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  • Articles  (223)
  • Occupational Health and Environmental Toxicology  (223)
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  • Biology  (223)
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  • Physics  (223)
  • 101
    Electronic Resource
    Electronic Resource
    Comparison of bacterial growth to high-intensity microwave exposure and conventional heating (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 471-474 
    Details
    ISSN: 0197-8462
    Keywords: 8.8 GHz ; pulsed microwave ; thermal vs microwave alteration ; E coli ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Escherichia coli pol A+ and pol A- strains were exposed to 8.8-GHz microwaves pulsed at 1,000 Hz (1-μs pulse width) and an SAR of 40 W/kg, which increased the temperature of the cell culture by 7 °C. Two-way analysis of variance showed no significant difference between the growth rates of microwave irradiated and thermally exposed cells.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250030410
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  • 102
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    Masthead (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983) 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250040101
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  • 103
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    The influence of electric field exposure on bone growth and fracture repair in rats (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 11-19 
    Details
    ISSN: 0197-8462
    Keywords: electric field ; bone growth ; osteotomy repair ; rats ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Rats were exposed to a 60-Hz electric field at an unperturbed field strength of 100 kV/m to determine its affect on bone growth and fracture repair. Exposure of immature male and female rats for 20 h/day for 30 days did not alter growth rate, cortical bone area, or medullary cavity area of the tibia. In another experiment, midfibular osteotomies were performed and the juvenile rats were exposed at 100 kV/m for 14 days. Evaluation by resistance to deformation and breaking strength indicated that fracture repair was not as advanced in the exposed animals as in the shamexposed animals. In another experiment measurements of resistance to deformation were made in adult rats at 16, 20, and 26 days after osteotmy. Fracture repair was slower in exposed compared to control animals at day 20 and, to a lesser extent, at day 16, but not at day 26.
    Additional Material: 6 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250040103
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  • 104
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    Quantitative changes in potassium, sodium, and calcium in the submaxillary salivary gland and blood serum of rats exposed to 2880-MHz microwave radiation (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 55-62 
    Details
    ISSN: 0197-8462
    Keywords: 2880-MHz microwaves ; submaxillary salivary gland ; Na+ ; K+ ; Ca2+ ; flame photometry ; hyperthermia ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Na+, K+, and Ca2+ concentrations in the blood serum and submaxillary salivary gland (SSG) were investigated in adult, male rats exposed to 2880-MHz microwaves modulated with 1.5-μs pulses at a pulse repetition rate of 1000 Hz or in a hyperthermal environment. Rats were exposed, one at a time, for 30 min to microwaves producing a specific absorption rate (SAR) of: 4.2, 6.3,6.8,8.4, 10.8, or 12.6 W/kg, or were sham exposed under similar environmental conditions. In a second series, one group of rats was exposed singly for 15, 30, or 60 min to microwaves producing an SAR of 9.5 W/kg and other rats were exposed for similar periods at 40 °C; and 10 rats were sham exposed. Flame photometric analysis indicated that the thresholds of microwave radiation required to induce a change in Na+, K+, and Ca2+ concentrations in the salivary glands are 6.8, 6.8, and 6.3 W/kg, respectively. The directions of Na+, K+, and Ca2+ ion shifts in exposed rats' salivary glands are similar, whether affected by microwaves or hyperthermia. Greater changes in Na+ and K+ concentrations in SSG of rats exposed to microwaves for 15 and 30 min were found than in those exposed at 40 °C. On the other hand, exposure to hyperthermia at 40 °C or to microwaves for 1 h caused Na+ concentration to be increased by 68.7 and 59.5% and K+ concentration to be decreased by 29.6 and 21.7%, respectively.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250040106
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  • 105
    Electronic Resource
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    Chronic exposure of rabbits to 0.5 and 5 mW/cm2 2450-MHz CW Microwave Radiation (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 63-77 
    Details
    ISSN: 0197-8462
    Keywords: microwaves ; exposure chamber ; dosimetry ; rabbit ; body mass ; food consumption ; blood chemistry ; pathology ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Two groups of 16 male New Zealand rabbits were exposed to 2450-MHz continuous wave microwave fields in two experiments of 90 days each. The incident power densities of the first and second experiment were 0.5 and 5 mW/cm2, respectively. During each study, 16 animals were adapted to a miniature anechoic chamber exposure system for at least 2 weeks, then 8 of them were exposed for 7 h daily, 5 days a week for 13 weeks, and the other 8 animals were sham exposed. The rabbits were placed in acrylic cages, and each was exposed from the top in an individual miniature anechoic chamber. Thermography showed a maximum specific absorption rate of 5.5 W/kg in the head and 7 W/kg in the back at 5-mW/cm2 incident power density. After each 7-h exposure session, the animals were returned to their home cages. Food consumption in the exposure chamber and body mass were measured daily. Blood samples were taken before exposure and monthly thereafter for hematological, morphological, chemical, protein electrophoresis, and lymphocyte blast transformation studies. Eyes were examined for cataract formation. Finally, pathological examinations of 28 specimens of organs and tissues of each rabbit were performed. Statistically, there was a significant (P 〈 .01) decrease only of food consumption during the 5-mW/cm2 exposure; other variables were not significantly different between exposed and control groups.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250040107
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  • 106
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    Testicular function of rats following exposure to microwave radiation (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 107-114 
    Details
    ISSN: 0197-8462
