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  • Immunocytochemistry  (149)
  • 42.60  (90)
  • Springer  (239)
  • American Chemical Society
  • Annual Reviews
  • 1980-1984  (239)
  • 1960-1964
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  • 1
    ISSN: 1432-0649
    Keywords: 32.80 ; 42.60 ; 6.30
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The velocity distribution of evaporated Zr atoms has been measured by means of laser-induced fluorescence using a cw dye laser by scanning the laser line across the Doppler-broadened absorption line profile of the atoms. It is shown that the experimental data can be explained on the basis of an excitation theory for a three-level system developed from basic principles. It was calculated that the probability for an atom to be in the excited state mainly depends on the radiation-power density of the laser and on the residence time of the atom in the exciting laser light. It was found that the laser frequency must be well stabilized in order to meet the assumptions in the excitation calculation. Otherwise deviations to the theoretical predictions appear in the experimental results due to the jitter of the dye laser.
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  • 2
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    Applied physics 35 (1984), S. 123-126 
    ISSN: 1432-0649
    Keywords: 42.50 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Spatial separation of wavelengths in a CO2-laser resonator helps to avoid competitions. We have achieved this by means of a nearly concentric resonator with a grating or prism in its center. Thus, we demonstrated independent multiline emission with a variety of output spectra. The temporal sequence of the lines was conveniently controlled by varying their losses.
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  • 3
    ISSN: 1432-0649
    Keywords: 42.60 ; 07.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
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  • 4
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    Applied physics 30 (1983), S. 135-142 
    ISSN: 1432-0649
    Keywords: 42.55 ; 42.60 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract An analysis of cw synchronously pumped dye lasers is presented. Under the assumption that the cavity (tuning element) bandwidth is much wider than the bandwidth of the transform limited pulses generated, the pulse forming dynamics is rigorously treated. It is shown that for a finite mismatch between the lengths of the dye and the pump lasers, a steady-state pulse develops in the dye laser cavity with a conserved pulseshape. The characteristics (energy, shape, peak power, duration) of these pulses of ultimate width are quantitatively determined as a function of cavity mismatch. An analytical solution for the pulse envelope is determined, which yieldsI(t)∝Sech2(t/t p ) to a good approximation.
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  • 5
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    Applied physics 30 (1983), S. 161-176 
    ISSN: 1432-0649
    Keywords: 32 ; 42.50 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A new method for determining trace amounts of elements in a substance has been developed. It is based on thermal evaporation and atomization of a substance in vacuum and detection of the released trace atoms by the laser stepwise photoionization technique. A rather effective way of detecting atoms is their multistep excitation to Rydberg states by the radiation of tunable dye lasers with their subsequent ionization by electric field pulses. The analytical laser spectrometer consisting of several tunable, pulsed dye lasers, a vacuum chamber with an atomizer and a recording system is described. The microimpurities of elements have been detected in pure materials: the contents of Na impurities in CdS crystals (2×10−6%) and Al impurities in germanium (2×10−7%). The detection limit of about 10−9% was determined. A technique for direct determination of the content of elements in natural water and human blood is described. A high sensitivity, universality and other merits of the method enable it to be used in determining traces of many important elements at a level of 10−8 to 10−11% almost in any matrix.
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  • 6
    ISSN: 1432-0649
    Keywords: 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We have shown that distributed feedback dye lasers are capable of generating single transform-limited pulses of 1.6–3.5 ps duration when pumped by a mode-locked Nd: YAG laser. A short-pulse amplifier has been constructed in which the level of amplified spontaneous emission could be kept below 10−3 by properly taking into account the polarization anisotropy of the gain and by precise timing of the pump pulses. A sensitive autocorrelator is described which allows to measure pulse duration in a single shot.
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  • 7
    ISSN: 1432-0649
    Keywords: 42.60 ; 42.65 ; 61.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Absorption recovery kinetics data on the picosecond time-scale of a high signalto-noise ratio can be obtained by using continuously operating synchronously modelocked dye lasers. We show that such data make it possible to determine multiple exponential decays with small differences in rate constants. We also show that an absolute prerequisite to accomplish this is that the measurements are performed at a number of different wavelengths, and that the decays are monitored in a time interval corresponding to several lifetimes. These considerations are demonstrated by measurements on two carbocyanine dyes, DQOCI and pinacyanol, respectively.
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  • 8
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    Applied physics 32 (1983), S. 127-135 
    ISSN: 1432-0649
    Keywords: 42.55 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A cw uv copper hollow cathode laser is described, which could be operated for a longer time duration with an output power of 7 mW in the 260 nm region. A double-cathode configuration with a protection for the mirrors from metal-vapour deposition is presented. Characteristics of the laser output power, the spontaneous emission, and the ion densities in the negative glow were measured as depending on the discharge current, the neon gas pressure, and the size of the cathode slit. The observed saturation of the laser output power at high discharge current (1 A/cm2) and the optimum slot size are discussed.
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  • 9
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    Applied physics 33 (1984), S. 23-27 
    ISSN: 1432-0649
    Keywords: 42.55 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract For the first time stable cw output of a FIR-CO2 hybrid laser has been achieved at wavelengths shorter than 300 μm. Due to the saturable absorber characteristic of the FIR laser gas, cw or pulsed emission is observed on both, the CO2 and the FIR laser output, depending on the operating conditions. Results are reported on different resonant lines in CH3OH and the 67 μm Raman line in NH3. The good efficiency of this laser is also demonstrated by the excitation of two new emission lines in CH3OH, namely 49 and 56 μm, pumped by 9R(22) and 9R(24), respectively.
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  • 10
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    Applied physics 33 (1984), S. 57-61 
    ISSN: 1432-0649
    Keywords: 33 ; 42.60 ; 82.50
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A laser photodesorption microscope has been applied to obtain 4×103-fold images of rhodamine crystals on a tip with a diameter of 66μm. Peculiarities of the formation of these images have been studied with different values of electric field strength on the tip and laser pulse intensities.
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  • 11
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    Applied physics 33 (1984), S. 179-185 
    ISSN: 1432-0649
    Keywords: 06 ; 07.60 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We describe a study, performed using heterodyne techniques, of the frequency fluctuations of two completely independent ring dye lasers locked to independent reference cavities. Single laser linewidths of less than 750 Hz were achieved, the principal limitation being residual vibrations from the noisy laboratory environment. With future design and environmental improvements, ultranarrow linewidths are expected thus providing a useful tool for a great variety of high precision experiments.
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  • 12
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    Applied physics 33 (1984), S. 213-218 
    ISSN: 1432-0649
    Keywords: 35 ; 82.40 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract CARS spectra of NH3 and the free NH2-radical in the NH stretch region at 3334 and 3220 cm−1 have been obtained, respectively. The NH2 radicals were generated by laser photolysis of 0.5 mbar NH3 at 193 nm with an ArF-exciplex laser and detected by time resolved CARS spectroscopy.
