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  • Ultrastructure  (410)
  • 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring
  • Seismology
  • Springer  (428)
  • INGV  (13)
  • Springer Science+Business Media B.V.  (4)
  • Dover Publ.
  • 2010-2014  (25)
  • 1975-1979  (418)
  • 1950-1954  (4)
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Year
  • 1
    Publication Date: 2020-07-07
    Description: In questo lavoro viene descritta l’installazione di una rete mobile nell’area dei Monti Nebrodi in seguito all’evento del 23-06-2011 di Ml = 4.6 e come tale intervento ha contribuito al miglioramento della localizzazione delle sorgenti sismiche soprattutto nella determinazione della profondità degli eventi. Verranno anche presentati i risultati delle localizzazioni ottenute attraverso l’integrazione dei dati acquisiti durante questa campagna, con quelli della rete sismica permanente dell’INGV-Osservatorio Etneo ( INGV -OE).
    Description: Istituto Nazionale di Geofisica e Vulcanologia
    Description: Published
    Description: 1-24
    Description: 4IT. Banche dati
    Description: N/A or not JCR
    Description: open
    Keywords: Rete Sismica Mobile ; Nebrodi ; Sciame ; Localizzazione ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.10. Instruments and techniques
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 2
    Publication Date: 2017-04-04
    Description: The May 20, 2012, Emilia Ml 5.9 earthquake was followed by some major aftershocks, well recorded by a large number of temporary stations that were installed to monitor the sequence. These additional recordings allowed us a thorough testing of the performance of the ShakeMap—a procedure designed to provide rapid information on the experienced ground motion. We found that the shakemaps for the May 29, 2012, Ml 5.8 earthquake, obtained using the permanent stations only, underestimate significantly the ground motion computed with the highest station density, especially for PSA at long periods (T=3.0 s). This low-frequency motion is controlled primarily by the surface waves recorded in the Po plain: the observed site effects are likely not accounted properly by the site correction coefficient based on Vs30 as implemented in the ShakeMap procedure. The shakemaps determined during the seismic sequence have been included in an Italian national law that was passed after the 2012 earthquake. According to this law, the factories safety verifications were bound to the comparison between the shakemaps and the design acceleration required by the current national seismic code.We then decide to appraise the impact of the shakemap accuracy on the law provisions. Following the law ecommendations, we have estimated the possible errors resulting from the incomplete evaluation of the ground shaking: our results show that, if the complete dataset were available at the time of the law approval, the number of buildings for which the safety check was required would have been significantly smaller.
    Description: Published
    Description: 2147-2164
    Description: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Description: 5T. Sorveglianza sismica e operatività post-terremoto
    Description: JCR Journal
    Description: restricted
    Keywords: Emilia earthquake ; Shakemap ; Strong ground motion ; Seismic hazard ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 3
    Publication Date: 2013-04-18
    Description: Twenty-five lava fountains occurred on Mt. Etna from January 2011 to April 2012. In summer 2012 volcanic activity resumed in a milder form within the Bocca Nuova crater, before it came to an essential halt in August 2012. All these unrests offer rich material for testing automatic procedures of data processing and alert systems, running 24/7, in the context of volcano surveillance. We focus on the seismic background radiation – volcanic tremor – which plays a key role in the monitoring of Mt. Etna. Since 2006 a multistation alert system has been established in the INGV operative centre of Catania exploiting STA/LTA ratios. Besides, also the spectral characteristics of the signal, which change correspondingly to the type of volcanic activity, can be exploited for warning purposes. Here we apply Self Organizing Maps and Fuzzy Clustering which offer an efficient way to visualize signal characteristics and its development with time. All these techniques allow to identify early stages of eruptive events, and automatically flag a critical status before this becomes evident in conventional monitoring techniques. Changes of tremor characteristics are related to the position of the source of the signal. The location of the sources exploits the distribution of the amplitudes across the seismic network. The locations were extremely useful for warning, throughout both the flank eruption in 2008 as well as the 2011 lava fountains, during which a clear migration of tremor sources towards the eruptive centres could be noticed in advance. The location of the sources completes the picture of an imminent volcanic unrest, and corroborates early warnings flagged by the changes of signal characteristics. Real time data processing requires computational efficiency, robustness of the methods and stability of data acquisition. The amplitude based multi-station approach is not sensitive to the failure of single stations and therefore offers a good stability. The single station approach, exploiting unsupervised classification techniques, limits logistic efforts, as only one or few key stations are necessary. Both strategies have proven to be insensitive to disturbances (undesired transients like earthquakes, noise, short gaps in the continuous data flow). False alarms were not encountered so far. Stable data acquisition and processing come with a properly designed data storage solution. The reliability of data storage and its access is a critical issue. A cluster architecture has been realized for failover protection, including a Storage Area Network system, which allow easy data access following predefined user policies. We present concepts of the software architectures deployed at INGV Osservatorio Etneo in order to implement this tremor-based multi approach system. We envisage the integration of seismic data and those originating from other scientific fields (e. g., volcano imagery, geochemistry, deformation, gravity, magneto-telluric). This will facilitate cross-checking of evidences encountered from the single data streams, in particular allow their immediate verification with respect to ground truth.
    Description: Published
    Description: Nicolosi (Catania, Italy)
    Description: 1.4. TTC - Sorveglianza sismologica delle aree vulcaniche attive
    Description: 5.6. TTC - Attività di Sala Operativa
    Description: open
    Keywords: Etna, Volcanic tremor ; Volcano monitoring, Pattern recognition ; Self Organizing Map, Fuzzy clustering ; Data acquisition ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.08. Volcano seismology ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring ; 05. General::05.01. Computational geophysics::05.01.01. Data processing ; 05. General::05.01. Computational geophysics::05.01.04. Statistical analysis
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: Oral presentation
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  • 4
    Publication Date: 2021-05-12
    Description: In questo lavoro si presentano i risultati di uno studio sulle caratteristiche del rumore sismico in prossimità del rilevatore di onde gravitazionali VIRGO (Cascina, Pisa), con particolare riferimento alle vibrazioni associate all’azione di un vicino parco eolico. La valutazione delle componenti spettrali del rumore verosimilmente indotte dagli aerogeneratori è stata effettuata mediante (i) Misure dirette alla base di una turbina, (ii) Correlazione fra le ampiezze spettrali del rumore e la velocità del vento; (iii) Determinazione delle proprietà direzionali da misure multicanale, (iv) Misura dell’attenuazione del segnale con la distanza dal parco eolico. Il disturbo provocato dagli aerogeneratori è particolarmente energetico alla frequenza di 1.7 Hz e, in particolari condizioni, è stato osservato fino a distanze di 11 km dal Parco Eolico. Il decadimento spaziale delle ampiezze ha un andamento complesso, che può essere interpretato in termini di una combinazione fra onde superficiali e onde di volume rifratte ad un’interfaccia profonda (~800 m) fra i sedimenti plio-pleistocenici ed i calcari Miocenici. La risposta locale nei dintorni dell’interferometro è stata investigata utilizzando la tecnica dei rapporti spettrali H/V. Si sono così evidenziate due bande di amplificazione imputabili ad effetti di risonanza legati alla geologia a scala locale: il primo intorno alla frequenza di 0.35 Hz, il secondo fra 0.7 e 2.0 Hz. Entrambi i picchi risultano essere essenzialmente omogenei in tutta l’area di studio, a conferma della sostanziale uniformità della struttura geologica.
    Description: Published
    Description: 1-30
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: N/A or not JCR
    Description: open
    Keywords: Seismic Noise ; Array Seismology ; Gravity Waves ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.09. Waves and wave analysis
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 5
    Publication Date: 2020-10-26
    Description: One of the main objectives of the ITACA (ITalian ACcelerometric Archive) strong motion database, promoted by the Italian Department of Civil Protection, was to improve the characterization of the recording sites from a geological and geophysical point of view and to provide their seismic classification according to the seismic norms pertinent to Italy, namely the Eurocode 8 and the National Technical Norms for Constructions. A standard format to summarize the available information for the recording stations was first produced, in terms of a technical report dynamically linked to the database, i.e., some of the relevant information is automatically updated when the corresponding fields of the database are modified. Then, an important activity of collection, qualification and synthesis of available data was carried out, especially for stations that recorded the strongest earthquakes in Italy in the last 40 years, and for which a relevant number of studies have been published. In spite of this activity, among the more than 700 strong motion stations present in the ITACA database, only a limited number of them could be characterized by quantitative information on subsurface soil properties. For this reason, a dual seismic site classification criterion was implemented, either based on the standard Vs,30 scheme, or, in the absence of such information, based on an expert opinion supported by shallow geology maps, mostly at 1:100,000 scale, and when available on the H/V ratios calculated on recordings. Owing to the relevance in the Italian geographic and morphological context, a special care was also given to the topographic classification of stations, based on suitable criteria developed within a GIS environment.
    Description: Published
    Description: 1779-1796
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: JCR Journal
    Description: open
    Keywords: ITACA database ; Strong motion station ; General characterization ; Site classification ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 05. General::05.02. Data dissemination::05.02.02. Seismological data ; 05. General::05.09. Miscellaneous::05.09.99. General or miscellaneous
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 6
    Publication Date: 2020-12-07
    Description: We present a methodology for determining the elastic properties of the shallow crust from inversion of surface wave dispersion characteristics through a fully nonlinear procedure. Using volcanic tremor data recorded by a small-aperture seismic array on Mount Etna, we measured the surface waves dispersion curves with the multiple signal classification technique. The large number of measurements allows the determination of an a priori probability density function without the need of making any assumption about the uncertainties on the observations. Using this information, we successively conducted the inversion of phase velocities using a probabilistic approach. Using a wave-number integration method, we calculated the predicted dispersion function for thousands of 1-D models through a systematic grid search investigation of shear-wave velocities in individual layers. We joined this set of theoretical dispersion curves to the experimental probability density function (PDF), thus obtaining the desired structural model in terms of an a posteriori PDF of model parameters. This process allowed the representation of the objective function, showing the non-uniqueness of the solutions and providing a quantitative view of the uncertainties associated with the estimation of each parameter. We then compared the solution with the surface wave group velocities derived from diffuse noise Green’s functions calculated at pairs of widely spaced (~5–10 km) stations. In their gross features, results from the two different approaches are comparable, and are in turn consistent with the models presented in several earlier studies.
    Description: Published
    Description: 335-346
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: 1.4. TTC - Sorveglianza sismologica delle aree vulcaniche attive
    Description: JCR Journal
    Description: restricted
    Keywords: Surface waves ; Volcanic tremor ; Dispersion curves ; Nonlinear inversion ; Etna volcano ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.09. Waves and wave analysis
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 7
    Publication Date: 2017-04-04
    Description: In questo lavoro viene descritto il complesso sistema di acquisizione dati della RSM [vedi D’Alema et al., 2011 - in questo volume], costituita da 58 stazioni collegate in tempo reale e 13 stazioni dial-up. I dati delle stazioni in tempo reale sono acquisiti con il programma Seiscomp31; la detezione degli eventi sismici viene eseguita con il programma Earthworm ed infine l’analisi e l’interpretazione degli eventi viene effettuata attraverso il programma SacPicker di Daniele Spallarossa [vedi Spallarossa, 2011 - in questo volume]. La parte di rete dial-up è basata sul sistema Lennartz Mars882 ed è configurata in modo autonomo dalla rete in tempo reale. I dati delle due reti vengono successivamente uniti in un unico dataset ai fini di una interpretazione interattiva congiunta.
    Description: Published
    Description: 124-127
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: N/A or not JCR
    Description: open
    Keywords: rete sismica ; ancona ; acquisizione dati ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.10. Instruments and techniques
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 8
    Publication Date: 2017-04-04
    Description: Da alcuni anni l’INGV e la Regione Marche collaborano nell’azione di monitoraggio sismico del territorio regionale. Nella sede di Ancona del CNT sono acquisiti in tempo reale i segnali sismici di circa 80 stazioni dell’Italia centro-orientale. Si è reso pertanto necessario sviluppare applicativi utili al controllo degli apparati che compongono il sistema di monitoraggio. In particolare, vengono controllati: 1) lo stato di funzionamento delle trasmissioni radio ed ethernet; 2) lo stato dell’alimentazione delle stazioni e il numero di satelliti ricevuti dagli apparati GPS; 3) la quantità di segnale sismico archiviato e i gaps del segnale continuo; 4) i livelli di rumore di fondo e la qualità del segnale sismico.
    Description: Published
    Description: 104-107
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: N/A or not JCR
    Description: open
    Keywords: rete sismica ; ancona ; centro acquisizione ; telecomunicazioni ; qualità segnali ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.10. Instruments and techniques
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 9
    Publication Date: 2017-04-04
    Description: A Pilot Project for CO2 injection and storage was proposed for a gas storage area located at Cortemaggiore (Piacenza), in northern Italy. This project is conducted both to verify the injection techniques and to analyze the potentiality of CO2 as a cushion gas. Starting from 2004, a series of analysis has been conducted to verify suitability and feasibility of this operation. The injection phase will be preceded by a passive seismic monitoring in order to measure the background seismicity of the area. Seismic monitoring will be carried out during the 3 years of the injection phase and will continue also for a control period of 2 years, following the working phase. The Milano - Pavia Department of the Istituto Nazionale di Geofisica e Vulcanologia is in charge of the surface seismic monitoring. To study the background seismicity a microseismic network composed by 7 seismic stations has been realized. On February 2010, a first test phase has been conducted for 3 sites. The network was completed with 4 more stations on May 2010. All stations are composed by a 24-bit digital recorder (Lennartz M24/NET) with GPS time signal. The study area is characterized by a very high anthropic and industrial noise. In order to improve the quality of the seismic signals, 4 stations have been installed in a 100 m deep borehole. The seismic sensors (Lennartz LE-3D/BH for the borehole and LE-3Dlite MKI for the installation at the surface) have similar technical characteristics with 1 Hz free period, cutoff frequency at 80 Hz and dynamic range of 136 dB. In this first stage we analyzed the microseismic noise level and evaluated the detection capability of the network. Using the RMS measurements the borehole stations indicate a reduction on the noise by a factor of 2.5. A more detailed analysis, performed using the density function distribution of the power spectra, evidences a 10 dB gain for the borehole stations in the frequency band 1 - 10 Hz. Noise measurements have been used also to determine the minimum magnitude for the events detection. Using a point source model to simulate seismic events, we verified the expected detection levels by comparing the estimates obtained with the simulation and the local events recorded by the seismic network.
