ALBERT

Description: Climate change has significant implications for biodiversity and ecosystems. With slow progress towards reducing greenhouse gas emissions, climate engineering (or ‘geoengineering’) is receiving increasing attention for its potential to limit anthropogenic climate change and its damaging effects. Proposed techniques, such as ocean fertilization for carbon dioxide removal or stratospheric sulfate injections to reduce incoming solar radiation, would significantly alter atmospheric, terrestrial and marine environments, yet potential side-effects of their implementation for ecosystems and biodiversity have received little attention. A literature review was carried out to identify details of the potential ecological effects of climate engineering techniques. A group of biodiversity and environmental change researchers then employed a modified Delphi expert consultation technique to evaluate this evidence and prioritize the effects based on the relative importance of, and scientific understanding about, their biodiversity and ecosystem consequences. The key issues and knowledge gaps are used to shape a discussion of the biodiversity and ecosystem implications of climate engineering, including novel climatic conditions, alterations to marine systems and substantial terrestrial habitat change. This review highlights several current research priorities in which the climate engineering context is crucial to consider, as well as identifying some novel topics for ecological investigation.

Description: The surface chemistry of aquatic organisms determines their biotic interactions. Metabolites in the spatially limited laminar boundary layer mediate processes, such as antifouling, allelopathy and chemical defense against herbivores. However, very few methods are available for the investigation of such surface metabolites. An approach is described in which surfaces are extracted by means of C18 solid phase material. By powdering wet algal surfaces with this material, organic compounds are adsorbed and can be easily recovered for subsequent liquid chromatography/mass spectrometry (LC/MS) and gas chromatography/mass spectrometry (GC/MS) investigations. The method is robust, picks up metabolites of a broad polarity range and is easy to handle. It is more universal compared to established solvent dipping protocols and it does not cause damage to the test organisms. A protocol is introduced for the macroalgae Fucus vesiculosus, Caulerpa taxifolia and Gracilaria vermiculophylla, but it can be easily transferred to other aquatic organisms.

Description: Different studies on the position of the non-indigenous species Neogobius melanostomus within the coastal food web of the Pomeranian Bay (western Baltic) were performed, resulting in a quantitative and qualitative species list of prey organisms found in the stomachs of the invader and an estimation concerning the importance of round goby as prey for different resident predators. It seems that the colonization process is not fully completed yet, but the results reveal that the species is already established in the food web 16 years after the first observation within the study area. The results show that N. melanostomus feed upon a wide range of different resident organisms. While a direct predation effect on native fish species appears rather unlikely, indirect effects such as competition cannot yet be excluded. In addition, our results reveal an ontogenetic diet shift and that the round goby itself already serves as an important prey for piscivorous fish and seabirds. Finally, we formulate different hypotheses based on our results which will require further research.

Description: International Ocean Discovery Program (IODP) Expedition 352 recovered a high-fidelity record of volcanism related to subduction initiation in the Bonin fore-arc. Two sites (U1440 and U1441) located in deep water nearer to the trench recovered basalts and related rocks; two sites (U1439 and U1442) located in shallower water further from the trench recovered boninites and related rocks. Drilling in both areas ended in dolerites inferred to be sheeted intrusive rocks. The basalts apparently erupted immediately after subduction initiation and have compositions similar to those of the most depleted basalts generated by rapid sea-floor spreading at mid-ocean ridges, with little or no slab input. Subsequent melting to generate boninites involved more depleted mantle and hotter and deeper subducted components as subduction progressed and volcanism migrated away from the trench. This volcanic sequence is akin to that recorded by many ophiolites, supporting a direct link between subduction initiation, fore-arc spreading, and ophiolite genesis.

Description: The production of shiga toxin (Stx) is a critical step in the establishment and progress of enterohemorrhagic Escherichia coli (EHEC) infections. The possible release of Stx from dead and dying bacteria, and the risk of resistance development have restricted the usage of antibiotics against EHEC. The chlorinated quaternary ammonium compound, strepthonium A, was isolated from the culture of Streptomyces sp. SBT345 that was cultivated from the Mediterranean sponge Agelas oroides. The structure was elucidated and confirmed by spectroscopic analyses including 1D and 2D NMR, ESI-HRMS, as well as ESI-HRMS2. Strepthonium A follows Lipinski's rule of five with respect to its molecular weight, CLogP values and the number of hydrogen acceptors and donors. Verotoxin ELISA assay demonstrated that Strepthonium A reduced the Stx production in EHEC strain EDL933 at 80 μM concentration without growth inhibition. This study demonstrates the potential of strepthonium A in restraining the production of Stx in EHEC infections.

Description: Land-use dynamic is a major challenge for town and country planners especially in developing countries such as Iran. Iran has been under rapid urban expansion and population growth for past three decades which led to lack of resources, environmental deterioration and haphazard landscape development. In this paper, an attempt has been made to map the urbanization dynamics of Tehran in 40 years based on remote sensing imagery and by means of artificial neural networks. The presented scheme could be taken into consideration when planning initiatives aimed at surveying, monitoring, managing and sustainable development of the territory. Moreover, it can serve the experts in the fields of geography, urban studies and planning as a background for number of geographical analyses.

Description: Current antifouling (AF) technologies are based on the continuous release of biocides into the water, and consequently discharge into the environment. Major efforts to develop more environmentally friendly coatings require efficient testing in laboratory assays, followed by field studies. Barnacles are important fouling organisms worldwide, increasing hydrodynamic drag on ships and damaging coatings on underwater surfaces, and thus are extensively used as models in AF research, mostly in static, laboratory-based systems. Reliable flow-through test assays for the screening of biocide-containing AF paints, however, are rare. Herein, a flow-through bioassay was developed to screen for diverse low-release biocide paints, and to evaluate their effects on pre- and post-settlement traits in barnacles. The assay distinguishes between the effects from direct surface contact and bulk-water effects, which are crucial when developing low-emission AF coatings. This flow-through bioassay adds a new tool for rapid laboratory-based first-stage screening of candidate compounds and novel AF formulations.

Description: One new diterpene, trichocitrin (1), and four known secondary metabolites, nafuredin (2), 5-hydroxy-2,3-dimethyl-7-methoxychromone (3), 24-methylenecycloartanol (4) and citrostadienol (5), were isolated from the culture of marine brown alga-endophytic Trichoderma citrinoviride cf-27. Trichocitrin (1) represents the first Trichoderma-derived and furan-bearing fusicoccane diterpene, and its structure and relative configuration were identified by analysis of 1D/2D NMR and mass spectroscopic data. Compounds 1 and 2 exhibited 8.0- and 9.5-mm inhibition zones, respectively, against Escherichia coli at 20 μg/disc and 54.1 and 36.7% growth inhibition, respectively, of Prorocentrum donghaiense at 80 μg/mL.

Description: New biostratigraphical, geochemical, and magnetic evidence is synthesized with IODP Expedition 352 shipboard results to understand the sedimentary and tectono-magmatic development of the Izu–Bonin outer forearc region. The oceanic basement of the Izu–Bonin forearc was created by supra-subduction zone seafloor spreading during early Eocene (c. 50–51 Ma). Seafloor spreading created an irregular seafloor topography on which talus locally accumulated. Oxide-rich sediments accumulated above the igneous basement by mixing of hydrothermal and pelagic sediment. Basaltic volcanism was followed by a hiatus of up to 15 million years as a result of topographic isolation or sediment bypassing. Variably tuffaceous deep-sea sediments were deposited during Oligocene to early Miocene and from mid-Miocene to Pleistocene. The sediments ponded into extensional fault-controlled basins, whereas condensed sediments accumulated on a local basement high. Oligocene nannofossil ooze accumulated together with felsic tuff that was mainly derived from the nearby Izu–Bonin arc. Accumulation of radiolarian-bearing mud, silty clay, and hydrogenous metal oxides beneath the carbonate compensation depth (CCD) characterized the early Miocene, followed by middle Miocene–Pleistocene increased carbonate preservation, deepened CCD and tephra input from both the oceanic Izu–Bonin arc and the continental margin Honshu arc. The Izu–Bonin forearc basement formed in a near-equatorial setting, with late Mesozoic arc remnants to the west. Subduction-initiation magmatism is likely to have taken place near a pre-existing continent–oceanic crust boundary. The Izu–Bonin arc migrated northward and clockwise to collide with Honshu by early Miocene, strongly influencing regional sedimentation.

Description: In this study, a series of drained triaxial tests were performed in order to examine the effect of cementation on the shear behavior of granular soil. It was observed that the brittle nature and dilative tendency of granular soil is dominant under a low confining stress level, while high confining stress results in a contractive behavior despite the strong cementation bond. Based on experimental results, an idealized concept is suggested to define the shear strength of cemented sand in three distinctive zones: the cementation control zone with a constant cohesion intercept at a low confining stress level, the transition zone in which the cohesion intercept is gradually reduced after a breaking point, and the stress control zone with almost zero cohesion intercept due to breakage of cementation bonds at a high confining stress level. It was shown that the sitting pressures during cementation have little effect on the strength parameters of cemented sand, while the increase of gypsum content and relative density, and the decrease of particle size, result in an increase of the cohesion intercept and the breaking point. In addition, the prediction equations for the shear strength and cohesion intercept in the cementation control zone and the transition zone are suggested from the analytical and experimental interpretation.

Description: Antarctica is a still unexplored area in many respects, among which biological diversity and, even more so, chemical diversity are important challenges. Over recent years, our research group has been studying marine benthic ecosystems in different Antarctic areas. These studies yielded several interesting discoveries, including species new to science, as well as new natural products never seen before. Overall, we have been trying to unravel the complex network of interactions that exist between marine benthic organisms in these cold and hardly accessible ecosystems. We present here an overview of our recent results regarding the biological and the chemical diversity of these organisms. In fact, these ecosystems are as complex as many other marine environments on the planet, and indeed, many questions remain yet to be answered.

Description: IODP Expedition 350 was the first to be drilled in the rear part of the Izu-Bonin, although several sites had been drilled in the arc axis to fore-arc region; the scientific objective was to understand the evolution of the Izu rear arc, by drilling a deep-water volcaniclastic section with a long temporal record (Site U1437). The Izu rear arc is dominated by a series of basaltic to dacitic seamount chains up to ~100-km long roughly perpendicular to the arc front. Dredge samples from these are geochemically distinct from arc front rocks, and drilling was undertaken to understand this arc asymmetry. Site U1437 lies in an ~20-km-wide basin between two rear arc seamount chains, ~90-km west of the arc front, and was drilled to 1804 m below the sea floor (mbsf) with excellent recovery. We expected to drill a volcaniclastic apron, but the section is much more mud-rich than expected (~60%), and the remaining fraction of the section is much finer-grained than predicted from its position within the Izu arc, composed half of ashes/tuffs, and half of lapilli tuffs of fine grain size (clasts 〈3 cm). Volcanic blocks (〉6.4 cm) are only sparsely scattered through the lowermost 25% of the section, and only one igneous unit was encountered, a rhyolite peperite intrusion at ~1390 mbsf. The lowest biostratigaphic datum is at 867 mbsf (~6.5 Ma), the lowest palaeomagnetic datum is at ~1300 mbsf (~9 Ma), and the rhyolite peperite at ~1390 mbsf has yielded a U–Pb zircon concordia intercept age of (13.6 + 1.6/−1.7) Ma. Both arc front and rear arc sources contributed to the fine-grained (distal) tephras of the upper 1320 m, but the coarse-grained (proximal) volcaniclastics in the lowest 25% of the section are geochemically similar to the arc front, suggesting arc asymmetry is not recorded in rocks older than ~13 Ma.

Description: In this opinion paper, we, a group of scientists from environmental-, geo-, ocean- and information science, argue visual data exploration should become a common analytics approach in Earth system science due to its potential for analysis and interpretation of large and complex spatio-temporal data. We discuss the challenges that appear such as synthesis of heterogeneous data from various sources, reducing the amount of information and facilitating multidisciplinary, collaborative research. We argue that to fully exploit the potential of visual data exploration, several bottlenecks and challenges have to be addressed: providing an efficient data management and an integrated modular workflow, developing and applying suitable visual exploration concepts and methods with the help of effective and tailored tools as well as generating and raising the awareness of visual data exploration and education. We are convinced visual data exploration is worth the effort since it significantly facilitates insight into environmental data and derivation of knowledge from it.

Description: In this study, Lade's double-surface work-hardening constitutive model was adopted which uses the elasto-plasticity model as a basic conceptual framework. The model can analyze work hardening and work softening of nonlinear stress-strain behavior, and is regarded as superior to other elasto-plasticity constitutive models in terms of estimation. In the double-surface work-hardening constitutive model, 14 soil parameters are needed to estimate soil behaviors. To determine them, laboratory tests—isotropical consolidation test and conventional compression test—were conducted. Determining of soil parameters is highly complicated and time-consuming; randomness cannot be ruled out in determining parameters that are sensitive to stress-strain estimation, and error may occur. For this reason, a linear and nonlinear regression analysis was used to determine soil parameters. In estimation of undrained behavior, the estimated stress-strain behavior based on the two constitutive models largely overlapped with the test results. However, in estimating drained behavior, the outcome of the two models and the test results were mostly the same, but between the two models, the double-surface work-hardening constitutive model had a sharper slope in initial stress state, and a smaller maximum deviatoric stress.

