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  • Wheat  (108)
  • Triticum aestivum  (105)
  • Springer  (195)
  • American Geophysical Union (AGU)
  • Annual Reviews
  • 1980-1984  (195)
  • 1950-1954
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  • Springer  (195)
  • American Geophysical Union (AGU)
  • Annual Reviews
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  • 1
    Electronic Resource
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    Springer
    Theoretical and applied genetics 65 (1983), S. 41-46 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Protein ; Mutation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Poor adaptability or functional quality of much germplasm used for breeding high-protein hard red winter wheats prompted mutagenesis as an alternative means of increasing grain protein content. Four hard red winter wheat genotypes — KS644 (‘Triumph// Concho/Triumph’), ‘Kaw’, ‘Parker’, and ‘Shawnee’ — were treated with 0.40 M ethyl methanesulfonate (EMS). Advanced lines (M8-M10) were selected that had a 3-year mean grain protein advantage of 0.7% to 2.0% over controls. Increased grain protein content was generally associated with decreased grain yield and kernel weight, but some high-protein mutant lines had yields or kernel weights similar to those of original genotypes. Changes in height and lodging induced by EMS were generally favorable, most mutants being shorter and lodging less than controls, but blooming date was generally delayed, a deleterious change. One line also changed from resistant to segregating for wheat soil-borne mosaic virus. Mutant lines might be utilized in cross-breeding programs, particularly if negative pleiotropic effects and linkages are absent.
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  • 2
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    Theoretical and applied genetics 56 (1980), S. 17-23 
    ISSN: 1432-2242
    Keywords: Wheat ; Rye ; Triticale ; Highly repeated ; DNA sequences ; Heterochromatin ; Translocations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using in situ hybridization techniques, we have been able to identify the translocated chromosomes resulting from whole arm interchanges between homoeologous chromosomes of wheat and rye. This was possible because radioactive probes are available which recognize specific sites of highly repeated sequence DNA in either rye or wheat chromosomes. The translocated chromosomes analysed in detail were found in plants from a breeding programme designed to substitute chromosome 2R of rye into commercial wheat cultivars. The distribution of rye highly repeated DNA sequences showed modified chromosomes in which (a) most of the telomeric heterochromatin of the short arm and (b) all of the telomeric heterochromatin of the long arm, had disappeared. Subsequent analyses of these chromosomes assaying for wheat highly repeated DNA sequences showed that in type (a), the entire short arm of 2R had been replaced by the short arm of wheat chromosome 2B and in (b), the long arm of 2R had been replaced by the long arm of 2B. The use of these probes has also allowed us to show that rye heterochromatin has little effect on the pairing of the translocated wheat arm to its wheat homologue during meiosis. We have also characterized the chromosomes resulting from a 1B-1R translocation event. From these results, we suggest that the observed loss of telomeric heterochromatin from rye chromosomes in wheat is commonly due to wheat-rye chromosome translocations.
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  • 3
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    Theoretical and applied genetics 57 (1980), S. 57-58 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Anther culture ; Haploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Five haploid plants of wheat were used for anther culture. Embryos were formed and six plants were regenerated. Of these, two were haploid (n=3x=21) and two diploid (2n=6x=42). The two diploids derived from the anthers of the same haploid wheat plant gave seeds, but the fertility was reduced in one of them showing, abnormalities at meiosis.
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  • 4
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    Theoretical and applied genetics 57 (1980), S. 57-58 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Anther culture ; Haploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Five haploid plants of wheat were used for anther culture. Embryos were formed and six plants were regenerated. Of these, two were haploid (n=3x=21) and two diploid (2n=6x=42). The two diploids derived from the anthers of the same haploid wheat plant gave seeds, but the fertility was reduced in one of them showing, abnormalities at meiosis.
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  • 5
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    Theoretical and applied genetics 58 (1980), S. 107-111 
    ISSN: 1432-2242
    Keywords: Glutenin ; Wheat ; Bread-making ; Chromosomal control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The subunit composition of glutenin from 47 European wheat cultivars was studied using SDS-polyacrylamide gel electrophoresis. These cultivars are genetically related since they originate from the same stock. Moreover, the diversity of sample, containing cultivars with very different French bread-making qualities, makes it possible to investigate the relationship between glutenin subunit composition and bread-making quality. 16 electrophoretic types of glutenin subunits could be distinguished: these were grouped into four classes. Depending on the cultivar, six to eight glutenin subunits with MW more than or equal to 62,000 were detected. Subunits 3 and 5, with an approximate MW of 122,000 and 108,000 respectively, seem to play a prominent role on bread-making quality; they were found in cultivars of good quality and were absent in those unsuitable for making French bread. Two other subunits (9 and 10; MW: 71,000 and 66,000, respectively) have a less defined influence but may be needed in some types of glutenin structure. Aneuploid analysis shows that in ‘Chinese Spring’, subunit 5 is coded by a gene on the long arm of chromosome 1B. The location of genes coding for subunits 3, 9 and 10 could not be determined.
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  • 6
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    Theoretical and applied genetics 59 (1981), S. 25-31 
    ISSN: 1432-2242
    Keywords: Seed proteins ; Wheat ; Barley ; Rye ; Ae. umbellulata ; Ag. elongatum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The seed proteins of ‘Chinese Spring’ wheat stocks which possess single chromosomes from other plant species related to wheat have been separated by gel electrophoresis in the presence of sodium dodecyl sulphate. Marker protein bands have been detected for both arms of barley chromosome 5, chromosome E (= 1R) and B (= 2R) of rye, chromosomes A,B (= 1Cu) and C (= 5Cu) of Aegilops umbellulata and chromosomes I and III of Agropyron elongatum. These studies, and previous findings, indicate that chromosome 5 of barley, chromosome 1R of rye, chromosome I of Ag. elongatum and possibly chromosome 1Cu of Ae. umbellulata are similar to chromosomes 1A, 1B and 1D in hexaploid wheat in that they carry genes controlling prolamins on their short arms and genes controlling high-molecular-weight (apparent molecular weight greater than 86,000) seed protein species on their long arms. These findings support the idea that all these chromosomes are derived from a common ancestral chromosome and that they have maintained their integrity since their derivation from that ancestral chromosome.
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  • 7
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    Theoretical and applied genetics 59 (1981), S. 313-316 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Puccinia graminis ; Stem rust resistance ; Monosomic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using monosomic analysis genes governing resistance in one wheat variety, ‘E5883’, against stem rust races 15C and 122, were located on specific chromosomes. Against race 15C, a major dominant gene, Sr6, was located on chromosome 2D of ‘E5883’ with minor modifiers found on chromosomes 4A and 6D. Against race 122, the presence of a recessive hemizygous ineffective gene, Sr8, was identified, located on chromosome 6A of variety ‘E5883’.
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  • 8
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    Theoretical and applied genetics 59 (1981), S. 317-325 
    ISSN: 1432-2242
    Keywords: Environmental evaluation ; Plant breeding ; Multivariate analysis ; Wheat ; Climatic variable
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A previous attempt to relate long term yields from the Western Australian wheat belt to climatic factors proved only partially successful. Here, principal component analysis has been used to examine the patterns of variability created by those socio-economic factors which may have obscured any underlying relationship which existed between yield and climate. In fact, these analyses revealed the existence of variation peculiar to particular groups of years, a result which could explain why many attempts to relate crop yields directly to climatic factors have proved unsuccessful. The plant breeding implications of these genotype x environment interactions are considered.
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  • 9
    ISSN: 1432-2242
    Keywords: Wheat ; Endosperm ; Proteins ; Electro phoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The major endosperm proteins in a range of genotypes of hexaploid wheat have been fractionated by two-dimensional electrophoresis. The genotypes included nine varieties and forty four intervarietal substitution lines in which chromosomes 1A, 1B, 1D, 6A, 6B or 6D from eight of the varieties have been introduced one at a time into a common genetic background. The appearance of different protein subunits was often correlated with a chromosome substitution. This showed that many of the genes for the high molecular weight protein subunits (molecular weight range 55,000 to 140,000 determined by SDS polyacrylamide gel electrophoresis) are specified by chromosomes 1A, 1B and 1D while many of the lower molecular weight subunits (molecular weight range 30,000 to 45,000) are specified by chromosomes 6A, 6B and 6D. The different protein subunits correlated with chromosome substitution could not always be recognised in the varietal source of the substituted chromosome. The different subunits specified by homologous chromosomes in different wheat varieties may differ in isoelectric point and/or molecular weight.
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  • 10
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    Theoretical and applied genetics 60 (1981), S. 81-84 
    ISSN: 1432-2242
    Keywords: Wheat ; ‘Norin 10’ dwarfing genes ; Photosynthesis ; Respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparative analysis of eight cultivars of spring wheat (Triticum aestivum) classified by height as tall (T), semi-dwarf (D1), dwarf (D2) and very dwarf (D3) was conducted to study their efficiency of oxygen exchange during photosynthesis and dark respiration. Two cultivars were included in each height group. Cultivars carrying ‘Norin 10’ dwarfing genes (D1, D2 and D3) were found to have a significantly higher photosynthetic rate per unit leaf area than talls (T) that lack these genes. Among the ‘Norin’ gene carriers, dwarf group (D2) was most efficient, followed by very dwarf (D3) and semi-dwarf (D1). Photosynthetic rate and respiratory rate were found to have a positive relationship.
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  • 11
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat-phenolics-location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using thin-layer chromatography and nulli-tetrasomic and ditellosomic series of Triticum aestivum L. cv. ‘Chinese Spring’, it has been possible to relate the phenolic compounds found in adult plant leaves and 12 day-old seedling leaves with the chromosomes or chromosome arms 1 B, 2 BL, 3 BL, 5 A, 6 AL, 7 B and 7 DS.
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  • 12
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    Theoretical and applied genetics 61 (1982), S. 337-339 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; ‘Chinese Spring’ ; Chromosome banding ; Hydrolysis ; Feulgen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Terminal and/or interstitial N-bands were produced on the seven B-genome chromosomes and chromosomes 4 and 7 of the A-genome of Triticum aestivum cv. ‘Chinese Spring’ by a modified BSG technique following a standard Feulgen preparation. The banding was accomplished by modifying the barium hydroxide treatment.
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  • 13
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    Theoretical and applied genetics 62 (1982), S. 127-134 
    ISSN: 1432-2242
    Keywords: Wheat ; Genetical analysis ; Cross prediction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetical analysis of the F2 triple test cross design combined with conventional early generations was used to elucidate the genetical control of yield and yield components in two crosses of winter wheat. From estimates of the additive, {d}, and additive X additive, {i}, components of means, together with the additive genetical variance, D, predicted frequencies of recombinant inbred lines that would transgress the parental range were calculated for each cross. The accuracy of predictions was evaluated by comparing expected frequencies with observed numbers in populations of F6 lines previously developed by single seed descent. For both crosses and all characters where an adequate genetical model was found to explain the observed variation between the early generations, good agreement between predicted and observed frequencies of transgressive segregants was obtained. Furthermore, for characters exhibiting significant epistasis, allowance for additive X additive {i} epistasis in the prediction equations was sufficient to allow for skewness of the recombinant inbred population. These results demonstrate that cross performance in wheat can be predicted from genetical analysis of early generations, and the value of this approach in breeding new varieties is discussed.
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  • 14
    ISSN: 1432-2242
    Keywords: Wheat ; Agropyron ; Chromosome pairing ; Alcohol dehydrogenase isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relationships of Agropyron intermedium chromosomes in two wheat-Agropyron addition series were determined. Chromosome pairing behaviour revealed that the alien chromosome in lines TAF-2 and L7 of ‘Vilmorin’-A. intermedium set are homologous to the alien chromosomes in lines P and C of the ‘Caribo’-A. intermedium set respectively. Localization of alcohol dehydrogenase isozyme genes in ‘Vilmorin’-Agropyron addition line L4 and in ‘Caribo’-Agropyron line O indicated relationships with wheat chromosomes of homoeologous group 4.
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  • 15
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    Theoretical and applied genetics 63 (1982), S. 227-233 
    ISSN: 1432-2242
    Keywords: C-banding ; Wheat ; Rye ; Triticale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using different stains, wheat chromosomes could be distinguished from rye chromosomes by preferential staining. C-bands of rye chromosomes were preferentially stained with Giemsa while those of wheat chromosomes were preferentially stained with either Leishman or Wright stain. Preferential staining aids the identification of wheat and rye chromosomes and chromosome segments and in particular the recognition of wheat/rye chromosome substitutions and translocations.