    Description: Published
    Description: 12
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: N/A or not JCR
    Description: open
    Keywords: seismic monitoring ; gas storage ; micro-seismicity ; 04. Solid Earth::04.02. Exploration geophysics::04.02.99. General or miscellaneous ; 04. Solid Earth::04.02. Exploration geophysics::04.02.06. Seismic methods ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.10. Instruments and techniques
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 10
    Publication Date: 2017-04-04
    Description: Nell’ambito del progetto FIRB-Airplane [“Piattaforma di ricerca multidisciplinare su terremoti e vulcani”, fondi MIUR 2007-2011 responsabili: Cocco, Amato e Stucchi1] dalla seconda metà del 2009 è stata installata una rete densa di stazioni sismiche nell’area dell’Alta Val Tiberina (AVT, Figura 1), i cui dati in continuo vengono trasmessi alla sede di Ancona del CNT attraverso una dorsale Wi-Fi HYPERLAN [Monachesi e Cattaneo, 2010]. La rete è stata progettata cercando di rispettare alcuni criteri, tra cui: controllo continuo della funzionalità delle singole stazioni, flessibilità nella scelta dei siti, riduzione del rischio di fulminazioni, possibilità di abbinare stazioni sismiche e stazioni geodetiche. A tal fine sono stati adottati strumenti a basso consumo per quel che riguarda acquisitori e sistemi di trasmissione, accompagnati dall’utilizzo di sistemi di alimentazione autonomi e sistemi di telecontrollo
    Description: Published
    Description: 91-93
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: N/A or not JCR
    Description: open
    Keywords: rete sismica ; alta val tiberina ; sistemi alimentazione ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.10. Instruments and techniques
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 11
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    Springer
    Publication Date: 2017-04-04
    Description: n/a
    Description: Published
    Description: 125-127
    Description: 3.3. Geodinamica e struttura dell'interno della Terra
    Description: restricted
    Keywords: Core-mantle boundary topography ; Seismology ; 04. Solid Earth::04.01. Earth Interior::04.01.03. Mantle and Core dynamics
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: book chapter
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  • 12
    Publication Date: 2017-04-04
    Description: Attualmente presso la sede di Ancona del CNT sono acquisiti i dati relativi a 58 stazioni in realtime ed a 13 stazioni dial-up. In particolare la sede di Ancona ha la funzione di server nei confronti della sala sismica nazionale per 28 stazioni in realtime e client per le rimanenti 30. Le stazioni dial-up hanno una connessione con radio modem (8) o con modem GSM (5). Gli acquisitori gestiti sono: GAIA1 e GAIA2, Trident/Cygnus, HDR24 e Lennartz Mars88/MC/RC. I sensori sono: Trillium 40-120-240s; Lennartz LE3D- 5s/1s/lite/BH, Mark L4-3D, Episensor ES-T. Il sistema di acquisizione dati è basato su Seiscomp31, mentre la detezione degli eventi avviene tramite Earthworm
    Description: Published
    Description: 19-21
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: N/A or not JCR
    Description: open
    Keywords: rete sismica ; monitoraggio ; marche ; alta val tiberina ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.10. Instruments and techniques
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 13
    Publication Date: 2017-04-04
    Description: L’utilizzo di sensori in pozzo ha l’obiettivo di migliorare il rapporto segnale-disturbo per poter registrare segnali di eventi sismici in zone dove le registrazioni in superficie sono corrotte dai disturbi. Infatti, le registrazioni superficiali sono affette dal noise sismico ambientale il quale è particolarmente elevato in zone industrializzate a frequenze generalmente superiori ad 1 Hz [McNamara and Buland, 2004]. La RSN dell’INGV, gestita dal CNT [vedi Delladio, 2011 - in questo volume], è dotata attualmente di 6 sensori in pozzo, ubicati nelle seguenti località: Castelleone (CR), Imola (BO), Bellaria (RN), Senigallia (AN), Sansepolcro (AR), Città di Castello (PG). Le prime 4 località si trovano in pianura padana (Castelleone e Imola) e sulla costa adriatica (Bellaria e Senigallia): in questi siti l’utilizzo di un sensore in pozzo ha lo scopo di ottenere registrazioni in cui i disturbi antropici possano essere significativamente abbattuti, in quanto le aree citate sono tra le più industrializzate e urbanizzate del territorio nazionale [Marzorati and Bindi, 2006]. I pozzi di Sansepolcro e Città di Castello rientrano nell’area di interesse della Faglia Alto Tiberina, in cui terremoti di piccolissima magnitudo vengono registrati solo in siti particolarmente silenziosi dal punto di vista del noise sismico ambientale; perciò i due sensori in pozzo hanno lo scopo di avere un punto di osservazione più vicino alla sorgente dei segnali che si vogliono registrare. Nei prossimi paragrafi sono mostrati alcuni esempi utili per poter valutare l’efficienza dei sensori in pozzo.
    Description: Published
    Description: 72-75
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: N/A or not JCR
    Description: open
    Keywords: sensori ; pozzo ; borehole ; noise sismico ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 14
    Publication Date: 2017-04-04
    Description: In this paper the history of the Mobile Seismic Network of the Osservatorio Vesuviano (at present Department of Napoli of the Istituto Nazionale di Geofisica e Vulcanologia – INGV) is described. The instrumental development and the main research and monitoring activities since the early 1980’ are reported. Starting from the analog stations with magnetic tape recording to the first digital stations with trigger recording, until the modern multichannel 24bit devices, the technological development of the last 30 years has given a strong contribution to increase the knowledge in the seismology and seismotectonics fields. The Mobile Seismic Network has been engaged in active and passive seismic studies both on volcanic and tectonic areas. Particularly, the contribution of the Mobile Seismic Network for the monitoring of Neapolitan active volcanoes has been very important to improve the Permanent Network. During seismic crises high quality data have been gathered allowing detailed analyses of the seismic activity. Moreover, the time synchronization by means of GPS time code allowed the deployment of the Mobile Network abroad and the collaboration with the main international research institutes. At present, the Mobile Seismic Network of the Osservatorio Vesuviano is developing on multichannel acquisition systems, also in array configuration, to gathered simultaneously seismic signals with a large frequency band.
    Description: Published
    Description: 1-51
    Description: 1.4. TTC - Sorveglianza sismologica delle aree vulcaniche attive
    Description: 3.2. Tettonica attiva
    Description: N/A or not JCR
    Description: open
    Keywords: mobile network ; Vesuvius ; Campi Flegrei ; data acquisition ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.08. Volcano seismology ; 04. Solid Earth::04.06. Seismology::04.06.10. Instruments and techniques ; 04. Solid Earth::04.07. Tectonophysics::04.07.02. Geodynamics
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 15
    Publication Date: 2017-04-04
    Description: This paper describes the analyses of the single-station ambient-vibration measurements performed on the Italian accelerometric network to detect site resonance phenomena potentially affecting earthquake recordings. The use of low cost, high quality microtremor measurement can be helpful to discriminate among soil classes, since several classification schemes based on resonance frequencies were proposed in the last decades. Operatively, in the framework of the Italian Strong Motion Database project (DPC-INGV 2007–2009 S4; http://esse4.mi.ingv.it), soil resonance frequencies have been evaluated from more than 200 ambient vibration measurements in correspondence of accelerometric stations included in ITACA (http://itaca.mi.ingv.it/ItacaNet/). The noise recordings have been analyzed using the same numerical protocol in order to standardize the results. Particular attention has been paid to evaluate the quality of measurements and to develop an on-purpose mathematical tool to automatically estimate the peaks in the horizontal-to-vertical spectral ratio (HVSR) curve. The reliability of the resonance frequencies from HVSR has been tested by comparing estimates provided by independent methods (modeling or earthquake recordings). The test confirmed the reliability of the microtremor HVSR for assessing the resonance frequencies of the examined sites.
    Description: Published
    Description: 1821-1838
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: JCR Journal
    Description: open
    Keywords: Ambient vibration measurements ; Strong motion database ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 16
    Publication Date: 2017-04-03
    Description: The idea of this Special Issue comes from a “joint venture” of research groups from several Italian and European Institutions (Istituto Nazionale di Geofisica e Vulcanolo- gia and University of Basilicata, Italy; GeoForschungsZentrum-GFZ, Germany; CETE Méditerranée—LRPC Nice and OCA-UMR Géoazur, France) which carried out a large seismic survey for site effect estimation in the wide area hit by the 2009 L’Aquila, Italy, earthquake (Fig. 1). The Mw 6.3 mainshock of April 6th, 2009, represented the most damaging event in Italy since the 1980 Irpinia earthquake (Mw 6.9). Several large aftershocks (Mw 〉 5) and thousands of smaller events occurred in the following months and the deployment of differ- ent rapid response seismic networks from Italy, France and Germany allowed the scientific community to collect a very large and high quality data set, probably the largest ever acquired during a normal fault seismic sequence (Marzorati et al. 2011).
    Description: Published
    Description: 691-695
    Description: JCR Journal
    Description: restricted
    Keywords: Aquila Earthquakes of April 2009 ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 17
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    Springer Science+Business Media B.V.
    Publication Date: 2017-04-04
    Description: Rapid magnitude estimate procedures represent a crucial part of proposed Earthquake Early Warning Systems. Most of these estimates are fo- cused on the first part of the P-wave train, the earlier and less destructive part of the ground motion that follows an earthquake. Allen and Kanamori [2003] proposed to use the predominant period of the P-wave to determine the magnitude of a large earthquake at local distance and Olivieri et al. [2008] calibrated a specific relation for the Italian region. The Mw 6.3 earthquake that hit Central Italy on April 6, 2009 and the largest aftershocks provide a useful dataset to validate the proposed relation and discuss the risks con- nected to the extrapolation of magnitude relations with a poor dataset of large earthquake waveforms. A large discrepancy between ML estimated by means of τ max evaluation and standard ML (6.8 ± 1.5 vs. 5.9 ± 0.4) suggests using p caution when ML vs. τmax calibrations do not include a relevant dataset of p large earthquakes. Effects from large residuals could be mitigated or removed introducing selection rules on τ function, by regionalizing the ML vs. τmax pp function in the presence of significant tectonic or geological heterogeneity, and using probabilistic and evolutionary methods.
    Description: Published
    Description: 607-614
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: 3.1. Fisica dei terremoti
    Description: JCR Journal
    Description: reserved
    Keywords: Earthquake ; Magnitude ; Earthquake Early Warning Systems ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 18
    Publication Date: 2017-04-04
    Description: During 2007–2008, three CO2 flux surveys were performed on El Chichón volcanic lake, Chiapas, Mexico, with an additional survey in April 2008 covering the entire crater floor (including the lake). The mean CO2 flux calculated by sequential Gaussian simulation from the lake was 1,190 (March 2007), 730 (December 2007) and 1,134 g m−2 day−1 (April 2008) with total emission rates of 164±9.5 (March 2007), 59±2.5 (December 2007) and 109±6.6 t day−1 (April 2008). The mean CO2 flux estimated from the entire crater floor area was 1,102 g m−2 day−1 for April 2008 with a total emission rate of 144±5.9 t day−1. Significant change in CO2 flux was not detected during the period of survey, and the mapping of the CO2 flux highlighted lineaments reflecting the main local and regional tectonic patterns. The 3He/4He ratio (as high as 8.1 RA) for gases in the El Chichón crater is generally higher than those observed at the neighbouring Transmexican Volcanic Belt and the Central American Volcanic Arc. The CO2/3He ratios for the high 3He/4He gases tend to have the MORB-like values (1.41×109), and the CO2/3He ratios for the lower 3He/4He gases fall within the range for the arc-type gases. The high 3He/4He ratios, the MORB-like CO2/3He ratios for the high 3He/4He gases and high proportion of MORB-CO2 (M=25 ±15%) at El Chichón indicate a greater depth for the generation of magma when compared to typical arc volcanoes.
    Description: Published
    Description: 423-441
    Description: 2.4. TTC - Laboratori di geochimica dei fluidi
    Description: JCR Journal
    Description: reserved
    Keywords: El Chichon ; CO2 soil flux ; Crater Lake ; Gas geochemistry ; He-C isotopes ; Fumarolic and bubbling gases ; Tectonics ; 04. Solid Earth::04.04. Geology::04.04.12. Fluid Geochemistry ; 04. Solid Earth::04.05. Geomagnetism::04.05.08. Instruments and techniques ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.08. Volcanology::04.08.01. Gases ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring ; 05. General::05.02. Data dissemination::05.02.01. Geochemical data
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 19
    Publication Date: 2017-04-04
    Description: Il Centro di Ricerche Sismologiche (CRS) dell’Istituto Nazionale di Oceanografia e di Geofisica Sperimentale - OGS è stato istituito con la legge n. 399 del 30 novembre 1989 col fine istituzionale di: “...svolgere, in autonomia scientifica e secondo specifici progetti: ricerche sulla sismicità e sulla sismogenesi dell’Italia nordorientale, gestendo e sviluppando inoltre la connessa rete di rilevamento sismico anche per fini di protezione civile. Nei campi di sua competenza il dipartimento svolge inoltre: attività applicativa; studi e ricerche sulle tecnologie di acquisizione, trasmissione e trattamento del segnale sismico; studi e ricerche per l’affinamento del modello sismotettonico dell’Italia nord-orientale.” Dal 2003, in seguito alla conferenza “Integrating the Seismic Monitoring in Central Europe” tenutasi a Udine e al Workshop “Beyond Frontiers: Seismic Networks in the Southern Alps” organizzato a Trieste, l’OGS ha partecipato al progetto europeo Interreg IIIa Italia/Austria “Reti sismologiche senza frontiere nelle Alpi sudorientali” 1. Il progetto nasce dalla stretta collaborazione esistente tra: • il CRS dell’OGS a Udine; • la Protezione Civile della Regione Friuli-Venezia Giulia (FVG); • l’allora Dipartimento di Scienze della Terra (DST) dell’Università di Trieste (adesso Dipartimento di Geoscienze); • lo Zentralanstalt für Meteorologie und Geodynamik, Hauptabteilung Geophysik di Vienna in Austria (ZAMG); • l’Urad za seizmologijo, geologijo, Agencija Republike Slovenije za okolje, di Lubiana in Slovenia (ARSO). Il terremoto di Bovec, avvenuto in prossimità dei confini tra FVG, Carinzia e Slovenia nel 1998, ha evidenziato l’importanza di un’integrazione e potenziamento delle reti delle diverse nazioni. In particolare, in tale occasione si è evidenziato: l’eterogeneità della strumentazione esistente nelle diverse reti, la mancanza di connessione dedicata tra i centri sismologici anche all’interno della stessa nazione e la mancanza di un sistema di elaborazione ed archiviazione dei dati omogeneo. Lo scopo del progetto è stato quindi l’integrazione transfrontaliera delle reti sismologiche presenti nelle tre nazioni per far fronte alle necessità di protezione civile e per scopi di ricerca scientifica. La disponibilità in tempo reale di forme d’onda dei terremoti permette, infatti, l’analisi immediata dei dati in maniera automatica che fornisce informazioni rapide ed affidabili alle autorità di protezione civile. Gli obiettivi principali raggiunti durante il progetto sono: 1. la creazione di un centro di raccolta dati in tempo reale presso la Sala Operativa Regionale (SOR) di Palmanova della Protezione Civile del Friuli-Venezia Giulia e l’adattamento dei centri raccolta dati del DST, dell’OGS e di Vienna a questi scopi. La duplicazione dei centri di raccolta dati e delle connessioni tra questi garantisce il funzionamento del sistema anche nel caso che uno o più centri siano fuori uso per i danni subiti da un sisma distruttivo; 2. l’individuazione e la realizzazione di uno o più sistemi di connessione dati efficienti e sicuri tra le stazioni ed i centri di raccolta; 3. la ridefinizione della geometria attuale delle reti, in funzione della migliore copertura possibile delle aree potenzialmente pericolose a ridosso dei confini di stato; 4. la programmazione dei protocolli comuni d’intervento in caso di forti terremoti.