Description: The distribution of five dominant calanoid copepods was related to different water masses in the Angola-Benguela Front system. Five water bodies were identified by principal component analysis, on the basis of abiotic parameter such as temperature, salinity, dissolved oxygen, phosphate, silicate, nitrate and nitrite. These parameters were reduced to single factors and arranged along two principal component axes. The copepod species incuded females and copepodites C5 of Calanoides carinatus and females of Metridia lucens, Centropages brachiatus, Nannocalanus minor and Aetideopsis carinata. The water bodies identified in the frontal system were related to currents, upwelling processes, an oxygen minimum layer and biological modification. The different copepod species, as well as the two ontogenetic stages of C. carinatus, showed clear preference for specific water bodies, and their behavioural and physiological adaptations to the environment are discussed.

Description: Force chain buckling, leading to unjamming and shear banding, is examined quantitatively via a discrete element analysis of a two-dimensional, densely-packed, cohesionless granular assembly subject to quasistatic, boundary-driven biaxial compression. A range of properties associated with the confined buckling of force chains has been established, including: degree of buckling, buckling modes, spatial and strain evolution distributions, and relative contributions to non-affine deformation, dilatation and decrease in macroscopic shear strength and potential energy. Consecutive cycles of unjamming–jamming events, akin to slip–stick events arising in other granular systems, characterize the strain-softening regime and the shear band evolution. Peaks in the dissipation rate, kinetic energy and local non-affine strain are strongly correlated: the largest peaks coincide with each unjamming event that is evident in the concurrent drops in the macroscopic shear stress and potential energy. Unjamming nucleates from the buckling of a few force chains within a small region inside the band. A specific mode of force chain buckling, prevalent in and confined to the shear band, leads to above-average levels of local non-affine strain and release of potential energy during unjamming. Ongoing studies of this and other buckling modes from a structural stability standpoint serve as the basis for the formulation of internal variables and associated evolution laws, central to the development of thermomicromechanical constitutive theory for granular materials.

Description: The aim of this study was to test the effects of temperature on phenolic content of the brown seaweed Cystoseira amentacea. Phenolic compounds are secondary metabolites involved in different protection mechanisms as, for example, against grazers, epiphytes and UV radiation. Seasonal variations of phenolic content in C. amentacea were analysed and laboratory experiments, in which C. amentacea was exposed to an increase of temperature (25°C and 30°C), were performed. Total phenolic content (TPC) was determined colorimetrically with the Folin–Ciocalteu reagent. In C. amentacea, a seasonal pattern in TPC was observed, with a maximum value in winter-spring. C. amentacea responded significantly to the temperature treatments with a consistent decrease in TPC after 48 h.

Description: Fluorescent-amplified fragment length polymorphism (FAFLP) fingerprinting assay was used to compare the genetic diversity within and between tadpole shrimps (Notostraca) populations of Lepidurus apus (n=7) and Triops cancriformis (n=2) from rain pools in Israel. Each ephemeral water body has revealed a unique fingerprint pattern with an entailed genetic drift between nearby ponds. High similarity of genotypic diversity within each geographic area led to three clusters of water bodies, north, south and center of Israel. FAFLP assays on several newly hatched individuals of T. cancriformis revealed high identity amongst kin, as compared to L. apus where newly hatched from the same maternal source showed high diversity. Results indicate that T. cancriformis populations from Israel are probably parthenogenetic as indicated by clonal structures. The higher genetic variability in the L. apus populations and in laboratory-hatched specimens indicates the existence of sexual reproduction.

Description: Gammarids from the Caspian complex have invaded many European waters along the rivers and canals of the inland migration corridors. The species examined in this work are well known as invaders of European freshwater environments, so the colonization of brackish habitats is a phenomenon inviting more detailed investigation. The aim of this study was to determine the condition of the Ponto-Caspian gammarids Pontogammarus robustoides (G.O. Sars, 1894), Obesogammarus crassus (G.O. Sars, 1894), Dikerogammarus haemobaphes (Eichwald, 1841) and Dikerogammarus villosus (Sowinsky, 1894) as expressed by the relationship between total length and the wet weight of specimens in the brackish waters of the Gulf of Gdansk (Poland). The relationships can be regarded as responses to a newly expanded habitat after they overcome the salinity barrier of the southern Baltic Sea coastal waters. All these Ponto-Caspian gammarids demonstrated an increase in weight with increasing total length: P. robustoides (b = 2.852), O. crassus (b = 3.3477), D. haemobaphes (b = 3.7855) and D. villosus (b = 2.6917). The results are an indicator of the relatively good condition of the organisms and indicate that the brackish environment of the Gulf of Gdansk affords them excellent possibilities for growth.

Description: A bioassay-guided approach was used to identify defense compounds that are present on the surface of Zostera marina and which inhibit settlement of microfoulers at natural concentrations. Moderately polar eelgrass surface extracts inhibited the settlement of seven marine bacteria and one yeast that originated from non-living substrata. In contrast, five other bacterial strains that had been directly isolated from eelgrass surfaces were all insensitive, which suggested a selective effect of surface metabolites on the microbial communities present on eelgrass. Bioassay-guided isolation of active compounds from the extracts in combination with UPLC-MS and 1H-NMR spectroscopy resulted in the identification of rosmarinic acid, luteolin-7-sulfate and diosmetin-7-sulfate or its isomer chrysoeriol-7-sulfate. All three compounds are nontoxic repellents, as they did not inhibit bacterial growth, but prevented bacterial settlement in a dose-dependent manner. Between 15.6 and 106.8 μg ml−1 of rosmarinic acid were present on the eelgrass surface, enough for half maximal settlement inhibition of bacteria.

Description: A new 30-arc second resolution global topography/bathymetry grid (SRTM30_PLUS) has been developed from a wide variety of data sources. Land and ice topography comes from the SRTM30 and ICESat topography, respectively. Ocean bathymetry is based on a new satellite-gravity model where the gravity-to-topography ratio is calibrated using 298 million edited soundings. The main contribution of this study is the compilation and editing of the raw soundings, which come from NOAA, individual scientists, SIO, NGA, JAMSTEC, IFREMER, GEBCO, and NAVOCEANO. The gridded bathymetry is available for ftp download in the same format as the 33 tiles of SRTM30 topography. There are 33 matching tiles of source identification number to convey the provenance of every grid cell. The raw sounding data, converted to a simple common format, are also available for ftp download.

Description: Cirripedes are one of the major groups of fouling organism in the marine environment. The cyprid can, before a permanent attachment, actively explore and walk on the substratum using its antennules in a bipedal fashion without leaving the surface. Studying the structure of the cyprid antennule is therefore important for understanding the events that culminate in biofouling by barnacles. There are at present no complete, standardised accounts of the structure of the cyprid antennules in thoracican barnacles, and moreover, the existing accounts vary in their use of terminology. This article describes the cyprid antennule of the barnacle Megabalanus rosa. This barnacle species is common in E Asia, and the cyprids have previously been used in several biofouling studies. All externally visible setae on the antennules have been mapped; these comprise both chemosensors with a terminal pore, a putative aesthetasc-like seta and mechano-sensory setae. More setae were found on the attachment disc than in previous scanning electron microscope-based studies, but not all structures that can be seen with transmission electron microscopy were visible. The disc itself seems to have a variable surface area, which could assist in exploring rough surfaces. The various lengths of the antennular setae, coupled with the disposition of the segments, enable the cyprid to cover a wide swath of substratum during exploratory walking. A new terminology is proposed for cyprid antennular setae, which will form a basis for future comparative and functional studies of cirripede settlement.

Description: For the environmental monitoring of coral, mucus appears to be an appropriate biological matrix due to its array of functions in coral biology and the non-intrusive manner in which it can be collected. The aim of the present study was to evaluate the feasibility of using mucus of the stony coral Lophelia pertusa (L. pertusa) as an analytical matrix for discovery of biomarkers used for environmental monitoring. More specifically, to assess whether a mass-spectrometry-based proteomic approach can be applied to characterize the protein composition of coral mucus and changes related to petroleum discharges at the seafloor. Surface-enhanced laser desorption/ionization–time of flight mass spectrometry (SELDI-TOF MS) screening analyses of orange and white L. pertusa showed that the mucosal protein composition varies significantly with color phenotype, a pattern not reported prior to this study. Hence, to reduce variability from phenotype difference, L. pertusa white individuals only were selected to characterize in more detail the basal protein composition in mucus using liquid chromatography, mass spectrometry, mass spectrometry (LC-MS/MS). In total, 297 proteins were identified in L. pertusa mucus of unexposed coral individuals. Individuals exposed to drill cuttings in the range 2 to 12 mg/L showed modifications in coral mucus protein composition compared to unexposed corals. Although the results were somewhat inconsistent between individuals and require further validation in both the lab and the field, this study demonstrated preliminary encouraging results for discovery of protein markers in coral mucus that might provide more comprehensive insight into potential consequences attributed to anthropogenic stressors and may be used in future monitoring of coral health.

Description: The progressive bond breakage of artificially cemented sands induced by shear straining was investigated through conventional isotropically consolidated drained triaxial compression tests. Sand specimens were prepared with a low degree of cementation by adopting a chemical grout. Test results were interpreted in terms of two stress–dilatancy theories for cohesive-frictional materials proposed in literature. The influence of debonding on the stress–dilatancy behaviour of cemented sands was analysed with particular emphasis on the ‘delayed dilatancy’ phenomenon. A bonding degradation curve was determined for each test relating the interparticle cohesion (c) to the magnitude of the total plastic strain vector (εd) and a bond degradation rate factor (Dc) was assessed from each curve. The maximum value of interparticle cohesion (c0) before the onset of bond degradation under shearing was found to correspond with a sharp decrease in the soil stiffness of the specimens. The influence of the effective confining stress (p′c)on both c0 and Dc parameters gathered from each test was also ascertained.

Description: A simple unified critical state constitutive model for bonded geomaterials is presented in this paper. The model, which is called CASM-n, is an enhancement of an existing unified critical state model for reconstituted geomaterials (CASM). Characteristic behaviours of a bonded geomaterial such as the pre-yield higher strength and stiffness and the cohesive–frictional shearing mode in the post-yield region are taken into account and included in the reference model. The salient feature of the proposed model is the incorporation of cohesive component into the stress–dilatancy relationship. Consideration of the contribution of cohesion to plastic flow allows the modelling of delayed dilatancy and softening–contraction behaviour, which are two interesting phenomena observed in bonded geomaterials.

Description: Results from two recent field trials, onshore in the Alaska permafrost and in the Nankai Trough offshore Japan, suggest that natural gas could be produced from marine gas hydrate reservoirs at compatible yields and rates. However, both field trials were accompanied by different technical issues, the most striking problems resulting from un-predicted geomechanical behaviour, sediment destabilization and catastrophic sand production. So far, there is a lack of experimental data which could help to understand relevant mechanisms and triggers for potential soil failure in gas hydrate production, to guide model development for simulation of soil behaviour in large-scale production, and to identify processes which drive or, further, mitigate sand production. We use high-pressure flow-through systems in combination with different online and in situ monitoring tools (e.g. Raman microscopy, MRI) to simulate relevant gas hydrate production scenarios. Key components for soil mechanical studies are triaxial systems with ERT (Electric resistivity tomography) and high-resolution localstrain analysis. Sand production control and management is studied in a novel hollow-cylinder-type triaxial setup with a miniaturized borehole which allows fluid and particle transport at different fluid injection and flow conditions. We further apply a novel large-scale high-pressure flow-through triaxial test system equipped with μ-CT to evaluate soil failure modes and triggers relevant to gas hydrate production and slope stability. The presentation will emphasize an in-depth evaluation of our experimental approach, and it is our concern to discuss important issues of translating laboratory results to gas hydrate reservoirs in nature. We will present results from high-pressure flow-through experiments which are designed to systematically compare soil mechanical behaviour of gas hydrate-bearing sediments in relevant production scenarios focusing on depressurization and CO2 injection. Experimental datasets are analyzed based on numerical models which are able to simulate coupled process dynamics during gas hydrate formation and gas production.

Description: Manganese nodule coverage is estimated based on multi-beam and deep-towed video nodule survey profiles of about 1,700 km in the China Pioneer Area of Eastern Pacific. Two statistical equations for estimating nodule coverage are derived separately from the multi-beam normal incidence amplitude data and angular amplitude data based on theoretical analysis of influence factors on multi-beam amplitude data. Predictions generated by the normal incidence amplitude model fall within 5% of real nodule coverage, and theoretically calculated angular amplitude data fits well with the original multi-beam amplitudes at incident angles larger than 20° according to nodule coverage estimated from the deep-towed video data.

