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  • 1
    Publikationsdatum: 2021-06-16
    Beschreibung: In this study, Mg/Ca, Sr/Ca and Ba/Ca ratios in a Lateglacial to Holocene stalagmite (CC26) from Corchia Cave (central Italy) are compared with stable isotope data to define palaeohydrological changes. For most of the record, the trace element ratios show small absolute variability but similar patterns, which are also consistent with stable isotope variations. Higher trace element-to-calcium values are interpreted as responses to decreasing moisture, inducing changes in the residence time of percolation, producing prior calcite precipitation and/or variations in the hydrological routing. Statistically meaningful levels of covariability were determined using anomalies of Mg/Ca, d18O and d13C. Combining these three time series into a single ‘palaeomoisture-trend’ parameter, we highlight several events of reduced moisture (ca. 8.9–8.4, 6.2, 4.2, 3.1 and 2.0 ka), a humid period between ca. 7.9 and 8.3 ka and other shorter-term wet events at ca. 5.8, 5.3 and 3.7 ka. Most of these events can be correlated with climate changes inferred from other regional studies. For both extremities of the record (i.e. before ca. 12.4 ka and after ca. 0.5 ka) Mg/Ca and Sr/Ca are anti-correlated and show the greatest amplitude of values, a likely explanation for which involves aragonite and/or gypsum precipitation (the latter derived from pyrite oxidation) above the CC26 drip point.
    Beschreibung: Published
    Beschreibung: 381–392
    Beschreibung: 4A. Clima e Oceani
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): central Italy; Corchia Cave; Holocene; speleothems; trace elements ; 01. Atmosphere::01.01. Atmosphere::01.01.02. Climate
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2021-06-14
    Beschreibung: This study is focused on the (micro)biogeochemical features of two close geothermal sites (FAV1 and FAV2), both selected at the main exhalative area of Pantelleria Island, Italy. A previous biogeochemical survey revealed high CH4 consumption and the presence of a diverse community of methanotrophs at FAV2 site, whereas the close site FAV1 was apparently devoid of methanotrophs and recorded no CH4 consumption. Next-Generation Sequencing (NGS) techniques were applied to describe the bacterial and archaeal communities which have been linked to the physicochemical conditions and the geothermal sources of energy available at the two sites. Both sites are dominated by Bacteria and host a negligible component of ammonia-oxidizing Archaea (phylum Thaumarchaeota). The FAV2 bacterial community is characterized by an extraordinary diversity of methanotrophs, with 40% of the sequences assigned to Methylocaldum, Methylobacter (Gammaproteobacteria) and Bejerickia (Alphaproteobacteria); conversely, a community of thermo-acidophilic chemolithotrophs (Acidithiobacillus, Nitrosococcus) or putative chemolithotrophs (Ktedonobacter) dominates the FAV1 community, in the absence of methanotrophs. Since physical andchemical factors of FAV1, such as temperature and pH, cannot be considered limiting for methanotrophy, it is hypothesized that the main limiting factor for methanotrophs could be high NH4+ concentration. At the same time, abundant availability of NH4+ and other high energy electron donors and acceptors determined by the hydrothermal flux in this site create more energetically favourable conditions for chemolithotrophs that outcompete methanotrophs in non-nitrogen-limited soils.
    Beschreibung: Published
    Beschreibung: 150–162
    Beschreibung: 4V. Vulcani e ambiente
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): geothermal soils ; geomicrobiology ; chemolithotrophs ; methanotrophs ; Pantelleria ; 04. Solid Earth::04.04. Geology::04.04.12. Fluid Geochemistry
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Publikationsdatum: 2021-03-18
    Beschreibung: This article presents an integrated approach for the probabilistic systemic risk analysis of a road network considering spatial seismic hazard with correlation of ground motion intensities, vulnerability of the network components, and the effect of interactions within the network, as well as, between roadway components and built environment to the network functionality. The system performance is evaluated at the system level through a global connectivity performance indicator, which depends on both physical damages to its components and induced functionality losses due to interactions with other systems. An object-oriented modeling paradigm is used, where the complex problem of several interacting systems is decomposed in a number of interacting objects, accounting for intra- and interdependencies between and within systems. Each system is specified with its components, solving algorithms, performance indicators and interactions with other systems. The proposed approach is implemented for the analysis of the road network in the city of Thessaloniki (Greece) to demonstrate its applicability. In particular, the risk for the road network in the area is calculated, specifically focusing on the short-term impact of seismic events (just after the earthquake). The potential of road blockages due to collapses of adjacent buildings and overpass bridges is analyzed, trying to individuate possible criticalities related to specific components/subsystems. The application can be extended based on the proposed approach, to account for other interactions such as failure of pipelines beneath the road segments, collapse of adjacent electric poles, or malfunction of lighting and signaling systems due to damage in the electric power network.
    Beschreibung: Published
    Beschreibung: 524–540
    Beschreibung: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Systemic vulnerability ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Publikationsdatum: 2020-12-14
    Beschreibung: The knowledge of the local soil structure is important for the assessment of seismic hazards. A widespread, but time-consuming technique to retrieve the parameters of the local underground is the drilling of boreholes. Another way to obtain the shear wave velocity profile at a given location is the inversion of surface wave dispersion curves. To ensure a good resolution for both superficial and deeper layers, the used dispersion curves need to cover a wide frequency range. This wide frequency range can be obtained using several arrays of seismic sensors or a single array comprising a large number of sensors. Consequently, these measurements are time-consuming. A simpler alternative is provided by the use of the ellipticity of Rayleigh waves. The frequency dependence of the ellipticity is tightly linked to the shear wave velocity profile. Furthermore, it can be measured using a single seismic sensor. As soil structures obtained by scaling of a given model exhibit the same ellipticity curve, any inversion of the ellipticity curve alone will be ambiguous. Therefore, additional measurements which fix the absolute value of the shear wave velocity profile at some points have to be included in the inversion process. Small-scale spatial autocorrelation measurements or MASW measurements can provide the needed data. Using a theoretical soil structure, we show which parts of the ellipticity curve have to be included in the inversion process to get a reliable result and which parts can be omitted. Furthermore, the use of autocorrelation or high-frequency dispersion curves will be highlighted. The resulting guidelines for inversions including ellipticity data are then applied to real data measurements collected at 14 different sites during the European NERIES project. It is found that the results are in good agreement with dispersion curve measurements. Furthermore, the method can help in identifying the mode of Rayleigh waves in dispersion curve measurements.
    Beschreibung: Published
    Beschreibung: 207-229
    Beschreibung: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Inverse theory Surface waves and free oscillations Site effects Computational seismology Wave propagation ; 04. Solid Earth::04.02. Exploration geophysics::04.02.06. Seismic methods
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Publikationsdatum: 2021-04-07
    Beschreibung: Stable isotopes were measured in the carbonate and organic matter of palaeosols in the Somma–Vesuvius area, southern Italy in order to test whether they are suitable proxy records for climatic and ecological changes in this area during the past 18000 yr. The ages of the soils span from ca. 18 to ca. 3 kyr BP. Surprisingly, the Last Glacial to Holocene climate transition was not accompanied by significant change in d18O of pedogenic carbonate. This could be explained by changes in evaporation rate and in isotope fractionation between water and precipitated carbonate with temperature, which counterbalanced the expected change in isotope composition of meteoric water. Because of the rise in temperature and humidity and the progressive increase in tree cover during the Holocene, the Holocene soil carbonates closely reflect the isotopic composition of meteoric water. A cooling of about 2°C after the Avellino eruption (3.8 ka) accounts for a sudden decrease of about 1‰ in d18O of pedogenic carbonate recorded after this eruption. The d13C values of organic matter and pedogenic carbonate covary, indicating an effective isotope equilibrium between the organic matter, as the source of CO2, and the pedogenic carbonate. Carbon isotopes suggest prevailing C3 vegetation and negligible mixing with volcanogenic or atmospheric CO2.
    Beschreibung: Published
    Beschreibung: 813-824
    Beschreibung: 1V. Storia e struttura dei sistemi vulcanici
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): stable isotope ; palaeosols ; Somma–Vesuvius ; palaeoclimate ; 04. Solid Earth::04.08. Volcanology::04.08.99. General or miscellaneous
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Publikationsdatum: 2021-06-22
    Beschreibung: Operative seismic aftershock risk forecasting can be particularly useful for rapid decision-making in the presence of an ongoing sequence. In such a context, limit state first-excursion probabilities (risk) for the forecasting interval (a day) can represent the potential for progressive state of damage in a structure. This work lays out a performance-based framework for adaptive aftershock risk assessment in the immediate post-mainshock environment. A time-dependent structural performance variable is adopted in order to measure the cumulative damage in a structure. A set of event-dependent fragility curves as a function of the first-mode spectral acceleration for a prescribed limit state is calculated by employing back-to-back non- linear dynamic analyses. An epidemic-type aftershock sequence model is employed for estimating the spatio-temporal evolution of aftershocks. The event-dependent fragility curves for a given limit state are then integrated together with the probability distribution of aftershock spectral acceleration based on the epidemic-type aftershock sequence aftershock hazard. The daily probability of limit state first-excursion is finally calculated as a weighted combination of the sequence of limit state probabilities conditioned on the num- ber of aftershocks. As a numerical example, daily aftershock risk is calculated for the L’Aquila 2009 aftershock sequence (central Italy). A representative three-story reinforced concrete frame with infill panels, which has cyclic strength and stiffness degradation, is used in order to evaluate the progressive damage. It is observed that the proposed framework leads to a sound forecasting of limit state first-excursion in the structure for two limit states of significant damage and near collapse. Copyright © 2014 John Wiley & Sons, Ltd.
    Beschreibung: Published
    Beschreibung: 2179–2197
    Beschreibung: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Beschreibung: JCR Journal
    Beschreibung: reserved
    Schlagwort(e): aftershock ; time-dependent reliability ; seismic risk ; etas modeling ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    facet.materialart.
    Unbekannt
    Wiley-Blackwell
    In:  EPIC3Harmful Algal Blooms: A Compendium Desk Reference, Wiley-Blackwell, 12 p., pp. 563-574, ISBN: 978-1-118-99465-8
    Publikationsdatum: 2018-06-28
    Beschreibung: The genus Alexandrium (Halim) is perhaps the most intensively studied among toxic marine dinoflagellates. This is largely attributable to the devastating consequences of toxigenic blooms of this genus, with human poisonings from contaminated seafood, primarily from shellfish and more rarely from finfish; socio–economic losses to the aquaculture and fisheries industries; marine faunal mortalities; and food web disruptions common in coastal waters throughout the world. Members of this genus are globally distributed from the Arctic to the tropics, and in both hemispheres from sub–polar through temperate to sub–tropical to tropicalwaters. At least four distinct groups of marine phycotoxins are associated with various Alexandrium species, along with poorly characterized bioactive compounds (allelochemicals) that may affect species interactions among the plankton. According to the most recent iteration of the IOC–UNESCO reference list of toxic microalgae, there are now more than 30 recognized morphological species of Alexandrium, posing a daunting challenge for risk assessment and accurate identification in toxic phytoplankton monitoring programs.
    Repository-Name: EPIC Alfred Wegener Institut
    Materialart: Inbook , peerRev
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  • 8
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    Unbekannt
    Wiley-Blackwell
    In:  EPIC3Climate Change Impacts on Fisheries and Aquaculture: A Global Analysis, Climate Change Impacts on Fisheries and Aquaculture: A Global Analysis, Wiley-Blackwell, pp. 663-701, ISBN: 978-1-119-15404-4
    Publikationsdatum: 2017-10-09
    Beschreibung: Exploitation of Southern Ocean marine resources began more than 200 years ago with the massive hunt for seals and whales. In the 1960s/70s, fisheries for finfish and krill entered Southern Ocean waters. Within a few years many fish populations were heavily overfished and dramatically depleted, and some of these stocks still did not recover. Today, fish stocks and fisheries activities are managed and monitored by the Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR) which was established in 1982 to ensure sustainable exploitation and protection of the delicate marine ecosystem. Current target species include Mackerel icefish (Champsocephalus gunnari), Patagonian as well as Antarctic toothfish (Dissostichus eleginoides and D. mawsoni) and Antarctic krill (Euphausia superba). Most of these species are vulnerable to overfishing due to slow growth, late age at maturity, and rather low fecundity. This vulnerability might increase, as Southern Ocean living communities are currently also faced with alterations of their environment due to climate change, such as increasing water temperatures and decreasing sea ice. Species, including the ones targetted by fisheries, are well-adapted to their particular environmental conditions and are believed to be highly sensitive to changes because of their cold-adapted physiology, their life history traits, and their direct or indirect dependence on sea ice. The species will be exposed to several stressors at the same time, and fishing pressure, direct abiotic forcing and changes mediated via the food web might act synergistically and result in significant population declines. In particular the strongly sea ice-dependent Antarctic krill, a key species in the food web, might be adversely affected. Fish species seems to have low tolerance towards higher water temperatures and may thus, in the long run, be replaced by lower latitude species.
    Repository-Name: EPIC Alfred Wegener Institut
    Materialart: Inbook , peerRev
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    facet.materialart.
    Unbekannt
    Wiley-Blackwell
    In:  EPIC3Harmful Algal Blooms: A Compendium Desk Reference, Wiley-Blackwell, 8 p., pp. 605-612, ISBN: 978-1-118-99465-8
    Publikationsdatum: 2018-06-28
    Repository-Name: EPIC Alfred Wegener Institut
    Materialart: Inbook , peerRev
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  • 10
    Publikationsdatum: 2017-04-04
    Beschreibung: The Pernicana Fault (PF) is the main structural element of Mt Etna and the northern boundary of a section sliding to the southeast. Observed ground motion records in the damage zone of the PF show strong variations of directional resonance in the horizontal plane. The observed resonance directions exhibit an abrupt rotation of azimuth by about 30◦ across the fault, varying from N166◦ on the north side to N139◦ on the south. We interpret the directional resonance observations in terms of changes in the kinematics and deformation fields on the opposite sides of the fault. The northern side is affected primarily by the left-lateral strike-slip movement, whereas the southern side, that is subjected also to sliding, is under a dominant extensional stress regime. Brittle deformation models based on the observed kinematic field predict different sets of fractures on the opposite sides of the fault: synthetic cleavages and extensional fractures are expected to dominate in the northern and southern sides, respectively. These two fracture fields have different orientations (N74◦ and N42◦, respectively) and both show a near-orthogonal relation (∼88◦ in the northern sector and ∼83◦ to the south) with the azimuth of the observed directional resonance. We conclude that the direction of the largest resonance motions is sensitive to and has transversal relationship with the dominant fracture orientation. The directional amplification is inferred to be produced by stiffness anisotropy of the fault damage zone, with larger seismic motions normal to the fractures.
    Beschreibung: Published
    Beschreibung: 986–996
    Beschreibung: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Earthquake ground motions; Site effects; Wave propagation ; 04. Solid Earth::04.06. Seismology::04.06.09. Waves and wave analysis
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 11
    facet.materialart.
    Unbekannt
    Wiley-Blackwell
    Publikationsdatum: 2017-04-04
    Beschreibung: We consider a seismicity forecast experiment conducted during the last 4 yr. At the beginning of each year, three models make a 1-yr forecast of the distribution of large earthquakes everywhere on the Earth. The forecasts are generated and the observations are collected in the Collaboratory for the Study of Earthquake Predictability (CSEP). We apply CSEP likelihood measures of consistency and comparison to see how well the forecasts match the observations, and we compare results from some intuitive reference models. These results illustrate some undesirable properties of the consistency tests: the tests can be extremely sensitive to only a few earthquakes, and yet insensitive to seemingly obvious flaws—a na ̈ıve hypothesis that large earthquakes are equally likely everywhere is not always rejected. The results also suggest that one should check the assumptions of the so-called T and W comparison tests, and we illustrate some methods to do so. As an extension of model assessment, we explore strategies to combine forecasts, and we discuss the implications for operational earthquake forecasting. Finally, we make suggestions for the next generation of global seismicity forecast experiments.