    Description: Published
    Description: 38-41
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: N/A or not JCR
    Description: open
    Keywords: seismic monitoring ; OGS ; Northeast Italy ; real time ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.10. Instruments and techniques
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 20
    Publication Date: 2017-04-04
    Description: The aim of this study is the sharing of waveforms recorded by several Istituto Nazionale di Geofisica e Vulcanologia (INGV) temporary stations (managed by the Milan-Pavia section; INGV MI-PV). These stations were installed after the April 6, 2009, Mw 6.3, L'Aquila earthquake (central Italy). The work synthesizes the activities conducted in the field by the INGV MI-PV working group over the three months following the mainshock. The field activities were developed in four different phases that were defined according to their time periods. Starting from April 7, 2009, for the first phase, the temporary stations were installed in correspondence with the more damaged areas. The scope was to record the strongest aftershocks in the days that followed the mainshock. In this phase, the stations were composed of a sixcomponent acquisition system that was coupled with both a weak-motion and a strong-motion sensor. After the first month, the last three phases of installation investigated the seismic responses of sites located in the epicentral area, involving villages within a radius of about 20 km from the epicenter of the April 6 mainshock. In this way, over four specific time-period phases, the stations were installed in sites with different lithological and geomorphological conditions. The instruments worked from April 7 to July 14, 2009; in this period, 9,155 aftershocks (134,262 accelerometric waveforms and 133,242 velocimetric waveforms), with ML ≤5.3 were recorded. This study describes the dataset of these earthquake waveforms recorded with both velocity and acceleration transducers. Selected waveforms are available through ftp://ftp.mi.ingv.it/download/RAIS-TS_rel01/, with their corresponding information concerning instrumental characteristics, installation sites, and earthquakes recorded.
    Description: Published
    Description: 101-113
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: JCR Journal
    Description: open
    Keywords: Seismological data ; L'Aquila earthquake ; Aftershocks ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 21
    Publication Date: 2017-04-04
    Description: In this work we present and discuss the results of ambient seismic noise analyses computed at four sites where seismic stations, managed by the INGV (Italian Institute for Geophysics and Vulcanology) and the DPC (Italian Department of Civil Protection), are installed inside buildings. The experiments were performed considering different types of installation: sensor located at the bottom of a school, directly installed on rock (case 1); sensor located at the bottom of a medieval fortress, built on an isolate hill, directly installed on rock (case 2); sensor installed on the foundations of a medieval fortress, built on an isolate hill (case 3); sensor installed on the foundations of a school, built on alluvial deposits (case 4). Since recent works proposed the use of spectral ratio techniques to study the dynamic characterization of buildings, ambient seismic-noise measurements were performed for each site close to the stations (at the base of the structures), at the top of the structures and outside the buildings. In order to check the source of vibrations both horizontal to vertical spectral ratio (HVNR) and standard spectral ratio (SSR) techniques were applied. For all stations the results from ambient seismic noise were compared to those obtained from earthquakes (HVSR). In order to detect preferential directions of amplification, for each site average HVNRs and HVSRs were computed considering one azimuth for each set of 5°. We obtain different results for different types of installation: in cases 1 and 2, where the sensors are directly installed on rock, the vibrations of the structure do not affect the noise measures performed close to the stations, which show flat HVNR in the whole frequency range: in both cases the eigenfrequency of the building is given by the HVNR calculated from the measures performed at the top of the structure. In cases 3 and 4, where the sensors are installed on the foundations of the considered structures, both the amplification peaks between 5 and 9 Hz (case 3) and between 5.5 and 7 Hz (case 4) include the contribution of the free oscillations of the buildings. In particular, in case 4, HVNRs performed outside building highlight possible soil–structure resonance effects in case of an earthquake.
    Description: Published
    Description: 723-738
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: JCR Journal
    Description: open
    Keywords: site effect ; soil-structure interaction ; spectral ratio techniques ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.10. Instruments and techniques
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 22
    Publication Date: 2017-04-04
    Description: The necessity of a dense network in Northern Italy started from the lack of available data after the occurrence of the 24th November 2004, Ml 5.2, Salò earthquake. Since 2006, many efforts have been made by the INGV (Italian National Institute for Geophysic and Vulcanology), Department of Milano-Pavia (hereinafter INGV MI-PV), to improve the strong-motion monitoring of the Northern Italy regions. This activity led to the installation of a strong-motion network composed by 20 accelerometers, 4 coupled with 20-bits Lennartz Mars88 recorders, 12 coupled with 24-bits Reftek 130 recorders and 4 coupled with 24-bits Gaia2 recorders. The network allow us to reduce, in the area under study, the average inter-distances between strong-motion stations from about 40 km (at November 2004) to 15 km. At present the network includes nine 6-channels stations where velocity sensors work together the strong-motion ones. The data transmission is assured by modem-gsm, with the exception of four stations that send data in real time through a TCP/IP protocol. In order to evaluate different site responses, the stations have been installed both in free field and near (or inside) public buildings, located in the center of small villages. From June 2006 to December 2008 a dataset of 94 events with local magnitude range from 0.7 to 5.1 has been collected. An ad hoc data-processing system have been created in order to provide, after each recorded event, engineering parameters such as peak ground acceleration (PGA) and velocity (PGV), response spectra (SA and PSV), Arias and Housner intensities. Data dissemination is achieved through the web site http://rais.mi.ingv.it, while the waveforms are distributed through the Italian strong motion database (http://itaca.mi.ingv.it).
    Description: Published
    Description: 1091-1104
    Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale
    Description: JCR Journal
    Description: open
    Keywords: North Italy ; Strong motion station ; Data acquisition system ; Seismic networks ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring
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  • 23
    Publication Date: 2017-04-04
    Description: A new method of macroseismic surveys, based on voluntary collaboration through the Internet, has been running at the Istituto Nazionale di Geofisica e Vulcanologia (INGV) since July 2007. The macroseismic questionnaire is addressed to a single non-specialist; reported effects are statistically analysed to extrapolate a probabilistic estimate of Mercalli Cancani Sieberg and European Macroseismic Scale intensities for that observer. Maps of macroseismic intensity are displayed online in almost real time and are continuously updated when new data are made available. For densely inhabited zones, we have received reports of felt effects for even very small events (M=2). Six earthquakes are presented here, showing the ability of the method to give fast and interesting results. The effects reported in questionnaires coming from three towns are carefully analysed and assigned intensities are compared with those derived from traditional macroseismic surveys, showing the reliability of our web-based method.
    Description: Published
    Description: 1.11. TTC - Osservazioni e monitoraggio macrosismico del territorio nazionale
    Description: JCR Journal
    Description: open
    Keywords: Earthquakes ; Macroseismic intensity ; Questionnaire ; Macroseismic effects ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring
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  • 24
    Publication Date: 2017-04-04
    Description: A seismic noise recording experiment has been carried out in the Campi Flegrei volcanic area from March 9 to 26, 2009 in the framework of the INGV-DPC 2007-2009 agreement (Project V1 – UNREST). The project aimed at the realization of an integrated method for the definition of the unrest phases at Campi Flegrei. 21 digital three-component seismic stations equipped with broad band seismometers have been added to the existing 11 digital stations already deployed in the area. The preliminary results show a correlation between the seismic noise level and the anthropic activity, whereas the meteorological conditions seem affecting the low frequency seismic noise. These results are important to define the detection thresholds of the seismic signals generated during a possible renewal phase of the volcanic activity.
    Description: Published
    Description: 1-21
    Description: N/A or not JCR
    Description: open
    Keywords: Campi Flegrei ; seismic noise ; spectral analysis ; wave polarization ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.08. Volcano seismology ; 04. Solid Earth::04.06. Seismology::04.06.09. Waves and wave analysis ; 04. Solid Earth::04.06. Seismology::04.06.10. Instruments and techniques ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 25
    Publication Date: 2017-04-04
    Description: Experimental data and numerical modelling were used to study the effect of local geology on the seismic response of the Catania area. The town extends on a marly clays bedrock and terraced deposits made up by coastal sands and alluvial conglomerates. This sedimentary substratum is deeply entrenched by paleo-valleys filled by lava flows and pyroclastics. Available borehole data and elastic parameters were used to reconstruct a geotechnical model in order to perfome 1D numerical modeling. Seismic urban scenarios were simulated considering destructive (Mw = 7.0), strong (Mw = 6.2) and moderate (Mw = 5.7) earthquakes to assess the shaking level of the different outcropping formations. For each scenario seven real accelerograms were selected from the European Strong Motion Database to assess the expected seismic input at the bedrock. PGA and spectral acceleration at different periods were obtained in the urban area through the equivalent linear numerical code EERA, and contour maps of different levels of shaking were drawn. Standard and horizontal-to-vertical spectral ratios were achieved making use of a dataset of 172 seismic events recorded at ten sites located on the main outcropping lithotypes. Spectral ratios inferred from earthquake data were compared with theoretical transfer functions. Both experimental and numerical results confirm the role of the geological and morphologic setting of Catania. Amplification of seismic motion mainly occurs in three different stratigraphic conditions: (a) sedimentary deposits mainly diffused in the south of the study area; (b) spots of soft sediments surrounded by lava flows; (c) intensely fractured and scoriaceous basaltic lavas.
    Description: In press
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: JCR Journal
    Description: restricted
    Keywords: Scenario earthquake ; 1D modelling ; PGA values ; Earthquake records ; spectral ratios ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion ; 04. Solid Earth::04.06. Seismology::04.06.06. Surveys, measurements, and monitoring ; 04. Solid Earth::04.06. Seismology::04.06.09. Waves and wave analysis
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  • 26
    ISSN: 1432-0827
    Keywords: Bone resorption ; Osteogenesis ; Fish bone ; Osteocytes ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The comparative ultrastructure of fish bone osteogenesis and resorption induced by scale removal was described in the osteocytic (cellular-boned)Carassius auratus and the anosteocytic (acellular-boned)Tilapia macrocephala. Osteocytes, present in osteocytic bone, were lacking in anosteocytic bone. In osteocytic bone the osteoblast secreted a collagenous preosseous matrix in which it became enmeshed and then was termed a preosteocyte. When the preosseous matrix mineralized, the preosteocyte was termed an osteocyte and was completely surrounded by bone. In anosteocytic bone the osteoblasts receded from the mineralizing front and never became trapped as osteocytes. During resorption, types A and B resorptive cells, present in both bone types, invaded the matrix and demineralized the osseous zone. These cells were characterized by large amounts of granular endoplasmic reticulum and intracellular inclusions containing crystal-like material. Although functionally similar to mammalian osteoclasts, these cells lacked a characteristic ruffled border and were not multinucleated. The osteocytes of cellular bone did not appear to be involved during demineralization.
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  • 27
    Electronic Resource
    Electronic Resource
    Springer
    Pure and applied geophysics 117 (1979), S. 1045-1049 
    ISSN: 1420-9136
    Keywords: Seismology ; Surface waves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Description / Table of Contents: Summary Alsop's method (1966) for the non-normal passage of surface waves through a vertical discontinuity is improved by using a new functional to be made stationary on the plane of discontinuity. The modified functional has the dimension of an energy flux and is characterized by the discontinuous surface wave displacements and stresses on the vertical discontinuity. Model calculations which were carried out for SV-waves and Love waves show that the new version of Alsop's method yields in all respects more consistent results than the old one.
    Notes: Zusammenfassung Die Methode von Alsop (1966) für den Durchgang von schräg einfallenden Oberflächenwellen durch eine vertikale Diskontinuität wird durch Verwendung eines neuen Funktionals, das auf der Diskontinuitätsfläche stationär sein soll, verbessert Das modifizierte Funktional hat die Dimension eines Energieflusses und wird durch diejenige Spannungs- und Verschiebungsdiskontinuität charakterisiert, die durch das Oberflächenwellenfeld auf der Diskontinuitätsfläche erzeugt wird. Modellrechnungen für SV-Wellen und Lovewellen zeigen, daß die neue Version von Alsoos Verfahren in jeder Hinsicht konsistentere Ergebnisse als die bisherige liefert.
    Type of Medium: Electronic Resource
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  • 28
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 186 (1979), S. 65-70 
    ISSN: 1432-041X
    Keywords: Sea urchin ; Embryo ; Collagen ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Collagen fibrils with a main period banding of 610 Å and 220 Å in width were observed in the blastocoel of 72-h embryos of the sea urchin,Strongylocentrotus purpuratus. Non-striated fibrils of 50 Å diameter were also observed. The collagen is seen in highest concentration in the vicinity of mesenchyme cells which are richly endowed with endoplasmic reticulum and secretory vesicles. A role for collagen in cell attachment, orientation and spicule formation is discussed.
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  • 29
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 185 (1979), S. 333-346 
    ISSN: 1432-041X
    Keywords: Chick embryo ; Limb bud ; Ultrastructure ; Cell death
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ultrastructural changes in the wing bud afterapical ectodermal ridge (A.E.R.) removal was studied to re-examine the issue of distal mesenchymal cell death. The A.E.R. of the right wing bud was removed microsurgically from chick embryos of stages 18 to 22 (HH 1951). The wing buds were examined at three hour intervals up to twelve hours after the operation with light, transmission and scanning electron microscopy. The main findings were: (1) Immediate and temporary shrinkage of the mesenchymal extracellular space 100 to 150 μm and chromatin condensation in the cells 50 to 75 μm from the wound. (2) Death of ectodermal and mesenchymal cells in the immediate vicinity of the wound. (3) Formation of a single squamous-like layer of mesenchymal cells to cover the wound. (4) Occasional evidence of cell death in the distal mesenchyme at later times after the operation. The pattern of cell death observed suggests only a traumatic etiology, and gives little evidence for the postulated developmental significance of cell death following A.E.R. removal.
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  • 30
    ISSN: 1432-072X
    Keywords: Methanogenium cariaci ; Methanogenium marisnigri ; Marine methanogenic bacteria ; Ultrastructure ; TaxonomyMethanogenium gen. nov.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new genus of marine methanogenic bacteria and two species within this genus are described.Methanogenium is the proposed genus andMethanogenium cariaci the type species. Cells of the type species are Gram-negative, peritrichously flagellated, irregular cocci with a periodic wall surface pattern. Colonies formed by these bacteria are yellow, circular and umbonate with entire edges. The DNA base composition is 52 mol% guanine plus cytosine. Formate or hydrogen and carbon dioxide serve as substrates for growth. Cells ofMethanogenium marisnigri are of similar shape but smaller diameter thanM. cariaci. The colonies ofM. marisnigri are convex, and the DNA base composition is 61 mol % G+C. Formate or hydrogen and carbon dioxide are growth substrates. Sodium chloride is required for growth of both methanogens.
