates phytoplankton growth and export production but only in summer. This leads to a drawdown of carbon and less summertime outgassing (or more uptake) of natural CO2. In winter, biological mechanisms are inactive, and the surface ocean equilibrates with the atmosphere by releasing CO2. In the annual mean, the upper ocean region south of the PF loses more carbon by additional export production than by the release of CO2 into the atmosphere, highlighting the role of the biological carbon pump in response to a positive SAM event.

Description: We combined data sets of measured sedimentary calcium carbonate (CaCO3) and satellite-derived pelagic primary production to parameterize the relation between CaCO3 content on the Antarctic shelves and primary production in the overlying water column. CaCO3 content predicted in this way was in good agreement with the measured data. The parameterization was then used to chart CaCO3 content on the Antarctic shelves all around the Antarctic, using the satellite-derived primary production. The total inventory of CaCO3 in the bioturbated layer of Antarctic shelf sediments was estimated to be 0.5 Pg C. This quantity is comparable to the total CO2 uptake by the Southern Ocean in only one to a few years (dependent on the uptake estimate and area considered), indicating that the dissolution of these carbonates will neither delay ocean acidification in this area nor augment the Southern Ocean CO2 uptake capacity.

Description: Before making a critical evaluation of the crude oil and natural gas prospects for the years to the end of the century, it is necessary to review the geology and structure of the three German hydrocarbon-producing provinces. Furthermore, past exploration, production and reserves should be discussed. The three hydrocarbon-producing provinces are: the NW German Basin, the Upper Rhine Graben and the Molasse Basin, which together make up about 41% of West German territory (Fig. 1). The NW German Basin contains a sedimentary sequence over 8,000 m thick ranging in age from Permian to Quaternary. Gas and oil, the two natural hydrocarbons, are generally confined to separate lower and higher stratigraphic levels respectively (Fig. 2). The NW German Basin is the most important prospective area in West Germany. It extends into the North Sea. The tectonic rift feature of the Upper Rhine Graben originated in the Eocene. The Tertiary fill is over 4,000 m thick. Oil is found mainly in Mesozoic, Eocene and Oligocene rocks; the Miocene and Pliocene reservoir rocks contain natural gas almost exclusively (Fig. 3). The Molasse Basin is part of the foredeep north of the Alpine and Carpathian mountain ranges. The basin is filled with Upper Eocene to Pliocene and Quaternary sediments which, near the Alpine nappes, reach a thickness of over 5,000m (Fig. 4). During this century there were peaks in annual oil-production in 1910, 1940 and 1968 (see Fig. 5). The 1910 peak was the result of drilling activity in the Wietze oilfield. During the period 1934–1945, government financial aid was made available for drilling exploration wells. The success of this collaboration is demonstrated by the oil output in 1940 of 1 × 106 t. After World War II, many different types of oil-bearing structure were found, particularly by reflection seismic techniques in conjunction with detailed stratigraphical and palaeogeographical investigations. The success achieved can be seen by the peak of 8 × 106 t oil production for 1968 (Fig. 5) and in the growth of oil reserves (Fig. 7). Intensive exploration also enabled many new gasfields to be developed, especially in the deeper horizons of the NW German Basin. In 1971, estimated gas reserves reached 360 × 109 m3 (Fig. 11), and annual gas production in 1979 was 20.7 × 109 m3 (731 Bcf) (Fig. 9). There is, no doubt, still scope for the discovery and exploitation of oil and gas in Germany, especially in the NW German Basin where the best prospects for the future lie. This is borne out by two recent offshore oil discoveries and also by the successful application of enhanced recovery methods in the oilfields. The chances of finding more gas at the lower stratigraphic levels are promising now that gas has been discovered in the deeper parts of the Permian basin. The results of massive-hydraulic-fracturing tests in low-permeability pay-horizons are also encouraging. The deeper parts of oil- and gas-producing basins contain interesting prospects and have yet to be tested by ultra-deep wells. Provided that the economic climate remains favourable, there should be no difficulty in finding and supplying German oil and gas in the future. Geologically and technically possible reserves should be converted into proven and/or probable reserves. German crude oil will be available for several years beyond the year 2000, and German natural gas for a far longer time. A production rate of 19 to 20 × 109 m3 of gas per annum is feasible over the next twenty years, and oil production will probably not sink below 3 × 106 t/a in this period.

Description: Correlations between particulate organic carbon (POC) and mineral fluxes in the deep ocean have inspired the inclusion of “ballast effect” parameterizations in carbon cycle models. A recent study demonstrated regional variability in the effect of ballast minerals on the flux of POC in the deep ocean. We have undertaken a similar analysis of shallow export data from the Arctic, Atlantic, and Southern Oceans. Mineral ballasting is of greatest importance in the high-latitude North Atlantic, where 60% of the POC flux is associated with ballast minerals. This fraction drops to around 40% in the Southern Ocean. The remainder of the export flux is not associated with minerals, and this unballasted fraction thus often dominates the export flux. The proportion of mineral-associated POC flux often scales with regional variation in export efficiency (the proportion of primary production that is exported). However, local discrepancies suggest that regional differences in ecology also impact the magnitude of surface export. We propose that POC export will not respond equally across all high-latitude regions to possible future changes in ballast availability.

Description: Because the vast majority of species are well diverged, relatively little is known about the genomic architecture of speciation during the early stages of divergence. Species within recent evolutionary radiations are often minimally diverged from a genomic perspective, and therefore provide rare opportunities to address this question. Here, we leverage the hamlet radiation (Hypoplectrus spp., brightly coloured reef fishes from the tropical western Atlantic) to characterize genomic divergence during the early stages of speciation. Transect surveys and spawning observations in Belize, Honduras and Panama confirm that sympatric barred (H. puella), black (H. nigricans) and butter (H. unicolor) hamlets are phenotypically distinct and reproductively isolated, although hybrid spawnings and individuals with intermediate phenotypes are seen on rare occasions. A survey of approximately 100 000 restriction site-associated SNPs in 126 samples from the three species across the three replicate populations reveals extremely slight genomewide divergence among species (FST = 0.0038), indicating that ecomorphological differences and functional reproductive isolation are maintained in sympatry in a backdrop of extraordinary genomic similarity. Nonetheless, a very small proportion of SNPs (0.05% on average) are identified as FST outliers among sympatric species. Remarkably, a single SNP is identified as an outlier in repeated populations for the same species pair. A minicontig assembled de novo around this SNP falls into the genomic region containing the HoxCa10 and HoxCa11 genes in 10 teleost species, suggesting an important role for Hox gene evolution in this radiation. This finding, if confirmed, would provide a better understanding of the links between micro- and macroevolutionary processes.

