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  • 1
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2009. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 39 (2009): 1541-1550, doi:10.1175/2008JPO3999.1.
    Description: The response of a zonal channel to a uniform, switched-on but subsequently steady poleward outflow is presented. An eastward coastal current with a Kelvin wave’s cross-shore structure is found to be generated instantly upon initiation of the outflow. The current is essentially in geostrophic balance everywhere except for the vicinity of the outflow channel mouth, where the streamlines must cross planetary vorticity contours to feed the current. The adjustment of this region generates a plume that propagates westward at Rossby wave speeds. The cross-shore structure of the plume varies with longitude, and at any given longitude it evolves with time. The authors show that the plume evolution can be understood both conceptually and quantitatively as the westward propagation of the Kelvin current’s meridional spectrum, with each spectral element propagating at its own Rossby wave group velocity.
    Description: This work was completed at Woods Hole Oceanographic Institution while T.S. Durland was supported by the Ocean and Climate Change Institute. M.A. Spall was supported by NSF Grant OCE-0423975, and J. Pedlosky by NSF Grant OCE-0451086. T.S. Durland acknowledges additional report preparation support from NASA Grant NNG05GN98G.
    Keywords: Coastal flows ; Estuaries ; Currents ; Vorticity ; Plumes
    Repository Name: Woods Hole Open Access Server
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  • 2
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    American Meteorological Society
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2007. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 37 (2007): 148–161, doi:10.1175/JPO3003.1.
    Description: As part of a program aimed at developing a long-duration, subsurface mooring, known as Ultramoor, several modern acoustic current meters were tested. The instruments with which the authors have the most experience are the Aanderaa RCM11 and the Nortek Aquadopp, which measure currents using the Doppler shift of backscattered acoustic signals, and the Falmouth Scientific ACM, which measures changes in travel time of acoustic signals between pairs of transducers. Some results from the Doppler-based Sontek Argonaut and the travel-time-based Nobska MAVS are also reported. This paper concentrates on the fidelity of the speed measurement but also presents some results related to the accuracy of the direction measurement. Two procedures were used to compare the instruments. In one, different instruments were placed close to one another on three different deep-ocean moorings. These tests showed that the RCM11 measures consistently lower speeds than either a vector averaging current meter or a vector measuring current meter, both more traditional instruments with mechanical velocity sensors. The Aquadopp in use at the time, but since updated to address accuracy problems in low scattering environments, was biased high. A second means of testing involved comparing the appropriate velocity component of each instrument with the rate of change of pressure when they were lowered from a ship. Results from this procedure revealed no depth dependence or measurable bias in the RCM11 data, but did show biases in both the Aquadopp and Argonaut Doppler-based instruments that resulted from low signal-to-noise ratios in the clear, low scattering conditions beneath the thermocline. Improvements in the design of the latest Aquadopp have reduced this bias to a level that is not significant.
    Description: This material is based upon work supported by the National Science Foundation under Grant 9810641.
    Keywords: Currents ; Acoustic measurements ; In situ sensors
    Repository Name: Woods Hole Open Access Server
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  • 3
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2011. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 41 (2011): 889–910, doi:10.1175/2010JPO4496.1.
    Description: This paper examines interaction between a barotropic point vortex and a steplike topography with a bay-shaped shelf. The interaction is governed by two mechanisms: propagation of topographic Rossby waves and advection by the forcing vortex. Topographic waves are supported by the potential vorticity (PV) jump across the topography and propagate along the step only in one direction, having higher PV on the right. Near one side boundary of the bay, which is in the wave propagation direction and has a narrow shelf, waves are blocked by the boundary, inducing strong out-of-bay transport in the form of detached crests. The wave–boundary interaction as well as out-of-bay transport is strengthened as the minimum shelf width is decreased. The two control mechanisms are related differently in anticyclone- and cyclone-induced interactions. In anticyclone-induced interactions, the PV front deformations are moved in opposite directions by the point vortex and topographic waves; a topographic cyclone forms out of the balance between the two opposing mechanisms and is advected by the forcing vortex into the deep ocean. In cyclone-induced interactions, the PV front deformations are moved in the same direction by the two mechanisms; a topographic cyclone forms out of the wave–boundary interaction but is confined to the coast. Therefore, anticyclonic vortices are more capable of driving water off the topography. The anticyclone-induced transport is enhanced for smaller vortex–step distance or smaller topography when the vortex advection is relatively strong compared to the wave propagation mechanism.
    Description: Y. Zhang acknowledges the support of theMIT-WHOI Joint Programin Physical Oceanography, NSF OCE-9901654 and OCE-0451086. J. Pedlosky acknowledges the support of NSF OCE- 9901654 and OCE-0451086.
    Keywords: Transport ; Eddies ; Barotropic flow ; Topographic effects ; Vortices ; Currents ; Potential vorticity ; Rossby waves
    Repository Name: Woods Hole Open Access Server
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  • 4
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2012. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Atmospheric and Oceanic Technology 29 (2012): 1377–1390, doi:10.1175/JTECH-D-11-00160.1.
    Description: Estimates of surface currents over the continental shelf are now regularly made using high-frequency radar (HFR) systems along much of the U.S. coastline. The recently deployed HFR system at the Martha’s Vineyard Coastal Observatory (MVCO) is a unique addition to these systems, focusing on high spatial resolution over a relatively small coastal ocean domain with high accuracy. However, initial results from the system showed sizable errors and biased estimates of M2 tidal currents, prompting an examination of new methods to improve the quality of radar-based velocity data. The analysis described here utilizes the radial metric output of CODAR Ocean Systems’ version 7 release of the SeaSonde Radial Site Software Suite to examine both the characteristics of the received signal and the output of the direction-finding algorithm to provide data quality controls on the estimated radial currents that are independent of the estimated velocity. Additionally, the effect of weighting spatial averages of radials falling within the same range and azimuthal bin is examined to account for differences in signal quality. Applied to two month-long datasets from the MVCO high-resolution system, these new methods are found to improve the rms difference comparisons with in situ current measurements by up to 2 cm s−1, as well as reduce or eliminate observed biases of tidal ellipses estimated using standard methods.
    Description: 2013-03-01
    Keywords: Coastal flows ; Currents ; Data processing ; Data quality control ; In situ atmospheric observations ; Remote sensing
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  • 5
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2012. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 42 (2012): 2234–2253, doi:10.1175/JPO-D-12-033.1.
    Description: Meridional velocity, mass, and heat transport in the equatorial oceans are difficult to estimate because of the nonapplicability of the geostrophic balance. For this purpose a steady-state model is utilized in the equatorial Indian Ocean using NCEP wind stress and temperature and salinity data from the World Ocean Atlas 2005 (WOA05) and Argo. The results show a Somali Current flowing to the south during the winter monsoon carrying −11.5 ± 1.3 Sv (1 Sv ≡ 106 m3 s−1) and −12.3 ± 0.3 Sv from WOA05 and Argo, respectively. In the summer monsoon the Somali Current reverses to the north transporting 16.8 ± 1.2 Sv and 19.8 ± 0.6 Sv in the WOA05 and Argo results. Transitional periods are considered together and in consequence, there is not a clear Somali Current present in this period. Model results fit with in situ measurements made around the region, although Argo data results are quite more realistic than WOA05 data results.
    Description: This study has been partly funded by the MOC Project (CTM 2008- 06438) and the Spanish contribution to the Argo network (AC2009 ACI2009-0998), financed by the Spanish Government and Feder.
    Description: 2013-06-01
    Keywords: Indian Ocean ; Subtropics ; Currents ; Ocean circulation ; Transport ; Wind stress
    Repository Name: Woods Hole Open Access Server
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  • 6
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2009. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 39 (2009): 1258-1271, doi:10.1175/2008JPO4028.1.
    Description: This paper presents a set of laboratory experiments focused on how a buoyant coastal current flowing over a sloping bottom interacts with a canyon and what controls the separation, if any, of the current from the upstream canyon bend. The results show that the separation of a buoyant coastal current depends on the current width W relative to the radius of curvature of the bathymetry ρc. The flow moved across the mouth of the canyon (i.e., separated) for W/ρc 〉 1, in agreement with previous results. The present study extends previous work by examining both slope-controlled and surface-trapped currents, and using a geometry specific to investigating buoyant current–canyon interaction. The authors find that, although bottom friction is important in setting the position of the buoyant front, the separation process driven by the inertia of the flow could overcome even the strongest bathymetric influence. Application of the laboratory results to the East Greenland Current (EGC), an Arctic-origin buoyant current that is observed to flow in two branches south of Denmark Strait, suggests that the path of the EGC is influenced by the large canyons cutting across the shelf, as the range of W/ρc in the ocean spans those observed in the laboratory. What causes the formation of a two-branched EGC structure downstream of the Kangerdlugssuaq Canyon (68°N, 32°W) is still unclear, but potential mechanisms are discussed.
    Description: This work was partially funded by NSF Grant OCE-0450658. DS also received support from the Academic Programs Office of the Woods Hole Oceanographic Institution, while CC had partial support from NSF OCE-0350891.
    Keywords: Coastal flows ; Buoyancy ; Currents ; Experimental design ; Topographic effects
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  • 7
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2008. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 38 (2008): 104–120, doi:10.1175/2007JPO3686.1.
    Description: Recent studies have indicated that the North Atlantic Ocean subpolar gyre circulation undergoes significant interannual-to-decadal changes in response to variability in atmospheric forcing. There are also observations, however, suggesting that the southern limb of the subpolar gyre, namely, the eastward-flowing North Atlantic Current (NAC), may be quasi-locked to particular latitudes in the central North Atlantic by fracture zones (gaps) in the Mid-Atlantic Ridge. This could constrain the current’s ability to respond to variability in forcing. In the present study, subsurface float trajectories at 100–1000 m collected during 1997–99 and satellite-derived surface geostrophic velocities from 1992 to 2006 are used to provide an improved description of the detailed pathways of the NAC over the ridge and their relationship to bathymetry. Both the float and satellite observations indicate that in 1997–99, the northern branch of the NAC was split into two branches as it crossed the ridge, one quasi-locked to the Charlie–Gibbs Fracture Zone (CGFZ; 52°–53°N) and the other to the Faraday Fracture Zone (50°–51°N). The longer satellite time series shows, however, that this pattern did not persist outside the float sampling period and that other branching modes persisted for one or more years, including an approximately 12-month time period in 2002–03 when the strongest eastward flow over the ridge was at 49°N. Schott et al. showed how northward excursions of the NAC can temporarily block the westward flow of the Iceland–Scotland Overflow Water through the CGFZ. From the 13-yr time series of surface geostrophic velocity, it is estimated that such blocking may occur on average 6% of the time, although estimates for any given 12-month period range from 0% to 35%.
    Description: This research was supported by National Science Foundation Grants OCE-9531877 to the Woods Hole Oceanographic Institution (WHOI) and OCE-9906775 to the University of Rhode Island, by the WHOI Summer Student Fellowship Program, and by the Lawrence J. Pratt and Melinda M. Hall Endowed Fund for Interdisciplinary Research at the Woods Hole Oceanographic Institution.
    Keywords: Currents ; Topographic effects ; Interannual variability ; Forcing ; Gyres
    Repository Name: Woods Hole Open Access Server
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  • 8
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2008. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 38 (2008): 1203–1221, doi:10.1175/2007JPO3768.1.
    Description: Analyses of current time series longer than 200 days from 33 sites over the Middle Atlantic Bight continental shelf reveal a consistent mean circulation pattern. The mean depth-averaged flow is equatorward, alongshelf, and increases with increasing water depth from 3 cm s−1 at the 15-m isobath to 10 cm s−1 at the 100-m isobath. The mean cross-shelf circulation exhibits a consistent cross-shelf and vertical structure. The near-surface flow is typically offshore (positive, range −3 to 6 cm s−1). The interior flow is onshore and remarkably constant (−0.2 to −1.4 cm s−1). The near-bottom flow increases linearly with increasing water depth from −1 cm s−1 (onshore) in shallow water to 4 cm s−1 (offshore) at the 250-m isobath over the slope, with the direction reversal near the 50-m isobath. A steady, two-dimensional model (no along-isobath variations in the flow) reproduces the main features of the observed circulation pattern. The depth-averaged alongshelf flow is primarily driven by an alongshelf pressure gradient (sea surface slope of 3.7 × 10−8 increasing to the north) and an opposing mean wind stress that also drives the near-surface offshore flow. The alongshelf pressure gradient accounts for both the increase in the alongshelf flow with water depth and the geostrophic balance onshore flow in the interior. The increase in the near-bottom offshore flow with water depth is due to the change in the relative magnitude of the contributions from the geostrophic onshore flow that dominates in shallow water and the offshore flow driven by the bottom stress that dominates in deeper water.
    Description: This research was funded by Ocean Sciences Division of the National Science Foundation under Grants OCE-820773, OCE-841292, and OCE-848961.
    Keywords: Ocean models ; Ocean circulation ; Continental shelf ; Currents ; In situ observations
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  • 9
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2008. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 38 (2008): 1644-1668, doi:10.1175/2007JPO3829.1.
    Description: The mean structure and time-dependent behavior of the shelfbreak jet along the southern Beaufort Sea, and its ability to transport properties into the basin interior via eddies are explored using high-resolution mooring data and an idealized numerical model. The analysis focuses on springtime, when weakly stratified winter-transformed Pacific water is being advected out of the Chukchi Sea. When winds are weak, the observed jet is bottom trapped with a low potential vorticity core and has maximum mean velocities of O(25 cm s−1) and an eastward transport of 0.42 Sv (1 Sv ≡ 106 m3 s−1). Despite the absence of winds, the current is highly time dependent, with relative vorticity and twisting vorticity often important components of the Ertel potential vorticity. An idealized primitive equation model forced by dense, weakly stratified waters flowing off a shelf produces a mean middepth boundary current similar in structure to that observed at the mooring site. The model boundary current is also highly variable, and produces numerous strong, small anticyclonic eddies that transport the shelf water into the basin interior. Analysis of the energy conversion terms in both the mooring data and the numerical model indicates that the eddies are formed via baroclinic instability of the boundary current. The structure of the eddies in the basin interior compares well with observations from drifting ice platforms. The results suggest that eddies shed from the shelfbreak jet contribute significantly to the offshore flux of heat, salt, and other properties, and are likely important for the ventilation of the halocline in the western Arctic Ocean. Interaction with an anticyclonic basin-scale circulation, meant to represent the Beaufort gyre, enhances the offshore transport of shelf water and results in a loss of mass transport from the shelfbreak jet.
    Description: This study was supported by the National Science Foundation Office of Polar Programs under Grants 0421904 and 035268 (MS), and by the Office of Naval Research Grant N00014-02-1-0317 (RP and PF). Analysis by AJP was supported by the Office of Naval Research under Grant N00014-97-1-0135 and by the National Science Foundation under Grant OPP-9815303.
    Keywords: Arctic ; Eddies ; Transport ; Currents ; Jets
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  • 10
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2007. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography. 37 (2007): 2509-2533, doi:10.1175/JPO3123.1.
    Description: Twelve years of historical hydrographic data, spanning the period 1990–2001, are analyzed to examine the along-stream evolution of the western North Atlantic Ocean shelfbreak front and current, following its path between the west coast of Greenland and the Middle Atlantic Bight. Over 700 synoptic sections are used to construct a mean three-dimensional description of the summer shelfbreak front and to quantify the along-stream evolution in properties, including frontal strength and grounding position. Results show that there are actually two fronts in the northern part of the domain—a shallow front located near the shelf break and a deeper front centered in the core of Irminger Water over the upper slope. The properties of the deeper Irminger front erode gradually to the south, and the front disappears entirely near the Grand Banks of Newfoundland. The shallow shelfbreak front is identifiable throughout the domain, and its properties exhibit large variations from north to south, with the largest changes occurring near the Tail of the Grand Banks. Despite these structural changes, and large variations in topography, the foot of the shelfbreak front remains within 20 km of the shelf break. The hydrographic sections are also used to examine the evolution of the baroclinic velocity field and its associated volume transport. The baroclinic velocity structure consists of a single velocity core that is stronger and penetrates deeper where the Irminger front is present. The baroclinic volume transport decreases by equal amounts at the southern end of the Labrador Shelf and at the Tail of the Grand Banks. Overall, the results suggest that the Grand Banks is a geographically critical location in the North Atlantic shelfbreak system.
    Description: This work was supported by the National Science Foundation under Grants OCE00- 95261 (PF) and OCE-0450658 (RP).
    Keywords: Continental shelf ; Currents ; Atlantic Ocean ; Fronts ; Transport
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  • 11
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2014. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Climate 27 (2014): 2405–2416, doi:10.1175/JCLI-D-13-00359.1.