    Keywords: spermatogenesis ; microwave radiation ; germinal tissue ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Male Sprague-Dawley rats were exposed for 6 h per day for nine days to pulse-modulated microwave radiation (1.3 GHz, at 1-μs pulse width, 600 pulses per second). Exposures were carried out in cylindrical waveguide sections at a mean dose rate of 6.3 mW/g; sham controls were treated similarly and received no irradiation. At time periods corresponding to 0.5, 1.0, 2.0, and 4.0 cycles of the seminiferous epithelium, groups of four shamirradiated and four irradiated rats were killed and the testes removed for analysis. Net mass of the testes, epididymides, and seminal vesicles; daily sperm production (DSP) per testis and per gram of testis; sperm morphology; and the number of epididymal sperm were determined. There were no statistically significant differences between the shamirradiated and irradiated groups with respect to any measured variable. In a group of seven surrogate animals of similar body mass, the dose rate of 6.3 mW/g caused a net change in body temperature (via rectal probe) of 1.5 °C.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250040202
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  • 107
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    Effect of microwave radiation on the beating rate of isolated frog hearts (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 5 (1984), S. 263-270 
    Details
    ISSN: 0197-8462
    Keywords: frog ; heart rate ; microwaves ; electrodes ; bradycardia ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: One hundred and two isolated frog hearts were divided into ten groups and placed individually in a waveguide filled with Ringer's solution and exposed to 2,450-MHz CW radiation at 2 and 8.55 W/kg. Heart rate was recorded using one of the following methods: 3-M KCl glass electrode, ultrasound probe, tension transducer, Ringer's solution glass electrode, and a metal wire inserted in the Ringer's solution electrode. An accelerated decrease of heart rate was observed only in those groups recorded using the 3-M KCl electrode and the metal wire Ringer's solution electrode. No effect was found in the other groups. These results indicate that bradycardia in isolated hearts could be caused by electrode artifacts resulting from the intensification of electromagnetic fields.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250050213
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  • 108
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    Hematologic and serum chemistry studies in rats exposed to 60-Hz electric fields (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 79-90 
    Details
    ISSN: 0197-8462
    Keywords: electric fields ; hematology ; serum chemistry ; rats ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Numerous hematologic and serum chemistry variables were examined in rats exposed to unperturbed 60-Hz electric fields at 100 kV/m for 15, 30, 60, or 120 days. Each study was replicated once. Rigorous statistical evaluations of these data did not detect any consistent effect of the electric field for exposures of up to 120 days. It was, however, not unusual in any individual study to detect certain variables that were significantly different between the exposed and shamexposed animals. This emphasizes the need for replicate designs and appropriate statistical analyses when investigating chemical or physical insults that may have minimal influence on biologic function.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250040108
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  • 109
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    Reproductive hazards among workers at high voltage substations (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 91-101 
    Details
    ISSN: 0197-8462
    Keywords: reproductive hazards ; congenital malformations ; high voltage ; epidemiological study ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A retrospective study on reproductive hazards was performed among 542 employees at Swedish power plants. Questionnaires were answered by 89% of the employees. Data on pregnancies were checked by studying hospital case records. There was a statistically significant, decreased frequency of „normal“ pregnancy outcome, almost exclusively due to an increased frequency of congenital malformations, when the father was a high-voltage switchyard worker. The differences in pregnancy outcome could not be explained by any of the confounding factors analyzed. The total number of children with malformations (26) and the total number of pregnancies in this study, however, were very small.
    Additional Material: 12 Tab.
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    URL: http://dx.doi.org/10.1002/bem.2250040109
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  • 110
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    Erratum (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 293-293 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250040309
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  • 111
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    Uniform DC electric field exposure systems for mice and cats (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 303-314 
    Details
    ISSN: 0197-8462
    Keywords: DC electric fields ; exposure systems ; finite difference method (FDM) ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In most previous 50/60-Hz experiments, subjects were placed in a dielectric cage and the electric field was applied from outside the cage. Although the field outside the cage was kept uniform in space and constant in time, the field inside the cage undergoes undesirable temporal and spatial variations. We have designed an electric-field exposure system that overcomes these problems by having a metal cage constitute a part of the field generating electrodes. The uniformity along the diameter of the cages for mice and cats are more than 84.2% and 74.3%, respectively.
    Additional Material: 10 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250040403
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  • 112
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    Perinatal exposure to 60-Hz electric fields: Effects on the development of the visual-evoked response in rats (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 327-339 
    Details
    ISSN: 0197-8462
    Keywords: 60-Hz electric fields ; perinatal exposure ; rat ; visual-evoked response ; central nervous system ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Two independent series of experiments were performed on 114 male Sprague-Dawley derived, albino rat pups, which represented 61 litters in experimental series I and 53 litters in experimental series II. Animals were exposed for 20 h/day from conception to testing (postnatal days 11-20) to a vertical, 65-kV/m, 60-Hz electric field or sham-exposed. Recordings of the visual-evoked response (VER) were obtained using a small silver ball electrode placed epidurally over the visual cortex. Visual stimuli consisted of 10-μS light flashes delivered at 0.2 Hz. Computer-averaged VERs were obtained and power spectral analyses (fast Fourier transform) were performed on the tapered (split cosine-bell window), averaged VERs. The expected age-related changes were clearly evident; however, a detailed analysis of VER component latencies, peak-to-peak amplitude, and power spectra failed to reveal any consistent, statistically significant effect of exposure to 60-Hz electric fields.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250040405
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  • 113
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    Microwaves (2,450 MHz) suppress murine natural killer cell activity (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 371-381 
    Details
    ISSN: 0197-8462