Description: Linking lower and higher trophic levels requires special focus on the essential role played by mid-trophic levels, i.e., the zooplankton. One of the most relevant pieces of information regarding zooplankton in terms of flux of energy lies in its size structure. In this study, an extensive data set of size measurements is presented, covering parts of the western European continental shelf and slope, from the Galician coast to the Ushant front, during the springs from 2005 to 2012. Zooplankton size spectra were estimated using measurements carried out in situ with the Laser Optical Plankton Counter (LOPC) and with an image analysis of WP2 net samples (200 μm mesh size) performed following the ZooScan methodology. The LOPC counts and sizes particles within 100–2000 μm of spherical equivalent diameter (ESD), whereas the WP2/ZooScan allows for counting, sizing and identification of zooplankton from ~ 400 μm ESD. The difference between the LOPC (all particles) and the WP2/ZooScan (zooplankton only) was assumed to provide the size distribution of non-living particles, whose descriptors were related to a set of explanatory variables (including physical, biological and geographic descriptors). A statistical correction based on these explanatory variables was further applied to the LOPC size distribution in order to remove the non-living particles part, and therefore estimate the size distribution of zooplankton. This extensive data set provides relevant information about the zooplankton size distribution variability, productivity and trophic transfer efficiency in the pelagic ecosystem of the Bay of Biscay at a regional and interannual scale.

Description: The oceanic biological carbon pump is an important factor in the global carbon cycle. Organic carbon is exported from the surface ocean mainly in the form of settling particles derived from plankton production in the upper layers of the ocean. The large variability in current estimates of the global strength of the biological carbon pump emphasises that our knowledge of a major planetary carbon flux remains poorly constrained. We present a database of 723 estimates of organic carbon export from the surface ocean derived from the 234Th technique. The dataset is archived on the data repository PANGEA® (www.pangea.de) under doi:10.1594/PANGAEA.809717. Data were collected from tables in papers published between 1985 and early 2013. We also present sampling dates, publication dates and sampling areas. Most of the open ocean provinces are represented by multiple measurements. However, the western Pacific, the Atlantic Arctic, South Pacific and the southern Indian Ocean are not well represented. There is a variety of integration depths ranging from surface to 300 m. Globally the fluxes ranged from 0 to 1500 mg C m−2 d−1.

Description: Variability and trends in seasonal and interannual ice area export out of the Laptev Sea between 1992 and 2011 are investigated using satellite-based sea ice drift and concentration data. We found an average total winter (October to May) ice area transport across the northern and eastern Laptev Sea boundaries (NB and EB) of 3.48 × 10hoch5 km2. The average transport across the NB (2.87 × 10hoch5 km2)is thereby higher than across the EB (0.61 × 10hoch5 km2), with a less pronounced seasonal cycle. The total Laptev Sea ice area flux significantly increased over the last decades (0.85 × 10hoch5 km2 decade−1, p 〉 0.95), dominated by increasing export through the EB (0.55 × 10hoch5 km2 decade−1, p 〉 0.90), while the increase in export across the NB is smaller (0.3 × 10hoch5 km2 decade−1) and statistically not significant. The strong coupling between across-boundary SLP gradient and ice drift velocity indicates that monthly variations in ice area flux are primarily controlled by changes in geostrophic wind velocities, although the Laptev Sea ice circulation shows no clear relationship with large-scale atmospheric indices. Also there is no evidence of increasing wind velocities that could explain the overall positive trends in ice export. The increased transport rates are rather the consequence of a changing ice cover such as thinning and/or a decrease in concentration. The use of a back-propagation method revealed that most of the ice that is incorporated into the Transpolar Drift is formed during freeze-up and originates from the central and western part of the Laptev Sea, while the exchange with the East Siberian Sea is dominated by ice coming from the central and southeastern Laptev Sea. Furthermore, our results imply that years of high ice export in late winter (February to May) have a thinning effect on the ice cover, which in turn preconditions the occurence of negative sea ice extent anomalies in summer.

Description: To examine the potentially competing influences of microzooplankton and calcite mineral ballast on organic matter remineralization, we incubated diatoms in darkness in rolling tanks with and without added calcite minerals (coccoliths) and microzooplankton (rotifers). Concentrations of particulate organic matter (POM in suspension or in aggregates), of dissolved organic matter (DOM), and of dissolved inorganic nutrients were monitored over 8 days. The presence of rotifers enhanced the remineralization of ammonium and phosphate, but not dissolved silicon, from the biogenic particulate matter, up to 40% of which became incorporated into aggregates early in the experiment. Added calcite resulted in rates of excretion of ammonium and phosphate by rotifers that were depressed by 67% and 36%, respectively, demonstrating the potential for minerals to inhibit the destruction of POM by zooplankton in the water column. Lastly, the presence of the rotifers and added calcite minerals resulted in a more rapid initial rate of aggregation, although not a greater overall amount of aggregation during the experiment.

Description: Meridional and vertical distributions of several biogeochemical parameters were studied along a section in the southeastern Atlantic and the Southern Ocean south of South Africa during the austral summer 2008 of the International Polar Year to characterize the biogeochemical provinces and to assess the seasonal net diatom production. Based on analyses of macro-nutrients, ammonium (NH4), chlorophyll a, (Chl a), phaeopigments, biogenic silica (BSi), particulate inorganic carbon (PIC), and particulate organic carbon and nitrogen (POC and PON, respectively), four biogeochemical domains were distinguished along the section: the subtropical Atlantic, the confluence zone of the subtropical and subantarctic domains, the Polar Frontal Zone (PFZ) in the Antarctic Circumpolar Current (ACC), and the north-eastern branch of the Weddell Gyre. The subtropical region displayed extremely low nutrient concentrations featuring oligotrophic conditions, and sub-surface maxima of Chl a and phaeopigments never exceeded 0.5 µg L−1 and 0.25 µg L−1, respectively. The anticyclonic and cyclonic eddies crossed in the Cape Basin were characterized by a deepening and a rise, respectively, of the nutrients isoclines. The confluence zone of the subtropical domain and the northern side of the ACC within the subantarctic domain displayed remnant nitrate and phosphate levels, whereas silicate concentrations kept to extremely low levels. In this area, Chl a level of 0.4–0.5 µg L−1 distributed homogenously within the mixed layer, and POC and PON accumulated to values up to 10 µM and 1.5 µM, respectively, indicative of biomass accumulation along the confluence zone during the late productive period. In the ACC domain, the Polar Frontal Zone was marked by a post-bloom of diatoms that extended beyond the Polar Front (PF) during this late summer condition, as primarily evidenced by the massive depletion of silicic acid in the surface waters. The accumulation of NH4 to values up to 1.25 µM at 100 m depth centred on the PF and the accumulation of BSi up to 0.5 µM in the surface waters of the central part of the PFZ also featured a late stage of the seasonal diatom bloom. The silica daily net production rate based on the seasonal depletion of silicic acid was estimated to be 11.9 ± 6.5 mmol m−2 d−1 in the domain of the vast diatom post-bloom, agreeing well with the previously recorded values in this province. The Weddell Gyre occasionally displayed relative surface depletion of silicic acid, suggesting a late stage of a relatively minor diatom bloom possibly driven by iceberg drifting releases of iron. In this domain the estimated range of silica daily net production rate (e.g. 21.1 ± 8.8 mmol m−2 d−1) is consistent with previous studies, but was not significantly higher than that in the Polar Front region.