    Type of Medium: Electronic Resource
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  • 31
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 121 (1979), S. 53-59 
    ISSN: 1432-072X
    Keywords: Saccharomyces ; Kluyveromyces ; Ultrastructure ; Ascospore wall ; Taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three types of structure of the ascospore wall were found among the haploid Saccharomyces species examined: a warty wall (S. rouxii), a smooth wall with a single electron-light inner layer (S. bailii) and a smooth wall with a double light inner layer (S. montanus, S. florentinus). The latter type also occurred in Kluyveromyces thermotolerans and K. waltii. In K. fragilis spores the wall had a single light inner layer. The taxonomic implications of these findings were discussed.
    Type of Medium: Electronic Resource
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  • 32
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 122 (1979), S. 17-27 
    ISSN: 1432-072X
    Keywords: Gliding bacteria ; CO2-requiring ; Periodontal disease ; Gram-negative ; Ultrastructure ; Capnocytophaga
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gram-negative, anaerobic gliding bacteria were isolated from normal supragingival plaque and from periodontal lesions. Isolates could be divided into two size classes: small 2.4–4.2 μm×0.38–0.5 μm and large 4.8–5.8 μm×0.42–0.6 μm cells. The outer membrane was either loose-fitting and wavy, or taut, and of variable thickness. An electron-dense fuzz was discernible on several of the isolates. The periplasmic region was of variable electron-density. The genus Capnocytophaga has been proposed for these organisms based on morphological and cultural characteristics.
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  • 33
    ISSN: 1432-0878
    Keywords: Thyroid follicular cells ; Basal lamina, biosynthesis ; Cellular interaction ; Cell culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Porcine thyroid cells were cultured alone or in mixed cultures with mesenchymal cells. The formation of a basal lamina in vitro was investigated ultrastructurally. Follicular reassociation of thyroid cells occurred in both types of culture; however, it was followed by formation of the basal lamina only when mesenchymal cells were present. The present findings suggest an epithelial origin of the basal lamina resulting from an interaction with mesenchymal cells.
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    Cell & tissue research 200 (1979), S. 257-271 
    ISSN: 1432-0878
    Keywords: Anococcygeus muscle ; Innervation ; P-type nerves ; Purinergic nerves ; Ultrastructure
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    Notes: Summary The innervation of the rat anococcygeus muscle has been investigated ultrastructurally following fixation with a modified chromaffin reaction for the demonstration of biogenic amines (Tranzer and Richards, 1976). Three types of nerve profiles were revealed: (1) 60–70 % of the profiles are adrenergic; (2) less than 5% of the profiles appear to be cholinergic; (3) up to 40% of the profiles are distinguished by the presence of a characteristically high proportion of electron-opaque, chromaffin-negative vesicles, 85–110nm in diameter. This third type of profile was not affected by 6-OHDA, and is considered to represent the non-adrenergic, non-cholinergic inhibitory innervation of this tissue. Because of the morphological similarity of this nerve type, apart from the smaller vesicle size, to classical peptidergic nerve endings, they have been termed “small p-type” (sp-type). These results are discussed in relation to a previous report describing only two types of nerve profiles in this tissue (Gillespie and Lüllmann-Rauch, 1974).
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    Cell & tissue research 200 (1979), S. 299-309 
    ISSN: 1432-0878
    Keywords: Germ-free mice ; Inflammation ; Macrophages ; Peritoneum ; Ultrastructure
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    Notes: Summary Peritoneal macrophage ultrastructure was analysed stereologically in germ-free mice given a single intraperitoneal injection of sterile, pyrogen-free saline. Thus the stimulant was non-particulate, non-antigenic and inorganic, and effects of immune reactions were minimal. Macrophages were recovered 1, 6, 24 and 72 h after stimulation. A sequence of structural alterations is reported which may be fundamental to macrophage activation. The plasma membrane and nuclear envelope increased in area within only 1 h of saline injection. During the next 5 h loss of plasma membrane, probably by pinocytosis, caused cellular “rounding” and clear-cut alteration in surface configuration. At the same time lysosome-like granules enlarged but decreased in number. By 24 h most cellular structures and compartments (including the plasma membrane) were enlarged. Morphological evidence of nuclear activation accompanied a rather modest enlargement of the nucleus at this stage. The RER hypertrophied last and must, therefore, be judged sufficient in resident macrophages to support the initial growth response which results after stimulation. Thus hypertrophy was observed eventually in every structure examined. Even the minimally activated macrophages resident in the peritoneum of germ-free mice respond readily to stimulation.
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    Cell & tissue research 200 (1979), S. 291-298 
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    Keywords: Excitatory synapse ; Lateral line-canal organ ; Synaptic body ; Ultrastructure ; Lota lota (Teleostei)
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    Notes: Summary The ultrastructure of the afferent synapse in hair cells of the lateral line-canal organ was studied using different fixation and staining techniques. Glutaraldehyde-fixed tissue without post-osmication, contrasted by section staining with uranyl acetate and lead citrate, was compared with (a) osmium tetroxide-fixed tissue followed by the same staining procedure, and with (b) glutaraldehyde-fixed tissue, block-impregnated with phosphotungstic acid (PTA). The results reveal a pronounced heterogeneity in the composition of the synaptic body, reflecting regional differences in chemical affinity to the fixatives and staining agents. It is proposed that the “intracleft substance”, the synaptic structure defined by the PTA staining technique, is actually due to the glutaraldehyde fixation procedure and is apparently the outer leaflet of the postsynaptic membrane. A special technique that allows alternate sections of a series to be differentially stained for electron microscopy is proposed.
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    Cell & tissue research 200 (1979), S. 193-203 
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    Keywords: Mosquitoes ; Midgut ; Ultrastructure ; Stereology ; Function
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    Description / Table of Contents: Zusammenfassung Morphometrische Untersuchungen des Magenepithels von A. aegypti weisen darauf hin, daß die Verdauung des ersten Blutmahls in eine Reihe von Phasen gegliedert werden kann, die sich mit physiologischen Daten aus der Literatur korrelieren lassen. In einer Phase Ia (0–10 h nach Blutmahl [BM]) entfalten sich die “whorls” des rauhen endoplasmatischen Retikulums, die Golgi-Zonen werden größer, und das basale Labyrinth wird erweitert. Dies stimmt mit Synthese- und Sekretionsprozessen (z.B. peritrophische Membran, Esterasen, Lipasen) und mit Transportvorgängen durch das Magenepithel überein. In Phase Ib (10–20 h nach BM) nehmen die gemessenen zellulären Parameter weiter zu und weisen damit auf hohe Synthese- und Sekretionsaktivitäten (z.B. Verdauungsenzyme) hin. In Phase Ic (20–30 h nach BM) zeigen die an Synthese und Sekretion beteiligten Zellstrukturen, in Übereinstimmung mit der maximalen Proteasenaktivität im Darm, immer noch hohe Werte. Vergrößerte Mikrovillioberfläche, auffallende Lipideinschlüsse und Auftreten von Glykogendepots im Magenepithel deuten auf erhöhte Resorptions-, Speicher- und Transportfunktionen der Zellen hin. In Phase II (30–36 h nach BM) läßt sich anhand der strukturellen Veränderungen der Wechsel von Synthese- und Sekretionvorgängen zu Resorption, teilweiser Speicherung und Transport von Verdauungsprodukten erkennen. In Phase III (36–72 h nach BM) wird der Zellapparat in Übereinstimmung mit dem Ende der Verdauung reduziert. Lipid- und Glykogendepots werden mobilisiert und verschwinden fast vollständig aus den Magenepithelzellen.
    Notes: Summary Morphometric analysis of the epithelial lining of the stomach of A. aegypti suggests that digestion of the first blood meal in the stomach of this species can be viewed as a series of phases that can be correlated with physiological data from the literature. In phase Ia (0–10 h after blood meal [abm]) the whorls of the rough endoplasmic reticulum unfold, the Golgi zones increase, and the basal labyrinth is enlarged. This coincides with processes of synthesis and secretion (e.g., peritrophic membrane, esterases and lipases) and transport by the stomach epithelium. In phase Ib (10–20 habm) the cellular parameters measured further increase, indicating high synthetic and secretory activities (e.g., digestive enzymes). In phase Ic (20–30 habm) cell structures involved in synthesis and secretion still exhibit high values coinciding with maximal activity of proteases in the gut. Enhanced surface area of microvilli, prominent lipid inclusions, and appearance of glycogen deposits in the gut epithelium suggest increased absorption, storage, and transport functions of the stomach cells. In phase II (30–36 habm) structural alteration points to a gradual shift from synthesis and secretion to absorption, partial storage, and transport of nutrients. In phase III (36–72 habm) the cellular apparatus is reduced concomitant with the ending of the digestive cycle. Lipid inclusions and glycogen deposits disappear from the stomach epithelum.
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    Cell & tissue research 198 (1979), S. 373-380 
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    Keywords: Ventral cochlear nucleus ; Calyceal processes ; Flattened synaptic vesicles ; Ultrastructure ; Morphometric study
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    Notes: Summary In this paper we report the appearance of flat vesicle-containing endings in aldehyde-fixed ventral cochlear nucleus of rats with qualitative and quantitative properties suggesting they should be identified as calyceal processes. Their synaptic vesicles are elongate and significantly smaller than the vesicles in the calyces of Lenn and Reese (1966). Therefore these endings are flat vesicular calyceal processes, possibly of inhibitory function.
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    Cell & tissue research 198 (1979), S. 411-426 
    ISSN: 1432-0878
    Keywords: Intermediate lobe ; Hypophysis ; Ultrastructure ; Colour change ; Control mechanisms ; Anolis carolinensis
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    Notes: Summary The ultrastructure of the intermediate lobe of the hypophysis was studied in Anolis carolinensis with the use of a threefold aldehyde fixative. Lizards with a brown skin were selected. The possibility of two types of secretory cells is discussed; neither cell type is innervated. Type I cells are rarely found and contain dense granules approximately 0.3 μm in diameter; Type II cells vary widely in secretory activity. Most of the Type II cells contain a large number of dense secretory granules (up to about 1.3 μm in diameter) almost filling the cytoplasm. Rough endoplasmic reticulum (RER), Golgi apparatus and mitochondria are poorly developed. Only some of these cells show signs suggesting a high secretory activity, namely a well developed RER, Golgi apparatus and numerous mitochondria. In these cells the RER sometimes forms large intracisternal droplets (up to 7 μm in diameter). Two of the animals exhibited a more uniform, high secretory activity. Large (about 2 μm in diameter), pale vacuoles, probably of extracellular character, were found mostly in the vicinity of the perivascular septum. Their role in the release of MSH is discussed. The present data, which are discussed with reference to earlier findings (Forbes, 1972), form the morphological basis for an experimental study on regulation of MSH release (Larsson et al., 1979).
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    Cell & tissue research 198 (1979), S. 455-463 
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    Keywords: Neuromuscular synapses ; Presynaptic density ; Ultrastructure ; Serial sections ; Crustaceans
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    Notes: Summary Representative examples of lowand high-output neuromuscular synapses between motoneuron and distal accessory flexor muscle of the lobster were selected on the basis of their mean quantal content, and subsequently analysed by serial section electron microscopy. The high-output terminal has twice as many synapses as the low-output terminal. However, since the mean surface area of synapses is significantly smaller in the high-output terminal than in the low-output one, the total synaptic surface area between the two types of terminals is similar. Also, though the high-output terminal possesses a greater number of presynaptic dense bodies than its low-output counterpart, the mean number per synapse is similar for the two terminals. The terminals, however, differ significantly in the size of their dense bodies. Thus both the mean and total surface area of these bodies is greater in the high-output terminal than in the low-output one. Moreover, the mean ratio of dense body area to synaptic area is significantly greater for the high-output terminal than for its low-output counterpart. This difference in dense body area parallels the difference in quantal content of synaptic transmission between the lowand high-output terminals and supports the hypothesis that presynaptic densities represent the ultrastructural correlates of transmitter mobilization and/or release.
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    Cell & tissue research 199 (1979), S. 483-492 
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    Keywords: Pituitary gland ; Rat ; Luteotroph cells ; Pimozide ; Ultrastructure
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    Notes: Summary The effects of pimozide, a dopamine receptor-blocking agent, were studied in the pars distalis of the rat. The animals received 100μg/100 g pimozide daily for 5, 10, 15, and 20 days. Pimozide induces striking ultrastructural changes after 5 days of treatment. The number of luteotroph (LTH) cells is significantly increased; they display characteristics of stimulation. The extrusion of granules into the intercellular space via exocytosis is frequently observed. The intercellular spaces are highly dilated, forming a lacunar system filled with an amorphous material, erythrocytes and involuted LTH cells. Transitional stages in the process of involution are observed in LTH cells. Luteotroph cells also form a syncytium. Twenty days after treatment the abovedescribed changes decrease in magnitude. The present findings suggest that pimozide stimulates the mechanism of synthesis and release in the luteotroph cells, an effect that is less evident with longer treatment.
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    Cell & tissue research 199 (1979), S. 519-528 
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    Keywords: Filum terminale ; Spinal cord ; Glial cells ; Ultrastructure ; Frog (Rana pipiens)
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    Notes: Summary The filum terminale, or terminal portion of the spinal cord, was studied in normal adult frogs (Rana pipiens) by means of light and electron microscopy. Astroglial cells are the predominant elements in this region. The rostral portion of the filum terminale consists mainly of (1) a peripheral dense ring of myelinated and some unmyelinated nerve fibers, and processes of astrocytes terminating at the subpial space; (2) an intermediate zone, in which astrocytes are the main cellular elements in addition to a few degenerated neurons; and (3) a central region where the central canal is lined by dark and light ependymal cells. In the caudal portion of the filum terminale, the amount of neuropil is greatly reduced. This region is formed mainly by astrocytic glial cells and very few neuronal elements. The central canal in the caudal portion is located ventrally and contains a lining consisting almost exclusively of dark ependymal cells.
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    Keywords: Pars distalis ; Graft ; Ultrastructure ; Prolactin ; Rat
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    Notes: Summary Intact female rats received transplants of two hypophysial partes distales under the kidney capsule. The plasma levels of prolactin were determined, and the ultrastructure of the grafted gland was studied 15, 45 and 90 days after the operation. Although prolactin levels in the three experimental groups were significantly higher than those in control rats, a decrease in prolactin level was detected in the 45-day samples. Parallel ultrastructural changes suggest that between the 45th and 90th postoperative day a process is initiated leading to hyperplasia and hypertrophy of prolactotrophs.