    Description: Several recent studies utilizing global climate models predict that the Pacific Equatorial Undercurrent (EUC) will strengthen over the twenty-first century. Here, historical changes in the tropical Pacific are investigated using the Simple Ocean Data Assimilation (SODA) reanalysis toward understanding the dynamics and mechanisms that may dictate such a change. Although SODA does not assimilate velocity observations, the seasonal-to-interannual variability of the EUC estimated by SODA corresponds well with moored observations over a ~20-yr common period. Long-term trends in SODA indicate that the EUC core velocity has increased by 16% century−1 and as much as 47% century−1 at fixed locations since the mid-1800s. Diagnosis of the zonal momentum budget in the equatorial Pacific reveals two distinct seasonal mechanisms that explain the EUC strengthening. The first is characterized by strengthening of the western Pacific trade winds and hence oceanic zonal pressure gradient during boreal spring. The second entails weakening of eastern Pacific trade winds during boreal summer, which weakens the surface current and reduces EUC deceleration through vertical friction. EUC strengthening has important ecological implications as upwelling affects the thermal and biogeochemical environment. Furthermore, given the potential large-scale influence of EUC strength and depth on the heat budget in the eastern Pacific, the seasonal strengthening of the EUC may help reconcile paradoxical observations of Walker circulation slowdown and zonal SST gradient strengthening. Such a process would represent a new dynamical “thermostat” on CO2-forced warming of the tropical Pacific Ocean, emphasizing the importance of ocean dynamics and seasonality in understanding climate change projections.
    Description: EJDis supported by NSFGrantsOCE-1031971 and OCE-1233282. KBK is supported by NSF Grant OCE-1233282.
    Description: 2014-09-15
    Keywords: Tropics ; Currents ; Ocean dynamics ; Atmosphere-ocean interaction ; Climate variability ; Reanalysis data
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  • 12
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2014. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Atmospheric and Oceanic Technology 21 (2014): 2015–2025, doi:10.1175/JTECH-D-13-00262.1.
    Description: The NOAA Tropical Atmosphere Ocean (TAO) moored array has, for three decades, been a valuable resource for monitoring and forecasting El Niño–Southern Oscillation and understanding physical oceanographic as well as coupled processes in the tropical Pacific influencing global climate. Acoustic Doppler current profiler (ADCP) measurements by TAO moorings provide benchmarks for evaluating numerical simulations of subsurface circulation including the Equatorial Undercurrent (EUC). Meanwhile, the Sea Education Association (SEA) has been collecting data during repeat cruises to the central equatorial Pacific Ocean (160°–126°W) throughout the past decade that provide useful cross validation and quantitative insight into the potential for stationary observing platforms such as TAO to incur sampling biases related to the strength of the EUC. This paper describes some essential sampling characteristics of the SEA dataset, compares SEA and TAO velocity measurements in the vicinity of the EUC, shares new insight into EUC characteristics and behavior only observable in repeat cross-equatorial sections, and estimates the sampling bias incurred by equatorial TAO moorings in their estimates of the velocity and transport of the EUC. The SEA high-resolution ADCP dataset compares well with concurrent TAO measurements (RMSE = 0.05 m s−1; R2 = 0.98), suggests that the EUC core meanders sinusoidally about the equator between ±0.4° latitude, and reveals a mean sampling bias of equatorial measurements (e.g., TAO) of the EUC’s zonal velocity of −0.14 ± 0.03 m s−1 as well as a ~10% underestimation of EUC volume transport. A bias-corrected monthly record and climatology of EUC strength at 140°W for 1990–2010 is presented.
    Description: The authors thank the NSF Physical Oceanography program (OCE-1233282) and the WHOI Academic Programs Office for funding.
    Description: 2015-03-01
    Keywords: Pacific Ocean ; Tropics ; Currents ; Ocean dynamics ; Buoy observations ; Sampling
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  • 13
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2010. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 40 (2010): 2768–2777, doi:10.1175/2010JPO4461.1.
    Description: Although sustained observations yield a description of the mean equatorial current system from the western Pacific to the eastern terminus of the Tropical Atmosphere Ocean (TAO) array, a comprehensive observational dataset suitable for describing the structure and pathways of the Equatorial Undercurrent (EUC) east of 95°W does not exist and therefore climate models are unconstrained in a region that plays a critical role in ocean–atmosphere coupling. Furthermore, ocean models suggest that the interaction between the EUC and the Galápagos Islands (92°W) has a striking effect on the basic state and coupled variability of the tropical Pacific. To this end, the authors interpret historical measurements beginning with those made in conjunction with the discovery of the Pacific EUC in the 1950s, analyze velocity measurements from an equatorial TAO mooring at 85°W, and analyze a new dataset from archived shipboard ADCP measurements. Together, the observations yield a possible composite description of the EUC structure and pathways in the eastern equatorial Pacific that may be useful for model validation and guiding future observation.
    Description: Karnauskas acknowledges the WHOI Penzance Endowed Fund in Support of Assistant Scientists.
    Keywords: Atmosphere-ocean interaction ; Currents ; In situ observations ; Model evaluation/performance ; Pacific Ocean ; Tropics
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  • 14
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2009. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 39 (2009): 3162-3175, doi:10.1175/2009JPO4239.1.
    Description: This study analyzes anisotropic properties of the material transport by eddies and eddy-driven zonal jets in a general circulation model of the North Atlantic through the analysis of Lagrangian particle trajectories. Spreading rates—defined here as half the rate of change in the particle dispersion—in the zonal direction systematically exceed the meridional rates by an order of magnitude. Area-averaged values for the upper-ocean zonal and meridional spreading rates are approximately 8100 and 1400 m2 s−1, respectively, and in the deep ocean they are 2400 and 200 m2 s−1. The results demonstrate that this anisotropy is mainly due to the action of the transient eddies and not to the shear dispersion associated with the time-mean jets. This property is consistent with the fact that eddies in this study have zonally elongated shapes. With the exception of the upper-ocean subpolar gyre, eddies also cause the superdiffusive zonal spreading, significant variations in the spreading rate in the vertical and meridional directions, and the difference between the westward and eastward spreading.
    Description: Funding for IK was provided by NSF Grants OCE 0346178, 0749722, and 0842834. Funding for PB was provided by NSF Grants OCE 0344094 and OCE 0725796 and by the research grant from the Newton Trust of the University of Cambridge. For JP the acknowledgement is to NSF OCE-0451086.
    Keywords: Eddies ; Transport ; Currents ; North Atlantic Ocean
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  • 15
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2007. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 37 (2007): 2563-2569, doi:10.1175/JPO3134.1.
    Description: Along the Taiwan Strait (〈100 m in depth) a northeastward flow persists in all seasons despite the annually averaged wind stress that is strongly southwestward. The forcing mechanism of this countercurrent is examined by using a simple ocean model. The results from a suite of experiments demonstrate that it is the Kuroshio that plays the deciding role for setting the flow direction along the Taiwan Strait. The momentum balance along the strait is mainly between the wind stress, friction, and pressure gradient. Since both wind stress and friction act against the northward flow, it is most likely the pressure gradient that forces the northward flow, as noted in some previous studies. What remains unknown is why there is a considerable pressure difference between the southern and northern strait. The Kuroshio flows along the east coast of Taiwan, and thus the western boundary current layer dynamics applies there. Integrating the momentum equation along Taiwan’s east coast shows that there must be a pressure difference between the southern and the northern tip of Taiwan to counter a considerable friction exerted by the mighty Kuroshio. This same pressure difference is also felt on the other side of the island where it forces the northward flow through Taiwan Strait. The model shows that the local wind stress acts to dampen this northward flow. This mechanism can be illustrated by an integral constraint for flow around an island.
    Description: This study has been supported by National Science Foundation through Grant OCE-0351055.
    Keywords: Ocean circulation ; Wind ; Currents
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  • 16
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2010. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 40 (2010): 2679–2695, doi:10.1175/2010JPO4395.1.
    Description: Observations of stratification and currents between June 2007 and March 2009 reveal a strong overflow between 400- and 570-m depth from the Panay Strait into the Sulu Sea. The overflow water is derived from approximately 400 m deep in the South China Sea. Temporal mean velocity is greater than 0.75 m s−1 at 50 m above the 570-m Panay Sill. Empirical orthogonal function analysis of a mooring time series shows that the flow is dominated by the bottom overflow current with little seasonal variance. The overflow does not descend below 1250 m in the Sulu Sea but rather settles above high-salinity deep water derived from the Sulawesi Sea. The mean observed overflow transport at the sill is 0.32 × 106 m3 s−1. The observed transport was used to calculate a bulk diapycnal diffusivity of 4.4 × 10−4 m2 s−1 within the Sulu Sea slab (575–1250 m) ventilated from Panay Strait. Analysis of Froude number variation across the sill shows that the flow is hydraulically controlled. A suitable hydraulic control model shows overflow transport equivalent to the observed overflow. Thorpe-scale estimates show turbulent dissipation rates up to 5 × 10−7 W kg−1 just downstream of the supercritical to subcritical flow transition, suggesting a hydraulic jump downstream of the sill.
    Description: This work was supported by the Office of Naval Research Grant N00014-09-1-0582 to Lamont-Doherty Earth Observatory of Columbia University; Grants ONR-13759000 and N00014-09-1-0582 to the Woods Hole Oceanographic Institution; Grant ONR-N00014-06-1-0690 to Scripps Institute of Oceanography; and a National Defense Science and Engineering Graduate Fellowship.
    Keywords: Transport ; Dynamics ; Topographic effects ; Currents ; Empirical orthogonal functions
    Repository Name: Woods Hole Open Access Server
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  • 17
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2011. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 41 (2011): 911–925, doi:10.1175/2011JPO4498.1.
    Description: Motivated by discrepancies between Eulerian transport estimates and the behavior of Lagrangian surface drifters, near-surface transport pathways and processes in the North Atlantic are studied using a combination of data, altimetric surface heights, statistical analysis of trajectories, and dynamical systems techniques. Particular attention is paid to the issue of the subtropical-to-subpolar intergyre fluid exchange. The velocity field used in this study is composed of a steady drifter-derived background flow, upon which a time-dependent altimeter-based perturbation is superimposed. This analysis suggests that most of the fluid entering the subpolar gyre from the subtropical gyre within two years comes from a narrow region lying inshore of the Gulf Stream core, whereas fluid on the offshore side of the Gulf Stream is largely prevented from doing so by the Gulf Stream core, which acts as a strong transport barrier, in agreement with past studies. The transport barrier near the Gulf Stream core is robust and persistent from 1992 until 2008. The qualitative behavior is found to be largely independent of the Ekman drift.
    Description: This work was supported by the National Science Foundation Grants CMG-82469600 and CMG-82579600 and by the Office of Naval Research Grant ONR-13108700.
    Keywords: Atlantic Ocean ; Transport ; Gyres ; Lagrangian circulation/transport ; Tracers ; Currents ; Meridional overturning circulation
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  • 18
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2011. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Climate 24 (2011): 762-777, doi:10.1175/2010JCLI3731.1.
    Description: The meridional shifts of the Oyashio Extension (OE) and of the Kuroshio Extension (KE), as derived from high-resolution monthly sea surface temperature (SST) anomalies in 1982–2008 and historical temperature profiles in 1979–2007, respectively, are shown based on lagged regression analysis to significantly influence the large-scale atmospheric circulation. The signals are independent from the ENSO teleconnections, which were removed by seasonally varying, asymmetric regression onto the first three principal components of the tropical Pacific SST anomalies. The response to the meridional shifts of the OE front is equivalent barotropic and broadly resembles the North Pacific Oscillation/western Pacific pattern in a positive phase for a northward frontal displacement. The response may reach 35 m at 250 hPa for a typical OE shift, a strong sensitivity since the associated SST anomaly is 0.5 K. However, the amplitude, but not the pattern or statistical significance, strongly depends on the lag and an assumed 2-month atmospheric response time. The response is stronger during fall and winter and when the front is displaced southward. The response to the northward KE shifts primarily consists of a high centered in the northwestern North Pacific and hemispheric teleconnections. The response is also equivalent barotropic, except near Kamchatka, where it tilts slightly westward with height. The typical amplitude is half as large as that associated with OE shifts.
    Description: This work was supported in part by the L’Institut universitaire de France (CF), the WHOI Heyman fellowship, and the NASAGrant withAwardNNX09AF35G(Y.-O. K), and grants through NOAA’s Climate Variability and Predictability Program (MAA).
    Keywords: Atmospheric circulation ; Currents
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  • 19
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2013. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Atmospheric and Oceanic Technology 30 (2013): 2465–2477, doi:10.1175/JTECH-D-13-00032.1.
    Description: Seven current meters representing four models on a stiffly buoyed mooring were placed for an 11-month deployment to intercompare their velocity measurements: two vector-measuring current meters (VMCMs), two Aanderaa recording current meter (RCM) 11s, two Aanderaa SEAGUARDs, and a Nortek Aquadopp. The current meters were placed 6-m apart from each other at about 4000-m depth in an area of Drake Passage expected to have strong currents, nearly independent of depth near the bottom. Two high-current events occurred in bursts of semidiurnal pulses lasting several days, one with peak speeds up to 67 cm s−1 and the other above 35 cm s−1. The current-speed measurements all agreed within 7% of the median value when vector averaged over simultaneous time intervals. The VMCMs, chosen as the reference measurements, were found to measure the median of the mean-current magnitudes. The RCM11 and SEAGUARD current speeds agreed within 2% of the median at higher speeds (35–67 cm s−1), whereas in lower speed ranges (0–35 cm s−1) the vector-averaged speeds for the RCM11 and SEAGUARD were 4%–5% lower and 3%–5% higher than the median, respectively. The shorter-record Aquadopp current speeds were about 6% higher than the VMCMs over the range (0–40 cm s−1) encountered.
    Description: This work was supported by U.S. National Science Foundation Grants ANT-0635437 and ANT-0636493.
    Description: 2014-04-01
    Keywords: Currents ; Acoustic measurements/effects ; In situ oceanic observations ; Instrumentation/sensors
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  • 20
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2014. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Atmospheric and Oceanic Technology 31 (2014): 945–966, doi:10.1175/JTECH-D-13-00146.1.
    Description: This study investigated the correspondence between the near-surface drifters from a mass drifter deployment near Martha’s Vineyard, Massachusetts, and the surface current observations from a network of three high-resolution, high-frequency radars to understand the effects of the radar temporal and spatial resolution on the resulting Eulerian current velocities and Lagrangian trajectories and their predictability. The radar-based surface currents were found to be unbiased in direction but biased in magnitude with respect to drifter velocities. The radar systematically underestimated velocities by approximately 2 cm s−1 due to the smoothing effects of spatial and temporal averaging. The radar accuracy, quantified by the domain-averaged rms difference between instantaneous radar and drifter velocities, was found to be about 3.8 cm s−1. A Lagrangian comparison between the real and simulated drifters resulted in the separation distances of roughly 1 km over the course of 10 h, or an equivalent separation speed of approximately 2.8 cm s−1. The effects of the temporal and spatial radar resolution were examined by degrading the radar fields to coarser resolutions, revealing the existence of critical scales (1.5–2 km and 3 h) beyond which the ability of the radar to reproduce drifter trajectories decreased more rapidly. Finally, the importance of the different flow components present during the experiment—mean, tidal, locally wind-driven currents, and the residual velocities—was analyzed, finding that, during the study period, a combination of tidal, locally wind-driven, and mean currents were insufficient to reliably reproduce, with minimal degradation, the trajectories of real drifters. Instead, a minimum combination of the tidal and residual currents was required.
    Description: I.R. was supported by the WHOI Coastal Ocean Institute Project 27040148 and by the WHOI Access to the Sea Program 27500036. I.R. and A.K. acknowledge support fromthe NSF project 83264600. A.K. acknowledges support from the Massachusetts Clean Energy Center (MassCEC) via the New England Marine Renewable Energy Center (MREC).
    Description: 2014-10-01
    Keywords: Coastal flows ; Currents ; Lagrangian circulation/transport ; Trajectories ; Radars/Radar observations
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  • 21
    Publication Date: 2022-05-26
    Description: Author Posting. © American Geophysical Union, 2014. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 119 (2014): 2987–3001, doi:10.1002/2014JC009839.
    Description: Observations of waves, flows, and water levels collected for a month in and near a long, narrow, shallow (∼ 3000 m long, 1000 m wide, and 5 m deep), well-mixed ocean inlet are used to evaluate the subtidal (periods 〉 30 h) along-inlet momentum balance. Maximum tidal flows in the inlet were about 1.5 m/s and offshore significant wave heights ranged from about 0.5 to 2.5 m. The dominant terms in the local (across the km-wide ebb shoal) along-inlet momentum balance are the along-inlet pressure gradient, the bottom stress, and the wave radiation-stress gradient. Estimated nonlinear advective acceleration terms roughly balance in the channel. Onshore radiation-stress gradients owing to breaking waves enhance the flood flows into the inlet, especially during storms.
    Description: Funding was provided by the Office of Naval Research, the Office of the Assistant Secretary of Defense for Research and Engineering, and a National Defense Science and Engineering Graduate Fellowship.
    Description: 2014-11-20
    Keywords: Inlet ; Wind waves ; Currents ; Radiation stress
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  • 22
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2010. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Climate 23 (2010): 3249-3281, doi:10.1175/2010JCLI3343.1.