Description: Suspended particulate matter (SPM) causes most of the scattering in natural waters and thus has a strong influence on the underwater light field, and consequently on the whole ecosystem. Turbidity is related to the concentration of SPM which usually is measured gravimetrically, a rather time-consuming method. Measuring turbidity is quick and easy, and therefore also more cost-effective. When derived from remote sensing data the method becomes even more cost-effective because of the good spatial resolution of satellite data and the synoptic capability of the method. Turbidity is also listed in the European Union’s Marine Strategy Framework Directive as a supporting monitoring parameter, especially in the coastal zone. In this study, we aim to provide a new Baltic Sea algorithm to retrieve SPM concentration from in situ turbidity and investigate how this can be applied to satellite data. An in situ dataset was collected in Swedish coastal waters to develop a new SPM model. The model was then tested against independent datasets from both Swedish and Lithuanian coastal waters. Despite the optical variability in the datasets, SPM and turbidity were strongly correlated (r = 0.97). The developed model predicts SPM reliably from in situ turbidity (R2 = 0.93) with a mean normalized bias (MNB) of 2.4% for the Swedish and 14.0% for the Lithuanian datasets, and a relative error (RMS) of 25.3% and 37.3%, respectively. In the validation dataset, turbidity ranged from 0.3 to 49.8 FNU (Formazin Nephelometric Unit) and correspondingly, SPM concentration ranged from 0.3 to 34.0 g m–3 which covers the ranges typical for Baltic Sea waters. Next, the medium-resolution imaging spectrometer (MERIS) standard SPM product MERIS Ground Segment (MEGS) was tested on all available match-up data (n = 67). The correlation between SPM retrieved from MERIS and in situ SPM was strong for the Swedish dataset with r = 0.74 (RMS = 47.4 and MNB = 11.3%; n = 32) and very strong for the Lithuanian dataset with r = 0.94 (RMS = 29.5% and MNB = −1.5%; n = 35). Then, the turbidity was derived from the MERIS standard SPM product using the new in situ SPM model, but retrieving turbidity from SPM instead. The derived image was then compared to existing in situ data and showed to be in the right range of values for each sub-area. The new SPM model provides a robust and cost-efficient method to determine SPM from in situ turbidity measurements (or vice versa). The developed SPM model predicts SPM concentration with high quality despite the high coloured dissolved organic matter (CDOM) range in the Baltic Sea. By applying the developed SPM model to already existing remote sensing data (MERIS/Envisat) and most importantly to a new generation of satellite sensors (in particular OLCI on board the Sentinel-3), it is possible to derive turbidity for the Baltic Sea.

Description: Three new species of siphonophores (Rhodaliidae), a little known, uniquely benthic, family of Physonectae (Hydrozoa, Cnidaria), are described from specimens observed and collected at depths between 120 and 360 m off Indonesia and South Africa by the submersible JAGO. Special reference is made to in situ observations and video close‐ups that revealed detailed information on locomotory, fishing and feeding behaviour.

Description: The spatial variations in the elemental and stable carbon, nitrogen, and sulphur isotope composition of bladder wrack (Fucus vesiculosus) growing along the shore line of the semi-enclosed urbanized Kiel Fjord (western Baltic Sea) was investigated at more than 60 sites. The analyses of the carbon-nitrogen-sulphur (CNS) stoichiometry and C and N stable isotope signature of F. vesiculosus displayed substantial differences between the north-western and the south-eastern parts of the Kiel Fjord. Different size classes displayed in part differences in C:N and C:S ratios, and the carbon isotope composition, reflecting the impact of the boundary conditions during growth. Whereas the sulphur isotope composition was controlled by the assimilation of seawater sulphate, the carbon isotope composition reflected the difference in the composition of surface waters. The δ15N values of the organic tissue tend to be an integrated monitor of anthropogenic impacts on the fjord. Results are compared to the composition of surface waters.

Description: The fluttering shearwater (Puffinus gavia) is an abundant seabird endemic to breeding colonies in northern and central New Zealand. The species remains poorly studied, and here we present the first study to examine its breeding biology in detail. Fluttering shearwater nests were monitored daily from laying in September 2015 to fledging in January 2016 on Burgess Island (Mokohinau Islands group) in the outer Hauraki Gulf, northern New Zealand. Burrows were generally simple and non-branched. Eggs were laid over a period of 39 days with laying peaking 12th September. Incubation was 50 ± 3.7 days and chicks fledged after an average of 74 ± 4.3 days, from late December to the end of January. Chick development corresponds to the pattern observed for other Procellariiformes, gaining body mass rapidly to a maximum of 115% of adult mass, and then losing weight until fledging. Chicks were fed most nights throughout chick-rearing, indicating adult birds have access to a stable food supply close to the colony. Breeding success was 63.8% and similar to other Puffinus species. This study provides baseline biological data for a poorly studied, yet common, New Zealand endemics seabird. The obtained new information will allow for further ecological investigations and improved conservation management for the species

Description: Chemical examination of the fermentation broth of a sponge-associated fungus Trichoderma harzinum HMS-15-3 led to the isolation of four pairs of new C13 lipid enantiomers namely harzianumols A–H (1a–4b). Their structures were elucidated on the basis of extensive spectroscopic (IR, MS, 1D, and 2D NMR) data analysis, including the modified Mosher's method for the assignment of their absolute configurations. The new compounds were evaluated for antihyperlipidemic effects in HepG2 cells.

Description: This study aimed to differentiate local and migratory spawning groups of the shortfin squid Illex argentinus caught in Brazilian waters using geometric and traditional morphometric methods and size-selective processes. The back-calculated length distributions reconstructed from daily growth increments deposited in the gladius allowed the identification of size-selective processes that may be related to different life history strategies. Landmark analysis on body shape (geometric morphometric) revealed that spawning groups presented significant ontogenetic variations in terms of body outline. In addition, traditional morphometric methods, based on multivariate analysis, associated juveniles of the expected migratory group (large size) and differentiated them from the local group individuals (small size). The changes in form, probably linked to the environmental gradients experienced by individuals throughout ontogeny, were interpreted as adaptations to improve swimming capacity. Migrant individuals have being differentiated from the smaller sizes group by body characteristics (broad fins, elongated and thicker mantle), which may increase the ability to perform long migrations.

Description: We investigated the biogeochemical constituents, microbial communities and functional genes (mcr and dsr) associated with anaerobic methane oxidation and sulfate reduction, and metabolic activities by sulfate reduction in the sulfate–methane transition zone (SMTZ) of gas-hydrate-bearing sediment of the Ulleung Basin in the East Sea. Maxima in the sulfate reduction rate (12.6 nmol cm¡3 d¡1), CO concentration (83 mM), and gene abundances of dsrA (9.1 £ 106 copies cm¡3) and mcrA (11.6 £ 106 copies cm¡3) occurred in the SMTZ. The peaks of CO consistently found in the SMTZ suggested that CO is an intermediate metabolic product related to methane oxidation. Candidate division JS1, the predominant bacterial group that comprised 59.0–63.7% of the 16S rRNA gene sequences, was recognized as an important organic carbon oxidizer. Both Marine Benthic Group D (MBGD) and Marine Benthic Group B (MBGB), which constituted 40.8–52.9 and 10.3–43.9% of the 16S rRNA gene sequences, respectively, were the dominant archaeal groups. Analysis of functional gene diversity revealed that anaerobic methanotroph-1-related phylotypes appeared to be the major CH4 oxidizer, whereas Firmicutes-like group was a predominant sulfate reducer in the 0.8 mbsf in SMTZ with low SO42¡ concentration. Overall results indicated that JS1 and two archaeal groups (MBGB and MBGD) seem to play a significant role in carbon and elements cycles in the gas-hydrate-bearing subsurface sediment of the Ulleung Basin.

Description: Introduction: The combination of growing antimicrobial resistance with a dry pipeline has resulted in infections that can no longer be treated. Specific reasons have led to companies’ exit from the antibacterial space, however recent incentives are spurring interest to reinvigorate the pipeline. Areas covered: This article summarizes the available information on the discovery, developmental, and regulatory challenges in antibacterial development that have led to disinterest in the space, as well as ongoing incentives such as public-private partnerships and streamlined pathways to mend these challenges and bring new antibiotics to patients in need. Expert commentary: Clinicians should not only understand the reasons for the decline in antibiotic development that have resulted in the dry pipeline, but also the ongoing initiatives in place to build an appropriate supply. Doing so will result in greater appreciation and prudent use of these life-saving drugs when they become available.

Description: Data from New Zealand and northern Victoria Land, Antarctica, indicate that the Cambrian Takaka Terrane intra-oceanic arc/backarc assemblage and the Bowers Terrane intra-oceanic arc/back-arc assemblage were accreted to the Gondwana margin by the Late Cambrian. Compelling similarities between the arc rocks and the immediate post-arc sediments firmly place the two regions in the same tectonic framework and imply close paleogeographic proximity. Currently, the Ross Orogen is thought to be the result of sinistral oblique convergence with west-directed subduction, and accretion of the arc assemblages is attributed to closure of backarc basins. Syntectonic fluvial conglomerates in both regions attest to the development of fluvial systems draining both the accreted arc and the contemporaneous continental margin arc. Trilobite faunas indicate that fluvial sedimentation commenced earlier in New Zealand than in northern Victoria Land. In the context of the widely accepted sinistral oblique convergence model, these data suggest an original position for New Zealand to the south of northern Victoria Land, probably in the region of the southern Ross Sea.

Description: Sediments of upwelling regions off Namibia, Peru, and Chile contain dense populations of large nitrate-storing sulfide-oxidizing bacteria, Thiomargarita, Beggiatoa, and Thioploca. Increased contents of monounsaturated C16 and C18 fatty acids have been found at all stations studied, especially when a high density of sulfide oxidizers in the sediments was observed. The distribution of lipid biomarkers attributed to sulfate reducers (10MeC16:0 fatty acid, ai-C15:0 fatty acid, and mono-O-alkyl glycerol ethers) compared to the distribution of sulfide oxidizers indicate a close association between these bacteria. As a consequence, the distributions of sulfate reducers in sediments of Namibia, Peru, and Chile are closely related to differences in the motility of the various sulfide oxidizers at the three study sites. Depth profiles of mono-O-alkyl glycerol ethers have been found to correlate best with the occurrence of large sulfide-oxidizing bacteria. This suggests a particularly close link between mono-O-alkyl glycerol ether-synthesizing sulfate reducers and sulfide oxidizers. The interaction between sulfide-oxidizing bacteria and sulfate-reducing bacteria reveals intense sulfur cycling and degradation of organic matter in different sediment depths.

Description: This paper reviews aspects of the biology of copepods infecting marine fish commercially cultured at fish farms or held as broodstock at governmental hatcheries in Japan. In total, 20 species of parasitic copepods have been reported from these fish: they are mostly caligids (12 spp.), followed by lernaeopodids (4 spp.), pennellid (1 sp.), chondracanthid (1 sp.), taeniacanthid (1 sp.), and unidentified species (1 sp.). The identified copepods are: Caligus fugu, C. lagocephalus, C. lalandei, C. latigenitalis, C. longipedis, C. macarovi, C. orientalis, C. sclerotinosus, C. spinosus, Lepeophtheirus longiventralis, L. paralichthydis, L. salmonis (Caligidae); Alella macrotrachelus, Clavella parva, Parabrachiella hugu, P. seriolae (Lernaeopodidae); Peniculus minuticaudae (Pennellidae); Acanthochondria priacanthi (Chondracanthidae); and Biacanthus pleuronichthydis (Taeniacanthidae). The fish recorded as hosts include carangids (4 spp.), sparids (2 spp.), monacanthids (2 spp.), salmonids (2 spp.), scombrid (1 sp.), tetraodontid (1 sp.), pleuronectid (1 sp.), paralichthyid (1 sp.), and trichodontid (1 sp.). Only five species (C. orientalis, L. longiventralis, L. salmonis, C. parva and A. priacanthi) parasitize farmed fish in subarctic waters, while all other species (15 spp.) infect farmed fish in temperate waters. No information is yet available on copepods from fish farmed in subtropical waters. Three species of Caligus (C. fugu, C. sclerotinosus and C. longipedis) are serious pests in aquaculture of Japanese pufferfish (Takifugu rubripes), red seabream (Pagrus major), and striped jaw (Pseudocaranx dentex), respectively. Due to its one-host life cycle that is unique among pennellids, P. minuticaudae is considered as a potentially serious pest with further development of aquaculture of its filefish hosts, threadsail filefish (Stephanolepis cirrhifer) and black scraper (Thamnaconus modestus). Infection of farmed salmonids by L. salmonis is not a serious problem in Japan

Description: A variety of methods were successfully applied to examine the efficacy of a modular ballast water system according to the standards as adopted by the International Maritime Organization. The ballast water treatment system had a capacity of 530 m3 h−1 consisted of a pump system, a hydrocyclone, a 50 μm mesh-size self-cleaning filter and an installation for the addition of a chemical disinfectant (PERACLEAN® Ocean). The land-based testing facility used natural sea water of high turbidity during the spring phytoplankton bloom. The mesozooplankton fraction was inspected with a standard binocular. Larger zooplankton were effectively removed with the filter; the smaller sized fraction containing larvae and nauplia were killed after chemical treatment. The phytoplankton component was monitored using flow cytometry. The huge colonies of the phytoplankton Phaeocystis globosa were disrupted in the hydrocyclone liberating the colony cells which passed as single cells through the filter. These cells remained viable but were finally killed in the secondary (chemical) step. Bacteria also passed all mechanical treatment steps unharmed but were killed in the final step. Viability tests with SYTOX Green, which were specifically designed for phytoplankton, showed that mechanical treatment did not affect the percentage of viable cells a short-term, but after several hours the viable cell counts dropped down to 70%. Phytoplankton cells recovered within a single day and formed a new dense bloom rapidly. The bacteriostatic component of the chemical disinfectant (H2O2) remained present for several days preventing regrowth of bacteria for up to 15 days after addition. In conclusion, the IMO standards were met using the modular ballast water treatment unit and the applied instruments and assays were effective and rapid tools to qualify and quantify the organisms present as well as their viability.