    Beschreibung: Published
    Beschreibung: 422-431
    Beschreibung: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): probabilistic forecasting ; statistical seismology ; 04. Solid Earth::04.06. Seismology::04.06.02. Earthquake interactions and probability
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 12
    Publikationsdatum: 2017-04-04
    Beschreibung: The M ∼ 7 1915 Fucino (Central Italy) earthquake represents one of the most destructive seismic events ever occurred in the Italian Peninsula. Several seismogenic faults have been proposed in the past decades as the source of the earthquake by means of different approaches and techniques that lead to a variety of speculations about the source mechanism and the fault location, often contrasting with one another. The 1915 earthquake produced a remarkable data set of 73 coseismic hydrological changes in the near and intermediate field that consist in variation of the flow of streams and springs, liquefaction, rise of water temperature and turbidity. In this paper, we study the coseismic water level changes induced by the 1915 earthquake in the near field to provide convincing clues on the geometry of the earthquake causative fault. We model the coseismic strain field induced by seventeen individual faults proposed through different approaches, and compare its pattern with the distribution of streamflow changes. We find: (i) clues on the most probable geometry of the earthquake causative fault. Best fits between modelled deformation and observed data are displayed by sources (derived by geological or seismological data) that share several distinctive features, as they are ∼135◦-striking, SW-dipping, 25–30-km-long normal faults located along the eastern side of the Fucino basin. These data point to the Serrone Fault and the Parasano Fault as the most likely causative structures and support the hypothesis that the coseismic ruptures observed in the field represented primary surface faulting. On the contrary, our calculations show that the Pescina Fault and the Ventrino Fault are secondary faults from the perspective of the hydrological response. Finally, one of the best scoring potential sources (from geological data) is a multifaulting system that considers the presence, in the central-western part of the basin, of fault splays synthetic and antithetic to the main seismogenic structures; therefore, we infer for these splays a possible active involvement in a 1915-like seismogenic process; (ii) evidence against a number of seismogenic structures that were previously associated with the earthquake. In particular, the plots of coseismic strain induced by sources uniquely derived by macroseismic or geodetic data prove to be inconsistent with the polarities of the hydrological signatures. Also, sources mainly characterized by reverse faulting and/or by right-lateral strike-slip component are discarded and (iii) as a final remark, we maintain that the study of the hydrological signatures of earthquake strain can offer an alternative tool in the investigation of the historical seismicity, to estimate the focal mechanism of major earthquakes capable of giving rise to a consistent data set of hydrological data.
    Beschreibung: Published
    Beschreibung: 1374-1388
    Beschreibung: 2T. Tettonica attiva
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): 1915 Fucino earthquake ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 13
    Publikationsdatum: 2017-04-04
    Beschreibung: Macroseismic intensities are the only available data for most historical earthquakes and often represent the unique source of information for crucial events in the definition of seismic hazard. In this paper, we attempt at getting insight into source characteristics by reproducing the observed intensity field. As a test case, we study the source of 1908 Messina Straits earthquake ( M W = 7.1), by testing three distinct fault models deduced from the analysis of geodeticdata.Startingfromthestaticslipdistribution,wedevelop kinematicsourcemodelsfor the investigated fault and compute full waveform synthetic seismograms in a 1-D structural model, also accounting for anelastic attenuation. Then, we convert both computed peak- ground acceleration (PGA) and peak-ground velocity (PGV) to macroseismic intensity at 100 selected sites, by means of specific empirical relations for the Italian region. By comparing the original data separately with PGA- and PGV-based intensity fields, we discriminate among the tested faults and determine the best values for the investigated kinematic parameters of the source. We also perform a misfit analysis for the best source model, in order to investigate the dependence of the results on the selected parametrization. The results of the analysis indicate that among the tested models, the one characterized by an east-dipping fault, with strike- oriented NS slightly rotated clockwise, better explains the observed macroseismic field of the 1908 Messina Straits earthquake. Besides, the fracture nucleated at the southern end of the fault and ruptured northward, producing considerable directivity effects. This is in agreement with the published results obtained from the investigation of the historical seismograms. We alsodeterminerealisticvalues fortherupturevelocityand therise-time.Ourstudyconfirms the greatpotentialofthemacroseismicdata,demonstratingthattheycontainenoughinformationto constrain important characteristics of the fault, which can be retrieved by using complex source models and computing complete wavefield. Moreover, we also show that the simultaneous comparison of both PGA- and PGV-based synthetic macroseismic fields with the original intensities provides tighter constraints for discriminating among different source models, with respect to what attainable from each of them
    Beschreibung: Published
    Beschreibung: 164-173
    Beschreibung: 4T. Fisica dei terremoti e scenari cosismici
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Earthquake ground motions; Earthquake source observations. ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 14
    Publikationsdatum: 2017-04-04
    Beschreibung: Gravity and height changes, reflecting magma accumulation in subsurface chambers, are evaluated using Finite Element models in order to resolve controversial relationships observed in some volcanic areas. When significant gravity changes occur without any significant deformation, or vice versa, it is often difficult, if not impossible, to jointly explain the observations using the popular Mogi model. Here we explore whether these discrepancies can be explained by magma compressibility and source geometry effects. Compression of resident magma and expansion of the chamber wall act concurrently to accommodate newly added magma. Gravity-height ratios are found to mainly depend on: (i) geometry of the sources, which control the volume expansion of the chamber, (ii) magma compressibility, which affects the contraction of the magma resident in the chamber, and (iii) depth of the sources. Our numerical results show that, when magma compressibility and non-spherical sources are taken into account, significant gravity variations can, indeed, be successfully reconciled with negligible height changes. This may be the case at Etna volcano, where gravity changes (about 40 miuGal) without any significant deformation (below 5 cm) were observed during the 1994-1995 inflation period. The numerical results point to the accumulation of a 1.4x10^10 kg mass into an elongated source simulating a shallow storage region supplying the summit craters.
    Beschreibung: Published
    Beschreibung: 164-173
    Beschreibung: 4V. Vulcani e ambiente
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): numerical modeling, gravity and height changes ; 04. Solid Earth::04.03. Geodesy::04.03.05. Gravity variations
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 15
    Publikationsdatum: 2017-04-04
    Beschreibung: We present an up-to-date high resolution picture of the ongoing crustal deformation field of Italy, based on an extensive combination of permanent and non-permanent GPS observations carried out since 1994. In addition, we present an updated map of contemporary SHmax orientations computed by a multidisciplinary data set of well-constrained stress indicators, including both published results and novel analyses. The comparison of stress and geodetic strain-rates directions reveals that both patterns are near-parallel over a large part of the investigated area, highlighting that crustal stress and surface deformation are driven by the same mechanism. The comparison of the azimuthal patterns of surface strain and mantle deformation shows a modest correlation on the Alps and a low correlation along the Apennines chain and the Calabro-Peloritan Arc. Along the Apennines chain, this feature suggests the occurrence of significant strain partitioning and crust–mantle mechanical decoupling. Along the Calabro-Peloritan Arc, the apparent low correlation reflects a different mantle–crust mechanism of deformation to the ongoing subduction and rollback of the Ionian slab. In addition, the superposition of regional/local effects related to second-order sources (crustal lateral density changes, strength contrasts), which at regional/local scale modulate the crustal stress/strain-rate pattern, cannot be ruled out.
    Beschreibung: Published
    Beschreibung: 969-985
    Beschreibung: 1T. Geodinamica e interno della Terra
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Plate motions ; Seismic anisotropy ; Kinematics of crustal and mantle deformation ; 04. Solid Earth::04.03. Geodesy::04.03.01. Crustal deformations
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 16
    Publikationsdatum: 2017-04-04
    Beschreibung: In the Umbria Marche (Central Italy) region an important earthquake sequence occurred in 1997, characterized by nine earthquakes with magnitudes in the range between 5 and 6, that caused important damages and causalities. In the present paper we separately estimate intrinsic- and scattering- Q −1 parameters, using the classical MLTWA approach in the assumption of a half space model. The results clearly show that the attenuation parameters Qi −1 and Qs −1 are frequency dependent. This estimate is compared with other attenuation studies carried out in the same area, and with all the other MLTWA estimates obtained till now in other tectonic environments in the Earth. The bias introduced by the half space assumption is investigated through numerical solutions of the Energy Transport equation in the more realistic assumption of a heterogeneous crust overlying a transparent mantle, with a Moho located at a depth ranging between 35 and 45 km below the surface. The bias introduced by the half space assumption is significant only at high frequency. We finally show how the attenuation estimates, calculated with different techniques, lead to different PGA decay with distance relationships, using the well known and well proven Boore’s method. This last result indicates that care must be used in selecting the correct estimate of the attenuation parameters for seismic risk purposes. We also discuss the reason why MLTWA may be chosen among all the other available techniques, due to its intrinsic stability, to obtain the right attenuation parameters.
    Beschreibung: Published
    Beschreibung: 1370-1382
    Beschreibung: 4T. Fisica dei terremoti e scenari cosismici
    Beschreibung: JCR Journal
    Beschreibung: open
    Schlagwort(e): Seismic attenuation ; scattering ; 04. Solid Earth::04.06. Seismology::04.06.09. Waves and wave analysis
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 17
    Publikationsdatum: 2017-04-04
    Beschreibung: Seismological, geological and geodetic data have been integrated to characterize the seismogenic structure of the late 2013-early 2014 moderate energy (maximum local magnitude MLmax = 4.9) seismic sequence that struck the interior of the Matese Massif, part of the Southern Apennines active extensional belt. The sequence, heralded by a ML = 2.7 foreshock, was characterized by two main shocks with ML = 4.9 and ML = 4.2, respectively, which occurred at a depth of ∼17–18 km. The sequence was confined in the 10–20 km depth range, significantly deeper than the 1997–1998 sequence which occurred few km away on the northeastern side of the massif above ∼15 km depth. The depth distribution of the 2013–14 sequence is almost continuous, albeit a deeper (16–19 km) and a shallower (11–15 km) group of events can be distinguished, the former including the main shocks and the foreshock. The epicentral distribution formed a ∼10 km long NNW–SSE trending alignment, which almost parallels the surface trace of late Pliocene–Quaternary southwest-dipping normal faults with a poor evidence of current geological and geodetic deformation. We built an upper crustal model profile for the eastern Matese massif through integration of geological data, oil exploration well logs and seismic tomographic images. Projection of hypocentres on the profile suggests that the seismogenic volume falls mostly within the crystalline crust and subordinately within the Mesozoic sedimentary cover of Apulia, the underthrust foreland of the Southern Apennines fold and thrust belt. Geological data and the regional macroseismic field of the sequence suggest that the southwest-dipping nodal plane of the main shocks represents the rupture surface that we refer to here as the Matese fault. The major lithological discontinuity between crystalline and sedimentary rocks of Apulia likely confined upward the rupture extent of the Matese fault. Repeated coseismic failure represented by the deeper group of events in the sequence, activated in a passive fashion the overlying ∼11–15 km deep section of the upper crustal normal faults. We consider the southwest-dipping Matese fault representative of a poorly known type of seismogenic structures in the Southern Apennines, where extensional seismogenesis and geodetic strain accumulation occur more frequently on NE-dipping, shallower-rooted faults. This is the case of the Boiano Basin fault located on the northern side of the massif, to which the 1997–1998 sequence is related. The close proximity of the two types of seismogenic faults at the Matese Massif is related to the complex crustal architecture generated by the Pliocene–early Pleistocene contractional and transpressional tectonics.
    Beschreibung: Published
    Beschreibung: 823-837
    Beschreibung: 2T. Tettonica attiva
    Beschreibung: JCR Journal
    Beschreibung: partially_open
    Schlagwort(e): Seismicity and tectonics ; Continental tectonics: extensional ; Crustal structure ; 04. Solid Earth::04.04. Geology::04.04.01. Earthquake geology and paleoseismology ; 04. Solid Earth::04.04. Geology::04.04.09. Structural geology ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 18
    Publikationsdatum: 2017-04-04
    Beschreibung: We analysed the conversion problem between teleseismic magnitudes (Ms and mb) provided by the Seismological Bulletin of the International Seismological Centre and moment magni- tudes (Mw) provided by online moment tensor (MT) catalogues using the chi-square general orthogonal regression method (CSQ) that, differently from the ordinary least-square regres- sion method (OLS), accounts for the measurement errors of both the predictor and response variables. To account for the non-linearity of the relationships, we used two types of curvilin- ear models: (i) the exponential model (EXP), recently proposed by the authors of the Global Catalogue sponsored by the Global Earthquake Model (GEM) Foundation and (ii) a connected bilinear (CBL) model, similar to that proposed by Ekstro ̈m & Dziewonski, where two different linear trends at low and high magnitudes are connected by an arc of circle that preserves the continuity of the function and of its first derivative at the connecting points. For Ms, we found that the regression curves computed for a global data set (GBL) are likely to be biased by the incompleteness of global MT catalogues for Mw 〈5.0–5.5. In fact, the GBL curves deviate significantly from a similar regression curve computed for a Euro-Mediterranean data set (MED) integrated with the data provided by two regional MT catalogues including many more events with Mw 〈 5.0–5.5. The GLB regression curves overestimate the Mw proxies computed from Ms up to 0.5 magnitude units. Hence for computing Mw proxies at the global scale of Ms ≤ 5.5, we suggest to adopt the coefficients obtained from the MED regression. The analysis of the frequency–magnitude relationship of the resulting Mw proxy catalogues confirms the validity of this choice as the behaviour of b-value as a function of cut-off magnitude of the GBL data set is much more stable using such approach. The incompleteness of Mw’s provided from MT global catalogues also affects the mb GBL data set but in this case the use of the CSQ regression method, in place of the OLS, mitigates the bias and then, at low magnitudes, the EXP regression curve computed from the more complete MED data set almost coincides with that computed from the GBL data set. Our results also indicate that the slope at low magnitudes of the Mw–Ms relationship is substantially consistent with the hypothesized theoretical value of 2/3 for Ms 〈 5.0 while the slope of the Mw–mb relationship at high magnitudes probably reaches the theoretically expected value of 2 only in the proximity of the upper limit of mb determinations in our data set (mb = 7.2).