    Keywords: 2,450 MHz ; microwaves ; natural killer cells ; macrophages ; mice ; lymphocytes ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The effect of 2,450-MHz CW microwaves on natural killer (NK) cell activity and lymphocyte responsiveness to mitogen stimulation was studied in mice. Groups of mice were irradiated at power densities of 5, 15, or 30 mW/cm2 (SAR = 3.5, 10.5, and 21 W/kg respectively) for 1.5 h on 2 or 9 consecutive days. NK cell activity was determined using an in vitro 51Cr release cytotoxicity assay and an in vivo tumor-cell clearance assay. No consistent change was observed in the mitogen response of spleen cells from sham compared with irradiated mice. A significant suppression of NK cell activity measured in vitro was observed for mice irradiated at 30 mW/cm2, but not at 15 or 5 mW/cm2. A significant suppression of NK cell activity, as determined using the in vivo tumor clearance assay, was also observed at 30 mW/cm2. NK cell activity, as determined using the in vitro assay, returned to normal within 24 h following the last irradiation. Treatment of mice with hydrocortisone caused suppression of NK cell activity measured in vitro and in vivo. Paradoxically, peritoneal macrophage phagocytosis was enhanced following irradiation at 30 mW/cm2, the power density at which NK activity was suppressed. The possible role that microwave heating plays in producing these effects is discussed.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250040408
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  • 114
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    Hematologic and immunologic effects of pulsed microwaves in mice (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 383-396 
    Details
    ISSN: 0197-8462
    Keywords: hematology ; immunology ; mice ; pulsed microwaves ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Mice were exposed in the far field in an anechoic chamber to 2,880-MHz pulsed microwaves 3 to 7.5 h daily, 5 days/week for 60 to 360 h. Three experiments were performed at average power densities of 5 mW/cm2 and six at 10 mW/cm2, corresponding to averaged specific absorption rates (SARs) of 2.25 and 4.50 mW/g, respectively. Each experiment consisted of eight mice, with a concurrently sham-exposed group of eight. In two of three studies at 5 mW/cm2, there was a significant increase in bone marrow cellularity in the microwave-exposed groups compared to the sham-exposed groups. Significant differences were occasionally seen in erythrocyte, leukocyte, and platelet values from microwaveexposed groups, but were not consistently observed. In one of six groups exposed at 10 mW/cm2, mean bone marrow cellularity was reduced significantly in the microwaveexposed mice; in another group, the lymphocyte count was increased. In only one exposure (10 mW/cm2 for 360 h) was any significant effect noted on serum proteins: a reduction to 5.1 ± 0.3 g/dl in the exposed versus 5.6 ± 0.4 g/dl in the sham-exposed mice. This was due to a decrease in alpha and beta globulins, with no effect on albumin or gamma globulin concentrations. No effect on bone marrow granulocyte/macrophage colony-forming units (CFU) was revealed following exposure of mice to pulsed microwaves at 5 mW/cm2. In one of four exposures at 10 mW/cm2, there was a significant increase in CFU-agar colonies. No significant effects of exposures at 10 mW/cm2 were observed on in vivo and in vitro assays of cell-mediated immune functions. No exposure-related histopathologic lesions were found from examination of several tissues and organs. Results of these series of exposures of mice at SARs of 2.25 and 4.50 mW/g indicated no consistent effects on the hematologic, immunologic, or histopathologic variables examined.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250040409
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  • 115
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    In vitro effects of microwave radiation on rat liver mitochondria (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 5 (1984), S. 39-45 
    Details
    ISSN: 0197-8462
    Keywords: microwaves ; effects of mitochondria; nonionizing radiation ; mitochondrial respiration ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Liver mitochondria were exposed in vitro at 30°C to microwave radiation (2.45 GHz) during the following states of respiraton: resting, state 1; substrate dependent, state 2; ADP stimulated, state 3; and ADP depleted, state 4. At 10 or 100 mW/g, with succinate as substrate, no effect of exposure was observed on states 1-4 or the respiratory control index (state 3/state 4) of either tightly or loosely coupled mitochondria. When glutamate was used as substrate, no effects were observed at 10 mW/g. However, in the loosely coupled mitochondria the 100 mW/g exposure produced an increase in states 2 and 4 and a decrease in the respiratory control index. The results suggest that the function of loosely coupled mitochondria can be affected at high power levels of microwave radiation.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250050105
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  • 116
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    Microwave radiation-induced calcium ion efflux from human neuroblastoma cells in culture (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 5 (1984), S. 71-78 
    Details
    ISSN: 0197-8462
    Keywords: human neuroblastoma cells ; calcium ion efflux ; microwave ; amplitude modulation ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Monolayer cultures of human neuroblastoma cells were exposed to 915-MHz radiation, with or without sinusoidal amplitude modulation (80%) at 16 Hz, at specific absorption rates (SAR) for the culture medium and cells of 0.00, 0.01, 0.05, 0.075, 0.1, 0.5, 0.75, 1.0, 1.5, 2, or 5 mW/g. A significant increase in the efflux of calcium ions (45Ca2+) as compared to unexposed control cultures occurred at two SAR values: 0.05 and 1 mW/g. Increased efflux at 0.05 mW/g was dependent on the presence of amplitude modulation at 16 Hz but at the higher value it was not. These results indicate that human neuroblastoma cells are sensitive to extremely low levels of microwave radiation at certain narrow ranges of SAR.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250050108
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  • 117
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    Studies on prenatal and postnatal development in rats exposed to 60-Hz electric fields (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 5 (1984), S. 101-112 
    Details
    ISSN: 0197-8462
    Keywords: 60-Hz electric fields ; rats ; behavior ; teratology ; growth ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A series of three experiments was performed to determine the effects of 30-day exposures to uniform 60-Hz electric fields (100 kV/m) on reproduction and on growth and development in the fetuses and offspring of rats. In the first experiment, exposure of females for 6 days prior to and during the mating period did not affect their reproductive performance, and continued exposure through 20 days of gestation (dg) did not affect the viability, size, or morphology of their fetuses. In the second experiment, exposure of the pregnant rat was begun on 0 dg and continued until the resulting offspring reached 8 days of age. In the third experiment, exposure began at 17 dg and continued through 25 days of postnatal life. In the second and third experiments, no statistically significant differences suggesting impairment of the growth or survival of exposed offspring were detected. In the second experiment, a significantly greater percentage of the exposed offspring showed movement, standing, and grooming at 14 days of age than among-sham-exposed offspring. There was a significant decrease at 14 days in the percentage of exposed offspring displaying the righting reflex in the second experiment and negative geotropism in the third experiment. These differences were all transient and were not found when the animals were tested again at 21 days of age. Evaluation of the reproductive integrity of the offspring of the second experiment did not disclose any deficits.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250050111