Description: A simple method for initializing coupled general circulation models (CGCMs) using only sea surface temperature (SST) data is comprehensively tested in an extended set of ensemble hindcasts with the Max-Planck-Institute (MPI) climate model, MPI-OM/ECHAM5. In the scheme, initial conditions for both atmosphere and ocean are generated by running the coupled model with SST nudged strongly to observations. Air–sea interaction provides the mechanism through which SST influences the subsurface. Comparison with observations indicates that the scheme is performing well in the tropical Pacific. Results from a 500-yr control run show that the model's El Niño Southern Oscillation (ENSO) variability is quite realistic, in terms of strength, structure and period. The hindcasts performed were six months long, initiated four times per year, consisted of nine ensemble members, and covered the period 1969–2001. The ensemble was generated by only varying atmospheric initial conditions, which were sampled from the initialization run to capture intraseasonal variability. At six-month lead, the model is able to capture all the major ENSO extremes of the period. However, because of poor sampling of ocean initial conditions and model deficiencies, the ensemble-mean anomaly correlation skill for Niño3 SST is only 0.6 at six-month lead. None the less, the results presented here demonstrate the potential of such a simple scheme, and provide a simple method by which SST information may be better used in more complex initialization schemes.

Description: The Soil Moisture and Ocean Salinity (SMOS) mission observes brightness temperatures at a low microwave frequency of 1.4 GHz (L-band) with a daily coverage of the polar regions. L-band radiometry has been shown to provide information on the thickness of thin sea ice. Here, we apply a new emission model that has previously been used to investigate the impact of snow on thick Arctic sea ice. The model has not yet been used to retrieve ice thickness. In contrast to previous SMOS ice thickness retrievals, the new model allows us to include a snow layer in the brightness temperature simulations. Using ice thickness estimations from satellite thermal imagery, we simulate brightness temperatures during the ice growth season 2011 in the northern Baltic Sea. In both the simulations and the SMOS observations, brightness temperatures increase by more than 20 K, most likely due to an increase of ice thickness. Only if we include the snow in the model, the absolute values of the simulations and the observations agree well (mean deviations below 3.5 K). In a second comparison, we use high-resolution measurements of total ice thickness (sum of ice and snow thickness) from an electromagnetic (EM) sounding system to simulate brightness temperatures for 12 circular areas. While the SMOS observations and the simulations that use the EM modal ice thickness are highly correlated (r2=0.95), the simulated brightness temperatures are on average 12 K higher than observed by SMOS. This would correspond to an 8-cm overestimation of the modal ice thickness by the SMOS retrieval. In contrast, if the simulations take into account the shape of the EM ice thickness distributions (r2=0.87), the mean deviation between simulated and observed brightness temperatures is below 0.1 K.

Description: Marine calcifiers as a plankton functional type (PFT) are a crucial part of the global carbon cycle, being responsible for much of the carbon export to the deep ocean entering via biological pathways. Deep ocean carbon export through calcifiers is controlled by physiological, ecological, and biogeochemical factors. This paper describes the implementation of a calcifying phytoplankton PFT in the University of Victoria Earth System Climate Model, version 2.9 (UVic ESCM), and mechanistic improvements to the representation of model carbon export (a full calcite tracer, carbonate chemistry dependent calcite dissolution rates, and a ballasting scheme). An iterative method for stabilizing and tuning the biogeochemistry is furthermore described. The UVic ESCM now fills a niche in Earth system modelling that was previously unoccupied in that it is relatively inexpensive to run, yet resolves the complete Earth system carbon cycle including prognostic calcium carbonate and a separate phytoplankton calcifier PFT. The model is now well suited to testing feedbacks between the carbonate and carbon cycles and the climate system as transient simulations. The modifications described improve the UVic ESCM's mechanistic realism without compromising performance with respect to observed carbon and nutrient fluxes. Primary production, export production, particulate organic carbon, and calcite fluxes all fall within independently observed estimates.

Description: The current state of development of an interactive electronic identification key for females of the marine pelagic microcopepod family Oncaeidae is presented. To date, 112 species have been described, allocated to seven genera (Archioncaea, Conaea, Epicalymma, Monothula, Oncaea s.str., Spinoncaea, and Triconia) and 16 species groups within the speciose paraphyletic taxon Oncaea s.l. Only two species of Oncaea s.l. show an uncertain group status. Of all described oncaeids, seven species are not yet included in the key due to inadequacies in morphological description and/or lack of type material for re-examination. In addition, seven morphologically distinct species and two new species groups are considered that have not yet been formally described. The key uses easily distinguishable morphological characters as far as possible (e.g. body size, proportional lengths of urosomites, length of P5 exopod) and includes more difficult characters (e.g. spine counts on swimming legs, proportional spine lengths) where required for unequivocal identification. The characters used in the key allow the identification of genera and species groups by investigators with limited taxonomic experience; species separation in most cases requires some advanced dissection skills. Potential identification problems caused by difficulties discerning minute morphological features or intraspecific morphometric variability are discussed. The present version of the identification key still requires complementary illustrations of character states and inclusion of fact sheets summarizing the characteristics of each species with notes on taxonomic uncertainties and links to databases providing zoogeographical and genetic information.

Description: Meteorite impacts cause conversion of kinetic energy into thermal energy. Part of this thermal energy is used to form a melt sheet, part is dissipated to heat the target rocks and these together with the hot rocks that elastically rebound from the depth of several kilometres (central uplift) activate hydrothermal circulation. Impact-generated hydrothermal systems have been documented from several impact structures world-wide. Three Australian examples—Shoemaker, Woodleigh and Yarrabubba—provide evidence of hydrothermal fluid flow both within and around the structures. Field observations, and petrographic and geochemical data suggest a common evolutionary trend of post-impact hydrothermal activity from early high-temperature alkali metasomatism to a later lower temperature H + metasomatism, resulting in the overprinting by hydrous mineral assemblages. Hydrothermal systems activated by meteorite-impact events are important because they may also form economic mineral deposits, as is documented for several impact structures in the world. A working model of hydrothermal circulation in terrestrial impact structures posits two main stages: (i) initial high-temperature fluids percolate downward causing widespread alkali metasomatism of the shattered target rocks below the melt sheet, resulting in their modification to rocks of syenitic affinity; and (ii) inflow of meteoric water and progressive cooling of the melt sheet leads to a lower temperature stage, in which hydrothermal fluid flow tends to move upward, resulting in mineral assemblages and alteration patterns that resemble those of epithermal systems. In addition, these fluids can discharge at the surface as hot springs.

Description: Some 290 species of squids comprise the order Teuthida that belongs to the molluscan Class Cephalopoda. Of these, about 30-40 squid species have substantial commercial importance around the world. Squid fisheries make a rather small contribution to world landings from capture fisheries relative to that of fish, but the proportion has increased steadily over the last decade, with some signs of recent leveling off. The present overview describes all substantial squid fisheries around the globe. The main ecological and biological features of exploited stocks, and key aspects of fisheries management are presented for each commercial species of squid worldwide. The history and fishing methods used in squid fisheries are also described. Special attention has been paid to interactions between squid fisheries and marine ecosystems including the effects of fishing gear, the role of squid in ecosystem change induced by overfishing on groundfish, and ecosystem-based fishery management.

Description: Sea ice leads play an essential role in ocean-ice-atmosphere exchange, in ocean circulation, geochemistry and in ice dynamics. Their precise detection is crucial for altimetric estimations of sea ice thickness and volume. This study evaluates the performance of the SARAL/AltiKa altimeter to detect leads and to monitor their spatio-temporal dynamics. We show that a pulse peakiness parameter (PP) used to detect leads by Envisat RA-2 and ERS-1,-2 altimeters is not suitable because of saturation of AltiKa return echoes over the leads. The signal saturation results in loss of 6–10% of PP data over sea ice. We propose a different parameter: maximal power of waveform, and define the threshold to discriminate the leads. Our algorithm can be applied from December until May. It detects well the leads of small and medium size: from 200 m to 3–4 km. So, the combination of the high-resolution altimetric estimates with low-resolution thermal infra-red or radiometric lead fraction products could enhance the capability of remote sensing to monitor sea ice fracturing.

Description: The impact of fire on daily discharges from two mountainous basins located in the permafrost region of Eastern Siberia; the Vitimkan (969 km2) and Vitim rivers (18 200 km2), affected by fire over 78% and 49% of their areas, respectively, in 2003 was investigated. The results of hydrological and meteorological data analysis suggested that the Vitimkan River basin had a rapid and profound hydrological response to wildfire in 2003 expressed through an increased summer flow of 41% (133 mm). Conversely, the larger Vitim River basin showed no significant changes in discharges after the fire. The parameters of the process-based hydrological model Hydrograph were estimated for pre-fire conditions. The results of runoff simulations conducted for continuous pre-fire periods of 1966–2002 and 1970–2002 for the Vitimkan and Vitim River basins respectively on a daily time step showed satisfactory agreement with the observed flow series of both basins. Significant underestimation of precipitation and its poor representativeness for mountainous watersheds was revealed as the main cause of observed and simulated flow discrepancies, especially for high flood events. The set of dynamic parameters was developed based on data analysis and post-fire landscape changes as derived from a literature review. The model was applied to investigate the processes in the soil column and their effect on runoff formation during the post-fire period. The new set of model parameters implied intensification of soil thaw, reduction of infiltration rate and evapotranspiration, and increase of upper subsurface flow fraction in summer flood events following the fire. According to modelling results, the post-fire thaw depth exceeded the pre-fire thaw depth by 0.4–0.7 m. Total evapotranspiration reduced by 40% in summer months, while surface flow increased almost 2.5 times during maximum flood events.

Description: Macroalgae, especially perennial species, are exposed to a seasonally variable fouling pressure. It was hypothesized that macroalgae regulate their antifouling defense to fouling pressure. Over one year, the macrofouling pressure and the chemical anti-macrofouling defense strength of the brown algae Fucus vesiculosus and Fucus serratus were assessed with monthly evaluation. The anti-macrofouling defense was assessed by means of surface-extracted Fucus metabolites tested at near-natural concentrations in a novel in situ bioassay. Additionally, the mannitol content of both Fucus species was determined to assess resource availability for defense production. The surface chemistry of both Fucus species exhibited seasonal variability in attractiveness to Amphibalanus improvisus and Mytilus edulis. Of this variability, 50–60% is explained by a sinusoidal model. Only F. vesiculosus extracts originating from the spring and summer significantly deterred settlement of A. improvisus. The strength of macroalgal antifouling defense did not correlate either with in situ macrofouling pressure or with measured mannitol content, which, however, were never depleted.

Description: We used our novel and programmable Porpoise Alarm (PAL, patd.) to synthesize life-like, electronic harbour porpoise communication signals based on those described for captive animals. In the Little Belt, Denmark, we employed PAL (source level 158 ± 1 dB p–p re 1 μPa@1 m; centroid frequency 133 ± 8.5 kHz) to synthesize three aggressive click train types termed “A”, “F3” and “M1” to naive, free-living harbour porpoises. Via theodolite tracking (372 h of total visual effort spread over 10 expeditions) we found that, depending on signal type, porpoises either avoid or become attracted to PAL: Signal types “A” and “F3” are slight deterrents, porpoises increasing minimum range (+23 to 32 m, respectively), whereas “M1” attracts porpoises, reducing range (by − 29 m). As determined via archival acoustic detectors (AADs), both signals “F3” and “M1” led the animals to significantly intensify their click rate (by +10% and 68%, respectively) while signal “A” led to a significant reduction ( − 59%). We propose that equipping fishing gear with PAL emitting signal “F3” could potentially reduce porpoise by-catch by increasing (1) awareness through enhanced echolocation and (2) distance to the nets. Detection probability and radius of PAL/AAD tandems could be improved by emitting signal “M1” to focus porpoise echolocation signals on the AAD. The signal may also be useful in luring animals away from hazards, which may be helpful for conservation measures prior to the onset of harmful acoustic activities such as pile-driving, seismic exploration or ammunition clearance.