Description: As part of the Bonus-GoodHope (BGH) campaign, 15N-labelled nitrate, ammonium and urea uptake measurements were made along the BGH transect from Cape Town to ~60° S in late austral summer, 2008. Our results are categorised according to distinct hydrographic regions defined by oceanic fronts and open ocean zones. High regenerated nitrate uptake rate in the oligotrophic Subtropical Zone (STZ) resulted in low f-ratios (f = 0.2) with nitrogen uptake being dominated by ρurea, which contributed up to 70 % of total nitrogen uptake. Size fractionated chlorophyll data showed that the greatest contribution (〉50 %) of picophytoplankton (〈2 μm) were found in the STZ, consistent with a community based on regenerated production. The Subantarctic Zone (SAZ) showed the greatest total integrated nitrogen uptake (10.3 mmol m−2 d−1), mainly due to enhanced nutrient supply within an anticyclonic eddy observed in this region. A decrease in the contribution of smaller size classes to the phytoplankton community was observed with increasing latitude, concurrent with a decrease in the contribution of regenerated production. Higher f-ratios observed in the SAZ (f = 0.49), Polar Frontal Zone (f= 0.41) and Antarctic Zone (f = 0.45) relative to the STZ (f = 0.24), indicate a higher contribution of NO3−-uptake relative to total nitrogen and potentially higher export production. High ambient regenerated nutrient concentrations are indicative of active regeneration processes throughout the transect and ascribed to late summer season sampling. Higher depth integrated uptake rates also correspond with higher surface iron concentrations. No clear correlation was observed between carbon export estimates derived from new production and 234Th flux. In addition, export derived from 15N estimates were 2–20 times greater than those based on 234Th flux. Variability in the magnitude of export is likely due to intrinsically different methods, compounded by differences in integration time scales for the two proxies of carbon export.

Description: In the vast Low Nutrient Low-Chlorophyll (LNLC) Ocean, the vertical nutrient supply from the subsurface to the sunlit surface waters is low, and atmospheric contribution of nutrients may be one order of magnitude greater over short timescales. The short turnover time of atmospheric Fe and N supply (〈1 month for nitrate) further supports deposition being an important source of nutrients in LNLC regions. Yet, the extent to which atmospheric inputs are impacting biological activity and modifying the carbon balance in oligotrophic environments has not been constrained. Here, we quantify and compare the biogeochemical impacts of atmospheric deposition in LNLC regions using both a compilation of experimental data and model outputs. A metadata-analysis of recently conducted field and laboratory bioassay experiments reveals complex responses, and the overall impact is not a simple “fertilization effect of increasing phytoplankton biomass” as observed in HNLC regions. Although phytoplankton growth may be enhanced, increases in bacterial activity and respiration result in weakening of biological carbon sequestration. The application of models using climatological or time-averaged non-synoptic deposition rates produced responses that were generally much lower than observed in the bioassay experiments. We demonstrate that experimental data and model outputs show better agreement on short timescale (days to weeks) when strong synoptic pulse of aerosols deposition, similar in magnitude to those observed in the field and introduced in bioassay experiments, is superimposed over the mean atmospheric deposition fields. These results suggest that atmospheric impacts in LNLC regions have been underestimated by models, at least at daily to weekly timescales, as they typically overlook large synoptic variations in atmospheric deposition and associated nutrient and particle inputs. Inclusion of the large synoptic variability of atmospheric input, and improved representation and parameterization of key processes that respond to atmospheric deposition, is required to better constrain impacts in ocean biogeochemical models. This is critical for understanding and prediction of current and future functioning of LNLC regions and their contribution to the global carbon cycle.

Description: Knowledge of calcium phosphate (Ca-P) solubility is crucial for understanding temporal and spatial variations of phosphorus (P) concentrations in water bodies and sedimentary reservoirs. In situ relationships between liquid-and solid-phase levels cannot be fully explained by dissolved analytes alone and need to be verified by determining particular sediment P species. Lack of quantification methods for these species limits the knowledge of the P cycle. To address this issue, we (i) optimized a specifically developed conversion-extraction (CONVEX) method for P species quantification using standard additions, and (ii) simultaneously determined solubilities of Ca-P standards by measuring their pH-dependent contents in the sediment matrix. Ca-P minerals including various carbonate fluorapatite (CFAP) specimens from different localities, fluorapatite (FAP), fish bone apatite, synthetic hydroxylapatite (HAP) and octacalcium phosphate (OCP) were characterized by XRD, Raman, FTIR and elemental analysis. Sediment samples were incubated with and without these reference minerals and then sequentially extracted to quantify Ca-P species by their differential dissolution at pH values between 3 and 8. The quantification of solid-phase phosphates at varying pH revealed solubilities in the following order: OCP〉 HAP〉 CFAP (4.5% CO3)〉 CFAP (3.4% CO3)〉 CFAP (2.2% CO3)〉 FAP. Thus, CFAP was less soluble in sediment than HAP, and CFAP solubility increased with carbonate content. Unspiked sediment analyses together with standard addition analyses indicated consistent differential dissolution of natural sediment species vs. added reference species and therefore verified the applicability of the CONVEX method in separately determining the most prevalent Ca-P minerals. We found surprisingly high OCP contents in the coastal sediments analyzed, which supports the hypothesis of apatite formation by an OCP precursor mechanism.

Description: Summary 1. Parasitized females in mammals, fish and birds can enhance the immune defence of their offspring by transferring specific antibodies for the embryo. Likewise, social insect mothers transfer immunity despite the fact that invertebrates lack antibodies. 2.  Female trans-generational immune priming is consistent with parental investment theory, because mothers invest more into rearing their offspring than fathers. However, when immune priming is not directly linked to parental care, as is often the case in insects that abandon their eggs after oviposition, both sexes might benefit from protecting their offspring. 3. Using the red flour beetle, Tribolium castaneum, we show that after parental exposure to heat-killed bacteria, trans-generational immune priming occurs through fathers as well as mothers. 4. This novel finding challenges the traditional view that males provide only genes to their offspring in species without paternal care, and raises the possibility of a division of tasks with respect to immune protection between parents.

Description: Here we make an initial step toward the development of an ocean assimilation system that can constrain the modelled Atlantic Meridional Overturning Circulation (AMOC) to support climate predictions. A detailed comparison is presented of 1° and 1/4° resolution global model simulations with and without sequential data assimilation, to the observations and transport estimates from the RAPID mooring array across 26.5° N in the Atlantic. Comparisons of modelled water properties with the observations from the merged RAPID boundary arrays demonstrate the ability of in situ data assimilation to accurately constrain the east-west density gradient between these mooring arrays. However, the presence of an unconstrained "western boundary wedge" between Abaco Island and the RAPID mooring site WB2 (16 km offshore) leads to the intensification of an erroneous southwards flow in this region when in situ data are assimilated. The result is an overly intense southward upper mid-ocean transport (0–1100 m) as compared to the estimates derived from the RAPID array. Correction of upper layer zonal density gradients is found to compensate mostly for a weak subtropical gyre circulation in the free model run (i.e. with no assimilation). Despite the important changes to the density structure and transports in the upper layer imposed by the assimilation, very little change is found in the amplitude and sub-seasonal variability of the AMOC. This shows that assimilation of upper layer density information projects mainly on the gyre circulation with little effect on the AMOC at 26° N due to the absence of corrections to density gradients below 2000 m (the maximum depth of Argo). The sensitivity to initial conditions was explored through two additional experiments using a climatological initial condition. These experiments showed that the weak bias in gyre intensity in the control simulation (without data assimilation) develops over a period of about 6 months, but does so independently from the overturning, with no change to the AMOC. However, differences in the properties and volume transport of North Atlantic Deep Water (NADW) persisted throughout the 3 year simulations resulting in a difference of 3 Sv in AMOC intensity. The persistence of these dense water anomalies and their influence on the AMOC is promising for the development of decadal forecasting capabilities. The results suggest that the deeper waters must be accurately reproduced in order to constrain the AMOC.