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    Cell & tissue research 201 (1979), S. 145-158 
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    Keywords: Ultrastructure ; Secretory granules ; Endosalpinx ; Sheep
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    Notes: Summary The ultrastructure of granules in the secretory cells of the endosalpinx of 20 Merino ewes was examined on days 1, 2, 3, and 4 post coitum. Based on the different frequency of granules of different size and structure on days one to four post coitum, one can assume that the ovoid, membrane bounded secretory granules mature in five successive stages. In stage I small, electron-lucent vesicles with a finely granulated and filamentous content become apparent, initially in the neighbourhood of the Golgi complex. In stage II the granules become larger and progressively more eletron-dense by an increase of the granulated material. In stage III, the primarily granulated content forms membranes, that lie in characteristic stacks at different angles to one another, separated by electron-dense areas. This structure fragments when the granule comes to lie beneath the surface of the cell (stage IV) and opens into the lumen of the oviduct, where its content is discharged in membrane fragments or vesicles (stage V). This discharge is mainly observed shortly before the egg is transported into the uterus.
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    Cell & tissue research 202 (1979), S. 479-491 
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    Keywords: Ependyma ; Ultrastructure ; Posterior tela choroidea ; Rhombencephalon ; Rana catesbeiana
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    Notes: Summary The posterior rhombencephalic tela choroidea of the bullfrog was examined by electron microscopy. This membrane, the pia-ependymal roof of the caudal hindbrain, contains a large central region characterized by cuboidal ependymal cells which surround sizable microscopic apertures — the interependymal pores. Ultrastructurally ependymal cells of this area are characterized by infrequent apical microvilli and cilia. They contain irregularly shaped nuclei and few cytoplasmic organelles that are largely apical in position. The most striking feature is an abundance of cytoplasmic filaments forming an extensive cytoskeleton. Laterally these cells are joined by numerous elaborate desmosomes. The majority of the ependymal cells have a basal lamina consisting of single, double, or triple laminae lying parallel to the basal plasma membrane. Several unusual specializations are seen at the margins of the interependymal pores. The ependymal cells have lateral cytoplasmic processes that form the actual border of each pore. These processes originate from the apical surface of the cell and partially enclose an elaborate network of basal lamina associated with the interependymal pores. These findings demonstrate microscopic apertures in the roof of the fourth ventricle in the bullfrog that are associated with an unusual form of supportive ependyma.
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    Cell & tissue research 199 (1979), S. 1-23 
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    Keywords: Neuro-intermediate lobe ; Hypophysis ; Disconnection ; Ultrastructure ; Colour change ; Control mechanisms ; Anolis carolinensis
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    Notes: Summary Morphological changes in the disconnected neuro-intermediate lobe were studied in the lizard, Anolis carolinensis from the 2nd to the 14th post-operative day using a threefold aldehyde fixative (Rodríguez, 1969). Two phases of colour change capacity were exhibited: Phase I started immediately after the transection, lasted for 6 days (mean) and was characterised by an excessive MSH release (brown skin). This phase proceeded gradually into Phase II, designated by an interruption of the MSH release (green skin). The degenerative processes and final elimination of neurons in the disconnected neural lobe propagate in a rostro-caudal direction from the transected area. The aminergic fibres (Type II) disappear within 2 days postoperatively, whereas the degeneration continues for more than 10 days in the peptidergic fibres (Type III, IV and V). The glia cells (ependyma and pituicytes) serve as very active macrophages, engulfing fragments of axons already affected by autolysis and transferring them into glial lysosomes. No apparent morphological changes occur in the shift from Phase I to II. The great majority of the secretory cells of the intermediate lobe are not affected by degenerative processes and appear to be markedly activated by the stalk transection. They exhibit numerous mitochondria, well-developed Golgi complexes forming numerous Golgi vesicles and extensive parallel cisternae of the rough endoplasmic reticulum, sometimes forming large intracisternal droplets (7 μm in diameter). Numerous pale vacuoles are seen, especially toward the intact capillaries, suggesting their coupling to the MSH release by extension of the active membrane area toward the perivascular septum. The number of these vacuoles is very markedly reduced in Phase II (no release), whereas the formation of new granules seems to proceed in early stages. The interruption of the MSH release implies a successive refilling of gradually growing secretory granules and a concomitant reduction in the development of the synthetic apparatus. Mechanisms probably involved in the control of the synthesis and release of MSH are discussed.
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    Cell & tissue research 199 (1979), S. 37-61 
    ISSN: 1432-0878
    Keywords: Avian thymus ; Ultrastructure ; Erythropoiesis
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    Topics: Biology , Medicine
    Notes: Summary Thymus lobes from three species of birds, Quelea quelea, Passer domesticus and Sturnus vulgaris, have been examined ultrastructurally. The component cell types are compared with their counterparts in mammalian thymus glands, and found to be similar. Greater differences exist between small, intermediate and enlarged lobes of one species than exist between species. Developing erythroid cells are present in most enlarging and some enlarged glands. They appear to be developing at the expense of lymphoid cells in some birds. The origin of these cells is discussed. Cells that are possible candidates for the production of some thymic hormones are also described.
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    Keywords: Haemocytes ; Phagocytosis ; Lysosomes ; Acid phosphatase ; Ultrastructure
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    Notes: Summary The plasmatocytes are the major phagocytic blood-cell type in the haemolymph of the wax-moth, Galleria mellonella. In the present study, these cells were allowed to attach to tissue culture dishes for 1 h, rinsed and then incubated with latex beads for up to 72 h. These cells were then fixed for routine transmission electron microscopy and acid phosphatase cytochemistry. Intracellular latex particles were found in tight, ill-defined phagosomes, which were often clearly associated with the Golgi complexes of the plasmatocytes. Fusion of both primary lysosomes and multivesicular bodies with the phagosomes occasionally occurred and this resulted in the accumulation of an acid phosphatase positive reaction product around the test particles. Subsequent experiments showed that this acid phosphatase activity was mainly associated with the primary lysosomes. The results of the lysosome/latex interactions are compared with those obtained from similar studies on the digestive mechanisms in other phagocytes.
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    Cell & tissue research 199 (1979), S. 257-270 
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    Keywords: Liver ; Bile duct ligation ; Tight junctions ; Gap junctions ; Ultrastructure ; Freeze-fracturing ; Morphometry
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    Topics: Biology , Medicine
    Notes: Summary Morphometric analysis of the alterations in interhepatocyte junctions induced by bile duct ligation revealed that after 48 h, during which time the serum bilirubin increased 6 to 8 fold, the membrane area occupied by gap junctions on the apico-lateral and medio-lateral sides decreased from 3.6% in controls to 0.02% in the ligated group. The strands of the zonulae occludentes were reduced in number and showed increased discontinuities. Within 45 min of recanalization of the common bile duct, clusters of particles appeared within and adjacent to the tight junctional areas or in the lateral hepatocyte membrane. Subsequently, the particle aggregations localized in the apico-lateral membrane areas increased in number and size becoming finally indistinguishable from those of controls within 96 h after the onset of recanalization. The zonulae occludentes also rearranged and reestablished their original structure during this period. The serum bilirubin fell to normal within 24 h of recanalization. It is concluded that metabolic and ultrastructural restitution associated with the recanalization of the ligated bile duct have no strict temporal correlation to one another. These studies provide further evidence that alterations in gap and tight junctions induced by pathological processes, e.g. during bile duct ligation, are completely reversible when regeneration occurs.
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    Cell & tissue research 199 (1979), S. 373-386 
    ISSN: 1432-0878
    Keywords: Hypogastric ganglion (rat) ; Vacuolated neurons ; Catecholamine fluorophore ; Ultrastructure ; Testosterone ; Castration
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    Notes: Summary The vacuolated neurons (VN) of the main hypogastric ganglion of the male rat were studied using the formaldehyde-induced fluorescence (FIF) method for the histochemical demonstration of catecholamines. Microspectrofluorimetry was performed to identify the fluorophores and to quantify the FIF. The thiocholine method (Koelle-Gomori) was used to demonstrate acetylcholinesterase activity. The fine structure of the VN was studied using glutaraldehyde/OsO4 fixation. (1) In the untreated adult male rat VN represent only a small population of the total number of hypogastric neurons (0.8–1.2%). The vacuoles are similar to those of the VN from the corresponding female ganglion. (2) The VN are considered to be adrenergic due to the nature of their fluorophore, indicating a primary catecholamine. (3) The first VN appear in the hypogastric ganglia at the age of 7 weeks. After testosterone administration to young rats, VN are found at the age of 4 weeks. (4) The basic fine structure of the VN is similar to that of other ordinary neurons of the hypogastric ganglia. (5) The content of the vacuoles could not be identified. (6) Indications of degeneration were not observed in the VN. (7) The VN are interpreted as being a functional stage of the “short” adrenergic neurons, which are under the control of steroid hormones. (8) Fifteen months after castration, no VN could be found in the hypogastric ganglia, while their number was normal in the corresponding control animals.
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    Cell & tissue research 201 (1979), S. 305-314 
    ISSN: 1432-0878
    Keywords: Cockroach ; Muscle heterogeneity ; Ultrastructure
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    Notes: Summary Ultrastructural studies have been performed upon the posterior coxal depressor muscle (136) and a coxal branch of the main depressor group (135d′) from the mesocoxa of the cockroach, Periplaneta americana. The quantitative stereometric analyses performed have shown the latter muscle to consist of a dorsal band of fibers having 25.5% mitochondria and 13.6% sarcoplasmic reticulum (SR) and T-tubules (TTS), and a ventral group of fibers with only 4.4% mitochondria and 26.6% SR/TTS. The volume fractions characteristic of the ventral fibers of muscle 135 d′ are also typical of muscle 136.
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    Cell & tissue research 201 (1979), S. 369-376 
    ISSN: 1432-0878
    Keywords: Leydig cells ; Filaments ; Golden hamster ; Ultrastructure
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    Notes: Summary A unique cytoplasmic structure has been observed in Leydig cells of the golden hamster. It consists of a laminar core made up of electron dense material surrounded by a filamentous matrix of lower density, and is tentatively called a dense-cored filamentous body (DCFB). DCFBs vary in overall size and in configuration of the centrally disposed dense lamina. They are typically located in the vicinity of the centrosome and the Golgi complex. The body has no limiting membrane, and may be in contact with virtually every type of organelle. The DCFB is well developed in active Leydig cells, whereas it is small in the quiescent stage of the secretory cell. It is likely that the DCFB is a constant organelle in the hamster Leydig cell and may be involved in the physiological function of the Leydig cell, which remains to be specified.
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    Cell & tissue research 202 (1979), S. 203-212 
    ISSN: 1432-0878
    Keywords: Mineralization processes ; Scales ; Teleost fish ; Matrix vesicles ; Ultrastructure
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    Topics: Biology , Medicine
    Notes: Summary In Teleost fish scales, growth and mineralization are continuous. Different mineralization processes can be distinguished. The external layer of the scale is the first to be mineralized and may be classified as the initial calcifying structure of the scale. The initial calcification loci are matrix vesicles of cellular origin always observed during the formation of this layer. This mineralization process takes place progressively, closely following the elaboration of the organic matrix in the scale periphery. The outer limiting and internal layers of the scale are developed after the external layer has been formed. A mineral substance is deposited without the mediation of matrix vesicles, but in contact with the previously mineralized external layer. This type of mineralization is called subsequential. However, the mineralization of the outer limiting layer closely follows the secretion of a collagen-free organic matrix and is thus different from the mineralization of the internal layer in which the calcification front remains remote from the collagen matrix surface and corresponds to a delayed mineralization process. The isolated calcifications (Mandl's corpuscles) which develop in the unmineralized laminae of the internal layer are mineralized in the absence of matrix vesicles and without making contact with a pre-existing calcified tissue, probably by a heterogeneous nucleation of the collagen fibrils.
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    Keywords: Pancreatic endocrine cells ; Enteroendocrine cells ; Ultrastructure ; Cyprinidae
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    Notes: Summary The pancreatic endocrine cells of Barbus conchonius are concentrated in a large (principal) islet, located near the gall bladder, and in a number of smaller islets. Five types of endocrine cells can be distinguished in these pancreatic islets: B cells, A1 (or D cells), 2 types of A2 cells (A2r cells with round granules; and A2fl cells with flocculent granules) and a scarce 5th cell type. The hormones produced by B and A2fl cells are probably insulin and glucagon respectively. The A2r cell contains granules with the same diameter as the granules of the enteroendocrine type III cell of the gut. Both cell types may resemble the enteroglucagon-producing EG cell of mammals. The function of the A1 cells, which are frequently found without secretory granules, and of the 5th cell type, will be discussed. The pancreatic islets of B. conchonius are strongly innervated, which suggests the presence of a direct nervous control system. Some intermediate or mixed cells containing exocrine and endocrine A2r granules are found contiguous with the principal islet. The origin of pancreatic endocrine cells is also the subject of discussion.
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    Cell & tissue research 199 (1979), S. 249-256 
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    Keywords: Hepatocytes ; Birds ; Ultrastructure ; Estrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Administration of estradiol-17-β induces the synthesis of vitellogenin in primary cultures of chick embryo liver. The ultrastructural changes accompanying steroid induced vitellogenin synthesis were investigated in hepatocytes cultures incubated for 0, 12 and 24 h following hormonal treatment. Both electron microscopy and immuno-chemical techniques were used. The immuno-fluorescence data indicate that the cultures contain a cell population of 90–95% hepatocytes, both in control and estradiol-treated groups. Ultrastructurally, cultured hepatocytes are similar to in vivo ones, except for a reduced accumulation of storage materials, glycogen and lipids. In the estradiol-treated cultures hepatocytes show an accumulation of free ribosomes, enlargement of rough endoplasmic reticulum, and development of the Golgi apparatus. The data are discussed in relation to previous biochemical findings.
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    Cell & tissue research 201 (1979), S. 479-486 
    ISSN: 1432-0878
    Keywords: Ultrastructure ; Cercaria ; Striated tail muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The electron microscopic study of the tail of Cercaria chackai reveals that it contains four sets of striated muscle bundles located central to the nonstriated circular and longitudinal muscles. The striated muscle consists of longitudinally oriented lamellar myofibres. Each myofibre contains a single “U” shaped myofibril. The banding pattern is analogous to that of vertebrate striated muscle. The sarcolemma is a simple surface membrane. There are no transverse tubular extensions of sarcolemma. The sarcoplasmic reticulum (SR) is very well developed with cisternae, tubules, and vesicles. SR cisternae form dyadic couplings with the sarcolemma. There is a set of flattened tubules of SR origin traversing the myofibril exactly at the Z region. These tubules are unique to the striated muscle of the cercarian tail and may have functional significance. A diagrammatic reconstruction of the myofibre is presented.
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  • 57
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    Cell & tissue research 202 (1979), S. 133-143 
    ISSN: 1432-0878
    Keywords: Oogenesis ; Yolk formation ; Nurse cells ; Ultrastructure ; Diplura
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The egg chamber of Campodea consists of a group of nurse cells and an oocyte, and is surrounded by a simple, markedly flattened follicular epithelium. Three types of yolk occur in the oocytes: type I appears within elements of the rough endoplasmic reticulum; type II is produced by specific complexes of endoplasmic reticulum and dictyosomes; type III is incorporated by micropinocytosis. Histochemical tests show that mature yolk spheres contain proteins and polysaccharides. The main function of the nurse cells is to synthesize RNA, but they also produce small amounts of type I yolk. Phylogenetic conclusions are drawn from this and other studies of oogenesis in apterygote insects.