Description: Rarity of species is often considered to set priorities for biodiversity conservation. Less abundant species are expected to be at higher risk of extinction and make significant contribution to food web functioning. However, the relationship between species abundance and position in food webs is still unclear. Here we tested possible correlations between species abundance and structural position in Prince William Sound food web. Species abundance was inferred from biomass data and structural position was characterized by 13 centrality indices. We found that less abundant species have higher trophic positions and display more generalist feeding strategies. However, positive correlations link most of the centrality indices to population size. Thus, being locally rare translates into more peripheral food web positions and implies marginal roles in the spread of indirect effects. Species characterized by largest population size are responsible for the transfer of largest amounts of biomass and regulate the transmission of indirect effects. Less abundant species are of marginal structural importance and are exposed to impacts mediated by larger populations. In Prince William Sound ecosystem, rarity is associated with critical food web positions and does not simply reflect a marginal contribution to biodiversity. We suggest that knowing the food web position of rare species might help to formulate more effective, system-level solutions for their conservation, rather than simply focusing on the direct treatment of symptoms.

Description: Meteorite impacts cause conversion of kinetic energy into thermal energy. Part of this thermal energy is used to form a melt sheet, part is dissipated to heat the target rocks and these together with the hot rocks that elastically rebound from the depth of several kilometres (central uplift) activate hydrothermal circulation. Impact-generated hydrothermal systems have been documented from several impact structures world-wide. Three Australian examples—Shoemaker, Woodleigh and Yarrabubba—provide evidence of hydrothermal fluid flow both within and around the structures. Field observations, and petrographic and geochemical data suggest a common evolutionary trend of post-impact hydrothermal activity from early high-temperature alkali metasomatism to a later lower temperature H+ metasomatism, resulting in the overprinting by hydrous mineral assemblages. Hydrothermal systems activated by meteorite-impact events are important because they may also form economic mineral deposits, as is documented for several impact structures in the world. A working model of hydrothermal circulation in terrestrial impact structures posits two main stages: (i) initial high-temperature fluids percolate downward causing widespread alkali metasomatism of the shattered target rocks below the melt sheet, resulting in their modification to rocks of syenitic affinity; and (ii) inflow of meteoric water and progressive cooling of the melt sheet leads to a lower temperature stage, in which hydrothermal fluid flow tends to move upward, resulting in mineral assemblages and alteration patterns that resemble those of epithermal systems. In addition, these fluids can discharge at the surface as hot springs.

Description: Shells of the blue mussel Mytilus edulis remain free of fouling organisms as long as they possess an intact periostracum, and a multiple antifouling defence that comprises a ripple-like microtopography and the production of chemical antifouling compounds has been suggested previously. This study investigates the chemical defence strategy of blue mussels for the first time. Six crude extracts of the periostracum of intact shells were made using solvents of increasing polarity. These extracts were tested against common fouling organisms in laboratory based bioassays. Non-polar and moderately polar fractions showed the highest activities: the diethyl ether fraction strongly inhibited attachment of Balanus amphitrite cyprids and the marine bacteria Cobetia marina and Marinobacter hydrocarbonoclasticus. Attachment of the benthic diatom Amphora coffeaeformis was significantly reduced by the dichloromethane extract, whereas both ethyl acetate and diethyl ether fractions slowed diatom growth. These results provide the first evidence of surface bound compounds that may moderate surface colonisation.

Description: The regional dust model system LM-MUSCAT-DES was developed in the framework of the SAMUM project. Using the unique comprehensive data set of near-source dust properties during the 2006SAMUMfield campaign, the performance of the model system is evaluated for two time periods in May and June 2006. Dust optical thicknesses, number size distributions and the position of the maximum dust extinction in the vertical profiles agree well with the observations. However, the spatio-temporal evolution of the dust plumes is not always reproduced due to inaccuracies in the dust source placement by the model. While simulated winds and dust distributions are well matched for dust events caused by dry synoptic-scale dynamics, they are often misrepresented when dust emissions are caused by moist convection or influenced by small-scale topography that is not resolved by the model. In contrast to long-range dust transport, in the vicinity of source regions the model performance strongly depends on the correct prediction of the exact location of sources. Insufficiently resolved vertical grid spacing causes the absence of inversions in the model vertical profiles and likely explains the absence of the observed sharply defined dust layers.

Description: Tropical cyclones (TC) under different climate conditions in the Northern Hemisphere have been investigated with the Max Planck Institute (MPI) coupled (ECHAM5/MPI-OM) and atmosphere (ECHAM5) climate models. The intensity and size of the TC depend crucially on resolution with higher wind speed and smaller scales at the higher resolutions. The typical size of the TC is reduced by a factor of 2.3 from T63 to T319 using the distance of the maximum wind speed from the centre of the storm as a measure. The full three-dimensional structure of the storms becomes increasingly more realistic as the resolution is increased. For the T63 resolution, three ensemble runs are explored for the period 1860 until 2100 using the IPCC SRES scenario A1B and evaluated for three 30 yr periods at the end of the 19th, 20th and 21st century, respectively. While there is no significant change between the 19th and the 20th century, there is a considerable reduction in the number of the TC by some 20% in the 21st century, but no change in the number of the more intense storms. Reduction in the number of storms occurs in all regions. A single additional experiment at T213 resolution was run for the two latter 30-yr periods. The T213 is an atmospheric only experiment using the transient sea surface temperatures (SST) of the T63 resolution experiment. Also in this case, there is a reduction by some 10% in the number of simulated TC in the 21st century compared to the 20th century but a marked increase in the number of intense storms. The number of storms with maximum wind speeds greater than 50 m s−1 increases by a third. Most of the intensification takes place in the Eastern Pacific and in the Atlantic where also the number of storms more or less stays the same. We identify two competing processes effecting TC in a warmer climate. First, the increase in the static stability and the reduced vertical circulation is suggested to contribute to the reduction in the number of storms. Second, the increase in temperature and water vapour provide more energy for the storms so that when favourable conditions occur, the higher SST and higher specific humidity will contribute to more intense storms. As the maximum intensity depends crucially on resolution, this will require higher resolution to have its full effect. The distribution of storms between different regions does not, at first approximation, depend on the temperature itself but on the distribution of the SST anomalies and their influence on the atmospheric circulation. Two additional transient experiments at T319 resolution where run for 20 yr at the end of the 20th and 21st century, respectively, using the same conditions as in the T213 experiments. The results are consistent with the T213 study. The total number of TC were similar to the T213 experiment but were generally more intense. The change from the 20th to the 21st century was also similar with fewer TC in total but with more intense cyclones.

Description: Screening for pathogenic micro-organisms is an essential component of translocation-based conservation management. While there are some data on pathogens in New Zealand passerines, little is known about the distribution and prevalence of pathogens infecting New Zealand Psittaciformes. We conducted a survey for pathogens of the vulnerable New Zealand endemic red-crowned parakeet Cyanoramphus novaezelandiae in two wild populations (Little Barrier Island and Raoul Island), and in a translocated population (Tiritiri Matangi Island). A total of 101 cloacal samples were tested for Salmonella and Yersinia. Of these, 82 samples were also tested for Campylobacter. None of these microorganisms were detected. Although our sampling effort was insufficient to detect a low prevalence of Campylobacter, modelling of minimum detectable prevalence of Salmonella and Yersinia indicates that these micro-organisms would have been detected if present as common or chronic conditions of red-crowned parakeets at these sites.

Description: This review covers 214 marine natural compounds and 23 of their synthetic analogs, which were discovered and/or synthesized from mid-2009 to August 2014. The antifouling (AF) compounds reported have medium to high bioactivity (with a threshold of EC(50) 〈 15.0 mg ml(-1)). Among these compounds, 82 natural compounds were identified as new structures. All the compounds are marine-derived, demonstrating that marine organisms are prolific and promising sources of natural products that may be developed as environmentally friendly antifoulants. However, this mini-review excludes more than 200 compounds that were also reported as AF compounds but with rather weak bioactivity during the same period. Also excluded are terrestrial-derived AF compounds reported during the last five years. A brief discussion on current challenges in AF compound research is also provided to reflect the authors' own views in terms of future research directions.

Description: The White Shrimp (Litopenaeus vannamei) is reared in several types of systems. Changes in pH in some can produce sub-lethal effects that result in poor growth and survival. The aim of the present study was to detennine the acidic and basic pH 50% lethal limits after 96 h exposure (pH50_96 h) and to evaluate the sub-lethal effects of pH on oxidative stress parameters in juvenile L. vannamei. The experimental design comprised nine treatments with three replications of each. The nine treatments consisted of pH 10.5, 10.0, 9.5, 9.0, 7.0, 5.0, 4.5, 4.0 and 3.5. The treatment at 7.0 was used as the control. The sub~lethal pH tests were: basic pH 9.5, neutral pH 7.0 (control) and acidio pH 4.5. For each experimental pH and sampling point, the hemolymph of six shrimp was collected to determine catalase and glutathione S-transferase's activities and total antioxidant capacity. The values of lethat pH50 at 24, 48, 72 and 96 h for juvenile L. vannamei had basic pH values equal to 9.82, 9.62, 9.59 and 9.58 and acidic pH values of 3.86, 3.92, 3.94 and 4.04, respectively. The shrimp exposed to basic and acidic pH levels showed antioxidant responses with changes in antioxidant activity.

Description: Abstract: Context: Some Ajuga L. (Lamiaceae) species are traditionally used for the treatment of malaria, as well as fever, which is a common symptom of many parasitic diseases. Objective: In the continuation of our studies on the identification of antiprotozoal secondary metabolites of Turkish Lamiaceae species, we have investigated the aerial parts of Ajuga laxmannii. Materials and methods: The aerial parts of A. laxmannii were extracted with MeOH. The H2O subextract was subjected to polyamide, C18-MPLC and SiO2 CCs to yield eight metabolites. The structures of the isolates were elucidated by NMR spectroscopy and MS analyses. The extract, subextracts as well as the isolates were tested for their in vitro antiprotozoal activities against Plasmodium falciparum, Trypanasoma brucei rhodesiense, T. cruzi and Leishmania donovani at concentrations of 90–0.123 μg/mL. Results: Two iridoid glycosides harpagide (1) and 8-O-acetylharpagide (2), three o-coumaric acid derivatives cis-melilotoside (3), trans-melilotoside (4) and dihydromelilotoside (5), two phenylethanoid glycosides verbascoside (6) and galactosylmartynoside (7) and a flavone-C-glycoside, isoorientin (8) were isolated. Many compounds showed moderate to good antiparasitic activity, with isoorientin (8) displaying the most significant antimalarial potential (an IC50 value of 9.7 μg/mL). Discussion and conclusion: This is the first report on the antiprotozoal evaluation of A. laxmannii extracts and isolates. Furthermore, isoorientin and dihydromelilotoside are being reported for the first time from the genus Ajuga.

Description: Cephalopods play a significant role in coastal and oceanic ecosystems, both as consumers of invertebrates and small fish and as the prey of some fish, seabirds and marine mammals and other large predators. Approximately 30 species of cephalopod have been recorded in the north-eastern Atlantic and adjacent waters, including 18 teuthid (squid), seven sepiolid (bobtail), three sepiid (cuttlefish) and 10 octopod (octopus) species. A number of these are exploited commercially and support important target and by-catch fisheries in Western Europe. During the past decade, annual landings of cephalopods from the north-eastern Atlantic (International Council for the Exploration of the Sea [ICES] area) have ranged from 40,000 to 55,000 t, including substantial catches of long-fin (loliginid) squid (7000–11,000 t per annum), short-fin (ommastrephid) squid (3000–10,000 t), cuttlefish (including sepiolids; 16,000–24,000 t) and octopods (12,000–18,000 t). The most important exploited species in the north-eastern Atlantic are Eledone cirrhosa, Illex coindetii, Loligo forbesi, Loligo vulgaris, Octopus vulgaris, Todarodes sagittatus, Todaropsis eblanae and Sepia officinalis. Other species including Alloteuthis subulata, Gonatus fabricii and certain sepiolids, appear to be abundant and may be marketable. Cephalopods tend to rapidly concentrate heavy metals and other toxic substances in their tissues and this plays an important role in the bioaccumulation of these pollutants in marine predators as well as having implications for human consumption. High levels of cadmium and mercury are often recorded in cephalopod tissues. Another important environmental issue concerns the potential impact of widespread human activity on cephalopod spawning areas, particularly bottom-fishing operations but also shipping, and oil exploration and production. In contrast to many finfish species that spawn annually over a number of years, most cephalopods live only 1–2 yr and die after spawning. Therefore, failure to reproduce and recruit adequately in any given year may seriously impact the long-term viability of cephalopod stocks. Climate change is expected to have a significant effect on many species in the north-eastern Atlantic. This review provides a detailed account of the zoogeography, biology and ecology of cephalopods in the north-eastern Atlantic, on a species-by-species basis. Important economic, ecological and conservation issues affecting cephalopods in this area are also discussed

Description: Greywacke, argillite, greyschist and hypabyssal igneous rocks have been obtained from an Ocean Drilling Program core on the Queensland Plateau and from xenoliths in a volcanic breccia dredged from the crest of the Lord Howe Rise. Low to intermediate detrital quartz contents, 260 – 240 Ma K – Ar ages, and only moderately radiogenic Sr and Nd isotope compositions, suggest a correlation with the New England Orogen of eastern Australia, rather than with Australia's Lachlan Orogen or other adjacent geological provinces. Our results indicate that the New England Orogen terranes continue towards New Zealand at least as far as the southern Lord Howe Rise. The projected offshore boundaries of the major east Australian orogens are now known with more confidence, and do not appear to require any major cross-orogen offsets.