Description: Since April 2004 the RAPID array has made continuous measurements of the Atlantic Meridional Overturning Circulation (AMOC) at 26° N. Two key components of this system are Ekman transport zonally integrated across 26° N and western boundary current transport in the Florida Straits. Whilst measurements of the AMOC as a whole are somewhat in their infancy, this study investigates what useful information can be extracted on the variability of the Ekman and Florida Straits transports using the decadal timeseries already available. Analysis is also presented for Sverdrup transports zonally integrated across 26° N. The seasonal cycles of Florida Straits, Ekman and Sverdrup transports are quantified at 26° N using harmonic analysis of annual and semi-annual constituents. Whilst Sverdrup transport shows clear semi-annual periodicity, calculations of seasonal Florida Straits and Ekman transports show substantial interannual variability due to contamination by variability at non-seasonal frequencies; the mean seasonal cycle for these transports only emerges from decadal length observations. The Florida Straits and Ekman mean seasonal cycles project on the AMOC with a combined peak-to-peak seasonal range of 3.5 Sv. The combined seasonal range for heat transport is 0.40 PW. The Florida Straits seasonal cycle possesses a smooth annual periodicity in contrast with previous studies suggesting a more asymmetric structure. No clear evidence is found to support significant changes in the Florida Straits seasonal cycle at sub-decadal periods. Whilst evidence of wind driven Florida Straits transport variability is seen at sub-seasonal and annual periods, a model run from the 1/4° eddy-permitting ocean model NEMO is used to identify an important contribution from internal oceanic variability at sub-annual and interannual periods. The Ekman transport seasonal cycle possesses less symmetric structure, due in part to different seasonal transport regimes east and west of 50 to 60° W. Around 60% of non-seasonal Ekman transport variability occurs in phase section-wide at 26° N and is related to the NAO, whilst Sverdrup transport variability is more difficult to decompose.

Description: Marine macroalgae are constantly exposed to epibacterial colonizers. The epiphytic bacterial patterns and their temporal and spatial variability on host algae are poorly understood. To investigate the interaction between marine macroalgae and epiphytic bacteria, this study tested if the composition of epibacterial communities on different macroalgae was specific and persisted under varying biotic and abiotic environmental conditions over a 2-year observation time frame. Epibacterial communities on the co-occurring macroalgae Fucus vesiculosus, Gracilaria vermiculophylla and Ulva intestinalis were repeatedly sampled in summer and winter of 2007 and 2008. The epibacterial community composition was analysed by denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene libraries. Epibacterial community profiles did not only differ significantly at each sampling interval among algal species, but also showed consistent seasonal differences on each algal species at a bacterial phylum level. These compositional patterns re-occurred at the same season of two consecutive years. Within replicates of the same algal species, the composition of bacterial phyla was subject to shifts at the bacterial species level, both within the same season but at different years and between different seasons. However, 7–16% of sequences were identified as species specific to the host alga. These findings demonstrate that marine macroalgae harbour species-specific and temporally adapted epiphytic bacterial biofilms on their surfaces. Since several algal host-specific bacteria were highly similar to other bacteria known to either avoid subsequent colonization by eukaryotic larvae or to exhibit potent antibacterial activities, algal host-specific bacterial associations are expected to play an important role for marine macroalgae.

Description: A wind-driven meso-scale pattern of temperature, salinity and oxygen was found along a transect in the northern Bornholm Basin (southern Baltic Sea). Strong winds caused currents along this transect, which shifted cold intermediate water (minimum: 3.6C) towards the south. The transect was surveyed with a towed CTD-system and hydroacoustics in parallel to investigate the distribution of sprat, Sprattus sprattus balticus (Schn.) in relation to the observed meso-scale pattern. In those parts of the transect where the cold intermediate water was observed, sprat were restricted to water layers below the halocline. In other parts of the transect, sprat moved into higher water layers and occupied a wider depth range. The important factor was temperature, which set an upper limit to the vertical sprat distribution. The development of hydrography, as measured in the field, was evaluated with a hydrodynamic model.

Description: During the last decade, global surface temperatures did not increase as rapidly as in the preceding decades. Although relatively small compared to the observed centennial scale global warming, it has renewed interest in understanding and even predicting climate on time scales of decades, and sparked a community initiative on near‐term prediction that will feature in the fifth intergovernmental panel on climate change assessment report. Decadal prediction, however, is in its infancy, with only a few publications existing. This article has three aims. The first is to make the case for decadal prediction. Decadal fluctuations in global climate similar to that of recent decades were observed during the past century. Associated with large regional changes in precipitation and climate extremes, they are of socioeconomic importance. Climate models, which capture some aspects of observed decadal variability, indicate that such variations might be partly predictable. The second aim is to describe the major challenges to skilful decadal climate prediction. One is poor understanding of mechanisms of decadal climate variability, with climate models showing little agreement. Sparse observations in the past, particularly in the ocean, are also a limiting factor to developing and testing of initialization and prediction systems. The third aim is to stress that despite promising initial results, decadal prediction is in a highly experimental stage, and care is needed in interpreting results and utilizing data from such experiments. In the long‐term, decadal prediction has the potential to improve models, reduce uncertainties in climate change projections, and be of socioeconomic benefit. Copyright © 2010 John Wiley & Sons, Ltd.

Description: Seawater concentrations of the four brominated trace gases dibromomethane (CH2Br2), bromodichloromethane (CHBrCl2), dibromochloromethane (CHBr2Cl) and bromoform (CHBr3) were measured at different depths of the water column in the Iberian upwelling off Portugal during summer 2007. Bromocarbon concentrations showed elevated values in recently upwelled and aged upwelled waters (mean values of 30 pmol L−1 for CHBr3), while values in the open ocean were significantly lower (7.4 pmol L−1 for CHBr3). Correlations with biological variables and marker pigments indicated that phytoplankton could be identified as a weak bromocarbon source in the open ocean. In upwelled water masses along the coast, halocarbons were not correlated to Chl-a, indicating an external source, overlapping the possible internal production by phytoplankton. We showed that the tidal frequency had a significant influence on halocarbon concentrations in the upwelling and we linked those findings to a strong intertidal coastal source, as well as to a transport of those halocarbon enriched coastal waters by westward surface upwelling currents. Coastal sources and transport can be accounted for maximum values of up to 185.1 pmol L−1 CHBr3 in the upwelling. Comparison with other productive marine areas revealed that the Iberian upwelling had stronger halocarbon sources than the phytoplankton dominated sources in the Mauritanian upwelling. However, the concentrations off the Iberian Peninsula were still much lower than those of coastal macroalgal influenced waters or those of polar regions dominated by cold water adapted diatoms

Description: A steady state box model was developed to estimate the methane input into the Black Sea water column at various water depths. Our model results reveal a total input of methane of 4.7 Tg yr−1. The model predicts that the input of methane is largest at water depths between 600 and 700 m (7% of the total input), suggesting that the dissociation of methane gas hydrates at water depths equivalent to their upper stability limit may represent an important source of methane into the water column. In addition we discuss the effects of massive short-term methane inputs (e.g. through eruptions of deep-water mud volcanoes or submarine landslides at intermediate water depths) on the water column methane distribution and the resulting methane emission to the atmosphere. Our non-steady state simulations predict that these inputs will be effectively buffered by intense microbial methane consumption and that the upward flux of methane is strongly hampered by the pronounced density stratification of the Black Sea water column. For instance, an assumed input of methane of 179 Tg CH4 d−1 (equivalent to the amount of methane released by 1000 mud volcano eruptions) at a water depth of 700 m will only marginally influence the sea/air methane flux increasing it by only 3%.