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  • 118
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    Thin-layer liquid crystal thermometry of cells in vitro during hyperthermal microwave irradiation (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 237-245 
    Details
    ISSN: 0197-8462
    Keywords: liquid crystal thermometry ; microwave heating ; cells ; hyperthermia ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A nonperturbing technique of thin-layer liquid crystal thermometry was developed to quantitate heating of Chinese hamster ovary cells and the bacterium Serratia marcescens when exposed to 2450-MHz microwave fields at 0.2-0.5 W/cm2. Cells suspended in culture medium were injected into 5-cm glass microcapillary tubes coated on the inside with a thin layer of liquid crystal. The tubes were sealed and placed parallel to the electric field in a watertight waveguide exposure chamber where they were heated by circulating temperature-controlled water. Even at high circulation rates, liquid crystal color changes indicated local microwave capillary tube heating of 0.1-0.25 °C. Precision of measurement was 0.02 °C. Observations during microwave heating were significantly different from observations without microwaves at the 1% level, and heating increased as circulating water flow was reduced from 300 ml/s to 100 ml/s. The results of a cell survival assay following hyperthermal treatment were in good agreement with expectations based on the observations of microwave heating using liquid crystals.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250030208
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  • 119
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    Lack of effect of 2.45-GHz microwave radiation on the development of preimplantation embryos of mice (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 275-283 
    Details
    ISSN: 0197-8462
    Keywords: microwaves ; 2.45 GHz ; heat stress ; hyperthermia ; in vivo ; mouse ; preimplantation embryos ; development ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The development of preimplantation embryos after exposure to microwave radiation was studied. Female CD-1 mice were induced to superovulate, mated, and exposed to 2.45-GHz microwave or sham radiation for 3 h at power densities of 9 mW/cm2 and 19 mW/cm2 on either day 2 or 3 of pregnancy (plug day was considered day 1). Another group of mice was exposed to heat stress by placing the dams in an environmental room at an ambient temperature of 38 °C and relative humidity at 62% for 3 h on day 2 of pregnancy. All groups were euthanized on day 4 of pregnancy and embryos were recovered by flushing excised uterine horns. Embryos were examined for abnormalities and classified by the developmental stages. They were then treated with hypotonic solution and dissociated for counting blastomeres. Heat stress caused stunted development of embryos, but no remarkable effect of microwave radiation could be found on the development of preimplantation embryos.
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    URL: http://dx.doi.org/10.1002/bem.2250030211
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  • 120
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    Effects of weak amplitude-modulated microwave fields on calcium efflux from awake cat cerebral cortex (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 295-307 
    Details
    ISSN: 0197-8462
    Keywords: microwave fields ; calcium efflux ; cerebral cortex ; arousal ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Calcium (45Ca2+) efflux was studied from preloaded cortex in cats immobilized under local anesthesia, and exposed to a 3.0-mW/cm2 450-MHz field, sinusoidally amplitude modulated at 16 Hz (modulation depth 85%). Tissue dosimetry showed a field of 33 V/m in the interhemispheric fissure (rate of energy deposition 0.29 W/kg). Field exposure lasted 60 min. By comparison with controls, efflux curves from field exposed brains were disrupted by waves of increased 45Ca2+ efflux. These waves were irregular in amplitude and duration, but many exhibited periods of 20-30 min. They continued into the postexposure period. Binomial probability analysis indicates that the field-exposed efflux curves constitute a different population from controls at a confidence level of 0.96. In about 70% of cases, initiation of field exposure was followed by increased end-tidal CO2 excretion for about 5 min. However, hypercapnea induced by hypoventilation did not elicit increased 45Ca2+ efflux. Thus this increase with exposure does not appear to arise as a secondary effect of raised cerebral CO2 levels. Radioactivity measurements in cortical samples after superfusion showed 45Ca2+ penetration at about 1.7 mm/hr, consistent with diffusion of the ion in free solution.
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    Fluorescence depolarization studies of the phase transition in multilamellar phospholipid vesicles exposed to 1.0-GHz microwave radiation (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 323-332 
    Details
    ISSN: 0197-8462
    Keywords: diphenylhexatriene ; fluorescence depolarization ; microviscosity ; multilamellar phospholipid vesicles ; phase transition ; 1-GHz microwave radiation ; TEM cell ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The phase transition in multilamellar dimyristoylphosphatidylcholine (DMPC) vesicles was studied during exposure to continuous wave 1.0-GHz microwave radiation. Fluorescence depolarization measurements using a lipid-seeking molecular probe, diphenylhexatriene (DPH). were performed as a function of temperature. Semilog plots of microviscosity versus temperature illustrate the phase transition which shows a 5°C shift when the vesicles are treated with chloroform as a positive control. No shift of the phase transition was found during exposure to microwave radiation at specific absorption rates between 1 and 30 W/kg. Samples were exposed in a rectangular transmission line (TEM cell), and specific absorption rates were calculated from electrical measurements of incident, reflected, and transmitted power. Samples were exposed to increasing intensities of radiation, while the temperature was maintained at either 23.5 or 25.5 °C; these temperatures represented the two ends of the phase transition region for these vesicles. No statistically significant difference was found between exposed and control samples. These results are in contrast to those of others using laser Raman spectroscopy to measure the phase transition in similar multilamellar vesicles exposed to microwave radiation.