Description: Sea level changes in the Baltic Sea are dominated by internal, short-term variations that are mostly caused by the ephemeral nature of atmospheric conditions over the Baltic area. Tides are small and their influence decreases from western parts of the Baltic Sea to the Baltic Proper. Superimposed to the large short-term sea level changes (up to few decimeters from day to day) are seasonal and interannual variations (centimeters to decimeters). This study focuses on the comparison of sea surface heights obtained from observations and from a high resolution oceanographic model of the Baltic Sea. From this comparison, the accuracy of the modeled sea surface variations is evaluated, which is a necessary precondition for the further use of the oceanographic model in geodetic applications. The model reproduces all observed Baltic sea level variations very reliably with an accuracy of 5 to 9 cm (rms) for short-term variations (up to 2 months) and 8 cm (rms) for long-term variations (〉2 months). An additional improvement of the model can be attained by including long-period sea level variations of the North Sea. The model performs well also in the case of extreme sea level events, as is shown for a major storm surge that occurred at the southern coast of the Baltic Sea in November 1995.

Description: The properties of the body surface play a crucial role in most interactions of marine organisms. Critical ecological properties such as drag, morphology, uptake and release of radiation and organic matter are linked to the body surface of an aquatic organism. The properties and functions of this interface may be modified substantially by the presence and activities of epibiotic communities. This, in turn, may lead to substantial modulation of the interactions between the organism bearing epiphytes and its environment, with consequences for the relative fitness of the host organism (basibiont) and its interactors, and ultimately, the structure and functioning of the assemblage. Epibiosis may act as an ecological lever via these indirect effects, greatly amplifying or buffering biotic and abiotic stress.

Description: Modern antifouling coatings use heavy metals and toxic organic molecules to prevent biofouling, the undesirable growth of marine organisms on man-made substrata. In an ongoing survey of deep-sea microorganisms aimed at finding low toxic antifouling metabolites, an actinomycete bacterium was isolated from the Pacific sediment at the depth of about 5000 m. The bacterium was closely related to Streptomyces fungicidicus (99% similarity) according to 16S ribosomal RNA sequence information. The spent culture medium of this bacterium inhibited barnacle larval attachment. Bioassay-guided fractionation was employed to isolate antifouling compounds. The ethyl acetate extract was fractionated by using an open silica gel column. Active fractions were further purified on a HPLC C18 column. Five diketopiperazines, cyclo-(L-Leu-L-Pro), cyclo-(L-Phe-L-Pro), cyclo-(L-Val-L-Pro), cyclo-(L-Trp-L-Pro), and cyclo-(L-Leu-L-Val) were isolated for the first time from a deep sea bacterium, and the structures of the compounds were elucidated by nuclear magnetic resonance spectroscopy and mass spectrometry. The pure diketopiperazines were tested for antilarval activity using the barnacle Balanus amphitrite. Effective concentrations that inhibited 50% larval attachment (EC50) after 24 h ranged from 0.10- 0.27 mM. The data suggest that diketopiperazines and other compounds from deep-sea bacteria may be used as novel antifoulants.

Description: The SAMUM field campaign in southern Morocco in May/June 2006 provides valuable data to study the emission, and the horizontal and vertical transports of mineral dust in the Northern Sahara. Radiosonde and lidar observations show differential advection of air masses with different characteristics during stable nighttime conditions and up to 5-km deep vertical mixing in the strongly convective boundary layer during the day. Lagrangian and synoptic analyses of selected dust periods point to a topographic channel from western Tunisia to central Algeria as a dust source region. Significant emission events are related to cold surges from the Mediterranean in association with eastward passing upper-level waves and lee cyclogeneses south of the Atlas Mountains. Other relevant events are local emissions under a distinct cut-off low over northwestern Africa and gust fronts associated with dry thunderstorms over the Malian and Algerian Sahara. The latter are badly represented in analyses from the European Centre for Medium–Range Weather Forecasts and in a regional dust model, most likely due to problems with moist convective dynamics and a lack of observations in this region. This aspect needs further study. The meteorological source identification is consistent with estimates of optical and mineralogical properties of dust samples.

Description: From September to October 2002, shallow drilling, using the submersible (5 m) Rockdrill of the British Geological Survey and the German R/V Sonne revealed critical information on the subsurface nature of two distinct hydrothermal systems in the New Ireland fore-arc and the Manus Basin of Papua New Guinea. Drilling at Conical Seamount significantly extends the known surface extent of the previously discovered vein-style gold mineralization (up to 230 g/t Au) at this site. Drilling the conventional PACMANUS volcanic-hosted massive sulfide deposit recovered complexly textured massive sulfide with spectacular concentrations of gold in several core sections including 0.5 m @ 28 g/t Au, 0.35 m @ 30 g/t Au, and 0.20 m @ 57 g/t Au. Shallow drilling is a fast and cost efficient method that bridges the gap between surface sampling and deep (ODP) drilling and will become a standard practice in the future study of seafloor hydrothermal systems and massive sulfide deposits.

Description: The importance of crustal fluid chemical composition in driving the marine deep subseafloor biosphere was examined in northeast Pacific ridge-flank sediments. At IODP Site U1301, sulfate from crustal fluids diffuses into overlying sediments, forming a transition zone where sulfate meets in situ-produced methane. Enhanced cell counts and metabolic activity suggest that sulfate stimulates microbial respiration, specifically anaerobic methane oxidation coupled to sulfate reduction. Cell counts and activity are also elevated in basement-near layers. Owing to the worldwide expansion of the crustal aquifer, we postulate that crustal fluids may fuel the marine deep subseafloor biosphere on a global scale.

Description: Barnacle cypris larvae respond to many cues when selecting a settlement site. The settlement of over a million larvae on tiles of different textures, orientations and densities of incumbent settlers was measured on the rocky intertidal at Great Cumbrae, Scotland. Half of the tiles were replaced every tide whereas the others simultaneously accumulated settlers. Factor effects varied on each tide, and converged in the accumulating deployment. Increasing incumbent density led to net loss of settlement, which was less probable on the textures on which fastest settlment occurred ('very fine'), and more probable on those on which settlement was slowest ('smooth'). More settlement occurred on down-facing orientations during daylight and vice versa. Cue ranks were non-linear, so a path analysis model quantified the relative influence of each factor. Gregariousness was the most influential cue measured, although unmeasured factors had greater effects, highlighting the complexity of settlement influences in this species.

Description: Fluorescence excitation-emission matrices showed the spectral signature of dissolved organic matter (DOM) downstream in the River Tet at all seasons corresponding to humic substances with maximum fluorescence emission em=420-460nm for excitations of ex=340-360nm and the occasional presence of tryptophan, tracer of anthropogenic pressure, spectroscopically identified by ex/em=310/350nm. A factorial discriminant analysis, performed using the parameters selected (ex/em pairs of wavelength), succeeded in a better discrimination of seasons than stations, and clearly showed the presence of two fluorophores. Fluorophore 1, with two absorption bands: ex=260-320nm and 330-390nm for em=440-500nm, and Fluorophore 2, with one absorption band: ex=300-360nm for em=410nm, which are attributed to humic acid (HA) and fulvic acid (FA), respectively. Variations of DOM relative contents downstream in the River Tet, according to seasons and stations, showed high amounts of DOM (pedogenic character) along the river in the humid period, with the highest value obtained in the mountain section. In contrast, in summer, the DOM contents were low (aquagenic character). Moreover, DOM presents a relatively constant composition with a percentage of FA ranging from 40% at the mountain station to 48% at the mouth river, whatever the season.

Description: The Red Sea has been recognized as a unique region to study the effects of ecohydrographic gradients at a basin-wide scale. Its gradient of temperature and salinity relates to the Indian Ocean monsoon and associated wind-driven transport of fertile and plankton-rich water in winter from the Gulf of Aden into the Red Sea. Subsequent evaporation and thermohaline circulation increase the salinity and decrease water temperatures toward the North. Compared with other ocean systems, however, relatively little is known about the zooplankton biodiversity of the Red Sea and how this relates to Red Sea latitudinal gradients. Among the most abundant zooplankton taxa are Chaetognatha, which play an important role as secondary consumers in most marine food webs. Since Chaetognatha are sensitive to changes in temperature and salinity, we surmised latitudinal changes in their biodiversity, community structure and diel variability along the coast of Saudi Arabia. Samples were collected at nine coral reefs spanning approximately 1500 km, from the Gulf of Aqaba in the northern Red Sea to the Farasan Archipelago in the southern Red Sea. Thirteen Chaetognatha species belonging to two families (Sagittidae and Krohnittidae) were identified. Latitudinal environmental changes and availability of prey (i.e. Copepoda, Crustacea) altered Chaetognatha density and distribution. The cosmopolitan epiplanktonic Flaccisagitta enflata (38.1%) dominated the Chaetognatha community, and its abundance gradually decreased from South to North. Notable were two mesopelagic species (Decipisagitta decipiens and Caecosagitta macrocephala) in the near-reef surface mixed layers at some sites. This was related to wind-induced upwelling of deep water into the coral reefs providing evidence of trophic oceanic subsidies. Most Sagittidae occurred in higher abundances at night, whereas Krohnittidae were more present during the day. Chaetognatha with developing (stage II) or mature ovaries (stage III) were more active at night, demonstrating stage-specific diel vertical migration as a potential predator avoidance strategy.

Description: This study aimed to improve understanding of the strategies developed by the Mediterranean seaweed Taonia atomaria to chemically control bacterial epibiosis. An experimental protocol was optimized to specifically extract algal surface-associated metabolites by a technique involving dipping in organic solvents whilst the integrity of algal cell membranes was assessed by fluorescent microscopy. This methodology was validated using mass spectrometry-based profiles of algal extracts and analysis of their principal components, which led to the selection of methanol as the extraction solvent with a maximum exposure time of 15 s. Six compounds (A–F) were identified in the resulting surface extracts. Two of these surface-associated compounds (B and C) showed selective anti-adhesion properties against reference bacterial strains isolated from artificial surfaces while remaining inactive against epibiotic bacteria of T. atomaria. Such specificity was not observed for commercial antifouling biocides and other molecules identified in the surface or whole-cell extracts of T. atomaria.

Description: Since early 2008, an increasing number of countries have ratified an international treaty to ban the application of antifouling (AF) coatings based on organotin compounds (eg tributyltin (TBT) and triphenyltin). As a result, the demand for environmentally friendly, non-toxic or low-toxicity AF compounds and technologies (green AF agents) has become an urgent reality. Marine coatings based on Cu2O and various other biocides have a negative impact on the environment and they must eventually be replaced by new, effective, and environmentally friendly AF compounds. This mini-review describes important AF compounds discovered from a variety of organisms from 2004 until mid 2009, and discusses recent and general trends in the discovery of AF compounds. Finally, a perspective on the future of AF compound development is presented. The discussion is aimed at updating scientists and engineers on the current challenges facing AF research.

Description: The presented study assessed the heavy metal contamination risk in a former sludge deposit field of the River Ruhr in Essen, Germany. Therefore, the temporal and spatial distribution in soils and plants, chemical fractionation, mobilization potential, and transfer characteristics have been investigated. Soil samples, roots and shoots of rushes (Juncus sp.), and stem wood disks of willows (Salix sp.) were analyzed for Zn, Cu, Pb, Ni, Cr, and Cd. Plant available and mobile heavy metal portions have been determined using a sequential extraction procedure. The results show that the soils and the rushes are highly contaminated, although there is a considerable decrease compared to initial concentrations some 20 years ago. The willows show only small heavy metal enrichment. pH induced mobilization potential in soil is high for Cd, Zn and Ni. Additionally, these elements contain high portions of plant-available fractions. High transfer rates from soil to roots and very high rates from roots to shoots of rushes have been determined for Cd and Zn, indicating an accumulation of these elements in shoots of rushes. The rushes reflect the temporal and spatial heavy metal distribution in soil and might thus be used as a bioindicator or for phytoremediation.