    Description: Ocean–atmosphere interaction over the Northern Hemisphere western boundary current (WBC) regions (i.e., the Gulf Stream, Kuroshio, Oyashio, and their extensions) is reviewed with an emphasis on their role in basin-scale climate variability. SST anomalies exhibit considerable variance on interannual to decadal time scales in these regions. Low-frequency SST variability is primarily driven by basin-scale wind stress curl variability via the oceanic Rossby wave adjustment of the gyre-scale circulation that modulates the latitude and strength of the WBC-related oceanic fronts. Rectification of the variability by mesoscale eddies, reemergence of the anomalies from the preceding winter, and tropical remote forcing also play important roles in driving and maintaining the low-frequency variability in these regions. In the Gulf Stream region, interaction with the deep western boundary current also likely influences the low-frequency variability. Surface heat fluxes damp the low-frequency SST anomalies over the WBC regions; thus, heat fluxes originate with heat anomalies in the ocean and have the potential to drive the overlying atmospheric circulation. While recent observational studies demonstrate a local atmospheric boundary layer response to WBC changes, the latter’s influence on the large-scale atmospheric circulation is still unclear. Nevertheless, heat and moisture fluxes from the WBCs into the atmosphere influence the mean state of the atmospheric circulation, including anchoring the latitude of the storm tracks to the WBCs. Furthermore, many climate models suggest that the large-scale atmospheric response to SST anomalies driven by ocean dynamics in WBC regions can be important in generating decadal climate variability. As a step toward bridging climate model results and observations, the degree of realism of the WBC in current climate model simulations is assessed. Finally, outstanding issues concerning ocean–atmosphere interaction in WBC regions and its impact on climate variability are discussed.
    Description: Funding for LT was provided by the NASA-sponsored Ocean Surface Topography Science Team, under Contract 1267196 with the University of Washington, administered by the Jet Propulsion Laboratory. HN was supported in part by the Grant-in-Aid 18204044 by the Japan Society for Promotion for Science (JSPS) and the Global Environment Research Fund (S-5) of the Japanese Ministry of Environment. YK was supported by the Kerr Endowed Fund and Penzance Endowed Fund.
    Keywords: Currents ; Sea surface temperature ; Anomalies ; Large-scale motions ; Oceanic mixed layer ; Northern Hemisphere
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  • 23
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2011. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 41 (2011): 241-246, doi:10.1175/2010JPO4557.1.
    Description: The vertical dispersion of a tracer released on a density surface near 1500-m depth in the Antarctic Circumpolar Current west of Drake Passage indicates that the diapycnal diffusivity, averaged over 1 yr and over tens of thousands of square kilometers, is (1.3 ± 0.2) × 10−5 m2 s−1. Diapycnal diffusivity estimated from turbulent kinetic energy dissipation measurements about the area occupied by the tracer in austral summer 2010 was somewhat less, but still within a factor of 2, at (0.75 ± 0.07) × 10−5 m2 s−1. Turbulent diapycnal mixing of this intensity is characteristic of the midlatitude ocean interior, where the energy for mixing is believed to derive from internal wave breaking. Indeed, despite the frequent and intense atmospheric forcing experienced by the Southern Ocean, the amplitude of finescale velocity shear sampled about the tracer was similar to background amplitudes in the midlatitude ocean, with levels elevated to only 20%–50% above the Garrett–Munk reference spectrum. These results add to a long line of evidence that diapycnal mixing in the interior middepth ocean is weak and is likely too small to dictate the middepth meridional overturning circulation of the ocean.
    Description: This material is based upon work supported by the National Science Foundation Grants OCE-0622825,OCE-0622670, OCE-0622630, and OCE-0623177.
    Keywords: Diapycnal mixing ; Currents ; Antarctica ; Ocean circulation ; Meridional overturning circulation
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  • 24
    Publication Date: 2022-05-26
    Description: © The Authors, 2010. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Coral Reefs 30 (2011): 23-38, doi:10.1007/s00338-010-0705-3.
    Description: Sediment traps are commonly used as standard tools for monitoring “sedimentation” in coral reef environments. In much of the literature where sediment traps were used to measure the effects of “sedimentation” on corals, it is clear from deployment descriptions and interpretations of the resulting data that information derived from sediment traps has frequently been misinterpreted or misapplied. Despite their widespread use in this setting, sediment traps do not provide quantitative information about “sedimentation” on coral surfaces. Traps can provide useful information about the relative magnitude of sediment dynamics if trap deployment standards are used. This conclusion is based first on a brief review of the state of knowledge of sediment trap dynamics, which has primarily focused on traps deployed high above the seabed in relatively deep water, followed by our understanding of near-bed sediment dynamics in shallow-water environments that characterize coral reefs. This overview is followed by the first synthesis of near-bed sediment trap data collected with concurrent hydrodynamic information in coral reef environments. This collective information is utilized to develop nine protocols for using sediment traps in coral reef environments, which focus on trap parameters that researchers can control such as trap height (H), trap mouth diameter (D), the height of the trap mouth above the substrate (z o ), and the spacing between traps. The hydrodynamic behavior of sediment traps and the limitations of data derived from these traps should be forefront when interpreting sediment trap data to infer sediment transport processes in coral reef environments.
    Keywords: Sediment trap ; Coral reefs ; Sedimentation ; Waves ; Currents ; Shear stress
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  • 25
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    European journal of nutrition 36 (1997), S. 299-302 
    ISSN: 1436-6215
    Keywords: Energy retention ; protein retention ; fat retention ; growth ; body composition ; broilers ; Energieansatz ; Proteinansatz ; Fettansatz ; Wachstum ; Körperzusammensetzung ; Broiler
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Mit steigender Aufnahme erhöht sich der Körperfettgehalt und der Körperproteingehalt nimmt ab. Es wird oft angenommen, daß jede Zunahme im Ansatz mit mehr Fett und weniger Protein verbunden ist. Experimentelle Ergebnisse widerlegen jedoch diese Annahme. In zwei Experimenten mit männlichen Broilerhühnern wurden die Tiere auf einem Niveau von 60 % und 100 % der empfohlenen Energieaufnahme gefüttert. Die Körperzusammensetzung bei 1500 g zeigte, wie erwartet, daß sich bei steigenden Aufnahmen der Körperfettgchalt vergrößert und der Körperproteingehalt abnimmt. Fettund Proteinansatz waren linear mit der totalen Energieretention (ER) korreliert. Das bedeutet, daß jede Zunahme im Ansatz den gleichen Protein- und Fettgehalt besitzt. Wenn der Fettansatz gleich Null ist wird nur Protein, etwa 50 % des maximalen Ansatzes, retiniert. Wenn ER=O ist, wird Protein angesetzt und Fett mobilisiert. Energie-und N-Bilanzuntersuchungen bestätigen die konstante Zusammensetzung jeder Vergrößerung des Ansatzes. Die Ergebnisse beider Experimente zeigen, daß die ER aus zwei Komponenten besteht: einem basalen konstanten täglichen Proteinansatz und einer variablen zusätzlichen ER, die hauptsächlich aus Fett besteht. Der basale Proteinansatz beträgt etwa 50 % des maximalen Ansatzes. Mit steigenden Energieaufnahmen wird der basale Proteinansatz mit einer zusätzlichen Menge von Protein und Fett im konstanten Verhältnis ergänzt.
    Notes: Summary With increasing intakes the body fat content increases and that of protein decreases. It is most often assumed that this is brought about because each increment in retention contains more fat and less protein. Experimental results, however, showed that this explanation is not true. In two experiments male broiler chickens were fed at levels between 60 and 100 % of recommended energy intake. Body composition at 1500 g showed, as expected, that with increasing intakes body fat content increased and protein content decreased. Both fat and protein retention per day were linearly related to total energy retention (ER). This means that each increment in retention has the same protein and fat content. At zero fat retention only protein was retained, about 50 % of maximal retention. At zero ER protein was retained and fat mobilized. Energy and N balance experiments confirmed the constant composition of each increment in retention. The results of both experiments show that total ER consisted of two components: a basic constant daily protein retention and a variable additional ER, mainly consisting of fat. The basic protein retention is about half of maximal retention. With increasing energy intakes the basic protein retention is combined with an additional amount of protein and fat in a constant ratio.
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  • 26
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    European journal of nutrition 36 (1997), S. 332-335 
    ISSN: 1436-6215
    Keywords: Nutrient intake ; protein metabolism ; protein synthesis ; growth ; energy expenditures ; Nährstoffaufnahme ; Proteinstoffwechsel ; Proteinsynthese ; Wachstum ; Energieaufwand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Eine Zunahme von fettfreiem Gewebe tritt auf, wenn die Proteinsynthese größer ist als der Proteinabbau. Obwohl während des Wachstums von der Geburt bis zur Reife die absoluten Proteinsynthese- und -abbauraten ansteigen, nehmen dagegen die fraktionellen Raten ab. Bcide Prozesse reagieren auf die Nährstoffaufnahme. Es gibt aber deutliche Unterschiede zwischen den verschiedenen Geweben. Protein, Kohlenhydrate und Fett können den Proteinansatz bei unreifen Tieren und Kindern stimulieren. Die zugrundeliegenden Mechanismen und die Energieaufwendungen scheinen jedoch unterschiedlich zu sein.
    Notes: Summary Lean tissue growth occurs when the rate of protein synthesis exceeds the rate of protein breakdown. Althoughabsolute rates of protein synthesis and breakdown rise during growth from birth to maturityfractional rates fall. Both these processes are sensitive to nutrient intake but responses to feeding vary greatly amongst different tissues. Protein, carbohydrate and fat can all stimulate body protein accretion in immature animals and in children but the mechanisms by which they do so, and the energy expenditures involved, seem to be different.
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  • 27
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    Empirical economics 24 (1999), S. 23-44 
    ISSN: 1435-8921
    Keywords: Key words: Cointegration ; convergence ; growth ; Kalman filter ; JEL classifications: C22 ; O47 ; O57
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract. Augmented Dickey Fuller (ADF) and Kalman filter convergence tests are applied to annual GDPs per head to 16 industrialised countries from 1890 to 1989. Results favour convergence towards the US with a structural break following the Second World War. Estimates suggest that steady-states were higher after the war and that speeds of convergence are different across countries. The Kalman filter method dismissed the no convergence hypothesis more often than its ADF counterpart. This could explain the apparent contradiction in earlier empirical work on similar data sets (cross-section methods tended to favour convergence while time series methods were unable to dismiss the no convergence hypothesis.)
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  • 28
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    Entomologia experimentalis et applicata 80 (1996), S. 141-144 
    ISSN: 1570-7458
    Keywords: Polygonia c-album ; Nymphalidae ; host-plant selection ; growth ; size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 29
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    Entomologia experimentalis et applicata 80 (1996), S. 163-165 
    ISSN: 1570-7458
    Keywords: olfaction ; EAG ; sensory physiology ; antennal sensitivity ; interspecies hybrids ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 30
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    Entomologia experimentalis et applicata 91 (1999), S. 359-368 
    ISSN: 1570-7458
    Keywords: Barbarea vulgaris ssp. arcuata ; Cruciferae ; Phyllotreta nemorum ; Chrysomelidae ; Alticinae ; flea beetle ; plant defence ; host plant range ; near-isogenic ; Y-linkage ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Y-linked gene (R-gene) in the flea beetle Phyllotreta nemorum L. (Coleoptera: Chrysomelidae: Alticinae) confer the ability of larvae to survive on types of the plant Barbarea vulgaris R.Br. (Brassicaceae) which are immune to attack by susceptible conspecifics. Two near-isogenic flea beetle lines were developed. The YE-line contained the Y-linked R-gene, and male larvae from this line survived on B. vulgaris. The ST-line did not contain the gene and did not survive on the plant. The YE-line had been developed through 8–9 generations of backcrosses (YE-males with ST-females) and the two lines were considered to be isogenic except for genes located on the Y-chromosome. A single copy of the Y-linked gene is sufficient to transfer a susceptible genotype (ST) into a resistant genotype (YE) which is able to utilize a plant that is immune to attack by specimens without R-genes. The Y-linked gene had no effects on survival on other plant species tested. The gene did not have any effect on developmental times and weights of adult beetles reared on other plants than B. vulgaris. Developmental times of larvae with the Y-linked gene were longer on B. vulgaris than on normal host plants, R. sativus and S. arvensis, but the adults obtained the same size on these plant species. No trade-offs of the Y-linked gene were discovered. The results suggest that the occurrence of the Y-linked gene is a derived trait which has enabled the flea beetle to expand its host plant range. The evolution of a host shift to B. vulgaris seems not to be favoured by the presence of this single gene.
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  • 31
    ISSN: 1570-7458
    Keywords: amylase inhibitor ; red kidney bean ; hard red winter wheat ; growth ; insects ; beetles ; plant resistance ; stored products ; protease inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Insect α-amylase inhibiting and/or growth inhibiting activities of proteinaceous inhibitors from red kidney bean (Phaseolus vulgaris) and hard red winter wheat (Triticum aestivum) were examined. The bean inhibitor was most effectivein vitro against α-amylases from the red flour beetle (Tribolium castaneum) and the confused flour beetle (T. confusum), followed by those from the rice weevil (Sitophilus oryzae) and yellow mealworm (Tenebrio molitor). The insect enzymes were from two- to 50-fold more susceptible than human salivary α-amylase. When the inhibitors were added at a 1% level to a wheat flour plus germ diet, the growth of red flour beetle larvae was slowed relative to that of the control group of larvae, with the bean inhibitor being more effective than the wheat inhibitor. Development of both the red flour beetle and flat grain beetle (Cryptolestes pusillus) was delayed by 1% bean inhibitor, but development of the sawtoothed grain beetle (Oryzaephilus surinamensis) and lesser grain borer (Rhyzopertha dominica) was not affected by either the bean or wheat inhibitor at the 1% level. Rice weevil adults fed a diet containing 1% bean or wheat inhibitor exhibited more mortality than weevils fed the control diet. When the wheat amylase inhibitor was combined with a cysteine protease inhibitor, E-64, and fed to red flour beetle larvae, a reduction in the growth rate and an increase in the time required for adult eclosion occurred relative to larvae fed either of the inhibitors separately. The bean inhibitor was just as effective alone as when it was combined with the protease inhibitor. These results demonstrate that plant inhibitors of insect digestive enzymes act as growth inhibitors of insects and possibly as plant defense proteins, and open the way to the use of the genes of these inhibitors for genetically improving the resistance of cereals to storage pests.
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  • 32
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    Acta biotheoretica 46 (1998), S. 141-156 
    ISSN: 1572-8358
    Keywords: locomotion ; Squamata ; lizards ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In lower quadrupedal vertebrates locomotor efficiency seems to result from the associate movements of the axial and appendicular systems, which are totally independent in structure and embryological origin. The curvature of the trunk, produced by a standing wave, magnifies the propulsive action of the limbs. In intermediate forms, the association of an elongate trunk with limbs reduced in size brings about functional consequences which may be noticeably diverse according to the degree of trunk elongation and limb reduction. According to environmental constraints, animals search for better locomotor efficiency, which implies the maintenance or breakage of this association of both locomotor systems. In some cases, limb action on the ground is added to the axial wave action through a perfect mutual adjustment of rhythmic activity, until mechanical inefficiency of the limbs is reached by possible loss of contact with the ground. In other cases, the limbs dragged on the ground during the stance phase act against the axial action or, on the contrary, are inhibited by the axial system. A review of available data tries to contribute to an understanding of the respective roles of both systems in the transition to limblessness.
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  • 33
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    Entomologia experimentalis et applicata 79 (1996), S. 335-344 
    ISSN: 1570-7458
    Keywords: alkaloid ; developmental time ; early instars ; growth ; Manduca sexta ; molting duration ; phenolics ; plant-herbivore interactions ; thermal regime
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To understand the mechanisms underlying plant-insect herbivore interactions, it is necessary to examine the simultaneous effects of temperature, food quality and larval age. We examined the simultaneous effects of three allelochemicals (tomatine, rutin and chlorogenic acid) on the performance of first and second instar Manduca sexta larvae under two representative thermal regimes 21 : 10°C and 26 : 15°C for spring and summer, respectively. Thermal regime and allelochemicals interacted to influence the time from egg hatch to ecdysis to the third instar. On average, it took about half as much time to reach the third instar at 26 : 15°C as it did at 21 : 10°C. Separately, tomatine and rutin had a negative effect on developmental time from egg hatch to the third instar, but their simutaneous effects were not additive. Chlorogenic acid significantly reduced the negative effect of tomatine. The magnitude of the allelochemical effect was larger at the cooler thermal regime compared to the warmer regime. For instance, chlorogenic acid by itself had no effect at the 26 : 15°C regime, but at the 21 : 10°C regime it significantly shortened total developmental time. The effect of chlorogenic acid on stadium duration was distinctly different for the two instars. Chlorogenic acid shortened stadium duration of first instar larvae. However, depending on thermal regime and the presence of tomatine, chlorogenic acid had a negative, positive or neutral effect on stadium duration of second instar larvae. Molting duration of second instar larvae was shortened by a half day at the warmer thermal regime but was not affected by the allelochemicals. Final larval weight was influenced by rutin and chlorogenic acid. Caterpillars fed diets containing 20 μmoles of rutin were on average 10% lighter than those fed plain diet, whereas those fed diets containing 20 μmoles of chlorogenic adic were on average 7% heavier. However, the effect of chlorogenic acid depended on thermal regime. Overall, our results indicated that: 1) temperature and food quality can interact to influence insect performance and 2) these effects are influenced by larval age.