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  • 58
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    Cell & tissue research 202 (1979), S. 145-153 
    ISSN: 1432-0878
    Keywords: Oogenesis ; Yolk formation ; Ultrastructure ; Symphyla
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The paired ovaries of Symphyla are sac-shaped. Their interior is filled with synchronously developing oocytes surrounded by a simple follicular epithelium. Previtellogenic oocytes have spherical or oval nuclei, the latter containing a large number of small nucleoli. Part of the nuclear surface is covered by a thick “coat” made up of a granular substance of medium electron density. The “coat” is penetrated by cisternae of the smooth endoplasmic reticulum. Micropinocytosis is the principal source of yolk in Symphyla; a small amount of yolk material arises within the mitochondrial cristae. Vitellogenic oocytes are characterized by a highly active rough endoplasmic reticulum synthesizing a material, probably lipoprotein in nature, which is incorporated into lipid droplets.
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  • 59
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    Cell & tissue research 202 (1979), S. 431-438 
    ISSN: 1432-0878
    Keywords: Sturgeon ; Heart ; Ultrastructure ; Sarcoplasmic reticulum ; Granules
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    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of atrial and ventricular myocardial cells from Acipenser stellatus is described. The cells of the atrium are more loosely connected than those of the ventricle. Cell contact is by simple intercalated discs and by desmosomes. The cells are flattened, with peripheral myofibrils and a central region of mitochondria and the nucleus. The sarcoplasmic reticulum consists of subsarcolemmal tubules, that frequently extend towards the central mitochondria. Dyads are small and positioned at any sarcomeric level. No T-tubules are present. Specific granules are restricted to the atrial cell, and are sometimes present within the SR tubules.
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  • 60
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    Cell & tissue research 202 (1979), S. 453-460 
    ISSN: 1432-0878
    Keywords: Collagen ; Nerves ; Ganglia ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Nerves and ganglia from a variety of fish, amphibian, reptilian and mammalian species were studied by optical and electron microscopy. Observations using the Picrosirius-polarization method strongly suggest that two different types of collagen fibers are present in the connective tissues of nerves and ganglia. Electron microscopy of nerves and ganglia showed the presence of two different collagen fibril populations, distinguishable on the basis of diameter, located in different compartments of these structures. Thicker fibrils are present in nerve and ganglionic epineurium. Thinner fibrils are present in the endoneurium, surrounding nerve fibers and ganglionic cells, and between the concentric layers of perineurial cells. These results were consistently observed in all species studied and very probably represent a general phenomenon in vertebrates.
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  • 61
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Zona fasciculata ; Ultrastructure ; Stereology ; β 1–24-corticotropin
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    Notes: Summary Adult rats were given 15 daily subcutaneous injections either of synthetic β 1–24-corticotropin or of the corresponding placebo (controls) and were sacrificed 1 h after the final injection. In stimulated animals, the adrenal glands were increased in weight as compared to those of controls. Stereological analysis at light microscopic level of the outer zona fasciculata cells showed moderate volumetric increases of nuclei, cytoplasm and capillaries and a marked volumetric increase of lipid droplets in stimulated animals. Stereologic analysis of electron micrographs confirmed the marked increase in relative volume and surface density of lipid droplets, while volume fractions alone were increased for the Golgi apparatus and decreased for the endoplasmic reticulum and mitochondria. Biochemical analysis of the whole adrenal gland showed that the corticotropin injections produced a moderate increase in protein concentration, a marked increase in triglycerides and no appreciable changes in either phospholipid or cholesterol concentrations. The synthetic polypeptide therefore appears to have stimulating trophic effects on adrenal cortical cells, as shown by the increase in protein and cell size. However, it depresses the activity of the two types of organelle, endoplasmic reticulum and mitochondria, which have a major functional role in steroid synthesis. The increase of lipid droplets was interpreted as being primarily due to neutral fat accumulation, and secondarily to a diminished utilization of cholesterol for steroid synthesis. These findings suggest that, using this regime of administration, synthetic β 1–24 corticotropin, unlike native ACTH, inhibits steroid synthesis.
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  • 62
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    Keywords: HRP injection ; Lymnaea Stagnalis ; Neurohaemal areas ; Neurosecretory cell ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary The paired, electrotonically coupled neurosecretory Canopy Cells (CC) of the pond snail Lymnaea stagnalis were microiontophoretically injected with horseradish peroxidase (HRP). Whole mount preparations and ultrathin sections of injected CC were studied to describe in detail the morphology of the CC, their axon tracts and neurohaemal areas. The CC release their secretory product at three different sites, viz. from the soma and from axon terminals in the intercerebral commissure and in the median lip nerve. Neural control over the CC occurs by few synapses found exclusively on the CC axon, not on the cell body. One type of “en passant” synapse was identified. Two morphologically distinct types of synapselike structures (SLS) are numerous. The site of electrotonic coupling between the two CC is most probably located in the cerebral commissure. Serial sectioning showed that the axons contact each other over more than 130 μm. The contact is very intimate and the axon membranes interdigitate in a complex manner. Gap junctions, which are often described as the sites of electrotonic coupling, were not found.
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    Cell & tissue research 202 (1979), S. 33-39 
    ISSN: 1432-0878
    Keywords: Gregarina ; Ultrastructure ; Gram negative bacteria
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    Topics: Biology , Medicine
    Notes: Summary An investigation of bacteria-like structures, which are found in large numbers in the endoplasm of the eugregarine, Gregarina garnhami, was carried out using light and electron microscopy. Gram staining indicates that these structures are gram negative; they show orange fluorescence when stained with acridine orange. Ultrastructural observations show that they resemble the endosymbiotic bacteria found in other protozoan species. Some of these structures appear to be dividing, and the possibility that these structures are in fact bacteria is discussed.
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    Protoplasma 100 (1979), S. 73-83 
    ISSN: 1615-6102
    Keywords: Bivalve ; Fertilization ; Laternula limicola ; Sperm-egg interaction ; Temporary-acrosome ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ultrastructural studies on sperm-egg interaction at the time of fertilization inLaternula limicola were performed. The “temporary-acrosome” did not change morphologically while the sperm passed through the egg investments. At the onset of sperm entrance into the egg, however, the temporary-acrosome and mitochondria were eliminated from the sperm. Afterwards the sperm was engulfed by the egg surface without membrane fusion of the gametes. After entry the sperm nucleus was surrounded by four membranes: the plasma membranes of the egg and of the sperm, and the membranes of the sperm nuclear envelope. As the sperm nucleus differentiated into the male pronucleus, the plasma membranes of both the sperm and egg were initially vesiculated, then dispersed into the egg cytoplasm. Finally, the sperm nuclear envelope changed into the male pronuclear membrane accompanying sperm chromatin dispersion.
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  • 65
    ISSN: 1615-6102
    Keywords: Ciliata ; Crystals ; Euplotes ; Lithosomes ; Microanalysis ; Ultrastructure
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    Topics: Biology
    Notes: Summary In the cytoplasm of the marine ciliateEuplotes vannus, there exist two conspicuous types of membrane bound inclusions: 1. irregularly shaped crystals which are highly anisotropic; 2. globular lithosomes characterized by concentrically arranged layers of deposits which exhibit only faint birefringence. Normally, both structures form distinct accumulations. Energy dispersive X-ray microanalysis of these accumulations reveals a high content of calcium and phosphorus, besides magnesium, sulphur and chlorine. Analysis of cell areas devoid of the inclusions show significantly lower calcium- and phosphorus-peaks.
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  • 66
    ISSN: 1615-6102
    Keywords: Mating ; Sporidia ; Tilletia ; Ultrastructure ; Wheat bunt
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    Topics: Biology
    Notes: Summary Primary sporidia ofTilletia caries (DC.) Tul. are borne on denticles at the tips of promycelia. The promycelia contain many small vacuoles and mitochondria and numerous lipid bodies. As the primary sporidia develop, the promycelial cytoplasm passes into the nascent cells. Septa develop between the bases of mature sporidia and the tips of the denticles. Sporidia that abscise from the denticles commonly have prominent birth scars at their bases. The sporidia have very thin walls, few vacuoles, attenuated mitochondria, and numerous lipid bodies. Conjugation pegs are generally produced by both members of a conjugating pair of sporidia and there are bud scars where they emerge from the sporidia. The sporidial walls are apparently hydrolyzed during emergence of the pegs. Vesicles are sometimes present at the tips of the conjugation pegs and, before fusion, electron-dense accumulations are sometimes observed between the tips of adjacent pegs. The approaching conjugation pegs are precisely aligned prior to fusion, suggesting polar communication. The walls of the conjugation pegs fuse and then are hydrolyzed. Fused sporidia are relatively homogeneous in content. The nucleus in a sporidum is often close to the conjugation tube and occasionally is partly within the fusion tube.
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  • 67
    ISSN: 1615-6102
    Keywords: Amoeba proteus ; Anaerobiosis ; Metabolic inhibitors ; Mitochondria ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Anaerobiosis or exposure to the metabolic inhibitors potassium cyanide and Antimycin A produced changes in the form of living amoebae. These were accompanied by mitochondrial changes in fixed cells. Increasing the anaerobic period increased the percentage of mitochondria affected and resulted in a gradual reduction and eventual loss of the condensed Type I mitochondria ofAmoeba proteus. The rounder Type II mitochondria were not lost but underwent varying degrees of disruption, vesiculation of the cristae being evident after 5 hours exposures and matrical inclusions after 18 hours exposures. Similar cristal vesiculation was seen after 30 minutes treatments with potassium cyanide. Providing treatments were terminated before cell viability was lost, all mitochondrial abnormalities were reversible on return to normal culturing conditions. The longer the period of anaerobiosis the longer was the recovery time required for the return of normal mitochondrial structure and the re-equilibration of control Type I to Type II mitochondrial frequencies. The relationship between mitochondrial conformation and functional integrity is discussed in the light of these findings.
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    Cell & tissue research 197 (1979), S. 165-167 
    ISSN: 1432-0878
    Keywords: Ultrastructure ; Chick embryo ; Muscular dystrophy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary New Hampshire chickens, homozygous for inherited muscular dystrophy, display clinical manifestations at an early age. A fine structural examination of embryos from this strain shows marked degenerative changes four days prior to hatching. The Z bands appear to dissolve progressively to the point where finally the myofibrils become uniformly dense with no detectable banding patterns.
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    Cell & tissue research 197 (1979), S. 113-135 
    ISSN: 1432-0878
    Keywords: Domestic fowl ; Ultimobranchial gland ; Parathyroid gland ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the polymorphic vesicular component of the ultimobranchial gland of the domestic fowl (Gallus gallus domesticus) has been described in detail, together with the structure of the cell strands interconnecting the vesicles and the parathyroid nodules lying within the ultimobranchial stroma. The vesicles frequently appear to arise from the nodules by way of the cell strands. The strands show a structure of their component cells intermediate between that of the parathyroid and the vesicular cells, although the position at which the strand changes from an essentially parathyroid structure to an essentially vesicular structure is very variable. The degree and kind of secretory activity within different cell types has been described. A review of the structure of ultimobranchial glands throughout the vertebrates shows that similar tissue with a similar secretory potential has been observed in all vertebrate classes, suggesting a functional significance for this part of the gland.
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    Cell & tissue research 196 (1979), S. 531-539 
    ISSN: 1432-0878
    Keywords: Fiber types ; Red muscle ; Semitendinosus muscle ; Ultrastructure ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The semitendinosus muscle of the rabbit is composed of a homogeneous fiber population. Fiber typing was carried out by means of light and electron microscopy according to which this muscle exhibits structural features that are either characteristic for both ‘red’ and ‘white’ fibers, or that do not allow for any classification according to the A-, B-, C-fiber system. Hence the long-held assumption that the semitendinosus muscle of the rabbit represents a classical paradigm of a ‘red’ muscle should be revised in light of the present results.
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    Cell & tissue research 197 (1979), S. 39-59 
    ISSN: 1432-0878
    Keywords: Lamina ganglionaris ; First, second order neurons ; Neuroanatomy ; Ultrastructure ; Hemipterans (Notonecta glauca, Corixa punctata, Gerris lacustris)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neuronal elements, i.e. first and second order neurons, of the first optic ganglion of three waterbugs, N. glauca, C. punctata and G. lacustris, are analyzed on the basis of light and electron microscopy. Eight retinula cell axons, leaving each ommatidium, disperse to different cartridges as they enter the laminar outer plexiform layer. Such a pattern of divergence is one of the conditions for neuronal superposition; it is observed for all three species of waterbugs. The manner in which the receptors of a single bundle of ommatidia split of within the lamina, whereby information from receptors up to three or five horizontal rows away can converge upon the same cartridge, differs among the species. Six of the eight axons of retinula cells R1-6, the short visual fibers end at different levels within the bilayered lamina, whereas the central pair of retinula cells R7/8, the long visual fibers, run directly through the lamina to a corresponding unit of the medulla. Four types of monopolar cells L1–L4 are classified; their branching patterns seem to be correlated to the splitting and termination of retinula cell axons. The topographical relationship and synaptic organization between retinula cell terminals and monopolar cells in the two laminar layers are identified by examination of serial ultrathin sections of single Golgi-stained neurons. An attempt is made to correlate some anatomical findings, especially the neuronal superposition, to results from physiological investigations on the hemipteran retina.
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    Cell & tissue research 197 (1979), S. 105-112 
    ISSN: 1432-0878
    Keywords: Thymus ; Myoid cells ; Histochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histochemical and ultrastructural properties of myoid cells in the thymus of the frog were investigated and compared with properties of skeletal muscle fibres. The histochemical reactions of phospholipids, phosphorylase, succinic dehydrogenase and adenosine triphosphatase activities in myoid cells were characterized by considerable variability. Individual myoid cells apparently possess different enzyme activities which correspond to different stages of development, maturity and degeneration of these cells. The mature mononucleated myoid cells have similar enzymatic properties to the fast muscle fibres of the frog. This finding has been extended by ultrastructural observations. Features, typical of fast muscle fibres of the frog, e.g. the presence of the M-line, straight and narrow Z-line and well developed triads were found in the majority of mature myoid cells.
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    Cell & tissue research 203 (1979), S. 311-320 
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Acipenseridae ; Ultrastructure ; Quantitative analysis ; Spawning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The percentage of peptidergic (A1 and A2) and adrenergic (B) neurosecretory terminals was studied in the neurohypophysis of sexually mature female sturgeons. Neurosecretory terminals of the A2 type prevail in the neurohypophysis, whereas A1 and B terminals are rare. The activity of these types of terminals was established (1) during upstream migration, (2) shortly after spawning, and (3) three to six weeks after spawning. Terminals of B type are the most active elements during all the periods studied. These elements become strongly activated in sturgeons during upstream migration, i.e., earlier than the peptidergic neurosecretory terminals. Peptidergic terminals, especially elements of type A2, become synchronously and strongly activated in fish shortly after spawning. In the late postspawning period neurosecretory terminals of all three types become synchronously inactive, persisting in a quiescent state in comparison to the two previous periods. The appearance of neurosecretory material discharged into the intercellular clefts by exocytosis correlates on the whole with the activity level of the A1 and A2 terminals in each individual studied. A functional correlation exists between the activity of the peptidergic and adrenergic neurosecretory terminals in the neurohypophysis. The data obtained are discussed with reference to a concept regarding spawning in some fish species as a physiological stress (Polenov et al., 1976). A possible dual control (peptide and monoamine neurohormones) over the function of visceral organs and glandular cells of the intermediate lobe of the hypophysis is also suggested (Polenov, 1970, 1975, 1978; Polenov and Belenky, 1973).