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  • 13
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    Applied physics 34 (1984), S. 63-67 
    ISSN: 1432-0649
    Keywords: 06 ; 07.60 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract An infrared frequency measurement chain is reported, the simplicity of which allows phase-coherent frequency measurements up to 30 THz. Frequency measurement is demonstrated on the 10P(28) line of the Lamb-dip stabilized13CO2-laser.
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  • 14
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    Applied physics 34 (1984), S. 129-137 
    ISSN: 1432-0649
    Keywords: 33.50 ; 42.10 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A theoretical description of a three-pulse transient grating technique is given where the pump pulses form orientational gratings. Including energy relaxation and angular reorientation the temporal behavior of the diffraction efficiency and energy transmission is discussed with respect to the dependence on the corresponding rate constants and the results are compared with experimental data for Rh6G and RhB. The polarization plane of the pulse diffracted by the grating is found to be rotated in dependence on its initial polarization.
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  • 15
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    Applied physics 35 (1984), S. 163-166 
    ISSN: 1432-0649
    Keywords: 42.50 ; 42.55 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We present an analytical expression for the small-signal gain of a Free Electron Laser (FEL) in the presence of a Gaussian mode. To describe the electron beam evolution we use the one-dimensional (1-d) Vlasov equation. Our perturbation result, valid for small values of the parameterq (length of the undulatorL divided by the Rayleigh rangeZ R), can be extrapolated to values ofq≈4÷5.
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  • 16
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    Applied physics 29 (1982), S. 93-97 
    ISSN: 1432-0649
    Keywords: 33 ; 42.60 ; 82.40
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Simultaneous, spatially resolved monitoring of C2 and OH in a flame is reported. Data could be captured in 10 ns employing two pulsed lasers and a diode array detector. Laser-production of C2 was also observed and a kinetic study of such molecules was performed.
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  • 17
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    Applied physics 32 (1983), S. 119-122 
    ISSN: 1432-0649
    Keywords: 42.80 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The model which reproduces accurately and conveniently the waveform of the amplitude modulated cw laser light in the TEM00 mode by a mechanical chopper is developed. It is found that its predictions are in good agreement with the strict, completely accurate but rather lengthy and inconvenient treatment and with the experiment. The criterion for the efficient amplitude modulation of the laser light by a mechanical chopper is formulated.
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  • 18
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    Applied physics 33 (1984), S. 63-74 
    ISSN: 1432-0649
    Keywords: 42.60 ; 78.30 ; 78.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract This review covers the field of vapor-phase dye lasers (VPDL) with special emphasis on prospects of gas lasers with optical pumping. Some peculiarities of the gaseous active medium and a possibility to control its density near the critical point are discussed. High specific laser energies (∼0.3J/cm−3), low thresholds, and high efficiencies (20–40%) made it possible to construct a powerful tunable VPDL.
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  • 19
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    Applied physics 33 (1984), S. 103-113 
    ISSN: 1432-0649
    Keywords: 42.68 ; 42.80 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A long-path absorption procedure for the determination of tropospheric OH concentrations is described in detail. Initial measurements using this method were carried out in Frankfurt a. M. By including in the evaluation the visible radiation of a frequencydoubled dye laser used in the measurements, it was possible to mathematically reduce the signal fluctuations caused by the scattering and density fluctuations of tropospheric air. The resulting detection limit was 3×106 OH per cm3. SO2 concentrations which had to be simultaneously determined in order to eliminate an interference effect, could be detected in the range of 1–40 ppb on the absorption path.
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  • 20
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    Applied physics 31 (1983), S. 15-17 
    ISSN: 1432-0649
    Keywords: 35 ; 07.65 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract An absorption method which permits time-selective measurements at different wavelengths has been developed in order to explore magnetic field effects on photoinduced electron transfer reactions in linked and unlinked donor-acceptor systems.
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  • 21
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    Applied physics 32 (1983), S. 7-8 
    ISSN: 1432-0649
    Keywords: 42.60 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Bistable operation of a dual-wavelength synchronously mode-locked cw dye laser is reported. Wavelength switching is found to depend on the pump power and the dye laser cavity lengths.
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  • 22
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    Applied physics 33 (1984), S. 9-16 
    ISSN: 1432-0649
    Keywords: 42.60 ; 42.20 ; 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We derive the most general equations of motion for the electrons and the electromagnetic field in a free-electron laser including the effects of diffraction and pulse propagation. The field evolution is expressed in terms of the amplitudes and phases of a complete set of transverse modes. The analytic solution is given in the small-signal regime, where the theory is shown to be in excellent agreement with a recent experiment at Orsay.
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  • 23
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    Applied physics 33 (1984), S. 83-90 
    ISSN: 1432-0649
    Keywords: 82.50 ; 82.40 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Tritium isotope separation by CO2-laser induced multiphoton dissociation of CTF3 is investigated. For the optimization of the performance of this working substance, trifluoromethane, the conditions to yield high-selectivity at high-operating pressure and low-critical fluence for complete dissociation are studied using our deconvolution procedure. The irradiation conditions are varied over the following ranges; wavenumber: 1052–1087 cm−1, gas temperature: 25°C to −78°C, CHF3 pressure: 5–205 Torr. The selectivities exceeding 104 are observed for 85–205 Torr CHF3 at −78°C by the irradiation at 1057 cm−1.
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  • 24
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    Applied physics 34 (1984), S. 29-31 
    ISSN: 1432-0649
    Keywords: 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Directional and wavelength sweep of a pulse train from a distributed-feedback dye laser is studied and found not to be a thermal effect, rather a gain-induced one. The directional sweep can be used for single-pulse selection.
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  • 25
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  • 26
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    Applied physics 30 (1983), S. 29-33 
    ISSN: 1432-0649
    Keywords: 42.55 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract This report describes an experimental examination of the output characteristics of the continuous-wave (cw) carbon monoxide flame chemical laser (FCL) of the CS2/O2/N2O type in case of small CS2/O2 reactants ratios (tipically CS2/O2≦1/10). A linear burner which gives a homogeneous and stable flame was used during the experimental study. The measurements of temperature distribution in CS2/O2 as well as CS2/O2/N2O flames show maximum temperatures of 1040 and 890 K, respectively. The addition of nitrous oxide (N2O) leads to dramatically enhanced output laser power caused primarily by V−V transfer processes. A chemical efficiency, based on the reaction O+CS→CO*+S, of 3% was achieved. The spectral composition of the CO FCL of the CS2/O2/N2O type shows lasing in the region from 5.130 to 5.586 μm. Experimental results were obtained with a nondispersive optical cavity.
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  • 27
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    Applied physics 29 (1982), S. 19-21 
    ISSN: 1432-0649
    Keywords: 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A continuously operating jet stream dye laser was pumped by a multiwavelength-multimode argon-ion-laser with a pump power up to 200 W. Rhodamine 6G-chloride was dissolved in a water-polyvinylalcohol-ammonyx solution. The dye laser was prism-tunable between 560 and 650 nm with maximum output power at 600 nm. The maximum output power of the cw dye laser was 43 W without tuning element and 33 W with a prism placed inside the cavity. Operation of a jet stream dye laser with a pump power higher than 200 W and an efficiency of about 30% seems to be possible.