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    Entomologia experimentalis et applicata 80 (1996), S. 7-13 
    ISSN: 1570-7458
    Keywords: host-plant selection ; sensory physiology ; neural coding ; deterrents ; peripheral interactions ; receptor sites ; genetics of insects ; evolution
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    Notes: Abstract Recent advances in our understanding of the relationship between chemosensory and behavioural responses to phytochemicals come from a number of studies on ovipositional and food selection behaviour of flies, butterflies, moths and beetles. Establishing input-output relationships has provided insight into the way in which the activity of chemoreceptors is translated into host-plant selection behaviour. This was achieved for both the qualitative contrast acceptance/rejection and for quantifiable preference hierarchies. By now it is clear that the subtlety of coding the complex phytochemical profiles offered by potential host plants relies on across-fibre patterns or ensemblefiring of taste neurons. Progress along these lines depends on unravelling processing pathways in the central nervous system, still a largely unexplored area in herbivorous insects. Increased interest can be noted for the mechanisms operating during the most peripheral events of chemoreception: the interaction of phytochemical and chemoreceptor, determining the specificity of recognition. Evidence for ‘peripheral integration’ has accumulated. Deterrent receptors have an especially puzzling nature. Although such cells respond to a wide array of structurally diverse secondary plant metabolites, their sensitivity profile differs between closely related species. To what extent membrane-bound receptor molecules are involved and what degree of specificity is conferred by these, is largely unknown. Sensitivity to a certain group or class of compounds is determined by single genes in several cases. This allows for a scenario in which single gene mutations affect stimulus-receptor interactions, which might concurrently affect host-plant selection behaviour.
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    Entomologia experimentalis et applicata 80 (1996), S. 320-324 
    ISSN: 1570-7458
    Keywords: multitrophic interactions ; phylogeny ; evolution ; fitness
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    Entomologia experimentalis et applicata 80 (1996), S. 443-451 
    ISSN: 1570-7458
    Keywords: beta-carboline alkaloid ; Trichoplusia ni ; harmane ; harmaline ; amitriptyline ; imipramine ; monoamine oxidase inhibitor ; tricyclics ; growth ; feeding behavior
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    Notes: Abstract In this study we investigated the effects of two naturally occurring beta-carboline alkaloids and two synthetic tricyclic antidepressants on the growth and food consumption of fifth instar larvae of the cabbage looper, Trichoplusia ni Hübner (Lepidoptera: Noctuidae). In artificial diets at high concentrations (3,000 ppm), harmane, amitriptyline, and imipramine reduce growth and feeding; harmane reduced feeding consistently at a lower concentration (200 ppm). In animals other than insects, beta-carboline alkaloids inhibit monoamine oxidase (MAO) activity and thus affect rates of disposition of serotonin and other monoamine neurotransmitters. Because brain serotonin levels are associated with variation in rates of carbohydrate and protein intake in insects, the effects of beta-carboline alkaloid ingestion on dietary self-selection behavior were examined. Choosing between diets lacking carbohydrate but containing protein and diets lacking protein but containing carbohydrate, larvae consumed a greater proportion of diet containing protein but lacking carbohydrate in the presence of harmane than in its absence. These results are consistent with beta-carboline alkaloid-mediated persistence of serotonin in the brain due to MAO inhibition. Alternatively, these results could reflect alkaloid-mediated peripheral inhibition of sucrose taste receptors influencing ingestive behaviors. That beta-carboline alkaloid ingestion is associated with changes in feeding behavior is consistent with a possible defensive role for these compounds in plant foliage.
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  • 37
    ISSN: 1570-7458
    Keywords: insect endocrinology ; ecdysone ; juvenile hormone ; reproduction ; growth ; inhibin ; oostatin
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    Notes: Abstract The first insect folliculostatic peptide was isolated from vitellogenic ovaries of the mosquitoAedes aegypti. This decapeptide directly inhibits trypsin biosynthesis in the gut, and indirectly ovarian development. The factor was named Trypsin Modulating Oostatic Factor or TMOF by its discoverers. From the fleshfly Neobellieria bullata 2 folliculostatins have been isolated, the hexapeptide Neb-TMOF and the 19-mer Neb-colloostatin. The available data suggest that at least 2 of the 3 folliculostatins originate from matrix (like) proteins present in the ovary, a hitherto unknown source of hormones. Furthermore, one of the folliculostatins (Neb-TMOF) is a potent inhibitor of ecdysone biosynthesis by larval ring glands of fleshflies. The discovery of the dipteran folliculostatins, which do not show any resemblance to inhibins of vertebrates, may significantly contribute to a better understanding of the hormonal control of growth in insects and perhaps, in other animals as well. None of the 3 folliculostatins is blocked at its N- or C-terminus. This, in combination with the pleiotropy of their effects and the narrow species specificity make such peptides prime candidates for, testing their potential in insect pest control by means of molecular biological methods.
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    Entomologia experimentalis et applicata 91 (1999), S. 29-35 
    ISSN: 1570-7458
    Keywords: chemoreception ; deterrents ; Pieris ; Brassicaceae ; cardenolides ; host-plant selection ; evolution
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    Topics: Biology
    Notes: Abstract Pieris butterflies (Lepidoptera: Pieridae) are specialist herbivores of cruciferous plants. They exploit glucosinolates, secondary plant metabolites chemotaxonomically characteristic for this plant family, as token stimuli. In addition to particular glucosinolates, some genera of the Cruciferae contain cardenolides, steroidal allelochemicals that act as potent feeding and oviposition deterrents to several Pieris species. We investigated the sensory mechanisms by which these compounds are perceived in larvae. Pieris caterpillars and many other lepidopterous species are endowed with so-called generalist deterrent receptors, that respond to a broad spectrum of secondary plant substances. In Pieris caterpillars we found a second type of deterrent chemoreceptor in maxillary styloconic taste sensilla. This neuron is very sensitive to cardenolides (threshold 0.1–0.3 μM). The generalist deterrent receptor also responds to these substances but its threshold lies at 50–100× higher concentrations. In behavioural preference experiments Pieris brassicae L. caterpillars preferred cardenolide-treated cabbage leaf discs when confronted with a choice between them and a deterrent substance that does not occur in the Brassicaceae. The cardenolides acted as potent deterrents when offered against untreated cabbage leaf discs. This demonstrates that the balance of activity elicited in the two types of deterrent chemoreceptors determines the behavioural decision.
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    Acta biotheoretica 47 (1999), S. 29-40 
    ISSN: 1572-8358
    Keywords: Sexual selection ; mate selection ; gamete selection ; evolution ; ploidy ; asssortative mating
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    Topics: Biology
    Notes: Abstract Results of an agent-based computer simulation of the evolution of diploid sexual organisms showed that several mate selection strategies confer much higher average fitness to the simulated populations, and higher evolutionary stability to the alleles coding for these strategies, than random mating. Strategies which select for 'good genes' were very successful, and so were strategies based on assortative mating. The results support the hypothesis that mating is not likely to be random in nature and that the most successful mate selection strategies are those based on assortative mating or on advantageous genes.
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    Entomologia experimentalis et applicata 82 (1997), S. 37-44 
    ISSN: 1570-7458
    Keywords: Barbarea vulgaris ; Cruciferae ; Phyllotreta nemorum ; Chrysomelidae ; Alticinae ; flea beetle ; plant defence ; genetics ; sex-linkage ; X- and Y-chromosome ; evolution
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Abstract A polymorphism in host plant exploitation has been discovered in the flea beetle, Phyllotreta nemorum L. (Coleoptera: Chrysomelidae: Alticinae) where one resistant population is able to use Barbarea vulgaris R.Br. ssp. arcuata (Opiz.) Simkovics (Brassicaceae) as a host plant while a susceptible population is not. Crosses (F1, F2, and backcrosses) between the two flea beetle populations were made, and survival of the progeny on B. v. ssp. arcuata was measured. The ability of P. nemorum larvae to survive in this plant species depended on the presence of major, dominant genes (R-genes). The two most abundant R-genes in the resistant flea beetle population were X- and Y-linked, respectively. The use of B. v. ssp. arcuata as a natural host plant by the resistant population of P. nemorum seems to be an extension of the host plant range of the species. The role of sex-linked genes in the evolution of host range is discussed.
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    Entomologia experimentalis et applicata 82 (1997), S. 25-35 
    ISSN: 1570-7458
    Keywords: Barbarea vulgaris ; Cruciferae ; Phyllotreta nemorum ; Chrysomelidae ; Alticinae ; flea beetle ; plant defence ; resistance ; host plant ; variation ; evolution
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    Notes: Abstract Several sorts of variation in the interaction between the insect, Phyllotreta nemorum L. (Coleoptera:Chrysomelidae:Alticinae), and the plant, Barbarea vulgaris R.Br. (Brassicaceae), have been discovered: 1) genetic differences in the levels of defences in the plant, 2) genetic differences in the ability of insects to cope with the plant defences, 3) seasonal variation in levels of defences in the plant, and 4) differences between leaf types in levels of defences. Two plant accessions were suitable for larval development throughout the season while the remaining nine accessions were more or less unsuitable for larvae from the ‘susceptible’ T-population at least at certain times of the year. All accessions were suitable for the ‘resistant’ E-population throughout the year. There was a seasonal variation in levels of defences in some accessions which were unsuitable for the T-population during the summer period when beetles were present, but not during autumn and spring when the beetle were hibernating. Upper (younger) cauline leaves of these accessions had higher levels of defences than lower (older) cauline leaves. The resistant E-population used B. vulgaris as a natural host plant while the susceptible T-population did not. The use of B. vulgaris as a natural host plant by the E-population of P. nemorum seems to be an extension of the host plant range of the species. Variation in plant defences may have facilitated the switch in host plant use by the resistant flea beetle population.
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    Cellular and molecular life sciences 52 (1996), S. 14-24 
    ISSN: 1420-9071
    Keywords: Lycaenidae ; Formicidae ; symbiosis ; mutualism ; parasitism ; communication ; ecology ; evolution
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    Topics: Biology , Medicine
    Notes: Abstract Associations with ants, termed myrmecophily, are widespread in the butterfly family Lycaenidae and range from mere co-existence to more or less specific mutualistic or even parasitic interactions. Secretions of specialized epidermal glands are crucial for mediating the interactions. Transfer of nutrients (carbohydrates, amino acids) from butterfly larvae to ants plays a major role, but manipulative communication with the help of odour signals is also involved. By means of myrmecophily, lycaenid butterflies largely escape ant predation, and certain species gain protection through attendant ants or achieve developmental benefits from ant-attendance. Benefits to the ants range from minimal to substantial food rewards. While most lycaenid species maintain facultative relationships with a variety of ant genera, highly specific and obligatory associations have convergently evolved in a number of butterfly lineages. As a corollary, communication systems are largely unspecific in the former, but may be highly specialized in the latter. The sophisticated communication between obligate myrmecophiles and their host ants is tightly connected with the evolutionary rise of specialized life-cycles and thus is a source of augmenting diversity within the butterflies.
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    Cellular and molecular life sciences 51 (1995), S. 454-464 
    ISSN: 1420-9071
    Keywords: Quantitative genetics ; life history ; evolution ; cladocera ; heritability ; Daphnia ; zooplankton
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    Topics: Biology , Medicine
    Notes: Abstract Quantitative genetic techniques are powerful tools for use in understanding the microevolutionary process. Because of their size, lifespan, and ease of culture, many zooplankton species are ideal for quantitative genetic approaches. As model systems, studies of zooplankton life histories are becoming increasingly used for examination of the central paradigms of evolutionary theory. Two of the fundamental empirical questions that zooplankton quantitative genetics studies can answer are: 1) How much genetic variance exists in natural populations for life history traits? 2) What is the empirical evidence for trade-offs that permeate life history theory based on optimality approaches? A review of existing data onDaphnia indicates substantial genetic variance for body size, clutch size, and age at first reproduction. Average broad-sense heritabilities for these three characters across 19 populations of 6 species are 0.31, 0.31, and 0.34, respectively. Although there is some discrepancy between the two pertinent studies that were designed to decompose the total genetic variance into its additive and non-additive components, a crude average seems to suggest that approximately 60% of the total genetic variance has an additive basis. The existing data are somewhat inconsistent with respect to presence/absence of trade-offs (negative genetic correlations) among life history traits. A composite of the existing data seems to argue against the existence of strong trade-offs between offspring size and offspring number, between present and future reproduction, and between developmental rate and fecundity. However, there is some evidence for a shift toward more negative (less positive) covariances in more stressful environments (e.g., low food). Zooplankton will prove to be very useful in future study in several important areas of research, including the genetics and physiology of aging, the importance of genotype-environment interaction for life history traits, and the evolution of phenotypic plasticity.
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    Cellular and molecular life sciences 52 (1996), S. 503-510 
    ISSN: 1420-9071
    Keywords: Drosophila ; accessory gland ; reproduction ; sexual behavior ; sperm displacement ; evolution
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    Topics: Biology , Medicine
    Notes: Abstract Recent results from biochemical and molecular genetic studies of the accessory gland proteins in maleDrosophila are reviewed. The most prominent feature is the species-specific variability. However, the analysis of the sex peptide inD. melanogaster shows that there is a strong homology in the molecular structure to the closely related sibling species, and that divergence increases with increasing phylogenetic distance. For this reason the sex peptide, after being transferred to the female genital tract during copulation, reduces receptivity and increases oviposition only in virgin females belonging to the same species group and subgroup. Even though studies were hitherto limited to a small number of the secretory components, it is evident that the accessory gland proteins play a key role in reproductive success of the fruit fly by changing female sexual behavior, supporting sperm transfer, storage and displacement. Thus, genes encoding the accessory gland proteins are apparently under strong evolutionary selection.
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    BioMetals 11 (1998), S. 277-295 
    ISSN: 1572-8773
    Keywords: evolution ; classification ; EF-hand ; domain ; homology ; chimera ; congruence ; gene duplication ; gene fusion ; eukaryote ; dendrogram ; calmodulin ; troponin C ; light chain of myosin ; S100 ; parvalbumin ; calcineurin ; recoverin ; calpain ; sorcin ; diacylglycerol ; calbindin ; aequorin ; phospholipase C ; BM-40
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Forty-five distinct subfamilies of EF-hand proteins have been identified. They contain from two to eight EF-hands that are recognizable by amino acid sequence as being statistically similar to other EF-hand domains. All proteins within one subfamily are congruent to one another, i.e. the dendrogram computed from one of the EF-hand domains is similar, within statistical error, to the dendrogram computed from another(s) domain. Thirteen subfamilies - including Calmodulin, Troponin C, Essential light chain, Regulatory light chain - referred to collectively as CTER, are congruent with one another. They appear to have evolved from a single ur-domain by two cycles of gene duplication and fusion. The subfamilies of CTER subsequently evolved by gene duplications and speciations. The remaining 32 subfamilies do not show such general patterns of congruence; however, some - such as S100, intestinal calcium binding protein (calbindin 9kd), and trichohylin - do not form congruent clusters of subfamilies. Nearly all of the domains 1, 3, 5, and 7 are most similar to other ODD domains. Correspondingly the EVEN numbered domains of all 45 subfamilies most closely resemble EVEN domains of other subfamilies. Many sequence and chem-ical characteristics do not show systemic trends by subfamily or species of host organisms; such homoplasy is widespread. Eighteen of the subfamilies are heterochimeric; in addition to multiple EF-hands they contain domains of other evolutionary origins.© Kluwer Academic Publishers
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    Minds and machines 6 (1996), S. 463-480 
    ISSN: 1572-8641
    Keywords: Human reasoning ; evolution ; deontic reasoning ; transitive reasoning ; non-human primates ; neocortical ratio ; dominance hierarchy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Philosophy
    Notes: Abstract Research from ethology and evolutionary biology indicates the following about the evolution of reasoning capacity. First, solving problems of social competition and cooperation have direct impact on survival rates and reproductive success. Second, the social structure that evolved from this pressure is the dominance hierarchy. Third, primates that live in large groups with complex dominance hierarchies also show greater neocortical development, and concomitantly greater cognitive capacity. These facts suggest that the necessity of reasoning effectively about dominance hierarchies left an indelible mark on primate reasoning architectures, including that of humans. In order to survive in a dominance hierarchy, an individual must be capable of (a) making rank discriminations, (b) recognizing what is forbidden and what is permitted based one's rank, and (c) deciding whether to engage in or refriin from activities that will allow one to move up in rank. The first problem is closely tied to the capacity for transitive reasoning, while the second and third are intimately related to the capacity for deontic reasoning. I argue that the human capacity for these types of reasoning have evolutionary roots that reach deeper into our ancestral past than the emergence of the hominid line, and the operation of these evolutionarily primitive reasoning systems can be seen in the development of human reasoning and domain-specific effects in adult reasoning.
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    Minds and machines 9 (1999), S. 309-346 
    ISSN: 1572-8641
    Keywords: language ; grammar ; syntax ; semantics ; evolution ; emergence ; brain size
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    Topics: Computer Science , Philosophy
    Notes: Abstract It is commonly argued that the rules of language, as distinct from its semantic features, are the characteristics which most clearly distinguish language from the communication systems of other species. A number of linguists (e.g., Chomsky 1972, 1980; Pinker 1994) have suggested that the universal features of grammar (UG) are unique human adaptations showing no evolutionary continuities with any other species. However, recent summaries of the substantive features of UG are quite remarkable in the very general nature of the features proposed. While the syntax of any given language can be quite complex, the specific rules vary so much between languages that the truly universal (i.e. innate) aspects of grammar are not complex at all. In fact, these features most closely resemble a set of general descriptions of our richly complex semantic cognition, and not a list of specific rules. General principles of the evolutionary process suggest that syntax is more properly understood as an emergent characteristic of the explosion of semantic complexity that occurred during hominid evolution. It is argued that grammatical rules used in given languages are likely to be simply conventionalized, invented features of language, and not the result of an innate, grammar-specific module. The grammatical and syntactic regularities that are found across languages occur simply because all languages attempt to communicate the same sorts of semantic information.