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    Local tumor hyperthermia using a computer-controlled microwave system (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 363-370 
    Details
    ISSN: 0197-8462
    Keywords: microwave ; hyperthermia ; hamster ; PARA-7 tumor ; computer ; 2.45 GHz ; temperature distribution ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A minicomputer-based system was designed to control the microwave (2.45-GHz) power to four local hyperthermia applicators. Errors in temperature measurement, due to electromagnetic field interactions with small thermocouple probes, are minimized by sampling the temperature only when the microwave power is off. The programmable controller can regulate the temperature in tumors in 0.1 °C increments from 30 to 60 °C. This technique reduces temperature differences throughout the tumor at steady state to less than 0.4 °C and prevents skin burns.
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    Masthead (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982) 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250030401
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    Measurement of blood-brain barrier permeation in rats during exposure to 2450-MHz microwaves (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 371-383 
    Details
    ISSN: 0197-8462
    Keywords: blood-brain barrier ; rats ; 2450-MHz microwaves ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Adult rats anesthesized with pentobarbital and injected intravenously with a mixture of [14C]sucrose and [3H]inulin were exposed for 30 min to an environment at an ambient temperature of 22, 30, or 40 °C, or were exposed at 22 °C to 2450-MHz CW microwave radiation at power densities of 0, 10, 20, or 30 mW/cm2. Following exposure, the brain was perfused and sectioned into eight regions, and the radioactivity in each region was counted. The data were analyzed by two methods. First, the data for each of the eight regions and for each of the two radioactive tracers were analyzed by regression analysis for a total of 16 analyses and Bonferroni's Inequality was applied to prevent false positive results from numerous analyses. By this conservative test, no statistically significant increase in permeation was found for either tracer in any brain region of rats exposed to microwaves. Second, a profile analysis was used to test for a general change in tracer uptake across all brain regions. Using this statistical method, a significant increase in permeation was found for sucrose but not for inulin. A correction factor was then derived from the warm-air experiments to correct for the increase in permeation of the brain associated with change in body temperature. This correction factor was applied to the data for the irradiated animals. After correcting the data for thermal effects of the microwave radiation, no significant increase in permeation was found.
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    Systems for exposing mice to 2,450-MHz electromagnetic fields (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 401-412 
    Details
    ISSN: 0197-8462
    Keywords: 2,450 MHz ; exposure system ; circular waveguide ; anechoic chamber ; mice ; dosimetry ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Two systems for exposing mice to 2,450-MHz electromagnetic fields are described. In a waveguide system, four mice were placed in a Styrofoam cage and exposed dorsally to circularly polarized electromagnetic fields. The temperature and humidity in the mouse holder were kept constant by forced-air ventilation. For 1-W input power to the waveguide, the average specific absorption rate (SAR) was determined by twin-well calorimetry to be 3.60 ± 0.11 (SE) W/kg in 27-g mice. The maximum SAR at the skin surface determined thermographically was 8.36 W/kg in the head of the mouse. The second system was a miniature anechoic chamber. Six mice were irradiated dorsally to far field plane waves. Copper shielding and high-temperature absorbing material were lined inside the chamber to accommodate the high input power. The air ventilation at the location of the mice was separately controlled so that any heating in the absorber would not affect the animals. For 1-W input power, the average SAR was 0.17 ± 0.01 W/kg and the maximum SAR at the skin surface was 0.41 W/kg in the animal when irradiated with body axis parallel to the E field; the SARs were 0.11 ± 0.01 W/kg and 0.64 W/kg, respectively, when irradiated perpendicular to the E field.
    Additional Material: 9 Ill.
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    A behavioral response of swine to a 60-Hz electric field (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 443-451 
    Details
    ISSN: 0197-8462
    Keywords: miniature swine ; ELF ; 60-Hz electric field ; behavior ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: It has been shown that rats, given the choice, will spend more time out of a 60-Hz electric field than in it at field strengths ≥ 75 kV/m. This paper describes research to examine the relevance of these data to a different species, the pig. Miniature pigs that had been exposed to a 60-Hz electric field at 30 kV/m for 20 h/day, 7 days/week for as long as 6 months, were tested for their preference for the presence or absence of the field during a 23.5-h period. Similar to earlier results with rats, miniature pigs spent more time out of the electric field than in it during the sleeping period.
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    Electromagnetic Fields Induced by Helmholtz Aiding Coils Inside Saline-Filled Boundaries (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 357-370 
    Details
    ISSN: 0197-8462
    Keywords: Helmholtz coils ; electromagnetic fields ; induced currents ; saline media ; biological tissue ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The use of Helmholtz aiding coils to induce small electromagnetic (EM) fields in living tissue for both medical and research purposes has become quite common. While much progress has been made in showing that these induced EM signals can cause a variety of effects in tissues and individual cells, a satisfactory explanation of how the effects occur or how the EM signals couple to the tissue has not yet emerged. To address the latter problem adequately, it becomes necessary to know the spatial distribution of the induced fields inside a given set of boundaries. This paper examines the situation used for much in vitro research where a cylindrical culture dish is filled with a conducting solution and placed between the Helmholtz coils. Two cases are considered. The first assumes that the coils are above and below the culture dish (the planes of the coils are parallel with the top and bottom of the dish); the second assumes that the planes of the coils are parallel with the sides of the dish. A closed form solution is obtained for both cases, and it is shown that the induced EM field distribution is markedly different for the two cases.
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    Acoustical imaging of a model of a human hand using pulsed microwave irradiation (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 397-400 
    Details
    ISSN: 0197-8462
    Keywords: acoustical imaging ; microwave-induced acoustics ; pulsed microwaves ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Pulsed 5.66-GHz microwave energy irradiated a model of a human hand that was positioned above a submerged planar array of 400 hydrophones. Hydrophone response data were analyzed by a computer that graphically reproduced the image.