    Beschreibung: Published
    Beschreibung: 805–828
    Beschreibung: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Earthquake source observations ; Statistical seismology ; 05. General::05.02. Data dissemination::05.02.02. Seismological data
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 19
    Publikationsdatum: 2017-04-04
    Beschreibung: In a recent paper, important issues were raised about the identification of the fault responsible for the 1908 Messina Straits earthquake. Starting with a reanalysis of the available original geodetic data, the authors aimed to demonstrate that both of the fault–plane orientations derived by the focal mechanism are compatible with the measurements. On these grounds, and based on geological considerations, they argued in favour of the Armo fault—a high-angled structure on the Calabrian side of the Messina Straits—as responsible for the 1908 earthquake. We indicate here that their analysis has some pitfalls that produce questionable results, and that render their conclusions unreliable. Moreover, especially when dealing with such old events and data, we consider that it is more prudent not to derive conclusions on the basis of a single data set, as all of the available information should be included in any interpretation. Indeed, when the joint results of the seismological and geodetic analyses are taken into account, a consistent and robust source model can be derived that indicates that a low-angle, east-dipping fault is the most likely source of this 1908 Messina Straits earthquake
    Beschreibung: Published
    Beschreibung: 1399-1402
    Beschreibung: 4T. Fisica dei terremoti e scenari cosismici
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Earthquake source observations; Seismicity and tectonics; Europe ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 20
    Publikationsdatum: 2017-04-04
    Beschreibung: In this study,we use Differential Synthetic Aperture Radar Interferometry (DInSAR) and multiaperture interferometry (MAI) to constrain the sources of the three largest events of the 2008 Baluchistan (western Pakistan) seismic sequence, namely two Mw 6.4 events only 12 hr apart and an Mw 5.7 event that occurred 40 d later. The sequence took place in the Quetta Syntaxis, the most seismically active region of Baluchistan, tectonically located between the colliding Indian Plate and the Afghan Block of the Eurasian Plate. Surface displacements estimated from ascending and descending ENVISAT ASAR acquisitions were used to derive elastic dislocation models for the sources of the two main events. The estimated slip distributions have peak values of 120 and 130 cm on a pair of almost parallel and near-vertical faults striking NW–SE, and of 50 cm and 60 cm on two high-angle faults striking NE–SW. Values up to 50 cm were found for the largest aftershock on an NE–SW fault located between the sources of the main shocks. The MAI measurements, with their high sensitivity to the north–south motion component, are crucial in this area to accurately describe the coseismic displacement field. Our results provide insight into the deformation style of the Quetta Syntaxis, suggesting that right-lateral slip released at shallow depths on large NW fault planes is compatible with left-lateral activation on smaller NE–SW faults.
    Beschreibung: Published
    Beschreibung: 25-39
    Beschreibung: 2T. Tettonica attiva
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Radar interferometry ; Satellite geodesy ; Seismicity and Tectonics ; Continental margins: convergent ; Earthquake interaction, forecasting and prediction ; Earthquake source observation ; 04. Solid Earth::04.07. Tectonophysics::04.07.07. Tectonics
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 21
    Publikationsdatum: 2017-04-04
    Beschreibung: The relative seismic velocity variations possibly associated to large earthquakes can be readily monitored via cross-correlation of seismic noise. In a recently published study, more than 2 yr of continuous seismic records have been analysed from three stations surrounding the epicentre of the 2009 April 6, Mw 6.1 L’Aquila earthquake, observing a clear decrease of seismic velocities likely corresponding to the co-seismic shaking. Here, we extend the analysis in space, including seismic stations within a radius of 60 km from the main shock epicentre, and in time, collecting 5 yr of data for the six stations within 40 km of it. Our aim is to investigate how far the crustal damage is visible through this technique, and to detect a potential post-seismic recovery of velocity variations. We find that the co-seismic drop in velocity variations extends up to 40 km from the epicentre, with spatial distribution (maximum around the fault and in the north– east direction from it) in agreement with the horizontal co-seismic displacement detected by global positioning system (GPS). In the first few months after L’Aquila earthquake, the crust’s perturbation in terms of velocity variations displays a very unstable behaviour, followed by a slow linear recovery towards pre-earthquake conditions; by almost 4 yr after the event, the co-seismic drop of seismic velocity is not yet fully recovered. The strong oscillations of the velocity changes in the first months after the earthquake prevent to detect the fast exponential recovery seen by GPS data. A test of differently parametrized fitting curves demonstrate that the post-seismic recovery is best explained by a sum of a logarithmic and a linear term, suggesting that processes like viscoelastic relaxation, frictional afterlip and poroelastic rebound may be acting concurrently.
    Beschreibung: Published
    Beschreibung: 604-6011
    Beschreibung: 4T. Fisica dei terremoti e scenari cosismici
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Time-series analysis; Interferometry; Computational seismology; Europe ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 22
    Publikationsdatum: 2019-12-13
    Beschreibung: Downtown L'Aquila suffered severe damage (VIII-IX EMS98 intensity) during the 2009 April 6 Mw 6.3 earthquake. The city is settled on a top flat hill, with a shear-wave velocity profile characterized by a reversal of velocity at a depth of the order of 50–100 m, corresponding to the contact between calcareous breccia and lacustrine deposits. In the southern sector of downtown, a thin unit of superficial red soils causes a further shallow impedance contrast that may have influenced the damage distribution during the 2009 earthquake. In this paper, the main features of ambient seismic vibrations have been studied in the entire city centre by using array measurements. We deployed six 2-D arrays of seismic stations and 1-D array of vertical geophones. The 2-D arrays recorded ambient noise, whereas the 1-D array recorded signals produced by active sources. Surface-wave dispersion curves have been measured by array methods and have been inverted through a neighbourhood algorithm, jointly with the H/V ambient noise spectral ratios related to Rayleigh waves ellipticity. We obtained shear-wave velocity (Vs) profiles representative of the southern and northern sectors of downtown L'Aquila. The theoretical 1-D transfer functions for the estimated Vs profiles have been compared to the available empirical transfer functions computed from aftershock data analysis, revealing a general good agreement. Then, the Vs profiles have been used as input for a deconvolution analysis aimed at deriving the ground motion at bedrock level. The deconvolution has been performed by means of EERA and STRATA codes, two tools commonly employed in the geotechnical engineering community to perform equivalent-linear site response studies. The waveform at the bedrock level has been obtained deconvolving the 2009 main shock recorded at a strong motion station installed in downtown. Finally, this deconvolved waveform has been used as seismic input for evaluating synthetic time-histories in a strong-motion target site located in the middle Aterno river valley. As a target site, we selected the strong-motion station of AQV 5 km away from downtown L'Aquila. For this site, the record of the 2009 L'Aquila main shock is available and its surface stratigraphy is adequately known making possible to propagate the deconvolved bedrock motion back to the surface, and to compare recorded and synthetic waveforms.
    Beschreibung: Published
    Beschreibung: 848–866
    Beschreibung: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Fourier analysis, Earthquake ground motions , Site effects ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 23
    Publikationsdatum: 2020-05-27
    Beschreibung: We propose an innovative approach to mapping CMB topography from seismic P-wave trav- eltime inversions: instead of treating mantle velocity and CMB topography as independent parameters, as has been done so far, we account for their coupling by mantle flow, as formulated by Forte & Peltier. This approach rests on the assumption that P data are sufficiently sensitive to thermal heterogeneity, and that compositional heterogeneity, albeit important in localized regions of the mantle (e.g. within the D′′ region), is not sufficiently strong to govern the pattern of mantle-wide convection and hence the CMB topography. The resulting tomographic maps of CMB topography are physically sound, and they resolve the known discrepancy between images obtained from classic tomography on the basis of core-reflected and core-refracted seismic phases. Since the coefficients of mantle velocity structure are the only free parameters of the inversion, this joint tomography–geodynamics approach reduces the number of param- eters; nevertheless the corresponding mantle models fit the seismic data as well as the purely seismic ones.
    Beschreibung: Published
    Beschreibung: 730-746
    Beschreibung: 1T. Geodinamica e interno della Terra
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Seismic tomography ; 04. Solid Earth::04.01. Earth Interior::04.01.03. Mantle and Core dynamics
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 24
    Publikationsdatum: 2017-04-04
    Beschreibung: We present the results of palaeomagnetic analysis on Late Bronge Age pottery from Santorini carried out in order to estimate the thermal effect of the Minoan eruption on the pre-Minoan habitation level. A total of 170 specimens from 108 ceramic fragments have been studied. The ceramics were collected from the surface of the pre-Minoan palaeosol at six different sites, including also samples from the Akrotiri archaeological site. The deposition temperatures of the first pyroclastic products have been estimated by the maximum overlap of the re-heating temperature intervals given by the individual fragments at site level. A new statistical elaboration of the temperature data has also been proposed, calculating at 95 per cent of probability the re-heating temperatures at each site. The obtained results show that the precursor tephra layer and the first pumice fall of the eruption were hot enough to re-heat the underlying ceramics at temperatures 160–230 ◦C in the non-inhabited sites while the temperatures recorded inside the Akrotiri village are slightly lower, varying from 130 to 200 ◦C. The decrease of the temperatures registered in the human settlements suggests that there was some interaction between the buildings and the pumice fallout deposits while probably the buildings debris layer caused by the preceding and syn-eruption earthquakes has also contributed to the decrease of the recorded re-heating temperatures.
    Beschreibung: Published
    Beschreibung: 33-47
    Beschreibung: 1A. Geomagnetismo e Paleomagnetismo
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Archaeomagnetism ; Rock and mineral magnetism ; Volcaniclastic deposits ; 04. Solid Earth::04.05. Geomagnetism::04.05.06. Paleomagnetism ; 04. Solid Earth::04.05. Geomagnetism::04.05.07. Rock magnetism ; 04. Solid Earth::04.08. Volcanology::04.08.08. Volcanic risk ; 05. General::05.02. Data dissemination::05.02.03. Volcanic eruptions
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 25
    Publikationsdatum: 2017-04-04
    Beschreibung: Probabilistic tsunami hazard analysis (PTHA) relies on computationally demanding numerical simulations of tsunami generation, propagation, and non-linear inundation on high-resolution topo-bathymetric models. Here we focus on tsunamis generated by co-seismic sea floor dis- placement, that is, on Seismic PTHA (SPTHA). A very large number of tsunami simulations are typically needed to incorporate in SPTHA the full expected variability of seismic sources (the aleatory uncertainty). We propose an approach for reducing their number. To this end, we (i) introduce a simplified event tree to achieve an effective and consistent exploration of the seismic source parameter space; (ii) use the computationally inexpensive linear approximation for tsunami propagation to construct a preliminary SPTHA that calculates the probability of maximum offshore tsunami wave height (H Max) at a given target site; (iii) apply a two-stage filtering procedure to these ‘linear’ SPTHA results, for selecting a reduced set of sources and (iv) calculate ‘non-linear’ probabilistic inundation maps at the target site, using only the selected sources. We find that the selection of the important sources needed for approximating probabilistic inundation maps can be obtained based on the offshore HMax values only. The filtering procedure is semi-automatic and can be easily repeated for any target sites. We describe and test the performances of our approach with a case study in the Mediterranean that considers potential subduction earthquakes on a section of the Hellenic Arc, three target sites on the coast of eastern Sicily and one site on the coast of southern Crete. The comparison between the filtered SPTHA results and those obtained for the full set of sources indicates that our approach allows for a 75–80 per cent reduction of the number of the numerical simulations needed, while preserving the accuracy of probabilistic inundation maps to a reasonable degree.
    Beschreibung: Published
    Beschreibung: 574-588
    Beschreibung: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Tsunami ; Hazard ; Probabilistic ; Subduction ; Mediterranean ; SPTHA ; 04. Solid Earth::04.06. Seismology::04.06.02. Earthquake interactions and probability ; 04. Solid Earth::04.07. Tectonophysics::04.07.04. Plate boundaries, motion, and tectonics ; 04. Solid Earth::04.07. Tectonophysics::04.07.06. Subduction related processes
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 26
    Publikationsdatum: 2017-04-04
    Beschreibung: Temporal variations in the elastic behaviour of the Earth’s crust can be monitored through the analysis of the Earth’s seismic response and its evolution with time. This kind of analysis is particularly interesting when combined with the reconstruction of seismic Green’s functions from the cross-correlation of ambient seismic noise, which circumvents the limitations imposed by a dependence on the occurrence of seismic events. In fact, because seismic noise is recorded continuously and does not depend on earthquake sources, these cross-correlation functions can be considered analogously to records from continuously repeating doublet sources placed at each station, and can be used to extract observations of variations in seismic velocities. These variations, however, are typically very small: of the order of 0.1 per cent. Such accuracy can be only achieved through the analysis of the full reconstructed waveforms, including later scattered arrivals. We focus on the method known as Moving-Window Cross-Spectral Analysis that has the advantage of operating in the frequency domain, where the bandwidth of coherent signal in the correlation function can be clearly defined. We investigate the sensitivity of this method by applying it to microseismic noise cross-correlations which have been perturbed by small synthetic velocity variations and which have been randomly contaminated. We propose threshold signal-to-noise ratios above which these perturbations can be reliably observed. Such values are a proxy for cross-correlation convergence, and so can be used as a guideline when determining the length of microseismic noise records that are required before they can be used for monitoring with the moving-window cross-spectral technique.
    Beschreibung: Published
    Beschreibung: 867-882
    Beschreibung: 4T. Fisica dei terremoti e scenari cosismici
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Interferometry; Volcano monitoring ; 04. Solid Earth::04.02. Exploration geophysics::04.02.06. Seismic methods
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 27
    Publikationsdatum: 2017-04-04
    Beschreibung: In this study we have investigated the forward directivity associated with the initial up-dip rupture propagation during the April 6th 2009 (MW 6.1) L’Aquila normal-faulting earthquake. The objective is the understanding of how the peculiar initial behavior of rupture history during the main shock has affected the near-source recorded ground motions in the L’Aquila town and surrounding areas. We have modeled the observed ground velocities at the closest near-source recording sites by computing synthetic seismograms using a discrete wavenumbers and finite difference approach in the low frequency bandwidth (0.02-0.4 Hz) to avoid site effects contaminations. We use both the rupture model retrieved by inverting ground motion waveforms and continuous high sampling-rate GPS time series as well as uniform-slip constant-rupture speed models. Our results demonstrate that the initial up-dip rupture propagation, characterizing the first three seconds of the rupture history during the L’Aquila main shock and releasing only ∼25% of total seismic moment, controls the observed ground motions in the near-source. This initial stage of the rupture is characterized by the generation of clear ground velocity pulses, which we interpret as a forward directivity effect. Our modeling results confirm a heterogeneous distribution of rupture velocity during the initial up-dip rupture propagation, since uniform rupture speed models overestimate up-dip directivity effects in the footwall of the causative fault. The up-dip directivity observed in the near field during the 2009 L’Aquila main shock is that predicted for a normal faulting earthquake by Somerville’s directivity model, but it differs from that inferred from far-field observations that conversely provide evidence of along-strike directivity. This calls for a careful analysis as well as for the realistic inclusion of rupture directivity to predict ground motions in the near source.
    Beschreibung: Published
    Beschreibung: 1618-1631
    Beschreibung: 4T. Fisica dei terremoti e scenari cosismici
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): earthquake ground motion, earthquake source observations, computational seismology ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 28
    facet.materialart.
    Unbekannt
    Wiley-Blackwell
    In:  Magnitude conversion problem using general orthogonal regression’ by H. R. Wason, Ranjit Das and M. L. Sharma, (Geophys. J. Int., 190, 1091–1096)
    Publikationsdatum: 2017-04-04
    Beschreibung: The argument proposed by Wason et al. that the conversion of magnitudes from a scale (e.g. Ms or mb) to another (e.g. Mw), using the coefficients computed by the general orthogonal regression method (Fuller) is biased if the observed values of the predictor (independent) variable are used in the equation as well as the methodology they suggest to estimate the supposedly true values of the predictor variable are wrong for a number of theoretical and empirical reasons. Hence, we advise against the use of such methodology for magnitude conversions.
    Beschreibung: Published
    Beschreibung: 626-627
    Beschreibung: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Earthquake source observations ; Statistical seismology ; Computational seismology ; 05. General::05.01. Computational geophysics::05.01.04. Statistical analysis
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 29
    facet.materialart.
    Unbekannt
    Wiley-Blackwell
    Publikationsdatum: 2017-04-04
    Beschreibung: Fluids—essentially meteoric water—are present everywhere in the Earth’s crust, occasionally also with pressures higher than hydrostatic due to the tectonic strain imposed on impermeable undrained layers, to the impoundment of artificial lakes or to the forced injections required by oil and gas exploration and production. Experimental evidence suggests that such fluids flow along preferred paths of high diffusivity, provided by rock joints and faults. Studying the coupled poroelastic problem, we find that such flow is ruled by a nonlinear partial differential equation amenable to a Barenblatt-type solution, implying that it takes place in formof solitary pressure waves propagating at a velocity which decreases with time as v ∝t [1/(n − 1) − 1] with n 7. According to Tresca-Von Mises criterion, these waves appear to play a major role in earthquake triggering, being also capable to account for aftershock delay without any further assumption. The measure of stress and fluid pressure inside active faults may therefore provide direct information about fault potential instability.
