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  • Adaptation
  • Climate change
  • GPS
  • Triticum aestivum
  • ddc:551
  • Springer  (66)
  • Springer Berlin Heidelberg
  • 2000-2004  (66)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Regional environmental change 1 (2000), S. 70-77 
    ISSN: 1436-378X
    Keywords: Key words Mountain vegetation ; Climate change ; Schynige Platte ; Switzerland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Climate change has in the past led to shifts in vegetation patterns; in a future, warmer climate due to enhanced greenhouse-gas concentrations, vegetation is also likely to be highly responsive to such warming. Mountain regions are considered to be particularly sensitive to such changes. In this paper we present an approach to assess the impact of climate change on long-term vegetation plots at the high-elevation site of the Schynige Platte, 2000 m above sea level, in the Bernese Alps (Switzerland). Records of vegetation spanning the period from 1928 to today at two different sites, each with several plots, were considered. The observed change in the species composition was then related to changes in land use and climate. We used daily values of temperature, snow and precipitation from several high-elevation weather stations to conduct these analyses. The correlation between climate and vegetation patterns revealed that species that prefer low thermal conditions move out of the plots, i.e., their frequency of occurrence is negatively correlated with the average number of degree-days over the last six decades. On the other hand, species with higher thermal demands are seen to be invading the plots, i.e., their frequency of occurrence is positively correlated to the average number of degree-days. Nutrient changes – though independent from climate – also play an important role in the observed shifts in species.
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  • 2
    ISSN: 1433-4909
    Keywords: Key words Olive wastes ; Bacillus ; Alkaliphile ; Growth characteristics ; Lipid composition ; Phylogeny ; Adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel Gram-positive facultatively alkaliphilic, sporulating, rod-shaped bacterium, designated as WW3-SN6, has been isolated from the alkaline washwaters derived from the preparation of edible olives. The bacterium is nonmotile, and flagella are not observed. It is oxidase positive and catalase negative. The facultative alkaliphile grows from pH 7.0 to 10.5, with a broad optimum from pH 8.0 to 9.0. It could grow in up to 15% (w/v) NaCl, and over the temperature range from 4° to 37°C, with an optimum between 27° and 32°C: therefore, it is both halotolerant and psychrotolerant. The bacterium is sensitive to a range of β-lactam, sulfonamide, and aminoglycoside antibiotics, but resistant to trimethoprim. The range of amino acids, sugars, and polyols utilized as growth substrates indicates that this alkaliphile is a heterotrophic bacterium. d(+)-glucose, d(+)-glucose-6-phosphate, d(+)-cellobiose, starch, or sucrose are the substrates best utilized. The major membrane lipids are phosphatidylglycerol and diphosphatidylglycerol, with smaller amounts of phosphatidylethanolamine and an unknown phospholipid. During growth at high pH, the proportion of phosphatidylglycerol is increased relative to phosphatidylethanolamine. The fatty acyl components in the membrane phospholipids are mainly branched chain, with 13-methyl tetradecanoic and 12-methyl tetradecanoic acids as the predominant components. The G + C content of the genomic DNA is 41.1 ± 1.0 mol%. The results of 16S ribosomal RNA sequence analysis place this alkaliphilic bacterium in a cluster, together with an unnamed alkaliphilic Bacillus species (98.2% similarity).
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  • 3
    Electronic Resource
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    Springer
    Nutrient cycling in agroecosystems 56 (2000), S. 59-68 
    ISSN: 1573-0867
    Keywords: bicarbonate-extractable potassium ; muriate of potash ; potassium ; potassium chloride ; relative effectiveness ; silicate rock powder ; Triticum aestivum ; Trifolium subterraneum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Granite (silicate) rock dust, a by-product of quarry operations, is being advocated and used as a fertilizer in the wheatbelt of south-western Australia (WA). The dust is insoluble and based on its nutrient element content (1.9% K and 0.3%P and negligible N) it is not expected to be a useful fertilizer. Previous laboratory studies and glasshouse experiments in WA suggest the dust is a slow release K fertilizer. This paper extends the previous studies to consider the dust as an NP or K fertilizer in the year of application in a field experiment on a soil deficient in N, P and K. In addition, the effectiveness of the dust as a K fertilizer was compared with the effectiveness of KCl (muriate of potash), the K fertilizer used in WA at present, in glasshouse experiments using K deficient soils. In the field experiment, compared with NP fertilizer or NPK fertilizer (urea, supplying N; superphosphate, providing P, S, Ca, Cu, Zn and Mo; KCl providing K), the dust had no effect on grain yield of wheat (Triticum aestivum); in fact dust applied at 20 t ha-1, for unknown reasons, reduced yields by about 65% compared to the nil (no fertilizer, no dust) treatment. Relative to the nil treatment, applying NPK fertilizer increased yields about threefold, from 0.54 to 1.79 t ha. The glasshouse experiments showed that, relative to KCl, the dust was from about 0.02 to 14% as effective in K deficient grey sandy soils for producing dried tops of 30-day old wheat plants or 42-day old clover (Trifolium subterraneum) plants. In soils with adequate K (yellow sands, sandy loams or clays, loamy clays, clay loams and clays), neither KCl nor the dust affected yields of 30 to 42-day old wheat or clover plants grown in the glasshouse. In the glasshouse experiments, no yield depressions were measured for the dust applied up to 17 g dust per kg soil (equivalent to 17 t dust ha-1 mixed into the top 10 cm of soil in the field). It is concluded that the dust has no value as a fertilizer.
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  • 4
    Electronic Resource
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    Springer
    Nutrient cycling in agroecosystems 56 (2000), S. 117-123 
    ISSN: 1573-0867
    Keywords: labelled nitrogen ; Lolium perenne ; nitrogen cycling ; root biomass ; straw ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Large amounts of nitrogen (N) fertiliser (150–200 kg N/ha) are currently being applied to perennial ryegrass (Lolium perenneL.) seed crops in New Zealand. Due to increasing requirements for efficient use of N fertilisers and minimising nitrate contamination of the environment, a field experiment was established using 15N-labelled fertiliser to follow the fate of applied N. Urea-15N was applied to a perennial ryegrass seed crop in April (30 kg N/ha), August (30 kg N/ha), September (60 kg N/ha) and October (60 kg N/ha). The urea-15N was applied in solution and watered in to minimise volatilisation loss. At the time of harvest (December), 9% of the applied 15N was in the seed, 29% in the straw, 19% in the roots and 39% in the soil organic matter. Losses of 15N were minimal as the N was applied in several applications, each one at a relatively low rate, and at times when leaching was unlikely to occur. Ryegrass plants used a greater proportion of the N applied in September and October (61–65%) compared with that applied in April (44%). Consequently more N was recovered from the soil in the autumn application (57%) than from the September and October applications (28–44%). The availability of the residual fertiliser N to a subsequent wheat (Triticum aestivum L.) crop was studied in a glasshouse experiment. The residual fertiliser N was present in the soil and ryegrass roots and stubble. The wheat plants only recovered 7–9% of this residual N. Most of the N taken up by the wheat came from the soil organic N pool. Overall, applying a total of 180 kg N/ha to the ryegrass appeared to have minimal direct impact on the environment. In the short term N not used by the ryegrass plants contributed to the soil organic N pool.
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  • 5
    ISSN: 1432-1254
    Keywords: Key words Flowering ; Phenology ; Climate change ; Temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Abstract  This paper examines the mean flowering times of 11 plant species in the British Isles over a 58-year period, and the flowering times of a further 13 (and leafing time of an additional 1) for a reduced period of 20 years. Timings were compared to Central England temperatures and all 25 phenological events were significantly related (P〈0.001 in all but 1 case) to temperature. These findings are discussed in relation to other published work. The conclusions drawn from this work are that timings of spring and summer species will get progressively earlier as the climate warms, but that the lower limit for a flowering date is probably best determined by examining species phenology at the southern limit of their distribution.
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  • 6
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    International journal of biometeorology 44 (2000), S. 60-66 
    ISSN: 1432-1254
    Keywords: Key words Phenology ; Trends ; Climate change ; Central Europe ; Urban climate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Abstract  In order to examine the impacts of both large-scale and small-scale climate changes (urban climate effect) on the development of plants, long-term observations of four spring phenophases from ten central European regions (Hamburg, Berlin, Cologne, Frankfurt, Munich, Prague, Vienna, Zurich, Basle and Chur) were analysed. The objective of this study was to identify and compare the differences in the starting dates of the pre-spring phenophases, the beginning of flowering of the snowdrop (Galanthus nivalis) and forsythia (Forsythia sp.), and of the full-spring phenophases, the beginning of flowering of the sweet cherry (Prunus avium) and apple (Malus domestica), in urban and rural areas. The results indicate that, despite regional differences, in nearly all cases the species studied flower earlier in urbanised areas than in the corresponding rural areas. The forcing in urban areas was about 4 days for the pre-spring phenophases and about 2 days for the full-spring phenophases. The analysis of trends for the period from 1951 to 1995 showed tendencies towards an earlier flowering in all regions, but only 22% were significant at the 5% level. The trends for the period from 1980 to 1995 were much stronger for all regions and phases: the pre-spring phenophases on average became earlier by 13.9 days/decade in the urban areas and 15.3 days/decade in the rural areas, while the full-spring phenophases were 6.7 days earlier/decade in the urban areas and 9.1 days/decade earlier in the rural areas. Thus rural areas showed a higher trend towards an earlier flowering than did urban areas for the period from 1980 to 1995. However, these trends, especially for the pre-spring phenophases, turned out to be extremely variable.
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  • 7
    ISSN: 1432-1939
    Keywords: Key words Intraspecific genetic variation ; Photosynthesis ; Frost hardiness ; Climate change ; Growing season
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Geographic patterns of intraspecific variations in traits related to photosynthesis and biomass were examined in two separate common garden experiments using seed collected from 26 Sitka alder (Alnus sinuata Rydb.) and 18 paper birch (Betula papyrifera Marsh.) populations from climatically diverse locations in British Columbia, Canada. Exchange rates of carbon dioxide and water vapour were measured on 2-year-old seedlings to determine the maximum net instantaneous photosynthetic rate, mesophyll conductance, stomatal conductance, and photosynthetic water use efficiency. Height, stem diameter, root and shoot dry mass and fall frost hardiness data were also obtained. Mean population maximum photosynthetic rate ranged from 10.35 to 14.57 µmol CO2 m–2 s–1 in Sitka alder and from 14.76 to 17.55 µmol CO2 m–2 s–1 in paper birch. Based on canonical correlation analyses, populations from locations with colder winters and shorter (but not necessarily cooler) summers had higher maximum photosynthetic rates implying the existence of an inverse relationship between leaf longevity and photosynthetic capacity. Significant canonical variates based on climatic variables derived for the seed collection sites explained 58% and 41% of variation in the rate of photosynthesis in Sitka alder and paper birch, respectively. Since growing season length is reflected in date of frost hardiness development, an intrinsic relationship was found between photosynthetic capacity and the level of fall frost hardiness. The correlation was particularly strong for paper birch (r=–0.77) and less strong for Sitka alder (r=–0.60). Mean population biomass accumulation decreased with increased climate coldness. These patterns may be consequential for evaluation of the impact of climate change and extension of the growing season on plant communities.