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    Journal for general philosophy of science 30 (1999), S. 37-58 
    ISSN: 1572-8587
    Keywords: protein ; experimentation ; conceptual variation and selection ; evolution ; Mulder ; Liebig ; Pflüger ; Nägeli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Philosophy , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Abstract A philosophically comprehended account is given of the genesis and evolution of the concept of protein. Characteristic of this development were not shifts in theory in response to new experimental data, but shifts in the range of questions that the available experimental resources were fit to cope with effectively. Apart from explanatory success with regard to its own range of questions, various other selecting factors acted on a conceptual variant, some stemming from a competing set of research questions, others from an altogether different field of inquiry, and still others from the external environment. These results are best explained on, hence support, an evolutionary model of the progress of experimental investigation, whose outlines are briefly discussed.
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    Minds and machines 6 (1996), S. 481-505 
    ISSN: 1572-8641
    Keywords: Neuroscience ; evolutionary psychology ; interfield theory ; evolution ; teleology ; function ; functionalism ; brain mapping ; language processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Philosophy
    Notes: Abstract The idea of integrating evolutionary biology and psychology has great promise, but one that will be compromised if psychological functions are conceived too abstractly and neuroscience is not allowed to play a contructive role. We argue that the proper integration of neuroscience, psychology, and evolutionary biology requires a telelogical as opposed to a merely componential analysis of function. A teleological analysis is required in neuroscience itself; we point to traditional and curent research methods in neuroscience, which make critical use of distinctly teleological functional considerations in brain cartography. Only by invoking teleological criteria can researchers distinguish the fruitful ways of identifying brain components from the myriad of possible ways. One likely reason for reluctance to turn to neuroscience is fear of reduction, but we argue that, in the context of a teleological perspective on function, this concern is misplaced. Adducing such theoretical considerations as top-down and bottom-up constraints on neuroscientific and psychological models, as well as existing cases of productive, multidisciplinary cooperation, we argue that integration of neuroscience into psychology and evolutionary biology is likely to be mutually beneficial. We also show how it can be accommodated methodologically within the framework of an interfield theory.
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    Coral reefs 17 (1998), S. 155-168 
    ISSN: 1432-0975
    Keywords: Key words Atoll hydrodynamics ; Lagoon ciculation ; Wave transformation ; Currents ; Physical processes
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    Topics: Biology , Geosciences
    Notes: Abstract  Detailed measurements of water levels, and tide and wave-induced currents were undertaken to examine physical processes and their relationship with morphology in the Cocos (Keeling) Islands, a medium sized atoll in the Indian Ocean. Results indicate that the atoll structure controls both lagoon circulation and the spatial pattern of energy distribution. Lagoon circulation is tide dominated (currents 16–31 cms-1) with flushing (2–5 days) of the lagoon occurring through the deep leeward passages. Wave- and tide-driven unidirectional flows through shallow passages (26–65 cms-1) are important mechanisms of ocean to lagoon water exchange and contribute up to 24% of the lagoon neap tide prism. Reef flats are dominated by wave energy (maximum velocity 140 cms-1, east) with measurements of the attenuation of wave energy between reef flats and shallow lagoon (80–90%) conforming to measurements from fringing and barrier reefs. Spectral analysis shows that the characteristics of wave energy vary on different sectors of the atoll, with gravity wave energy dominating the east, and infragravity wave energy dominating the southern reef flat and passages. Wave setup at the reef crest is considered to be responsible for an identified 0.1 m higher water level in the southern as opposed to eastern and northern atoll, which promotes higher reef flat growth. Transmission of gravity waves across reef flats requires threshold water depths of 0.65 (east) and 0.70 m (south). The higher southern reef is an effective filter of gravity wave energy for most tidal elevations. Differences in the type and magnitude of physical processes within the atoll are discussed with relation to geomorphic development on Cocos.
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    European journal of nutrition 36 (1997), S. 205-213 
    ISSN: 1436-6215
    Keywords: Frying ; growth ; liver lipids ; lipaemia ; olive oil ; pregnancy ; rat ; Fritierung ; Wachstum ; Leberfett ; Olivenöl ; Schwangerschaft ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Um Informartionen über den Zusammenhang zwischen der Aufnahme von Fett, das zum Fritieren benutzt wurde, und dem Fetthaushalt während Perioden starken Körperaufbaus zu haben, wurde der Einfluß der Aufnahme von frischem Olivenöl (Gehalt polarer Verbindungen, 2 %; Ölsäure 78,9 mg/100 mg Öl, und Linolsäure 7 mg/100 mg Öl) und von Olivenöl, das 15 mal in Folge für das Fritieren von Kartoffeln benutzt worden war (Gehalt polarer Verbindungen 9 %; Ölsäure 75,8 mg/100mg Öl und Linolsäure 6,2 mg/100mg Öl) während der Gravidität, untersucht. Dazu wurden trächtige Wistar Ratten in zwei Gruppen geteilt, die beide eine isokalorische Diät bekamen, deren Fettanteil 15 % von frischem (unbenutztem) (P1) bzw. fritiertem (benutztem) (P2) Olivenöl stammte mit nicht trächtigen Ratten verglichen. Die Gravidität erhöhte (p〈0,01) die Futteraufnahme, das Körpergewicht, die Gewichtszunahmen und die Futterverwertung. Die Ölqualität beeinflußte dagegen diese Parameter nicht. Während der Gravidität stiegen die Serumwerte der Triglyceride (TG) (p〈0,01) und des Cholesterins (TC) (p〈0,05) an, während die der Phosphatide (PH) sanken (p〈0,01). Ein signifikanter Effekt der Ölqualität und eine Wechselwirkung zwischen Gravidität und Öl wurde für TG und PH festgestellt. Das Gewicht und der Fettgehalt der Leber der trächtigen Ratten stiegen signifikant an (p.〈0.05), Leber TC, TG und PH stiegen während der Gravidität (ungefähr um das 3-fache der Ausgangswerte), aber es traten keine signifikanten Unterschiede zwischen der Aufnahme von benutztem und nicht benutztem Öl (P2 vs P1) auf. Die Ergebnisse zeigen, daß die Aufnahme von leicht verdorbenem Olivenöl als alleinige Fettquelle der Nahrung keine besonderen Folgen für die Gravidität hat, was die Gewichtszunahme der Mütter und der Feten, die Lipämie und die Zusammensetzung des Leberfetts betrifft.
    Notes: Summary The effect of the consumption of unused olive oil (polar content, 2 %; oleic acid, 78.9 mg/100 mg oil, and linoleic acid 7 mg/100 mg oil) and olive oil used discontinuously for frying potatoes 15 times (polar content, 9 %; oleic acid, 75.8 mg/100 mg oil and linoleic acid 6.2 mg/100 mg oil) was studied in pregnant rats with the aim of better understanding the relationship between the consumption of fat used in frying and lipid metabolism during periods of intense anabolism. Trials were performed in pregnant Wistar rats, divided into 2 groups and fed isocaloric diets in which the fat content (15 % wt/wt) consisted of unused olive oil (P1) or oil previously used for frying (P2), and the results were compared with those of nonpregnant rats fed unused olive oil (NP1) and olive oil used for frying (NP2). Pregnancy increased (p〈0.01) food intake, body weight, weight gain, and food efficiency ratio (P2 vs NP2 and P1 vs NP1, respectively), but the treatment of oil included in the diets did not alter these parameters. Gestation significantly increased the serum triglyceride (TG) (p〈0.01) and total cholesterol (TC) (p〈0.05) concentrations and diminished that of phospholipids (PH) (p〈0.01). A significant effect of the type of oil consumed and a pregnancy x oil interaction on Tg and PH levels was observed. The weight of the liver and its fat content increased significantly (p〈0.05) as a result of pregnancy. Liver TC, TG, and PH increased (approximately 3 times the original values) during gestation, but no significant differences due to the intake of used or unused oil (P2 vs P1) were observed. The results indicate that the consumption of moderately altered olive oil, as the sole source of fat, does not alter the effect of pregnancy on the mothers' weight gain, lipaemia, and hepatic fat composition to any important degree.
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  • 52
    ISSN: 1436-6215
    Keywords: Body composition ; fat ; growth ; somatotropin ; pig ; Körperzusammensetzung ; Fett ; Wachstum ; Somatotropin -Schwein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung An 78 männlichen Kastraten und weiblichen Schweinen der Kreuzung Pietrain x (Deutsches Edelschwein x Deutsche Landrasse) wurden Untersuchungen zum Einfluß einer porcinen Somatotropin (pST)-Behandlung während des Wachstums auf die chemische Körperzusammensetzung der Tiere, das Adipozyten-Wachstum und das Fettsäure-Profil des Rückenspecks untersucht. Die intramuskulären Injektionen (1 oder 3 mg pST) wurden täglich ab durchschnittlich 65 kg Lebendgewicht bis zum Schlachten verabreicht. Nach der pST-Behandlung wurden bei den Kastraten in allen untersuchten Merkmalen signifikante Unterschiede festgestellt, während sich bei den weiblichen Tieren nur leichte Reaktionen zeigten. Das pST verursachte eine Zunahme des Wasserund Proteingehaltes bei gleichzeitiger Reduzierung des Lipidgehaltes besonders in den fettreichen Körperteilen der Tiere. Im Rückenspeck war der Gehalt an ungesättigten Fettsäuren erhöht und der Fettzelldurchmesser erniedrigt.
    Notes: Summary Seventy eight growing-finishing pigs (male castrates and females) of the cross-breed Pietrain x (Large White x German Landrace) were used to investigate the effects of pST treatment on the chemical composition of the body, the growth of adipocytes, and the fatty acid profile of the backfat. Intramuscular injections (1 or 3 mg pST) were administered daily from an average weight of 65 kg up to slaughter. After pST treatment significant changes in all studied characteristics were observed in barrows, whereas the females exhibited very small responses. The pST caused an increase of water and protein contents and a simultaneous decrease of lipid content especially in body parts rich in fat. Furthermore, the proportion of unsaturated fatty acids increased and the fat cell diameter decreased in the backfat.
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  • 53
    ISSN: 1573-0417
    Keywords: colonization ; evolution ; lakes ; Norway ; deglaciation ; land uplift ; invertebrates ; Chironomidae ; Porifera ; Bryozoa ; diatoms ; Charophyta ; tsunami
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Invertebrate colonization of lakes following the uplift of land from the sea was studied in four lakes, currently situated between 39 and 24 m a.s.l., on the central Norwegian coast. The lakes were isolated from the sea between 9500 and 7700 years B.P. Animal and algal remains picked from core samples showed that the first colonizers preserved as fossils were usually members of the Chironomidae, Daphnidae/Chydoridae, Acarina, Porifera (Ephydatia mülleri and Spongilla lacustris), Bryozoa (Cristatella mucedo and Plumatella spp.) and Charophyta (Chara sp.). Of the chironomids, the genus Chironomus was present in the oldest lacustrine layers of all four lakes, but other genera recorded at the marine/lacustrine boundary were Dicrotendipes, Procladius (?), Einfeldia, Microtendipes, and Glyptotendipes. Remains of the caddis fly family Limnephilidae were also present in the earliest lacustrine sediments in Kvennavatnet and Kvernavatnet. The oldest invertebrate fauna is typical for mesotrophic lakes. However, chironomids and mites have been present in this area from at least about 10 500 years B.P. A diverse chironomid community was established between 300 and 800 years after isolation from the sea at Kvernavatnet on the island of Hitra, while only between 80 and 120 years passed before a comparably diverse community developed at Kvennavatnet on the mainland coast. A similar development of the invertebrate fauna occurred in Kvennavatnet, Kvernavatnet and Storkuvatnet. However, Litjvatnet deviates greatly from the ‘normal’ pattern because a tsunami disturbed the bottom sediments and fauna. The tsunami, a gigantic sea wave, was caused by a submarine slide from the Norwegian continental slope. It reached Litjvatnet, today located 24 m a.s.l., but was not traced in Storkuvatnet at 30 m a.s.l. This event happened about 7200 years B.P.
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  • 54
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    Journal of productivity analysis 8 (1997), S. 293-310 
    ISSN: 1573-0441
    Keywords: growth ; USagriculture ; externalities ; spill-overs ; public R and D
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract Growth in U.S. agriculture is linked to the non-farm economy through domestic terms of trade and factor market adjustments. With almost stable input growth, the relatively large contributions from growth in Total Factor Productivity (TFP) are passed on to intermediate and final consumers in the form of declining real prices for primary farm products. The resulting net growth in the real value of farm output (GDP) is relatively low (0.25% per annum). The decomposition of TFP suggests that public agricultural stock of knowledge and infrastructure are “robustly” associated with TFP growth, while spill-overs from private agricultural and economy wide research and development (R and D) are positive but, relatively small.
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  • 55
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    Antonie van Leeuwenhoek 71 (1997), S. 159-178 
    ISSN: 1572-9699
    Keywords: aromatic pathways ; chlorobenzenes ; evolution ; genes ; plasmids ; pseudomonas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chlorobenzenes are substrates not easily metabolized by existing bacteria in the environment. Specific strains, however, have been isolated from polluted environments or in laboratory selection procedures that use chlorobenzenes as their sole carbon and energy source. Genetic analysis indicated that these bacteria have acquired a novel combination of previously existing genes. One of these gene clusters contains the genes for an aromatic ring dioxy-genase and a dihydrodiol dehydrogenase. The other contains the genes for a chlorocatechol oxidative pathway. Comparison of such gene clusters with those from other aromatics degrading bacteria reveals that this process of recombining or assembly of existing genetic material must have occurred in many of them. Similarities of gene functions between pathways suggest that incorporation of existing genetic material has been the most important mechanism of expanding a metabolic pathway. Only in a few cases a horizontal expansion, that is acqui sition of gene functions to accomodate a wider range of substrates which are then all transformed in one central pathway, is observed on the genetic level. Evidence is presented indicating that the assembly process may trigger a faster divergence of nearby gene sequences. Further ‘fine-tuning’, for example by developing a proper regulation, is then the next step in the adaptation.
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  • 56
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    Antonie van Leeuwenhoek 71 (1997), S. 265-270 
    ISSN: 1572-9699
    Keywords: bacteria ; DNA ; evolution ; genome ; RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This review examines evolution of bacterial genomes with an emphasis on RNA based life, the transition to functional DNA and small evolving genomes (possibly plasmids) that led to larger, functional bacterial genomes.
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  • 57
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    Antonie van Leeuwenhoek 71 (1997), S. 257-263 
    ISSN: 1572-9699
    Keywords: bacteria ; energy ; evolution ; genome ; metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This article examines the relationship between (or dependence of) bacterial evolution in prokaryotes and metabolism, and the changing physical-chemical conditions present during early evolution.
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  • 58
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    Antonie van Leeuwenhoek 71 (1997), S. 363-368 
    ISSN: 1572-9699
    Keywords: assembly ; anode ; bacteria ; cathode ; DNA ; evolution ; genetics ; molecular ; surfaces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Molecular evolution is examined in bacteria with an emphasis on mineral surfaces, membranes, cathodes and anodes. In early molecular evolution, cathode-anode system may have been naturally occurring on a nm to µm scale. Secondly, the cathode-anode system could have been separated by a primitive, permeable lipid or microsphere on a mineral surface, that was a precursor of a more advanced membrane with a charge differential on either side of the membrane. These aspects will be considered from a theoretical evolutionary perspective.
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  • 59
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    Antonie van Leeuwenhoek 72 (1997), S. 251-259 
    ISSN: 1572-9699
    Keywords: bacteria ; catalysis ; DNA ; enzyme ; evolution ; microorganisms ; optimization ; RNA ; time
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microbial populations (and life) not only evolve, they optimize. The transition from a random, unorganized, lifeless Earth to the present situation, where the Earth is virtually covered with nucleic acids and diverse and complex species, required numerous molecular changes and the integration of metabolic pathways over billions of years. Primitive prokaryotic life was dependent on and constrained by the physical-chemical conditions on the Earth, while slowly reshaping conditions present. In this review, molecular evolution and molecular optimization are examined with an emphasis on the order in which evolutionary events occurred.