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  • 16
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    Theoretical and applied genetics 64 (1983), S. 103-107 
    ISSN: 1432-2242
    Keywords: Glutenin ; Wheat ; Co-dominance ; Bread-making
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ten pairs of reciprocal crosses have been made between wheat cultivars which show differences in their glutenin subunit compositions. The F1 seed glutenin subunit composition was studied by means of polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS). The results indicate that all the high molecular weight (HMW) and medium molecular weight (MMW) subunits (from 133,000 to 65,000 daltons) are transmitted to the F1 seed generation from the parental cultivars. In accordance with the triploid nature of the heterozygous endosperm (3n) and with the maternal and paternal gene dosage ratio (2∶1) in the endosperm itself, a significant effect of maternal parent is registered when comparing pairs of reciprocal seeds. Genes coding for the glutenin subunits are expressed whatever their doses are (one, two, or three) in the hybrid endosperm; thus the glutenin subunits inheritance is consistent with the co-dominant type. For one pair of the reciprocal crosses, two MMW parental bands (MW: 71,000 and 66,000) seemed absent in the F1 seed patterns while a new band with an intermediate, apparent MW (68,000) appears. This phenomenon was observed when the glutenins analyzed by electrophoresis were previously separated from other endosperm proteins, and not when they were directly extracted from the ground seed. We assume that the extraction can cause interactions between moieties attached to the subunits and lead to the formation of a complex having an intermediate electrophoretic mobility.
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  • 17
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    Theoretical and applied genetics 64 (1983), S. 255-258 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Malate dehydrogenase ; Chromosomal location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The malate dehydrogenase (E.C. no 1.1.1. 37) of Triticum aestivum L. cv. Chinese Spring, shows two activity zones. The results obtained support the hypothesis that the malate dehydrogenase isozymes of zone II are dimers composed of the six possible combinations of subunits coded by triplicate genes located in the long arms of chromosomes of the homoeologous group 1.
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  • 18
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    Theoretical and applied genetics 66 (1983), S. 1-7 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Aegilops species ; Alloplasmic lines ; 2D gel electrophoresis ; Cytoplasmic inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In this first analysis the protein patterns obtained by two-dimensional gel electrophoresis of 8 day-old leaves from 18 alloplasmic wheat lines are compared. From 440 spots retained on the basis of their reproducibility, 36 proteins were observed to vary in different cytoplasms, allowing us to distinguish the T. aestivum cytoplasm from 5 Aegilops cytoplasms. Twenty-four of the 36 variable proteins could be structurally related to the large subunit of RuBPCase. Nuclear variation between 3 wheat varieties was observed for 14 proteins.
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  • 19
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    Theoretical and applied genetics 66 (1983), S. 111-121 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Agropyron ; Intergeneric hybrids ; Embryo culture ; Chromosome pairing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intergeneric hybrids of Triticum aestivum (2n=42,AABBDD) with Agropyron ciliare (2n= 28,SSYY), A. trachycaulum (2n=28,SSHH), A. yezoense (2n=28,SSYY) and A. scirpeum (2n=28) are reported for the first time. F1 hybrids of T. aestivum were also produced with A. intermedium (2n=42,E1E1E2E2Z1Z1) and A. junceum (2n=14,JuJu). All wheat-Agropyron hybrids were obtained by embryo rescue technique. Cultivars and reciprocal crosses differed for seed set, seed development and F1 plant production. The F1 hybrids were sterile. Attempts to obtain amphiploids were unsuccessful. However, backcross derivatives were obtained with wheat as the recurrent parent. The level of chromosome pairing in A. trachycaulum x wheat, A. yezoense x wheat and wheat x A. junceum hybrids provided no evidence of homologous or homoeologous pairing. Mean pairing frequencies in A. ciliare x wheat, wheat x A. scirpeum and wheat x A. intermedium hybrids indicated homoeologous or autosyndetic pairing. Ph gene was more effective in regulating homoeologous pairing in A. yezoense x wheat hybrids than in A. ciliare x wheat hybrid. Chromosome pairing data of BC1 derivatives indicated that either some of the wheat chromosomes were eliminated or Agropyron chromosomes caused reduced pairing of wheat homologues.
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  • 20
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    Theoretical and applied genetics 66 (1983), S. 153-157 
    ISSN: 1432-2242
    Keywords: Inheritance ; Culm-length ; GA insensitivity ; Grain weight ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The study was carried out in the first year on samples of random F5 lines, uniform in height and in heading date, of three crosses between semi dwarf spring wheat cultivars (Triticum aestivum L.), differing in grain weight and in their Rht gene. In the second year only the progenies of the early heading F5 lines were studied. All the material was grown in the absence of lodging. The culm-length genotypes of the different lines were identified by test crosses and by a seedling GA response test. No differences in grain weight were found between the two semi dwarf genotypes (Rht 1Rht1 rht 2rht2 and rht 1rht1 Rht 2Rht2). The tall genotype (rht 1rht1 rht 2rht2) was significantly higher in grain weight than the two semi dwarf genotyes and the grain weight of these genotypes exceeded markedly the grain weight of the dwarf genotype (Rht 1Rht1 Rht 2Rht2). These genotypic effects were independent of differences in plant height, heading date or number of grains per spike.
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  • 21
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    Theoretical and applied genetics 66 (1983), S. 249-256 
    ISSN: 1432-2242
    Keywords: Wheat ; Dwarfism ; Yield ; Quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of the ‘Tom Thumb’ Rht3 dwarfing gene in wheat on plant height, yield components and grain quality in field trials of random inbred lines from two ‘Minister Dwarf’ (Rht3) x rht3 crosses are described. Limited genetic increases in Rht3 plant height, up to 70 cm, were achieved by incorporating genes from the extremely tall variety ‘April Bearded’. Amongst lines from the higher yielding cross, the dwarfing gene did not reduce grain yield but was associated with an increase in grain number of over 25%. Although Rht3 reduced grain protein content by up to 0.8%, breadmaking quality, as measured by SDS sedimentation values and baking tests, was not affected. A controlled environment experiment with near-isogenic Rht3 and rht3 lines indicated that reduced grain size in the dwarfs is a competitive response to the increase in fertility rather than a primary effect of the dwarfing gene.
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  • 22
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    Theoretical and applied genetics 67 (1983), S. 53-58 
    ISSN: 1432-2242
    Keywords: Aegilops umbellulata ; Genetics ; Lectin ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Each of the three genomes in hexaploid wheat controls the expression of a specific lectin in the embryo. The chromosomes which control their synthesis were determined using nullisomic-tetrasomic and inter-varietal chromosome substitution lines of ‘Chinese Spring’. All three wheat lectins were shown to be controlled by the homoeologous group 1 chromosomes. Using ditelosomic lines of ‘Chinese Spring’ the lectin genes could be localized on the long arms of chromosomes 1A and 1D. Inter-specific addition and substitution lines of Aegilops umbellulata chromosomes to ‘Chinese Spring’ indicated that chromosome 1U, which is homoeologous to the group 1 chromosomes of wheat, controls lectin synthesis.
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  • 23
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    Theoretical and applied genetics 67 (1984), S. 525-528 
    ISSN: 1432-2242
    Keywords: Cryopreservation ; Embryo culture ; Haploids ; Wheat ; Rice ; Germplasm bank
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Anther culture derived pollen embryos and immature zygotic embryos of wheat and rice, frozen in liquid nitrogen in the presence of dimethyl sulfoxide, sucrose and glycerol, have been revived. The retrieved cultures proliferated and/or regenerated shoots and plantlets. The prospects of the cryopreservation of embryos for the conservation and multiplication of germplasm and the possibility of the establishment of ‘Germplasm Banks’ are discussed.
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  • 24
    ISSN: 1432-2242
    Keywords: Wheat ; Triticum ; Grain protein ; Grain weight ; Maternal effect ; Cytoplasmic effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Reciprocal crosses were made between cultivated wheat (Triticum turgidum var. ‘durum’) and a high-protein line of wild tetraploid wheat (T. turgidum var. ‘dicoccoides’). F1 grains (on maternal spikes) were very similar to the selfed grains on the maternal parent in protein percentage, weight and protein content. These traits were also analyzed in F3 grains developed on F2 spikes of segregating populations derived from reciprocal crosses between the same cultivated parent and another high-protein line of var. ‘dicoccoides’. No significant differences in the mean values of these traits were found between the reciprocal crosses, indicating no cytoplasmic effect. It has been concluded that these grain characteristics are largely determined by the maternal plant.
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  • 25
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Gliadins ; Electrophoresis ; Hybridological analysis ; Genetic nomenclature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Inheritance of gliadin components in winter wheat has been studied by one-dimensional polyacrylamide gel electrophoresis. Single F2 grains from 36 intervarietal hybrid combinations have been analysed. The genetic analysis has revealed blocks, including 1–6 gliadin components, which are inherited as individual mendelian traits. About 80 variants of blocks have been detected. On the basis of the allelism test they are grouped into 6 series in accordance with the number of known gliadin-coding loci located on chromosomes of the homoeologous groups 1 and 6. Each series includes 8–18 blocks controlled by different alleles of one gliadin-coding locus. Blocks of components have been confirmed to be inherited codominantly in accordance to the gene dose in the triploid endosperm. The highest similarity between members of one series is observed in groups of blocks controlled by chromosomes ID and 6D. On the contrary, many blocks controlled by chromosomes 1A and 1B have no bands in common. The presented catalogue of blocks of components may be used to make up gliadin genetic formulae and to compare electrophoregrams obtained by different authors. Blocks of gliadin components are suitable genetic markers for use in revealing and studying heterogeneity of wheat varieties, in tracing their origin, in identifying recombinations, translocations and substitutions of the genetic material and in solving many other problems of the origin, evolution and selection of hexaploid wheat.
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  • 26
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    Theoretical and applied genetics 68 (1984), S. 21-23 
    ISSN: 1432-2242
    Keywords: Wheat ; Heritability ; Yield ; Height
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Grain yield, plant height and test weight were studied in a population of winter wheat (Triticum aestivum L.). The population consisted of F2 bulk populations of 42 crosses among 11 genotypes adapted to S. W. Ontario. Heritabilities were: 0.30±0.32 for yield, 0.77±0.15 for height and 0.98±0.08 for test weight. Predicted genetic gain with 10% selection intensity was 0.15 t/ha for yield, 10.1 cm for height and 3.00 kg/hl for test weight. The low heritability for yield indicates that effective selection would require pedigree information and progeny tests, while the high heritabilities for height and test weight indicate that selection for these traits using single plots would be appropriate.
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  • 27
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Gliadins ; Two-dimensional electrophoresis ; Hybridological analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Blocks of gliadin components found both in a number of varieties and in single F2 grains of winter wheat intervarietal hybrids have been studied by two-dimensional electrophoresis combining electrophoresis in acidic aluminium-lactate buffer (pH3.1) and SDS-electrophoresis. Gliadin components (spots) have been shown to be inherited as linked groups (blocks), codominantly and in accordance with a gene dosage in triploid endosperm. Blocks include components differing in their electrophoretic mobility and molecular weight. Some allelic variants of blocks differ only in presence of few additional components or in the electrophoretic mobility of components with similar molecular weights; other variants may contain no similar components. Apparently, in the course of evolution, mutations in individual genes of gliadin-coding loci and processes changing the number of expressing genes and the sizes of their structural part occurred.
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  • 28
    ISSN: 1432-2242
    Keywords: Pollen irradiation ; Wheat ; Differential gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The use of irradiated pollen to bring about limited gene transfer in wheat has been investigated. Doses of X-rays of 2Kr, 3Kr and 5Kr were used to generate M1 progeny between maternal and paternal genotypes differing in quantitative and major gene characters. Cytological studies of M1 plants revealed hybrids with widespread aneuploidy and structural rearrangements in the paternal genome. These effects resulted in phenotypic variation between M1 progeny and complex multivalent formation at meiosis. All M1 plants at the 5Kr and 3Kr doses were sterile and all but 2 plants at the 2Kr dose. Studies of the two M2 families from these plants revealed disturbances in genotype frequencies for some of the marker loci with an excess of maternal homozygotes and a deficit of paternal homozygotes. This was also reflected in a more maternal appearance for quantitative characters. These results are interpreted as showing that irradiation damage to the paternal genome in M1 plants results in the differential transmission of maternal alleles.
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    Theoretical and applied genetics 65 (1983), S. 171-172 
    ISSN: 1432-2242
    Keywords: Resistance ; Genetic engineering ; Yellow rust ; Wheat ; Chitinase
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    Topics: Biology
    Notes: Summary Virulence and resistance may act on the same biochemical mechanisms. Because Erwinia-virulence on potato depends on the lysis of cell walls of the host, resistance may depend on the lysis of cell walls of the parasite. An example is given with yellow rust on wheat.
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    Theoretical and applied genetics 56 (1980), S. 65-69 
    ISSN: 1432-2242
    Keywords: Wheat ; Gliadins ; Chromosomal control ; Aneuploid analysis
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    Topics: Biology
    Notes: Summary The chromosomal location of genes affecting five components from the low molecular weight gliadin (LMWG) fraction from wheat endosperm has been investigated by aneuploid analysis. Genes controlling these proteins were assigned to chromosomes 4B, 7A and 7D. Chromosomes from homoeologous groups 1, 2 and 6, where genes controlling classical gliadins are located, are not involved in the control of LMWG.