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  • 8
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    Oecologia 122 (2000), S. 11-19 
    ISSN: 1432-1939
    Keywords: Key words Acclimation ; Atmospheric carbon dioxide ; Climate change ; Photosynthesis ; Shade tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We compared the CO2- and light-dependence of photosynthesis of four tree species (Acer rubrum, Carya glabra, Cercis canadensis, Liquidambar styraciflua) growing in the understory of a loblolly pine plantation under ambient or ambient plus 200 µl l–1 CO2. Naturally-established saplings were fumigated with a free-air CO2 enrichment system. Light-saturated photosynthetic rates were 159–190% greater for Ce. canadensis saplings grown and measured under elevated CO2. This species had the greatest CO2 stimulation of photosynthesis. Photosynthetic rates were only 59% greater for A. rubrum saplings under CO2 enrichment and Ca. glabra and L. styraciflua had intermediate responses. Elevated CO2 stimulated light-saturated photosynthesis more than the apparent quantum yield. The maximum rate of carboxylation of ribulose-1,5-bisphosphate carboxylase, estimated from gas-exchange measurements, was not consistently affected by growth in elevated CO2. However, the maximum electron transport rate estimated from gas- exchange measurements and from chlorophyll fluorescence, when averaged across species and dates, was approximately 10% higher for saplings in elevated CO2. The proportionately greater stimulation of light-saturated photosynthesis than the apparent quantum yield and elevated rates of maximum electron transport suggests that saplings growing under elevated CO2 make more efficient use of sunflecks. The stimulation of light-saturated photosynthesis by CO2 did not appear to correlate with shade-tolerance ranking of the individual species. However, the species with the greatest enhancement of photosynthesis, Ce. canadensis and L. styraciflua, also invested the greatest proportion of soluble protein in Rubisco. Environmental and endogenous factors affecting N partitioning may partially explain interspecific variation in the photosynthetic response to elevated CO2.
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  • 9
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    Sciences of soils 5 (2000), S. 10-21 
    ISSN: 1432-9492
    Keywords: Soil temperature ; Triticum aestivum ; Stubble retention ; Nitrogen ; Early growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Early growth and development are often lower when wheat is sown into standing stubble. A study was conducted to determine whether this difference in early growth could be explained by the effects of stubble on soil temperature in the vicinity of the young plant. The roles of nitrogen nutrition and soil strength were also assessed. Three crops were monitored (1990–1992), with the wheat being sown into either standing wheat stubble after a no-till fallow (NT), or into no-tilled plots from which the stubble had been removed by burning (NB). Measurements were made of wheat growth and development, soil and plant N, soil temperature and penetration resistance. The site was on a black earth near Warialda in the northern wheatbelt of New South Wales, Australia. In 1992 wheat was also grown under simulated stubble to isolate the shading and soil temperature effects of stubble from other factors. A significant (P〈0.05) relationship was found between average soil temperature and above ground dry matter (DM) at 65 days after sowing (DAS) but not at 107 DAS. This relationship accounted for differences in DM production at 65 DAS between NT and NB treatments in 1991 and 1992, but not in 1990. In that year the lower DM production in NT plots was associated with poorer N nutrition, and possibly disease. Laboratory incubations indicate that immobilisation of N as stubble decomposed could have contributed to this. Burning stubble produced no immediate increase in soil N availability, so that it is unlikely that N contained in stubble contributed to the difference. Soil strength differences between treatments and phytotoxic effects are unlikely to have contributed to growth differences in this soil.
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  • 10
    ISSN: 1432-2242
    Keywords: Key words Glutenin ; Triticum aestivum ; Wheat storage proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Low-molecular-weight (LMW) glutenin subunits consist mainly of two domains, one at the N- terminus which contains repeats of short amino-acid motifs, and a non-repetitive one rich in cysteine, at the C- terminal region. In previous reports, polyacrylamide-gel electrophoresis has been used to show that large size variation exists among LMW and HMW glutenin subunits, and it has been suggested that deletions and insertions within the repetitive region are responsible for these variations in length. In this study, PCR-amplification of genomic DNA (Triticum aestivum variety Chinese Spring) was used to isolate three full-length LMW glutenin genes: LMWG-MB1, LMWG-MB2 and LMWG-MB3. The deduced amino-acid sequences show a high similarity between these ORFs, and with those of other LMW glutenin genes. Comparisons indicate that LMWG-MB1 has probably lost a 12-bp fragment through deletion and that LMWG-MB1 and LMWG-MB2 have an insertion of 81 bp within the repetitive domain. The current study has shown direct evidence that insertions and/or deletions provide a mechanistic explanation for the allelic variation, and the resultant evolution, of prolamin genes. Single-base substitutions at identical sites generate stop codons in both LMWG-MB2 and LMWG-MB3 indicating that these clones are pseudogenes.
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  • 11
    ISSN: 1432-2242
    Keywords: Key words Natural selection ; Microecological diversity ; Microsatellites ; Adaptation ; T. dicoccoides ; Wild emmer wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genetic diversity at 28 microsatellite loci was studied in a natural population of Triticum dicoccoides at the Ammiad microsite, north of the Sea of Galilee, Israel. This microsite was subdivided into four major habitats, North, Valley, Ridge and Karst, and further subdivided into nine subhabitats. The units thus defined showed strong and highly significant differentiation in ecological factors; in particular with respect to cover, proximity and height of rocks, and surface soil moisture after early rains. The results showed that allele distributions at microsatellite loci were nonrandom and associated with habitats. Significant genetic differentiation and variation in repeat number were found among subpopulations in the four major habitats and nine subhabitats. Habitat-specific and -unique alleles and linkage disequilibria were observed in the Karst subpopulation. The subpopulations dwelling in drier habitats and subhabitats showed higher genetic diversities at microsatellite loci. These results suggest that natural selection, presumably through aridity stress, acts upon microsatellite divergence predominantly on noncoding sequences, thereby contributing to differences in fitness.
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  • 12
    ISSN: 1432-2242
    Keywords: Key words Wheat ; High-molecular-weight glutenin ; AS-PCR ; Glu-A1 locus ; Null allele ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The present work reports new PCR markers that amplify the complete coding sequence of the specific alleles of the high molecular weight (HMW) glutenin genes. A set of AS-PCR molecular markers was designed which use primers from nucleotide sequences of the Glu-A1 and Glu-D1 genes, making use of the minor diffeences between the sequences of the x1, x2* of Glu-A1, and the x5 and y10 of Glu-D1. These primers were able to distinguish between x2* and the x1 or xNull of Glu-A1. Also x5 was distinguishable from x2, and y10 from y12. The primers amplified the complete coding regions and corresponded to the upstream and downstream flanking positions of Glu-A1 and Glu-D1. Primers designed to amplify the Glu-A1 gene amplified a single product when used with genomic DNA of common wheats and the xNull allele of this gene. This work also describes the cloning and characterisation of the nucleotide sequence of this allele. It possesses the same general structure as x2* and x1 (previously determined) and differs from these alleles in the extension of the coding sequence for a presumptive mature protein with only 384 residues. This is due to the presence of a stop codon (TAA) 1215-bp downstream from the start codon. A further stop codon (TAG), 2280-bp downstream from the starting codon is also found. The open reading frame of xNull and x1 alleles has the same size in bp. Both are larger than x2* which shows two small deletions. The reduced size of the presumptive mature protein encoded by xNull could explain the negative effect of this allele on grain quality.
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  • 13
    ISSN: 1432-2242
    Keywords: Keywords AFLPs ; Bulked segregant analysis ; Marker-assisted selection ; Microsatellites ; Powdery mildew resistance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Molecular markers were identified in common wheat for the Pm24 locus conferring resistance to different isolates of the powdery mildew pathogen, Erysiphe graminis DM f. sp. tritici (Em. Marchal). Bulked segregant analysis was used to identify amplified fragment length polymorphism (AFLP) markers and microsatellite markers linked to the gene Pm24 in an F2 progeny from the cross Chinese Spring (susceptible)× Chiyacao (resistant). Two AFLP markers XACA/CTA-407 and XACA/CCG-420, and three microsatellite markers Xgwm106, Xgwm337 and Xgwm458, were mapped in coupling phase to the Pm24 locus. The AFLP marker locus XACA/CTA-407 co-segregated with the Pm24 gene, and XACA/CCG-420 mapped 4.5 cM from this gene. Another AFLP marker locus XAAT/CCA-346 co- segregated in repulsion phase with the Pm24 locus. Pm24 was mapped close to the centromere on the short arm of chromosome 1D, contrary to the previously reported location on chromosome 6D. Pm24 segregated independently of gene Pm22, also located on chromosome 1D. An allele of microsatellite locus Xgwm337 located 2.4±1.2 cM from Pm24 was shown to be diagnostic and therefore potentially useful for pyramiding two or more genes for powdery mildew resistance in a single genotype.
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  • 14
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    Theoretical and applied genetics 100 (2000), S. 32-38 
    ISSN: 1432-2242
    Keywords: Key words Waxy (Wx) protein ; Triticum aestivum ; Amylose content ; Starch ; Rapid Visco-Analyzer ; Swelling power ; Noodle quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Waxy (Wx) protein is a granule-bound starch synthase (GBSS) responsible for amylose production in cereal endosperm. Eight isolines of wheat (Triticum aestivum L.) having different combinations of presence and absence of three Wx proteins, Wx-A1, -B1, and -D1, were produced in order to elucidate the effect of Wx protein deficiencies on the apparent amylose content and starch-pasting properties. An improved SDS gel electrophoresis showed that ’Bai Huo’ (a parental wheat) carried a variant Wx-B1 protein from an allele, Wx-B1e. Thus, wheat lines of types 1, 2, 4, and 6 examined in this study contained a variant Wx-B1 allele and not the standard allele, Wx-B1a. The results from 3 years of experiments using 176 lines derived from two cross-combinations showed that apparent amylose content increased the least in type 8 (waxy) having no Wx proteins and, in ascending order, increased in type 5 (only the Wx-A1 protein is present) 〈type 7 (Wx-D1) 〈type 6 (Wx-B1) 〈type 3 (Wx-A1 and -D1) 〈type 4 (Wx-A1 and -B1) 〈type 2 (Wx-B1 and -D1) 〈type 1 (three Wx proteins). However, Tukey’ s studentized range test did not detect significant differences in some cases. Densitometric analysis suggested that the amylose content was related to the amount of the Wx protein in the eight types. Parameters in the Rapid Visco-Analyzer test and swelling power were correlated to amylose content. Consequently, amylose content and pasting properties of starch were determined to be influenced the most by the lack of the Wx-B1 protein, followed by a lack of Wx-D1, and leastly by the Wx-A1 deficiency, which indicated the presence of differential effects of the three null alleles for the Wx protein.