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  • 60
    ISSN: 1572-977X
    Keywords: Avicennia alba ; growth ; internodal sequences ; mangrove age determination ; Rhizophora apiculata ; Sonneratia caseolaris ; Thailand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence of periodic changes in the length of the internodes of the seedlings of SE Asian mangrove species (Rhizophora apiculata, Avicennia alba, and Sonneratia caseolaris) growing in the Pak Phanang estuary (Nakhon Si Thammarat, SE Thailand) was confirmed. The annual nature of these periodic changes was verified through comparisons with the observed average number of internodes produced by plants tagged for one year. The cycles in the sequence of internodal length of the seedlings had an average (± SE) period of 16.0 ± 0.78 internodes for Avicennia alba, 25.0 ± 1.2 internodes for Sonneratia caseolaris, and 8.29 ± 0.40 internodes for Rhizophora apiculata. These values are remarkably close to, and not significantly different (t‐test, P 〉 0.05) from the average (± SE) annual number of internodes of 17.6 ± 0.8 for Avicennia alba, 28.8 ± 2.1 for Sonneratia caseolaris, and 8.03 ± 0.36 for Rhizophora apiculata produced in a year by tagged plants. In addition, the average (± SE) growth of the tagged plants was 0.39 ± 0.018 cm day-1 for Avicennia alba, 0.24 ± 0.015 cm day-1 for Sonneratia caseolaris, and 0.091 ± 0.0036 cm day-1 for Rhizophora apiculata. These estimates were very close to those (0.45 ± 0.06 cm day-1 for Avicennia alba, 0.32 ± 0.01 cm day-1 for Sonneratia caseolaris, and 0.13 ± 0.005 cm day-1 for Rhizophora apiculata) derived as the product of the mean number of internodes produced per day, calculated from the inferred number of internodes produced per year, and the mean length of the internodes of the plants. These findings indicate that the use of regular, annual changes in the length of the internodes of mangrove seedlings can be used reliably to determine their age and to estimate their growth rate.
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  • 61
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    Journal of the history of biology 32 (1999), S. 343-383 
    ISSN: 1573-0387
    Keywords: agnosticism ; Darwinian ; evolution ; materialism ; Malthusian ; nebular hypothesis ; popularization ; professionalization ; transitional forms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , History
    Notes: Abstract Robert Chambers and Thomas Henry Huxley helped popularize science by writing for general interest publications when science was becoming increasingly professionalized. A non-professional, Chambers used his family-owned Chambers' Edinburgh Journal to report on scientific discoveries, giving his audience access to ideas that were only available to scientists who regularly attended professional meetings or read published transactions of such forums. He had no formal training in the sciences and little interest in advancing the professional status of scientists; his course of action was determined by his disability and interest in scientific phenomena. His skillful reporting enabled readers to learn how the ideas that flowed from scientific innovation affected their lives, and his series of article in the Journal presenting his rudimentary ideas on evolution, served as a prelude to his important popular work, Vestiges of the Natural History of Creation. Huxley, an example of the new professional class of scientists, defended science and evolution from attacks by religious spokesmen and other opponents of evolution, informing the British public about science through his lectures and articles in such publications as Nineteenth Century. He understood that by popularizing scientific information, he could effectively challenge the old Tory establishment -- with its orthodox religious and political views -- and promote the ideas of the new class of professional scientists. In attempting to transform British society, he frequently came in conflict with theologians and others on issues in which science and religion seemed to contradict each other but refused to discuss matters of science with non-professionals like Chambers, whose popular writing struck a more resonant chord with working class readers.
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  • 62
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    De economist 144 (1996), S. 397-428 
    ISSN: 1572-9982
    Keywords: conventions ; institutions ; game theory ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Summary This survey article starts with a game-theory interpretation of coordination problems that occur in an economy. Three types of games are discussed in which the degree of coordination versus conflict varies. It is shown that game-theoretic techniques for equilibrium selection or securing the highest pay-off outcome do not always suffice, which raises the need for exogenous information. Norms, such as conventions and institutions, may provide this information. The emergence and persistence of norms as well as the relationship between the type of game and the type of norm are discussed. After a discussion on conventions and rationality, some notions from Institutional Economics are introduced, in which institutions are explained as a way to deal with limited and costly information. Some applications are given in the last section.
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  • 63
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    Insectes sociaux 42 (1995), S. 57-69 
    ISSN: 1420-9098
    Keywords: Hindgut ; alkalinity ; evolution ; symbionts ; gut morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The pH of the gut contents was measured in 52 species of higher termites (Termitidae), representing 36 genera in all four subfamilies. A statistically significant trend was shown from lower termites with low mean gut pH through to the Termitinae with higher mean gut pHs. Elevation of the pH occurred principally in the first and third proctodaeal segments, reaching values as high as 10.5 in 8 soil-feeding genera and 1 wood-feeding genus of Termitinae. Elevation of gut pH within the Termitidae appears to be independent of the general nature of the feeding substrate.
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  • 64
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    Insectes sociaux 43 (1996), S. 375-389 
    ISSN: 1420-9098
    Keywords: Nasutitermitinae ; Subulitermes ; Coatitermes ; Velocitermes ; evolution ; phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The developmental pathways of the neuter castes were studied in three species of Nasutitermitinae from central Panama. The humivorousSubulitermes denisae andCoatitermes clevelandi display several primitive traits: absence of sex dimorphism, representation of both sexes among workers and soldiers, and occurrence of successive worker instars. The litter-dwellingVelocitermes barrocoloradensis has a more complex caste system: female larvae are larger than males and give rise to the large workers, which constitute the bulk of the work force; male larvae proceed to soldiers through a small worker or a special larval instar. The resulting soldier caste is polymorphic. These results support previously formulated hypotheses regarding a link between humivorous diet and reduced polymorphism on the one hand, and between forest-floor foraging and large continuous size variation among soldiers on the other. Whereas the caste systems ofSubulitermes andCoatitermes probably represent a primitive condition,Velocitermes shares derived traits withNasutitermes and the other fully nasute genera previously studied. I therefore hypothesize that ancestors with these advanced features may have spread from the neotropics and be at the origin of most nasute genera, including humivorous taxa, present in other regions.
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  • 65
    ISSN: 1420-9098
    Keywords: Formicidae ; Leptothoracini ; Tetramoriini ; internal transcribed spacer ; social parasitism ; evolution ; phylogenetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A fragment of the internal transcribed spacer (ITS-1) adjacent to the 5.8S rRNA gene of 20 myrmicine ant species was sequenced. Sequence comparisons were carried out between 11 species of the tribe Leptothoracini, five species of the tribe Tetramoriini, three species of the tribe Solenopsidini and one species of the tribe Myrmicini. Additionally, the formicine antCamponotus ligniperda (tribe Camponotini) was analyzed as an outgroup species. Among all investigated species, the fragment had a variable length of ≈ 230–380 bp with only a few conserved sequence elements. The sequences of this fragment were perfectly identical within four palearctic populations ofLeptothorax acervorum indicating that intraspecific variation is rather low. Within the species of Tetramoriini (includingAnergates atratulus) 94.1% of sequence positions were identical, 95.6% within the species of theLeptothorax s.str.-group and 64.6% within the species of theMyrafant-group. Phylogenetic analysis reveals that the social parasitesHarpagoxenus sublaevis, Doronomyrmex goesswaldi, D. kutteri andD. pacis, Chalepoxenus muellerianus as well asStrongylognathus alpinus andTeleutomyrmex schneideri are most closely related to the groups of their respective host species, which generally confirms the taxonomical classifications of the subfamily Myrmicinae based on morphological criteria. The taxonomical positions of the speciesA. atratulus has as yet been uncertain, however, sequence comparison of the ITS-1 fragment leads to the conclusion thatA. atratulus rather belongs to the tribe Tetramoriini than to the Solenopsidini.
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  • 66
    ISSN: 1423-0445
    Keywords: sex pheromone ; synergist ; antagonist ; mate recognition ; reproductive isolation ; chemotaxonomoy ; phylogeny ; evolution ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The geometric isomers (E,E)-, (E,Z)-, (Z,E)-, and (Z,Z)-8,10-dodecadien-1-yl acetate were identified as sex pheromone components or sex attractants in the tribes Eucosmini and Grapholitini of the tortricid subfamily Olethreutinae. Species belonging to the more ancestral Tortricinae were not attracted. Each one isomer was behaviourally active in males ofCydia andGrapholita (Grapholitini), either as main pheromone compound, attraction synergist or attraction inhibitor. Their reciprocal attractive/antagonistic activity in a number of species enables specific communication with these four compounds.Pammene, as well as otherGrapholita andCydia responded to the monoenic 8- or 10-dodecen-1-yl acetates. Of the tribes Olethreutini and Eucosmini,Hedya, Epiblema, Eucosma, andNotocelia trimaculana were also attracted to 8,10-dodecadien-1-yl acetates, but several otherNotocelia to 10,12-tetradecadien-1-yl acetates. The female sex pheromones ofC. fagiglandana, C. pyrivora, C. splendana, Epiblema foenella andNotocelia roborana were identified. (E,E)- and (E,Z)-8,10-dodecadien-1-yl acetate are producedvia a commonE9 desaturation pathway inC. splendana. CallingC. nigricana andC. fagiglandana females are attracted to wingfanning males.
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  • 67
    ISSN: 1420-9071
    Keywords: Alternating high magnetic fields ; eddy current ; magnetic flux concentration ; Caenorhabditis elegans ; growth ; reproduction ; behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Here we describe a device for testing possible influences of high magnetic fields on biological processes, by which alternating-current magnetic stimuli as high as 1.7 T can be administered. Experiments with a simple multicellular organism, the nematodeCaenorhabditis elegans, revealed that intermittent exposure to the magnetic fields modestly inhibited the animal's reproduction as well as its post-embryonic development, and caused a marked but transient derangement in its locomotory behavior. Available evidence indicates that alternating high magnetic fields can elicit both chronic and acute biological effects, but that the effects may be well tolerated or compensated for by the living organism.
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    Immunogenetics 49 (1999), S. 865-871 
    ISSN: 1432-1211
    Keywords: Key words Orangutan ; MHC class I ; HLA-C ; natural killer cells ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  HLA-B and C are related class I genes which are believed to have arisen by duplication of a common ancestor. Previous study showed the presence of orthologues for both HLA-B and C in African apes but only for HLA-B in Asian apes. These observations suggested that the primate C locus evolved subsequent to the divergence of the Pongidae and Hominidae. From an analysis of orangutan Tengku two HLA-C-like alleles (Popy C*0101 and Popy C*0201) were defined as well as three HLA-B-like (Popy-B) alleles. By contrast, no Popy-C alleles were obtained from orangutan Hati, although three Popy-B alleles were defined. Thus an HLA-C-like locus exists in the orangutan (as well as a duplicated B locus), implying that the primate C locus evolved prior to the divergence of the Pongidae and Hominidae and is at least 12–13 million years old. Uncertain is whether all orangutan MHC haplotypes contain a C locus, as the failure to find C alleles in some individuals could be due to a mispairing of HLA-C-specific primers with certain Popy-C alleles. These results raise the possibilities that other primate species have a C locus and that the regulation of natural killer cells by C allotypes evolved earlier in primate evolution than has been thought.
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  • 69
    ISSN: 1432-1939
    Keywords: Key words Agrostis canina ; CO2 vents ; photosynthesis ; lignification ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The aim of this study was to characterise growth and photosynthetic capacity in plants adapted to long-term contrasting atmospheric CO2 concentrations (C a). Seeds of Agrostis canina L. ssp. monteluccii were collected from a natural CO2 transect in central-western Italy and plants grown in controlled environment chambers at both ambient and elevated CO2 (350 and 700 μmol mol−1) in nutrient-rich soil. Seasonal mean C a at the source of the plant material ranged from 610 to 451 μmol CO2 mol−1, derived from C4 leaf stable carbon isotope discrimination (δ13C). Under chamber conditions, CO2 enrichment stimulated the growth of all populations. However, plants originating from elevated C a exhibited higher initial relative growth rates (RGRs) irrespective of chamber CO2 concentrations and a positive relationship was found between RGR and C a at the seed source. Seed weight was positively correlated with C a, but differences in seed weight were found to explain no more than 34% of the variation in RGRs at elevated CO2. Longer-term experiments (over 98 days) on two populations originating from the extremes of the transect (451 and 610 μmol CO2 mol−1) indicated that differences in growth between populations were maintained when plants were grown at both 350 and 700 μmol CO2 mol−1. Analysis of leaf material revealed an increase in the cell wall fraction (CWF) in plants grown at elevated CO2, with plants originating from high C a exhibiting constitutively lower levels but a variable response in terms of the degree of lignification. In vivo gas exchange measurements revealed no significant differences in light and CO2 saturated rates of photosynthesis and carboxylation efficiency between populations or with CO2 treatment. Moreover, SDS-PAGE/ LISA quantification of leaf ribulose bisphosphate carboxylase/oxygenase (Rubisco) showed no difference in Rubisco content between populations or CO2 treatments. These findings suggest that long-term adaptation to growth at elevated CO2 may be associated with a potential for increased growth, but this does not appear to be linked with differences in the intrinsic capacity for photosynthesis.
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    Journal of population economics 9 (1996), S. 415-428 
    ISSN: 1432-1475
    Keywords: H42 ; J 13 ; O 11 ; Fertility ; growth ; public education and health
    Source: Springer Online Journal Archives 1860-2000
    Topics: Sociology , Economics
    Notes: Abstract This paper considers the implications of the financing of government services to children when fertility decisions are endogenously determined. In particular, it is shown that when the services are financed by taxation, the equilibrium outcome is biased away from the socially preferred result. The bias results in higher fertility rates and lower economic growth rates than the efficient social optimum. This arises because each household internalizes the benefits, but not the costs of the tax-financed services. We consider alternative methods of financing the public provision of services and find that a combination of taxation and vouchers can eliminate the bias in the equilibrium outcome.
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  • 71
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    Journal of population economics 11 (1998), S. 273-291 
    ISSN: 1432-1475
    Keywords: JEL classification: F22 ; O3 ; J61 ; Key words: Immigration ; assimilation ; growth ; diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Sociology , Economics
    Notes: Abstract. This paper analyzes the welfare effects of immigration and its subsequent effect on ethnic diversity in a model featuring human capital spillovers which depend on the degree of ethnic heterogeneity, variation rates of time preference across individuals and endogenous levels of immigration and assimilation. In the model, an increase in ethnic diversity reduces the spillovers effect for the majority. Nonetheless, immigration can be welfare improving for the majority ethnic group even if it increases the degree of diversity as long as it raises the average human capital level and/or growth rate by increasing the proportion of people with low rates of time preference. However, if an economy is too homogenous, it will not be able to attract immigrants. Finally, if the level of immigration is not too high, then immigration also raises the net benefits to assimilation which leads to a more homogenous economy.
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  • 72
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    Journal of population economics 11 (1998), S. 517-534 
    ISSN: 1432-1475
    Keywords: Key words: Fertility ; mortality ; growth ; JEL classification: J13 ; O41
    Source: Springer Online Journal Archives 1860-2000
    Topics: Sociology , Economics
    Notes: Abstract. Economic and demographic outcomes are determined jointly in a choice-theoretic model of fertility, mortality and capital accumulation. There is an endogenous population of reproductive agents who belong to dynastic families of overlapping generations connected through altruism. In addition to choosing savings and births, parents may reduce (infant) deaths by incurring expenditures on health-care which is also provided by the government. A generalised production technology accounts for long-run endogenous growth with short-run transitional dynamics. The analysis yields testable time series and cross-section implications which accord with the empirical evidence on the relationship between demography and development.
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  • 73
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    Journal of population economics 9 (1996), S. 415-428 
    ISSN: 1432-1475
    Keywords: Key words: Fertility ; growth ; public education and health
    Source: Springer Online Journal Archives 1860-2000
    Topics: Sociology , Economics
    Notes: Abstract. This paper considers the implications of the financing of government services to children when fertility decisions are endogenously determined. In particular, it is shown that when the services are financed by taxation, the equilibrium outcome is biased away from the socially preferred result. The bias results in higher fertility rates and lower economic growth rates than the efficient social optimum. This arises because each household internalizes the benefits, but not the costs of the tax-financed services. We consider alternative methods of financing the public provision of services and find that a combination of taxation and vouchers can eliminate the bias in the equilibrium outcome. JEL classification: H42, J13, O11
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  • 74
    ISSN: 1432-072X
    Keywords: Key words Protein phosphorylation ; ATP-Pi exchange ; Light-harvesting complexes ; Reaction center ; Photosynthesis ; Photomorphogenesis ; Membrane ; growth ; Polypeptide insertion ; Phospho-amino esters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Membranes of Rhodobacter capsulatus strain U43 (pTX35) showed qualitatively very similar phosphorylation patterns under in vitro and in vivo conditions. In vitro, it was irrelevant whether the phosphate source was orthophosphate or ATP. Inhibitors of electron transport did not inhibit light-harvesting complex I (LHIα) (B870) polypeptide phosphorylation, except for o-phenanthroline, which was strongly inhibitory. Redox conditions regulated the amount of protein phosphorylated; external redox potentials between +200 and +300 mV promoted the reaction. Phosphorylation was inhibited by uncouplers such as carbonyl cyanide m-chlorophenyl hydrazone and nigericin plus valinomycin plus potassium ions. Inhibitors of the H+-ATPase were also inhibitory when the phosphate source was [32P]Pi or [γ-32P]ATP. From these results, it was concluded that an operative reaction center, a coupled membrane, and external redox potentials higher than +200 mV are required for optimum LHIα phosphorylation. We also demonstrated that phosphorylation of LHIα polypeptide occurs before insertion into the membrane and that phosphate is preferentially incorporated into specific domains within the cytoplasmic membrane. Intracytoplasmic membranes, identified here as light membranes, were found to contain a dephosphorylated LHIα polypeptide.