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  • 31
    ISSN: 1432-2242
    Keywords: B-Chromosomes ; Chromosome associations ; Triticum aestivum ; Secale cereale hybrids
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    Notes: Summary T. aestivum var. ‘Chinese Spring’ (monosomic 5B and 5D, respectively) was crossed with S. cereale (with and without B-chromosomes). The resulting nullisomic 5B hybrids exhibited a high degree of chromosome association both at 20°C and 10°C. The presence of B-chromosomes reduced association slightly whether 5B was present or not. In nullisomic 5D hybrids B-chromosomes of rye raise chromosome association at 20°C when compared to hybrids with 5D, with as well as without, B's. At 10°C, due to the absence of the Ltp gene on 5D, chromosome association in nullisomic 5D hybrids is low, and no effects of rye B-chromosomes is detectable. The hypothesis that B-chromosomes of rye carry (an) asynaptic gene(s) decreasing effective pairing, and (an) independent post-synaptic gene(s) increasing chiasma frequency on effective pairing sites, is presented.
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    Theoretical and applied genetics 58 (1980), S. 247-252 
    ISSN: 1432-2242
    Keywords: Maternal tissues ; Grain weight ; Wheat ; Triticum aestivum ; Caryopsis ; Pericarp ; Endosperm ; Embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Reciprocal crosses were made between semi dwarf spring wheat cultivars (Triticum aestivum L.) differing in grain weight. The weights of the F1 grains (on maternal spikes), from intact as well as from defoliated plants, and those of the F2 grains (on f1 spikes), were examined. Grain weight was controlled primarily by the genotype of the maternal tissues (pericarp, testa or other floret or spikelet organs, including the vascular system), with complete dominance of heaviness. No indications suggesting maternal inheritance were obtained. The frequency distribution of the weights of the F2 grains indicated the presence of genotypic effects exerted by the endosperm or embryo. The embryo or endosperm factors for heaviness also seemed to be dominant.
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    Theoretical and applied genetics 59 (1981), S. 33-37 
    ISSN: 1432-2242
    Keywords: Wheat ; Rye ; Gene transfer ; Translocations ; Homoeologous genes
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    Topics: Biology
    Notes: Summary By producing chromosome substitutions with ‘Imperial rye’ chromosomes 4R (C) and 7R (D) in the wheat cultivar ‘Chinese Spring’ two spontaneous translocation lines were obtained. One involves segments of wheat chromosome 4A and rye chromosome 7R, the other involves portions of wheat chromosome 7B and rye chromosome 4R
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    Theoretical and applied genetics 59 (1981), S. 145-152 
    ISSN: 1432-2242
    Keywords: Wheat ; Frost resistance ; Diallel cross ; F2monosomics ; Substitutions
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    Topics: Biology
    Notes: Summary Genetic studies of frost resistance were performed on various wheat varieties using diallel, F2 monosomic and substitution analysis. A six-parental cross including reciprocals was carried out, and F1 hybrids and their parents were used for the freezing tests under controlled conditions. Both the general combining ability (GCA) and the specific combining ability (SCA) were significant, indicating additive and non-additive gene action in the inheritance of frost resistance. The high GCA∶SCA ratio revealed a preponderance of additive genetic variance. No significant reciprocal differences were found between the reciprocal crosses. The variance/covariance graphical analysis indicated the partial dominance of frost sensitivity. Frost sensitive varieties had the largest number of dominant genes, while frost resistant varieties had the highest proportion of recessive genes. The magnitude of the additive component of variation was higher than that of the dominance component, and the overall measure of the degree of dominance was smaller than one, so average dominance is incomplete. The increasing and decreasing alleles are not equally frequent at all loci. In this set of wheat varieties the values of narrow and broad heritability are relatively high. F2 monosomic analysis of the winter wheat variety ‘Arthur’ crossed with the monosomics of ‘Chinese Spring’ revealed that the average frost resistance of all the 21 monosomics was lower than that of the disomic. F2 monosomic hybrids 5A, 2B, 4B and 5D proved to be relatively frost resistant, while monosomics 3A, 3B and 6D were the most sensitive. The control of frost resistance in the set of chromosome substitution lines of the variety ‘Cheyenne’ into ‘Chinese Spring’ (with the exception of 2B) indicated that the genes responsible for the frost resistance of ‘Cheyenne’ are localised in chromosomes 5A, 7A, 4B, 5B, 4D and 5D. The genetic basis of frost resistance and problems of analysis are discussed.
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    Theoretical and applied genetics 59 (1981), S. 153-160 
    ISSN: 1432-2242
    Keywords: Mitochondrial heterosis ; Mitochondrial complementation ; Triticum aestivum ; Hordeum vulgare ; Zea mays
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    Topics: Biology
    Notes: Summary Two families each of wheat (Triticum aestivum L.), barley (Hordeum vulgare L.) and maize (Zea mays L.) were studied for mitochondrial heterosis and in vitro mitochondrial complementation. Inbred parents and their hybrids were compared for seedling heights and rate of oxygen uptake by the whole tissue to find out if the hybrids showed greater growth and respiratory activity at the seedling stage. Further comparisons were made by isolating mitochondria from the seedling tissues and measuring their ADP∶0 ratio, respiratory control ratio and cytochrome c oxidase activity for mitochondrial heterosis. Mixtures of parental mitochondria were similarly compared with parental and hybrid mitochondria for in vitro mitochondrial complementation. No evidence for mitochondrial heterosis or in vitro mitochondrial complementation was found, nor any correlation between the different mitochondrial parameters, seedling heights or rates of oxygen uptake by seedling tissue. The suggested use of mitochondrial heterosis and in vitro mitochondrial complementation for plant breeding is discussed.
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  • 36
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Glutenin ; Gliadin ; Electrophoresis
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    Notes: Summary Subunits of wheat endosperm proteins have been fractionated by two-dimensional electrophoresis. To determine which subunits in the two-dimensional electrophoretic pattern belong to gliadin or glutenin the endosperm proteins have also been fractionated by a modified Osborne procedure and by gel filtration on Sephadex G-100 and Sepharose CL-4B prior to separation by two-dimensional electrophoresis. The control of production of five major grain protein subunits is shown to be determined by chromosomes 6A, 6B and 6D by comparing two-dimensional electrophoretic protein subunit patterns of aneuploid lines of the variety ‘Chinese Spring’. From these and previous studies it is concluded that some α, β and γ gliadins (molecular weights by SDS-PAGE 30,000 to 40,000) are specified by genes on the short arms of homoeologous Group 6 chromosomes, the ω gliadins (molecular weights by SDS-PAGE 50,000 to 70,000) are specified by genes on the short arms of homoeologous Group 1 chromosomes and the glutenin subunits (molecular weights by SDS-PAGE 〉 85,000) are specified by genes on the long arms of homoeologous Group 1 chromosomes. No major gliadins or glutenin subunits were absent when any of the chromosomes in homoeologous Groups 2, 3, 4, 5 or 7 were deleted. However two gliadins whose presumed structural genes are on chromosome 6D were absent in aneuploid stocks of ‘Chinese Spring’ carrying two additional doses of chromosome 2A. Two out of thirty-three intervarietal or interspecific chromosome substitution lines examined, involving homoeologous Group 2 chromosomes, lacked the same two gliadins. All the subunits in the other thirty-one chromosome substitution lines were indistinguishable from those in ‘Chinese Spring’. It is therefore concluded that the major variation affecting gliadin and glutenins in wheat is concentrated on the chromosomes of homoeologous Groups 1 and 6 but Group 2 chromosomes are candidates for further study. An endosperm protein controlled by chromosome 4D in ‘Chinese Spring’ is shown to be a high molecular weight globulin.
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  • 37
    ISSN: 1432-2242
    Keywords: Wheat ; Aegilops ventricosa ; Addition lines ; Biochemical markers
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    Notes: Summary The distribution of three biochemical markers, U-1, CM-4 and Aphv-a, -b, among wheat-Aegilops addition lines carrying Mv chromosomes from Aegilops ventricosa (genomes DvMv) has been investigated. Addition lines which had been previously grouped together on the basis of common non-biochemical characters carried marker U-1, a protein component from the 2M urea extract. The added chromosome, in the appropriate genetic background, seems to confer a high level of resistance to the eyespot disease, caused by the fungus Cercosporella herpotrichoides. The other two markers were concomitantly associated with another similarly formed group of addition lines. Both CM-4, a protein component from the chloroform:methanol extract, and Aphv-a, -b, alkaline phosphate isozymes, have been previously shown to be associated with homoeologous chromosome group 4, which suggests that the added chromosome in the second group of addition lines is 4Mv.
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    Theoretical and applied genetics 60 (1981), S. 85-88 
    ISSN: 1432-2242
    Keywords: Wheat ; Leaf rust ; Resistance
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    Notes: Summary The hypothetical leaf rust progress curves of 15 genotypes of wheat were generated by integrating the components of slow rusting resistance determined in the glasshouse. The area under the disease progress curve (AUDPC) from the hypothetical and observed leaf rust progress curves were compared. It was found that the hypothetical AUDPC values of all the genotypes studied were smaller than their respective observed AUDPC values. Possible causes for the discrepancies in the observed and hypothetical values are discussed.
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    Theoretical and applied genetics 60 (1981), S. 161-166 
    ISSN: 1432-2242
    Keywords: Gene expression ; Resistance ; Local virulence ; Stem rust ; Wheat
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    Notes: Summary Near isogenic/substitution lines of stem rust resistance genes in different backgrounds of ‘Marquis’, ‘Chinese Spring’ and W 2691 and certain varieties with known genes for stem rust resistance were tested against each of 19 Indian cultures of stem rust races/biotypes (14, 15, 17, 21, 21A-1, 24, 34, 40, 40A, 42, 42B, 117, 117A, 117A-1, 122, 184, 194, 222 and 295). Sr 24 (‘Sear's 3D/Ag’), Sr 24 (TR 380-27 4/3 Ag 14-White seeded recombinant with ‘Agent’ type resistance), Sr 25 (‘Sear's 7D/Ag’), Sr 26 (‘Eagle’), Sr 26 (Knott's 6A/Ag translocation), Sr 27 (WRT 238-5), Combination line (Sr Tt1 + Sr 9b) were observed to be completely effective against all the 19 cultures tested. In addition, a number of lines, such as TAF2d (Sr Agi), Line W(Sr Tt2) and Combination III (Sr Tt1 + Sr 9e), were found to be effective against at least three of the most prevalent races (21, 40A and 117A-1) and a virulent race 122 in Indian natural population. Lines carrying genes other than Sr 2, Sr 9a, Sr 9f (‘Chinese Spring’) and Sr 15 (‘Norka’), and Line E were found to be resistant to one or more cultures of stem rust. The background effect upon the expression of a gene was observed by comparing the range of infection on single gene host lines in either different backgrounds and/or in cultivars with known genes for stem rust resistance against the 12 cultures of stem rust races found in India.
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    Theoretical and applied genetics 60 (1981), S. 157-160 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Puccinia graminis ; Stem rust resistance ; Inheritance
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    Topics: Biology
    Notes: Summary Two bread wheat accessions, E5008 and E6160, have been genetically analysed for resistance genes effective against Indian cultures of stem rust races, 15C and 122. The inheritance of resistance to each race has been determined from the F1 and F2 of the crosses (resistant parents with the susceptible variety, ‘Agra Local’) and F2 progenies from the backcross to ‘Agra Local’. Tests have been performed to see if the two varieties carry common genes/s for resistance. The identity of the genes for resistance has been established from relevant crosses with single gene lines carrying known genes for resistance. A single dominant gene effective to race 15C in E5008 has been demonstrated to be Sr9b. Of the two recessive genes, each producing distinct infection types (0; and 1–3) against race 122, one gene has been inferred to be Sr12 and the second to be a hitherto undesignated gene. The resistance of E6160 against race 15C is controlled by two genes, one dominant and one recessive. The dominant gene has been identified as Sr9b. The recessive gene has been inferred to be a new gene. Similarly, a dominant gene effective against race 122 in E6160 has been observed to be different from those so far designated. In addition, the presence of modifier gene/s in the variety, E6160 has been suggested.