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  • 15
    ISSN: 1432-2242
    Keywords: Key words DNA markers ; Genetic diversity ; Agroecological area ; Quality ; Adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  For studying genetic differentiation caused by selection for adaptation and end-use, the allele frequencies of 42 microsatellites (MS), representative of the three wheat genomes, were analysed in a total of 60 wheat cultivars. The cultivars originate from three agroecological areas (AEAs) – Germany, Austria and Hungary – and represent equal numbers of ’quality wheats’ and ’feed wheats’ for each country. For the 42 loci, 202 alleles were detected using PAGE and silver staining. The average number of alleles per locus was 4.8, including four monomorphic loci. For 16 loci, null alleles were detected. Cluster analysis clearly differentiated the varieties according to the three AEAs and, within each AEA, into quality wheats from feed wheats. Analysis of variance revealed highly significant differences of distance data between AEAs as well as between quality groups. The correlation between genetic distance (GD) and pedigree data (coefficient of diversity, COD) was r s=0.45. The results have proven the excellent resolving power of MS in varietal differentiation, which arises through breeding under specific environmental conditions, and for different end-use.
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  • 16
    ISSN: 1432-2242
    Keywords: Keywords Composite populations ; Triticum aestivum ; Blumeria (Erysiphe) graminis f. sp. tritici ; Residual resistance effects ; Quantitative resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The evolution of adult plant resistance towards powdery mildew (caused by Blumeria graminis f. sp. tritici) was investigated in 11 wheat populations cultivated for 10 years in a French network for dynamic management (DM) of wheat genetic resources. The aims of the study were to compare the evolution of resistance in sites submitted to different powdery mildew pressure and to investigate the implication of specific resistance gene action in adult plant resistance. For this, 7 of the 11 populations were characterized for their composition of specific resistance genes (results presented in a former paper). Even though no population differed significantly from the initial PA0 pool for mean adult plant resistance, divergence appeared among the final populations. The populations with the highest adult plant resistance level originated from sites where powdery mildew pressure is known to be high (Vervins, Le Rheu), whereas populations with the lowest adult plant resistance corresponded to areas with no, or very low, powdery mildew pressure (Toulouse, Montreuil-Bellay). A residual effect of defeated specific resistance genes was hypothesized, as lines accumulating at least two specific resistance genes appeared more resistant. Additional quantitative resistance seemed to be involved in adult plant resistance. DM lines appeared then as an interesting source of variability for resistance towards powdery mildew. Moreover, as these lines had been grown in mixed populations they may be appropriate as components of a composite cultivar.
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  • 17
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    Theoretical and applied genetics 100 (2000), S. 519-527 
    ISSN: 1432-2242
    Keywords: Key words Wheat ; Triticum aestivum ; Physical mapping ; Deletion lines ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Extended physical maps of chromosomes 6A, 6B and 6D of common wheat (Triticum aestivum L. em Thell., 2n=6x=42, AABBDD) were constructed with 107 DNA clones and 45 homoeologous group-6 deletion lines. Two-hundred and ten RFLP loci were mapped, including three orthologous loci with each of 34 clones, two orthologous loci with each of 31 clones, one locus with 40 clones, two paralogous loci with one clone, and four loci, including three orthologs and one paralog, with one clone. Fifty five, 74 and 81 loci were mapped in 6A, 6B and 6D, respectively. The linear orders of the mapped orthologous loci in 6A, 6B and 6D appear to be identical and 65 loci were placed on a group-6 consensus physical map. Comparison of the consensus physical map with eight linkage maps of homoeologous group-6 chromosomes from six Triticeaespecies disclosed that the linear orders of the loci on the maps are largely, if not entirely, conserved. The relative distributions of loci on the physical and linkage maps differ markedly, however. On most of the linkage maps, the loci are either distributed relatively evenly or clustered around the centromere. In contrast, approximately 90% of the loci on the three physical maps are located either in the distal one-half or the distal two-thirds of the six chromosome arms and most of the loci are clustered in two or three segments in each chromosome.
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  • 18
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    Journal of geodesy 74 (2000), S. 421-430 
    ISSN: 1432-1394
    Keywords: Key words: GLONASS ; GPS ; Ambiguity resolution ; Satellite geodesy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying
    Notes: Abstract.  When processing global navigation satellite system (GLONASS) carrier phases, the standard double-differencing (DD) procedure cannot cancel receiver clock terms in the DD phase measurement equations due to the multiple frequencies of the carrier phases. Consequently, a receiver clock parameter has to be set up in the measurement equations in addition to baseline components and DD ambiguities. The resulting normal matrix unfortunately becomes singular. Methods to deal with this problem have been proposed in the literature. However, these methods rely on the use of pseudo-ranges. As pseudo-ranges are contaminated by multi-path and hardware delays, biases in these pseudo-ranges are significant, which may result in unreliable ambiguity resolution. A new approach is addressed that is not sensitive to the biases in the pseudo-ranges. The proposed approach includes such steps as converting the carrier phases to their distances to cancel the receiver clock errors, and searching for the most likely single-differenced (SD) ambiguity. Based on the results from the theoretical investigation, a practical procedure for GLONASS ambiguity resolution is presented. The initial experimental results demonstrate that the proposed approach is useable in cases of GLONASS and combined global positioning system (GPS) and GLONASS positioning.
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  • 19
    ISSN: 1432-2242
    Keywords: Key words Composite populations ; Triticum aestivum ; Blumeria (Erysiphe) graminis f. sp. tritici ; Selection ; Drift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Dynamic management has been proposed as a complementary strategy to gene banks for the conservation of genetic resources. The evolution of frequencies of genes for specific resistance towards powdery mildew (caused by Blumeria graminis f. sp. tritici) in populations of a French network for dynamic management of bread wheat genetic resources was investigated after 10 years of multiplication without human selection. The objective was to determine whether specific resistance gene diversity was maintained in the populations and whether any changes could be attributed to selection due to pathogen pressure. Seven populations, originating from four of the network sites, were characterized and compared to the initial population for six specific resistance gene frequencies detected by nine Blumeria graminis f. sp. tritici isolates. Diversity decreased at the population level, but because of a strong differentiation between the populations, this diversity was maintained at the network level. The comparison of Fst parameters estimated on neutral markers (RFLP) and on resistance gene data revealed that in two of the populations specific resistance genes had been selected by pathogen pressure, whereas evolution in two other populations seemed to be the result of genetic drift. For the three last populations, conclusions were less clear, as one had probably experienced a strong bottleneck and the other two presented intermediate Fst values. A dynamic management network with sites contrasted for pathogen pressure, allowing genetic drift in some populations and selection in others, appeared, at least on the short term, to be a good tool for maintaining the diversity of genes for specific resistance to powdery mildew.
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  • 20
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    Personal and ubiquitous computing 4 (2000), S. 241-244 
    ISSN: 1617-4917
    Keywords: GPS ; Location sensing ; Pinger ; Shopping jacket ; Wearable computer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract As part of the Bristol Wearable Computing Initiative, we are exploring location-sensing systems suitable for use with wearable computing. In this paper we present our findings, and in particular a wearable application — the ‘Shopping Jacket’ — which relies on a minimal infrastructure to be effective. We use two positioning devices, ‘pingers’ and GPS. The pinger is used to signal the presence of a shop, and to indicate the type of shop and its website. The GPS is used to disambiguate which branch of a high street chain is being passed. The wearable uses this information to determine whether the wearer needs to be alerted that they are passing an interesting shop, or to direct the wearer around a shopping mall. The Shopping Jacket integrates a wearable CardPC, GPS and pinger receivers, a near-field radio link, hand-held display, GSM data telephone and a speech interface into a conventional sports blazer.
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  • 21
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    Artificial life and robotics 4 (2000), S. 31-36 
    ISSN: 1614-7456
    Keywords: Genetic algorithm ; Hopping robot ; Adaptation ; Pattern generator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract This paper describes an application of genetic algorithm to generate a jumping motion pattern for a hopping robot. A central pattern generator is used to generate the motion pattern. The tuning parameters of the central pattern generator are regarded as genes and adjusted by the genetic algorithm, so that the hopping robot can jump continuously to the reference height with the minimum force. To realize online tuning of the parameters, new genetic operations such as few individuals, quick estimation, instant selection, and intentional mutation are introduced. The experimental results demonstrate the effectiveness of the proposed scheme.
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  • 22
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    Russian journal of plant physiology 47 (2000), S. 734-739 
    ISSN: 1608-3407
    Keywords: Triticum aestivum ; bioelectric potentials ; frost resistance ; critical points
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Winter wheat (Triticum aestivum L.) seedlings of three cultivars differing in frost resistance were used to study cooling-induced changes in the bioelectric potential. Measurements were performed with nonfreezing graphite–glycerol electrodes in the regime of monitoring. Upon a gradual change in air temperature from 20 to –15°C at the rates of 20 and 2°C/h, the bioelectric potential underwent abrupt transitions at certain moments, indicating changes in the physiological condition of plants. The time required for the achievement of these critical states, as well as the survival of plants after thawing, depended both on the temperature and the cooling rate. Apparently, these characteristics were related to the dynamics of phase transitions of water. Cultivar-specific features were manifested in the different abilities of plants to maintain free water in a supercooled state. It is supposed that the critical points are related to the cold resistance of colloid systems and to the temperature lethal for plants.
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  • 23
    ISSN: 1573-5052
    Keywords: Artemisia ; Climate change ; Pentaphylloides ; Photosynthesis ; Rocky Mountains ; Subalpine ; Transpiration ; Water potential ; Water use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gas exchange and water relations responses to warming were compared for two shrub species, Artemisia tridentata spp. vaseyana (Asteraceae), a widely distributed evergreen species of the Great Basin and the western slope of the Rocky Mountains, and Pentaphylloides floribunda (Rosaceae), a deciduous shrub limited in distribution to moist, high-elevation meadows. Plants were exposed to an in situ infrared (IR) climate change manipulation at the Rocky Mountain Biological Laboratory, near Crested Butte, CO. Measurements of gas exchange and water relations were made on the two species in July and August, 1993 from plants growing in situ in infrared-heated and control plots. Carbon dioxide uptake, water loss, leaf temperature, water use efficiency, and water potential were compared to test the hypothesis that leaf and soil responses to IR will cause leaf level changes in photosynthesis. Photosynthetic CO2 uptake and water use efficiency increased for A. tridentata (2.9 vs. 1.9 μmol m−2 s−1 and 1.2 vs. 0.7 mmol C/mol H2O) in the heated plots compared to the controls, while water potential was significantly lower in the heated plots (−1.1 vs. −0.5 MPa). The heating treatment decreased rates of photosynthesis for P. floribunda, but not significantly so. For A. tridentata, the results are consistent with the community-level changes observed with heating. Taken together, the evidence suggests that global warming is likely to result in increasing dominance of A. tridentata in subalpine meadow habitat now dominated by forbs.