    Beschreibung: Published
    Beschreibung: 1281–1285
    Beschreibung: 6T. Sismicità indotta e caratterizzazione sismica dei sistemi naturali
    Beschreibung: N/A or not JCR
    Beschreibung: restricted
    Schlagwort(e): forecasting and prediction ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 30
    Publikationsdatum: 2017-04-04
    Beschreibung: Our improved capability to adapt to future changes in discharge is linked to our capability to predict the magnitude or at least the direction of these changes. For the agricultural U.S. Midwest, too much or too little water has severe socio-economic impacts. Here we focus on the Raccoon River at Van Meter, Iowa, and use a statistical approach to examine projected changes in discharge. We build on statistical models using rainfall and harvested corn and soybean acreage to explain the observed discharge variability. We then use projections of these two predictors to examine the projected discharge response. Results are based on seven global climate models part of the Coupled Model Intercomparison Project Phase 5 and two representative concentration pathways (RCPs 4.5 and 8.5). There is not a strong signal of change in the discharge projections under the RCP 4.5. However the results for the RCP 8.5 point to a stronger changing signal related to larger projected increases in rainfall, resulting in increasing trends in particular in the upper part of the discharge distribution (i.e., 60th percentile and above). Examination of two hypothetical agricultural scenarios indicates that these increasing trends could be alleviated by decreasing the extent of the agricultural production. We also discuss how the methodology presented in this study represents a viable approach to move forward with the concept of return period for engineering design and management in a non-stationary world.
    Beschreibung: Published
    Beschreibung: 1361–1371
    Beschreibung: 4A. Clima e Oceani
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): river discharge ; rainfall ; statistical model ; 01. Atmosphere::01.01. Atmosphere::01.01.02. Climate
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 31
    Publikationsdatum: 2017-04-04
    Beschreibung: We present a strategy to thoroughly investigate the effects of prominent topography on the surface tilt due to a spherical pressure source. We use Etna's topography as a case of study and, for different source positions, we compare the tilt fields calculated through (i) a 3-D boundary element method and (ii) analytical half-space solutions. We systematically determine (i) the source positions leading to the strongest tilt misfits when numerical and analytical results are compared and (ii) the surface areas where the strongest distortions in the tilt field are most likely to be observed. We also demonstrate that, under critical circumstances, in terms of respective positions of pressure source and observation points, results of inversion procedures aimed at retrieving the source parameters can be misleading, if tilt data are analysed using models that do not account for topography.
    Beschreibung: Published
    Beschreibung: 1471–1481
    Beschreibung: 2V. Dinamiche di unrest e scenari pre-eruttivi
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Numerical approximations and analysis ; Transient deformation ; Volcano monitoring ; 04. Solid Earth::04.03. Geodesy::04.03.01. Crustal deformations
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 32
    Publikationsdatum: 2017-12-11
    Beschreibung: In this study we applied a multidisciplinary approach, coupling geophysical and geochemical measurements, to unveil the provenance of 170 obsidian flakes, collected on the volcanic island of Ustica (Sicily). On this island there are some prehistoric settlements dated from the Neolithic to the Middle Bronze Age. Despite not having geological outcrops of obsidian rocks, the countryside of Ustica is rich in fragments of this volcanic glass, imported from other source areas. The study of obsidian findings was carried out first through visual observations and density measurements. At least two different obsidian families have been distinguished, probably imported from Lipari and Pantelleria islands. Analysing the magnetic properties of the samples, these two main sources were confirmed, but the possibility of other provenances was inferred. Finally, we characterized the geochemical signature of the Ustica obsidians by performing microchemical analyses through electron microprobe (EMPA) and laser ablation (LA–ICP–MS). The results were compared with literature data, confirming the presence of the Lipari and Pantelleria sources (Sicily) and indicating for the first time in this part of Italy a third provenance from Palmarola island (Latium). Our results shed new light on the commercial exchanges in the peri-Tyrrhenian area during the prehistoric age.
    Beschreibung: Published
    Beschreibung: 435–454
    Beschreibung: 1SR. TERREMOTI - Servizi e ricerca per la Società
    Beschreibung: 2SR. VULCANI - Servizi e ricerca per la Società
    Beschreibung: 3SR. AMBIENTE - Servizi e ricerca per la Società
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): obsdian provenance ; LA-ICPMS ; 05. General::05.04. Instrumentation and techniques of general interest::05.04.99. General or miscellaneous
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 33
    Publikationsdatum: 2018-03-20
    Beschreibung: Our study area is a ~50 km long section of the central-southern Apennines tectonic belt that includes the Pergola-Melandro basin and the Agri valley. This region is located between the areas interested by the 1980 Ms=6.9 Irpinia and the 1857 M=7.0 Val d’Agri earthquakes and is characterized by rare historical events and very low and sparse background seismicity. In this study we provide new seismological and geophysical information to identify the characteristics of the seismotectonics in the area, as the prevailing faulting mechanism and the fit of local to regional stress field. These data concern focal mechanisms from waveform modeling and P-wave polarities, analyses of borehole breakouts and detailed investigation of two seismic sequences. All the data cover a significantly broad range of magnitudes and depths and suggest that no important local variation in stress orientation seems to affect this area, which shows a NE-SW direction of extension consistent with that regionally observed in Southern Italy. Such local homogeneity in the stress field pattern is peculiar of the study area; the variations of orientation and/or type of stress observed in the northern Apennines or only less than 100 km toward the northwest within the same tectonic belt are absent here. Furthermore, there is a suggestion for a northeastward sense of dip of the seismogenic faults in the region, an interesting constraint to the characterization of seismic sources
    Beschreibung: Published
    Beschreibung: 575-583
    Beschreibung: 2T. Sorgente Sismica
    Beschreibung: JCR Journal
    Schlagwort(e): faulting ; seismicity ; 04. Solid Earth::04.07. Tectonophysics::04.07.05. Stress
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 34
    Publikationsdatum: 2020-02-24
    Beschreibung: Metal-catalysed CO2 hydrogenation is considered a source of methane in serpentinized (hydrated) igneous rocks and a fundamental abiotic process germane to the origin of life. Iron, nickel, chromium and cobalt are the catalysts typically employed in hydrothermal simulation experiments to obtain methane at temperatures 〉200°C. However, land-based present-day serpentinization and abiotic gas apparently develop below 100°C, down to approximately 40–50°C. Here, we document considerable methane production in thirteen CO2 hydrogenation experiments performed in a closed dry system, from 20 to 90°C and atmospheric pressure, over 0.9–122 days, using concentrations of non-pretreated ruthenium equivalent to those occurring in chromitites in ophiolites or igneous complexes (from 0.4 to 76 mg of Ru, equivalent to the amount occurring approximately in 0.4–760 kg of chromitite). Methane production increased with time and temperature, reaching approximately 87 mg CH4 per gram of Ru after 30 days (2.9 mgCH4/gru/day) at 90°C. At room temperature, CH4 production rate was approximately three orders of magnitude lower (0.003 mgCH4/gru/day). We report the first stable carbon and hydrogen isotope ratios of abiotic CH4 generated below 100°C. Using initial d13CCO2 of -40&, we obtained room temperature d13CCH4 values as 13C depleted as 142&. With time and temperature, the C-isotope separation between CO2 and CH4 decreased significantly and the final d13CCH4 values approached that of initial d13CCO2. The presence of minor amounts of C2-C6 hydrocarbons is consistent with observations in natural settings. Comparative experiments at the same temperatures with iron and nichel catalysts did not generate CH4. Ru-enriched chromitites could potentially generate methane at low temperatures on Earth and on other planets.
    Beschreibung: Published
    Beschreibung: 438–452
    Beschreibung: 7A. Geofisica di esplorazione
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): abiotic methane, Sabatier reaction ; 04. Solid Earth::04.04. Geology::04.04.12. Fluid Geochemistry
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 35
    Publikationsdatum: 2020-02-05
    Beschreibung: In this paper, we present a method for handling uncertainties in the determination of the source parameters of earthquakes from spectral data. We propose a robust framework for estimating earthquake source parameters and relative uncertainties, which are propagated down to the estimation of basic seismic parameters of interest such as the seismic moment, the moment magnitude, the source size and the static stress drop. In practice, we put together a Bayesian approach for model parameter estimation and a weighted statistical mixing of multiple solutions obtained from a network of instruments, providing a useful framework for extracting meaningful data from intrinsically uncertain data sets. The Bayesian approach used to estimate the source spectra parameters is a simple but powerful mechanism for non-linear model fitting, providing also the opportunity to naturally propagate uncertainties and to assess the quality and uniqueness of the solution. Another important added value of such an approach is the possibility of integrating information from the expertise of seismologists. Such data can be encoded in a prior state of information that is then updated with the information provided by seismological data. The performance of the proposed approach is demonstrated analysing data from the 1909 April 23 earthquake occurred near Benavente (Portugal).
    Beschreibung: Published
    Beschreibung: 691-701
    Beschreibung: 2T. Tettonica attiva
    Beschreibung: JCR Journal
    Beschreibung: open
    Schlagwort(e): Fourier analysis ; Probability distributions ; Earthquake source observations ; Seismicity and tectonics ; 04. Solid Earth::04.02. Exploration geophysics::04.02.06. Seismic methods
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 36
    Publikationsdatum: 2017-04-04
    Beschreibung: The elevation of the Capo Vaticano coastal terraces (Tyrrhenian coast, central Calabria) is the result of a combination of regional uplift and repeated coseismic displacement. We subtract the regional uplift from the total uplift (maximum average uplift rates: 0.81–0.97 mm a)1 since c. 0.7 Ma) and obtain the residual fault-related displacement. Then, we model the residual displacement to provide constraints on the location and geometry of the seismogenic source of the 1905 M7 earthquake, the strongest – and still poorly understood – earthquake of the instrumental era in this area. We try four different potential sources for the dislocation modelling and find that (1) three sources are not compatible with the displacement observed along the terraces and (2) the only source consistent with the local deformation is the 100 - striking Coccorino Fault. We calculate average long-term vertical slip rates of 0.2–0.3 mm a)1 on the Coccorino Fault and estimate an average recurrence time of one millennium for a 1905-type earthquake.
    Beschreibung: Published
    Beschreibung: 378-389
    Beschreibung: 2T. Tettonica attiva
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): 1905 earthquake ; marine terraces ; coseismic displacement ; 04. Solid Earth::04.04. Geology::04.04.01. Earthquake geology and paleoseismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 37
    Publikationsdatum: 2017-04-04
    Beschreibung: Real-time seismology has made significant improvements in recent years, with source parameters now available within a few tens of minutes after an earthquake. It is likely that this time will be further reduced, in the near future, by means of increased efficiency in real-time transmission,increasingdatacoverageandimprovementofthemethodologies.Inthiscontext, together with the development of new ground motion predictive equations (GMPEs) that are abletoaccountforsourcecomplexity,thegenerationofstronggroundmotionshakingmapsin quasi-real time has become ever more feasible after the occurrence of a damaging earthquake. However, GMPEs may not reproduce reliably the ground motion in the near-source region where the finite fault parameters have a strong influence on the shaking. Inthispaperwetestwhetheraccountingforsource-relatedeffectsiseffectiveinbettercharacterizingthegroundmotion.WeintroduceamodificationoftheGMPEswithintheShakeMap softwarepackage,andsubsequentlytesttheaccuracyofthenewlygeneratedshakemapsinpredictingthegroundmotion.ThetestisconductedbycontrollingtheperformanceofShakeMap as we decrease the amount of the available information. We then update ShakeMap with the GMPE modified with a corrective factor accounting for source effects, in order to better constrain these effects that likely influence the level of (near-source) ground shaking. Weinvestigatetwowell-recordedearthquakesfromJapan(the2000Tottori, Mw 6.6,andthe 2008 Iwate-Miyagi, Mw7.0, events) where the instrumental coverage is as dense as needed to ensure an objective appraisal of the results. The results demonstrate that the corrected GMPE can capture only some aspects of the ground shaking in the near-source area, neglecting other multidimensional effects, such as propagation effects and local site amplification.
    Beschreibung: Italian Presidenza del Consiglio dei Ministri, Dipartimento della Protezione Civile(DPC)under the contract 2007–2009 DPC-INGVS3project
    Beschreibung: Published
    Beschreibung: 1836-1848
    Beschreibung: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Beschreibung: 4T. Fisica dei terremoti e scenari cosismici
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Earthquake ground motions ; Earthquake source observation ; Computational seismology ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion ; 04. Solid Earth::04.06. Seismology::04.06.10. Instruments and techniques ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 38
    Publikationsdatum: 2017-04-04
    Beschreibung: A recent study of the Matuyama–Brunhes (M-B) geomagnetic field reversal recorded in exposed lacustrine sediments from the Sulmona Basin (Italy) provided a continuous, highresolution record indicating that the reversal of the field direction at the terminus of the M-B boundary (MBB) occurred in less than a century, about 786 ka ago. In the sediment, thin (4–6 cm) remagnetized horizons were recognized above two distinct tephra layers—SUL2- 19 and SUL2-20—that occur ∼25 and ∼35 cm below the MBB, respectively. Also, a faint, millimetre-thick tephra (SUL2-18) occurs 2–3 cm above the MBB.With the aim of improving the temporal resolution of the previous Sulmona MBB record and understanding the possible influence of cryptotephra on the M-B record in the Sulmona Basin,we performed more detailed sampling and analyses of overlapping standard and smaller samples from a 50 cm-long block that spans the MBB. The new data indicate that (i) the MBB is even sharper than previously reported and occurs ∼2.5 cm below tephra SUL2-18, in agreement with the previous study; (ii) the MBB coincides with the rise of an intensity peak of the natural remanent magnetization (NRM) intensity, which extends across SUL2-18; (iii) except for a 2-cm-thick interval just above tephra SUL2-18, the rock magnetic parameters (k, ARM, Mr, Ms, Bc, Bcr) indicate exactly the same magnetic mineralogy throughout the sampled sequence. We conclude that either SUL2-18 resulted in the remagnetization of an interval of about 6 cm (i.e. during the NRM intensity peak spanning ∼260 ± 110 yr, according to the estimated local sedimentation rate), and thus the detailed MBB record is lost because it is overprinted, or the MBB is well recorded, occurred abruptly about 2.5 cm below SUL2-18 and lasted less than 13 ± 6 yr. Both hypotheses challenge our understanding of the geomagnetic field behaviour during a polarity transition and/or of the NRM acquisition process in the Sulmona lacustrine sediment.