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  • 28
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    Applied physics 29 (1982), S. 157-158 
    ISSN: 1432-0649
    Keywords: 42.60 ; 42.55
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    Topics: Physics
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  • 29
    ISSN: 1432-0649
    Keywords: 42.60 ; 42.55
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    Topics: Physics
    Notes: Conclusion We have demonstrated the existence of an anomalous polarization coupling mechanism present in the injection locked TEA CO2 laser. As a direct consequence of this work, a new injection technique has been formulated which permits efficient injection coupling and maximises energy extraction whilst eliminating the detrimental optical feedback effects to which other injection locking systems are prone.
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  • 30
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    Applied physics 35 (1984), S. 71-75 
    ISSN: 1432-0649
    Keywords: 42.60 ; 42.55 ; 42.72
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A simple model of the energy transfer between a booster dye and the active dye in a flashlamp pumped planar waveguide dye laser is described. Analytical results are compared with experiments for several dyes in the 600–650 nm region. A twofold increase of laser output was found at a wavelength (630 nm) which is of particular interest for several applications.
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  • 31
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    Applied physics 35 (1984), S. 119-122 
    ISSN: 1432-0649
    Keywords: 42.72 ; 42.80 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The output of a CO2 laser, operating on theP I(18) transition of13C16O2 at 26941 GHz (11.128 μm) was phase-locked to a 5 MHz signal from a primary Cs frequency standard by means of a frequency chain having only CO2 lasers as infrared sources. Simultaneously, four other CO2 lasers in the chain were phase-locked to the 26941 GHz output. This provided CO2 laser frequencies at 26 450 305, 26 940 815, 28 694 625, 29 442 480, and 33 185 715 MHz having zero long-term-average frequency error relative to the Cs standard, and the ±10−13 (≃3 Hz) long-term absolute uncertainty of the standard.
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  • 32
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    Applied physics 33 (1984), S. 187-193 
    ISSN: 1432-0649
    Keywords: 42.50 ; 42.55 ; 42.60
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    Topics: Physics
    Notes: Abstract It is shown by numerical analysis based on Lamb's equations of motion, that standing-wave lasers with purely homogeneously broadened emission lines exhibit regular multimode oscillations. Specifically, modes lying far from the line centre are quenched due to mode competition, and the amplitudes of the oscillating modes approach steady-state values. The stabilization of the amplitudes is normally accompanied, or followed, by an evolution of the phases towards a phase-locked regime, where the relative phasesψ n = 2φ n −φ n + 1 −φ n − 1 [φ n phase in then th mode, defined by (6)] attain either the value 0 or ± π. The build-up times for the relative phases are found to vary over a wide range.
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  • 33
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    Applied physics 33 (1984), S. 219-223 
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    Keywords: 42.60
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    Topics: Physics
    Notes: Abstract We describe the generation of excimer-laser pulses of 〈10 ps pulse width and up to 40 mJ pulse energy at 248.5 nm and their use in the measurement of ps gain dynamics in a KrF amplifier. Small-signal gain of 〉2×104, saturation energy density of 2.0 mJ/cm2, and gain recovery time of 4 ns were measured. In contrast to XeCl* no short-gain rcovery time was found in KrF* and the stored inversion could be fully depleted bya single ps pulse.
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  • 34
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    Applied physics 26 (1981), S. 227-229 
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    Keywords: 42.60 ; 34.70
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    Topics: Physics
    Notes: Abstract Near-infrared laser action of nitrogen atoms was obtained in a hollow cathode discharge. Four laser lines at wavelengths of 1358.2, 939.3, 938.7, and 862.9 nm were observed in the cw mode. In addition, the laser line at 904.6 nm was observed in quasi-cw operation in the afterglow for the first time. It is supposed that resonant charge exchange between He+ and N2 followed by predissociation of N 2 + is an important way of populating the upper laser levels.
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  • 35
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    Applied physics 27 (1982), S. 27-37 
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    Topics: Physics
    Notes: Abstract The circulation of a synchronously mode locked dye laser pulse in a linear cavity configuration containing a dye cell as an active medium and a bandwidth-limiting element is treated. The condition that the pulse shape reproduces after each cavity round-trip provides a nonlinear integro-differential equation for the steady-state pulse shape. For the solution of this equation an approximate method, not limited to small pulse energies, is given and the pulse duration, intensity, energy, asymmetry of the pulse shape, stable regions and other interesting parameters are discussed.
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  • 36
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    Keywords: 68 ; 42.70 ; 42.60 ; 61.70 ; 61.50 ; 42.80 ; 29
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  • 37
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    Applied physics 28 (1982), S. 319-326 
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    Keywords: 07.60 ; 07.65 ; 42.60
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    Topics: Physics
    Notes: Abstract A sync-pumped cw dye laser system has been used to produce subpicosecond pulses. Pulses as short as 0.7 ps, assuming a single-sided exponential pulse shape, were observed. A set of experiments was performed to investigate the origin and effects of noise in the sync-pumped system. A digital and an analog feedback loop have been designed to optimize the pulse width. The noise has been lowered by 10 dB for frequencies up to 10 kHz; long-term drift is also controlled by this method. A four-stage dye laser amplifier, pumped by a Nd:YAG laser which operates at a 10-Hz repetition rate, is synchronized electronically to the dye-laser picosecond pulses. A gain of 3×106 has been achieved.
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  • 38
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    Applied physics 29 (1982), S. 111-115 
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    Keywords: 42.60 ; 34.70
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    Topics: Physics
    Notes: Abstract C, N, O, F, Cl, and Br red and infrared laser emission was investigated using hollow cathode discharges in gas mixtures of helium and molecules containing the corresponding atoms. A total of 33 laser lines with wavelengths from 0.7 to 2.0μm was observed. Three laser lines of atomic C, five laser lines of atomic N, two laser lines of atomic Cl and five laser lines of atomic Br were observed for the first time. Dissociation charge transfer and dissociative excitation transfer are suggested as being responsible for populating the upper laser levels.
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  • 39
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    Notes: Abstract The initial stage of thermochemical instability induced by an absorbing inclusion within a transparent matrix, which irradiated by a laser beam, is studied. The instability results in a low threshold (formally non-threshold) optical breakdown of the matrix. It is brought about by the additional absorption of the laser light by the products of the chemical reactions in the matrix. The instability is characterized by a certain induction time when the rate of the chemical reactions is almost steady. When the induction is over, their rate rapidly increases. Theoretical estimate of the induction time are offered. The experimental study of the induction time dependence on the laser-beam intensity confirmed the theoretical estimation.