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  • 41
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    Theoretical and applied genetics 60 (1981), S. 333-337 
    ISSN: 1432-2242
    Keywords: Wheat ; Seed proteins ; Glutenin protein ; subunits
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    Topics: Biology
    Notes: Summary The inheritance of the high-molecular-weight (HMW) glutenin protein subunits in hexaploid wheat has been investigated by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis to examine the segregation of these subunits in 496 test-cross seeds. The parents of the f1 hybrid were chosen so that the test-cross seeds segregated for all the HMW glutenin bands. Two glutenin subunits from one parent, believed to be controlled by genes on chromosome 1D, segregated as alternatives to two glutenin subunits from the other parent, a result that supports the assumption that these subunits are controlled by allelic genes at each of two loci that are very closely linked. Similar results were obtained for glutenin subunits believed to be controlled by chromosome IB, which suggests that these subunits are controlled also by allelic genes at each of two loci that are very closely linked. A single glutenin subunit band, believed to be controlled by chromosome 1A, segregated as an alternative to a single glutenin band from the other parent, except that one seed did not possess either band. It was concluded that these bands are controlled either by allelic genes or by nonallelic genes that are very closely linked.
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    Theoretical and applied genetics 61 (1982), S. 27-33 
    ISSN: 1432-2242
    Keywords: Preferential transmission ; In situ hybridization ; C-banding ; Aegilops sharonensis ; Wheat ; Addition ; Substitution
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    Topics: Biology
    Notes: Summary An attempt to produce a set of addition lines of Aegilops sharonensis to the wheat variety ‘Chinese Spring’ produced only one addition line. This was due to preferential transmission of one chromosome from Ae. sharonensis. This chromosome was studied in detail by established cytological methods of chromosome observation and by the newer techniques of C-banding and in situ hybridization of a cloned DNA sequence. The chromosome was found to be partially homologous to an Ae. sharonensis chromosome of similar behaviour in another wheat addition line. The incomplete homology of the two Ae. sharonensis chromosomes was due to the presence of a translocated segment of a wheat chromosome. — Substitution lines of the Ae. sharonensis chromosome for wheat homoeologous group 4 were produced and the Ae. sharonensis chromosome thereby designated 4 S l .
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    Theoretical and applied genetics 61 (1982), S. 129-133 
    ISSN: 1432-2242
    Keywords: Interchange ; Chromosome arrangements ; Common wheat ; Triticum aestivum
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    Topics: Biology
    Notes: Summary Chromosome arrangements of twenty-eight cultivars of common wheat, Triticum aestivum L., from or introduced into Spain are compared with that of ‘Chinese Spring’ taken as a pattern. All the cultivars analyzed differ from ‘Chinese Spring’ by one or two reciprocal translocations. When 12 out of 28 cultivars were compared it was concluded that a minimum number of thirteen interchanges are present, involving at least ten different chromosomes of the complement. The interest of a reappraisal of the rôle of interchanges in the evolution of Gramineae is pointed out.
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    Theoretical and applied genetics 62 (1982), S. 45-48 
    ISSN: 1432-2242
    Keywords: Wheat ; Rye ; Triticale ; Callus cultures ; Alcohol dehydrogenase ; Isoenzymes
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    Topics: Biology
    Notes: Summary Two NAD-dependent alcohol dehydrogenases ADH-1 and ADH-2, under independent genetic control of genes designated as Adh-1 and Adh-2 located on chromosomes 4A, 4B and 4D, have been reported in aestivum wheat (Hart 1980). Only ADH-1 is expressed in developing seeds, dry seeds, pollen and germinating seedlings. ADH-2 can be induced in seedling roots or shoots under conditions of partial anaerobiosis or by certain chemicals. Expression of ADH-1 and ADH-2 isoenzymes was investigated in undifferentiated calli from aestivum and durum wheats, rye, triticale and also in in vitro regenerated roots and leaves from aestivum cultures. Wheat callus cultures originating from seed, mature and immature embryos, mesocotyl and root, as well as cultures grown on media containing different supplements did not show any variation in the overall expression of ADH-1 or ADH-2, although differences in the band intensities were observed. The callus isoenzyme pattern was similar to that observed in roots under anaerobic conditions. Both ADH-1 and ADH-2 were expressed in in vitro regenerated roots but were absent in regenerated leaves. Expression of ADH-1 and ADH-2 in wheat calli seems to be related to the type of differentiation.
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    Theoretical and applied genetics 62 (1982), S. 121-126 
    ISSN: 1432-2242
    Keywords: Wheat ; Quality ; Dwarfism ; Gibberellin ; α-amylase
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    Topics: Biology
    Notes: Summary The effects of the Tom Thumb dwarfing gene, Rht3, on the quality and quantity of grain α-amylase produced during germination and by induction with exogenous gibberellic acid are described. In a season conducive to high sprouting damage the gene reduced α-amylase levels in the field by 77%. Selection among random Rht3 genotypes showed that other genetic factors can be combined with the dwarfing gene to further increase sprouting damage resistance.
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    Theoretical and applied genetics 63 (1982), S. 71-73 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Pollen ; Anther culture ; Callus ; Regeneration ; Plantlets
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    Topics: Biology
    Notes: Summary Pollen shed between 4–8 d from anthers of Triticum aestivum cultured in liquid medium gave rise to calluses. Tillers were harvested at the mid-to late-unicellular pollen stages and chilled for 8 d at 4–5 °C before the anthers were dissected out. Pollen cultures gave about 6 times as many calluses on a per anther basis as anthers cultured on solid medium. With the most productive of 5 cultivars tested, pollen culture results in roughly one callus for each anther used, though the calluses formed by pollen culture were less productive for the regeneration of shoots than calluses derived from anthers cultured on solid medium. The ratio of green to albino shoots is roughly 1∶ 1 for anther cultures but considerably less for pollen cultures.
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    Theoretical and applied genetics 63 (1982), S. 121-124 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Grainprotein ; Gene action ; Regression ; Diallel cross
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    Notes: Summary Diallel crosses between five divergent vulgare wheat cultivars were made in order to evaluate the mode of inheritance and combining ability of grain proteins. Significant differences in grain protein content were found between cultivars and their hybrids. It was established that the inheritance of seed protein in the F1 generation included both additive and non-additive gene action.
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    Theoretical and applied genetics 62 (1982), S. 317-320 
    ISSN: 1432-2242
    Keywords: Wheat ; Rye ; Phosphogluconate dehydrogenase ; Genetic control
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    Topics: Biology
    Notes: Summary The 6-phosphogluconate dehydrogenase (6-PGD) zymogram phenotypes of wheat, rye and their aneuploid derivatives were determined. Two genes involved in the production of 6-PGD isozymes were located on chromosome arms CRL (4 RL) and FRL (6 RL) of “Imperial” rye. On the basis of differential interactions between wheat and rye chromosomes, evidence was obtained that genes located on chromosomes 6 A, 6 BL and 7 BL control 6-PGD isozyme activities in ‘Chinese Spring’ wheat. The wheat and rye 6-PGD zymogram phenotypes were indicative of homoeologous relationships between rye chromosome 6 RL to wheat chromosomes of group 6, and rye chromosome 4 RL to wheat chromosomes of group 7.
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  • 49
    ISSN: 1432-2242
    Keywords: HMW storage prolamins ; Wheat ; Rye ; Barley ; Characterization and purification
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    Topics: Biology
    Notes: Summary Homologous high molecular weight storage prolamins were purified from grain of wheat, rye and barley using combinations of gel filtration, ion-exchange chromatography and preparative isoelectric focusing. Sodium dodecylsulphate polyacrylamide gel electrophoresis showed that the components were single bands with apparent mol.wts. of above 100,000. Molecular weights determined by sedimentation equilibrium ultracentrifugation were considerably lower; 54,700, 67,600 and 69,600 for the components from barley, rye and wheat respectively. Amino acid analysis showed the presence of 13.6 to 16.5 mol% glycine, 29.6 to 34.0 mol% glutamate + glutamine, 11.4 to 13.7 mol% proline and a total of 4.0 to 5.7 mol% basic amino acids. Automated N-terminal amino acid sequencing of the component from wheat showed the presence of cysteine residues at positions 5 and 10, and this is discussed in relation to the possible role of these proteins in the visco-elastic gluten network.
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    Theoretical and applied genetics 63 (1982), S. 39-47 
    ISSN: 1432-2242
    Keywords: C-banding ; Wheat ; Triticale ; Heterochromatin
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    Topics: Biology
    Notes: Summary The C-banding patterns of wheat chromosomes in 7 hexaploid triticale and 7 wheat genotypes are described and compared. All 14 wheat chromosome pairs were individually identified in the triticales and a tetraploid wheat, and all the B and two A genome chromosome pairs in the hexaploid wheat genotypes. Little variation was found between genotypes in the distribution of C-bands but considerable variation was found in their size, total number and total length.
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    Theoretical and applied genetics 63 (1982), S. 337-348 
    ISSN: 1432-2242
    Keywords: Wheat ; rDNA ; Sequence ; Populations ; Variation ; Spacer region ; Triticum
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    Topics: Biology
    Notes: Summary The wheat rDNA clone pTA250 was examined in detail to provide a restriction enzyme map and the nucleotide sequence of two of the eleven, 130 bp repeating units found within the spacer region. The 130 bp units showed some sequence heterogeneity. The sequence difference between the two 130 bp units analysed (130.6 and 130.8) was at 7 positions and could be detected as a 4 °C shift in Tm when heterologous and homologous hybrids were compared. This corresponded to a 1.2% change in nucleotide sequence per ΔTm of 1 °C. The sensitivity of the Tm analysis using cloned sequences facilitated the analysis of small sequence variations in the spacer region of different Triticum aestivum cultivars and natural populations of T. turgidum ssp. dicoccoides (referred to as T. dicoccoides). In addition spacer length variation was assayed by restriction enzyme digestion and hybridization with spacer sequence probes. Extensive polymorphism was observed for the spacer region in various cultivars of T. aestivum, although within each cultivar the rDNA clusters were homogeneous and could be assigned to particular chromosomes. Within natural populations of T. dicoccoides polymorphism was also observed but, once again, within any one individual the rDNA clusters appeared to be homogeneous. The polymorphism, at the sequence level (assayed by Tm analysis), was not so great as to prevent the use of spacer sequence variation as a probe for evolutionary relationships. The length variation as assayed by restriction enzyme digestion did not appear to be as useful in this regard, since its range of variation was extensive even within populations of a species.
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    Theoretical and applied genetics 63 (1982), S. 349-360 
    ISSN: 1432-2242
    Keywords: Chromosomes ; Nucleotides ; Evolution ; Polyploids ; Triticum ; Heterochromatin ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nature of genome change during polyploid evolution was studied by analysing selected species within the tribe Triticeae. The levels of genome changes examined included structural alterations (translocations, inversions), heterochromatinization, and nucleotide sequence change in the rDNA regions. These analyses provided data for evaluating models of genome evolution in polyploids in the genus Triticum, postulated on the basis of chromosome pairing at metaphase I in interspecies hybrids. The significance of structural chromosome alterations with respect to reduced MI chromosome pairing in interspecific hybrids was assayed by determining the incidence of heterozygosity for translocations and paracentric inversions in the A and B genomes of T. timopheevii ssp. araraticum (referred to as T. araraticum) represented by two lines, 1760 and 2541, and T. aestivum cv. Chinese Spring. Line 1760 differed from Chinese Spring by translocations in chromosomes 1A, 3A, 4A, 6A, 7A, 3B, 4B, 7B and possibly 2B. Line 2541 differed from Chinese Spring by translocations in chromosomes 3A, 6A, 6B and possibly 2B. Line 1760 also differed from Chinese Spring by paracentric inversions in arms 1AL and 4AL whereas line 2541 differed by inversions in 1BL and 4AL (not all chromosomes arms were assayed). The incidence of structural changes in the A and B genomes did not coincide with the more extensive differentiation of the B genomes relative to the A genomes as reflected by chromosome pairing studies. To assay changing degrees of heterochromatinization among species of the genus Triticum, all the diploid and polyploid species were C-banded. No general agreement was observed between the amount of heterochromatin and the ability of the respective chromosomes to pair with chromosomes of the ancestral species. Marked changes in the amount of heterochromatin were found to have occurred during the evolution of some of the polyploids. The analysis of the rDNA region provided evidence for rapid “fixation” of new repeated sequences at two levels, namely, among the 130 bp repeated sequences of the spacer and at the level of the repeated arrays of the 9 kb rDNA units. These occurred both within a given rDNA region and between rDNA regions on nonhomologous chromosomes. The levels of change in the rDNA regions provided good precedent for expecting extensive nucleotide sequence changes associated with differentiation of Triticum genomes and these processes are argued to be the principal cause of genome differentiation as revealed by chromosome pairing studies.
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    Theoretical and applied genetics 64 (1983), S. 97-101 
    ISSN: 1432-2242
    Keywords: Common wheat ; Triticum aestivum ; Electrophoresis ; Endosperm proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Total endosperm protein subunits, extracted from the common wheat cultivar Chinese Spring and from some of its aneuploid lines, were fractionated according to their molecular weight (MW) in an improved high resolution one-dimensional sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). The resolution obtained by this method and, in particular, that of the high molecular weight (HMW) glutenin and gliadin subunits approached that of a previous report in which two-dimensional fractionation system based on charge and MW was used. In the cultivar Chinese Spring, 21 discrete protein bands were resolved and the chromosomes controlling many of them were either reconfirmed, or, in some cases, established. The advantages of this high resolution SDS PAGE technique are discussed.