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    Magnetic field effects on calcium efflux and insulin secretion in isolated rabbit islets of Langerhans (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 103-106 
    Details
    ISSN: 0197-8462
    Keywords: Calcium efflux ; insulin ; secretion ; islets of Langerhans ; rabbit ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Rabbit islets of Langerhans were exposed at 37 °C for 18 h to a low-frequency-pulsed magnetic field, generated in paired Helmholtz coils. Exposed islets showed a reduction of 26.1 ± 4.3% in 45Ca2+ content (P 〈 .004). a reduction of 25.1 ± 6.3% in 45Ca2+ efflux (P 〈 .006), and a reduction of 35.0 ± 8.7% (P 〈 .002) in insulin released during glucose stimulation when compared with appropriate controls.
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    Effects of microwave exposure on the hamster immune system. I. Natural killer cell activity (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 123-139 
    Details
    ISSN: 0197-8462
    Keywords: microwave immunosuppression ; natural killer cells ; hamster immune system ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Hamsters were exposed to repeated or single doses of microwave energy and monitored for changes in core body temperature. circulating leukocyte profiles, serum corticosteroid levels, and natural killer (NK) cell activity in various tissues. NK cytotoxicity was measured in a 15Crrelease assay employing baby hamster kidney (BHK) targets or BHK infected with herpes simplex virus. Repeated exposure of hamsters at 15 mW/cm2 for 60 min/day had no significant effect on natural levels of spleen-cell NK activity against BHK targets. Similarly, repeated exposure at 15 mW/cm2 over a 5-day period had no demonstrable effect on the induction of spleen NK activity by vaccinia virus immunization, that is. comparable levels of NK were induced in untreated and microwave-treated animals. In contrast, treatment of hamsters with a single 60-min microwave exposure at 25 mW/cm2 caused a significant suppression in induced spleen NK activity. A similar but less marked decrease in spleen NK activity was observed in sham-exposed animals. Moreover, the sham effects on NK activity were not predictable and appeared to represent large individual animal variations in the response to stress factors. Depressed spleen NK activity was evident as early as 4 h postmicrowave treatment and returned to normal levels by 8 h. Hamsters exposed at 25 mW/cm2 showed an elevated temperature of 3.0-3.5 °C that returned to normal within 60 min after termination of microwave exposure. These animals also showed a marked lymphopenia and neutrophilia by 1 h posttreatment that returned to normal by 8-10 h. Serum glucocorticosteroids were elevated between 1 and 8 h after microwave treatment. Sham-exposed animals did not demonstrate significant changes in core body temperature. peripheral blood leukocyte (PBL) profile, or glucocorticosteroid levels as compared to minimum-handling controls.
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    Relationship between field strength and arousal response in mice exposed to 60-Hz electric fields (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 181-191 
    Details
    ISSN: 0197-8462
    Keywords: 60-Hz electric fields ; arousal response ; electric field strength ; mice ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: White-footed mice, Peromyscus leucopus, were exposed to 60-Hz electric fields to study the relationship between field strength and three measures of the transient arousal response previously reported to occur with exposures at 100 kV/m. Five groups of 12 mice each were given a series of four 1-h exposures, separated by an hour, with each group exposed at one of the following field strengths: 75, 50, 35, 25, and 10 kV/m; 8 additional mice were sham-exposed with no voltage applied to the field generator. All mice were experimentally naive before the start of the experiment, and all exposures occurred during the inactive (lights-on) phase of the circadian cycle. The first exposure produced immediate increases in arousal measures, but subsequent exposures had no significant effect on any measure. These arousal responses were defined by significant increases of gross motor activity, carbon dioxide production, and oxygen consumption, and were frequently recorded with field strengths of 50 kV/m or higher. Significant arousal responses rarely occurred with exposures at lower field strengths. Responses of mice exposed at 75 and 50 kV/m were similar to previously described transient arousal responses in mice exposed to 100-kV/m electric fields. Less than half of the mice in each of the field strength groups below 50 kV/m showed arousal responses based on Z (standard) scores, but the arousals of the mice that did respond were similar to those of mice exposed at higher field strengths. Polynomial regression was used to calculate the field strength producing the greatest increases for each of the arousal measures. The results show that the amplitude of the transient arousal response is related to the strength of the electric field, but different measures of arousal may have different relationships to field strength.
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    Psychoactive-drug response is affected by acute low-level microwave irradiation (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 205-214 
    Details
    ISSN: 0197-8462
    Keywords: microwaves ; apomorphine ; amphetamine ; morphine ; behavior ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The effects of various psychoactive drugs were studied in rats exposed for 45 min in a circularly polarized, pulsed microwave field (2450 MHz; SAR 0.6 W/kg; 2-μs pulses, 500 pps). Apomorphine-induced hypothermia and stereotypy were enhanced by irradiation. Amphetamine-induced hyperthermia was attenuated while stereotypy was unaffected. Morphine-induced catalepsy and lethality were enhanced by irradiation at certain dosages of the drug. Since these drugs have different modes of action on central neural mechanisms and the effects of microwaves depend on the particular drug studied, these results show the complex nature of the effect of microwave irradiation on brain functions.
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    Suppression of T-lymphocyte cytotoxicity following exposure to sinusoidally amplitude-modulated fields (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 281-292 
    Details
    ISSN: 0197-8462
    Keywords: microwaves ; amplitude modulation ; murine allogeneic cytotoxicity ; T lymphocytes ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Significant inhibition of allogeneic cytotoxicity of the target cell MPC-11 by the murine cytotoxic T-lymphocyte line CTLL-1 was observed when the 4-h cytotoxicity assay was conducted in the presence of a 450-MHz field sinusoidally amplitude-modulated at 60 Hz. Exposure of the effector cells to the field prior to adding them to the target cells in the cytolytic assay resulted in a similar inhibition, suggesting a direct interaction of the field with the cytolytic T lymphocyte. The inhibition was preferentially expressed during the early allogeneic recognition phase. Fieldexposed cytolytic cells recovered their full cytolytic capacity in 12.5 h. A differential susceptibility was observed with modulation frequencies from 0 to 100 Hz. Peak suppression occurred at 60 Hz modulation, with progressively smaller effects at 40, 16, and 3 Hz. The unmodulated carrier wave did not affect the cytotoxicity. Effects with 80- and 100-Hz modulation were smaller than at 60 Hz. These results demonstrate an inhibitory but recoverable effect by certain amplitude modulations of weak nonionizing radiation upon the cell-mediated cytolytic immune response.