    Beschreibung: Published
    Beschreibung: 798-812
    Beschreibung: 1A. Geomagnetismo e Paleomagnetismo
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Palaeomagnetic secular variation; Rapid time variations; Reversals: process, time scale, magnetostratigraphy; Rock and mineral magnetism ; 04. Solid Earth::04.05. Geomagnetism::04.05.06. Paleomagnetism
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 39
    Publikationsdatum: 2021-11-25
    Beschreibung: On 2012 May 20 and 29, two damaging earthquakes with magnitudes Mw 6.1 and 5.9, respectively, struck the Emilia-Romagna region in the sedimentary Po Plain, Northern Italy, causing 26 fatalities, significant damage to historical buildings and substantial impact to the economy of the region. The earthquake sequence included four more aftershocks with Mw ? 5.0, all at shallow depths (about 7–9 km), with similar WNW–ESE striking reverse mechanism. The timeline of the sequence suggests significant static stress interaction between the largest events. We perform here a detailed source inversion, first adopting a point source approximation and considering pure double couple and full moment tensor source models. We compare different extended source inversion approaches for the two largest events, and find that the rupture occurred in both cases along a subhorizontal plane, dipping towards SSW. Directivity is well detected for the May 20 main shock, indicating that the rupture propagated unilaterally towards SE. Based on the focal mechanism solution, we further estimate the co-seismic static stress change induced by the May 20 event. By using the rate-and-state model and a Poissonian earthquake occurrence, we infer that the second largest event of May 29 was induced with a probability in the range 0.2–0.4. This suggests that the segment of fault was already prone to rupture. Finally, we estimate peak ground accelerations for the two main events as occurred separately or simultaneously. For the scenario involving hypothetical rupture areas of both main events, we estimate Mw = 6.3 and an increase of ground acceleration by 50 per cent. The approach we propose may help to quantify rapidly which regions are invested by a significant increase of the hazard, bearing the potential for large aftershocks or even a second main shock.
    Beschreibung: Published
    Beschreibung: 1658-1672
    Beschreibung: 4T. Fisica dei terremoti e scenari cosismici
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Earthquake dynamics ; Earthquake source observations ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 40
    Publikationsdatum: 2022-05-24
    Beschreibung: Close relationships between deformation and volcanism are well documented in relatively late evolutionary stages of con- tinental rifting, whereas these are poorly constrained in less mature rifting stages. To investigate the control of inherited structures on faulting and volcanism, we present a statistical analysis of volcanic features, faults and pre-rift fabric in the Tanzania Divergence, where volcanic features occur exten- sively in in-rift and off-rift areas. Our results show that in mature rift sectors (Natron), magma uprising is mostly con- trolled by fractures/faults responding to the far-field stress, whereas the distribution of volcanism during initial rifting (Eyasi) is controlled by inherited structures oblique to the regional extension direction. Off-rift sectors show a marked control of pre-rift structures on magma emplacement, which may not respond to the regional stress field. Thus, the use of off-rift magmatic features as stress indicators should take into account the role of pre-existing structures.
    Beschreibung: Published
    Beschreibung: 461-468
    Beschreibung: 1T. Geodinamica e interno della Terra
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): East Africa Rift System, Tanzania-Kenia, structures and volcanism ; 04. Solid Earth::04.07. Tectonophysics::04.07.02. Geodynamics
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 41
    Publikationsdatum: 2022-05-24
    Beschreibung: Pico, the youngest island of the Azores Archipelago (Portugal), is characterized by a central volcano and a 30-km-long fissure zone. Its eruption rate is the highest of the Azores islands, with more than 35 eruptions in the last 2000 years. Here, we estimate the lava-flow hazard for Pico Island by combining the vent opening probability derived from the spatial distribution of eruptive fissures, the classes of expected eruptions inferred from the physical and chemical characteristics of historical eruptions, and the lava-flow paths simulated by the MAGFLOW model. The most likely area to host new eruptions is along a WNW–ESE trend centred on the central volcano, with the highest hazard affecting the two main residential zones of Lajes do Pico and Madalena. Our analysis is the first attempt to assess the lava-flow hazard for Pico Island, and may have important implications for decision-making in territorial management and future land-use planning.
    Beschreibung: Published
    Beschreibung: 156-161
    Beschreibung: 3V. Dinamiche e scenari eruttivi
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): lava flow hazard ; 05. General::05.02. Data dissemination::05.02.03. Volcanic eruptions
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 42
    Publikationsdatum: 2022-06-10
    Beschreibung: Patagonia Argentina is a key area for the study of sea level changes in the southern hemisphere, but the availability of reliable sea level markers in this area is still problematic. In fact the storm deposits (beach ridge) commonly used here to reconstruct past sea level oscillations introduce a wide error. Along the Puerto Deseado coast (Santa Cruz), morphometric analyses of 11 features were carried out using traditional measurement tools and a digital software-based method (tested on one selected feature) with the aim to investigate the possibility of their use as sea level markers. By undertaking accurate topographic profiles we identified the relationship between notches and current sea level. In detail, we identified two clusters of notch retreat point elevations, with a very low internal variability. The lower was located a little below the mean high tide level (mHT) and the upper located at least 0.5m above the maximum high tide level (MHT). Field observations of tidal levels and the position of notches suggest that the lower notches are active and the upper are inactive. This study on the abrasive notches attests their quality as sea level markers and opens up the use of fossil abrasive notches as palaeo sea level markers because the error linked to these features is substantially smaller than that introduced by beach ridges commonly used in the study area
    Beschreibung: Published
    Beschreibung: 1550 – 1558
    Beschreibung: 4A. Clima e Oceani
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): notch; rocky coast; sea level marker; Patagonia; Argentina ; 01. Atmosphere::01.01. Atmosphere::01.01.02. Climate
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 43
    Publikationsdatum: 2022-06-14
    Beschreibung: Rayleigh wave group velocity dispersion measurements from local and regional earthquakes are used to interpret the lithospheric structure in the Gulf of California region. We compute group velocity maps for Rayleigh waves from 10 to 150 s using earthquakes recorded by broadband stations of the Network of Autonomously Recording Seismographs in Baja California and Mexico mainland, UNM in Mexico, BOR, DPP and GOR in southern California and TUC in Arizona. The study area is gridded in 120 longitude cells by 180 latitude cells, with an equal spacing of 10 × 10 km. Assuming that each gridpoint is laterally homogeneous, for each period the tomographic maps are inverted to produce a 3-D lithospheric shear wave velocity model for the region. Near the Gulf of California rift axis, we found three prominent low shear wave velocity regions, which are associated with mantle upwelling near the Cerro Prieto volcanic field, the Ballenas Transform Fault and the East Pacific Rise. Upwelling of the mantle at lithospheric and asthenospheric depths characterizes most of the Gulf. This more detailed finding is new when compared to previous surface wave studies in the region. A low-velocity zone in northcentral Baja at ∼28oN which extends east–south–eastwards is interpreted as an asthenospheric window. In addition, we also identify a well-defined high-velocity zone in the upper mantle beneath central-western Baja California, which correlates with the previously interpreted location of the stalled Guadalupe and Magdalena microplates. We interpret locations of the fossil slab and slab window in light of the distribution of unique post-subduction volcanic rocks in the Gulf of California and Baja California.We also observe a high-velocity anomaly at 50-km depth extending down to ∼130 km near the southwestern Baja coastline and beneath Baja, which may represent another remnant of the Farallon slab.
    Beschreibung: Published
    Beschreibung: 1861-1877
    Beschreibung: 1T. Geodinamica e interno della Terra
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): surface waves ; seismic tomography ; dynamics of lithosphere and mantle ; crustal structure ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 44
    Publikationsdatum: 2022-05-24
    Beschreibung: Earthquake source inversions based on space-borne Synthetic Aperture Radar interferometry (InSAR) are used extensively. Typically, however, only the line-of-sight (LoS) surface displacement component is measured, which is mainly sensitive to the vertical and E–W deformations, although well-established methods also exist to estimate the flight-path component, which is highly sensitive to the N–S displacement. With high-resolution sensors, these techniques are particularly appealing, because accuracies in the order of 3 cm can be achieved, while retaining spatial resolutions between 45 m and a few km, depending on the required level of filtering. We discuss the application to COSMO-SkyMed SAR imagery of the Spectral Diversity or Multi Aperture Interferometry technique, presenting the first SAR flight-path displacement field associated with the Mw 6.3, 2009 L’Aquila event (central Apennines). Finally, we observe and characterize a previously unknown misregistration pattern.
    Beschreibung: Published
    Beschreibung: 28-35
    Beschreibung: 2T. Tettonica attiva
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Crustal Deformation ; Multi Aperture Interferometry MAI ; InSAR ; L'Aquila Earthquake ; Satellite geodesy ; 04. Solid Earth::04.03. Geodesy::04.03.01. Crustal deformations
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 45
    Publikationsdatum: 2022-05-24
    Beschreibung: We present an application of the novel SISTEM approach, to obtain the dense 3D ground deformation pattern produced by the April 6, 2009, Mw 6.3 L’Aquila earthquake. This event, characterized by a SW-dipping normal fault with thousands of foreshocks and aftershocks located in the depth range 5–15 km, is the most destructive to have struck the Abruzzo region since the major 1703 seismic sequence. The surface deformation, revealed by the SISTEM through the integration of GPS with interferometric measurements from the ENVISAT and ALOS satellites, shows a deformed area extending towards SE along the Aterno valley, in agreement with seismological and other geodetic observations. We inverted the SISTEM results using an optimization algorithm based on the genetic algorithm, providing an accurate spatial characterization of ground deformation. Our results improve previous kinematic solutions for the Paganica fault and allow identification of additional faults that have contributed to the observed complex ground deformation pattern.
    Beschreibung: Published
    Beschreibung: 79-85
    Beschreibung: 4T. Fisica dei terremoti e scenari cosismici
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): L'Aquila earthquake, SISTEM, GPS ; 04. Solid Earth::04.03. Geodesy::04.03.01. Crustal deformations
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 46
    Publikationsdatum: 2022-05-24
    Beschreibung: This study investigates in detail the deformation events that have affected the sedimentary successions forming the substrate of Mt. Etna volcano (Italy). Based on the geometric reconstruction of a buried sedimentary marker, we have been able to identify and quantify the effects of three different mechanisms of deformation that have affected the area in the last 600 ka. Numerical results from Finite Element Method (FEM) applied to model viscoelastic deformation suggest the occurrence of a crustal doming process originating at the mantle-crust transition (~16 km). We propose that the source of deformation is related to the diapiric uprise of hydrothermal material originating in altered ocean-like crust and its emplacement at a shallower level in the crust. This process has great relevance in the volcanic system and should be considered for the full assessment of its origin and evolution.
    Beschreibung: Published
    Beschreibung: 338 – 345
    Beschreibung: 4V. Vulcani e ambiente
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): volcanic doming ; viscoelastic modeling ; 04. Solid Earth::04.08. Volcanology::04.08.99. General or miscellaneous
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 47
    Publikationsdatum: 2022-05-24
    Beschreibung: Continuous GPS (CGPS) data, collected at Mt. Etna between April 2012 and October 2013, clearly define inflation/deflation processes typically observed before/after an eruption onset. During the inflationary process from May to October 2013, a particular deformation pattern localised in the upper North Eastern sector of the volcano suggests that a magma intrusion had occurred a few km away from the axis of the summit craters, beneath the NE Rift system. This is the first time that this pattern has been recorded by CGPS data at Mt. Etna. We believe that this inflation process might have taken place periodically at Mt. Etna and might be associated with the intrusion of batches of magma that are separate from the main feeding system. We provide a model to explain this unusual behaviour and the eruptive regime of this rift zone, which is characterised by long periods of quiescence followed by often dangerous eruptions in which vents can open at low elevation and thus threaten the villages in this sector of the volcano.
    Beschreibung: Published
    Beschreibung: 356-363
    Beschreibung: 2V. Dinamiche di unrest e scenari pre-eruttivi
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Shallow intrusion beneath NE Rift system ; Mt. Etna volcano ; CGPS data ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 48
    Publikationsdatum: 2024-04-05
    Beschreibung: Secondary microseisms recorded by seismic stations are generated in the ocean by the interaction of ocean gravity waves.We present here the theory for modelling secondary microseismic noise by normal mode summation.We show that the noise sources can be modelled by vertical forces and how to derive them from a realistic ocean wave model. We then show how to compute bathymetry excitation effect in a realistic earth model by using normal modes and a comparison with Longuet–Higgins approach. The strongest excitation areas in the oceans depends on the bathymetry and period and are different for each seismic mode. Seismic noise is then modelled by normal mode summation considering varying bathymetry. We derive an attenuation model that enables to fit well the vertical component spectra whatever the station location. We show that the fundamental mode of Rayleigh waves is the dominant signal in seismic noise. There is a discrepancy between real and synthetic spectra on the horizontal components that enables to estimate the amount of Love waves for which a different source mechanism is needed. Finally, we investigate noise generated in all the oceans around Africa and show that most of noise recorded in Algeria (TAM station) is generated in the Northern Atlantic and that there is a seasonal variability of the contribution of each ocean and sea.
    Beschreibung: Published
    Beschreibung: 1732-1745
    Beschreibung: 1T. Geodinamica e interno della Terra
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Surface waves and free oscillations ; Seismic attenuation ; Theoretical seismology ; Wave propagation ; 04. Solid Earth::04.04. Geology::04.04.99. General or miscellaneous
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 49
    Publikationsdatum: 2024-04-05
    Beschreibung: Secondary microseismic noise is generated by non-linear interactions between ocean waves at the ocean surface. We present here the theory for computing the site effect of the ocean layer upon body waves generated by noise sources distributed along the ocean surface. By defining the wavefield as the superposition of plane waves, we show that the ocean site effect can be described as the constructive interference of multiply reflected P waves in the ocean that are then converted to either P or SV waves at the ocean–crust interface. We observe that the site effect varies strongly with period and ocean depth, although in a different way for body waves than for Rayleigh waves. We also show that the ocean site effect is stronger for P waves than for S waves. We validate our computation by comparing the theoretical noise body wave sources with the sources inferred from beamforming analysis of the three seismogram components recorded by the Southern California Seismic Network. We use rotated traces for the beamforming analysis, and we show that we clearly detect P waves generated by ocean gravity wave interactions along the track of typhoon Ioke (2006 September). We do not detect the corresponding SV waves, and we demonstrate that this is because their amplitude is too weak.
    Beschreibung: Published
    Beschreibung: 1096-1106
    Beschreibung: 1T. Geodinamica e interno della Terra
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Body waves ; Site effects ; Theoretical Seismology ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 50
    Publikationsdatum: 2024-05-09
    Beschreibung: The Sulmona plain (central Italy) is an intramontane basin of the Abruzzi Apennines that is known in the literature for its high seismic hazard. We use extensive measurements of ambient noise to map the fundamental frequency and to detect the presence of geological heterogeneities in the basin. We perform noise measurements along two basin-scale orthogonal transects, in conjunction with 2-D array experiments in specific key areas. The key areas are located in different positions with respect to the basin margins: one at the eastern boundary (fault-controlled basin margin) and one in the deepest part of the basin. We also collect independent data by using active seismic experiments (MASW), down-hole and geological surveys to characterize the near-surface geology of the investigated sites. In detail, the H/V noise spectral ratios and 2-D array techniques indicate a fundamental resonance (f0) in the low-frequency range (0.35–0.4 Hz) in the Sulmona Basin. Additionally, our results highlight the important role that is played by the alluvial fans near the edge-sectors of the basin, which are responsible for a velocity inversion in the uppermost layering of the soil profile. The H/V ratios and the dispersion curves of adjacent measurements strongly vary over a few dozens of meters in the alluvial fan area. Furthermore, we perform 1-D numerical simulations that are based on a linear-equivalent approach to estimate the site response in the key areas, using realistic seismic inputs. Finally, we perform a 2-D simulation that is based on the spectral element method to propagate surface waves in a simple model with an uppermost stiff layer, which is responsible for the velocity inversion. The results from the 2-D modelling agree with the experimental curves, showing deamplified H/V curves and typical shapes of dispersion curves of a not normally dispersive site.