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  • 54
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    Theoretical and applied genetics 64 (1983), S. 231-238 
    ISSN: 1432-2242
    Keywords: Maize ; Wheat ; Rape ; Restriction fragments ; Two dimensional electrophoresis ; Melting profiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This paper deals with the relationship of chloroplast DNA (ctDNA) and cytoplasmic malesterility (CMS) of maize, wheat and rape. Intramolecular heterogeneity and fragment patterns of DNA digested with restriction endonucleases were compared and analyzed. For this purpose methods of agarose gel electrophoresis and two dimensional gel electrophoresis with denaturating solvent concentration gradients were applied. 1) Heat denaturation of ctDNA from a male sterile line in maize yielded a profile with three melting regions. This base sequence heterogeneity was not observed for the ctDNA of its maintainer. 2) Maize ctDNA was digested with EcoR1 and BamH1 restriction endonucleases, wheat and rape ctDNA with EcoR1 only. No significant differences were observed in the comparison of the fragment patterns from the sterile lines and their maintainers with the exception of the sterile line in rape which lacked one fragment present in its maintainer. 3) Two dimensional electrophoresis using a gradient of denaturing agents revealed several differences in number and relative positions of the separated restriction endonuclease fragments between the sterile lines and their respective maintainers in all three tested crops. This raises the possibility that changes in ctDNA may be involved in CMS.
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  • 55
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    Theoretical and applied genetics 64 (1983), S. 303-307 
    ISSN: 1432-2242
    Keywords: Crossability ; Wheat ; Rye ; Hordeum bulbosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Crosses of wheat (Triticum aestivum L.) Group 5 chromosome substitution lines from three different cultivar backgrounds indicate that there may be multiple alleles for reduced crossability with rye (Secale cereale L.) and H. bulbosum on both 5A and 5B chromosomes. No reduction in seed set was observed from any of the 5D substitution lines. It was found that the Kr1 locus on chromosome 5B had a more pronounced affect on both rye and H. bulbosum crossability than the Kr2 locus on chromosome 5A and that the effects of both loci were cumulative. The Kr alleles affected both rye and H. bulbosum crossability in a linear, or additive, fashion. The Kr 2 allele was shown to have little effect on H. bulbosum crossability compared to a significant effect on rye crossability. Tests with tetrasomic and nulli-tetrasomic lines of ‘Chinese Spring’ indicated that the kr allele is ‘null’ or inactive in promoting crossability while the Kr allele is active in reducing crossability to both rye and H. bulbosum. Thus, extra doses of the kr allele do not increase rye or H. bulbosum crossability in the presence of the corresponding Kr allele.
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  • 56
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    Theoretical and applied genetics 64 (1983), S. 309-316 
    ISSN: 1432-2242
    Keywords: Wheat ; α-Amylase ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An analysis of the α-amylase isozymes in GA-treated endosperm of wheat nullisomic-tetrasomics shows that there is more variation at the α-Amy-1 and α-Amy-2 homoeoallelic loci than was previously thought. Among the 16 isozymes produced by genes on the group 7 chromosomes, most could be definitely established as products of a single homoeoallele. Inter-varietal allelic differences would be expected at such loci and clear variation was found in isozymes produced by chromosomes 6B and 7B. The latter allele, α-Amy-B2b carried by the variety ‘Hope’, was used to locate the enzyme structural gene within chromosome 7B relative to the centromere and five other gene markers. The nature of the α-Amy-B2b phenotype and the rare non-parental isozyme patterns found among the recombinant lines indicates that the locus is large and compound, probably involving some degree of intra-locus gene duplication.
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  • 57
    ISSN: 1432-2242
    Keywords: Gliadin ; Glutenin ; Tticum ; Wheat ; Endosperm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Gliadins, here defined as those proteins of defatted wheat endosperm which dissolve in 70% (v/v) ethanol at room temperature, were fractionated by gel filtration using Sephadex G-100. The protein which eluted with the void volume of the column, often described as high-molecular-weight (HMW) gliadin, was fractionated by the two different, two dimensional gel electrophoresis procedures of O'Farrell (1975) and O'Farrell et al. (1977). The next two fractions to elute from the gel column, ω-gliadin and α-, β-, γ-gliadin, were analysed similarly. The subunits of HMW gliadin and the classical (i.e. non-aggregated) gliadins map at distinctive positions on the electrophoregrams, the majority of the HMW gliadin subunits being more basic and having a slightly slower electrophoretic mobility than the α-, β-, γ-gliadins. These experiments demonstrate that those gliadins which aggregate to form HMW gliadin are distinct molecular entities and thus coded by different genes to those gliadins which do not aggregate. Glutenin, here prepared by a modification of the pH 6.4 precipitation procedure of Orth and Bushuk (1973), was also analysed by two-dimensional electrophoresis. The low-molecular-weight subunits were found to correspond exactly with the HMW gliadin subunits. Using the nullisomic-tetrasomic lines and the ditelocentric lines of ‘Chinese Spring’, the genes controlling the synthesis of all the major HMW gliadin subunits were shown to be located on the short arms of chromosomes 1A, 1B and 1D, as are the genes coding for the ω-gliadins and the majority of the γ-gliadins.
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  • 58
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; T. timopheevii ; Wheat ; Photoperiod ; Vernalization ; Male sterility ; Alloplasmic hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Studies were conducted to determine the influence of the male sterility-inducing cytoplasm of Triticum timopheevii (Zhuk.) Zhuk. on response of several common winter wheat (T. aestivum L.) nuclear genotypes to photoperiod and vernalization. Comparative studies of cytoplasmic substitution lines provide information on the role of the cytoplasmic genetic mechanism in growth and development. In the case of cytoplasmic male sterility-based hybrid production systems, ubiquity of sterility-inducing cytoplasm in derived hybrids warrants thorough characterization of its influence on plant phenotype. Factorial combinations of cytoplasm (T. timopheevii and T. aestivum), nuclear genotype, and photoperiod or vernalization treatments were evaluated under hydroponic conditions in controlled environment chambers. Interaction of cytoplasm, photoperiod, and nuclear genotype was significant in one or more experiments for days to anthesis and potential spikelet number, and interaction of cytoplasm, vernalization, and nuclear genotype was significant for days to spike emergence. Long day length was associated with increased percentage seed set in one study, but interactions of photoperiod and cytoplasm were not detected for percentage seed set. Interactions involving cytoplasm and photoperiod or vernalization were interpreted as evidence of the existence of genetic factors in cytoplsam of T. timopheevii which alter photoperiod or vernalization responses of alloplasmic plants relative to responses exhibited by euplasmic plants. Since photoperiod and vernalization responses are critical to adaptation, T. timopheevii cytoplasm can alter adaptability of T. aestivum. The specific effect would be nuclear genotype dependent, and does not appear to be of a magnitude greater than that induced by nuclear genetic variability at loci conditioning photoperiod or vernalization responses or other adaptation-determining characteristics. Normal multilocation/year testing of alloplasmic hybrids should therefore adequately identify zones of adaptation.
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    Theoretical and applied genetics 66 (1983), S. 77-86 
    ISSN: 1432-2242
    Keywords: Common wheat ; Triticum aestivum ; Electrophoresis ; Endosperm proteins ; Glutenins ; Gliadins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Endosperm protein subunits of 109 primitive and modern lines of hexaploid wheat, Triticum aestivum L. em. Thell., were fractionated by one-dimensional, high resolution, sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). A wide range of both qualitative and quantitative variation was observed in the fractions of the high molecular weight (HMW) glutenin and gliadin subunits of the different lines. The qualitative variation was expressed in the number of subunits per fraction and in their molecular weight, as determined by the differential rate of migration. The quantitative variation was expressed in the differential staining intensity of several subunits. The widest variation was detected in the HMW glutenin and gliadin subunits controlled by chromosome 1B while a much smaller variation was observed in those subunits controlled by chromosome 1A and further smaller variation in the subunits controlled by 1D. Only a small number of subunits in both fractions was found to be controlled by chromosome 1A indicating that diploidization of endosperm protein genes in common wheat has been non-random. The genetic and evolutionary implications of these findings are discussed.
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    Plant and soil 80 (1984), S. 135-138 
    ISSN: 1573-5036
    Keywords: Hardpans ; Root growth ; Soil compaction ; Subterranean clover ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat crops with stunted chlorotic patches are widespread in northern Victoria, Australia, and are often associated with dense, compacted layers of soil. Poor growth of subterranean clover, with symptoms of cupped and reddened leaflets, is also a problem in these cropping regions during the pasture phase of the rotation. Artificially compacted soils were created to test the hypothesis that these symptoms of poor growth were caused by soil compaction. Soil compacted from 0–20 cm with a bulk density similar to that measured in problem fields reproduced these symptoms in wheat and subterranean clover. Surface compaction alone also reproduced the symptoms in clover.
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  • 61
    ISSN: 1573-5036
    Keywords: Azospirillum lipoferum ; Grain yield ; N-content ; Nitrogen fixation ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Spring wheat (Triticum aestivum var. Arkas) was associated withAzospirillum lipoferum under greenhouse and field conditions of a temperate region. Controls were treated with autoclaved bacteria. The soils used were: sand, sandy loam, and a peat-clay mixture. In experiments run over a period of three years, there were increases in grain yield, N-yield of the grains, and 1000 grain weight. Depending from environmental conditions, increase changed from year to year, and within one given year. There was, however, no experiment without positive response to the inoculation. Highest grain yield increase (70%) was found on sand supplemented with P and K only, but up to 32% were also obtained on peat-clay soil containing 0.28% total N. Under greenhouse conditions, one third of technical N-fertilizer could be saved by bacterial activities. With high probability the effects observed have been at least partly due to bacterial N2-fixation, because the N-yield of the grains was increased (up to 33%), and the most pronounced response was found on sand without any N-fertilizer added.
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  • 62
    ISSN: 1573-5036
    Keywords: Lignin ; Manganese ; NO 3 − Phenols ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Managanese deficiency (〈 18 μg g−1 Mn) resulted in decreased levels of phenols in wheat shoots and decreased levels of lignins in both roots and shoots. These observed reductions in phenol contents was due largely to a decrease in the alkaline labile phenol component. Levels of nitrate supplied in solution influenced both phenol and lignin production; high nitrate levels (15 mM) resulted in a reduction in phenol and lignin in the shoot but stimulated lignin production in root tissue.
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  • 63
    ISSN: 1573-5036
    Keywords: Azospirillum brasilense ; Wheat ; Triticum aestivum ; N-fertilizer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The yield response of a common spring wheat cultivar,Triticum aestivum, to inoculation withAzospirillum brasilense was studied at four levels of N fertilization. Plant yield increased due to the inoculation treatment only at medium and high levels of N fertilization, with a maximum yield increase of about 8.0 per cent at the highest level (approximately 1.0 g of pure N per plant). Yield increase was mostly due to an increase in the number of grains per spike, and at the highest level of fertilization, also due to a higher number of spikes per plant. At all N levels, the inoculation caused an increase of 0.5–1.4 per cent in the number of fertile spikelets per main spike. Grain protein percentage was unaffected by the inoculation, though significantly increase due to the fertilization treatments. The occurrence of maximum yield response at the highest N level, the response by early-determined yield components, i.e. spikelet number, and the unaffected grain protein content are in accord with the suggestion that the contribution ofAzospirillum brasilense to wheat yield is not through N2-fixation.
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  • 64
    ISSN: 1573-4927
    Keywords: nitrate reductase ; Triticum aestivum ; ditelosomics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The nitrate reductase activities (NRA) of 31 ditelosomic stocks were compared with that of the control plant [Chinese Spring (CS) euploid], using in vivo and in vitro assay procedures that had been optimized with respect to the euploid. Fourteen stocks exhibited significant differences in in vivo NRA from that of the euploid; the effect of removal of a chromosome arm was always to increase NRA. Eight of these stocks showed similar effects in vitro, although in three, a casein-sensitive factor had to be eliminated before the difference was expressed. Homoeologous group effects were evident among ditelosomics of groups 2, 4, and 7, while for three chromosomes (2D, 7A, and 7B), removal of either arm resulted in a similar increase in NRA in vivo and probably in vitro.