Description: Environmental perturbation, climate change and international commerce are important drivers for biological invasions. Climate anomalies can further increase levels of habitat disturbance and act synergistically to elevate invasion risk. Herein, we use a historical data set from the upper San Francisco Estuary to provide the first empirical evidence for facilitation of invasions by climate extremes. Invasive zooplankton species did not become established in this estuary until the 1970s when increasing propagule pressure from Asia coincided with extended drought periods. Hydrological management exacerbated the effects of post-1960 droughts and reduced freshwater inflow even further, increasing drought severity and allowing unusually extreme salinity intrusions. Native zooplankton experienced unprecedented conditions of high salinity and intensified benthic grazing, and life history attributes of invasive zooplankton were advantageous enough during droughts to outcompete native species and colonise the system. Extreme climatic events can therefore act synergistically with environmental perturbation to facilitate the establishment of invasive species.

Description: Recent seismic evidence suggested that most oceanic plate hydration is associated with trench-outer rise faulting prior to subduction. Hydration at trenches may have a significant impact on the subduction zone water cycle. Previous seismic experiments conducted to the northwest of Nicoya Peninsula, Northern Costa Rica, have shown that the subducting Cocos lithosphere is pervasively altered, which was interpreted to be due to both hydration (serpentinization) and fracturing of the crustal and upper-mantle rocks. New seismic wide-angle reflection and refraction data were collected along two profiles, running parallel to the Middle American trench axis offshore of central Nicaragua, revealing lateral changes of the seismic properties of the subducting lithosphere. Seismic structure along both profiles is characterized by low velocities both in the crust and upper mantle. Velocities in the uppermost mantle are found to be in the range 7.3–7.5 km s−1; thus are 8–10 per cent lower than velocities typical for unaltered peridotites and hence confirm the assumption that serpentinization is a common process at the trench-outer rise area offshore of Nicaragua. In addition, a prominent velocity anomaly occurred within the crust beneath two seamounts. Here, velocity reduction may indicate increased porosity and perhaps permeability, supporting the idea that seamounts serve as sites for water percolation and circulation.

Description: The rugged submarine topography of the Azores supports a diverse heterozoan association resulting in intense biotically-controlled carbonate-production and accumulation. In order to characterise this cold-water (C) factory a 2-year experiment was carried out in the southern Faial Channel to study the biodiversity of hardground communities and for budgeting carbonate production and degradation along a bathymetrical transect from the intertidal to bathyal 500 m depth. Seasonal temperatures peak in September (above a thermocline) and bottom in March (stratification diminishes) with a decrease in amplitude and absolute values with depth, and tidal-driven short-term fluctuations. Measured seawater stable isotope ratios and levels of dissolved nutrients decrease with depth, as do the calcium carbonate saturation states. The photosynthetic active radiation shows a base of the euphotic zone in ~70 m and a dysphotic limit in ~150 m depth. Bioerosion, being primarily a function of light availability for phototrophic endoliths and grazers feeding upon them, is ~10 times stronger on the illuminated upside versus the shaded underside of substrates in the photic zone, with maximum rates in the intertidal (−631 g/m2/yr). Rates rapidly decline towards deeper waters where bioerosion and carbonate accretion are slow and epibenthic/endolithic communities take years to mature. Accretion rates are highest in the lower euphotic zone (955 g/m2/yr), where the substrate is less prone to hydrodynamic force. Highest rates are found – inversely to bioerosion – on down-facing substrates, suggesting that bioerosion may be a key factor governing the preferential settlement and growth of calcareous epilithobionts on down-facing substrates. In context of a latitudinal gradient, the Azores carbonate cycling rates plot between known values from the cold-temperate Swedish Kosterfjord and the tropical Bahamas, with a total range of two orders in magnitude. Carbonate budget calculations for the bathymetrical transect yield a mean 266.9 kg of epilithic carbonate production, −54.6 kg of bioerosion, and 212.3 kg of annual net carbonate production per metre of coastline in the Azores C factory.

Description: Wide-angle seismic data have been used to determine the velocity and density structure of the crust and uppermost mantle beneath the Cape Verdes mid-plate swell. Seismic modelling reveals a ‘standard’ oceanic crust, ∼8 km in thickness, with no direct evidence for low-density bodies at the base of the crust. Gravity anomaly modelling within the constraints and resolution provided by the seismic model, does not preclude, however, a layer of crustal underplate up to 3 km thick beneath the swell crest. The modelling shows that while the seismically constrained crustal structure accounts for the short-wavelength free-air gravity anomaly, it fails to fully explain the long-wavelength anomaly. The main discrepancy is over the swell crest where the gravity anomaly, after correcting for crustal structure, is higher by about 30 mGal than it is over its flanks. The higher gravity can be explained if the top 100 km of the mantle beneath the swell crest is less dense than its surroundings by 30 kg m−3. The lack of evidence for low densities and velocities in the uppermost mantle, and high densities and velocities in the lower crust, suggests that neither a depleted swell root or crustal underplate are the origin of the observed shallower-than-predicted bathymetry and that, instead, the swell is most likely supported by dynamic uplift associated with an anomalously low density asthenospheric mantle.

Description: Indoor mesocosms were used to study the combined effect of warming and of different densities of overwintering mesozooplankton (mainly copepods) on the spring development of phytoplankton in shallow, coastal waters. Similar to previous studies, warming accelerated the spring phytoplankton peak by ca. 1 day 1C1 whereas zooplankton did not significantly influence timing. Phytoplankton biomass during the experimental period decreased with warming and with higher densities of overwintering zooplankton. Similarly, average cell size and average effective particle size (here: colony size) decreased both with zooplankton density and warming. A decrease in phytoplankton particle size is generally considered at typical footprint of copepod grazing. We conclude that warming induced changes in the magnitude and structure of the phytoplankton spring bloom cannot be understood without considering grazing by overwintering zooplankton.