    Beschreibung: Published
    Beschreibung: 418-439
    Beschreibung: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Beschreibung: JCR Journal
    Beschreibung: restricted
    Schlagwort(e): Fourier analysis, Earthquake ground motions, Site effects ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 51
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 225-242 
    ISSN: 0886-1544
    Schlagwort(e): spermatozoa ; calcium ion transport ; motility regulation ; cholinergic agonists ; ouabain ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Behavioral responses of mature spermatozoa treated with neurotropic factors suggest that calcium entry and intracellular transport may be regulated by a cholinergic mediated program. To test the validity of this proposed mechanism, the effect of several agents on Ca distribution in the sperm cell was examined cytochemically.Sites of Ca accumulation were visualized in thin sections of bull spermatozoa by the application of a modification of Gomori's histochemical procedure for phosphatases. Intact bull sperm cells incubated at room temperature in a buffered balanced salt solution containing 5 mM/liter of CaCl2 showed small, randomly scattered deposits of the reaction product. Similarly treated sperm cells, plasmolyzed in hypoosmotic KCl, revealed a greatly increased amount of deposit associated with the cell membranes (mitochondrial surfaces and plasmalemma), the axonemal complex components, and satellite fibers adjacent to the outer dense fibers. Preincubation of intact cells in nicotine or eserine considerably enhanced the entry of calcium into the cell and its association with the membranes and other intracellular organelles. Decamethonium, an irreversible depolarizer and blocker of cholinergic receptors, interfered with the uptake and intracellular distribution of the calcium. Ouabain, the digitalis glycoside that decreases progressive motility of bull sperm and inhibits Na-, K-ATPase, appears to block Ca efflux, causing an intense accumulation of electron-opaque particles in the plasma membrane while smaller numbers of particles are distributed sparsely throughout the cell interior.The cytochemical results showing enhanced calcium entry in the presence of cholinergic agents, depressed intracellular calcium in cells treated with cholinergic receptor blocker, and intense accumulation of calcium within the cell membrane in the presence of ouabain are consistent with spermatozoan behavioral responses to these agents. These observations support the concept that neurotropic factors may be involved in regulating transmembrane and intracellular transport of ions in control of sperm cell function.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 52
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 309-315 
    ISSN: 0886-1544
    Schlagwort(e): tropomyosin ; avian muscular dystrophy ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The isotype pattern of tropomyosin was investigated in normal and dystrophic avian pectroal muscle using two-dimensional gel electrophoresis. Previous reports have shown that adult pectoral muscle of chickens contains only the α-subunit of tropomyosin and a breast-type troponin-T (TN-T), whereas pectoral fetal muscle contains both α- and β-tropomyosin and leg-type TN-T. The change from the fetal to the adult forms begins shortly after hatching. It has been previously reported that avian dystrophic pectoral muscle contains both the leg- and breast-type TN-T; we show that in avian dystrophic muscle there is also persistent expression of the β-subunit of tropomyosin.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 53
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982) 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 54
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 287-308 
    ISSN: 0886-1544
    Schlagwort(e): actin-binding protein ; Dictyostelium ; cytoskeleton ; amoeboid movement ; calcium ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: A protein from Dictyostelium discoideum with an apparent subunit molecular weight of 95,000 daltons (95K protein) was previously identified as an actin-binding protein ‘Hellewell and Taylor, 1979’. In this paper, we present a method for purifying the protein, and characterize some important aspects of its structure and function. Purification of the 95K protein is achieved by fractionation with ammonium sulfate followed by chromatography on DEAE-cellulose, gel filtration on 6% agarose, and final purification on hydroxyapatite. The 95K protein is a dimer, composed of apparently identical subunits. It is a rod-shaped molecule, 38 nm in length, with a Stokes radius of 74 Å. In these structural properties, the 95K protein is similar to muscle and nonmuscle α-actinins. The 95K protein and filamin are equally competent, when compared on a weight basis, to enhance the apparent viscosity of actin as determined by falling ball viscometry. The apparent viscosity of mixtures of the 95K protein and actin is dramatically reduced at pH greater than 7.0 or free ‘Ca2+’ greater than 10-7 M. We also examine the mechanism by which calcium regulates the interaction of the 95K protein and actin. A change in free ‘Ca2+’ induces no detectable change in the quaternary structure of the 95K protein. Our experiments indicate that the 95K protein does not dramatically alter the length distribution of actin filaments in the presence of micromolar free ‘Ca2+’. A large fraction of the 95K protein cosediments with actin in the presence of low free ‘Ca2+’ (ca. 3 × 10-8M), but not in the presence of high free ‘Ca2+’ (ca. 4 × 10-6M). We conclude that increased free ‘Ca2+’ inhibits gelation of actin by the 95K protein by reducing the affinity of the 95K protein for actin. We propose that 95K protein is an important component of the cytoskeletal/contractile system in D. discoideum amoebae.
    Zusätzliches Material: 14 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 55
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 343-354 
    ISSN: 0886-1544
    Schlagwort(e): NBD-phallacidin ; actin ; ocular tissues ; wound repair ; stress fibers ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The fluorescent derivative of the actin-binding toxin phallacidin, 7-nitrobenz-2-oxa-1,3 diazole phallacidin, has been used to cytologically demonstrate the presence of actin in lens epithelium, corneal endothelium, and retinal pigment epithelium. In these noninjured tissues, no stress fibers are observed and fluorescence is confined mainly to an area at or near the cell membrane, although some diffuse cytoplasmic staining can also be seen. However, following injury to either the lens epithelium or corneal endothelium of rats and frogs, stress fibers are detected, but only in those cells that migrate into the wound area. Cells on the periphery of each tissue do not partake in would repair and thus maintain their normal appearance. After the tissue has regenerated, stress fibers disappear, and those cells involved in the injury response return to their normal morphology.When rabbit corneal endothelium is placed in tissue culture, stress fibers are observed as the cells migrate away from the initial explant. Upon reaching confluency, these cells spread out and each is surrounded by thick actin-containing bands. Furthermore, they exhibit some stress cables within their cytoplasm. This is in contrast to their appearance in vivo where stress fibers are absent and fluorescence is limited to a region near the cell membrane.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 56
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 369-383 
    ISSN: 0886-1544
    Schlagwort(e): motility ; flagella ; cilia ; microtubules ; Gregarines ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The male gametes of the parasitic protozoan, Lecudina tuzetae, have a motile flagellum with a “6 + O” ultrastructure ‘Schrével and Besse, 1975’. These gametes were isolated from the cysts in which they develop and were observed and photographed under a variety of conditions. The flagella beat continuously, without stopping and starting, with a beat period of about 2 sec. They can beat in solutions whose viscosities are greater than 0.5 Nsm-2 (l Nsm-2 = 103 cP). The waveform can be approximated by a series of helical arcs and interconnecting straight regions that travel from the base to the tip. The helical regions have a radius of curvature of 3.2 μm and subtend a final angle of 1.7 radians. The straight portions are 2.0 μm in length. There are two sets of opposing bends, but they do not originate in the same plane. The resulting waveform is an approximately helical coil, with a pitch of 9.8 μm, a pitch angle of 0.6 radian and a peak-to-peak amplitude of 2.3 μm. The sense of the coil is left handed. The axoneme twists during beating. The main differences between the movement of this flagellum and that of typical 9 + 2 flagella are a low beat frequency and three-dimensional bends that produce relatively little forward movement of the cell. Twisting is discussed as a means of discriminating between some types of models of flagellar motility.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 57
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 429-443 
    ISSN: 0886-1544
    Schlagwort(e): 21S dynein ; tubulin ; binding stoichiometry ; ATP sensitivity ; binding cooperativity ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The binding properties of Tetrahymena 21S dynein to doublet A and B subfiber microtubules were analyzed by both a turbidimetric assay (Δ A350 nm) and electron microscopy. KCl-extracted, sucrose-gradient, purified 21S dynein binds to each of the two kinds of axonemal microtubules in both ATP-insensitive and ATP-sensitive modes, even though only a single type of binding occurs to each of the subfibers in situ. Total dynein bound to axonemal microtubules is a composite of binding that is sensitive to dissociation by ATP and binding that is insensitive to ATP. Each exhibits a different binding profile. Total binding exhibits a sigmoid profile (h = 1.93) and saturates at 1.49 mg D/mg T. ATP-sensitive binding likewise exhibits a sigmoid profile (h = 2.66) but saturates at 1.06 mg D/mg T. Binding occurs with a similar affinity for both A and B subfibers. The Hill coefficient (h) for ATP-sensitive binding implies positive cooperativity between binding events. ATP-insensitive binding was studied independently in 20 μM ATP, 10 μM vanadate, which blocks ATP-sensitive binding. ATP-insensitive binding exhibits a hyperbolic profile (h = 1.0) and likewise occurs along each of the two kinds of axonemal tubules. Binding saturates at 0.87 mg D/mg T. The binding data suggest that the tubulin dimer has conserved both ATP-sensitive and ATP-insensitive binding sites for 21S dynein, even though the sites may not be expressed in vivo.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 58
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. i 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 59
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 549-572 
    ISSN: 0886-1544
    Schlagwort(e): flagella ; cilia ; trachea ; microtubules ; crowns ; microtubule assembly ; caps ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The distal tips of the central pair and A-microtubules are capped in mammalian and avian tracheal cilia. The capping structures are similar to those found in protozoan cilia and flagella [Dentler, 1981], and consist of a central microtubule cap that links the central microtubules to the membrane or to the ciliary crown and A-microtubule plugs that insert into the lumen of each of the A-microtubule plugs is bound to the central microtubule cap by distal filaments. The ends of the central and outer doublet microtubules are tightly bound to the cap in both intact and in demembranated and reactivated tracheal cilia. Analysis of the displacement of the microtubule tips in cilia fixed at various bend angles revealed that the displacements of A-microtubules are only partially in agreement with those predicted by the sliding filament model [Satir, 1968]. These results are discussed with respect to the regulation of microtubule sliding in capped cilia and the role of the microtubule capping structures in microtubule assembly.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 60
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 19-24 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 61
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 1 (1981), S. 329-347 
    ISSN: 0886-1544
    Schlagwort(e): actin ; microfilaments ; heavy meromyosin ; mammary gland ; secretion ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Cytochalasin B, a microfilament-altering drug, inhibits lactose synthesis in lactating guinea pig mammary gland [Biochim. Biophys. Acta 392:20, 1975] but not primarily by inhibiting glucose transport [Eur. J. Cell Biol. 20:150, 1979]. In order to study the possible role of microfilaments in lactose synthesis and secretion, we isolated both the alveolar (milk-secreting) and myoepithelial (contractile) cells from lactating mammary gland. Light microscopy shows that the alveolar cell fraction (viability approximately 71%) is homogenous and that the cells retain strong polarity of secretory structures in the apical region. Two proteins were extracted from the alveolar cell fraction. One (mol wt 42,000) comigrates with skeletal muscle actin on SDS-PAGE gels. The other, a high-molecular-weight (180,000) protein (HMWP) may be analogous to actin-binding protein or clathrin. An extract from the myoepithelial cell fraction also contains a protein that comigrates with actin but no HMWP. Whole tissue extract contains the 42K protein, and a 185K HMWP. Examination of the alveolar cell extract by electron microscopic (EM) negative staining revealed meshworks of multistranded, interconnecting filaments, with attached globular structures (100-200 A) (possibly the HMWP) and single filaments (40-60 A diameter) branching off. To localize these filamentous structures in situ, whole tissue was glycerinated and incubated with rabbit skeletal muscle heavy meromyosin (HMM). Masses of filaments in myoepithelial cells served as convenient standards for HMM decoration. Decorated filaments have cross-arms or projections, unlike the narrow, smooth filaments of control tissue. Decorated filaments in alveolar cells are located beneath the plasma membrane, in close association with secretory vacuoles, and near the Golgi apparatus; filaments near the latter two are often oriented perpendicular to the plasma membrane. Microvesicles are embedded in meshworks under the plasmalemma and near the Golgi apparatus. Intermediate-sized (85-115 A diameter), non-decorated filaments diverge from the meshworks of decorated filaments. Microvesicles are associated with intermediate-sized filaments as well. The association of actin-like filaments with secretory vacuoles and microvesicles and their location in areas of the cell concerned with biosynthetic activities suggest a possible function in the intracellular transport of secretory products.