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  • 24
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    Annals of biomedical engineering 28 (2000), S. 318-330 
    ISSN: 1573-9686
    Keywords: Biphasic ; Poroelastic ; Cartilage ; Finite element analysis ; Cell mechanics ; Tissue growth ; Adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Experimental evidence suggests that cells are extremely sensitive to their mechanical environment and react directly to mechanical stimuli. At present, it is technically difficult to measure fluid pressure, stress, and strain in cells, and to determine the time-dependent deformation of chondrocytes. For this reason, there are no data in the published literature that show the dynamic behavior of chondrocytes in articular cartilage. Similarly, the dynamic chondrocyte mechanics have not been calculated using theoretical models that account for the influence of cell volumetric fraction on cartilage mechanical properties. In the present investigation, the location- and time-dependent stress-strain state and fluid pressure distribution in chondrocytes in unconfined compression tests were simulated numerically using a finite element method. The technique involved two basic steps: first, cartilage was approximated as a macroscopically homogenized material and the mechanical behavior of cartilage was obtained using the homogenized model; second, the solution of the time-dependent displacements and fluid pressure fields of the homogenized model was used as the time-dependent boundary conditions for a microscopic submodel to obtain average location- and time-dependent mechanical behavior of cells. Cells and extracellular matrix were assumed to be biphasic materials composed of a fluid phase and a hyperelastic solid phase. The hydraulic permeability was assumed to be deformation dependent and the analysis was performed using a finite deformation approach. Numerical tests were made using configurations similar to those of experiments described in the literature. Our simulations show that the mechanical response of chondrocytes to cartilage loading depends on time, fluid boundary conditions, and the locations of the cells within the specimen. The present results are the first to suggest that chondrocyte deformation in a stress-relaxation type test may exceed the imposed system deformation by a factor of 3–4, that chondrocyte deformations are highly dynamic and do not reach a steady state within about 20 min of steady compression (in an unconfined test), and that cell deformations are very much location dependent. © 2000 Biomedical Engineering Society. PAC00: 8719Rr, 8717Aa, 0270Dh
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    Plant and soil 226 (2000), S. 11-19 
    ISSN: 1573-5036
    Keywords: Chlorpropham (CIPC) ; microtubules ; nuclei ; recovery ; roots ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The present ultrastructural investigation on the effects of 50 μM chlorpropham (previously called CIPC) on growing roots of wheat (Triticum aestivum (L.) Thell cv. Vergina) was undertaken to clarify the mechanism of a carbamate herbicide action in plant cells, since the wide range of responses of plant cells to carbamate herbicides is based mainly on immunofluorescence studies. Cells of control roots contained abundant microtubules both in interphase and mitotic arrays. In chlorpropham-treated roots, however, no microtubules could be detected at all, neither in dividing nor in differentiating cells. Cycling cells became binucleate, polyploid or contained incomplete cell walls, the result of inhibition of cytokinesis. In long-term drug treatments (24 h or more) the affected cells entered a new cycle, which, however, did not progress beyond mid-metaphase. The nuclei of binucleate cells initiated prophase synchronously. Small vacuoles and Golgi vesicles were trapped within the nucleoplasm of the multilobed nuclei. In roots recovering from 8 h chlorpropham treatment, cells continued to exhibit polyploid nuclei, intranuclear vacuoles and incomplete walls. Microtubules reappeared but they were sparse and lacked a definite orientation. Preprophase cells did not form normal preprophase bands of microtubules, while mitotic cells occasionally contained microtubules bound to chromosomes and converged to minipoles. It is concluded that chlorpropham disorganized directly microtubules in addition to irreversibly affecting microtubule organizing centres, which failed to further support microtubule arrays.
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  • 26
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    Plant and soil 226 (2000), S. 275-285 
    ISSN: 1573-5036
    Keywords: arbuscular mycorrhiza ; Daucus carota ; Glomus mosseae ; Glomus intraradices ; monoxenic culture ; N uptake ; Triticum aestivum
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract New information on N uptake and transport of inorganic and organic N in arbuscular mycorrhizal fungi is reviewed here. Hyphae of the arbuscular mycorrhizal fungus Glomus mosseae (Nicol. and Gerd.) Gerd. and Trappe (BEG 107) were shown to transport N supplied as 15N-Gly to wheat plants after a 48 h labelling period in semi-hydroponic (Perlite), non-sterile, compartmentalised pot cultures. Of the 15N supplied to hyphae in pot cultures over 48 h, 0.2 and 6% was transported to plants supplied with insufficient N or sufficient N, respectively. The increased 15N uptake at the higher N supply was related to the higher hyphal length density at the higher N supply. These findings were supported by results from in vitro and monoxenic studies. Excised hyphae from four Glomus isolates (BEG 84, 107, 108 and 110) acquired N from both inorganic (15NH4 15NO3, 15NO3 − or 15NH4 +) and organic (15N-Gly and 15N-Glu, except in BEG 84 where amino acid uptake was not tested) sources in vitro during short-term experiments. Confirming these studies under sterile conditions where no bacterial mineralisation of organic N occurred, monoxenic cultures of Glomus intraradices Schenk and Smith were shown to transport N from organic sources (15N-Gly and 15N-Glu) to Ri T-DNA transformed, AM-colonised carrot roots in a long-term experiment. The higher N uptake (also from organic N) by isolates from nutrient poor sites (BEG 108 and 110) compared to that from a conventional agricultural field implied that ecotypic differences occur. Although the arbuscular mycorrhizal isolates used contributed to the acquisition of N from both inorganic and organic sources by the host plants/roots used, this was not enough to increase the N nutritional status of the mycorrhizal compared to non-mycorrhizal hosts.
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  • 27
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    Plant cell, tissue and organ culture 63 (2000), S. 35-40 
    ISSN: 1573-5044
    Keywords: anther culture ; EDTA ; ferrous ions ; ferric ions ; Hordeum vulgare ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A suitable form of iron supplement in the induction medium was found to be important for further development of induced pollen embryos in barley and wheat cultivars (genotypes), especially those providing few green plants viain vitro androgenesis. Genotypes able to regenerate many green plants were less susceptible to the lack of iron in induction medium. Although Fe-EDTA was found to be a suitable form of iron in the induction medium, androgenesis was also induced on media containing non-chelated iron (Fe2+ and Fe3+ ions). EDTA alone without iron inhibited the androgenic response even in the wheat cv. Florida, a model cultivar for androgenesis in wheat. In all barley cultivars under study including cv. Igri, a model cultivar for androgenesis in barley, EDTA alone caused an almost total suppression of androgenesis.
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  • 28
    ISSN: 1573-5060
    Keywords: Cheyenne ; polymorphism ; RAPD ; recombinant inbred chromosome line(RICL) RFLP ; STS ; SSR ; Triticum aestivum ; Wichita
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Previously chromosome 3A of wheat (Triticum aestivum L.) was reported to carry genes influencing yield, yield components, plant height, and anthesis date. The objective of current study was to survey various molecular marker systems for their ability to detect polymorphism between wheat cultivars Cheyenne(CNN) and Wichita (WI), particularly for chromosome3A. Seventy-seven `sequence tagged site' (STS), 10simple sequence repeat (SSR), 40 randomly amplified polymorphic DNA (RAPD) markers, and 52 restriction fragment length polymorphism (RFLP) probes for wheat homoeologous group 3 chromosomes, were investigated. Three (3.9%) STS-PCR primer sets amplified polymorphic fragments for the two cultivars, of which one was polymorphic for chromosome 3A. Sixty percent of SSR markers detected polymorphism between CNN and WI of which 50% were polymorphic for chromosome 3A. Twenty percent of RAPD markers detected polymorphism between CNN and WI in general, but none of these detected polymorphism for chromosome 3A. Of the fifty-two RFLP probes, 78.8% detected polymorphism between CNN and WI for group 3 chromosomes with one or more of seven restriction enzymes and 42% of the polymorphic fragements were for chromosome 3A. These high levels of RFLP and SSR polymorphisms between two related wheat cultivars could be used to map and tag genes influencing important agronomic traits. It may also be important to reconsider RFLP as the most suitable marker system at least for anchor maps of closely related wheat cultivars.
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  • 29
    ISSN: 1573-5060
    Keywords: complementary genes ; hybrid necrosis ; spring wheat ; Triticum aestivum ; winter wheat ; winter × springwheat hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The distribution and allelic expressivity of hybrid necrosis genes (Ne 1 and Ne 2) were studied in 21 winter (mostly exotic) and 43 spring type elite wheat genotypes, by crossing them with two known testers, C 306 (Ne 1-carrier) and HD 2380 (Ne 2-carrier).Ne 1 gene was present in one north-west Himalayan winter wheat landrace, Shoure Local, but absent in the other winter as well as spring wheats. Ne 2 gene was prevalent to a much lower extent in the exotic winter wheat germplasm (31.57%) as compared to the recently developed Indian and Mexican spring wheat semidwarfs (69.80%). This may suggest that breeders have tried to preclude hybrid necrosis by selecting for non-carrier genotypes in the development of exotic winter wheats in contrast to the situation in spring wheats. Based on the degree of expression of hybrid necrosis genes in the F1 hybrids, the carrier genotypes were characterized with respect to the allelic strength of the hybrid necrosis genes. The 27 non-carrier genotypes of the two ecotypes identified in the present study have a greater potential use in future hybridization programmes so as to overcome the problem of hybrid necrosis.
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  • 30
    ISSN: 1573-5060
    Keywords: allelic variation ; Chinese wheat ; glutenin subunit ; seed storage protein ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Variation in the electrophoretic banding patterns of high-molecular-weight (HMW) glutenin subunits of 274hexaploid wheat (Triticum aestivum) varieties from China was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and 27 different major HMW glutenin subunits were identified. Each variety contained three to five subunits and 29different glutenin subunit patterns were observed in274 Chinese hexaploid wheats. Seventeen alleles were identified based on the comparison of subunits mobility with that previously identified in a set of standard hexaploid wheats. The Chinese hexaploid wheats exhibited allelic variation in HMW glutenin subunit composition and the variation differed from that of Japanese and hexaploid wheats of other countries.