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    Cell & tissue research 203 (1979), S. 393-401 
    ISSN: 1432-0878
    Keywords: Guinea pig ; Hypothalamus ; Differentiation ; Organ culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of neurophysin-oxytocin and neurophysinvasopressin containing neurons of the guinea pig was studied in vitro. Supraoptic (SO) and paraventricular (PV) nuclei were explanted from guinea pig foetuses at the 40th day of gestation and cultured in Maximov slides for nearly fifty days. The cultures were observed daily under a phase-contrast microscope. Explants were fixed every five days for observation with the electron microscope. At the time of explantation, magnocellular neurons were still immature. They acquired the morphologic characteristics of mature neurons, with axosomatic synapses, after about 10 days in vitro. After 15–20 days in vitro, they contained in addition neurosecretory granules (NSG), first in the Golgi region, then also dispersed in the cytoplasm. In the oldest culture (45–50 days), signs of granulolysis were regularly found. It appears that magnocellular neurosecretory neurons are able to differentiate in vitro from a primitive state in the absence of specific stimulation.
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  • 75
    ISSN: 1432-0878
    Keywords: Neurohemal organ ; Neurosecretion ; Moulting gland ; Ultrastructure ; Crustaceans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Chez Sphaeroma serratum, la mue de puberté est suivie d'une dégénérescence de l'organe Y (glands de mue). Le plexus nerveux céphalique latéral, organe neurohémal accolé à cette glande a été l'objet de la présente étude ultrastructurale. Cet organe représente un centre de stockage de neurosécrétions qui proviennent d'une part, de deux cellules autochtones (cellules plexales) situées au sein même de ce plexus, d'autre part, de cellules neurosécrétrices situées dans le “ganglion mandibulaire” (cellules de type A). Chez les individus pubères, les cellules plexales et les cellules A du ganglion sous-oesophagien synthétisent des granules de neurosécrétion dont la taille est respectivement 1550±50Å et 1570±40Å. Il a été reconnu au sein du plexus 5 catégories de terminaisons dont les granules proviendraient pour deux d'entre elles des cellules plexales et des cellules A du “ganglion mandibulaire”. Chez les animaux pubères on observe un arrêt de la synthèse des granules de neurosécrétion au sein des cellules plexales et des cellules A du “ganglion mandibulaire”. Simultanément on enregistre dans le plexus la raréfaction puis la disparition des divers types de granules. Ce processus atteint en premier les terminaisons correspondant aux cellules plexales et aux cellules A du “ganglion mandibulaire”. La dégénérescence de la glande de mue chez les mâles pourrait être en relation avec l'arrêt de synthèse de ces cellules.
    Notes: Summary The present ultrastructural study deals with the lateral cephalic nerve plexus of Sphaeroma serratum, a neurohemal organ joined to the Y organ (ecdysial gland). This plexus acts as a storage centre for neurosecretory products from two sources: the two autochtonous cells (plexus cells) within the plexus itself, and the neurosecretory cells in various parts of the central nervous system, particulary the “mandibular ganglion” (A-cells). In prepuberal animals, plexus cells and subesophageal A-cells produce neurosecretory granules of two types measuring 1550±50Å and 1570±40Å respectively. Five categories of axon terminals were distinguished in the plexus. The granules found in two of these terminal types are believed to come from the plexus cells and from the “mandibular ganglion” A-cells. Cessation of production of neurosecretory granules in these A cells and plexus cells was observed in puberal animals, in the plexus with concomitant depletion and disappearance of different granule categories. The first axon terminals affected by this process are the two categories containing granules originating in the plexus and “mandibular ganglion” A-cells. Degeneration of the ecdysial gland in male Sphaeroma serratum might be connected with the cessation of granule formation in these two types of cell.
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    Cell & tissue research 203 (1979), S. 427-442 
    ISSN: 1432-0878
    Keywords: Aphids ; Sensilla ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An electron microscopical study was made of the coeloconic and placoid sensilla on the antennae of the aphids Aphis pomi, Macrosiphum euphorbiae, Nasonovia ribis-nigri, and Pemphigus bursarius. Scanning electron microscopy revealed some variation in morphology which may be functionally important but is more likely to reflect the evolution of these species. The placoid sensilla were shown by transmission electron microscopy to have the same basic structural pattern. Each group of two or three neurons is surrounded by two ensheathing cells. The ciliary regions of the dendrites pass through a vacuole into a cavity between an outer and an inner cuticle where they may be connected to the dendritic branches although such connections were not seen. Small pores (8 nm diameter) partially penetrate the cuticle implying that these sensilla have an olfactory function. They are suggested to be important in host selection by alate aphids. The coeloconic sensilla are poreless pegs with nonsensory cuticular projections at their tips. The distal portions of their dendrites contain densely packed microtubules and the cellular arrangement of the sensilla is similar to that of the placoid sensilla. It is suggested that they may function as thermoreceptors.
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    Cell & tissue research 203 (1979), S. 503-506 
    ISSN: 1432-0878
    Keywords: Muscle ; Disuse atrophy ; Insects ; Ultrastructure ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The changes which occur in the ultrastructure of the mesothoracic retractor unguis muscle of the cockroach Periplaneta americana as a result of disuse are described. Breakdown of myofibrils, sarcoplasmic reticulum, and mitochondria are all marked, this degeneration only being apparent 9 weeks after the operation.
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    Cell & tissue research 203 (1979), S. 499-502 
    ISSN: 1432-0878
    Keywords: Special vesicles ; Membrane transport ; Gastropoda ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary The adventitial cells surrounding the spermatheca of the reproductive system of Sonorella santaritana (Mollusca: Gastropoda) appear to have an unusual system of vesicles. Electron micrographs of the membranes forming these vesicles show that they have multiple openings to the cell's exterior and that each opening has a “pore complex”. In addition, secondary vesicles appear to be generated by the primary vesicles. Evidence is presented suggesting that these vesicles represent a previously unreported membrane transport system.
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    Cell & tissue research 204 (1979), S. 95-109 
    ISSN: 1432-0878
    Keywords: Pineal gland ; Gerbil ; Pinealocytes ; Morphometric analysis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By means of morphometric analytical procedures, a diurnal rhythm in the cellular volume of gerbil pinealocytes was determined. This rhythm has been attributed primarily to a change in the cytoplasmic volume of the pinealocytes which is low during the daylight hours and increases to reach a peak during the middle of the dark period. At the ultrastructural level, six cytoplasmic components of the pinealocytes were found to exhibit a rhythm: free cytoplasm, smooth endoplasmic reticulum (SER), rough endoplasmic reticulum (RER) and ribosomes, secretory vesicles, microtubules, and mitochondria. The presumptive secretory vesicles and the microtubules reached a peak in volume one hour before lights-off. It is suggested that lights-on and lights-off both signal a decrease in size and/or number of the secretory vesicles. The SER and RER/ribosomes reached their peak volume one hour after lights-off which is interpreted as indicating a peak in indoleamine synthesis and protein synthesis, respectively. The volume of free cytoplasm exhibits two peaks; one occurs one hour before lights-off while the second peak occurs in the middle of the dark phase. It is suggested that, although part of the secretory product of the pinealocyte may be present in dense-cored vesicles, other locations could include the free cytoplasm and clear secretory vesicles.
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    Cell & tissue research 204 (1979), S. 233-241 
    ISSN: 1432-0878
    Keywords: Merkel cell ; Sensory receptors ; Epidermis ; Frog tadpoles ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopy of larval tentacles of Xenopus laevis tadpoles revealed that they are richly innervated structures containing a high concentration of neurite complexes with epidermal Merkel cells. Myelinated sensory nerve fibres supplying each tentacle are abundant. Prior to penetrating the epidermis, they gradually shed their myelin sheaths and Schwann-cell coverings, and subsequently end as naked axon terminals within the epidermis. Many of these intra-epidermal neurites terminate in intimate synaptic contact with granulated Merkel cells. Groups of dense-cored vesicles characterize the Merkel cell, while clusters of clear synaptic vesicles occupy the adjacent sensory nerve terminals. At sites of synaptic contact, the two cells often exhibit an increased thickening of their apposing membranes. Such Merkel cell — sensory neurite complexes occur as isolated units and are profusely scattered throughout the extent of each tentacle. Their ultrastructure is, in most respects, similar to that described previously in specialized regions of other vertebrate and amphibian species. It is suggested that they represent the morphological basis for tactile sensitivity and impart a mechanoreceptive function to the larval tentacle prior to metamorphosis. The larval tentacle in this anuran species may represent a new and convenient research model for future developmental and experimental studies of trophic interactions between sensory nerve fibres and Merkel cells.
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  • 81
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    Keywords: Pineal gland ; Ultrastructure ; Cotton rat (Sigmodon hispidus)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fine structural features of pinealocytes of cotton rats (Sigmodon hispidus) were examined. Golgi complexes, mitochondria, endoplasmic reticulum and polysomes are usual organelles seen in the perikaryonal cytoplasm of pinealocytes. Many non-granulated vesicles (40 to 80 nm in diameter) and a few granulated vesicles (about 100 nm in diameter) are associated with the Golgi cisternae. Occasionally, the cisternae contain granular materials. The perikaryonal cytoplasm of pinealocytes is characterized by the presence of inclusion bodies. These bodies are usually round in shape, not bounded by a limiting membrane and composed of fine granular or filamentous materials of high electron-opacity, which are similar in appearance to the substance seen in the nucleolonema. Pinealocyte processes, filled with abundant non-granulated vesicles and some granulated vesicles, are mainly found within the parenchyma and occasionally in perivascular spaces.
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  • 82
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    Cell & tissue research 196 (1979), S. 449-454 
    ISSN: 1432-0878
    Keywords: Gastric epithelium (Rat) ; Phagocytosis ; Cell loss ; Aspirin ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Gastric surface epithelial cells (SEC) from fed rats, from rats fasted for 16 h and from mucosae exposed in an ex-vivo chamber to 16 mM aspirin for 5 min were examined by transmission electron microscopy. SEC have the capability to phagocytose adjacent epithelial cells and parietal cells. Phagocytosis is rare in mucosae from fasted animals but common in fed animals or after brief exposure to aspirin. Phagocytic capabilities are not restricted to the progenitor zone but exist throughout the surface epithelium. Phagocytosis may provide a mechanism for the removal of damaged or senescent cells from the surface epithelium.
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  • 83
    ISSN: 1432-0878
    Keywords: Interrenal tissue (birds) ; Ultrastructure ; Corticotropin ; Adenohypophysectomy ; Morphometry
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    Topics: Biology , Medicine
    Notes: Summary Adrenal glands from ACTH-treated intact ducks and chronically adenohypophysectomized ducks showed clear zonation into a subcapsular zone (SCZ) and an inner zone (IZ). Adenohypophysectomy caused ultrastructural changes in the IZ but not in the SCZ cells. These included increases in lipid droplets, changes in mitochondrial cristae from tubular to shelf-like, and changes in the shape of the nuclei from spherical to crenated. These changes were reversed by treatment with ACTH. Also, cells of the IZ, but not the SCZ, of adrenals from intact birds given ACTH showed more SER, more dense bodies, fewer lipid droplets and more prominent Golgi complexes. IZ cells incubated in buffer containing no ACTH developed mitochondria with shelf-like cristae and numerous opaque granules in the matrix. Exposure to buffer containing ACTH caused the mitochondrial cristae to become tubular and the matrix granules either decreased in number or disappeared. The granules could be extracted by incubating sections with chelating agents. The mitochondria in SCZ cells did not respond structurally to the presence of ACTH in the incubation medium but the matrix granules, like those in IZ cells, responded to the presence of chelating agents.
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  • 84
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    Cell & tissue research 196 (1979), S. 511-518 
    ISSN: 1432-0878
    Keywords: Rabbit ; Aortic body ; Chemoreceptors ; Stereology ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The chief cells of the aortic body (subclavian body) of adult New Zealand white rabbits were examined by ultrastructural stereological analysis. The chief cell nuclei occupy 26.5% of the total volume. Dense-core vesicles account for 16.5% of the cytoplasmic volume, followed by mitochondria (11.6%), endoplasmic reticulum (3.3%), and Golgi apparatus (0.6%). The dense-core vesicles measure approximately 131.6nm in diameter (corrected) and exhibit a heterogeneous size distribution. Both perivascular adrenergic nerve terminals and presumptive afferent terminals presynaptic to the chief cells are observed. The mean synaptic vesicle size of the terminals adjacent to chief cells is 54 nm. The heterogeneous size distribution of the dense-core vesicles of chief cells may indicate the storage of different biogenic amines and/or different secretion or maturation states within the chief cells.
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  • 85
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    Cell & tissue research 197 (1979), S. 413-419 
    ISSN: 1432-0878
    Keywords: Luteal cells ; Secretory granules ; Sheep ; Ultrastructure
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    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the corpus luteum of the sheep has been examined at the mid-stage of the estrous cycle when progesterone secretion is active. Secretory granules are associated with the secretion of this hormone, and the evidence indicates that the granule membrane becomes incorporated into the plasma membrane during exocytosis. Further evidence of this process has been obtained from studies on the uptake of horseradish peroxidase by the luteal cells.
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  • 86
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    Cell & tissue research 198 (1979), S. 105-117 
    ISSN: 1432-0878
    Keywords: Swim bladder ; Physostome ; Cyprinid ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The swim bladder of the cyprinid Carassius auratus (goldfish) is a two-chambered organ connected to the esophagus by a pneumatic duct. The anterior chamber is lined by a single type of squamous epithelial cell. Two types of epithelial cells are present in the posterior chamber. Flattened cells with differences in the electron density of the cytoplasm line most of the chamber. Darker cells generally contain large amounts of glycogen. Cuboidal epithelial cells also occur in the posterior chamber. A glandular layer external to the muscularis in the posterior chamber is composed of large cells containing little glycogen, an extensive Golgi apparatus, and numerous mitochondria with single large granules. Capillaries and nerves are present in large numbers in this layer. Blood vessels form micro-retia mirabilia in the submuscular layer external to the glandular layer. Vessels are of two distinct types with wide lumina and flattened endothelium characterizing the venous vessels. Arterial vessels have smaller lumina, thick endothelial cells with prominent pinocytotic vesicles, and surrounding pericytes. Collagen is present in three forms in this swim bladder — large tactoids in the tunica externa of the anterior chamber, smaller tactoids in the lamina propria of the posterior chamber, and small fibrils in all other areas.