    Zusätzliches Material: 13 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 62
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 165-168 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 63
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 199-204 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 64
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 71-75 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 65
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 93-103 
    ISSN: 0886-1544
    Schlagwort(e): bacterial motility ; flagella ; sheathed flagella ; complex flagella ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Although bacterial flagellar sheaths were observed over 30 years ago, they may still be characterized as structures in search of a function. In addition to true sheaths, bacterial flagella may possess other adornments that cause an increase in the organelle's cross-sectional diameter. These “complex flagella” are sharply differentiated from sheathed flagella. Immunological and chemical distinctions have been found between flagellar sheaths, flagellar cores, and LPS layers inferred to be the sheath sensu stricto. Although complex flagella may serve as specific receptors for flagellotropic phages or in allowing for more efficient swimming in viscous environments, similar functions have not yet been attributed to true sheaths. It is postulated that flagellar sheaths may allow for specific interaction between a bacterium and a surface. In addition, there is a problem as to the relationship between a rapidly rotating flagellum and the sheath.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 66
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 113-121 
    ISSN: 0886-1544
    Schlagwort(e): coelomocytes ; filopodia ; whole cell translocation ; video microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: We have utilized a video-enhanced contrast system coupled to a DIC-equipped microscope to examine the motility of both whole coelomocytes and individual filopodia. When the cells are left in diluted coelomic fluid, they exhibit a fibroblast-like mode of translocation across the substrate. These cells extend lamellipodia at their advancing margin and develop retraction fibers at the trailing edge. Filopodia are actively extended from the lamellipodia of the advancing margin. Cells that are washed free of the coelomic fluid and placed in an isotonic buffer lose their ability to translocate. Filopodia on these stationary cells are seen to undergo a series of waving and bending motions. These motions are rapid and result in a filopodium folding back upon itself only to reextend later. Both forms of motility are discussed in light of the existing structural and biochemical knowledge of this and other cell types.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 67
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 1 (1981), S. 387-397 
    ISSN: 0886-1544
    Schlagwort(e): birefringence ; polarizing microscope ; sea urchin egg ; cortex ; mitosis ; cleavage ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Birefringence (BR) at the cell surface of fertilized eggs of the sand-dollar, Clypeaster japonicus, during mitosis and cleavage was determined with a photoelectric BR detection apparatus [Hiramoto et al, 1981a]. The cortex of about 2 μm thickness is birefringent positive with respect to the normal to the cell surface. The hyaline layer is negatively birefringent. The halo-layer consisting of a row of microvilli surrounding the egg is positively birefringent in normal Ca-free sea water, while it is negatively birefringent in Ca-free sea water with high refractive index. The BR of the cortex gradually increases over the entire surface during mitosis until the onset of cleavage. The BR of the cortex at the polar region reaches a maximum shortly after the onset of cleavage and then decreases, while the BR of the cortex at the equatorial region begins to decrease shortly before the onset of cleavage, reaches a minimum shortly after the cleavage starts, and then increases again as the cleavage furrow advances. The coefficient of birefringence of the cortex is about 2.5 × 10-5 at the maximum. The BR change of the cortex during mitosis and cleavage is interpreted as a passive deformation caused by the constriction of the contractile ring as well as an active structural change of the cortex occurring in the dividing cell.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
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  • 68
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 213-226 
    ISSN: 0886-1544
    Schlagwort(e): microtubules ; fertilization ; cell division ; sea urchin ; cytoskeleton ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The microtubule-containing structures that appear in eggs during fertilization and cell division in the sea urchins Lytechinus variegatus and Arbacia punctulata were detected by antitubulin immunofluorescence microscopy of detergent extracted cytoskeletal preparations. The extraction buffer, which is composed of 0.55 mM MgCl2, 10 mM EGTA, 25 mM MES, 25% glycerol, 1% Nonidet P-40, and 25 μM PMSF, pH 6.7, allows for dramatically improved fluorescent images compared to those obtained using conventional staining procedures, with residual background staining being reduced to near zero.The immunofluorescent images obtained using this technique provide information on several motile events that occur during the first cell cycle. This technique demonstrates that all of the cytoplasmic microtubules are associated with the incorporated sperm's centrioles during female pronuclear migration. This changes during the centration of the male and female pronuclei at which time a monastral array of microtubules forms in the egg's cytoplasm. A large proportion of the monastral microtubules do not appear to be associated with the centrioles. At prophase and early metaphase, the centrioles are the dominant microtubule organizing centers (MTOCs) consistent with mitotic theories that the kinetochore catches, but does not initiate, microtubules. Observations of intercentriolar distances show that there are three stages of pole separation during the first cell cycle. The initial separation occurs during pronuclear centration, the second during the streak stage, and the final one during the late stages of mitosis. At telophase, polar microtubules appear to extend into the cortex supporting the cell surface at all regions except the presumptive cleavage site.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
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  • 69
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983) 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 70
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 273-280 
    ISSN: 0886-1544
    Schlagwort(e): Chlamydomonas flagellar collars ; Chlamydomonas cell wall ; mating in Chlamydomonas ; cell wall proteins ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The flagella of Chlamydomonas reinhardtii protrude through the cell wall via short, tunnel-like openings that are lined with 11 nm × 500 nm fibers arranged in parallel array. These cylindrical collections of fibers presumably permit free movement of the flagella within the cell wall. In this report electron-microscopic evidence is presented showing that during the initial stages of the mating reaction intact collars slip off of the ends of the flagella when cell wall loss occurs. Electrophoretic analysis of isolated collars reveals one major protein and several minor species.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 71
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 333-347 
    ISSN: 0886-1544
    Schlagwort(e): Caenorhabditis elegans spermatozoa ; cell motility ; electron microscopy ; cell-substrate contact ; 2-nm filaments ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The locomotion of C. elegans spermatozoa resembles, in many respects, the crawling movements of other eukaryotic cells. However, these sperm contain surprising little actin, which plays no apparent role in this cell's motility. Electron microscopy has revealed that crawling spermatozoa retain a strict morphological polarity so that the organelle-filled cell body is separated from the pseudopod by an array of cytoplasmic laminar membranes. When sperm crawl only the pseudopod contacts the substrate; the cell body is either pulled behind or carried on top of the rear portion of the pseudopod. Fingerlike projections which extend forward from the leading edge of the pseudopod initiate contact with the substrate. The underside of the pseudopod exhibits areas of close (40 nm separation) membrane-substrate association with intervening areas of wide (up to 300 nm) membrane-substrate gaps. The pseudopod cytoplasm contains 2-nm filaments but no filamentous actin has been observed. These 2-nm filaments were detected in thin sections of crawling cells and in negative-stained remnants of spermatozoa disrupted by either hypotonic buffer on Triton X-100. The filaments are found both free in the cytoplasm and closely associated with the cytoplasmic face of the plasma membrane and are usually oriented along the long axis of the cell. Neither the identity nor the function of these filaments has been established although their location and orientation suggest that they may be involved in generating propulsion.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
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  • 72
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 349-361 
    ISSN: 0886-1544
    Schlagwort(e): myosin phosphorylation ; actin polymerization ; chemotactic factors ; leukocytes ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Changes in the state of polymerization of actin and phosphorylation of myosin have been observed in polymorphonuclear leukocytes (PMNs) soon after the addition of the chemotactic peptide N-formylnorleucylleucylphenylalanine. At a time when the cells are observed to extend many ruffles or lamellipodia from their surface, the fraction of the cellular actin present in a monomeric form is decreased by about 25% as assayed by the ability of the G-actin to inhibit DNAase. These changes are temporally correlated with an increase in the staining by nitrobenzooxadiazole (NBD)-phallacidin, a probe that binds F-actin selectively. The NBD-phallacidin staining is observed in the surface ruffles. When the peptide concentration is decreased by addition of a tenfold excess of buffer, cells withdraw their surface ruffles and form blebs. These changes correlate with an increase in the G-actin levels detected with the DNAase inhibition assay. An increase in phosphorylation of the 20,000-dalton light chain of myosin is also observed in leukocytes stimulated by addition of chemotactic peptide. These observations of changes in cytoskeletal proteins of PMNs provide a beginning for further studies on the regulation of cell motility by chemotactic factors.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 73
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 391-397 
    ISSN: 0886-1544
    Schlagwort(e): focal contacts ; microfilaments ; microinjection ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The role of structural elements in the organization and maintenance of focal contacts was studied by microinjecting into tissue culture cells specific probes which interfere with filamentous actin or with vinculin: actin interaction. Injection of actin capping proteins from Physarum and brain resulted in breakdown of microfilament bundles starting at their distal ends and in loss of focal contacts. This process was fully reversible. Injection of a high affinity antibody against chicken gizzard vinculin led to partial breakdown of microfilament bundles concomitant with disruption of focal contacts with vinculin remaining at the plasma membrane. This process was irreversible.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 74
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 431-438 
    ISSN: 0886-1544
    Schlagwort(e): myotendinous junction ; laminin ; type IV collagen ; heparan sulfate proteoglycan ; alpha actinin ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The muscle-tendon junction of murine skeletal muscles has been analyzed by a variety of extraction techniques, by myosin subfragment-1 binding experiments, and by ultrastructural immunocytochemistry. The results indicate that the muscle-tendon junction is composed of four distinct domains: an intracellular domain, the internal lamina; a domain connecting the internal lamina with the lamina densa of the external lamina, the connecting domain; the lamina densa; and a domain which attaches the lamina densa to the collagen fibers, the matrix. Each of these domains is distinct with respect to position, three-dimensional organization, and molecular composition, and is therefore considered to have a unique role in the transmission of contractile force.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 75
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 463-483 
    ISSN: 0886-1544
    Schlagwort(e): intracellular matrix ; extracellular matrix ; covalently cross-linked matrix ; ε-(γ-glutamic) lysine bonds ; skeletal muscle ; titin ; covalently cross-linked collagen ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: When skeletal, cardiac, and smooth muscle is exhaustively extracted with a protein-unfolding reagent such as 6 M guanidine HCl and a disulfide-reducing reagent such as 5% β-mercaptoethanol, a tissue ghost remains intact and retains the characteristic shape and dimensions of the tissue before extraction. In the case of chicken pectoral muscle, the tissue ghost contains 1% of the original muscle proteins. Guanidine HCl extraction followed by collagenase treatment of glycerol-extracted chicken pectoral muscle releases a clean preparation of elongated structures containing 0.2% of the original protein and representing the covalently cross-linked remnants of the muscle fibers. The material of these muscle fiber ghosts extends throughout the interior of the cell. Antibodies raised against the tissue ghosts of smooth muscle cross-react with glycerol extracted skeletal myofibrils, forming a banding pattern which coincides with the banding pattern observed when myofibrils are reacted with antibodies against titin. Titin, a large and soluble protein found in skeletal muscle, cross-reacts with our antigizzard antibody. However, amino acid analysis of the muscle fiber ghosts indicates that titin cannot be the only subunit of the insoluble polymer, but that one or more proteins with a very high glycine and alanine content and a very low basic and acidic amino acid content must also form part of the covalently cross-linked matrix. The possibility is presented that this matrix may be the basis of the superthin 2-3-nm filaments which have been observed in a variety of cell types.
    Zusätzliches Material: 12 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 76
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 513-524 
    ISSN: 0886-1544
    Schlagwort(e): fertilization ; actin ; microfilaments ; sea urchin ; cell division ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The sea urchin egg at fertilization is an ideal model in which to study actin-mediated surface activity. Electron microscopy of unfertilized eggs demonstrates the presence of thousands of well-arrayed short microvilli, which appear supported by cytochalasin-sensitive actin oligomers as detected with rhodamine-labeled phalloidin staining of permeabilized eggs. At insemination, the previously short microvilli elongate and cluster around the successful sperm during incorporation. Phalloidin staining demonstrates a tremendous recruitement of polymerized actin into the site of sperm incorporation, resulting in the formation of the fertilization cone. Fertilization of cytochalasin-treated eggs results in the normal activation of the metabolic and bioeletric events, but sperm incorporation does not occur since the localized actin assembly required for fertilization cone formation is precluded. After sperm incorporation, the entire fertilized surface is restructured, as a result of a massive polymerization of actin to produce a burst in microvillar elongation. Addition of cytochalasin to eggs immediately following sperm incorporation demonstrates the recruitment of actin assembly for the proper progression through the first cell cycle. During normal cell divison, the egg surface retains the long microvilli. The furrow which forms at cytokinesis does not appear as a unique new structure, but rather as a reorganization of the cortical microfilaments. Quantitative fluorescence microscopy argues against an increase in microfilaments during early cytokinesis. At the latest stages of cytokinesis, a thickening of the cortical actin is noted, which could possibly be interpreted as a contractile ring. A minor basal level of actin assembly with numerous nucleation sites in unfertilized eggs and a tremendous but localized assembly of microfilaments surrounding the sperm during incorporation, followed by a massive global microfilament assembly event to elongate the fertilized egg microvilli resulting later in the reorganization of these microfilaments to produce the forces necessary for cytokinesis, highlight the utility of the study of sea urchin eggs at fertilization for understanding actin-membrane interactions.
    Zusätzliches Material: 13 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 77
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 553-565 
    ISSN: 0886-1544
    Schlagwort(e): microfilaments ; cytoskeleton ; simian virus 40 ; cell adhesion ; cell surface ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: In order to assess the role of cytoskeletal structure in modulating cell surface topography during cell transformation, cytoskeletal organization of 3T3 mouse cells transformed with a tsA mutant of simian virus 40 (SV40) was studied in detail by correlative light and electron microscopy. Detergent-extracted, criticalpoint dried whole cells observed in the electron microscope were seen to contain well-organized microfilament bundles (stress fibers) traversing the longitudinal axis of cells grown at the restrictive temperature (39°C). When grown at the permissive temperature (32°C), cells prepared in this manner were not observed to contain such structures. However, when semithin sections (0.5 μm) were viewed by transmission electron microscopy at 120 kV, short microfilament bundles were seen in 32°C-grown cells. There was an alteration in the morphology of these structures at sites of attachment to the substratum (focal contacts), and they were shorter in length than microfilament bundles of 39°C-grown cells. A difference was also observed between the two phenotypes in the layer of microfilaments associated with the dorsal cell surface. Since it is this layer that directly determines cell surface architecture, it is proposed that changes in microfilament bundle-generated surface tension are responsible for alterations of this layer, leading to an altered cell surface morphology. Tension may be modified by disturbances in focal contacts (or adjacent regions) or altered actin-associated protein(s).
    Zusätzliches Material: 12 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 78
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 609-622 
    ISSN: 0886-1544
    Schlagwort(e): erythrocyte membrane ; surface elastic shear modulus ; membrane viscosity ; hereditary disorders of blood ; membrane yield ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Measurements of the mechanical properties of the erythrocyte membrane provide a direct assessment of the proper function of its structural components. To assess the effects of alterations in molecular structure on membrane mechanical properties, measurements have been performed on cells from six individuals whose membranes contain inherited, biochemically characterized structural defects. Because the contribution of the memmbrane skeleton to the mechanical behavior of the membrane is most evident in shear deformation, mechanical experiments were performed to measure the material constants which characterize the response of the membrane to shear force resultants. The surface elastic shear modulus characterizes the elastic response of the membrane; the yield shear resultant is the maximum shear force resultant which the membrane can support elastically; and the plastic viscosity coefficient characterizes the rate of membrane deformation when the elastic limit has been exceeded.Generally, it was found that when the molecular defect is found to occur in a region of the skeleton which is stress-supporting, the maximum elastic strength of the membrane is reduced. However, the magnitude of the reduction can be quite different for membranes having similar or even identical defects. In some cases the differences can be attributed to the removal of the most fragile cells of the population by the spleen, but other results indicate that the biochemical description of the defects may be incomplete. These results emphasize the need for further refinements both in the biochemical characterization of membrane skeleton structure and in the description and measurement of membrane mechanical properties.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 79
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 4 (1984), S. 129-135 
    ISSN: 0886-1544
    Schlagwort(e): amoeboid motion ; chemoattractants ; chemotaxis ; Dictyostelium ; filopodia ; folic acid ; pterins ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Living vegetative D. discoideum amoebae were studied to determine whether their filopodia respond to folic acid, a chemoattractant for these cells. Exponentially growing amoebae (ca. 10 μm diameter) exhibit 5-30 μm long filopodia; at stationary phase, aggregation competent amoebae have numerous multibranched filopodia up to 100 μm long. Folic acid was observed to stimulate production, elongation, and branching of filopodia with its effects progressively changing as the amoebae approach aggregation. Filopodial construction was also found to be dependent upon Mg2+ levels. The significance of these results is discussed with respect to progressive changes within the vegetative phase as well as to the mechanisms of amoeboid movement, pseudopodial activity, and chemotaxis.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 80
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 649-655 
    ISSN: 0886-1544
    Schlagwort(e): axonal transport ; lymphocyte capping ; spectrin ; fodrin ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Fodrin is an actin/calmodulin-binding protein with similarities to spectrin (erythrocytes) and TW 260/240 (brush border). It is concentrated beneath the plasma membranes of neurons and other cells. We have observed translocations of fodrin in both neurons and lymphocytes. Newly synthesized, radiolabeled fodrin moves down axons at a maximum velocity (about 50 mm/day) that is slower than the most rapidly axonally transported proteins (group I). A portion of fodrin appears to move more slowly at velocities (1-10 mm/day) resembling those of actin and myosin (group IV) and tubulin and neurofilament proteins (group V). In lymphocytes, when certain surface antigens are induced by cross-linking agents to migrate to one pole of the cell and form a cap, fodrin redistributes beneath the membrane and forms a subcap. The movements of fodrin in lympohocyte capping and in the axonal transport of group IV polypeptides have certain similarities. In both cases, the redistribution of fodrin is accompanied by concomitant redistributions of actin, myosin, and calmodulin, and both processes proceed at similar velocities. We consider the possibilities that these two processes are related, both being driven by a submembrane force-generating system comprising in part actin, myosin, and fodrin, and that fodrin serves to link various organelles or proteins to this system.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 81
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 4 (1984), S. 1-5 
    ISSN: 0886-1544
    Schlagwort(e): motility ; power output ; muscle ; flagella ; cytokinetic furrow ; mitotic spindle ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Cellular motile systems as diverse as muscle and the mitotic spindle have been compared by their specific power output: the maximum power they develop per unit of engine volume. Striated muscles and flagella have high specific output; their performance is comparable to that of typical automobile engines. The cytokinetic furrow and the mitotic spindle have very much lower specific power output. The furrow's output is 7,000 times lower than muscle and the spindle's is 300,000 times lower. Different macromolecules have been used to generate power in systems with similar output (muscles and flagella) and, conversely, the same macromolecular motor has been used in systems with very different output (muscles and cytokinetic furrows). The common feature amid this diversity is adaptation to a particular biological role, which specific power output reflects very well. High values are found where a powerful, compact engine should be advantageous, while low values are found where precision, not power, matters most.
    Zusätzliches Material: 2 Tab.