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    European journal of plant pathology 89 (1983), S. 87-98 
    ISSN: 1573-8469
    Keywords: Wheat ; Septoria ; epidemiology ; crop loss
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting In 1980 werden inoculaties verricht metSeptoria tritici in een veldproef met wintertarwe ‘Okapi’. De bladnecrose-voortschrijdingscurves werden bepaald. De S-vormige curves konden getransformeerd worden tot logit-lijnen. De geïnoculeerde en niet geïnoculeerde objecten verschilden aanzienlijk in de hellingen van de logit-lijnen. De halfwaardetijden varieerden al naar inoculatiedosis en vochtbehandeling na inoculatie. De verschillen in opbrengst (kg ha−1) waren significant voor geïnoculeerd tegenover niet-geïnoculeerd. De opbrengstderving van 878 kg ha−1 (gelijk aan 12% van de niet-geïnoculeerde controle 7045 kg ha−1)werd volledig verklaard door de vermindering van het korrelgewicht. De schade is aanzienlijk voor een cultivar die niet bijzonder vatbaar is. De opbrengstderving correleerde goed met de hoeveelheid necrose waargenomen bij de ontwikkelingsstadia DC=75 en DC=77. De necrose geïntegreerd over de tijd correleerde goed met de verlaging van het korrelgewicht.
    Notes: Abstract In 1980, field inoculations withSeptoria tritici were performed on winter wheat cv. Okapi. Leaf necrosis progress curves were established; they were sigmoid and could be transformed into logit lines. There was a great difference in the slope of the logit lines between inoculated plots and non-inoculated controls. The mid-time values varied according to inoculum dosage and post-inoculation humidity treatment. Yields (kg ha−1) and average grain weights (mg grain−1) differed significantly between inoculated and non-inoculated plots, but seldom among inoculation treatments. Decrease of average grain weight completely explained yield loss, which was 878 kg ha−1 or 12% of control (7045 kg ha−1). This crop loss is considerable for a cultivar which is not particularly susceptible. A good correlation was found between the amount of necrosis at development stages DC=75 and 77 and yield depression. The integral of necrosis over time gave a high correlation with loss of kernel weight.
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    Nutrient cycling in agroecosystems 5 (1984), S. 285-288 
    ISSN: 1573-0867
    Keywords: Wheat ; potassium chloride ; irrigation ; plant water potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract ‘The water potential of wheat plants increased during the mid-day noon, probably as an adaptation; the rise was greater with less frequent irrigation and was increased by KCl application.’
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    Plant and soil 54 (1980), S. 77-94 
    ISSN: 1573-5036
    Keywords: Nutrients ; Oxygen ; Roots ; Soil ; Toxins ; Waterlogging ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of waterlogging on concentrations of gases and various solutes dissolved in the soil water were investigated in the laboratory, to determine whether the early disruption to the growth of wheat was most closely associated with depletion of dissolved oxygen, accumulation of toxins, or changes in concentrations of nutrient ions in the soil water. Waterlogging slowed shoot fresh weight accumulation, leaf extension and nodal root growth; it also caused death of the seminal root system and early senescence of the lower leaves. However, the shoot dry weight initially increased above that of the non-waterlogged controls, and thus was not a reliable indicator of the early restriction to plant growth and development. The symptoms of damage to shoots and roots were attributed to the fall in soil oxygen concentrations, rather than to any decrease in concentration of inorganic nutrients in the soil water, or to the accumulation of any other measured solutes to toxic concentrations.
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    Plant and soil 56 (1980), S. 187-199 
    ISSN: 1573-5036
    Keywords: Aeration ; Anaerobic ; Flooding ; Inorganic nutrients ; Mineral nutrition ; Nutrient accumulation ; Triticum aestivum ; Urea ; Waterlogging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Decreases in the concentrations of nitrogen, phosphorus, potassium, calcium and magnesium, in the shoots of wheat seedlings soon after the start of waterlogging were mainly attributed to an inhibition of ion uptake and transport by roots in the oxygen deficient soil. There was a small net accumulation of nitrogen, phosphorus and potassium by the aerial tissues, principally the tillers rather than the main shoot. By contrast, calcium and magnesium accumulated in both tillers and main shoot. With waterlogging, nitrogen, phosphorus and potassium were translocated from the older leaves to the younger growing leaves, and in the case of nitrogen this was associated with the onset of premature senescence. Calcium and magnesium were not translocated from the older leaves, the younger leaves acquiring these cations from the waterlogged soil. The promotion of leaf senescence by waterlogging was counteracted by applications of nitrate or ammonium to the soil surface, or by spraying the shoots with solutions of urea, but the beneficial effects on shoot growth were small. The role of mineral nutrition in relation to waterlogging damage to young cereal plants is discussed.
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    Plant and soil 57 (1980), S. 167-175 
    ISSN: 1573-5036
    Keywords: Accumulation ; Barley ; Cations ; Electrical conductivity ; K/Na ratio ; Proline ; Saline water ; Stress ; Tolerance ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a micro-plot experiment it was observed that free proline accumulation in barley and wheat crops increased with the salt stress. But in sensitive strains of both the crops it did not accumulate in accordance with the preceding values after their tolerance limit and at this point yield of barley was reduced by 25.8% and wheat by 23.2% over control while significant yield reductions were not observed with tolerant strains. Proline accumulation was also found to be related to tissue K/Na ratio and the critical limits of this ratio were observed between 0.60 and 0.34 for barley and 4.2 and 1.4 for wheat.
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    Plant and soil 57 (1980), S. 467-470 
    ISSN: 1573-5036
    Keywords: Aluminum concentration ; Aluminum toxicity ; Calcium ; Magnesium ; Nutrient solution ; Phosphorus ; Potassium ; Triticale ; Rye ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of A1 on the growth and mineral composition of different cultivars of triticale (X Triticosecale, Wittmack), wheat (Triticum aestivum L.) and rye (Secale cereale L.) growing in 1/5 strength Steinberg solutions containing 0 or 6 ppm A1 were evaluated after 32 days. Aluminum increased the concentrations of P and K in the roots and K in the tops of most of the cultivars tested. A1 tolerant triticale retained a lower concentration of Mg in the roots and tops than the A1 sensitive triticale, when subjected to A1 stress. In addition, A1 treatments resulted in smaller increases in root P for the A1 tolerant triticale than for the A1 sensitive cultivars. The concentration of root Ca and P of the A1 tolerant wheat cultivars were significantly below that of the more sensitive plants. Aluminum tolerance in rye appeared to be associated with lower Ca and higher Mg concentrations in the tops. The accumulation of P and A1 in the roots was characteristic of sensitivity in triticale, wheat and rye.
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    Plant and soil 59 (1981), S. 119-125 
    ISSN: 1573-5036
    Keywords: Intracoleoptile internode ; Water uptake ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Measurements of xylem vessel number and radii in the seminal roots and intra-coleoptile internode (I.C.I.) of five wheat genotypes showed that the conducting capacity of the I.C.I. was close to the main seminal axis, and would restrict flow when the usual 3–5 seminal axes contributed to uptake. The length and hence resistance of the I.C.I. increased with sowing depth, whilst xylem diameter also fell in two genotypes, which would further restrict flow. The resistance per unit length of I.C.I., assuming Poiseuille flow, was 4×10−4 cm−4 day MPa. A pressure drop of 0.15 MPa along an I.C.I. 5 cm long would be required to maintain transpiration under typical field conditions in southern Australia in spring. In a second study of eleven wheat varieties sown up to 10 cm deep, maximum I.C.I. length ranged from 3.6–6.8 cm amongst varieties with similar maximum coleoptile lengths (6–8 cm). Thus considerable variation in hydraulic resistance may be achieved by the appropriate combination of genotype and planting depth. It was concluded that potentially useful differences in the rate of subsoil water use could result.
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  • 72
    ISSN: 1573-5036
    Keywords: Amino acids ; Proteins ; Wheat ; Salinity ; Soil types
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of four lysimeter soil series under three salinity levels were evaluated for grain yield, wt/1000 seeds, protein, and amino acids in Mexican dwarf wheat (Triticum aestivum L. var. Cajeme 71). The soil series consisted of: Holtville clay loam, Greenfield sandy loam, San Emigdio sandy loam, and Altamont clay loam. The irrigation water salinity levels were designated: low −2.2 mmho, medium −4.2 mmho, and high −7.1 mmho. No significant differences were found in the amount of grain harvested or wt/1000 seeds in the 1976 crop produced on the differential soil series. The yield of the 1977 crop was significantly affected by the soil types. Effects of soil type on the protein amino acids in the grain in both years were similar. Significantly higher protein amino acid levels of histidine, arginine, aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, and phenylalanine were found in the grain grown on Altamont clay loam soil than the other types. The free amino acids in grain from the 1976 and 1977 crops were similarly affected by the soil types, except that the quantitative values of the free amino acids were substantially lower in 1977 than in 1976. The free amino acids significantly influenced by soil types were tryptophane, lysine, arginine, aspartic acid, threonine, serine, glycine, alanine, valine, isoleucine, tyrosine, and phenylalanine. In both years' crops, the sum of the free amino acid fractions was significantly higher in the grain produced on the Altamont soil than on the other soils. Salinity level in the irrigation water did not affect the 1976 crop yield or wt/1000 seeds. Although yields of the 1977 crop were significantly reduced by salinity, the wt/1000 seeds was not. The sum of protein amino acids was significantly higher in the 1976 and 1977 grain crops irrigated with high salinity water than in low salinity irrigated crops. An increased salinity irrigation water significantly reduced the sum of free amino acid fractions in the 1976 grain crop. Since some of the free amino acids in the 1977 grain crop increased while the others decreased due to the salinity level in the irrigation water, the sum of the free amino acid fractions was not significantly influenced. Significant interactions were found between soil types and salinity levels on free arginine, threonine, serine, glutamic acid, and alanine, and also on the sum of the free amino acids in the 1976 wheat grain. In the 1977 wheat grain, there were significant interactions between soil types and salinity levels on the free glutamic acid, valine, leucine, tyrosine, and phenylalanine, and on protein serine, glutamic acid, glycine, alanine, and the sum of the protein amino acids. The amounts of essential amino acids expressed as mg of amino acid/g of protein were not affected by the soil types or salinity levels. With the exception of lysine, and possibly threonine and methionine plus cystine, the essential amino acids were present in the grain at concentrations equal to or greater than recommended by WHO and FAO.
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  • 73
    ISSN: 1573-5036
    Keywords: Acridine orange ; Barley ; Cerelas ; Microdochium bolleyi ; Nuclear staining ; Phialophora radicicola ; Rhizosphere ; Root cortex death ; Take-all ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nuclear staining with acridine orange was used to assess cell viability in the cortex of wheat and barley seminal roots from glasshouse and field experiments. Results from this method correlated well with nuclear assessments made in unstained or Feulgen-stained roots, and other evidence is presented to support the validity of the method. The pattern of root cortex death (RCD) was similar in wheat and barley and consistent over a wide range of conditions. Behind the extending root tip and zone of nucleate root hairs, nuclei disappeared progressively from the outer five (of six) cortical cell layers of the root axes, starting in the epidermis. Stainable nuclei remained in the sixth cell layer, next to the endodermis, and in most cell layers around the bases of root laterals and in a small region immediately below the grain. The onset of cell death was apparently related more to the age of a root region than to its distance behind the root tip, and it was not closely correlated with endodermal or stelar development assessed by staining with phloroglucinol/HCl. The rate of RCD was much faster in wheat than barley in both glasshouse and field conditions, and faster in some spring wheat cultivars than in others in the glasshouse. RCD occurred in sterile vermiculite and perlite and was not enhanced by the presence of soil microorganisms; nor was it enhanced in soil by the addition of the non-pathogenic fungal parasitesPhialophora radicicola var..graminicola orMicrodochium bolleyi. RCD is suggested to be endogenously controlled by the amount of photosynthate reaching the cortex. Its implications for growth of soil microorganisms and especially for growth and biological control of root-infecting fungi are discussed.
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  • 74
    ISSN: 1573-5036
    Keywords: Mg/Ca ratio ; Water quality ; Nutrient content ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A study conducted in pots to evaluate the effect of different Mg/Ca ratios (2, 4, 8 and 16) and electrolyte concentrations (20 and 80 meq/l) at SAR 10 in irrigation water on the nutrient uptake and yield of wheat crop in two soils revealed that the average grain and dry matter yields of wheat decreased significantly with an increase in Mg/Ca ratio in irrigation water, but the magnitude of decrease was greater at higher electrolyte concentration than at lower electrolyte concentration. The concentration of Na in both straw and grain of wheat increased and that of K decreased with an increase in Mg/Ca ratio and electrolyte concentration of irrigation water, which led to higher Na/Ca and Na/K ratios in the plant. Further, the concentration of Ca and Mg both in straw as well as in grain increased with increasing electrolyte concentration of the irrigation water. An increasing proportion of Mg in saline irrigation water resulted in decreased concentration of Ca and increased concentration of Mg in both straw and grain of wheat crop. It was also noticed that the increasing proportion of Mg over Ca in the poor quality irrigation water increased the P content of both straw and grain of wheat crop.