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  • 40
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    Notes: Abstract Isotope separation of tritium from deuterium in heavy water was attempted by CO2-laser-induced, highly-selective multiphoton dissociation of C2TF5 present in C2DF5. Single-step T/D separation factors exceeding 3000, 1000, and 500 were attained, respectively, for the first time with CO2 laser 10P(34) 931.0 cm−1 at 10, 20, and 30 Torr pentafluoroethane pressures at −78 °C (i.e., equivalent to 15, 30, and 45 Torr at room temperature).
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    Applied physics 27 (1982), S. 105-113 
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    Notes: Abstract The circulation of an ultrashort light pulse in a continuously pumped modelocked dye laser with a linear cavity configuration containing the active dye, the saturable absorber and a bandwidth-limiting elements is treated. The steady-state condition that the pulse shape reproduces after each cavity round-trip leads to a nonlinear integro-differential equation for this pulse shape. An approximate method for the solution of this equation not limited to the case of low laser gain and small pulse energies is given. The stable single pulse region and characteristic pulse parameters, as energy, duration, intensity and asymmetry, are considered in dependence on the laser parameters.
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  • 42
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  • 43
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    Applied physics 28 (1982), S. 341-348 
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    Notes: Abstract Isotope separation of tritium by CO2 laser-induced multiphoton dissociation (MPD) of C2TF5 is reported for the first time. The MPD spectrum obtained for C2TF5 comprised a broad peak at about 940 cm−1 where C2HF5 was nearly transparent. The unimolecular dissociation of C2TF5 was induced with much lower laser fluence than that for CTF3, another working molecule we proposed for laser isotope separation of tritium. The mechanisms and kinetics of the dissociation of C2TF5 and C2HF5 were investigated under various experimental conditions: laser frequency, pulse energy, pulse duration, tritium concentration, sample pressure, buffer gas pressure and irradiation geometry. Single-step separation factors exceeding 500 were achieved with the most efficientP(20) line in 00o–10o0 transition at 944.2 cm−1.
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  • 44
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    Notes: Abstract An analysis of 532 nm lidar data is presented for the retrieval of vertical extinction profiles. The strong influence of the relative humidity on the extinction-to-backscatter ratio is parametrized for this purpose. A comparison is made between remotely sensed and locally measured extinction coefficients, using reference values in aircraft and at ground level.
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  • 45
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    Applied physics 34 (1984), S. 191-192 
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    Notes: Abstract A pulsed xenon ion laser has been used to pump a rhodamine 6G dye laser utilizing a ring resonator. The dye laser has been passively mode-locked; a pulsewidth of 0.5 ps and a 50 kW peak power have been obtained.
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  • 46
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    Notes: Abstract The values of the chopping disc slot and mark space widths relative to the radius of the Gaussian beam are found, which achieve optimum harmonic-like and efficient amplitude modulation of the cw laser beam. The simple approximation for the waveform of the modulated laser power valid for these optimum or near optimum values of the chopping disc parameters is presented.
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  • 47
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    Notes: Abstract A time standard based on the use of an optical oscillation period of a frequencystable He−Ne laser as a time scale is first described. We obtained highly frequency-stable oscillations in the SHF range that were locked to the oscillations of a He−Ne laser stabilized to an absorption resonance in methane at 3.39 μm. A direct comparison of frequency stabilities of a rubidium standard and He−Ne/CH4 laser has been made. The absolute measurement of the frequency of the He−Ne/CH4 laser we performed gave a new value of frequency.
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  • 48
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    Keywords: 32 ; 35 ; 42.65 ; 32 ; 33 ; 42.60
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  • 49
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  • 50
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    Applied physics 32 (1983), S. 123-125 
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    Notes: Abstract Short (〈0.5 ns) ultraviolet laser pulse generation by quenching of resonator transients is described. An output energy of 100–150 nJ at λ=340 nm is obtained using a pump energy of only 5–7 mJ at 308 nm. This pulse was amplified up to 100 μJ by a two stage amplifier.
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  • 51
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    Applied physics 33 (1984), S. 17-21 
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    Notes: Abstract A comparison of the operating characteristics of the high-voltage regime of the discharge in a hollow-cathode tube, the hollow-canthode discharge (HCD) and the discharge to a plane cathode are presented. The disappearnce of the hollow-cathode effect and the transition to a high-voltage discharge after inserting several anode rods into the cathode cylinder is exhibited. The similarity between the operating characteristics of such a high-voltage discharge and of a plane cathode discharge is shown. The loss of ions at the anode rods, as well at insulators or floating conductors is believed to be the reason behind the increase of the operating voltage and the disappearance of HCD characteristics. Practical means of increasing the operating voltage are mentioned.
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  • 52
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    Applied physics 33 (1984), S. 75-77 
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    Notes: Abstract We report upon some experiments done on a continuous-wave argon-ion laser which offers 300 W output power in the visible and 34 W in the ultraviolet region of the spectrum, limited by the degradation of the resonator mirrors. To avoid destruction of the outcoupling mirror, a folded three-mirror instead of a two-mirror cavity was tried, resulting in nearly the same output power.
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  • 53
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    Applied physics 33 (1984), S. 95-98 
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    Notes: Abstract Generation of single dye laser pulses of 12 ps pulse width (FWHM) and 1.2 mJ pulse energy is described using only one XeCl-excimer laser as pump source. At 1 Hz pulse repetition rate the stability of amplitude and pulse width of these pulses is excellent.
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    Applied physics 35 (1984), S. 233-236 
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    Notes: Abstract The effect of resonant laser-light upon a charge-exchange source of eV-range sodium atoms is to decrease the Na kinetic energy by about 2 eV. The mechanism probably involves a reduction of the space charge in the low-energy ion precursors. The reduction is caused by electrons created in collisional ionization of laser-excited atoms. This is related to the well-known optogalvanic effect. A knowledge of the existence of this effect can be important for the design of experiments involving laser driven chemistry.
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    Applied physics 26 (1981), S. 251-253 
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    Notes: Abstract The problem of propagation of 10.6 μm-CO2 laser pulses in fog has been formulated and solved numerically, making use of Duhammel's principle. Fog droplets are considered Mie scatterers in the Rayleigh limit. Evaporation, power loss and time delay of pulse peak have been studied. Possible extensions of the technique have been discussed.
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  • 56
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  • 57
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    Applied physics 30 (1983), S. 5-10 
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    Notes: Abstract Bi-directional and folded laser amplifiers are analysed in the constant intensity approximation. Simple analytical expressions are developed for gain, output intensity and extraction efficiency. The analysis is applied to electron-beam pumped KrF lasers and expressions for saturation intensity, small-signal gain coefficient and intrinsic efficiency in terms of gas composition and pump power per unit volume are given. The problem of fluorine burn-up in KrF lasers is discussed. Under certain conditions the analysis is shown to be equally applicable to oscillators and comparison is made with previous experimental results and computer code predictions.