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  • 75
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    Invertebrate neuroscience 1 (1995), S. 199-206 
    ISSN: 1439-1104
    Keywords: phospholipase C ; inositol phosphate ; invertebrate vision ; olfaction ; growth ; differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: abstract Phosphatidylinositol-specific phospholipase C (PLC) is a family of enzymes that occupy a pivotal role in one of the largest classes of cellular signaling pathways known. Mammalian PLC enzymes have been divided into four major classes and a variety of subclasses based on their structural characteristics and immunological differences. There have been five invertebrate PLC-encoding genes cloned thus far and these fall within three of the four major classes used in categorizing mammalian PLC. Four of these invertebrate genes have been cloned fromDrosophila melanogaster and one is fromArtemia, a brine shrimp. Structural characteristics of the invertebrate enzymes include the presence of highly conserved Box X and Box Y domains found in major types of mammalian PLC as well as novel features. Two of the invertebrate PLC genes encode multiple splice-variant subtypes which is a newly emerging level of diversity observed in mammalian enzymes. Studies of the invertebrate PLCs have contributed to the identification of the physiological functions of individual isozymes. These identified roles include cellular processes such as phototransduction, olfaction, cell growth and differentiation.
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  • 76
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    Ecological research 10 (1995), S. 321-325 
    ISSN: 1440-1703
    Keywords: body temperature ; brood parasitism ; cuckoo ; evolution ; telemetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Body temperatures of 11 bird species, including cuckoos, were measured in an artificial meteorological room. Ratios of change in body temperature to that in air temperature were thereby obtained for each species. Cuckoos demonstrate a remarkably high value, indicating a particularly low ability to regulate body temperature. Viewed in this light, the cuckoo's parasitic behavior is very likely an adaptation to overcome a physiological disadvantage. This in turn might be expected to reinforce delay in evolution of temperature homeostasis.
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  • 77
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    Journal of industrial microbiology and biotechnology 17 (1996), S. 151-158 
    ISSN: 1476-5535
    Keywords: bacteriocins ; colicins ; evolution ; ecology ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In this review we focus on the ecological and evolutionary forces that determine the frequency and diversity of colicins inEscherichia coli. To begin, we describe that this killing phenotype is ubiquitous inE. coli, with as many as 50% of the isolates from a population producing colicin toxins, and that each population sampled has its own unique distribution of the more than 20 known colicin types. Next, we explore the dynamics of colicinogeny, which exhibits a typical form of frequency dependence, where the likelihood of successful colicin invasion into a population increases as the initial density of colicinogenic cells increases. We then incorporate thoughts on the evolution of chromosomal resistance to colicins and describe how resistance might influence the dynamics of colicinogen invasion and maintenance and the resulting colicin diversity. The final section deals with a genetic and phylogenetic characterization of colicins and a discussion of the evolutionary mechanisms responsible for generating colicin diversity. In this final section we provide details of the different molecular mechanisms known to play a role in generating colicin diversity, including the two most dominant forces in colincin evolution: recombination and positive, deversifying, selection.
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  • 78
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    Journal of thermal analysis and calorimetry 52 (1998), S. 537-542 
    ISSN: 1572-8943
    Keywords: fractals ; growth ; nucleation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The author presents a model based on multi-step nucleation for the heterogeneous decompositions described by nucleation and growth phenomena, taking into account the fractal nature of the nuclei.
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  • 79
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    Aquaculture international 6 (1998), S. 1-17 
    ISSN: 1573-143X
    Keywords: Atlantic salmon (Salmo salar) ; Condition factor ; feeding regimes ; growth ; proximate composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of different feeding methods on growth and slaughter traits of Atlantic salmon, Salmo salar, have been examined. Swimming activity associated with feeding was greatest amongst fish fed using automatic feeders, intermediate amongst those fed predetermined rations by hand, and least in groups of fish fed to satiation twice per day. Fish fed to satiation ate more and grew faster than did those provided with rations predicted to support high rates of growth, but the fish fed to satiation had higher feed:gain ratios than those given predetermined rations as two meals per day. The muscle tissues of the fish fed to satiation had higher lipid (%) and lower moisture (%) contents than did those of the fish fed the predetermined rations, but differences in muscle composition could be accounted for by differences in the size of the fish. Lipid was positively, and moisture negatively, correlated with fish size, whereas per cent protein and dress-out losses were little influenced by fish size and feeding regime. The fish fed to satiation were slightly heavier for a given length than those in the other groups. There was, however, no evidence that the largest fish had the highest condition factor, nor could condition factor be used to predict per cent lipid in the muscle tissue. Size variation increased in all groups of fish, and this was, in part, due to the cessation of growth of maturing individuals. Size variation was, however, most marked in the groups fed using automatic feeders, suggesting that interindividual competition for food was greatest amongst these fish.
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  • 80
    ISSN: 1573-143X
    Keywords: bighead carp (Aristichthys nobilis) ; growth ; Laguna Lake ; salinity tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Bighead carp (Aristichthys nobilis Oshima) fry of various ages (11, 18, and 35 days post-hatch) were exposed to the low salinities encountered during the annual intrusion of seawater in Laguna Lake, Philippines. Practical indices of salinity tolerance assessed the effect of a 96 h direct exposure to low salinities (0–16‰). Mean (MST) and median survival times (MST50) of fry decreased as salinity of rearing medium increased. Younger fry were less able to tolerate exposure to these salinities than their older cohorts. Median lethal salinity after 96 h (MLS) revealed higher tolerance among 35–day old fry (7.6‰) than 11 (2.3‰) and 18–day old fry (6.0‰), demonstrating that survival in saline water depends on their age at initial exposure to low salinities. Mean body weight of 18–day old fry reared in 0 and 2‰ for 3 and 4 weeks was higher than for those reared in 4 and 6‰ for the same period. Growth over these periods was inversely related with the range of salinities tested. These results demonstrate that, despite their known stenohalinity, bighead carp fry possess some degree of osmoregulatory capability, allowing them to survive and grow in lakes subjected periodically to saltwater inflow.
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  • 81
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    Aquaculture international 7 (1999), S. 287-293 
    ISSN: 1573-143X
    Keywords: bone mineralization ; calcium requirement ; feed efficiency ; growth ; semi-purified diet ; tiger puffer (Takifugu rubripes)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Juvenile tiger puffer (Takifugu rubripes) were fed semi-purified diets with graded levels (0–0.40%) of calcium (Ca) supplements for 8 weeks at a temperature of 23.0–25.5 °C. At the end of the feeding experiment, average final body weight increased with increasing dietary Ca levels. A diet without Ca supplement resulted in poor average final body weight and 0.05% dietary Ca supplement could not significantly improve the growth. However, supplemental Ca levels of 0.10–0.40% significantly increased growth. Dietary Ca supplements of 0.10% or more also improved the feed efficiency in tiger puffer. Ca and phosphorus contents of the bone were independent of dietary Ca supplements. However, 0.40% Ca in the diet decreased the zinc and manganese contents of bone. Results indicated that supplemental Ca levels of 0.10–0.20% in a semi-purified diet were required to maintain normal growth, feed utilization and bone mineralization of tiger puffer.
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    Molecular and cellular biochemistry 146 (1995), S. 63-69 
    ISSN: 1573-4919
    Keywords: rice bran oil ; growth ; plasma lipids ; fatty acids ; lipoproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effects of feeding two levels of rice bran oil (RBO) on the growth, lipid parameters, and fatty acid composition of the plasma and liver of rats (Wistar strain) were compared with those produced on animals which had been fed the same levels of peanut oil (PNO). The control animals were fed synthetic diets containing 5 and 20% peanut oil (PNO) and the experimental groups were fed similar diets, containing the same level of rice bran oil (RBO). There was no significant difference with respect to the organ weights between the control and the experimental groups. In general, groups fed 20% oil gained more weight than groups fed 5% oil. The animals which received rice bran oil in their diet had, in general, comparatively lower levels of cholesterol, triglycerides and phospholipids. On the other hand, animals receiving 20% rice bran oil in their diet, showed an increase of 20% in high density lipoproteins (HDL-C), within 18 weeks (p〈0.05), when compared to the animals fed with peanut oil. Similarly, low density lipoprotein cholesterol (LDL-C) and very low density lipoprotein cholesterol (VLDL-C) were lower in RBO-fed groups, than in the PNO-fed groups. There was, however, no significant differences in the cholesterol/phospholipid (C/P) ratio of the two groups. Analysis of plasma and of liver fatty acids indicated, in a general way, the type of fat consumed. There were no significant difference in the P/S ratio, nor any in the oleic/linoleic, oleic/stearic, palmitoleic/palmitic, oleic/palmitic, and oleic/palmitoleic ratios. Furthermore, levels of saturated (SAFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids were identical in both the groups. Thus, our results suggest that feeding a high level of rice bran oil (RBO) has no deleterious effect on the growth and blood lipid profile of rats.
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  • 83
    ISSN: 1573-4919
    Keywords: NADH oxidase ; plasma membranes ; growth ; retinol ; retinoids ; HeLa cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Several retinoids, both natural and synthetic, were evaluated for their ability to modulate NADH oxidase activity of plasma membranes of cultured HeLa cells and the growth of HeLa cells in culture. Both NADH oxidase activity and the growth of cells were inhibited by the naturally-occurring retinoids all trans-retinoic acid (tretinoin) and retinol as well as by the synthetic retinoids, trans-acitretin, 13-cis-acitretin, etretinate and arotonoid ethylester (Ro 13-6298). For all retinoids tested, inhibition of NADH oxidase activity and inhibition of growth were correlated closely. With tretinoin, etretinate and arotonoid ethylester, NADH oxidase activity and cell growth were inhibited in parallel in proportion to the logarithm of retinoid concentration over the range of concentrations 10-8 to 10-5 M. Approximately 70% inhibition of both NADH oxidase activity and growth was reached at 10 µM. With retinol, trans-acitretin and 13-cis-acitretin, inhibition of NADH oxidase activity and growth also were correlated but maximum inhibition of both was about 40% at 10 µM. The possibility is suggested that inhibition of the plasma membrane NADH oxidase activity by retinoids may be related to their mechanism of inhibition of growth of HeLa cells in culture. (Mol Cell Biochem 166: 101-109, 1997)
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  • 84
    ISSN: 1573-4919
    Keywords: heart ; postnatal development ; sarcoplasmic reticulum ; phospholamban ; calcium transport ; spontaneously hypertensive rats ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This comparative study investigates the relationship between sarcoplasmic reticulum (SR) calcium(Ca2+)-ATPase transport activity and phospholamban (PLB) phosphorylation in whole cardiac homogenates of spo`ntaneously hypertensive rats (SHR) and their parent, normotensive Wistar Kyoto (WKY) strain during early postnatal development at days 1, 3, 6, 12 and at day 40 to ascertain any difference in SR Ca2+ handling before the onset of hypertension. At day 1, the rate of homogenate oxalate-supported Ca2+ uptake was significantly higher in SHR than in WKY (0.25 ± 0.02 vs 0.12 ± 0.01 nmoles Ca2+/mg wet ventricular weight/min, respectively; p 〈 0.001). This interstrain difference disappeared with further developmental increase in SR Ca2+ transport. Western Blot analysis and a semiquantitative ELISA did not reveal any difference in the amount of immunoreactive PLB (per mg of total tissue protein) between strains at any of the ages studied. In addition, levels of phosphorylated PLB formed in vitro in the presence of radiolabelled ATP and catalytic (C) subunit of protein kinase A did not differ between SHR and WKY at days 1, 3, 6 and 12. At day 40, C subunit-catalyzed formation of 32P-PLB was reduced by 66% (p 〈 0.001) in SHR when compared to age-matched WKY In the early postnatal period between day 1 and 12 SR Ca2+-transport values were linearly related to the respective 32P-PLB levels of both SHR and WKY rats. The results indicate that cardiac SR of SHR can sequester Ca2+ at a much higher rate immediately after birth compared to WKY rats. The disappearance of this interstrain difference with further development suggests that some endogenous neuroendocrine or nutritional factor(s) from the hypertensive mother may exert an influence upon the developing heart in utero resulting in a transiently advanced maturation of the SR Ca2+ transport function in SHR pups at the time of birth.
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  • 85
    ISSN: 1573-4919
    Keywords: NADH oxidase ; protein disulfide-thiol interchange ; dipyridyl-dithio substrates ; plasma membrane ; auxin ; 2,4-dichlorophenoxyacetic acid ; growth ; plant (Glycine max)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Dipyridyl-dithio substrates were cleaved by isolated vesicles of plasma membranes prepared from etiolated hypocotyls of soybean. The cleavage was stimulated by auxins at physiological concentrations. The substrates utilized were principally 2,2′-dithiodippyrine (DTP) and 6,6′-dithiodinicotinic acid (DTNA). The DTP generated 2 moles of 2-pyridinethione whereas the 6,6′-dithiodinicotinic acid generated 2 moles of 6-nicotinylthionine. Both products absorbed at 340 nm. The auxin herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D) stimulated the activity approximately 2-fold to a maximum at about 10 μM. Concentrations of 2,4-D greater than 100 μM inhibited the activity. Indole-3-acetic acid stimulated the activity as well. The growth-inactive auxin, 2,3-dichlorophenoxyacetic acid (2,3-D), was without effect. DTNA cleavage correlated with oxidation of NADH and reduction of protein disulfide bonds reported earlier in terms of location at the external plasma membrane surface, absolute specific activity, pH dependence and auxin specificity. The dipyridyl-dithio substrates provide, for the first time, a direct measure of the disulfide-thiol interchange activity of the protein previously measured only indirectly as an auxin-dependent ability of isolated plasma membrane vesicles to restore activity to scrambled and inactive RNase.
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  • 86
    ISSN: 1573-4927
    Keywords: glycophorins ; gorilla ; evolution ; gene family ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Homologues of MN blood group antigens, encoded by members of the glycophorin A (GPA) gene family, are expressed in man, anthropoid apes, and some species of Old World monkeys. Previous studies had shown that a three-gene framework, most closely related to that in man, is present in the chimpanzee. Here we report the genomic structure, transcript map, and protein expression of the GYPA locus in gorillas. Compared to the corresponding human and chimpanzee homologues, gorilla GPA, GPB, and GPB/E genes each showed a high degree of sequence identity, with the same exon-intron organization. However, the expression of exons III, IV, or V encoding the extracellular or membrane domains of homologous glycophorins varied among the three species. Gorilla GPA and GPB/E genes were unique in that the former occurred in two allelic forms with or without the expression of exon III, whereas the latter contained one (ψ exon III) instead of two silenced exons (ψ exons III and IV). Differences from human but not chimpanzee GPA also included the presence of a hybrid M/N epitope and the absence of the sequon for N-glycosylation. Owing to the retention of a functional exon III, gorilla GPB was more similar to chimpanzee GPB than human GPB. A transspecies allele was identified in the gorilla that gave rise to the Henshaw (He)-like antigen similar to that found in man. These results provide further insight into the model for evolution of the GPA gene family, indicating that the mechanisms underlying inter- and intraspecific polymorphism of glycophorins could predate the divergence of gorillas as the consequence of gene duplication and diversification.
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  • 87
    ISSN: 1573-4919
    Keywords: annexin V ; cardiac myocyte ; growth ; hypertrophy ; heart
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Recently it was shown that annexin V is the most prominent member of the annexin family in the adult heart [1]. Amongst others, annexin V has been suggested to play a role in developmental processes. The aim of the present study was to explore whether in the heart annexin V content and localization change during maturational and hypertrophic growth, in order to obtain indications that annexin V is involved in cardiac growth processes. First, in the intact rat heart annexin V content and localization were studied during perinatal development. It was clearly demonstrated that annexin V content in total heart transiently increased in the first week after birth, from 0.79 ± 0.06 µg/mg protein at l day before birth to a peak value of 1.24 ± 0.08 µg/mg protein 6 days after birth, whereafter annexin V protein levels declined to a value of 0.70 ± 0.06 µg/mg protein at 84 days after birth (p 〈 0.05). Differences in annexin V content were also observed between myocytes isolated from neonatal and adult hearts [0.81 ± 0.09 and 0.17 ± 0.08 µg/mg protein, respectively (p 〈 0.05)]. Moreover, during cardiac maturational growth the subcellular localization of annexin V might change from a cytoplasmic to a more prominent sarcolemmal localization. Second, in vivo hypertrophy induced by aortic coarctation resulted in a marked degree of hypertrophy (22% increase in ventricular weight), but was not associated with a change in annexin V localization or content. The quantitative results obtained with intact hypertrophic rat hearts are supported by findings in neonatal ventricular myocytes, in which hypertrophy was induced by phenylephrine (10-5 M). In the latter model no changes in annexin V content could be observed either. In conclusion, the marked alterations in annexin V content during the maturational growth in the heart suggest a possible involvement of this protein in this process. In contrast, the absence of changes in annexin V content and localization in hypertrophied hearts compared to age matched control hearts suggests that annexin V does not play a crucial role in the maintenance of the hypertrophic phenotype of the cardiac muscle cell. This notion is supported by observations in phenylephrine-induced hypertrophied neonatal cardiomyocytes.