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  • 75
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    Plant and soil 65 (1982), S. 375-382 
    ISSN: 1573-5036
    Keywords: Activity ratio ; Free energy ; Pearl millet ; Potassium supplying capacity ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soil samples from a long term field experiment, to study the K depletion pattern, were analysed for K. The values of supply parameters pK−1/2p (Ca+Mg) and free energy δG r 0 were calculated using the data on water soluble and exchangeable cations. Correlation coefficient values between pK−1/2p (Ca+Mg) vs. total K removed, K removal by pearl millet in 1980 and soil available potassium were positive and statistically significant. This indicated that these soils have reached the stage where they would start responding to potassium application. Also, negative but significant correlation of δG r 0 with pK−1/2p (Ca+Mg) and available K, were found. δG r 0 values were positive indicating the affinity of such soils for K and the values, for K fertilized plots were lower in comparison to plots where no K was applied. pK−1/2p (Ca+Mg) parameter was found to be a better measure of K availability over δG r 0 due to either fixation of K or greater contribution of nonexchangeable K fraction towards plant utilized K.
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  • 76
    ISSN: 1573-5036
    Keywords: Acridine orange ; Cochliobolus sativus ; Common root rot ; Nitrogen fixation ; Nuclear staining ; Roots ; Senescence ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Natural senescence of the root cortex was assessed by nuclear staining, for cultivars and chromosome substitution lines of spring wheat known to differ in (1) susceptibility to common root rot, (2) total rhizosphere populations and (3) ability to support growth of a free-living nitrogen-fixing bacterium. Together, three root rot susceptible wheat lines showed significantly more cortical senescence than did three resistant lines; the susceptible lines also support larger rhizosphere populations. The wheat line that supports growth of a nitrogen-fixing bacterium showed significantly less cortical death than did any other line. Substitution of chromosome pairs 5B or 5D between the parent cultivars Rescue and Cadet substantially altered the amount of root cortex death, which is thus genetically determined. It is suggested thatCochliobolus sativus and other weak parasites benefit from early natural senescence of the root cortex, and that the degree of susceptibility or resistance of wheat lines to common root rot is at least partly determined by differences in cortical senescence.
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  • 77
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    Plant and soil 66 (1982), S. 433-437 
    ISSN: 1573-5036
    Keywords: Kaolin spray ; Mulch ; N-application ; Split application ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Studies revealed that the application of fertilizer nitrogen brought a significant increase in grain and straw yield of wheat. The significant effect was also noticed on such yield contributing characters like number of effective tillers per metre row length, spike length, and number of grains per spike. The increase of nitrogen level from 40 kg/ha to 80 kg/ha also brought a significant increase in yield and yield contributing characters. The application of entire dose of nitrogen at the time of sowing was as good as its split application. The application of nitrogen also influenced the nitrogen concentration of grains whereas, the other treatments did not influence the nitrogen concentration in grains or straw. The nitrogen treatments did not influence the moisture content of soil. The application of mulch or mulch+Kaolin resulted in significantly higher content of soil moisture in 0–15 cm soil depth as compared to control or Kaolin spray alone which was simultaneously reflected in yield and yield contributing characters.
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  • 78
    ISSN: 1573-5036
    Keywords: Calcareous soil ; Chloride ; Dry matter ; Nitrogen ; Phosphate ; Soil salinity ; Triticale ; Triticum aestivum L. ; Water stress ; Wheat ; X Triticosecale Wittmack
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three wheats and one triticale were grown, up to flowering stage, in pots on calcareous soil adjusted to a range of salinities (S1=3.5, S2=6, S3=8.5, and S4=11 mmhos/cm, 20°C, soilpaste extract) by adding solution consisting of 3∶2∶1 of Na-, Ca- and Mg chlorides in chemical equivalent amounts. Moisture in the pots was kept at 100% (W1), 40% (W2) and 20% (W3) of the available water. The vegetative growth, nitrogen and phosphate were affected by S and W treatments, chloride was affected only by S. The interaction S×W affected only dry weight. Varietal effect was observed between wheat as a group and triticale. Multiple quadratic regression equations of these properties on salinity and water revealed that the higher the available water the wider the range of tolerable salinity. Triticale was relatively more tolerant to water stress. Salinity increases Cl and decreases N, whereas water stress enhances N accumulation to a certain extent. However, in triticale at S3 and S4 the effect of water stress on N was overshadowed by the excessive salinity. This did not occur for the wheat (Florence). P trends were described. R2 for P was low (0.7435–0.3603) which made interpretations rather difficult.
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  • 79
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    Plant and soil 68 (1982), S. 139-141 
    ISSN: 1573-5036
    Keywords: Humic acids ; Silica ; Triticum aestivum L. ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The weathering of soil minerals by humic acids is linked with increased silica content of wheat plants.
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  • 80
    ISSN: 1573-5036
    Keywords: Aeration ; Calcium ; Carbon dioxide ; Ethylene ; Nitrate ; Nitrite ; Nitrous oxide ; Oxygen ; Potassium ; Roots ; Triticum aestivum ; Waterlogging ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We report a study of the mechanism by which the response of plants to waterlogging can be modified by soil temperature. Wheat was grown initially in well-aerated soil in a controlled environment room before the soil was flooded with aerated, deionized water. The soil temperature was maintained constant in the range 6–18°C while the air temperature was at 14°C. Waterlogging damage was greater in plants at the higher soil temperatures when the plants were compared at the same chronological age. However, when compared at the same growth stage, the response to soil temperature was little differenti.e. plants subjected to waterlogging for a long time at low soil temperatures exhibited a similar reduction in growth and other properties as those subjected briefly at higher temperatures. The concentration of dissolved oxygen in the soil solution declined rapidly at all temperatures, being almost zero after 36 h waterlogging. Temperature affected rates of change of the concentrations of dissolved carbon dioxide, ethylene, nitrous oxide, nitrite, nitrate, calcium and potassium. The importance of soil-and plant-determined properties in the waterlogging response of plants at different temperatures are discussed.
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  • 81
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    Plant and soil 75 (1983), S. 51-61 
    ISSN: 1573-5036
    Keywords: Aggregate fractions ; Fatty acids ; Long-term rotation ; Triticum aestivum ; Water-stable aggregates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three non-replicated, unfertilized, dryland grain rotations—continuous wheat, wheat-fallow, and wheat-wheat-fallow—were established in 1912 on a Dark Brown Chernozemic (Typic Haploboroll) soil. The effect of long-term cropping on the chemical constituents of total water-stable aggregates was assessed. There was a loss in percentage of total water-stable aggregates and a shift in aggregate size distribution with time. Together with an increase in the 100 μm diameter fraction, there was an increase in the sand component of this fraction. These sand particles are probably held together by alkaline-soluble, acid-insoluble organic matter. Organic carbon, polysaccharides, polyuronides, phenols, and chloroform/methanol-extractable organic matter were all associated with the 〉250 μm diameter fractions. Although the aggregates had generally the same suite of aliphatic carboxylic acids, the relative proportions changed with cultivation.
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  • 82
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    Plant and soil 78 (1984), S. 417-428 
    ISSN: 1573-5036
    Keywords: Aphelenchoides spp ; Aphelenchus avenae ; Pinus radiata ; Soil fungi ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The soils of a wheat-field and pine-forest had different mycofloras and supported different populations ofAphelenchus avenae, a mycophagous nematode. The abundance ofA. avenae was correlated with the composition of the mycoflora in these soils; the greater abundance ofA. avenae in wheat-field soil being associated with a more diverse mycoflora.
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  • 83
    ISSN: 1573-5036
    Keywords: Ammonium acetate extractable potassium ; Inceptisol soil ; Minimum level ; Non-exchangeable potassium ; Pearl millet ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ammonium acetate extractable potassium in the soil reached a minimum value of 6.8 mg K/100g soil after 14 crops of wheat and pearl millet in the field without applying any potassium fertilizer. At this level of ammonium acetate extractable K both wheat and pearl millet utilized about, 90 per cent of the total K from non-exchangeable sources. Wheat and pearl millet were grown in this soil in the greenhouse at different levels of K. At K0 level wheat utilized 86 per cent of the total K uptake from the non-exchangeable source and pearl millet, 95 per cent. At K1 level, wheat utilized only 19 per cent but at higher levels of K, there was build up in the K status of soils. In the case of pearl millet at K1, K2 and K3 levels 59, 13 and 22 per cent of total uptake were contributed by non-exchangeable forms. The total K uptake by pearl millet was more than double that by wheat. Plant analysis showed that 83 per cent of the total K in wheat was contained in the shoot portion and the rest in the roots. The corresponding figures for pearl millet were 94 and 6 per cent.
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  • 84
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    Plant and soil 78 (1984), S. 441-444 
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis vartritici ; Hay-die ; Manganese deficiency ; Take-all ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The hypothesis that wheat plants deficient in managenese are predisposed to infection byGaeumannomyces graminis is outlined, and a test of the hypothesis in a soil system is reported. The results supported the hypothesis: wheat plants growing in managanese-deficient soil, although not showing foliar symptoms, were markedly more susceptible to infection; plant analysis confirmed the nutrient status of the plants. A review of the literature on take-all in wheat coupled with the results of our experiments suggests a reinterpretation of the etiology of this disease, since those edaphic factors which promote infection by this organism are those which also render managese unavailable to the host. Managenese nutrition is proposed as a common factor in many of the environmental conditions which influence the host-pathogen balance.
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  • 85
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    Plant and soil 79 (1984), S. 11-28 
    ISSN: 1573-5036
    Keywords: Beta distribution ; Buckling ; Cracks ; Crack width ; Lateral roots ; Pea ; Penetration ; Penetrometer ; Rape ; Remoulded soil ; Roots ; Safflower ; Seminal axes ; Soil strength ; Undisturbed soil ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary It is shown that probabilities of root penetration across cracks in soil can be calculated effectively using a mathematical model involving root stress and soil distributions and penetrometer/root stress ratios. Penetration criteria are developed, and it is found that the effective penetrometer/root stress ratios take values of about 4 for crack widths smaller than about 2 mm and about 8 for wider cracks. Root swelling does not appear to contribute significantly to the probability of root penetration through any effect on root buckling stress. Suggestions are made for further work on the effects of soil structure and strength on root behaviour.
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  • 86
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    Plant and soil 80 (1984), S. 261-266 
    ISSN: 1573-5036
    Keywords: Azospirillum brasillense ; Wheat yield ; Triticum aestivum ; Responding genotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twenty different wheat genotypes representing a wide genetic variability, were tested for their yield and yield components response to inoculation withAzospirillum brasilense, at two levels of N fertilization. Only two cultivars responded by a significant yield increase of 7.4 and 8.0 per cent — both at the higher N level. The response reflected an increase in the number of grains per plant added as additional spikes. The importance of the host plant genotype for a successful wheat-bacteria association is discussed.
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  • 87
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    Euphytica 29 (1980), S. 727-736 
    ISSN: 1573-5060
    Keywords: Wheat ; Triticum aestivum ; bread wheat ; Triticum durum ; durum wheat ; drought tolerance ; germination ; seedling growth ; water stress selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A series of experiments was performed in order to evaluate the significance of seed germination and seedling growth in osmotic media as screening methods for drought tolerance. Ten spring wheat (Triticum aestivum L. em Thell.) and one durum wheat (Triticum durum Desf.) were tested under controlled environments, using polyethylene glycol-6000 (PEG) solutions as the moisture stress inducing media. Tolerance in the rate of endosperm utilization, under stress, prior to the onset of germination varied among cultivars. Germination rate or injury to germination at various concentrations of PEG differed significantly among cultivars. Cultivar rating with respect to injury to germination changed with stress levels. Injury to germination did not correlate with endosperm utilization rate in PEG or in water. Germinating seedlings were tolerant to extreme desiccation up to the stage of emergence of the first leaf from the coleoptile. Growth of photosynthesizing seedlings was monitored as they were carried through an increasing concentration gradient of PEG solutions, ranging from −5.9 to −11.3 bars of water potential. Cultivars significantly differed in seedling growth tolerance to increasing levels of water stress. Seedling growth tolerance across cultivars was not correlated with their germination responses under srress. It is concluded that tolerance to water stress in growing seedlings can be screened for by using PEG-containing nutrient solutions. It can not be predicted from germination tests in osmotica. Work was done under a US-Israel Binational Science Foundation (BSF) Grant no. 1654/78.