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    Masthead (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980) 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250010101
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    Microwaves modify thermoregulatory behavior in squirrel monkey (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 1-20 
    Details
    ISSN: 0197-8462
    Keywords: 2,450 MHz CW microwaves ; behavioral thermoregulation ; body temperature ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Squirrel monkeys (Saimiri sciureus) trained to regulate environmental temperature (Ta) behaviorally were exposed in the far field of a horn antenna to ten-minute periods of 2,450 MHz CW microwaves. Incident power density ranged from 1 to 22 mW/cm2. The corresponding specific absorption rate (SAR), derived from temperature increments in saline-filled styrofoam models, ranged from 0.15 to 3.25 W/kg. Controls included exposure to infrared radiation of equivalent incident energy and no radiation exposure. Normal thermoregulatory behavior produces tight control over environmental and body temperatures; most monkeys select a Ta of 34-36°C. Ten-minute exposures to 2,450 MHz CW microwaves at an incident power density of 6-8 mW/cm2 stimulated all animals to select a lower Ta. This threshold energy represents a whole-body SAR of 1.1 W/kg, about 20% of the resting metabolic rate of the monkey. Thermoregulatory behavior was highly efficient, and skin and rectal temperatures remained stable, even at 22 mW/cm2 where the preferred Ta was lowered by as much as 4°C. No comparable reduction in selected Ta below control levels occurred during exposure to infrared radiation of equal incident power density.
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    Microwave-induced pressure waves in a model of muscle tissue (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 45-54 
    Details
    ISSN: 0197-8462
    Keywords: pulsed microwaves ; thermoelastic waves ; simulated tissue ; hydrophone ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Microwave-induced mechanical stress waves were studied in simulated muscle tissue. Pulsed microwave energy at 5.655 GHz induced pressure waves that were recorded with a hydrophone transducer. Each pulse produced a peak power density greater than 1.5 kW/cm2. Microwave absorption measurements within the model showed energy deposition to be mostly confined to a region within 2 cm of the irradiated surface. The average specific absorption rate (SAR) at the surface of the sample was about 100 W/kg. The microwave-induced stress wave propagated at a velocity of 1,600 m/sec with peak pressures of approximately 300 pascals and was detectable after having traveled a total distance of 0.61 m on a path that included two reflections at model-container interfaces.
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    The differential effects of 200, 591, and 2,450 MHz radiation on rat brain energy metabolism (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 5 (1984), S. 419-433 
    Details
    ISSN: 0197-8462
    Keywords: radiofrequency ; brain ; metabolism ; stripline ; fluorescence ; mechanism ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Three key compounds in brain energy metabolism have been measured during and after exposure to continuous wave radiofrequency radiation at 200, 591, and 2,450 MHz. Frequency-dependent changes have been found for all three compounds. Changes in NADH fluorescence have been measured on the surface of a surgically uncovered rat brain during exposure. At 200 and 591 MHz, NADH fluorescence increased in a dose-dependent manner between approximately 1 and 10 mW/cm2, then became constant at higher exposures. There was no effect at 2,450 MHz. Levels of ATP and CP were measured in whole brain after exposure. The ATP levels were decreased at 200 and 591 MHz but not at 2,450 MHz. The CP levels decreased only at 591 MHz. The effect of duration of exposure (up to 5 min) was investigated for all compounds at 200 MHz and 2,450 MHz, and exposures to 20 minutes were examined at 591 MHz. Temperature in the rat brain was essentially constant for all exposures. A general mechanism for inhibition of the mitochondrial electron transport chain and the CP-kinase reaction pathway by radiofrequency radiation has been proposed.
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    Comparison of the coupling of grounded humans, swine and rats to vertical, 60-Hz electric fields (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 117-129 
    Details
    ISSN: 0197-8462
    Keywords: electric fields ; 60 Hz ; biological effects ; dosimetry ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Published and new data for grounded humans, swine, and rats exposed to vertical, 60-Hz electric fields are used to determine field strengths at the surfaces of the bodies and average components of induced-current density along the axes of the bodies. At the tops of the bodies, surface electric fields are increased (enhanced) over the unperturbed field strength present before the subjects entered the field by factors of 17,7, and 4 for humans, swine, and rats, respectively. For an unperturbed field strength of 10 kV/m, average induced axial current densities in the neck, chest, abdomen, and feet are: 550, 190, 250, and 2000 nA/cm2, respectively, for humans; 40, 13, 20, and 1100 nA/cm2, respectively, for swine; and 28, 16, 2, and 1400 nA/cm2, respectively, for rats. These data are used to show that the actual electric fields experienced by animals depend strongly on the shape of the body and its orientation relative to the electric field and ground plane. This fact must be taken into account if biological data obtained with laboratory animals are to be used for the assessment of possible hazards to humans exposed to 60-Hz electric fields.
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    Far-field microwave dosimetry in a rhesus monkey model (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 149-160 
    Details
    ISSN: 0197-8462
    Keywords: microwaves ; microwave dosimetry ; rhesus monkey dosimetry ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Dosimetric measurements were made in a muscle-equivalent model of an adult rhesus monkey subjected to far-field irradiation at 1.29 GHz. Profiles of microwave-induced heating in the model were obtained at eight locations, and a gradient-layer whole-body calorimeter was used to measure total absorbed energy. Average specific absorption rate (SAR) was calculated both from the calorimeter experiments and from the local temperature measurements. Thermographic imaging techniques were used to qualitatively show the microwave-induced surface heating patterns. For this model the calculated average SAR was 0.155 (W/kg)/(mW/cm2) which, at 1.29 GHs, makes the absorption cross section 84% of the geometric shadow cross section. The SAR is about three times that predicted for a prolate spheroidal model of similar mass. A disproportionally high absorption occured in the legs of the model positioned parallel to the E-polarization because of what is believed to be partial-body resonance.