Description: Improved monitoring of non-CO2 greenhouse gases in air samples is presented, achieved using a new analytical system based on preconcentration, gas-chromatography and mass spectrometry. In addition to the major HFCs, HCFCs and CFCs, the new observations include the first in situ time series of the C1–C3 PFCs (CF4, C2F6 and C3F8) and the more volatile of the HFCs (CHF3, CH2F2, CH3CF3) alongside SF6, all of which are now monitored routinely as part of the Advanced Global Atmospheric Gases Experiment (AGAGE). Observed trends in newly monitored species are shown, obtained from 1–2 years continuous in situ air analyses at remote monitoring sites at Mace Head (Ireland) and Cape Grim (Australia). Observed deviations in the air background for these gas species are linked to modelled trajectories of air masses arriving at the monitoring stations to indicate potential source regions for emissions in Europe and Australia. In addition, preliminary estimates of 2004 mixing ratio growth rates of compounds are deduced from the observations, which highlight the importance of continuous atmospheric monitoring for verification of consumption-based emission estimates of non-CO2 greenhouse gases.

Description: Development of microbial biofilms and the recruitment of propagules on the surfaces of man-made structures in the marine environment cause serious problems for the navies and for marine industries around the world. Current antifouling technology is based on the application of toxic substances that can be harmful to the natural environment. For this reason and the global ban of tributyl tin (TBT), there is a need for the development of “environmentally-friendly” antifoulants. Marine microbes are promising potential sources of non-toxic or less-toxic antifouling compounds as they can produce substances that inhibit not only the attachment and/or growth of microorganisms but also the settlement of invertebrate larvae and macroalgal spores. However, so far only few antilarval settlement compounds have been isolated and identified from bacteria. In this review knowledge about antifouling compounds produced by marine bacteria and diatoms are summarised and evaluated and future research directions are highlighted.

Description: The measurements of dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) in seawater are key in global change and coastal eutrophication studies. Nowadays, the high-temperature combustion (HTC) technique is a widely used method for DOC and TDN analysis. However, uncertainties exist about the operation of the catalyst in the conversion process of DOC and TDN in the HTC method. In this study, five different 'catalyst' materials were tested for their blanks, calibration slopes, and conversion efficiency of DOC and TDN using the Shimadzu TOC 5000A total organic carbon analyser coupled to a Sievers NCD 255 nitrogen chemiluminescence detector. The materials included four metallic catalysts (Shimadzu and Johnson 0.5 Pt-alumina, 13 Cu(II)O-alumina, 0.5 Pd-alumina) and quartz beads. The results indicated that DOC blank signals for the HTC approach using metallic catalysts with an alumina support are higher compared with quartz beads, as a result of the amphoteric nature of the alumina. However, the slopes of the standard calibration graphs were lowest for DOC and TDN determinations on the quartz beads. The DOC recoveries for the metallic catalysts were close to 100 for all compounds tested, with the exception of ammonium pyrrolidine dithiocarbamate. Using quartz beads, poor recoveries were obtained for a range of organic compounds, including the commonly used calibration compounds potassium hydrogen phthalate and glycine. The TDN recoveries for all compounds were typically >90, with the exception of NaNO2. Furthermore, analysis using the CuO-alumina and Pd-alumina catalysts and quartz beads showed low recoveries for NH4Cl. This study showed that catalyst performance should be verified on a regular basis using model compounds and blank checks made during every run, and that the Shimadzu 0.5 Pt-alumina material was an efficient catalyst for DOC and TDN analyses using the coupled total organic carbon-nitrogen chemiluminescence detector (TOC-NCD) analyser. © 2007 Taylor & Francis.

Description: Despite the importance of Ancistrocheirus lesueurii in the diet of a wide variety of oceanic predators, many aspects of its biology are unknown. We report new observations on the reproductive system of the species and provide age estimates of one normal and two intersexual males based on the number of increments in the statoliths. The age of the examined mature males was estimated to be more than 2 years, increasing the maximum age known for males of the species. Female A. lesueurii have specific modified areas for spermatangia reception in the nuchal region. The morphology of the right hectocotylized ventral arm and the relatively large spermatophore are also described.

Description: Draft regulations for prospecting and exploration of polymetallic sulphides in the “Area” are currently being considered for implementation by the International Seabed Authority. This paper examines possible models for allocation of lease blocks for exploration and the proposed schedule for relinquishing blocks during the exploration phase. One model for granting of exploration licenses under the proposed regulations involves contiguous lease blocks, similar to that being considered for nodules and crusts; another model involves non-contiguous blocks. An analysis of the occurrence and distribution of known polymetallic sulphides in 32 training areas shows that the regulations for prospecting and exploration of crusts likely cannot be applied equally to polymetallic sulphides.

Description: Data from two cross-shelf sediment sampling cruises were used to explain reasons for the sediment biogeochemical variability in respect of carbon, nitrogen and sulphur, and how the cycling of these elements governs the biogeochemistry of the overlying water through their control of the redox conditions. The spatial extent of this benthic–pelagic flux link is limited to the innershelf mud belt system on the Namibian shelf. The inshore mud belt is the primary deposition area of the carbon and nitrogen new production export flux. The offshore organic-rich zones are thought to be relict particulate organic matter originating from the inshore mud belt rather than from an overlying pelagic source. These data were used to set up a multi-layer sediment model that was used through sensitivity analyses to elucidate the input characteristics that result in the most significant feedbacks on hypoxia in the overlying water. The analyses showed that, although the new production flux is a requirement to drive an oxygen demand in the sediments, the onset and persistence of anoxia may depend critically on a low-oxygen boundary condition threshold. This is thought to be a key differentiating factor between systems that, despite comparable carbon export fluxes, are characterised by a persistent hypoxia/anoxia signal and those that are characterised by episodic hypoxia events. It was concluded that sediment oxygen demand and methane and 'sulphide' emissions from the central Benguela sediments are responses to external hypoxia boundary conditions rather than the local drivers of oxygen variability.

Description: Glyoxalase is one of two enzymes of the glyoxalase detoxification system against methylglyoxal and other aldehydes, the metabolites derived from glycolysis. The glyoxalase system is found almost in all living organisms: bacteria, protozoa, plants, and animals, including humans, and is related to the class of ‘life essential proteins’. The enzyme belongs to the expanded Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily. At present the GenBank contains about 700 of amino acid sequences of this enzyme type, and the Protein Data Bank includes dozens of spatial structures. We have offered a novel approach for structural identification of glyoxalase I protein family, which is based on the selecting of basic representative proteins with known structures. On this basis, six new subfamilies of these enzymes have been derived. Most populated subfamilies A1 and A2 were based on representative human Homo sapiens and bacterial Escherichia coli enzymes. We have found that the principle feature, which defines the subfamilies’ structural differences, is conditioned by arrangement of N- and C-domains inside the protein monomer. Finely, we have deduced the structural classification for the glyoxalase I and assigned about 460 protein sequences distributed among six new subfamilies. Structural similarities and specific differences of all the subfamilies have been presented. This approach can be used for structural identification of thousands of the so-called hypothetical proteins with the known PDB structures allowing to identify many of already existing atomic coordinate entrees.

Description: Morphological variability in gorgonians is frequent and commonly associated with habitat variability, often resulting in segregated morphotypes. Paramuricea macrospina is an endemic Mediterranean gorgonian species found on rocky bottoms between 40 and 160 m depth and has recently been reported as one of the most abundant species in continental shelf and shelf edge environments. Three different chromatic forms of P. macrospina were observed in the Menorca Channel: a yellow form and a light purple form occurring on maërl beds of the continental shelf, and a dark purple form occurring on rocky substrates of the shelf edge. The objective of the present work is to verify if these P. macrospina forms may represent distinct taxonomic units by analysing differences in colony morphology and sclerite size and shape of the three chromatic forms. No significant differences were found in colony shape, suggesting that environmental variability between the continental shelf and the shelf edge is not influential enough to significantly alter colony morphology. Significant differences in sclerite size and shape were found amongst all forms, suggesting that sclerites may be influenced by environmental conditions. However, the co-occurrence of the yellow and light purple forms side by side on the continental shelf may indicate a certain degree of genetic differentiation.

Description: The farming of the red seaweed Kappaphycus alvarezii and related species as raw material for the hydrocolloid carrageenan rapidly spread from the Philippines in the late 1960s to Indonesia, Tanzania, and other tropical countries around the world. Although numerous studies have documented positive socioeconomic impacts for seaweed farming, factors such as diseases and distance to export markets have led to an uneven development of the industry. Using standard budgeting techniques, this study adapted production and market data from a FAO-led global review of seaweed farming to develop comparative enterprise budgets for eight farming systems in six countries (Indonesia, the Philippines, Tanzania, India, Solomon Islands, and Mexico). Although the basic technology package is the same across countries, the study revealed large differences in the economic performance of systems due to wide variations in farm prices and the scale of operations. Although seaweed farming is a suitable activity for small-scale producers, a minimum of 2,000 m of cultures lines are still necessary to ensure adequate economic returns. Greater farming plots may be needed if farm prices are well below the average farm prices paid in Indonesia and the Philippines. Policy recommendations are made to improve the economic potential of underperforming systems.

Description: The feeding habits of two sympatric squid species, Uroteuthis (Photololigo) chinensis and Uroteuthis (Photololigo) duvaucelii from the southwestern Gulf of Thailand were studied. They fed on low numbers of food types (AF) and had a low diet breadth; 1.18 and 0.01 for U. (P.) chinensis and 1.49 and 0.05 for U. (P.) duvaucelii, respectively. Three major prey types (fishes, crustaceans and molluscs) were always detected and cannibalism was observed. Fish was the greatest contributor to the diet of both species, contributing 89.5% for U. (P.) chinensis and 69.9% for U. (P.) duvaucelii. Fish size significantly affected fullness index (FL) and AF for U. (P.) chinensis (P 〈 0.001) and U. (P.) duvaucelii (P 〈 0.001). Depth affected the FL of U. (P.) chinensis (P 〈 0.001) but not of U. (P.) duvaucelii (P 〉 0.05). Maturity stages of both male and female U. (P.) chinensis influenced FL (male: P 〈 0.001; female: P 〈 0.05) and AF (male: P 〈 0.05; female: P 〈 0.01). The FL of squid from cast nets was higher than those from trawls. The multivariate results showed dietary grouping between size classes of both species.

Description: Indian squid, Uroteuthis (Photololigo) duvaucelii (Loliginidae) constitute an important component of the inshore cephalopod fisheries along the eastern Arabian Sea. Local environmental variation plays an important role in species–environment interactions in neritic squids, which inhabit nearshore/coastal waters. Such ‘active’ and ‘passive’ responses of squids to environmental changes is crucial in understanding their relationships and influence on the biological processes, distribution and abundance of the fast-growing short-lived coastal loliginids. The empirical relationship between squid abundance and the variability in rainfall and sea surface temperature (SST) were explored in a tropical monsoon fishery. Monthly catch rates (catch per fishing hour) of squids in commercial trawl during 1987–2009 were used as the abundance index. Linear regression models with ARIMA errors were fitted with catch per unit hour time series as dependent variable and rainfall and SST as exogenous variables. While rainfall was observed to have a negative effect on squid abundance, the SST recorded a positive impact. ARIMA models provided satisfactory fit to observed data and forecast of 22 months. Given that the squid life-cycle is a function of their environment, this result is relevant in forecasting squid biomass for the management of tropical monsoon fisheries.

Description: The distribution pattern of purpleback flying squid and the relationship between its abundance and environmental variables in the south-eastern Arabian Sea were analysed using a geographical information system (GIS) and a generalised additive model (GAM). Highest abundances were observed during December and January within 10 and 13°N and 71 and 72°E around the Lakshadweep Islands. The mean abundance in the area was 4.21 tonnes/km2 and the maximum sustainable yield (MSY) was estimated as 0.63 million tonnes. GAM indicated that very high squid abundance was associated with low values of dissolved oxygen (6.01–6.27 mg/L), sea surface temperature (28.03–28.62°C), pH (7.75–8.56), salinity (30.42–34.48 psu), chlorophyll-a (0.4–0.6 mg/m3), zooplankton biomass (0.23–0.64 mL/m3), and shallow 20°C isotherm (101.2–134.7 m) and mixed layer depth (17.8–29.8 m). As a carnivore from paralarval stages, S. oualaniensis depends on secondary and tertiary production to sustain its population for which primary production is used up. In the tropical Arabian Sea where temperature differences are not so pronounced, S. oualaniensis distribution was found to be related to a low but very narrow sea surface temperature (SST) band. There is sufficient fishable biomass in the area to possibly launch a new targeted oceanic squid fishery from the western seaboard of India.

Description: Many studies have shown that natural marine compounds can prevent biofouling by a broad spectrum of organisms without toxic effects, encouraging their use in antifouling (AF) coatings. Studies over the past 25 years of the natural product zosteric acid (ZA) are systematically organized in this review. ZA is a sulfated phenolic acid produced by the seagrass Zostera marina that has very promising AF potential against several micro- and macrofouling organisms. ZA was shown to have appropriate environmental fate parameters such as high water solubility, a low log P, low bioaccumulation, and no ecotoxicity, which demonstrated the potential of ZA as a safe AF agent. This review also highlights that ZA has been successfully incorporated into several types of coatings. The synthesis of analogs is also considered in this review, and it has allowed a better understanding of ZA structure–AF activity relationships and clarified the mechanism of action of ZA.