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    Applied physics 30 (1983), S. 99-104 
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    Notes: Abstract Some new experimental data on the time development of ultrashort superfluorescence (SF) in the dye solution are presented. The correlation between temporal behaviour change and the change of the spectral and spatial intensity distribution gives direct evidence of the important role of the selffocusing phenomenon in the formation of the ultrashort SF pulse synchronized with the pumping one. The oriental relaxation of solvent molecules in the dye solution not only influences the duration of the dye SF via the selffocusing mechanism but also causes a temporal change of spontaneous emission spectrum. At least two different relaxation times were found in the fluorescence spectrum of the dye in several polar solutions.
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  • 59
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    Applied physics 35 (1984), S. 199-200 
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    Notes: Abstract We report results of harmonic mixing experiments between millimetric oscillators and FIR laser radiations up to 3 THz, using micrometer-size Schottky-barrier diodes. Signal-to-noise ratios suitable for precise frequency counting have been obtained for harmonic numbers up to 40.
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  • 60
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    Applied physics 35 (1984), S. 227-231 
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    Notes: Abstract The feasibility of using the electrically excited XeF(C»A) excimer medium as an efficient wideband amplifier in the blue-green region of the spectrum has been investigated. Calculations show that for an intense blue-green optical flux input the amplification characteristics of XeF(C»A) improve, as a result of both bleaching of the pump induced absorbers and by additional production of XeF(C) level population. The removal of one of the major absorbing species, Xe** in the XeF (C»A) laser mixture by means of a pulsed ruby laser probe beam has been demonstrated.
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  • 61
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    Applied physics 31 (1983), S. 193-199 
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    Notes: Abstract It is shown that LiF crystals with F 2 − colour centers may be used in lasers for cavity Q-switching and in high-power Nd: glass laser systems for, increasing the contrast and the self-excitation threshold. Also Lif crystals with F 2 − centers are successfully used for the generation of ultrashort pulses, for the generation of difference frequencies in the ir spectral range and in intracavity spectroscopy experiments.
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  • 62
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    Applied physics 32 (1983), S. 49-52 
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    Notes: Abstract Methods for simultaneous measurements of more than one flow velocity component using coherent Raman spectroscopy are proposed. It is demonstrated that using a kilowatt broad-band probe pulse (3–30 GHz) along with a megawatt narrow-band pump pulse (∼100 MHz), coherent Raman signal resulting from a single laser pulse is sufficient to produce a high-resolution Raman spectrum for a velocity measurement.
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  • 63
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    Notes: Abstract We report the observation of coherent uv emission at 330.2±0.5 nm when sodium vapor in a heatpipe is irradiated with a pulsed visible dye laser with output wavelength in the 570–595 nm region. It is found that intense uv emission can be produced from Na atoms as well as from Na2 molecules. The excitation functions and their dependence on vapor density and laser power density are presented and the mechanisms for producing the emission are discussed.
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    Applied physics 27 (1982), S. 123-128 
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    Notes: Abstract This paper reports results of an experimental study of a cw 118.6 μm H2O waveguide laser. The laser cavity is a 3 cm inner diameter, 3.2 m long Pyrex tube, with plane reflectors against its ends, one of them being a copper mesh coupler. The active medium is a H2O:H2 gas mixture. Successive optimizations lead to a reliable output power of 50 mW, for the EH11 mode. The power density is the highest ever reported, and the stability is such that the power drifts by less than 2% per hour. This laser is competitive with the optically pumped 118.8 μm CH3OH laser.
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    Applied physics 27 (1982), S. 167-168 
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    Notes: Abstract The 11th harmonic mixing signals between a 386 GHz oscillator and a 4.25 THz laser with a signal to noise ratio of 25 dB in 100 kHz bandwidth have been obtained with a Schottky diode.
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  • 66
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    Applied physics 27 (1982), S. 191-194 
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    Notes: Abstract Aqueous solutions of benzene were irradiated by focussed KrF laser light. The formation of phenol together with varying amounts of 1.3-cyclopentadiene-1-carboxaldehyde was shown by uv absorption measurements. A mechanism for this photo-oxidation via benzene radical cations produced by 2-photon ionization of benzene is proposed. Comparative gas phase laser experiments and experiments with unfocussed laser beams were consistent with previous results using incoherent light sources.
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    Applied physics 27 (1982), S. 183-186 
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    Notes: Abstract ArF laser photolysis of acetylene at 193 nm yields diacetylene, ethylene and hydrogen (besides a polymer). No benzene or vinylacetylene is formed. Photolysis with deuterium gives no deuterated compounds. These observations are taken as evidence for a pure excited-molecule mechanism based upon C2H 2 * (1 A u).
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  • 68
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    Notes: Abstract To improve the gain in the Orsay storage ring Free Electron Laser (FEL) experiment, the 17 period permanent magnet undulator has been modified to form an optical klystron (OK). We report the measurement of spontaneous emission and the effects on it of energy spread and angular spread. Gain and laser induced bunch lengthening measurements with the OK are also reported and are in very good agreement with the FEL classical theory. The spontaneous emission spectrum which is easy to measure with good signal to noise ratio, turns out to be a very good diagnostic tool forenergy spread and angular spread measurements on storage rings. The factor of four increase in the small gain obtained by converting the undulator NOEL into an OK was the critical factor in the recent operation of the ACO storage ring laser above threshold.
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    Applied physics 35 (1984), S. 31-36 
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    Notes: Abstract A spectroscopic dye laser system-pumped by a Copper Vapor Laser (CVL)-has been built and optimized for different dye solutions operating in the spectral range from 530 to 890 nm. Conversion efficiencies up to 40% were reached in broad band operation, 24% with a prism expander grating cavity, and 20% in grazing incidence configuration, which operated at typical band widths of 3 GHz. Second harmonic generation (SHG) of the dye laser output produced tunable uv-radiation between 260 and 408 nm at conversion efficiencies ≦5%. Fundamental and SHG output were used for resonant ionization experiments in molecular beams.
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    Applied physics 32 (1983), S. 39-42 
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    Notes: Abstract System parameters of an optical parametric generator pumped by the third harmonic of a Nd:glass laser are presented. The tuning range extends from 5700 to 22,700 cm−1; i.e., it covers a frequency range of 17,000 cm−1. Experimental data on spectral bandwidths, conversion efficiencies, and pulse shape are discussed. A pump beam with a divergence of four degrees simultaneously generates the entire visible spectrum (blue to red) separated by the angle of emission.
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    Applied physics 32 (1983), S. 113-118 
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    Keywords: 42.60 ; 92.60T ; 92.65
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    Notes: Abstract Monitoring of the height distribution of atmospheric ozone up to 50 km is being performed with a ground-based lidar system, which has been in operation since October 1982 on the summit of Zugspitze in the German Alps. Daily and monthly averages of the ozone profile are being obtained with high precision. Furthermore, stratospheric aerosol layers, originating from the eruption of El Chichon volcano in Mexico in sping 1982 are being recorded.