    Materialart: Digitale Medien
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  • 82
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 4 (1984), S. 76-76 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 83
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 73-82 
    ISSN: 0886-1544
    Schlagwort(e): desmids ; videomicrography ; photokinesis ; photophobic response ; photophosphorylation ; photosynthesis ; phototaxis ; uncouplers ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The effects of the uncouplers CCCP and DNP on photokinesis, phototaxis, and the photophobic response in the desmid Cosmarium have been studied both in population systems and by videomicrographic, single-cell analysis. Light-dependent motility is specifically inhibited by both uncouplers, indicating that photokinesis is driven by photophosphorylation. In population experiments, phototaxis and accumulations in light traps due to photophobic responses are inhibited by drug concentrations comparable to those that inhibit photokinesis. Analysis of single-cell behavior demonstrated, however, that neither photophobic responses elicited by an increase in light intensity (step-up response) nor by a decrease (stepdown response) are inhibited, as long as the reduced motility allows the organisms to cross a light--dark border. Phototactic orientation is not impaired by DNP in the single cell analysis, but CCCP significantly reduced the degree of orientation. The results indicate that, although chlorophyll is the photoreceptor for all three photoresponses, at least the photophobic response is independent of both the photosynthetic electron transport chain and photophosphorylation.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
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  • 84
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 91-92 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 85
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982) 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 86
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 93-101 
    ISSN: 0886-1544
    Schlagwort(e): cell motility ; singlet microtubules ; dynein ; coccid insect ; aflagellate spermatozoa ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: In this report we demonstrate that in the coccid insects Pseudococcus, Phenacoccus, and Planococcus the whole spermatozoon is made up by a nuclear central core surrounded by two complete and one incomplete turns of concentric microtubule palisades. Microtubules of the outer row are linked by a system of short projections 6 nm long; those of the inner row are linked to each other by similar arms; a second system of 6 nm arms links the tubules of each inner row to those of the respective outer row. All these systems of arms are longitudinally spaced every ∼ 12 nm. The motility of this spermatozoon is due to waves progressing from the posterior extremity to the anterior one. By SDS polyacrylamide gel electrophoresis a group of high molecular weight polypeptides is detected, one of which migrates in coincidence with the A dynein band from sea urchin sperm. Our data suggest that occurrence in coccid spermatozoon of a motility due to singlet tubules-dynein interaction.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
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  • 87
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 103-113 
    ISSN: 0886-1544
    Schlagwort(e): actin ; cleavage ; fluorescein-labeled phalloidin ; microinjection ; phalloidin ; sand dollar eggs ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Effects of microinjection of phalloidin on fertilization and cleavage of sand dollar (Clypeaster japonicus and Scaphechinus mirabilis) eggs were studied. The drug, previously injected into unfertilized eggs, showed no effect on the elevation of the fertilization membrane upon insemination up to an intracellular concentration of 50 μM. However, the movement of the egg pronucleus to the sperm pronucleus was inhibited and the fusion of pronuclei did not occur. The subsequent development no longer took place. When phalloidin was injected into fertilized eggs, the thickness of the cortical layer increased and the microvilli became conspicuous. Both nuclear division and cleavage were inhibited at the intracellular concentration of more than 20 μM, though the latter seemed to be more sensitive to phalloidin than the former.Fluorescein-labeled phalloidin (FL-phalloidin) was injected into eggs in order to investigate F-actin localization by fluorescence microscopy. In both unfertilized and fertilized eggs, FL-phalloidin was localized in the cortical layer within 1 min after injection. It was also localized in the cortical layer as radially oriented rodlike structures when injected into fertilized eggs before the disappearance of the nuclear membrane. No distinct fluorescence was detected in the mitotic apparatus or in the cleavage furrow. FL-phalloidin redistributed gradually into egg cytoplasm. In unfertilized eggs, fluorescent rods were found especially in the egg pronucleus 30 min after injection.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 88
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 131-147 
    ISSN: 0886-1544
    Schlagwort(e): higher land plant contractile system ; actin activation of myosin ; S-1 decoration of actin ; polymerization of actin ; calcium sensitivity of actomyosin interaction ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: This paper describes the initial isolation of actin- and myosin-like proteins from the cytoplasm of the endocarp tissue cells of the fruit of the tomato, Lycopersicon esculentum. Low ionic strength buffers extracted the 42,000 molecular weight tomato actin in the depolymerized form. Tomato actin can be polymerized in 0.1 M KCl, 2 mM MgCl2 to form 6 nm diameter filaments resembling rabbit skeletal muscle F-actin in their ultrastructure and pattern of decoration with rabbit myosin subfragment-1 (S-1). Tomato F-actin activates the low ionic strength Mg2+ ATPase of rabbit S-1 up to ten-fold. High ionic strength extracts of tomato yield a myosinlike enzyme whose ATPase activity in 0.5 M KCl is maximal in the presence of K+-EDTA and is repressed in the presence of Mg2+. The column-purified enzyme forms a complex with rabbit F-actin, which can be dissociated by Mg2+ ATP. The low ionic strength Mg2+ ATPase of tomato myosin can be activated ten-fold by rabbit actin and up to nineteen-fold by tomato actin. No activation of the tomato myosin by rabbit F-actin occurs in the absence of free calcium ions.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 89
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 149-161 
    ISSN: 0886-1544
    Schlagwort(e): cell locomotion ; gastrulation ; contact paralysis ; collagen substratum ; serum factors ; morphogenetic movements ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Prospective mesodermal cells of Xenopus laevis gastrulae showed substantial locomotion in vitro, averaging 4.3 μM/min, when dissociated and cultured on a glass surface coated with collagen and fetal bovine serum. The cell translocate by making lamellipodia and filopodia whereas the main cell body remains rounded. When two mesodermal cells made contact with each other, they showed contact paralysis of lamellipodial activity. In contrast, when mesodermal cells contact ectodermal cells, contact paralysis does not occur. Rather, migrating mesodermal cells continue to translocate. The locomotion in vitro appears to mimic that in vivo during gastrulation, because of the similarities of the rate of movement and the cell shape in culture and in embryos. Neither prospective ectodermal cells from gastrulae nor prospective mesodermal cells from blastulae showed locomotion under the same culture conditions.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 90
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 191-194 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 91
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 167-184 
    ISSN: 0886-1544
    Schlagwort(e): saltatory organelle movements ; ciliary movement ; dynein ; vanadate ; microinjection ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: To test the idea that saltatory organelle movements of nonmuscle cells might be driven by microtubule-dynein interactions, we microinjected vanadate into several different types of cultured cell. Solutions of sodium metavanadate made up in a simple buffered salt solution were pressure microinjected into fully spread cells in an open-topped culture chamber placed on the stage of an inverted microscope. The cells were observed by oil-immersion phase-contrast optics and results were recorded on movie film. Vanadate, at 10-5-10-2 M, microinjected into cultured chick embryo fibroblasts, failed to inhibit organelle movements. To test the effectiveness of vanadate's inhibitory action under living cell conditions, ciliated epithelial cells were micro-injected. In these cells even the smallest microinjection of 5 × 10-5 M vanadate caused an immediate cessation of ciliary beating. Moreover, in cells that were well spread it was found that whereas vanadate, at 5 × 10-5 × 10-3M, inhibited ciliary motion, it failed to inhibit organelle saltations in the same cell. To determine whether vanadate would inhibit a living actin-myosin system, myocardial cells were also microinjected. Following microinjection of 5 × 10-5 and 5 × 10-4M vanadate a temporary tonic contraction (which also occurred following microinjection of buffer alone) was followed by regular beating. Taken together these results demonstrate that in living cell systems microtubule-dynein interactions are as sensitive to vanadate inhibition as they are in demembranated model systems, and that a working actin-myosin system in a living muscle cell does not share this great sensitivity. In light of the pronounced differential inhibitory effects of vanadate on the movements of cilia and organelles, our results suggest that saltatory organelle movements in chick embryo fibroblasts and rabbit oviduct epithelial cells are unlikely to be brought about by microtubule-dynein interactions.
    Zusätzliches Material: 22 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 92
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983) 
    ISSN: 0886-1544
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 93
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 247-259 
    ISSN: 0886-1544
    Schlagwort(e): spermatozoa ; Ciona ; axoneme ; quiescence ; twist ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: A simple planar model of sliding can predict the amount of sliding required to form a certain degree of bend. The accuracy of this prediction relies on the assumptions that no twists occur in the axoneme and that no sliding occurs at the base. However, previous studies indicated that twists may occur.This paper explores a new method for quantitating and analyzing twists. Preliminary results using this method showed that there were twists. In order to control for possible artifacts due to fixation and other preparative procedures, the characteristic S-shaped quiescent state of Ciona spermatozoa was studied.Analyses of platinum replicas of those flagella in which this waveform is well preserved suggest that most, if not all, of the twists observed are due to the artifact of a curved shape settling onto a surface. Detailed analyses indicate that if twists do occur in quiescent sperm, they are probably less than 0.4 radian. Since axonemes are evidently easily twisted in rigor, and even after fixation, caution should be exercised in interpretation of axonemal twists.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 94
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 261-271 
    ISSN: 0886-1544
    Schlagwort(e): chromosome movement ; meiosis ; spermatocytes ; prophase ; nuclear envelope ; aster ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Association of bivalent chromosomes with the astral centers and nuclear envelope was analyzed in crane-fly spermatocytes during the final hours of diakinesis. In contrast to other systems in which movement of chromosomes during diakinesis correlates with the clustering of bivalents near the astral centers, such clustering is not prevalent in crane-fly spermatocytes. Polarization indices of bivalents calculated 5 to 10 minutes before the end of diakinesis provided evidence for polarization of only a fraction of all bivalents. Similar results were obtained in a large number of fixed cells in which asters and chromosomes were preferentially stained. Ultrastructural analysis of cells in late diakinesis revealed significant contact between bivalents and the nuclear envelope in all 46 cells that were analyzed. The extent of contact in some cells was greater than in others. Sites of contact included the telomeric ends of bivalents, and in some cases the distribution of contact sites suggested the possible involvement of centromeres in chromosome-nuclear envelope association. The results are consistent with the hypothesis that a dynamic interaction between chromosomes and nuclear envelope may exist during late prophase, when the movement of chromosomes is known to occur.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 95
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 283-305 
    ISSN: 0886-1544
    Schlagwort(e): taxol ; microtubules ; intermediate filaments ; fibroblasts ; epithelial cells ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Taxol promotes microtubule (MT) assembly in vitro and induces the reorganization of the cytoskeleton into unusual MT arrays in cultured cells. The possibility that taxol also has an indirect effect on intermediate filaments (IF) was investigated. In baby hamster kidney (BHK-21) and human skin (ENSON) fibroblasts treated with 1-10 μM taxol for 1-24 h, the drug induces changes which are similar to those produced by colchicine. These include a loss of major cellular extensions, a redistribution of organelles to a perinuclear location, and an inhibition of locomotion. Saltatory particle movements are not inhibited, however. Ruffling and filopod formation continue, indicating that cells are viable up to 24 h.Polarized light microscopy of living fibroblasts treated with taxol reveals the presence of perinuclear birefringent material which has been examined by immunofluorescence. In control cells, IF and MT radiate from a juxtanuclear region and extend to the cell periphery. In taxol-treated cells, MT and IF are excluded from cell margins, forming large central bundles.In the epithelial cell lines PtK2 and PAM, the keratin system of IF does not become redistributed; in PtK2, however, a second fibroblastlike system of IF does become redistributed to a perinuclear position during taxol treatment.Ultrastructural analyses show that taxol-treated fibroblasts contain parallel arrays of cross-bridged MT-IF as well as bundles of MT exclusive of IF. Epithelial cells contain a predominance of IF-free MT bundles which are organized into hexagonally packed arrays. In these bundles MT frequently exhibit hooks or other incomplete MT profiles and are linked by filamentous material.
    Zusätzliches Material: 14 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 96
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 375-382 
    ISSN: 0886-1544
    Schlagwort(e): actin ; spectrin ; band 4.1 ; cytochalasins ; erythrocyte ; brain ; actin-membrane attachment ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: A complex of proteins with properties similar to those of erythrocyte spectrinband 4.1-actin complex has been idientified in a preparation derived from bovine brain. The complex has an apparent sedimentation coefficient of about 26S, and contains brain spectrin (also called fodrin) and actin as major components. The actin in the complex is in the oligomeric form, which nucleates assembly of actin filaments that grow from the “barbed” end. The complex cross-links actin filaments, resulting in an increase in low-shear viscosity. Whether the complex contains a protein analogous to erythrocyte band 4.1 is not known. However, it can be demonstrated that brain spectrin has the capability to interact with band 4.1 in a way which increases its ability to cross-link actin filaments.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 97
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 405-417 
    ISSN: 0886-1544
    Schlagwort(e): vinculin ; focal contacts ; microfilaments ; transformation ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Talin is a recently identified cytoskeletal protein with a polypeptide molecular weight of 215,000 daltons. In cultured fibroblasts talin has been localized by immunofluorescence in adhesion plaques (focal contacts), in the ruffling membranes and leading lamellae of the cell periphery, and in fibrillar patterns that align with microfilament bundles and/or with cell surface fibronectin. These cellular locations suggest that the protein could function either in the attachment of microfilaments to the plasma membane or in the organization of microfilaments close to membrane attachment sites. Cell transformation by viruses such as Rous sarcoma virus disrupts the normal organization of talin, and in most transformed cells talin appears distributed diffusely through the cytoplasm. In a few cells talin is detected in doughnut-shaped aggregates, as a ring surrounding a central core of actin. The significance of these structures is uncertain, but in some cells the individual structures will condense to form much larger aggregates with a striking appearance when viewed by immunofluoresence microscopy.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 98
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 579-588 
    ISSN: 0886-1544
    Schlagwort(e): calcium-dependent protease ; contractile proteins ; platelets ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 99
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 623-633 
    ISSN: 0886-1544
    Schlagwort(e): spectrin ; ankyrin ; brain membranes ; spectrin subunits ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Further similarity between mammalian erythrocyte spectrin and pig brain spectrin has been demonstrated by (a) formation of hybrid molecules with brain α-chains and erythrocyte β-chains and by (b) identification of an ankyrin protein in brain membranes. Hybrid spectrin molecules prepared from brain α-chains and erythrocyte β-chains were visualized by low-angle rotary shadowing as double-stranded rods (dimers) 100 nM in length. 125I-labeled brain α-chain that was hybridized with erythrocyte β-subunit acquired ability to bind to ankyrin sites on erythrocyte membranes. 125I-labeled brain α-chain bound only to β-subunits of erythrocyte and brain spectrin following transfer of these polypeptides to nitrocellulose paper from sodium dodecyl sulfate (SDS) gels. Thus brain spectrin and mammalian erythrocyte spectrin have shared functional sites involved in association of their subunits. Additional evidence for similarity of brain and erythrocyte membranes is the finding of a 210,000 Mr membrane protein in brain that cross-reacts with erythrocyte ankyrin and has a water-soluble domain of 72,000 Mr that is produced by protease digestion. The 72,000 Mr domain of brain ankyrin has been isolated by affinity chromatography on erythrocyte spectrin-Sepharose, and was demonstrated to bind directly to erythrocyte and brain spectrin. The brain 72,000 Mr fragment has distinct peptide maps from the erythrocyte 72,000 Mr ankyrin fragment and thus is not a result of erythrocyte contamination.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 100
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 671-682 
    ISSN: 0886-1544
    Schlagwort(e): actin ; cytoskeleton ; membrane connections ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Recently, molecules highly related to erythrocyte spectrin have been identified in nonerythroid cells. Here we summarize our current understanding of these molecules and suggest a model for their organization. Significant differences exist between this family of proteins isolated from mammalian cells and avian cells, and this may explain the variability in antibody preparations as well as differences in peptide maps of these subunits which have been reported. We have prepared antibodies specific for the variant subunits of the spectrinlike proteins fodrin, spectrin, and TW260/240 and analyzed the distribution of these variant subunits in different chicken cell types as well as their developmental distribution in the intestine. The results suggest that fodrin is the general member of this family of proteins and can even coexist with other spectrinlike proteins in the same cells.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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