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  • 31
    ISSN: 1573-5060
    Keywords: bunt infection ; bunt resistance ; frost resistance ; Tilletia caries ; T. foetida ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to determine the effects of bunt inoculation on frost resistance and winter hardiness in lines containing resistance genes, the bunt [Tilletia foetida (Wallroth) Liro, T. caries (DC.) Tulasne] susceptibility of wheat lines containing bunt resistance genesBt1 to Bt10 and the effect of the year on the degree of infection were studied over six years from 1991 to 1997 in an artificial inoculation nursery. Uninoculated and artificially inoculated wheat plants were tested for frost resistance in the phytotron in 1995 and in the field in boxes in three years from 1994/95 to 1996/97. The line withBt10 was very resistant, lines with Bt5, Bt6, Bt8 and Bt9 were resistant, the line with Bt4 was moderately resistant, those with Bt2 and Bt3 were moderately susceptible, the line with Bt1 was susceptible and the line with Bt7 was very susceptible to the local bunt population in Hungary. Bunt incidence also varied over years. The frost resistance of the Bt lines was generally lower after bunt inoculation than that of uninoculated plants. The increased frost kill in inoculated plants was not correlated with the extent of varietal susceptibility to bunt. Some lines with resistance, namely those with Bt5 (1.6% infection), Bt8 (0.6%) and Bt10 (0.0%), suffered significantly greater frost kill in the young plant stage as the result of bunt inoculation. By contrast, the Bt7line had excellent frost resistance and winter hardiness but suffered the greatest extent of bunt infection, whereas the Bt6 line had good frost resistance and good bunt resistance.
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    Plant and soil 222 (2000), S. 25-34 
    ISSN: 1573-5036
    Keywords: deficiency ; genotypic differences ; iron ; nutrient efficiency ; phytosiderophore ; tolerance ; Triticum aestivum ; Triticum durum ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tolerance to Zn deficiency in wheat germplasm may be inversely related to uptake and transport of Fe to shoots. The present study examined eight bread (Triticum aestivum) and two durum (T. turgidum L. conv. durum) wheat genotypes for their capacity to take up and transport Fe when grown under either Fe or Zn deficiency. Bread wheat genotypes Aroona, Excalibur and Stilleto showed tolerance to Zn and Fe deficiency, while durum wheat genotypes are clearly less tolerant to either deficiency. Roots of bread wheats tolerant to Zn deficiency exuded more phytosiderophores than sensitive bread and durum genotypes. Greater amounts of phytosideophores were exuded by roots grown under Fe than Zn deficiency. A relatively poor relationship existed between phytosiderophore exudation or the Fe uptake rate and relative shoot growth under Fe deficiency. At advanced stages of Zn deficiency, genotypes tolerant to Zn deficiency (Aroona and Stilleto) had a greater rate of Fe uptake than other genotypes. Zinc deficiency depressed the rate of Fe transport to shoots in all genotypes in early stages, while advanced Zn deficiency had the opposite effect. Compared with Zn-sufficient plants, 17-day-old Zn-deficient plants of genotypes tolerant to Zn deficiency had a lower rate of Fe transport to shoots, while genotypes sensitive to Zn deficiency (Durati, Yallaroi) had the Fe transport rate increased by Zn deficiency. A proportion of total amount of Fe taken up that was transported to shoots increased with duration of either Fe or Zn deficiency. It is concluded that greater tolerance to Zn deficiency among wheat genotypes is associated with the increased exudation of phytosiderophores, an increased Fe uptake rate and decreased transport of Fe to shoots.
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  • 33
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    Euphytica 112 (2000), S. 23-31 
    ISSN: 1573-5060
    Keywords: A granules ; B granules ; quantitative analysis ; starch quality ; triploid endosperm ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two lines of hexaploid wheat were crossed and the basic generations of parent, F1, F2 and back-cross were sown in a controlled-environment chamber. FreshF1 and back-cross grains were generated, so the material could be handled either as the standard set of basic generations on a whole-plant basis, or as an extended set on an embryo or endosperm basis. The experiment was repeated. Mature grains were harvested and the starch particle size distribution was analysed in 3284 grains from 111 plants. Means and variances were partitioned into additive, dominance and interaction components. Grains from cross-pollinations had B-granule contents between parental values, rather than of the maternal parent, indicating an involvement of the grain genotype. Quantitative models based on endosperm genotype gave a better fit to the data than those based on embryo genotype. The difference in starch B-granule content between the parents was largely due to additive genes. Dominant genes were also indicated, with the first dose in the triploid endosperm having a large effect while the second dose had little or none. Non-allelic interactions were significant in the second experiment where the use of more types of backcross made them more detectable. There were also small and significant residual effects of the maternal plant in the first experiment, attributed to the vigour of the F1 mother plant and to the cytoplasm of Sunco. Narrow-sense heritability was low, between 0.05and 0.18 depending on the generation. Transgressive segregation was not found, suggesting that all alleles tending to increase the B-granule content were found in the Sunco parent and none in ME71. There was also no detectable heterosis in this character. The results show that breeding and selection for a low B-granule content should be possible but a further reduction will require new and complementary genes.
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  • 34
    ISSN: 1573-5060
    Keywords: disease resistance ; doubled haploid ; Fusarium headblight ; genetic analysis ; Fusarium graminearum ; recombinant inbred ; Triticum aestivum ; wheat scab
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic constitution of resistance to Fusarium head blight (FHB, scab) caused by Fusarium graminearum in the Chinese wheat cultivar Sumai 3 and the Japanese cultivar Saikai 165 was investigated using doubled haploid lines (DHLs) and recombinant inbred lines (RILs). Frequency distributions of DHLs derived from two F1 crosses, Sumai 3 (very resistant to resistant; VR-R) / Gamenya (very susceptible; VS) and Sumai 3 / Emblem (VS), fitted well to 1: 2: 1 (resistant: moderately resistant: susceptible) ratios for reaction to FHB in the field. It is suggested that the resistance of Sumai 3 is controlled by two major genes with additive effects. One of the resistance genes may be linked in repulsion to the dominant suppressor B1 for awnedness with recombination values 15.1 ± 3.3% in Sumai 3 /Gamenya and 21.4 ± 4.3% in Sumai 3 / Emblem. Saikai 165 is a Japanese resistant line derived from an F1 Sumai 3 / Asakaze-komugi (moderately resistant; MR). The data for RILs derived from the cross Emblem / Saikai 165, indicates that three resistance genes control the resistance of Saikai 165.
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  • 35
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    Euphytica 113 (2000), S. 219-225 
    ISSN: 1573-5060
    Keywords: deficiency ; genotypic variation ; iron ; nutrient efficiency ; phytosiderophore ; Triticum aestivum ; Triticum durum ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tolerance to Fe deficiency of wheat genotypes exhibiting differential tolerance to Zn deficiency is not known, even though the relationship between Fe nutrition and differential tolerance of wheat genotypes to Zn deficiency has been hypothesised frequently. In the present experiment, eight Triticum aestivum and two T. turigidum L. conv. durum cultivars were grown in nutrient solution deficient in either Znor Fe. Three indices of tolerance to nutrient deficiency were compared: relative [(-nutrient/+nutrient) × 100] shoot growth, shoot dry weight under nutrient deficiency and relative shoot/root dry weight ratio. Genotypes Aroona, Excalibur, Stilleto and Trident were classified as tolerant to both Zn and Fe deficiency, while durum wheats Durati and Yallaroi were sensitive to Zn deficiency and moderate to sensitive to Fe deficiency. Genotypes Excalibur, Stilleto and Trident come from the same breeding programme and have the common parent (line MEC3 =Sonora64//TZPP/YAQUI54) that could have been the donor of the genes for tolerance to Zn deficiency. When Fe-deficient, all wheat genotypes were severely chlorotic but kept producing shoot and root dry matter at a relatively high rate, making the relationship between the relative shoot growth and the relative leaf chlorophyll content poor. This is the first report of wheat genotypes exhibiting multiple tolerance to Zn and Fe deficiencies.
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  • 36
    ISSN: 1573-5060
    Keywords: bread wheat ; breeding and environmental effects ; gene frequency ; geographical distribution ; hybrid necrosis ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using the Information and Analytical System of Wheat Genetic Resources GRIS 3.2, the peculiarities of distribution of hybrid necrosis genes in bread wheat in different regions of the world were analyzed. Considerable variation in frequencies of the Ne1 and Ne2 genes in regions with different moisture and heat supply was revealed. A significant effect of breeding on frequency dynamics of different genotypes Ne1ne2, ne1Ne2 and ne1ne2 was confirmed.
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  • 37
    ISSN: 1573-5087
    Keywords: abscisic acid ; drought stress ; Triticum aestivum ; wheat germ agglutinin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Expression of wheat germ agglutinin (WGA) gene inthe developing embryos of wheat (Triticumaestivum L. cv. C-306) was studied in relation toabscisic acid (ABA) accumulation under water stressconditions. Imposition of water stress resulted inelevated ABA levels in the embryos at threedevelopmental stages (18, 24 and 30 DPA). On thecontrary, the effect of drought stress on WGAaccumulation was stage dependent with significantincrease in WGA content being observed at only 24 DPA. Our results suggest that apart from ABA, otherfactors which are temporally expressed, are alsoinvolved in regulation of WGA gene expression.
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    Genetic resources and crop evolution 47 (2000), S. 281-284 
    ISSN: 1573-5109
    Keywords: agronomic traits ; isozymes ; landrace ; Triticum aestivum ; variation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A sample of an Argentinean landrace of wheat showed considerable variation in most of the evaluated morphological and agronomic characters. However, analyses with high molecular glutenins and two isozyme systems, known to be highly polymorphic among current cultivars, revealed very little or no variation, respectively. The large difference in the observed variation between morphoagronomic and biochemical characters is discussed.
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    Journal of chemical ecology 26 (2000), S. 2141-2154 
    ISSN: 1573-1561
    Keywords: Allelopathy ; phenolic acids ; 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one ; DIMBOA ; GC-MS-MS ; wheat ; Triticum aestivum ; weed suppression ; annual ryegrass ; Lolium rigidum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Wheat allelopathy has potential for weed suppression. Allelochemicals were identified in wheat seedlings, and they were exuded from seedlings into agar growth medium. p-Hydroxybenzoic, trans-p-coumaric, cis-p-coumaric, syringic, vanillic, trans-ferulic, and cis-ferulic acids and 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) were identified in both the shoots and roots of 17-day-old wheat seedlings and their associated agar growth medium. Wheat accessions with previously identified allelopathic activity tended to contain higher levels of allelochemicals than poorly allelopathic ones. The allelopathic compounds present in the shoots generally also were identified in the roots and in the agar medium. Allelochemicals were distributed differentially in wheat, with roots normally containing higher levels of allelochemicals than the shoots. When the eight allelochemicals were grouped into benzoic acid and cinnamic acid derivatives, DIMBOA, total coumaric, and total ferulic acids, the amount of each group of allelochemicals was correlated between the roots and the shoots. Most of the allelochemicals identified in the shoots and roots could be exuded by the living roots of wheat seedling into the agar growth medium. However, the amounts of allelochemicals in the agar growth medium were not proportional to those in the roots. Results suggest that wheat plants may retain allelochemicals once synthesized. The presence of allelochemicals in the agar growth medium demonstrated that wheat seedlings were able to synthesize and to exude phytotoxic compounds through their root system that could inhibit the root growth of annual ryegrass.