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  • 72
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    Applied physics 24 (1981), S. 331-340 
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A full-automatic wavelength controled pulsed dye laser has been developed. With the control system, dye laser oscillations of nanosecond pulses are tuned to any wavelength in the visible or scanned in any sweep mode keeping the nearly transform-limited bandwidth of 0.45 GHz. A mechanical tuning of the wavelength is employed, such that the motion of three wavelength selectors is synchronized to give a smooth and precise wavelength control. The basic principle underlying the tuning scheme, various requirements to be met in system construction, and the practical performance of automatic wavelength control are described in this paper.
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    Applied physics 21 (1980), S. 199-204 
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    Notes: Abstract Coherent infrared radiation tunable between 1.6 and 3.2 μm was generated with a pulsed dye laser by 6s−5d 5/2 stimulated electronic Raman scattering in caesium vapour. We describe a technique using thermal dissociation to reduce the density of caesium dimers in a heat-pipe oven, which produced a factor of 7 reduction in Raman threshold. The threshold displayed a marked dependence on pump bandwidth.
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    Applied physics 21 (1980), S. 237-240 
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    Notes: Abstract Selective single-line oscillation has been obtained from an NH3 laser in the range from 11 to 13 μm. Pumping of high pressure NH3−He mixture with theR(30) line of a TEA-CO2 laser made it possible to tune on more than 30 lines with the aid of a grating. However, such line tunable oscillation was not observed when theR(16) line was used for pumping. Reasons for this difference are discussed.
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    Applied physics 21 (1980), S. 319-321 
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    Notes: Abstract We report the observation that the helical TE N2 laser can effectively be simplified by giving up the use of decoupling elements as well as by abolishing the segmentation of the electrode structure. Although, as a consequence of this simplification, the operating pressure range was slightly decreased, the output power could be improved by roughly 30%, a result which is attributed to the new electrode geometry exhibiting lower inductance and lower damping losses.
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    Applied physics 21 (1980), S. 353-359 
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    Notes: Abstract Some aspects of the transient behaviour of pulsed dye lasers are investigated both theoretically and experimentally. Relaxation oscillations induced in the output from a dye cell by an external reflector have been observed and explained, using a rate equation approximation to the physical situation in the dye cell. These oscillations are shown to play an important part in the generation of short laser pulses from long cavity dye lasers. Finally, it is proposed that 10 ps pulses can be obtained from conventional dye lasers pumped by 100 ps pulses from a high-pressure nitrogen laser.
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    Applied physics 22 (1980), S. 213-218 
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    Notes: Abstract We present periodic solutions, which are described in terms of Jacobian elliptic functions, for the steady state pulses from a laser system consisting of a pair of amplifiers of unequal relaxation time mode locked by a “fast” saturable absorber. The pulsewidth and the average power in the pulse are expressed interms of the system parameters through two different approaches, where detailed plots are given for one of the approaches. Summary of the characters to be possessed by the amplifier pair to ensure the reliable production of shortest possible pulses is given.
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    Notes: Abstract In this paper we describe the construction of a monochromatic and frequency stabilized continuous wave (cw) source in the 5 μm region. It is built up by a cw single line, single-mode frequency and amplitude stabilized CO2 laser. This primary CO2 laser source is then frequency-doubled in a tellurium crystal using second harmonic generation (SHG). The obtained emission is monochromatic (half-width 50 kHz). The frequency can be varied discreetly from 4.6 μm to 5.5 μ depending on the CO2 laser line chosen.
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  • 80
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    Notes: Abstract An investigation was made of the self-induced transparency effect in the semiconductor's exciton region using ultrashort frequency-tunable light pulses and an ultrafast streak camera.
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    Applied physics 25 (1981), S. 161-164 
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    Notes: Abstract The possibility to use narrow optical resonances and highly stable lasers as detectors of gravitational waves are discussed. The methods for measuring small displacements are based on the registration of small variations of a radiation frequency that arise in changing an optical phase. First results on the use of narrow resonances for precision measurements of small displacements are given. The achieved absolute sensitivity of the measurements amounted to 6·10−6 Å on the basis of 5·102 cm.
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    Applied physics 25 (1981), S. 311-316 
    ISSN: 1432-0630
    Keywords: 07.40 ; 07.60 ; 42.60 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A high precision wavelength meter in the visible is described, which is based on a Fabry-Perot interferometer with several etalons of different resolution. The interference fringe pattern projected on a photo-diode array detector is computationally processed to give a stepwise refinement of the wavelength value to any adjusted accuracy. The present model intends to provide digital and real-time values of high precision wavelength for dye-laser spectroscopy, and to serve as a monitor or as a pilot for wavelength control of a dye-laser source of nanosecond pulses. The model is, therefore, designed with particular emphasis on its short-pulse capability and on-line mode of operation as well as on its high sensitivity and resolution. Some arrangements of essential necessity are involved therein, such as to avoid an errorneous wavelength readout for a noisy incidence of pulsed field. The ultimate accuracy of wavelength measurement is prescribed by the resolving power of the thickest etalon employed. As applied to the pulsed source, the model determines the wavelength to the accuracy of ±one part in 107 for even a single shot nanosecond incidence of a fraction of μJ energy. The design and performance are described in connection to pulsed dye-laser incidence.
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  • 83
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    Applied physics 31 (1983), S. 71-74 
    ISSN: 1432-0630
    Keywords: 42.60 ; 61.80 ; 78.50
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract By annealing Si layers amorphised by ion-implantation with a cw CO2 laser beam in oxygen rich environments, it has been possible to incorporate O atoms into the recrystallized lattice. Absorption measurements on such regrown layers by infrared spectrometry have shown that the impurities are preferentially bonded into substitutional lattice sites, rather than existing interstitially. Supplementary RHEED studies have indicated thatβ-cristobalite, a crystalline form of SiO2, has been formed. These investigations highlight the importance of the ambient during cw laser annealing.
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  • 84
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    Applied physics 24 (1981), S. 65-66 
    ISSN: 1432-0630
    Keywords: 42.72.+h ; 42.55.Fn ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A simple method for stabilizing a He-Xe laser, which simultaneously operates at two wavelengths of 3.51 μm and 3.37 μm, is described. This stabilization method depends on the comparison of the light intensities of the two wavelengths, and has a possibility of realizing a stability of better than 1×10−8 with unmodulated output.
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  • 85
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    Applied physics 21 (1980), S. 95-114 
    ISSN: 1432-0630
    Keywords: 42.55 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The development of excimers and excimer lasers are reviewed. The excimers of the noble gases (Xe 2 * , Kr 2 * , Ar 2 * ) and of the noble gas halides (e.g. KrF, XeCl) which, respectively, radiate in the vacuum ultra-violet and ultra-violet regions of the spectrum are described in terms of their structure, spectroscopy and formation kinetics and the methods of pumping, operational characteristics and applications of the lasers are discussed.
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  • 86
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    Applied physics 21 (1980), S. 215-219 
    ISSN: 1432-0630
    Keywords: 42.60
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Abstract The temporal coherence function |Γ(τ)| of picosecond pulses from a Nd: YAG laser has been measured by diffraction at a transient grating. The coherence timet c =7 ps is small compared to the pulse widtht p =22 ps but is in correspondence with the spectral bandwidth Δv=12·1010 Hz. The new method for measuring the coherence function |Γ(τ)| is discussed and compared with a previous experiment.