Description: Aim: We investigate the relationship between local and regional richness in marine fouling assemblages using an expanded and globally replicated approach by incorporating two dimensions of diversity (taxonomic and functional) and different successional stages. Location: Global. Methods: In eight different biogeographic regions (Australia, Brazil, Chile, England, Italy, Japan, Portugal and Sweden) 68 polyvinylchloride (PVC) panels (15 × 15 × 0.3 cm) were deployed for colonization. Communities colonizing panels were analysed by measuring percentage cover at each of four different successional ages: 2, 4, 6 and 8 months. Local richness was assessed as the average number of species and functional groups (FGs) per panel and regional richness was evaluated as the estimated (Jack2) asymptote of the sample-accumulation curves for species and FG on experimental panels. Results: We found that the shape of the relationship between local and regional richness depended on successional stage and the type of richness considered, i.e. taxonomic or functional richness. Hardly any relationship was detectable between local taxonomic richness and regional taxonomic richness at any successional stage. In contrast, the relation between local functional and regional functional richness shows a unimodal pattern of change during succession, passing through the stages ‘independent’, ‘unsaturated rising’, ‘saturated rising’ and once again ‘independent’. Main conclusions: The relationship between local and regional richness, whether taxonomic or functional, frequently displays independence of the two scales, particularly in early and late phases of the successional process.

Description: A simple prognostic tool for gas hydrate (GH) quantification in marine sediments is presented based on a diagenetic transport-reaction model approach. One of the most crucial factors for the application of diagenetic models is the accurate formulation of microbial degradation rates of particulate organic carbon (POC) and the coupled formation of biogenic methane. Wallmann et al. (2006) suggested a kinetic formulation considering the ageing effects of POC and accumulation of reaction products (CH4, CO2) in the pore water. This model is applied to data sets of several ODP sites in order to test its general validity. Based on a thorough parameter analysis considering a wide range of environmental conditions, the POC accumulation rate (POCar in g/m2/yr) and the thickness of the gas hydrate stability zone (GHSZ in m) were identified as the most important and independent controls for biogenic GH formation. Hence, depth-integrated GH inventories in marine sediments (GHI in g of CH4 per cm2 seafloor area) can be estimated as: GHI=a ·POCar·GHSZb ·exp(−GHSZc/POCar/d)+e with a = 0.00214, b = 1.234, c = −3.339, d = 0.3148, e = −10.265. The transfer function gives a realistic first order approximation of the minimum GH inventory in low gas flux (LGF) systems. The overall advantage of the presented function is its simplicity compared to the application of complex numerical models, because only two easily accessible parameters need to be determined.

Description: Novel high-pressure biotechnical systems that were developed and applied for the study of anaerobic oxidation of methane (AOM) are described. The systems, referred to as high-pressure continuous incubation system (HP-CI system) and high-pressure manifold-incubation system (HP-MI system), allow for batch, fed-batch, and continuous gas-phase free incubation at high concentrations of dissolved methane and were designed to meet specific demands for studying environmental regulation and kinetics as well as for enriching microbial biomass in long-term incubation. Anoxic medium is saturated with methane in the first technical stage, and the saturated medium is supplied for biomass incubation in the second stage. Methane can be provided in continuous operation up to 20 MPa and the incubation systems can be operated during constant supply of gas-enriched medium at a hydrostatic pressure up to 45 MPa. To validate the suitability of the high-pressure systems, we present data from continuous and fed-batch incubation of highly active samples prepared from microbial mats from the Black Sea collected at a water depth of 213 m. In continuous operation in the HP-CI system initial methane-dependent sulfide production was enhanced 10- to 15-fold after increasing the methane partial pressure from near ambient pressure of 0.2 to 10.0 MPa at a hydrostatic pressure of 16.0 MPa in the incubation stage. With a hydraulic retention time of 14 h a stable effluent sulfide concentration was reached within less than 3 days and a continuing increase of the volumetric AOM rate from 1.2 to 1.7 mmol L−1 day−1 was observed over 14 days. In fed-batch incubation the AOM rate increased from 1.5 to 2.7 and 3.6 mmol L−1 day−1 when the concentration of aqueous methane was stepwise increased from 5 to 15 mmol L−1 and 45 mmol L−1. A methane partial pressure of 6 MPa and a hydrostatic pressure of 12 MPa in manifold fed-batch incubation in the HP-MI system yielded a sixfold increase in the volumetric AOM rate. Over subsequent incubation periods AOM rates increased from 0.6 to 1.2 mmol L−1 day−1 within 26 days of incubation. No inhibition of biomass activity was observed in all continuous and fed-batch incubation experiments. The organisms were able to tolerate high sulfide concentrations and extended starvation periods.

Description: Iron is a key micronutrient for phytoplankton growth in the surface ocean. Yet the significance of volcanism for the marine biogeochemical iron-cycle is poorly constrained. Recent studies, however, suggest that offshore deposition of airborne ash from volcanic eruptions is a way to inject significant amounts of bio-available iron into the surface ocean. Volcanic ash may be transported up to several tens of kilometers high into the atmosphere during large-scale eruptions and fine ash may stay aloft for days to weeks, thereby reaching even the remotest and most iron-starved oceanic regions. Scientific ocean drilling demonstrates that volcanic ash layers and dispersed ash particles are frequently found in marine sediments and that therefore volcanic ash deposition and iron-injection into the oceans took place throughout much of the Earth's history. Natural evidence and the data now available from geochemical and biological experiments and satellite techniques suggest that volcanic ash is a so far underestimated source for iron in the surface ocean, possibly of similar importance as aeolian dust. Here we summarise the development of and the knowledge in this fairly young research field. The paper covers a wide range of chemical and biological issues and we make recommendations for future directions in these areas. The review paper may thus be helpful to improve our understanding of the role of volcanic ash for the marine biogeochemical iron-cycle, marine primary productivity and the ocean-atmosphere exchange of CO2 and other gases relevant for climate in the Earth's history.

Description: Epsilonproteobacteria have been found globally distributed in marine anoxic/sulfidic areas mediating relevant transformations within the sulfur and nitrogen cycles. In the Baltic Sea redox zones, chemoautotrophic epsilonproteobacteria mainly belong to the Sulfurimonas gotlandica GD17 cluster for which recently a representative strain, S. gotlandica GD1T, could be established as a model organism. In this study, the potential effects of changes in dissolved inorganic carbon (DIC) and pH on S. gotlandica GD1T were examined. Bacterial cell abundance within a broad range of DIC concentrations and pH values were monitored and substrate utilization was determined. The results showed that the DIC saturation concentration for achieving maximal cell numbers was already reached at 800 μmol L−1, which is well below in situ DIC levels. The pH optimum was between 6.6 and 8.0. Within a pH range of 6.6–7.1 there was no significant difference in substrate utilization; however, at lower pH values maximum cell abundance decreased sharply and cell-specific substrate consumption increased.

Description: Dedicated to the memory of our colleague Klaus Hochheim, who tragically lost his life in the Arctic expedition in September 2013. A distinct, subsurface density front along the eastern St. Anna Trough in the northern Kara Sea is inferred from hydrographic observations in 1996 and 2008–2010. Direct velocity measurements show a persistent northward subsurface current (~ 18 cm s−1) along the St. Anna Trough eastern flank. This sheared flow, carrying the outflow from the Barents and Kara seas to the Arctic Ocean, is also evident from shipboard observations as well as from geostrophic velocities and numerical model simulations. Although we cannot substantiate our conclusions by direct observation-based estimates of mixing rates in the area, we hypothesize that the enhanced vertical mixing along the St. Anna Trough eastern flank favors the upward heat loss from the intermediate warm Atlantic water layer. Modeling results support this hypothesis. The upward heat flux inferred from hydrographic data and model simulations is of O(30–100) W m−2. The region of lowered sea ice thickness and concentration seen both in sea ice remote sensing observations and model simulations marks the Atlantic water pathway in the St. Anna Trough and adjacent Nansen Basin continental margin. In fact, the sea ice shows a delayed freeze-up onset during fall and a reduction in the sea ice thickness during winter. This is consistent with our results on the enhanced Atlantic water heat loss along the Atlantic water pathway in the St. Anna Trough.