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  • 40
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    Precision agriculture 2 (2000), S. 147-161 
    ISSN: 1573-1618
    Keywords: GPS ; mobile robot ; robot control ; chained systems ; guidance system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An accurate vehicle guidance system is required for some precision agriculture operations. It can be used for high speed direct seeding on a farm tractor. Such a system also ensures neither overlapping nor missing areas during the harvest, even when visibility is poor. But, automatic path following in the field is a difficult problem. Many vehicle guidance systems have been studied. Some use two dimensional (2D) information, while others are based upon 3D information. Most of the sensors use output information relative to their environment without absolute reference of the path. As the new reference is based on the previous pass, one of the main problems encountered with the edge following systems is an increase in the amplitude of oscillations due to guidance errors in successive passes. Thus, human operators frequently and periodically sacrifice efficiency in one pass to “straighten out” the edge of the worked area. Another kind of sensor, centimeter accuracy Real-Time Kinematic based Global Positioning System (GPS) receivers, can be used. The advent of accurate systems, with a standard deviation from the mean of about 10 mm, allows the design and implementation of absolute vehicle guidance systems. A few approaches to GPS-based control systems also include attitude measurement sensors such as fiber optic gyroscope. The preliminary work presented in this paper was aimed at validating the use of a GPS receiver in a vehicle guidance system, without any orientation sensor. We have designed a controller to perform a line-following task. Real-time experiments have been carried out on a combine harvester.
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  • 41
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    Precision agriculture 2 (2000), S. 179-188 
    ISSN: 1573-1618
    Keywords: soil sampling ; sampler design ; quality control ; standardisation ; GPS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The quality of soil sampling has a significant effect on the accuracy of site specific fertiliser application. Soil sampling depth, number of cores and their distribution within a field or field section are important criteria for achieving a high quality sample. Considering the increased number of soil samples required for site specific fertiliser application, the chosen method of sampling needs to be fast, cost effective and accurate to provide reliable information. Soil sampling systems currently in use do not allow an automatic control of the precise soil sampling depth and the number of cores taken. The system described here uses a newly designed hydraulic auger, that samples the soil at a constant depth under varying field and soil conditions. This has been achieved by a specific shape and angle of the auger turns. The number of cores taken and their depth are controlled automatically by a sensor linked to an on-board computer. Furthermore, diagnostic tools identify errors and prevent any malfunction. The soil sampling system allows a fully automated and standardised sampling procedure, providing the required reliability for site specific fertiliser application.
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  • 42
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    Precision agriculture 2 (2000), S. 189-200 
    ISSN: 1573-1618
    Keywords: digital elevation model ; GPS ; topographic maps
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The digital elevation model (DEM) is considered by many to be an important base map for a precision farming GIS (Geographic Information System). Previous work has shown that dual-frequency survey grade GPS (Global Positioning System) receivers are capable of rapidly producing accurate positional data from a moving vehicle from which DEM's can be developed. However, this type of GPS receiver is expensive and somewhat difficult to use properly. This paper presents the results of a study to evaluate the potential of using single frequency sub-meter and 2–5 meter horizontal accuracy GPS receivers to enable the farmer to collect multiple passes of GPS data during normal field operations from which DEM's can be developed. The results show that when using a single frequency sub-meter GPS receiver: (1) it is possible to develop a DEM with standard deviation of the elevation accuracy on the order of 0.12–0.14 m, (2) in order to collect data with this level of elevation accuracy, data should only be collected when the GDOP (Geometric Dilution Of Precision) is less than 5.0, (3) at least 10 passes of data with appropriate data averaging is required to produce this level of elevation accuracy. The results also indicate that the vertical error associated with a 2–5 meter horizontal accuracy GPS receiver is such that it is not recommended for use in collecting data to develop DEM's in this application.
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  • 43
    ISSN: 1573-157X
    Keywords: base line ; earthquake risk area ; leveling ; fault deformation ; GPS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract In Capital area of China, many faultdeformational surveying sites were set up sequentiallyfrom 1967. With short leveling, short base line andshort ranging net across fault, a lot of data havebeen accumulated. In 1992, GPS (Global PositioningSystem) measurements were started in China. Two timesof GPS measurements have been made in 1992 and 1995respectively. The International Terrestrial ReferenceFrame 93 and the Precision Ephemeris provided byInternational GPS Geodynamics Service (IGS) fromGlobal GPS tracing network solution are used. In GPSanalysis and data processing, GAMIT software and GLOBKsoftware are used. The results from these two kinds ofdata are discussed. For earthquake risk area judgment,it is of interest to know where is compressional areaand where is tensional area, and generally thecompressional areas are considered as risk area andcalled abnormal area. All the data from shortleveling, short ranging (measured bylaser-range-finder), short base line (measured by baseline tape) and GPS methods can give us the descriptionabout the deformational state of the crust –compressional state or tensional state. The comparisonshows that, some of the two kinds of data areconsistent, a few of them are contradictory, and thenthe results are discussed.
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  • 44
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    Precision agriculture 2 (2000), S. 281-291 
    ISSN: 1573-1618
    Keywords: automatic steering ; GPS ; swath guidance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Precision agriculture requires automatic control of agricultural machinery in order to cope with the large amount of information which has to be applied during site specific field work. The driver is occupied with operating and supervising the controlled and non-controlled implements as well as with vehicle guidance. Steering, in particular, is monotonous and tiring. This can result in reduced operation quality. This was the motivation to develop a concept of a guidance system for agricultural vehicles. A self-propelled forage harvester was the test vehicle for this research. The harvester was equipped with an automatic steering system. Real Time Kinematic Global Positioning System (RTK-GPS) was used as the only positioning sensor due to high absolute accuracy within the field and its high reliability. The system performance was investigated under various test conditions. Additionally a guidance-path-planning for swath harvesting operations was developed. Both the automatically steered forage harvester and the path planner could be examined under real field conditions. The standard deviation of the driving performance was better than 100 mm under all conditions. The swaths could be collected reliably with the automatically steered forage harvester.
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    Nutrient cycling in agroecosystems 56 (2000), S. 109-116 
    ISSN: 1573-0867
    Keywords: labelled nitrogen ; Lolium perenne ; nitrogen cycling ; root biomass ; straw ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field trial was carried out to compare the transformations and plant uptake of urine N and S in a short-term pasture from within an arable/pasture ley rotation and a long-term pasture. Animal urine labelled with 15N and 35S was applied to microplots at both sites. These microplots were destructively sampled at various time intervals over 12 months and analysed for 15N and 35S. It is known that soil organic matter accumulates under short-term pastures compared with a long-term pasture in which accumulation and degradation are in balance. Consequently, it was hypothesised that immobilization of urine N and S is more intense in the short-term. However, in this study there was considerably less immobilization of 15N and 35S into soil organic forms under short-term pasture than long-term pasture. This was attributable to a greater pasture dry matter response to urine application under the short-term pasture (due to its inherently low N fertility) resulting in a greater plant uptake of 15N and 35S with less 15N and 35S consequently being available for immobilization. At both sites, all of the applied 35S was accounted for through plant uptake and recovery in the soil, but 21–48% of the 15N was unaccounted for and presumed to have been lost through gaseous emissions. It was concluded that accumulation of soil organic N and S under short-term pastures is likely to be attributable to turnover of plant residues (particularly root material) and does not appear to be related to immobilization in urine patches.
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    Plant cell, tissue and organ culture 60 (2000), S. 69-73 
    ISSN: 1573-5044
    Keywords: cereals ; tissue culture ; Triticum durum ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fertile, green plants were regenerated from immature inflorescence explants from each of four Canadian wheat cultivars. The cultivars were representative of four classes of Canadian wheat. Explants from immature inflorescences of three size ranges were cultured on two types of media: MSI/MSR, which contains 1650 mg l-1 NH4NO3and sucrose as a carbon source, and BII/BIR, which contains 250 mg l-1 NH4NO3and maltose as a carbon source. Regeneration from all cultivars was significantly better on BII/BIR media than on MSI/MSR media. On BII/BIR media, `AC Karma', `Plenty', and `Fielder' gave the highest number of shoots per 10 explants, where the explants were derived from immature inflorescences 5.1 to 10.0 mm in length. 'Columbus' did not regenerate on MSI/MSR medium, and regenerated poorly on BII/BIR medium. Differences were found between cultivars with regard to the number of regenerant plants produced with the best treatments: `Plenty' produced 16.1 shoots per 10 explants, `AC Karma' 12.4, `Fielder' 6.4, and `Columbus' 2.2.
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  • 47
    ISSN: 1573-5060
    Keywords: biochemical marker ; gene location ; Lophopyrum elongatum ; Puccinia striiformis ; stripe rust ; substitution line ; Triticum aestivum ; yellow rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A set of T. aestivum-L. elongatum chromosome substitution lines was tested for yellow rust resistance at the seedling stage. Inheritance of the resistance and esterase-5 (Est-5) variation were studied. The results demonstrated that L.elongatum carried a new gene(s) conferring yellow rust resistance. This gene was dominant and located on chromosome 3E of L. elongatum. The biochemical locus encoding Est-5was also located on chromosome 3E, and co-segregated with theYr gene(s) in the wheat background. The transmission frequencies of chromosome 3E in 3E(3A) × CS, 3E(3B) × CS and 3E(3D) × CS hybrids were scored.None of the hybrids transmitted the alien chromosome at thetheoretical maximum rate, but the transmission frequencies ofchromosome 3E in F2 populations of 3E(3A) × CS and 3E(3D) × CS were significantly higher than in thatof 3E(3B) × CS.
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  • 48
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    Euphytica 111 (2000), S. 199-203 
    ISSN: 1573-5060
    Keywords: Diuraphis noxia (Mordvilko) ; protein ; Russian wheat aphid ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko), has become a perennial, serious pest of wheat (Triticum aestivum L.) in the western United States. Current methodologies used to enhance RWA resistance in wheat germplasm could benefit from an understanding of the biochemical mechanisms underlying resistance to RWA. This study was initiated to identify specific polypeptides induced by RWA feeding that may be associated with RWA resistance. The effects of RWA feeding on PI 140207 (a RWA-resistant spring wheat) and Pavon (a RWA-susceptible spring wheat) were examined by visualizing, silver-stained denatured leaf proteins separated by two-dimensional polyacrylamide gel electrophoresis. Comparisons of protein profiles of noninfested and RWA-infested Pavon and PI 140207 revealed a 24-kilodalton-protein complex selectively inhibited in Pavon that persisted in PI 140207during RWA attack. No other significant qualitative or quantitative differences were detected in RWA-induced alterations of protein profiles. These results suggest that RWA feeding selectively inhibit synthesis and accumulation of proteins necessary for normal metabolic functions in susceptible plants.