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  • 87
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    Applied physics 23 (1980), S. 253-258 
    ISSN: 1432-0630
    Keywords: 42.60 ; 78.30 ; 78.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The effect of solvents on the gain and the peaks of gain spectra is investigated for rhodamine 6G (R6G) under nitrogen laser pumping. The non-specific solute-solvent interaction for R6G is found to be mainly dispersive. Additional shifts produced for R6G in certain solvents are explained as due to hydrogen bonding. The gain characteristics are interpreted on the basis of the position and overlap of absorption and fluorescence spectra, the quantum yield, the absorption at the pumping wavelength and the refractive index of the solvent. For the solvents studied it is found that R6G has high gain in solvents with low refractive index and high polarity. Amongst the solvents studied the alcohols displayed the highest gain with methanol being the most suitable solvent. The lowest gain amongst the alcohols was with capryllic alcohol which gave a gain that was 69% of that obtained with methanol. Amongst all the solvents studied the lowest gain was with chloroform, being only 34% of the gain in methanol.
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  • 88
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    Applied physics 24 (1981), S. 261-265 
    ISSN: 1432-0630
    Keywords: 42.60
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    Notes: Abstract The present paper reports on the first observation of an anomalous Zeeman effect on the hyperfine components of theF 2 (2) line in methane (λ=3.39 μm), on an asymmetry of a nonlinear resonance in the magnetic field due to the influence of recoid, and on measurements of shifts of individual hyperfine components in weak magnetic fields.
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  • 89
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    Applied physics 32 (1983), S. 159-161 
    ISSN: 1432-0630
    Keywords: 66.30 ; 61.70 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The entire sodium ion content of sodiumβ″ alumina (Na1.67Mg0.67Al10.33O17) can be replaced with a variety of lanthanide ions by simple diffusion reactions at moderate temperatures (500–700°C). Lanthanideβ″ alumina crystals are hard, clear, chemically stable, and have well-defined crystal structures. The fluorescence spectrum of Nd3+ inβ″ alumina is similar to that in YAG. The lifetime of the4 F 3/2 state of Nd3+ in completely-exchangedβ″ alumina (350μs at 1021 Nd3+ cm−3) is about 45% longer than in YAG (240μs at 1020Nd3+ cm−3). The lanthanideβ″ aluminas may be of considerable interest as new phosphor and laser host materials.
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  • 90
    ISSN: 1432-0630
    Keywords: 42.60 ; 68.55 ; 61.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract New results are reported concerning the vanadium oxidation by cw CO2 laser irradiation in air at atmospheric pressure. Particular emphasis is paid both to the initial stage and the development of the oxidation process under the action of the laser radiation. Some aspects are finally discussed concerning the quantitative theoretical interpretation of the experimentally recorded data.
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  • 91
    ISSN: 1432-041X
    Keywords: Vitellin ; Yolk granule ; Yolk protein ; Silkworm ; Embryogenesis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Vitellin was purified from eggs of the silkworm,Bombyx mori, by a new method in which vitellin was extracted from isolated yolk granules. The purified vitellin had a molecular weight of 540,000. An antibody against purified vitellin was prepared in rabbits. It reacted with the hemolymph vitellogenin as well as with purified vitellin, but not with other proteins in the hemolymph or in the extract from yolk granules. The anti-vitellin IgG was used to immunocytochemically locate vitellin in theBombyx non-diapause egg during early developmental stages. In the egg, just after oviposition, vitellin was located in internal yolk granules and in small yolk granules of the periplasm. During the early developmental stages studied, vitellin was not metabolized uniformly throughout the egg. The vitellin of the internal yolk granules located at the posterior-dorsal part and of the small peripheral yolk granules was utilized in 16 h and 2 days, respectively, after oviposition. A thin, very vitellin-poor layer was located between the periplasm and the vitellin-rich interior in the newly laid egg. it was always in close contact with the periphery where blastoderm and germ-band cells developed.
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  • 92
    ISSN: 1432-041X
    Keywords: Major haemolymph proteins ; Development ; Cuticle ; Immunocytochemistry ; Ceratitis capitata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The developmental profile of the major haemolymph proteins (ceratitins) inCeratitis capitata was studied. Ceratitin concentration in the haemolymph decreases dramatically during the last days of pupal life, while the amounts of ceratitins in whole organism extracts remain unchanged. By electrophoretic, immunological and immunofluorescence techniques it was revealed that ceratitins are reabsorbed by the fat body and a fraction of them is deposited in the cuticle. The possible role of ceratitins is discussed.
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  • 93
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; Lectin (localization) ; Phaseolus (lectin) ; Phytohemagglutinin ; Seed (lectin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined the properties and subcellular localization of phytohemagglutinin (PHA), the major lectin of the common bean (Phaseolus vulgaris.), in the axis cells of nearly mature and imbibed mature seeds. On a protein basis the axis contained about 15% as much PHA as the cotyledons. Localization of PHA was done with an indirect immunolabeling method (rabbit antibodies against PHA, followed by colloidal gold particles coated with goat antibodies against rabbit immunoglobulins) on ultra-thin cryosections which were embedded in plastic on the grids after the immunolabeling procedure. The embedding greatly improved the visualization of the subcellular structures. The small (4 nm) collodial gold particles, localized with the electron microscope, were found exclusively over small vacuoles or protein bodies in all the cell types examined (cortical parenchyma cells, vascular-bundle cells, epidermal cells). The matrix of these vacuoles-protein bodies appears considerably less dense than that of the protein bodies in the cotyledons, but the results confirm that in all parts of the embryo PHA is localized in similar structures.
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  • 94
    ISSN: 1432-2048
    Keywords: Cotyledons ; Endoplasmic reticulum ; Ferritin labeling ; Immunocytochemistry ; Phaseolus ; Protein (reserve) ; Reserve protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ultrastructure of the storage parenchyma cells of the cotyledons of developing bean (Phaseolus vulgaris L.) seeds was examined in ultrathin frozen sections of specimens fixed in a mixture of glutaraldehyde, formaldehyde and acrolein, infused with 1 M sucrose, and sectioned at-80° C. Ultrastructural preservation was excellent and the various subcellular organelles could readily be identified in sections which had been stained with uranyl acetate and embedded in Carbowax and methylcellulose. The cells contained large protein bodies, numerous long endoplasmic reticulum cisternae, mitochondria, dictyosomes, and electron-dense vesicles ranging in size from 0.2 to 1.0 μm. Indirect immunolabelling using rabbit immunoglobulin G against purified phaseolin (7S reserve protein), and ferritin-conjugated goat immunoglobulin G against rabbit immunoglobulin G was used to localize phaseolin. With a concentration of 0.1 mg/ml of anti-phaseolin immunoglobin G, heavy labeling with ferritin particles was observed ober the protein bodies, the cisternae of the endoplasmic reticulum, and the vesicles. The same structures were lightly labeled when the concentration of the primary antigen was 0.02 mg/ml. Ferritin particles were also found over the Golgi bodies. The absence of ferritin particles from other organelles such as mitochondria and from areas of cytoplasm devoid of organelles indicated the specificity of the staining, especially at the lower concentration of anti-phaseolin immunoglobulin G.