Description: Following the launch of ESA's Soil Moisture and Ocean Salinity (SMOS) mission, it has been shown that brightness temperatures at a low microwave frequency of 1.4 GHz (L-band) are sensitive to sea ice properties. In the first demonstration study, sea ice thickness up to 50 cm has been derived using a semi-empirical algorithm with constant tie-points. Here, we introduce a novel iterative retrieval algorithm that is based on a thermodynamic sea ice model and a three-layer radiative transfer model, which explicitly takes variations of ice temperature and ice salinity into account. In addition, ice thickness variations within the SMOS spatial resolution are considered through a statistical thickness distribution function derived from high-resolution ice thickness measurements from NASA's Operation IceBridge campaign. This new algorithm has been used for the continuous operational production of a SMOS-based sea ice thickness data set from 2010 on. The data set is compared to and validated with estimates from assimilation systems, remote sensing data, and airborne electromagnetic sounding data. The comparisons show that the new retrieval algorithm has a considerably better agreement with the validation data and delivers a more realistic Arctic-wide ice thickness distribution than the algorithm used in the previous study (Kaleschke et al., 2012).

Description: Samoylov Island is centrally located within the Lena River Delta at 72° N, 126° E and lies within the Siberian zone of continuous permafrost. The landscape on Samoylov Island consists mainly of late Holocene river terraces with polygonal tundra, ponds and lakes, and an active floodplain. The island has been the focus of numerous multidisciplinary studies since 1993, which have focused on climate, land cover, ecology, hydrology, permafrost and limnology. This paper aims to provide a framework for future studies by describing the characteristics of the island's meteorological parameters (temperature, radiation and snow cover), soil temperature, and soil moisture. The land surface characteristics have been described using high resolution aerial images in combination with data from ground-based observations. Of note is that deeper permafrost temperatures have increased between 0.3 to 1.3 °C over the last five years. However, no clear warming of air and active layer temperatures is detected since 1998, though winter air temperatures during recent years have not been as cold as in earlier years.

Description: Harmful, bloom-forming cyanobacteria (CyanoHABs) are occurring with increasing regularity in freshwater and marine ecosystems. The most commonly occurring cyanobacterial toxins are the hepatotoxic microcystin and nodularin. These cyclic hepta- and pentapeptides are synthesised nonribosomally by the gene products of the toxin gene clusters mcy and nda, respectively. Understanding of the regulation of hepatotoxin production is incomplete, although there is strong evidence supporting the roles of iron, light, higher nitrate availability and inorganic carbon in modulating microcystin levels. The majority of these studies have focused on the unicellular freshwater, microcystin-producing strain of Microcystis aeruginosa, with little attention being paid to terrestrial or marine toxin producers. This review intends to investigate the regulation of microcystin and nodularin production in unicellular and filamentous diazotrophic cyanobacteria against the background of changing climate conditions. Special focus is given to diazotrophic filamentous cyanobacteria, for example Nodularia spumigena, capable of regulating their nitrogen levels by actively fixing dinitrogen. By combining data from significant studies, an overall scheme of the regulation of toxin production is presented, focussing specifically on nodularin production in diazotrophs against the background of increasing carbon dioxide concentrations and temperatures envisaged under current climate change models. Furthermore, the risk of sustaining and spreading CyanoHABs in the future ocean is evaluated.

Description: The first long-term aerosol sampling and chemical characterization results from measurements at the Cape Verde Atmospheric Observatory (CVAO) on the island of São Vicente are presented and are discussed with respect to air mass origin and seasonal trends. In total 671 samples were collected using a high-volume PM10 sampler on quartz fiber filters from January 2007 to December 2011. The samples were analyzed for their aerosol chemical composition, including their ionic and organic constituents. Back trajectory analyses showed that the aerosol at CVAO was strongly influenced by emissions from Europe and Africa, with the latter often responsible for high mineral dust loading. Sea salt and mineral dust dominated the aerosol mass and made up in total about 80% of the aerosol mass. The 5-year PM10 mean was 47.1 ± 55.5 μg m−2, while the mineral dust and sea salt means were 27.9 ± 48.7 and 11.1 ± 5.5 μg m−2, respectively. Non-sea-salt (nss) sulfate made up 62% of the total sulfate and originated from both long-range transport from Africa or Europe and marine sources. Strong seasonal variation was observed for the aerosol components. While nitrate showed no clear seasonal variation with an annual mean of 1.1 ± 0.6 μg m−3, the aerosol mass, OC (organic carbon) and EC (elemental carbon), showed strong winter maxima due to strong influence of African air mass inflow. Additionally during summer, elevated concentrations of OM were observed originating from marine emissions. A summer maximum was observed for non-sea-salt sulfate and was connected to periods when air mass inflow was predominantly of marine origin, indicating that marine biogenic emissions were a significant source. Ammonium showed a distinct maximum in spring and coincided with ocean surface water chlorophyll a concentrations. Good correlations were also observed between nss-sulfate and oxalate during the summer and winter seasons, indicating a likely photochemical in-cloud processing of the marine and anthropogenic precursors of these species. High temporal variability was observed in both chloride and bromide depletion, differing significantly within the seasons, air mass history and Saharan dust concentration. Chloride (bromide) depletion varied from 8.8 ± 8.5% (62 ± 42%) in Saharan-dust-dominated air mass to 30 \textpm 12% (87 ± 11%) in polluted Europe air masses. During summer, bromide depletion often reached 100% in marine as well as in polluted continental samples. In addition to the influence of the aerosol acidic components, photochemistry was one of the main drivers of halogenide depletion during the summer; while during dust events, displacement reaction with nitric acid was found to be the dominant mechanism. Positive matrix factorization (PMF) analysis identified three major aerosol sources: sea salt, aged sea salt and long-range transport. The ionic budget was dominated by the first two of these factors, while the long-range transport factor could only account for about 14% of the total observed ionic mass.

Description: The two stocks of cod (Gadus morhua L.) in the Baltic Sea are separated by two main spawning areas and main spawning periods. Migrations of juveniles and adults result in the mixing of both stocks. At present, the two stocks have been discriminated using inter alia meristics and morphometry, genetics and tagging experiments. However, using these techniques it is not possible to assign single, non-spawning individuals to a certain stock. Since otolith shape analysis has become a common method for stock discrimination and has been applied successfully to several Atlantic cod stocks, it was applied here for the Baltic cod stocks. To obtain baseline samples, spawning cod were sampled between 2005 and 2009 in the main spawning areas during the spawning periods. The otolith contours were digitized and elliptical Fourier descriptors were analyzed. Both stocks differed significantly in their otolith shapes, and individuals could be allocated with 〉90% accuracy to one of the stocks. Both Baltic cod stocks and cod from West Greenland were discriminated with 93% correct classification. These results highlight otolith shape analysis as a useful tool to discriminate adults of Baltic cod stocks.