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  • 88
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    Euphytica 29 (1980), S. 769-775 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Puccinia recondita ; leaf rust ; host-pathogen specificity ; disease resistance ; leaf rust genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Specific host-pathogen relationship is used to derive genetic information for resistance in commercial cultivars. Twenty-two cultivars were classified into 12 groups based on their reactions to 13 leaf rust (Puccinia recondita) races of India. The cultivars in each group were matched with the Lr gene carrying lines to see which genes they might possess. Confirmation of this information was sought through pedigree analyses. (1) Agra local and NP4 do not seem to have any resistance genes. (2) C306 has gene Lr14a, and NP824 one of the genes Lr12, Lr13, Lr14a or Lr22. (3) kalyansona carries Lr13 and another additional gene not in study. (4) Chhoti Lerma, NP852, Pusa Lerma, Sharbati Sonora, Shera, UP301 form one group and carry Lr1. (5) Sonalika seems to have Lr2a, Lr11 and additional genes. (6) Hy.65 has Lr10. (7) HS1076-2 and HW135 have the genes Lr2a and Lr3do. (8) HW124 carries the genes Lr1 and Lr3do. (9) Safed Lerma has Lr1 and Lr17. (10) NP846 has the genes Lr1 and Lr15. (11) HB117-107, Janak, UP215 form one group and possess the genes Lr3do and Lr15. (12) Girija possesses the genes Lr10 and Lr15. Based on such grouping of commercial cultivars for resistance genes a ‘Catalogue’ system is advocated for the design of wheat breeding programmes like the development of multiline and multigene cultivars.
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  • 89
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; crossability ; pistils ; pollen tubes ; seed set
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Different wheat genotypes (T. aestivum) were crossed with rye to ascertain the site or sites of manifestation of the crossability genes, Kr 1 and Kr 2. By using fluorescence microscopy, it was found that the order of increasing proportion of wheat micropyles containing pollen tubes is strongly correlated with the levels of crossability with rye. High crossable genotypes have more micropyles containing pollen tubes than those of the low crossable ones. Most of the inhibition or retardation of pollen tubes occurred between the style base and top of the embryo sac, expecially with the low crossable genotypes where both Kr-genes are present. The results also indicate that Kr 1 is a greater inhibitor than Kr 2. Seed set is also highly correlated with the number or proportion of micropyles having pollen tubes. Alternate pollinations seem to support the view that rye pollen tubes do not reach the micropyles of the low crossable genotypes, and hence when repollinated with wheat selfed seeds are produced.
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  • 90
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; gibberellin insensitivity ; cultivar classification according to Rht genotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The two semi-dwarfing genes Rht1 and Rht2 from Norin 10 have now been incorporated in successful varieties in use in most major wheat growing areas. The more potent dwarfing gene, Rht3, from Tom Thumb has been used in a limited way. These genes may be identified and classified by assessing the associated character of GA-insensitivity in the progeny from test crosses. This paper describes these classifications in the CIMMYT, Mexican, PBI, Cambridge and Indian breeding programmes and for a number of other international varieties.
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  • 91
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum turgidum ; durum wheat ; endosperm peroxidases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A simple method is proposed to distinguish hexaploid (Triticum aestivum L.) from tetraploid (Triticum turgidum L., durum wheat) cultivated wheats on the basis of peroxidase isozymes coded by genome D. It can also be used as a first step to detect possible contamination by tetraploid genotype mixtures. The peroxidase patterns of endosperm and of embryo plus scutellum found among 349 entries of a durum wheat world basis collection are shown.
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  • 92
    ISSN: 1573-5060
    Keywords: Wheat ; Triticum aestivum ; wheat breeding ; selection ; grain yield ; harvest index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The relationships between the F2, F3, F4 and F5 generations for grain yield were determined using random, pedigreed lines derived from each generation. The lines from two crosses were grown in plots at two sites over two years. In the first year, only F2 and F3 derived lines were available, but in the second year the F2 to F5 were grown. Correlations between lines in one generation and the mean of lines derived from them in a following generation increased as the generations were advanced. Correlations between consecutive generations were higher than those between generations two or three apart. Correlations between F2 and F5 derived lines, which indicate the effectiveness of selecting F2 lines, varied from 0.10 ns to o.49** when lines from both generations were grown in the same environment. Correlations between years of lines from the same or different generations were low and often non-significant. Harvest index was measured on the F2 and F3 derived lines at the one site in the first year. Selection for improvement of grain yield using harvest index was no more effective than selection for yield directly, when considered across years. It is conclued that, while gains in yield can be achieved by selecting for yield in early generations, a foremost consideration needs to be the influence of different sites and years on the effectiveness of selection.
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    Euphytica 30 (1981), S. 467-481 
    ISSN: 1573-5060
    Keywords: Wheat ; Australia ; Mexico ; morphology ; physiology ; numerical classification ; ordination ; time trend
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plants of 43 Australian wheat cultivars of historical and contemporary importance and 7 Mexican introductions were grown in a glasshouse and measured for 27 morphological and physiological attributes. Phenotypic relationships among the cultivars across all attributes were examined by hierarchical classification and ordination procedures. Seven major groups of cultivars delimited in the classification were broadly related to extremes of plant type (3 tall, late-flowering Australian wheats and 2 early-flowering Mexican wheats of high harvest index were contrasting extremes), region of breeding origins (southern/western versus northern Australian cereal regions) and pedigree (strong influences of the Norin 10 x Brevor cross in some Mexican cultivars and Mexican derivatives; of Federation and Early Gluyas in the pedigrees of southern/western wheats; and of Gabo in the northern wheats). Some attributes of the Australian cultivars were correlated with the year of release suggesting the progressive development of cultivars that are shorter. earlier to ear emergence and with a high harvest index.
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  • 94
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    Euphytica 30 (1981), S. 247-252 
    ISSN: 1573-5060
    Keywords: Wheat ; Triticum boeoticum ; wild einkorn ; Triticum monococcum ; einkorn ; water stress ; leaf water potential ; photosynthesis ; domestication ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A comparative study of photosynthetic response to water stress was conducted with one genotype of wild einkorn (Triticum boeoticum, W) and one of domesticated einkorn (T. monococcum, C). Per unit leaf area, W showed a better performance for photosynthetic and transpiration activities, even under dry air and dry soil conditions. Its leaf water potential was always higher than that of C at any level of soil water potential. The difference in photosynthetic recovery from severe drought between W and C was also obvious. The photosynthetic rate, transpiration rate and water status of the leaves observed at 20 h after rewatering was almost the same as non-stressed leaves in W, whereas in C the photosynthetic rate was about half that of the non-stressed leaves, which was accompanied with a low transpiration rate and a high gas diffusion resistance. The ability of W to maintain a proper water balance over a wide range of soil water potential and to recover rapidly from severe drought seems to be a result of adaptation to its hard habitats. However, under favourable water supply, the photosynthetic rate per unit leaf nitrogen was higher in C than in W. This may be advantageous to bring about a better plant growth than W on the arable land where the improved water supply is guaranteed.
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  • 95
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Triticum turgidum ; durum ; wheat ; alcohol dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Analysis of individual parts of Triticum aestivum L. and T. turgidum var. durum kernels showed two classes of alcohol dehydrogenase patterns: 1) A three banded pattern (ADH-1, ADH-2 and ADH-3) for endosperm (Ed) and 2) a seven banded pattern (ADH-F1, ADH-F2, ADH-1, ADH-2, ADH-3, ADH-S1 and ADH-S2) for embryo (E), scutellum (S) and embryo plus scutellum (E+S). The use of nullitetrasomic and ditelosomic series of Chinese Spring wheat allows alcohol dehydrogenase to be associated with arms 4Aα, 4BL and 4DS. A five subunit forming dimer hypothesis is postulated to explain the newly reported seven isozyme pattern. Subunits α, β and δ are coded by chromosome arms 4Aα, 4BL and 4DS respectively, and the newly postulated ɛ and γ are coded also by chromosome arm 4BL.
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  • 96
    ISSN: 1573-5060
    Keywords: Wheat ; Triticum aestivum ; wheat breeding ; selection ; grain yield ; harvest index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Aspects of selection for yield and harvest index were investigated by simulating selection using data from random pedigreed F2, F3, F4 and F5 derived lines from two crosses grown in plots at two sites over two years. Improvement in yield through selection was obtained when the response was measured at the same site and in the same year as the selection. Selecting the best 10 per cent of F2 to F4 derived lines gave F5 derived lines that outyielded random selections by 19 to 53 per cent for one cross and 5 to 23 per cent for the second cross. These lines were 41 to 50 per cent better than the mid-parent in one cross, but were less than the mid-parent in the other cross. However, the response to selection when measured in a different year was little better than random selection. The effect of different sites also reduced the effectiveness of selection. Selection of harvest index in early generations for improvement of yield was ineffective when response was measured at the same site in the same year, or in different years. Contrary to some theoretical proposals, the same improvement in yield was obtained by selecting in early or late generations. While high yielding genotypes may be lost by delaying selection, this is counteracted by the better predictive value of late generations due to their greater homozygosity and homogeneity.
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  • 97
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; hybrid wheat ; cytoplasmic male sterility ; partial fertility restoration ; Triticum timophecvi ; Puccinia graminis ; stem rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Many conventional hard red spring wheat (Triticum aestivum L. em Thell) lines, including several North Dakota cultivars, carry a gene (or genes) which restore partial male fertility to male sterile plants with Triticum timopheevi Zhuk. cytoplasm. Since this gene has no fertility restoration function in T. aestivum cytoplasm, the postulation can be made that it is being retained in conventional lines because of pleiotropic effects, favorable linkages or chance. The research reported in this paper examined these possibilities. Forty F6 lines, derived from a single F2 plant which was heterozygous for a gene (or genes) for partial fertility restoration, were evaluated for two years in a yield trial planted at Fargo, North Dakota. The 40 lines were testcrossed to a male sterile line having T. timopheevi cytoplasm, and the mean seed set of testcrosses was used as a measure of a line's fertility restoration potential. Twenty-seven lines had the gene for partial fertility, and 13 lines apparently lacked this gene. The 40 lines differed for heading date, anther extrusion, plant height, grain yield, 200-kernel weight, test weight, and grain protein percentage. However, comparisons of lines having the restorer gene with those lacking the gene did not provide any obvious explanation for the retention of the partial fertility restorer gene in the breeding stocks of the North Dakota conventional hard red spring wheat breeding program. The possibility that the restorer gene was linked with genes for resistance to stem rust or leaf rust also was evaluated by testing lines for their reaction to several races of rust. No conclusive association was found.
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  • 98
    Electronic Resource
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    Euphytica 31 (1982), S. 549-564 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; grain yield ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four selection experiments were sown: single-row plots with single row-spacing (≃20 cm), single-row plots with double row spacing (≃40 cm), three-row plots and six-row plots both with single row-spacing (≃20 cm). Selection entries were mimiced by 16 different varieties or advanced breeding lines, which were also sown in a yield trial. Each experiment was laid out as a 4-times replicated randomized block design. Row length was 2 m. Alley borders and border-rows of multiple-row plots were harvested separately to evaluate the effects of different harvesting procedures on the selection efficiency. Removal of alley borders was found to be disadvantageous, since the gain in precision was more than offset by the loss in sampled area. Wide spacing of single-row plots improved the selection efficiency in comparison with normal spaced single-row plots. In multiple-row plots the selection efficiency was not improved by harvesting only the central rows. For gross plot yield (= yield of net plot + yield of alley borders) the differences in selection efficiency between the various selection plot-types were explained on the basis of the genetic variance, the environmental variance and the coefficient of genetic correlation with ‘farm’ yield as determined in the yield trial.
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  • 99
    Electronic Resource
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    Springer
    Euphytica 32 (1983), S. 217-223 
    ISSN: 1573-5060
    Keywords: Hexaploid triticale ; Secale cereale ; rye ; Triticum aestivum ; wheat ; heterochromatin ; chromosome association
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the hexaploid triticale (× Triticosecale Wittmack) cultivar Rosner chromosome 2R lacks the prominent heterochromatic bands of both telomeres. This modified 2R chromosome is capable of pairing in a high frequency with wheat chromosomes. It is hypothesized that the accumulation of heterochromatin at the telomeres of rye chromosomes may have contributed to the isolation of the wheat and rye genera by inhibiting pairing between wheat and rye chromosomes.
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  • 100
    Electronic Resource
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    Springer
    Euphytica 32 (1983), S. 431-438 
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Triticum aestivum ; wheat ; T. turgidum ; durum wheat ; X Triticosecale ; triticale ; salt tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Saline soils are typically very patchy in their salinity. The yield of crops growing on them is similarly patchy. This paper argues that because most of the yield from such soils comes from the least saline areas, the best breeding strategy for improving the overall yield of crops growing on them is to select for high yield on non-saline soils. This conclusion derives from comparing the effects that four different breeding goals, namely: (1) a 10% increase in yield on non-saline soils, (ii) a 20% increase in the threshold salinity that first reduces yield, (iii) a doubling of yield at an electrical conductivity of the saturation extract (ECe) of 20 dS/m and (iv) a combination of (i) and (iii), would have on total yield. The effects of achieving these goals in barley, common wheat, durum wheat and triticale in fields exhibiting different salinities are predicted from actual yields of these species grown on different salinities in the field.
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