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  • 95
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; (PEP carboxylase) ; PEP carboxylase ; Sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The localization of phosphoenol pyruvate carboxylase (EC 4.1.1.3.1.) in the leaf cells of Sorghum vulgare was investigated by using three techniques: the conventional aqueous and non aqueous methods gave conflicting results; the immunocytochemical techniques clearly showed that the enzyme is predominantly located in the cytoplasm of mesophyll cells.
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  • 96
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    Cell & tissue research 237 (1984), S. 195-202 
    ISSN: 1432-0878
    Keywords: Pituitary ; Prolactin cells ; Estrogen ; Heterogeneity ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This study was conducted to determine the functional and/or developmental relationships among three heterogeneous types of prolactin cells (I, II and III) in rats. Rats were injected subcutaneously daily with estradiol or testosterone propionate on days 10–20 after birth. Estradiol increased the proportion of cell types II and III, increased serum PRL levels 12-fold in males and 15-fold in females, and increased pituitary levels of prolactin 12-fold in males and 5-fold in females. Testosterone mainly increased the proportion of the Type-II cells, decreased serum levels of prolactin in males only, and did not change pituitary levels of prolactin. In a second experiment, treatment of rats with nafoxidine for five days after E2 treatment (days 10–20 after birth) increased the proportion of Type-I cells and decreased the proportion of Type-III cells and decreased serum and pituitary levels of prolactin by 50% in females and by 15 and 45% in males. In a third experiment utilizing adult male rats, estradiol and testosterone were found to modulate the relative ratios of the different types of PRL cells as they did in immature animals. The data taken as a whole suggest the possibility of an estrogen-stimulated conversion of one cell type to another, which may be a reflection of prolactin secretory activity.
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  • 97
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    Cell & tissue research 237 (1984), S. 245-252 
    ISSN: 1432-0878
    Keywords: Pineal organ ; Interstitial cells ; Astrocytes ; Immunocytochemistry ; Rat ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antigenic markers characteristic of astrocytes and their differentiative states (i.e., glial fibrillary acidic protein (GFAP), vimentin, and M1 and C1 antigens) were investigated in the pineal gland of mouse and rat using double immunolabeling techniques. In both species the socalled interstitial cells as characterized by TEM were shown to be astrocytes, since they expressed vimentin, but neither fibronectin (a marker for fibroblasts and endothelial cells) nor the neuron-specific L1 antigen or tetanus toxin receptors. Subpopulations of vimentin-positive pineal astrocytes were also GFAP- and C1- antigen-positive. M1- antigenpositive cells were not detected. It is concluded that a considerable proportion of interstitial cells in the pineal gland of rat and mouse are immature astrocytes which, in contrast to other parts of the central nervous system, persist into adulthood.
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  • 98
    ISSN: 1432-0878
    Keywords: Subcommissural organ ; Reissner's fiber ; Ependyma ; Secretory process ; Comparative analysis ; Immunocytochemistry ; Vertebrates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The subcommissural organs (SCO) of 76 specimens belonging to 25 vertebrate species (amphibians, reptiles, birds, mammals) were studied by use of the immunoperoxidase procedure. The primary antiserum was obtained by immunizing rabbits with bovine Reissner's fiber (RF) extracted in a medium containing EDTA, DTT and urea. Antiserum against an aqueous extract of RF was also produced. The presence of immunoreactive material in cell processes and endings was regarded as an indication of a possible route of passage. Special attention was paid to the relative development of the ventricular, leptomeningeal and vascular pathways established by immunoreactive structures. The SCO of submammalian species is characterized by (i) a conspicuous leptomeningeal connection established by ependymal cells, (ii) scarce or missing hypendymal cells, and (iii) a population of ependymal cells establishing close spatial contacts with blood vessels. The SCO of most mammalian species displays the following features: (i) ependymal cells lacking immunoreactive long basal processes, (ii) hypendymal secretory cells occurring either in a scattered arrangement or forming clusters, (iii) an occasional leptomeningeal connection provided by hypendymal cells, and (iv) in certain species numerous contacts of secretory cells with blood vessels. In the hedgehog immunoreactive material was missing in the ependymal formation of the SCO, but present in hypendymal cells and in the choroid plexuses. The SCO of several species of New-and Old-World monkeys displayed immunoreactive material, whereas that of anthropoid apes (chimpanzee, orangutan) and man was completely negative with the antisera used.
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  • 99
    ISSN: 1432-0878
    Keywords: Subcommissural organ ; Ependyma ; Comparative aspects ; Immunocytochemistry ; Secretory process ; Blood vessels
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In 76 specimens (amphibians, reptilians, mammals) belonging to 25 different vertebrate species, the region of the subcommissural organ (SCO) was investigated with the use of a primary antiserum raised against an extract of bovine Reissner's fiber+the immunoperoxidase procedure according to Sternberger et al. (1970). In the SCO of a toad (Bufo arenarum) and several species of reptiles (lacertilians, ophidians, crocodilians), the ependymal cells were the only type of secretory cell displaying vascular contacts, whereas in mammals ependymal and hypendymal cells established intimate spatial contacts with blood vessels. In Bufo arenarum, but especially in the reptilian species examined, the ependymo-vascular relationship was exerted by a population of ependymal cells having a rather constant location within the SCO and projecting to capillaries that showed a remarkably constant pattern of anatomical distribution. In the SCO of mammals the modality and degree of the structural relationships between secretory cells and blood vessels varied greatly from species to species. In the SCO of the armadillo and dog the secretory tissue was organized as a thick, highly vascularized layer with most of the cells oriented toward the capillaries. A rather opposite situation was found in the SCO of New-and Old-World monkeys, where vascular contacts were restricted to a few ependymal cells.
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  • 100
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    Cell & tissue research 237 (1984), S. 521-524 
    ISSN: 1432-0878
    Keywords: α-Melanocyte-stimulating hormone ; α-MSH-like peptide ; Immunocytochemistry ; Hypothalamus ; Lizard (Lacerta muralis)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of α-melanocyte-stimulating hormone (α-MSH) was studied in the brain of the lizard Lacerta muralis by means of immunocytochemical staining methods. α-MSH-like containing cells were found in the ventro-lateral preoptic area and the paraventricular and supraoptic nuclei. Some scattered cells staining for α-MSH were also detected in the mesencephalo-diencephalic boundary region, while numerous α-MSH-like nerve fibres were localized in the medial eminence. No reaction was observed after the use of antiserum preabsorbed with synthetic antigen. These findings suggest that an α-MSH-like peptidergic system could possibly be involved in the hypothalamo-hypophysial regulation and/or play a role as neurotransmitter in this animal.
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