Description: Climate and weather extremes are sporadically recurring events that may have major local or regional impacts on the society and the environment. These events are typically related to unusually high or low temperature, prolonged dry or wet conditions, heavy precipitation, or extreme winds. Extreme events are part of the overall climate and weather alongside average conditions and variability, and thus are not unexpected as such. Climate change is expected to affect not only means but also variability and extremes. Some inferences can be based on past and present observations, but analyses of especially rare events are hampered by the availability of long time series. Over time, depending on how far the on-going global warming takes us from the present and the past climate conditions, the weather and climate statistics may well come to shift in ways that are well outside observational data. This may lead to shifts in frequency, intensity and geographical distribution of different extremes. Indeed, projected changes in some extremes over the 21st century are quite robust, such as generally increasing warm and decreasing cold extremes. Possible changes in some other aspects, for example storms, remain much more uncertain. Science-based information both on robust findings and on relevant uncertainties on changing extremes can provide useful information for sectorial planning, disaster risk prevention and overall reduction of societal vulnerability related to climate and weather

Description: An earth system model of intermediate complexity (CLIMate and BiosphERe – CLIMBER-2) and a land surface model (JSBACH), which dynamically represent vegetation, are used to simulate natural fire dynamics through the last 8000 yr. Output variables of the fire model (burned area and fire carbon emissions) are used to compare model results with sediment-based charcoal reconstructions. Several approaches for processing model output are also tested. Charcoal data are reported in Z-scores with a base period of 8000–200 BP in order to exclude the strong anthropogenic forcing of fire during the last two centuries. The model–data comparison reveals a robust correspondence in fire activity for most regions considered, while for a few regions, such as Europe, simulated and observed fire histories show different trends. The difference between modelled and observed fire activity may be due to the absence of anthropogenic forcing (e.g. human ignitions and suppression) in the model simulations, and also due to limitations inherent to modelling fire dynamics. The use of spatial averaging (or Z-score processing) of model output did not change the directions of the trends. However, Z-score-transformed model output resulted in higher rank correlations with the charcoal Z-scores in most regions. Therefore, while both metrics are useful, processing model output as Z-scores is preferable to areal averaging when comparing model results to transformed charcoal records.

Description: In this overview, we consider the rationale and practice of invertebrate introductions into Lithuanian waters, the current distribution of introduced species, test the theoretical expectations of introduction of peracaridan species into lakes, and summarize the large-scale consequences of these translocations. After some early attempts to transfer locally available species within the country, peracaridan species from the Ponto-Caspian region were introduced into Lithuanian waters during the 1960s (Pontogammarus robustoides, Obesogammarus crassus, Chaetogammarus warpachowskyi, Paramysis lacustris, Limnomysis benedeni and Hemimysis anomala). After their establishment at the site of first introduction in a newly constructed water reservoir on the Nemunas River, further deliberate introductions into Lithuanian lakes and water reservoirs, as well as into more distant areas, were undertaken. These introduced species soon contributed to fish diet and a subsequent increase in the production of commercially important fish was envisaged. However, our collation of available data does not support the enhancement of fish production in Lithuanian lakes. Although perch (selected as a model fish species) assimilated the introduced species into diet and sometimes in large quantities, there was no subsequent influence on somatic growth rates when compared with perch from lakes devoid of alien peracaridans. Comparisons of littoral fish communities of different lakes, and commercial catches in the lake with the most numerous populations of introduced species also indicate no significant effect on fish production. Negative consequences of introduction are more than obvious in contrast. To date, most of the introduced Ponto-Caspian amphipod and mysid species occur in different sites of the Baltic Sea basin outside Lithuania, and their further dispersal may be expected. These species, especially amphipods, have proved to be highly ecologically aggressive, inducing change in resident macroinvertebrate communities and causing increase of biological contamination (i.e. detract from naturalness). Thus, the introduction of alien fodder species was ill advised from an economic, and even adverse from an environmental perspective.

Description: 1. Macroecology has prospered in recent years due in part to the wide array of climatic data, such as those provided by theWorldClim and CliMond data sets, which has become available for research. However, important environmental variables have still been missing, including spatial data sets on UV-B radiation, an increasingly recognized driver of ecological processes. 2. We developed a set of globalUV-B surfaces (glUV) suitable to match common spatial scales in macroecology. Our data set is based on remotely sensed records fromNASA’sOzone Monitoring Instrument (Aura-OMI). Following a similar approach as for theWorldClim and CliMond data sets, we processed daily UV-B measurements acquired over a period of eight years into monthly mean UV-B data and six ecologically meaningful UV-B variables with a 15-arc minute resolution. These bioclimatic variables represent Annual Mean UV-B, UV-B Seasonality, Mean UV-B of Highest Month, Mean UV-B of Lowest Month, Sum of Monthly Mean UV-B during Highest Quarter and Sum of Monthly Mean UV-B during Lowest Quarter. We correlated our data sets with selected variables of existing bioclimatic surfaces for land and with Terra–MODIS Sea Surface Temperature for ocean regions to test for relations to known gradients and patterns. 3. UV-B surfaces showed a distinct seasonal variance at a global scale, while the intensity of UV-B radiation decreased towards higher latitudes and was modified by topographic and climatic heterogeneity. UV-B surfaces were correlated with global mean temperature and annual mean radiation data, but exhibited variable spatial associations across the globe.UV-B surfaces were otherwise widely independent of existing bioclimatic surfaces. 4. Our data set provides new climatological information relevant for macroecological analyses. As UV-B is a known driver of numerous biological patterns and processes, our data set offers the potential to generate a better understanding of these dynamics inmacroecology, biogeography, global change research and beyond. The glUV data set containing monthly mean UV-B data and six derived UV-B surfaces is freely available for download at: http://www.ufz.de/gluv.

Description: The tropospheric response to sudden stratospheric warmings (SSWs) is analyzed in an idealized model setup regarding the respective roles of planetary-scale and synoptic-scale waves. The control model run includes a full interactive wave spectrum, while a second run includes interactive planetary-scale waves but only the time-mean synoptic-scale wave forcing from the control run. In both runs, the tropospheric response is characterized by the negative phase of the respective tropospheric annular mode. But given their different latitudinal structure, the control run shows the expected response, i.e., an equatorward shift of the tropospheric jet, whereas the response in the absence of interactive synoptic eddies is characterized by a poleward jet shift. This opposite jet shift is associated with a different planetary wave variability that couples with the zonal flow between the stratosphere and the surface. These results indicate that the synoptic eddy feedback is necessary for the observed tropospheric response to SSWs.

Description: Natural antifouling products have been the subject of considerable attention. We screened marine algae for antifouling activity and discovered omaezallenes, the new bromoallene-containing natural products isolated from the red alga Laurencia sp. Described is the isolation, structure elucidation, and total syntheses of omaezallenes. The relative and absolute configurations of natural omaezallenes were unambiguously established through total synthesis. The antifouling activities and ecotoxicity of omaezallenes were also evaluated.