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  • 49
    ISSN: 1573-5036
    Keywords: plant uptake ; potassium ; potassium starvation ; radiocaesium ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Short term experiments were carried out to investigate the effect of internal tissue potassium concentration on the uptake of radiocaesium by spring wheat (Triticum aestivum cv. Tonic). The results showed that potassium starvation increased Cs influx rates by a factor of 10 compared with non-starved plants. Solution to plant tissue transfer factor (TF) values also increased by around an order of magnitude after potassium starvation treatment. The enhancement of Cs influx rates by potassium starvation could be offset by an increase in external potassium concentration: this effect is minimal at external potassium concentrations greater than approximately 200 μM (8 mg L-1). This reveals that Cs influx into plant roots is subject to control by both internal and external potassium status. The kinetics of Cs uptake by potassium in starved and non-starved plants can be described adequately by the Michaelis-Menten equation. It was shown that potassium starvation substantially reduces the Km value from approximately 28 to 6 μM, which suggests that starvation treatment increases significantly the affinity of plant roots for Cs+. Mechanisms involved in K-Cs interactions during plant uptake are discussed in this paper. Finally, the relevance of such mechanisms as determinants of radiocaesium uptake by plants growing under different ecological conditions is emphasised.
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  • 50
    ISSN: 1573-5060
    Keywords: genetic diversity ; germplasm ; RFLP ; Triticum aestivum ; wheat breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A set of 292 accessions of common wheat (Triticum aestivum L.) representing 21 germplasm pools based on geographical or breeding program origins was assayed for RFLP diversity. Thirty cDNA and genomic DNA probes and the HindIII restriction enzyme were employed for RFLP analysis. About 61% of all 233 scored bands were present in 75% or more of the accessions. All but one of the 30 probes revealed polymorphism, and the average number of distinct patterns per probe over all accessions was 9.5.Polymorphic Information Content (PIC) values within a pool varied from 0 to 0.9 and depended on the identities of both the germplasm pool and the probe. Rare banding patterns with a relative frequency of ≤0.2 within a pool were detected. These rare patterns were more likely to occur in pools exhibiting high levels of heterogeneity. The highest level of polymorphism was observed in the Turkish landraces from Southwest Asia. The Eastern U.S. soft red winter wheat germplasm pool was more genetically diverse than the other advanced germplasm pools, and nearly as diverse as the Turkish landrace pool. RFLP-based genetic relationships between germplasm pools generally tracked expectations based on common geographical origin, breeding history and/or shared parentages. The Chinese wheat landraces from Sichuan, Tibet, and Yunnan provinces were distinct from other pools. Similarity matrices for among-pool genetic distance estimates based on either band frequencies or banding pattern frequencies showed good correlation with matrices derived from Nei and Li's mean genetic similarity estimates (r=−0.82** and r=−0.73**, respectively.
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  • 51
    ISSN: 1573-5079
    Keywords: canopy architecture ; canopy photosynthesis ; CO2 enrichment ; global change ; leaf area index ; leaf tip angle ; nitrogen stress ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response of whole-canopy net CO2 exchange rate (CER) and canopy architecture to CO2 enrichment and N stress during 1996 and 1997 for open-field-grown wheat ecosystem (Triticum aestivum L. cv. Yecora Rojo) are described. Every Control (C) and FACE (F) CO2 treatment (defined as ambient and ambient +200 μmol mol−1, respectively) contained a Low- and High-N treatment. Low-N treatments constituted initial soil content amended with supplemental nitrogen applied at a rate of 70 kg N ha−1 (1996) and 15 kg N ha−1 (1997), whereas High-N treatments were supplemented with 350 kg N ha−1 (1996 and 1997). Elevated CO2 enhanced season-long carbon accumulation by 8% and 16% under Low-N and High-N, respectively. N-stress reduced season-long carbon accumulation 14% under ambient CO2, but by as much as 22% under CO2 enrichment. Averaging both years, green plant area index (GPAI) peaked approximately 76 days after planting at 7.13 for FH, 6.00 for CH, 3.89 for FL, and 3.89 for CL treatments. Leaf tip angle distribution (LTA) indicated that Low-N canopies were more erectophile than those of High-N canopies: 48° for FH, 52° for CH, and 58° for both FL and CL treatments. Temporal trends in canopy greenness indicated a decrease in leaf chlorophyll content from the flag to flag-2 leaves of 25% for FH, 28% for CH, 17% for CL, and 33% for FL during 1997. These results indicate that significant modifications of canopy architecture occurs in response to both CO2 and N-stress. Optimization of canopy architecture may serve as a mechanism to diminish CO2 and N-stress effects on CER.
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    Chromosome research 8 (2000), S. 671-676 
    ISSN: 1573-6849
    Keywords: meiotic metaphase I pairing ; recombinant isochromosome ; Triticum aestivum ; Triticum dicoccoides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A recombinant isochromosome i5BLrec of wheat was developed with one arm and the proximal 36% of the other arm of Chinese Spring (CS) origin and the distal 64% of the recombined arm of Triticum turgidum subsp. dicoccoides origin. The i5BLrec} provides an unusual opportunity to analyze the role of the centromere or arm heterozygosity in chromosome prealignment and synapsis during meiosis. In monosomic condition, the i5BLrec formed a ring univalent in 86.8% of the pollen mother cells (PMCs) at meiotic metaphase I. In the disomic condition, the two i5BLrec preferentially paired as a normal bivalent in 74.8% of the PMCs, which differed significantly (p〈0.01) from the normal bivalent pairing of 51% observed in diisosomic 5BL chromosomes of the CS (Di5BLCS) control plants. In plants with one i5BLrec and a normal 5BCS, the long arm of 5BCS paired with the homologous arm of i5BLrec in 54.4% of the PMCs, and 40.4% of the PMCs had a 5BCS univalent and a i5BLrec ring univalent. The implications of the i5BLrec pairing data on the mechanism of Ph1 gene action are discussed.
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    Chromosome research 8 (2000), S. 501-511 
    ISSN: 1573-6849
    Keywords: BFB cycle ; chromosome healing ; gametocidal factor ; rye deficiencies ; Secale cereale ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gametocidal factor on the Aegilops cylindrical chromosome 2Cc was used to induce and analyze the nature of chromosomal rearrangements in rye chromosomes added to wheat. For this purpose we isolated plants disomic for a given rye chromosome and monosomic for 2Cc and analyzed their progenies cytologically. Rearranged rye chromosomes were identified in 7% of the progenies and consisted of rye deficiencies (4.6%), wheat–rye dicentric and rye ring chromosomes (1.8%), and terminal translocations (0.6%). The dicentric and ring chromosomes initiated breakage–fusion–bridge cycles (BFB) that ceased within a few weeks after germination as the result of chromosome healing. Of 56 rye deficiencies identified, after backcrossing and selfing, only 33 were recovered in either homozygous or heterozygous condition covering all rye chromosomes except 7R. The low recovery rate is probably caused by the presence of multiple rearrangements induced in the wheat genome that resulted in poor plant vigor and seed set, low transmission, and an underestimation of the frequency of wheat–rye dicentric chromosomes. Genomic in-situ hybridization (GISH) analysis of the 33 recovered rye deficiencies revealed that 30 resulted from a single break in one chromosome arm followed by the loss of the segment distal to the breakpoint. Only three had a wheat segment attached distal to the breakpoint. Although some of the Gc-induced rye rearrangements were derived from BFB cycles, all of the recovered rye rearrangements were simple in structure. The healing of the broken chromosome ends was achieved either by the de-novo addition of telomeric repeats leading to deficiencies and telocentric chromosomes or by the fusion with other broken ends in the form of stable monocentric terminal translocation chromosomes.
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  • 54
    ISSN: 1573-9058
    Keywords: chlorophyll fluorescence ; diphenyl carbazide ; donor side ; electron transport ; MnCl2 ; NH2OH ; photosystem ; senescence ; thylakoid protein ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in various components of photosynthetic apparatus during the 6-d dark incubation at 25 °C of detached control and DCMU-treated Triticum aestivum L. leaves were examined. The rate of photosystem 2 (PS2) activity was decreased with increase of the time of dark incubation in control leaves. In contrast to this, DCMU-treated leaves demonstrated high stability by slowing down the inactivation processes. Diphenyl carbazide and NH2OH restored the PS2 activity more in control leaves than in DCMU-treated leaves. Mn2+ failed to restore the PS2 activity in both control and DCMU-treated samples. Similar results were obtained when Fv/Fm was evaluated by chlorophyll fluorescence measurements. The marked loss of PS2 activity in dark incubated control leaves was primarily due to the loss of D1, 33, and 23 kDa extrinsic polypeptides and 28-25 kDa LHCP2 polypeptides.
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    Photosynthetica 37 (2000), S. 519-527 
    ISSN: 1573-9058
    Keywords: alanine ; aspartate ; glycine ; glycollate ; malate ; nitrate ; serine ; sugars ; Triticum aestivum ; urea ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 14CO2 uptake in leaves of wheat plants (Triticum aestivum L.) fertilized by urea or Ca(NO3)2 (25 mol m-3) was investigated. The Warburg effect (inhibition of 14CO2 uptake by oxygen) under 0.03 vol. % CO2 concentration was observed only in non-fertilized plants. Under 0.03 vol. % CO2, the Warburg antieffect (stimulation of 14CO2 uptake by oxygen) was detected only in plants fertilized by Ca(NO3)2. Under saturating CO2 concentration (0.30 vol. %), the Warburg antieffect was observed in all variants. Under limitation of ribulose-1,5-bisphosphate carboxylase/oxygenase activity (0.30 vol. % CO2 + 1 vol. % O2), the rate of synthesis of glycollate metabolism products decreased in control and urea-fertilized plants but was enhanced in nitrate-fed plants. Hence, there was an activation of glycollate formation via transketolase reaction in fertilized plants, and the products of nitrate reduction function were oxidants in nitrate-fertilized plants whereas the superoxide radical played this role in urea-fertilized plants.
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  • 56
    ISSN: 1573-9058
    Keywords: net photosynthetic rate ; soil and atmospheric drought ; stomatal conductance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Yields of wheat in semiarid and arid zones are limited by drought, and water condition is very important at each stage of development. Studies carried out at Loess Plateau in the northwestern part of China indicated that yield of spring wheat (Triticum aestivum L.) cv. Dingxi 81-392 was reduced by 41% when subjected to water stress. The effects of two water regimens on net photosynthetic rate (P N), stomatal conductance (g s), and intercellular CO2 concentration (C i) were investigated at the jointing, booting, anthesis, and grain filling stages. Low soil moisture in comparison to adequate one had invariably reduced P N during the diurnal variations at the four growth stages. P N and g s in both soil moisture regimes was maximally reduced at midday. C i and the stomatal limitation fluctuated remarkably during photosynthesis midday depression processes, especially at the grain filling stage. Hence atmospheric drought at midday was one of the direct causes inducing stomata closure and the g s depression, but it was beneficial for maintaining stable intrinsic water use efficiency. Fluctuation in C i implicated that non-stomatal limitation also plays an important role during the period of photosynthesis midday depression. Consequently stomatal and/or non-stomatal limitation are the possible cause of the midday photosynthesis decline.