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  • 87
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    Cell & tissue research 198 (1979), S. 191-199 
    ISSN: 1432-0878
    Keywords: Spermatogonia ; Man ; Stem cells ; Morphology ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present investigation is concerned with establishing morphological criteria of spermatogonial stem cells in man. Testicular biopsies from patients having undergone semicastration for malignant tumors and radioand chemotherapy for one year following the operation were studied light and electron microscopically. Those spermatogonial types that survived the treatment were regarded as stem cells in view of the fact that the stem cells, in contrast to the more differentiated spermatogonia, are radiation resistant and less sensitive to various noxious agents. In 7 out of 28 cases examined, a small number of spermatogonia was found adjacent to the basement membrane. The majority of these cells show the characteristic features of pale type A spermatogonia, while a few cells may represent variants of this cell type. The dark type A spermatogonia are almost completely eliminated from the seminiferous tubules. A concept is proposed that the stem cells of the human testis may be derived from the pale type A spermatogonia or the variants of this cell type.
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  • 88
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    Cell & tissue research 196 (1979), S. 39-57 
    ISSN: 1432-0878
    Keywords: Midgut (Collembola) ; Ultrastructure ; Cytochemistry ; Renewal ; Ageing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The midgut cells of Tomocerus minor (Insecta, Collembola) were examined with the electron microscope and cytochemically. The midgut epithelium consists of a series of cells characterised by numerous mineral concretions scattered throughout the cytoplasm. Mitochondria are abundant; microvilli are well developed at the apical surface of the cell. A zonula continua (continuous junction) characterises the apical contact region of these cells. Polysaccharides, glycoproteins and carbohydrate components have been demonstrated on the surface of microvilli. Peritrophic membranes surround the food bolus and preserve midgut cells from mechanical abrasion. Lysosomes are present during the alimentary period and show strong acid phosphatase activity. During an intermoulting cycle, two stages can be observed: (1) the postexuvial feeding period during which cytoplasmic extrusions appear at the apical part of the cell: lysosomes increase in number and autophagic vacuoles appear. (2) The preexuvial fasting period; a new epithelium grows beneath the old one and pushes it into the lumen. Degeneration processes can be observed in the old epithelium. This excretory reactivity of the midgut epithelium has been compared to the cycle of the cuticle.
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  • 89
    ISSN: 1432-0878
    Keywords: Liver ; Goldfish ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A re-examination of goldfish liver was made through the use of SEM of fractured samples and TEM of ultrathin-sections and freeze-etch replicas. Several new hepatic fine structures described in the present study are morphologically similar to those reported previously in many higher vertebrates including mammals. Hepatic sinusoids of goldfish contain fenestrations which are arranged into sieve plates. Although the hepatic plates are made up of two layers of hepatocytes, the parenchymal cells of goldfish liver are morphologically similar to mammalian hepatocytes, particularly with respect to the sinusoidal surfaces which are studded with numerous microvilli. The intercellular surfaces of hepatocytes have both nexus and desmosomal junctions, similar to those found in various epithelial cells of higher vertebrates, as cell attachments and communication foci. Tight junctions are found mainly between the openings of the intracellular bile canaliculi and the intralobular bile ductules which are situated in the center of the bicellular hepatic plate.
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  • 90
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    Cell & tissue research 196 (1979), S. 289-306 
    ISSN: 1432-0878
    Keywords: Spleen ; Carp ; Sheathed capillaries ; Macrophages ; Histochemistry ; Ultrastructure
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    Topics: Biology , Medicine
    Notes: Summary In the spleen of the carp arterial capillaries of a highly differentiated structure have been studied by light and electron microscopy. These capillaries share various structural characteristics with the sheathed capillaries (ellipsoids of Schweigger-Seidel) of higher vertebrates. The long arterial capillaries of the carp spleen are provided with cuboidal endothelial cells containing filaments approximately 7 nm in diameter. There is no basal lamina. The endothelial cells form various types of cell junctions, but there are also extensive areas without any junctions. Here, a free passage is possible between the capillary lumen and the subendothelial space. The capillaries possess a single-layered sheath of macrophages. Characteristically, the sheath macrophages possess long and slender cell processes forming a loose framework, the meshes of which are filled with lymphocytes and spindle cells. The sheath macrophages show a zone of ectoplasm rich in filaments. They also contain numerous phagolysosomes rich in hydrolytic enzymes, as identified histochemically. The sheath is sharply limited against the pulp by a thick layer of collagen fibers.
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  • 91
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    Keywords: Adrenal medulla (rat) ; Vinblastine ; Ultrastructure ; Catecholamines ; Enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of a single high dose (10mg/kg) of vinblastine (vb) sulfate (“Velbe”, Lilly) on the ultrastructure, catecholamine (CA) content and activity of CA-synthesizing enzymes of the rat adrenal medulla were studied for up to 120h after intravenous injection of the drug. By 1 h, microtubules were virtually absent from chromaffin cells and preganglionic cholinergic axons, and typical paracrystals had appeared inside the nerve fibers. By 16h microtubules were completely reconstituted and paracrystals had disappeared. From 16h onwards, there was an increasing depletion of storage granules from adrenaline (A) — producing cells, which coincided with biochemical determinations showing a reduction of adrenal A to about 40 % of control levels by 48 h, with noradrenaline (NA) remaining in the range of controls. Both A- and NA-storing cells showed an extensive proliferation of the rough endoplasmic reticulum (ER). Vb caused a marked increase in tyrosine hydroxylase (TH; +113%) and dopamine β-hydroxylase (DBH; +82%) activities after 48 h. Splanchnicotomy completely abolished the vb-mediated increase in TH and DBH activities. A smaller increase (+ 47 %) in enzyme activity was observed with phenylethanolamine N-methyltransferase (PNMT). Vb (10−5M) had no apparent effect on granule content and the amount of rough ER in chromaffin cells, which were cultured for 48 h. The results demonstrate that a single high dose of vb has relatively little short-term effects on the rat adrenal medulla, but causes drastic long-term changes in CA-content and enzyme activities that are mediated by the preganglionic nerves. These changes could be interpreted as an effort to compensate for a loss of CA-stores in peripheral adrenergic nerves (cf. Cheney et al., 1973). The differential long-term effect of vb on adrenal NA and A might be due to the lower induction of PNMT as compared to TH and DBH activities and/or to a preferential release of A versus NA, which may occur at high frequencies of stimulation of the splanchnic nerves.
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  • 92
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    Cell & tissue research 196 (1979), S. 385-395 
    ISSN: 1432-0878
    Keywords: Uterus ; Endometrium ; Smooth muscle ; Culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Primary cultures initiated from normal human uterine endometrium after total enzymatic dissociation contained epithelioid cells and smooth muscle cells. The smooth muscle cells were subsequently isolated by differential trypsinization and grown in culture for 36 ± 4 generations. Ultrastructural examination of log and post-confluent cultures of cells at low and high population doubling levels revealed characteristics similar to those of published reports on other smooth muscle cells studied in vivo and in vitro. Among the common features present were: (a) abundant bundles of 60–70 Å myofilaments; (b) branched mitochondria; (c) stacks of cisternae of rough endoplasmic reticulum; (d) caveolae intracellulares; (e) nexuses. Other features included ovoid nuclei, a well developed Golgi apparatus and abundant free ribosomes. The subcultured cells exhibited features of dedifferentiation in the log phase of growth and at post-confluency. However, the post-confluent cells showed characteristics indicating redifferentiation back towards their in vivo morphology. Smooth muscle cells isolated from endometrial curettings may provide a useful model for biochemical and pharmacological studies of a cell type derived from a hormonal target tissue as the cells “age” in culture.
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  • 93
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    Cell & tissue research 196 (1979), S. 519-524 
    ISSN: 1432-0878
    Keywords: Y-organ ; Moulting gland ; Astacus ; Steroid production ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The electron microscopical investigation of Y-organs of Astacus astacus revealed that during intermoult (stage C) the cytoplasm is poorly developed and that it increases at premoult (stage D). It then shows the typical signs of steroid production, namely agranular endoplasmic reticulum and mitochondria of the tubular type. Furthermore, a larger type of mitochondria with a regular pattern of internal structure is described.
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  • 94
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    Calcified tissue international 25 (1978), S. 145-159 
    ISSN: 1432-0827
    Keywords: Bird egg shell ; Ultrastructure ; Calcification ; Electron diffraction ; Microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The egg-shell of Japanese quail was studied by several techniques. Semithin sections (1μm thick) of non-decalcified shell were observed by normal and polarized light microscopy. Thin sections of non-decalcified shell, examined by transmission electron microscopy, permitted us to observe the forms and dimensions of crystals of calcite within different layers of the shell: mammilary layer, layer of cones, palissade layer and surface crystal layer. There appears to be two distinct zones in the layer of cones as well as in the superficial crystal layer. Electron microdiffraction revealed the orientation of calcite crystals in the columns. Some crystal defects (twins?) were described and the possibility of their artefactual formation during ultramicrotomy is discussed. Localization of Ca, Mg, P and S were made by X-ray microanalysis of semithin sections. This technique shows that shell membranes, and chiefly the true cuticle, are also mineralized but, in these layers, minerals are not crystallized. Otherwise the distribution of Mg is not uniform throughout the shell thickness; it is less concentrated in the external zone of the layer of cones. These results together with observation of developing shells by scanning electron microscopy allowed us to propose a scheme for shell organization of the quail egg. This organization was related with decalcification which occurs during hatching.
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  • 95
    ISSN: 1432-041X
    Keywords: Ultrastructure ; Scanning cytophotometry ; Chromatin ; Chondrocytes ; Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les cellules cartilagineuses des membres postérieurs deTriturus cristatus en régénération après amputation, ont été étudiées en microscopie électronique et par cytophotométrie à balayage. Nous nous sommes intéressés à la structure et à la distribution de la chromatine mais aussi à différents organites cytoplasmiques. Dans l'étude de cytophotométrie à balayage, la chromatine a été considérée à travers son constituant majeur, l'ADN, coloré par la réaction de Feulgen. Au cours de la régénération du membre, l'hétérochromatine initialement condensée, essentiellement accolée à la membrane nucléaire se décondense. Les vacuoles du cytoplasme, caractéristiques des animaux âgés par rapport aux animaux jeunes, disparaissent, les mitochondries et le reticulum endoplasmique rugueux deviennent plus abondants. Les caractéristiques nucléaires de l'activation cellulaire apparaissent précocement, précédent les modifications cytoplasmiques et conduisent à des cellules en tous points identiques aux cellules d'animaux jeunes en dehors de tout processus régénératif. Cette phase d'euchromatisation et de restructuration cytoplasmique est peut-être nécessaire à l'accroissement d'activité métabolique et à la division cellulaire qui suivent. Son déroulement peut expliquer tout au moins le ralentissement de la régénération observé chez les animaux âgés par rapport aux animaux jeunes.
    Notes: Summary Cartilaginous cells of aged newts (Triturus cristatus) were studied during hind limb regeneration. The electron microscope was used to study the structure and distribution of chromatin in the cell nuclei, while the DNA content of the chromatin was measured by means of a scanning cytophotometer. Changes in the ultrastructure of the cytoplasm during regeneration were also studied. It was observed that the structure and distribution of chromatin in the activated cell is greatly modified. In the non-activated cell of the aged newt, the chromatin is found highly condensed and distributed peripherally close to the nuclear membrane. In contrast, in the activated cells, the chromatin is much less condensed and is distributed throughout the nucleus. Moreover, cytoplasmic vacuoles, found only in the non-activated aged cells, disappear and an increase in the mitochondria and rough endoplasmic reticulum is also observed. Changes in the nuclear structure are observed prior to the cytoplasmic modifications. It is interesting to note that the process of activation induces structural changes in the aged cells which make these cells appear to be structurally identical to the young cells. This process of rejuvenation takes 3–5 days in the newt. We suggest that these structural changes of the chromatin and cytoplasm in the aged cells are necessary to increase the metabolic activity which precedes cell division. It may also explain why regeneration takes a longer time in the aged animals than in the young ones.
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  • 96
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    Pure and applied geophysics 116 (1978), S. 1274-1283 
    ISSN: 1420-9136
    Keywords: Seismology ; Surface-wave dispersion ; Higher mode surface waves ; Italy, phase vel. of surface waves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract A new method is presented for the determination of higher mode dispersion over small distances, using two stations. From an intermediate focus earthquake it has been possible to measure the dispersion of the first and second Rayleigh modes in the period range 25–35 sec and 25–70 sec respectively. Using the almost linear array of Italian long-period stations TRI, BLZ, OLB an estimate of the average errors is also made.
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  • 97
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    Pure and applied geophysics 116 (1978), S. 1299-1306 
    ISSN: 1420-9136
    Keywords: Seismology ; Rayleigh wave dispersion ; Phase velocity of Rayleigh waves ; German Dem. Rep., Rayleigh waves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Summary The phase velocity curve of Rayleigh-waves is determined for a profile crossing the area of GDR from south to north. Two different methods of seismogram processing are used which are discussed in detail. The phase velocities found are checked for compatibility. An inversion of a common phase velocity curve is done by using hedgehog procedure.
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  • 98
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    Pure and applied geophysics 116 (1978), S. 1262-1273 
    ISSN: 1420-9136
    Keywords: Seismology ; African Rift ; P-wave velocity beneath Africa ; Upper mantle structure for Africa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract AP-wave velocity model for the upper mantle beneath eastern and southern Africa is proposed. The top 250 km of the model is characterized by relatively low velocities similar to those deduced for the upper mantle beneath the western United States of America. At greater depths, the velocities gradually change to normal mantle values.
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  • 99
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    Pure and applied geophysics 116 (1978), S. 198-211 
    ISSN: 1420-9136
    Keywords: Seismology ; theoretical ; Cagniard's technique ; Elastic waves produced by shearing traction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The displacement produced in a semi-infinite, homogeneous, isotropic elastic medium by the application of shearing traction over a circular portion of the half-space has been evaluated in exact form by Cagniard's Technique (Cagniard, 1962;Gakenheimer andMiklowitz, 1969).
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  • 100
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    Development genes and evolution 185 (1978), S. 235-248 
    ISSN: 1432-041X
    Keywords: Liver ; Primary culture ; Ultrastructure ; Albumin synthesis ; Xenopus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Electron microscopic analysis of primary cultures derived from larvalXenopus liver has shown that these cells, although they form only two-dimensional aggregates, retain and presumably also develop structural characteristics typical of liver parenchyma cells, such as bile canaliculi with microvilli and epithelial junctional complexes. As judged from structural criteria, primary cultures contain 80–90% hepatocytes. In contrast to the intact tissue, primary cultures showed excessive development of microfilaments, however. Incorporation of labeled amino acids has revealed further that the capacity for protein synthesis is maintained in culture and that synthesis of liverspecific protein albumin is maintained in vitro, even in liver cultures derived from thyrostatic tadpoles. This latter result suggests that initiation of albumin synthesis in the larval liver is probably not dependent upon thyroid hormones but rather reflects the protodifferentiated state of this tissue.
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