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    Aquarium sciences and conservation 2 (1998), S. 9-19 
    ISSN: 1573-1448
    Keywords: Barbus tetrazona ; nutrition ; dietary requirement-phosphorus ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of increasing dietary concentrations of phosphorus on growth, feeding efficiency and body composition were studied in the tiger barb (Barbus tetrazona) (Bleeker, 1855). Juvenile tiger barbs (initial weight 0.33 ± 0.02 g) were fed purified diets containing graded levels of KH2PO4 to provide 0.17–1.57% available dietary phosphorus for 8 weeks in freshwater glass aquaria. The weight gain, feeding efficiency and mineral composition of the whole body and bone were significantly (p〈0.05) affected by dietary phosphorus. Growth response data calculated by broken line analysis showed that the minimum dietary phosphorus level required for maximum growth was 0.52%. The carcass protein, fat and ash contents but not moisture were affected by various dietary phosphorus levels. Fish fed a low-phosphorus diet had high body crude fat.
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  • 89
    ISSN: 1573-1561
    Keywords: Eriocrania cicatricella ; Eriocrania sparrmannella ; Eriocraniidae ; Lepidoptera ; sex pheromone ; EAG ; GC-EAD ; mass spectrometry ; synthesis ; evolution ; (Z)-4-hepten-2-one ; (2R)-heptan-2-ol ; (2R)-(Z)-4-hepten-2-ol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Extracts from different body parts of adult femaleEriocrania cicatricella (Zett.) were tested for electrophysiological activity on conspecific male antennae. Extracts from the Vth abdominal segment, containing a pair of exocrine glands, elicited the largest electroantennographic response when compared to extracts of other body parts. Female extracts were analyzed by gas chromatography with simultaneous flame ionization and electroantennographic detection (EAD). The EAD active peaks were identified as (Z)-4-hepten-2-one, (2R)-heptane-2-ol, and (2R)-(Z)-4-hepten-2-ol by coinjection on a gas chromatography and by comparison of mass spectra with those of synthetic standards. In field tests, a blend of these three pheromone components was highly attractive to conspecific males, and a subtractive assay confirmed that the unsaturated alcohol is the major pheromone component, whereas no definite behavioral activity could be assigned to the ketone or the saturated alcohol. A bait containing the two alcohols withS-configuration was attractive to maleE. sparrmannella (Bosc), whereas no males ofE. cicatricella were found in these traps. The sex pheromone compounds inE. cicatricella are chemically similar to pheromones reported in Trichoptera and they are produced in homologous glands.
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  • 90
    ISSN: 1573-1561
    Keywords: Sex pheromone ; Idaea aversata ; Idaea straminata ; Idaea biselata ; (Z,Z)-9,11-tetradecadienyl acetate ; (Z,Z)-7,9-dodecadienyl acetate ; (Z,E)-7,9-dodecadienyl acetate ; Lepidoptera ; Geometridae ; electroantennography ; single cell recording ; biosynthesis ; phylogeny ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Pheromone compounds so far identified from most geometrid moths consist of all-Z diene, triene, or tetraene hydrocarbons with chain lengths of C17 to C21, and their monoepoxide derivatives biosynthesized from linoleic and linolenic acids. The present study reports the occurrence of olefinic acetates as sex pheromones in three species of Geometridae. (Z,Z)-9,11-tetradecadienyl acetate and (Z,Z)-7,9-dodecadienyl acetate found in female gland extracts ofIdaea aversata elicited significant responses from conspecific male antennae in gas chromatography with electroantennographic detection (GCEAD). In extracts ofI. straminata, (Z,Z)-7,9-dodecadienyl acetate, (E,Z)-7,9-dodecadienyl acetate, and (Z,Z)-7,9-dodecadienyl acetate were found, and the synthetic compounds elicited strong responses from conspecific male antennae. In the third species,I. biselata, only (Z,Z)-7,9-dodecadienyl acetate was found in the female extracts, and this compound elicited a strong EAD response from the conspecific male antenna. The identities of the pheromone components inI. aversata andI. straminata were further confirmed according to their characteristic ions after GC-MS analyses. Single sensillum recordings fromI. aversata showed two types of pheromone-detecting sensilla present on the male antenna. One type contained two receptor neurons, one of which was specifically tuned to (Z,Z)-9,11-tetradecadienyl acetate, the other to (Z,E)-9,11-tetradecadienyl acetate. A second type contained one neuron responding to (Z,Z)-7,9-dodecadienyl acetate. The two types were clearly different also with respect to external morphology, the former being considerably longer and having a larger base diameter. Also inI. straminata two physiological types of sensilla could be distinguished. One type contained two neurons, one of which responded to (Z,Z)-7,9-dodecadienyl acetate, the other to (Z,E)-9,11-tetradecadienyl acetate. The second type contained one neuron, responding to (Z,Z)-7,9-dodecadienyl acetate. No correlation between external morphology and physiological response of the investigated sensilla was observed inI. straminata. In field tests, a two-component blend containing (Z,Z)-9,11-tetradecadienyl acetate and (Z,Z)-7,9-dodecadienyl acetate in a ratio of 10:1 was attractive to males ofI. aversata. This two-component blend was also attractive to males ofI. straminata, but in a ratio of 1:1. High numbers of maleI. biselata were caught in traps baited with (Z,Z)-7,9-dodecadienyl acetate alone. The incorporation of deuterium labels into pheromone components after topical application of deuterium-labeled palmitic acid confirmed that the pheromone components ofI. aversata could be synthesized from this precursor, as has been previously observed for acetate pheromone components of many other moth species. Our results suggest that an evolutionary reversal back to the production of palmitic acid-derived pheromone components has occurred within the geometrid subfamily Sterrhinae.
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  • 91
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    Journal of chemical ecology 23 (1997), S. 1527-1547 
    ISSN: 1573-1561
    Keywords: Secondary metabolites ; chemical defense ; evolution ; ascidians ; sponges
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We used three chemical fractions (spanning a wide range of polarities) from the extracts of four marine invertebrates, the spongesCrambe crambe andHemimycale columella and the ascidiansCystodytes dellechiajei andPolysyncraton lacazei, to test inhibition of cell division, photosynthesis, and settlement. We used assay organisms from the same habitat, seeking to determine whether a species may display diverse, ecologically relevant bioac-tivities and, if so, whether the same types of compound may be responsible for such activities. Cell division was strongly inhibited by the spongeC. crambe. A dichloromethane fraction fromC. crambe prevented development of sea urchinParacentrotus lividus eggs at a concentration of 10 μg/ml, as did the butanolic fraction, but at higher concentrations (50 and 100 μg/ml). At 50 μg/ml, the aqueous fraction ofC. crambe allowed cell division but prevented eggs from developing beyond the gastrula stage. Similar results were recorded with the dichloromethane fraction ofP. lacazei and from the aqueous fraction ofH. columella. Photosynthesis was unaffected by any of the species at 50 μg/ml. Larval settlement was inhibited by one or another fraction from the four species surveyed at a concentration of 50 μg/ml, althoughC. crambe exhibited the greatest amount of activity. We therefore found that various fractions displayed the same type of bioactivity, while compounds from the same fraction were responsible for multiple activities, suggesting that secondary metabolites are multiple-purpose tools in nature, which is relevant to our understanding of species ecology and evolution. Moreover, results showed that the assessment of the role of chemical compounds is significantly influenced by the assay organism, fractionation procedure, concentration, and duration of experiments. All these factors should be carefully considered when testing ecological hypotheses of the roles of chemically-mediated bioactivities.
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  • 92
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    Journal of chemical ecology 25 (1999), S. 31-49 
    ISSN: 1573-1561
    Keywords: Chemical ecology ; evolution ; variation ; population dynamics ; community ; species interactions ; infochemical ; semiochemical ; parasitoid ; foraging behavior ; learning ; phenotypic plasticity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The marriage of chemistry with ecology has been a productive one, providing a wealth of examples of how chemicals play important roles in the loves and lives of living organisms. At first the marriage may have been a simple and monogamous one with the major scientific aim of making proximate analyses of chemically mediated, individual level interactions. But times have changed and chemical ecology is broadening, embracing different approaches and disciplines. There is, for example, increasing appreciation of variability in the systems under study and an increase in evolutionary thinking. Another promising development is greater recognition of the potential importance of chemically mediated interactions for population dynamics and for structuring communities and species coexistence. The latter is an utterly underexplored area in chemical ecology. The field of chemical ecology of insect parasitoids shows some of these promising developments. Responses of parasitoids to infochemicals are increasingly studied with an integrated approach of mechanism and function. This integration of “how” and “why” questions significantly enhances the evolutionary and ecological understanding of stimulus–response patterns. The future challenge in chemical ecology is to demonstrate how chemically mediated interactions steer ecological and evolutionary processes at all levels of ecological organization. To reach this goal there is a need for interdisciplinary collaboration among chemists and ecologists working at different levels of organization and with different approaches, with other disciplines as partners.
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  • 93
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    Journal of chemical ecology 25 (1999), S. 1455-1479 
    ISSN: 1573-1561
    Keywords: Turnera ulmifolia ; Euptoieta hegesia ; cyanogenesis ; Anolis sagrei ; tritrophic interactions ; host range ; sequestration ; growth ; development ; oviposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We examine the effects of the cyanogenesis polymorphism in Turnera ulmifolia on larvae, pupae, and adults of Euptoieta hegesia, the most damaging herbivore of T. ulmifolia in terms of tissue loss per unit time. We provide evidence that female E. hegesia do not show preference for host plants on the basis of their cyanogenesis level but do prefer T. ulmifolia over equally cyanogenic, closely related secondary host-plant species (Passiflora sp.). Similarly, cyanogenesis in T. ulmifolia has little effect on the food preference, growth, or development of the larvae. The potential host range of E. hegesia is limited, even within the genus Turnera, but this does not appear to be due to host-plant cyanogenesis. Pupae suffer very high mortality levels in the wild that are not associated with host-plant cyanogenesis, although our studies indicate that larvae are capable of sequestering cyanogenic glycosides from their host plants and possibly of synthesizing these or similar compounds. We provide evidence that the presence of sequestered cyanogenic compounds in the larvae protects them from terrestrial-based predators such as Anolis lizards
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  • 94
    ISSN: 1573-2932
    Keywords: Fish populations ; perch ; roach ; growth ; reproduction ; water chemistry ; acidification ; recover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Acid-induced fish damage in small lakes in southern Finland was studied in a fish status survey of eighty lakes from 1985–1987. Later, twenty of these lakes were selected for further monitoring. A sampling of these lakes from 1988–1989 showed that the decrease in some perch (Perca fluviatilis L.) and roach (Rutilus rutilus L.) populations still continued. The results from the same lakes in 1992 showed that successful reproduction had taken place with many of the perch populations that had been close to extinction in 1985. In contrast, no signs of recovery in the roach populations were detected. The explanation for the appearance of new cohorts of perch could have been the decrease in acid deposition but the exceptional hydrological conditions of winters in the early 1990s may also have affected them. The different responses of the perch and roach populations were interpreted as a consequence of the different sensitivity of these two species to acidification. Even a slight improvement in the water quality has resulted in the appearance of strong new year-classes of perch, but not of roach. Therefore, more improvement in water quality is needed until a sensitive species like roach can reproduce again.
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  • 95
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    Water, air & soil pollution 85 (1995), S. 1461-1466 
    ISSN: 1573-2932
    Keywords: ozone ; wheat ; Triticum aestivum ; growth ; senescence ; biomass partitioning ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In closed-chamber fumigation experiments dry matter partitioning and chlorophyll fluorescence of wheat were studied, analysing the effects of ozone during different stages of plant development. Ozone causes enhanced leaf senescence, leading to a loss of green leaf area and, consequently to a decreased supply of assimilates, affecting (in increasing order of severeness) stem, ear and grain productivity because of reduced storage pools for translocation. Leaves of plants before shooting stage were most sensitive but the lack of green leaf area after ear emergence had the most pronounced effects on grain yield. Measurements of photochemical capacity showed that evidence for negative ozone effects could be found in changes of chlorophyll fluorescence parameters in leaf sections not yet showing visible ozone injury. Negative effects on photosynthesis were more distinct with increasing accumulated ozone dose, with increasing age of leaf tissue and with increasing ozone sensitivity of the cultivar. The changes in chlorophyll fluorescence are most likely to be explained by a decreased pool size of plastoquinones caused by ozone.
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  • 96
    ISSN: 1573-3017
    Keywords: Tubifex tubifex ; sediment bioassay ; hormesis ; growth ; reproduction ; egestion rates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Toxicity assessment based on sediment chronic bioassays with the aquatic worm Tubifex tubifex was performed at ten contaminated sites in the industrial area of Bilbao (Northern Spain). One control and three reference sites were also included. Tubifex bioassay measures both survival and reproduction impairment. These endpoints have been contrasted and discussed in relation to somatic growth and both individual and total biomass of cocoons. Survival was only affected at one site which was heavily contaminated by organic compounds, mainly PAHs. A group of four severely ecotoxic sediments was characterised by a drastic reduction in number and size of cocoons, and adult somatic growth. In other group of sediments, some significant increases were found for these variables. It is suggested that these increases represent an effect of hormesis. An index of reproductive effort was used to integrate the relationship between somatic growth and reproduction. Values of reproductive effort at the test sediments were lower than those at the control sediment, suggesting a conservative strategy of oligochaete worms which consisted in an investment into somatic line (growth) at the expenses of offspring. Rates of food consumption which were estimated from egestion rates, were low at the contaminated sites. This fact could be related to the low production levels found at these sites and may reflect avoidance feeding behaviour of the oligochaete worms within the sediments. At some reference sites, high production could have resulted from high nutritional quality of sediments, or to an hormetic effect due to low concentration of some chemicals. Results are discussed in relation to toxicity data from sediment three-brood bioassay with Daphnia magna Straus performed separately on the same sediments.
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  • 97
    ISSN: 1573-4919
    Keywords: guanine nucleotide-binding proteins ; evolution ; phylogeny ; structure-function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract ADP-ribosylation factors (ARFs) are ∼20-kDa guanine nucleotide-binding proteins that are allosteric activators of the NAD:arginine ADP-ribosyltransferase activity of cholera toxin and appear to play a role in intracellular vesicular trafficking. Although the physiological roles of these proteins have not been defined, it has been presumed that each has a specific intracellular function. To obtain genetic evidence that each ARF is under evolutionary pressure to maintain its structure, and presumably function, rat ARF cDNA clones were isolated and their nucleotide and deduced amino acid sequences were compared to those of other mammalian ARFs. Deduced amino acid sequences for rat ARFs 1, 2, 3, 5 and 6 were identical to those of the known cognate human and bovine ARFs; rat ARF4 was 96% identical to human ARF4. Nucleotide sequences of both the untranslated as well as the coding regions were highly conserved. These results indicate that the ARF proteins are, as a family, extraordinarily well conserved across mammalian species. The unusually high degree of conservation of the untranslated regions is consistent with these regions having important regulatory roles and that individual ARFs contain structurally unique elements required for specific functions.
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  • 98
    ISSN: 1573-4943
    Keywords: Klebsiella aerogenes ; ribitol dehydrogenase ; evolution ; mutant structures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A mutant ribitol dehydrogenase (RDH-F) was purified from Klebsiella aerogenes strain F which evolved from the wild-type strain A under selective pressure to improve growth on xylitol, a poor substrate used as sole carbon source. The ratio of activities on xylitol (500 mM) and ribitol (50 mM) was 0.154 for RDH-F compared to 0.033 for the wild-type (RDH-A) enzyme. The complete amino acid sequence of RDH-F showed the mutations. Q60 for E60 and V215 for L215 in the single polypeptide chain of 249 amino acid residues. Structural modeling based on homologies with two other microbial dehydrogenases suggests that E60 → Q60 is a neutral mutation, since it lies in a region far from the catalytic site and should not cause structural perturbations. In contrast, L215 → V215 lies in variable region II and would shift a loop that interacts with the NADH cofactor. Another improved ribitol dehydrogenase, RDH-D, contains an A196 → P196 mutation that would disrupt a surface α-helix in region II. Hence conformational changes in this region appear to be responsible for the improved xylitol specificity.
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  • 99
    ISSN: 1573-4927
    Keywords: Akodon ; Cricetidae rodents ; genetic diversity ; biochemical polymorphism ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The present study involved an electrophoretic survey of 22 protein loci in 269 individuals belonging to three species of the genusAkodon, A. aff.cursor (2n=16),A. cursor (2n=14/15), andA. montensis (2n=24/25/26), collected in Eastern Brazil. The joint results of gene diversity, genetic distances, phenetic analyses, and phylogenetic trees suggested thatA. aff.cursor has recently separated fromA. cursor and that the three species have experienced a recent chromosomal divergence followed by low allozyme differentiation. These data are in agreement with their classification as sibling species.
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  • 100
    ISSN: 1573-4935
    Keywords: Chemotaxis ; evolution ; oligopeptides ; Tetrahymena ; Dunaliella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Chemotactic properties of amino acids (L-alanine, glycine and L-lysine) and their oligopeptides (10−6M) and binding sites to these ligands were investigated in two unicellular models, the heterotrophicTetrahymena pyriformis and the auxotrophicDunaliella salina. Chemotaxis ofDunaliella induced by simple amino acids and their derivatives demonstrated that binding sites (receptors) for food molecules are not only present in the membrane but are also able to induce their basic physiological response. InTetrahymena, substances with special molecular structure and properties (polar, hydrophilic character of the signal peptide chain)-5-L-Lys, 5-Glywere required for chemoattraction, other peptides tested, lacking the required structure, were repellent. Divergences in chemotaxis and binding assays of both species suggest that trends of functional and binding parameters do not run parallel at this level of evolution.
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