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    Photosynthetica 37 (2000), S. 615-619 
    ISSN: 1573-9058
    Keywords: carbon based secondary compounds (CBSC) ; isoorientin ; leaf age ; litter ; phenolics ; tricin ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We compared flavonoids in green, mature, and senescing flag leaves of wheat grown under ambient (AC - 370 μmol mol-1) and elevated (EC - 550 μmol mol-1) concentrations of CO2 in a FACE (Free Air CO2 Enrichment) system. The concentrations of flag leaf flavonoids (e.g., isoorientin and tricin) decreased to one third in mature leaves, and the majoritary isoorientin almost disappeared in senescing leaves. Flavonoid concentrations increased in green well-developed flag leaves under EC (46 % isoorientin and 55 % tricin), whereas the differences disappeared in mature and senescing flag leaves. Predictions of changes in litter phenolic concentrations and their effects on decomposition rates under EC based on changes in green leaves need to be revised.
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    European journal of plant pathology 106 (2000), S. 837-842 
    ISSN: 1573-8469
    Keywords: Triticum aestivum ; Septoria nodorum ; Phaeosphaeria nodorum ; epidemiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The transmission of Stagonospora nodorum from four naturally infected winter wheat seedlots was quantified in controlled environment germination chambers at 9, 13, 17, 21, and 25 °C. Seedlings were harvested when the second leaf began to emerge. Coleoptiles and first seedling leaves were examined for the presence of lesions caused by S. nodorum. First leaves were incubated on Bannon's medium for 2 weeks, after which they were examined for pycnidia of S. nodorum. Transmission to the coleoptile occurred at all temperatures, but decreased from 100% to 72% as temperature increased from 9 to 25 °C. Transmission to the first leaf was less, dropping from 37% to 2% as temperature increased from 9 to 25 °C. At least 44% of infected first leaves were symptomless at all temperatures, with 96% of infected leaves showing no symptoms at 25 °C. Transmission to seedling leaves occurred over a broad temperature range. Under the high densities at which wheat is sown, a significant number of infected seedlings per unit area may originate from relatively low initial seed infection levels and transmission efficiencies.
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  • 59
    ISSN: 1573-8264
    Keywords: carboxylation efficiecy ; carotenoids ; chlorophyll ; growth analysis ; net photosynthetic rate ; photochemical efficiency ; ribulose 1,5-bisphosphate carboxylase/oxygenase ; stomatal conductance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat plants were grown from sowing to day 18 in 26-dm3 chambers at three different CO2 concentrations: 150 (-CO2), 350 (C, control), 800 (+CO2) μmol mol-1. Afterwards, plants of the three variants were grown at the same natural CO2 concentration. Plant characteristics were measured just before the transfer (0 days after CO2 treatment, DAT), and at 5 – 8 DAT on the 1st leaf, and at 12 – 22 DAT on the 4th leaf. Decreased or increased CO2 concentrations caused acclimations which persisted after transplantation to natural CO2 concentration. At 5 – 8 DAT, stomatal density, stomatal conductance (gs), CO2 saturated net photosynthetic rate (PNsat0), radiation saturated net photosynthetic rate (PNsat1), and carboxylation efficiency (τ) were higher in -CO2 plants and lower in +CO2 plants than in C plants. As compared with C plants, the photochemical efficiency (α) was lower in -CO2 and higher in -CO2 plants, however, chlorophyll (Chl) a, Chl b, Chl a–b and carotenoid contents were lower in both -CO2 and +CO2 plants. On the 4th leaf, which emerged on plant after finishing CO2 treatments, at 12 – 22 DAT, no differences in stomatal density and g, between treatments were observed. In -CO2 plants, pigment content and PNsat0 were higher, α was lower, and PNsat1 and τ were not different from C plants. In contrast, in +CO2 plants, pigment content, PNsat1 and τ were lower, and PNsat0 and α were unchanged. Leaf area, dry mass, and tiller development increased in +CO2 plants and decreased in -CO2 plants. In the interval between 8 and 22 DAT, lower net assimilation rate in +CO2 than in -CO2 plants was observed.
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  • 60
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    Biologia plantarum 43 (2000), S. 523-528 
    ISSN: 1573-8264
    Keywords: accumulation ; grains ; lithium ; mobility ; phloem ; potassium ; rubidium ; sodium ; strontium ; Triticum aestivum ; xylem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The alkali metals cesium, rubidium, lithium and sodium were introduced together with strontium via flaps into leaf laminas or into the stem of maturing, intact winter wheat (Triticum aestivum L. cv. Arina) grown in a field. Long-distance transport of these elements and the influence of the application date and of different application positions were investigated. The phloem-immobile Sr served as a marker for the distribution of the xylem sap in the plants. Dry matter accumulation in the grains and the transpiration per shoot were not markedly affected by the treatments as compared to control plants. The phloem mobility was rather high for Cs and Rb. Li was almost immobile in the phloem (similarly to Sr). An application into the cut stem xylem below the second leaf node contributed more to the contents in the grains than an application into the flag leaf. An earlier feeding date led to a higher accumulation in the grains. The marked losses of the elements applied during maturation (most pronounced for Li) can be explained by leakage in the rain.
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  • 61
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    Biologia plantarum 43 (2000), S. 61-66 
    ISSN: 1573-8264
    Keywords: lipids ; polypeptide pattern ; salt stress ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat seedlings of a salt tolerant cultivar were grown hydroponically in presence and absence of 100 mM NaCl. Roots were harvested, and the plasma membrane (PM) fraction was purified. PM ATPase required a divalent cations for activity (Mg 〉 Mn 〉 Ca 〉 Co 〉 Zn 〉 Ni 〉 Cu), and it was further stimulated by monovalent cations (K 〉 Rb 〉 NH4 〉 Li 〉 choline 〉 Cs). The pH optima were 6.0 and 5.6 in absence and presence of 25 mM KCl, respectively. The enzyme was sensitive to vanadate and DCCD but insensitive to azide, oligomycine and nitrate. The enzyme displayed a high preference for ATP but was also able to hydrolyze other nucleotide tri- and diphosphates. The enzyme activity showed a simple Michaelis-Menten kinetics for the substrate Mg2+-ATP in both control and salt exposed roots. The polypeptide patterns of control and salt stressed PM fractions, detected by SDS-PAGE, were very similar. NaCl substantially reduced the PM ATPase specific activity, whereas it had little effect on the apparent Km for Mg2+-ATP. Since the root PM ATPase of salt sensitive and resistant genotypes responded similarly to salinity stress, it seems unlikely that the mechanism of salt tolerance in wheat is primarily based on differences in PM ATPase characteristics.
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  • 62
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    Biologia plantarum 43 (2000), S. 309-311 
    ISSN: 1573-8264
    Keywords: grains ; maturation ; radioisotopes ; transport ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Steam-girdling experiments with detached wheat shoots showed that cesium was eliminated from the xylem sap and loaded into the phloem during acropetal transport. This transfer is important for the accumulation of cesium (especially also of the radiopollutants 134Cs and 137Cs) in maturing wheat grains.
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  • 63
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    Biologia plantarum 43 (2000), S. 559-562 
    ISSN: 1573-8264
    Keywords: phenolics ; susceptibility ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyphenol oxidase activity was higher in resistant wheat cultivar ACC-8226 than in susceptible cultivar MP-845 in control sets and after inoculation of Alternaria triticina. However, similar polyphenol oxidase isozyme pattern was found in control and inoculated sets of both the cultivars, but the band intensity was higher after inoculation. Three and four peroxidase isozymes were found in ACC-8226 and MP-845, respectively. An extra peroxidase isozyme band was observed in both the cultivars after inoculation. The results suggest an active role of peroxidase and polyphenol oxidase in defence mechanism of wheat plants.
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  • 64
    ISSN: 1573-8264
    Keywords: ascorbic acid ; ascorbate peroxidase ; active oxygen species ; catalase ; hydroger ; peroxide ; oxidative stress ; superoxide dismutase ; temperature stress ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An experiment was conducted with three wheat (Triticum aestivum L.) genotypes C 306, HD 2285 and HD 2329 (differently susceptible to water and temperature stress) to study the extent of oxidative injury and activities of antioxidant enzymes in relation to heat stress induced by manipulating dates of sowing. Increase in temperature by late sowing significantly decreased leaf relative water content (RWC), ascorbic acid content, and increased H2O2 content and lipid peroxidation in all the genotypes at 8 and 23 d after anthesis. Temperature tolerant genotypes C 306, closely followed by HD 2285 were superior to HD 2329 in maintaining high RWC, ascorbic acid content, and lower H2O2 content and lipid peroxidation (malondialdehyde content) under high temperature (late sowing) at the two stages. Activities of superoxide dismutase and catalase were highest in HD 2285 followed by C 306 and minimum in HD 2329 while ascorbate peroxidase activity was highest in C 306.
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  • 65
    ISSN: 1573-8264
    Keywords: active oxygen species ; catalase ; chloroplast ; glutathione reductase ; lipid peroxidation ; nitrate reductase ; proline ; superoxide dismutase ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We induced an oxidative stress by means of exogenous hydrogen peroxide in two wheat genotypes, C 306 (tolerant to water stress) and Hira (susceptible to water stress), and investigated oxidative injury and changes in antioxidant enzymes activity. H2O2 treatment caused chlorophyll degradation, lipid peroxidation, decreased membrane stability and activity of nitrate reductase. Hydrogen peroxide increased the activity of antioxidant enzymes, glutathione reductase and catalase. These effects increased with increasing H2O2 concentrations. However, no change was observed in the activity of superoxide dismutase and proline accumulation.
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  • 66
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    Biologia plantarum 43 (2000), S. 257-262 
    ISSN: 1573-8264
    Keywords: nitrate assimilation ; Triticum aestivum ; Zn toxicity ; Cu toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of Zn2- on nitrate reductase (NR, EC 1.6.6.1) activity was studied in botá wheat (Triticum aestivum cv. Oasis) leaves and in the NR enzyme partially purified from wheat leaves. Leaf segments were floated on 0 to 5 mM ZnSO4 solutions (pH 6.0) for 24 h under continuous light. Zn2- at 250 μM decreased NR activity and increased membrane permeability. However, parameters of cellular oxidative damage were scarcely affected by Zn2- treatments. Accordingly, the decrease of NR activity induced by Zn2- was not prevented by benzoate (a scavenger of oxygen radicals). The effect of Zn2- was dependent on leaf age: it decreased NR activity in mature but not in young leaves. Zn2 inhibited the partially purified NR. This inhibition was not reversed by either co- or post-incubation with cysteine, and the amount of -SH groups of the purified NR was not affected by Zn2+ indicating that Zn2- inhibition does not involve key -SH groups of the enzyme. However, o-phenantroline both prevented and reversed Zn2+-induced NR inhibition. We concluded that the effect of Zn2+ on NR activity in vivo is not associated with an increase in active oxygen generation and involves a direct and reversible inhibition of the enzyme.
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