Description: The environmental system of the northern Nordic Seas is very sensitive to oceanographic and climatic changes at the contact of cold Arctic and warmer North Atlantic waters. These contrasts are reflected in the associations of marine microorganisms and archived in the bottom sediments. A microfossil study (diatoms, coccoliths) of late Holocene sediments in core MSM5/5-712-1 from the eastern Fram Strait provides a better understanding of marine ecosystems and palaeoenvironments during Arctic warming events of the last two millennia. Indicative diatom species and groups of species revealed a high variability of sea-surface conditions. Based on the diatom distribution, three warming periods could be detected, corresponding to the time intervals of 0 to 440 CE (the later part of the Roman Warm Period), 1200 to1420 CE (the final part of the Medieval Climate Anomaly) and 1730 CE to present (including the Recent Warming). The various micropalaeontological proxies used in this study and other publications describe the Roman Warm Period and, especially, the Recent Warming as the most pronounced warm events in the area during the last 2000 years. A comparison of data from the different microfossil groups, indicators of sea-surface and subsurface conditions, reveals variable, complicated and non-simultaneous palaeoenvironmental signals within the warm periods. This can potentially be explained by changes in the surface/subsurface water structure during the events (variations in the cold/warm water advection, stratification, availability of nutrients, seasonal succession of bioproductivity, etc.), which are reflected by changes in the microplankton communities.

Description: Recent SST and atmospheric circulation anomaly data suggest that the 2015/16 El Niño event is quickly decaying. Some researchers have predicted a forthcoming La Niña event in late summer or early fall 2016. From the perspective of the modulation of tropical SST by solar activity, the authors studied the evolution of the 2015/16 El Niño event, which occurred right after the 2014 solar peak year. Based on statistical and composite analysis, a significant positive correlation was found between sunspot number index and El Niño Modoki index, with a lag of two years. A clear evolution of El Niño Modoki events was found within 1–3 years following each solar peak year during the past 126 years, suggesting that anomalously strong solar activity during solar peak periods favors the triggering of an El Niño Modoki event. The patterns of seasonal mean SST and wind anomalies since 2014 are more like a mixture of two types of El Niño (i.e. eastern Pacific El Niño and El Niño Modoki), which is similar to the pattern modulated by solar activity during the years following a solar peak. Therefore, the El Niño Modoki component in the 2015/16 El Niño event may be a consequence of solar activity, which probably will not decay as quickly as the eastern Pacific El Niño component. The positive SST anomaly will probably sustain in the central equatorial Pacific (around the dateline) and the northeastern Pacific along the coast of North America, with a low-intensity level, during the second half of 2016.

Description: The farming of the red seaweed Kappaphycus alvarezii and related species as raw material for the hydrocolloid carrageenan rapidly spread from the Philippines in the late 1960s to Indonesia, Tanzania, and other tropical countries around the world. Although numerous studies have documented positive socioeconomic impacts for seaweed farming, factors such as diseases and distance to export markets have led to an uneven development of the industry. Using standard budgeting techniques, this study adapted production and market data from a FAO-led global review of seaweed farming to develop comparative enterprise budgets for eight farming systems in six countries (Indonesia, the Philippines, Tanzania, India, Solomon Islands, and Mexico). Although the basic technology package is the same across countries, the study revealed large differences in the economic performance of systems due to wide variations in farm prices and the scale of operations. Although seaweed farming is a suitable activity for small-scale producers, a minimum of 2,000 m of cultures lines are still necessary to ensure adequate economic returns. Greater farming plots may be needed if farm prices are well below the average farm prices paid in Indonesia and the Philippines. Policy recommendations are made to improve the economic potential of underperforming systems.

Description: Geoengineering is regarded by advocates as a creative and responsible technological option in the face of a climate emergency. Critics often see it as a hubristic attempt to play God, with disastrous consequences for the planet and humanity. These antipodal perspectives are represented by the ideal types of Prometheans and Gaians. Prometheans and Gaians typically talk past each other. The geoengineering debate can be made more fruitful by well articulating their respective positions and subsequently situating them in the discourse of sustainability. A sustainability orientation does not answer the troubling question whether geoengineering should be developed and deployed. But it can foster a ‘fusion of horizons’ between Prometheans and Gaians, providing common ground in an otherwise polarized debate and making a more productive dialogue possible.

Description: Microplastic contamination of aquatic environments has become an increasingly alarming problem. These, defined as particles 〈5 mm, are mostly formed due to the cracking and embrittlement of larger plastic particles. Recent reports show that the increasing presence of microplastics in the environment could have significant deleterious consequences over the health of marine organisms, but also across the food chain. Herein, we have studied the effects of artificial seawater on polyethylene (PE)-based beads by exposing them up to eight weeks to saltwater in stirred batch reactors in the dark and examined the structural and morphological changes these endured. Electron microscopy observations showed that artificial seawater induces severe microcracking of the pellets' surfaces. Additionally, Fourier transform infrared spectroscopy (FTIR) analyses evidenced the formation of oxidized groups whenever these particles were exposed to water and an increase in organic matter content of the waters in which the pellets were kept was evidenced by Raman spectroscopy. There were also noticeable consequences in the thermal stability of the polyethylene pellets, as determined by thermogravimetric studies (TGA). Furthermore, the parallel exposure of polyethylene pellets to UV radiation yielded less pronounced effects, thus underscoring its lower preponderance in the degradation of this material. These results highlight the importance of determining the mechanisms of degradation of microplastics in marine settings and what the implications may be for the environment. Overall, the herein presented results show that a relatively short period of time of accelerated exposure can yield quantifiable chemical and physical impacts on the structural and morphological characteristics of PE pellets.

Description: Hydroacoustic and optical remote sensing have been commonly used to map shallow nearshore benthic features. However, the number, type, scale, and accuracy of the mapping products that can be obtained from the two sensors differ; as such, there can be limited agreement between their mapping products. These differences can be further accentuated if the hydroacoustic data are interpolated to produce a map. Interpolation introduces spatial uncertainty and reduces map accuracy. Consequently, maps generated from the two sensors may provide dissimilar spatial and temporal representations of the same benthic features. We therefore compared the performance of a random forest regression (RFr) and a universal kriging (UK) interpolation method and a post-classification enhancement that can be used to increase the accuracy and complementarity of benthic habitat maps derived from hydroacoustic data. First, we used single beam echosounder (SBES) survey bathymetry data from the Bluefields Bay marine protected area (MPA) in western Jamaica (13.82 km2 in size), to create a bathymetric surface model (BSM), from which rugosity and bathymetric position index (BPI) maps were generated. Next, the RFr was used to create submerged aquatic vegetation (SAV) percentage cover maps from the SBES SAV cover data by predicting cover at un-sampled locations. Predictors included auxiliary data such as depth, BPI, survey points coordinates and radiometrically corrected, deglinted and water column corrected image reflectance index values from each of the following: WorldView-2, Geoeye-1 and Landsat 8. Additionally, a SAV map was created using the UK. The most accurate SAV cover thresholds were identified and were used to create binary maps from the RFr and UK maps. A rugosity derived coral reef map was then added to the binary maps. The resulting benthic habitat maps had comparable accuracies and class coverage to benthic maps classified from GeoEye-1 and WorldView-2 images using pixel and object-based classifiers. However, map accuracies were calculated using a suboptimal number of reference points (〈50) for two of the benthic map classes (SAV absent and coral reef). This was not considered to be problematic as the addition of the coral reef class to the binary maps resulted in a significant decrease in uncertainty (standard error and confidence interval width of the overall accuracy) and a significant increase in the user’s accuracy of the SAV absent map class. Also, the difference in uncertainty and accuracy between the map classes did not change. The methods used in this study can therefore be used to increase the accuracy (and to decrease the uncertainty) and the complementarity of maps derived from hydroacoustic data.

Description: Purpose: To compare keratocyte activation, cellular morphologic changes and wound healing after SMILE and PRK procedures using transmission electron microscope (TEM).Methods: In this study, 22 New Zealand white rabbits (10- to 15-week old) were used. The right eyes of all animals underwent SMILE procedure and the left eyes underwent PRK procedure. Cornea samples taken 1 day and 1 week postoperatively were examined using TEM.Results: Using TEM 1 day after SMILE procedure, the organization of collagen fibers seemed to have been preserved without thermal alterations. Keratocyte activation was observed in the anterior stroma. Disrupted collagen arrangement and debris of cells are visible in the area of damage, and some phagocytic cells and a large number of secondary lysosomes are visible in those cells. At the perimeter zone of the interface, many coenocytes and collagen fragments could be found within the phagocytic cell. One week after SMILE procedure, potential lacuna could be discerned. A large part of the interface of the lenticule extracted had an appearance of clearly being adhered to some mucus secretions. One day after PRK procedure, an irregular epithelial surface was visible using TEM. Keratocytes had been activated and the rough endoplasmic reticulum in those cells had expanded. One week after PRK procedure, the epithelial surface still was irregular and keratinization of the epithelium was still visible in some areas. Corneal endothelium cells were mildly damaged and some vacuoles within the cytoplasm could be discerned. In the anterior stroma, some unhealthy activated keratocytes could still be observed. New collagen fibrils were found present near the activated keratocytes.Conclusion: Using TEM, keratocyte activation could still be observed after SMILE compared to after PRK procedure. Fewer cellular ultrastructural changes were seen after SMILE procedure. Unlike in PRK procedure, no damaged epithelium and endothelium were found after SMILE.

Description: Solar radiation is a forcing of the climate system with a quasi-11-year period. As a quasi-period forcing, the influence of the phase of the solar cycle on the ocean system is an interesting topic of study. In this paper, the authors investigate a particular feature, the ocean heat content (OHC) anomaly, in different phases of the total solar irradiance (TSI) cycle. The results show that almostopposite spatial patterns appear in the tropical Pacific during the ascending and declining phases of the TSI cycle. Further analysis reveals the presence of the quasi-decadal (~11-year) solar signal in the SST, OHC and surface zonal wind anomaly field over the tropical Pacific with a high level of statistical confidence (〉95%). It is noted that the maximum centers of the ocean temperature anomaly are trapped in the upper ocean above the main pycnocline, in which the variations of OHC are related closely with zonal wind and ocean currents.

Description: A series of triaxial compression tests of frozen sandy soil are carried out under five confining pressures (1 MPa, 4 MPa, 6 MPa, 8 MPa and 10 MPa) at –6 °C. By comparing the grain size distribution curves of frozen sandy soil before and after shearing, it is found that significant particle breakage can occur during triaxial shearing. Particle breakage changes internal structure of geomaterials and has a significant effect on their stress–strain relationships. In order to accurately describe the effect of particle breakage of frozen sandy soil on the stress–strain relationships, an elastoplastic constitutive model for frozen sandy soil considering particle breakage is proposed. Based on the energy balance equation established by Indraratna and Salim, the constant critical state stress ratio (Mcr) in the energy balance equation is modified to the stress ratio (M) which changed with shear strain during the shearing process. A yield function, considering particle breakage, is proposed using the modified energy balance equation. The hardening law is determined based on the rebound test results of frozen sandy soil, and the associated flow rule is adopted in the model. Compared with experimental data, the model can well simulate the stress–strain relationships under different confining pressures for frozen sandy soil. • Highlights • The particle breakage characteristics of frozen sandy soil are studied. • The energy balance equation considering particle breakage is modified to calculate the energy dissipation of frozen sandy soil. • An elastoplastic constitutive model for frozen sandy soil considering particle breakage is proposed.

Description: Surfactant-mediated extraction (SME), pressurized liquid extraction (PLE), and enzyme-assisted extraction (EAE) have been compared to improve the isolation of phlorotannins from the brown algae Lobophora variegata. Enzymatic treatment with Alcalase 2.4 L FG, Carezyme 4500 L, protease from Streptomyces griseus, pectinase from Aspergillus niger, Celluclast 1.5 L, protease from Bacillus licheniformis; surfactant extraction with triacetin and guaiacol and PLE with ethanol:water as extracting solvent, have been studied in terms of total phenolic content by the Folin–Ciocalteu method and total phlorotannin content using the DMBA assay. The results showed that SME yields the highest amount of phenols and phlorotannins by using food grade guaiacol as the surfactant. An extraction protocol was developed to maximize the amount of extract obtained from L. variegata. The effects of various parameters such as the type of surfactant, efficacy of surfactant, and optimum pH, on the extraction efficiency of polyphenols were examined. The simultaneous use of the enzyme and surfactant was also investigated. However, a synergistic effect between the enzymes and the surfactant for the extraction of polyphenols has not been observed. Considering total phenols and total phlorotannins in the extract, the extraction yield were obtained for total phenols as SME 〉 EAE 〉 PLE and for total phlorotannins as SME 〉 PLE 〉 EAE.