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    Chronic exposure to a 60-Hz electric field: Effects on synaptic transmission and peripheral nerve function in the rat (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 131-147 
    Details
    ISSN: 0197-8462
    Keywords: electric fields ; rat ; sciatic nerve ; vagus nerve ; superior cervical sympathetic ganglion ; chronic exposure ; 60 Hz ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Several reports have suggested that the nervous system can be affected by exposure to electric fields and that these effects may have detrimental health consequences for the exposed organism. The purpose of this study was to investigate the effects of chronic (30-day) exposure of rats to a 60-Hz, 100-kV/m electric field on synaptic transmission and peripheral-nerve function. One hundred forty-four rats, housed in individual polycarbonate cages were exposed to uniform, vertical, 60-Hz electric fields in a system free of corona discharge and ozone formation and in which the animals did not receive spark discharges or other shocks during exposure. Following 30 days of exposure to the electric field, superior cervical sympathetic ganglia, vagus and sciatic nerves were removed from rats anesthetized with urethan, placed in a temperature-controlled chamber, and superfused with a modified mammalian Ringer's solution equilibrated with 95% O2 and 5% CO2. Several measures and tests were used to characterize synaptic transmission and peripheral-nerve function. These included amplitude, area, and configuration of the postsynaptic or whole-nerve compound-action potential; conduction velocity; accommodation; refractory period; strength-duration curves; conditioning-test (C-T) response, frequency response; post-tetanic response; and high-frequency-induced fatigue. The results of a series of neurophysiologic tests and measurements indicate that only synaptic transmission is significantly and consistently affected by chronic (30-day) exposure to a 60-Hz, 100-kV/m electric field. Specifically, an increase in synaptic excitability was detected in replicated measurements of the C-T response ratio. In addition, there are trends in other data that can be interpreted to suggest a generalized increase in neuronal excitability in exposed animals.
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    The Bioelectromagnetics Society abstracts of Papers to be presented at second annual meeting, September 14-18, 1980. (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 199-251 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250010209
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    Effect of microwaves (2450-MHz) on the immune system in mice: Studies of nucleic acid and protein synthesis (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 161-170 
    Details
    ISSN: 0197-8462
    Keywords: microwave bioeffects ; nonionizing radiation ; lymphoid cell metabolism ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: CBA/J adult male mice were given single or triple exposures to 2450-MHz microwaves in an environmentally controlled wave guide facility. The average absorbed dose rate for a single exposure varied from 12 to 15 mW/g. Shamexposed mice served as controls. Lymphoid cells were collected and tested for metabolic activity on days 3, 6, and 9 following a single exposure, and on days 9, 12, and 16 following triple exposures on days 0, 3, and 6. Cells were cultured in vitro for four hours to seven days before their metabolic rates were assayed. Under these conditions, microwaves failed to produce any detectable change in deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein synthesis, as measured by the incorporation of methyl(3H)-thymidine (3H-TDR) (DNA substrate), 3H-uridine (3H-UR) (RNA substrate), and 3H-leucine (protein substrate) by spleen, bone marrow, and peripheral blood lymphocytes (PBL) in vitro. These data suggest that microwave-induced increases in the frequency of complement-receptor (CR)- or surface-immunoglobulin (sIg)-bearing cells were not associated with a concomitant increase in cell proliferation and/or protein synthesis, and favor the concept that microwaves under these conditions stimulate already existing B-cell precursors for maturation.
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    Observing-responses of rats exposed to 1.28- and 5.62-GHz microwaves (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 183-198 
    Details
    ISSN: 0197-8462
    Keywords: operant behavior ; observing-responses ; microwaves ; vigilance ; dosimetry ; rats ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The effects of microwave irradiation at two different frequencies (1.28 and 5.62 GHz) on observing-behavior of rodents were investigated. During daily irradiation, eight male hooded rats performed on a two-lever task; depression of one lever produced one of two different tones and the other lever produced food when depressed in the presence of the appropriate tone. At 5.62 GHz, the observing-response rate was not consistently affected until the power density approximated 26 mW/cm2 at 1.28 GHz, the observing-response rate of all rats was consistently affected at a power density of 15 mW/cm2. The respective whole-body specific absorption rates (SARs) were 4.94 and 3.75 W/Kg. Measurements of localized SAR in a rat-shaped model of simulated muscle tissue revealed marked differences in the absorption pattern between the two frequencies. The localized SAR in the model's head at 1.28 GHz was higher on the side distal to the source of radiation. At 5.62 GHz the localized SAR in the head was higher on the proximal side. It is concluded that the rat's observing behavior is disrupted at a lower power density at 1.28 than at 5.62 GHz because of deeper penetration of energy at the lower frequency, and because of frequency-dependent differences in anatomic distribution of the absorbed microwave energy.
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    Microwave effects on energy metabolism of rat brain (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 171-181 
    Details
    ISSN: 0197-8462
    Keywords: microwaves ; in vivo ; brain ; adenosine triphosphate (ATP) ; creatine phosphate (CP) ; NADH ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Rat brain was exposed to 591-MHz, continuous-wave (CW) microwaves at 13.8 or 5.0 mW/cm2 to determine the effect on nicotinamide adenine dinucleotide, reduced (NADH), adenosine triphosphate (ATP) and creatine phosphate (CP) levels. On initiation of the in vivo microwave exposures, fluorimetrically determined NADH rapidly increased to a maximum of 4.0%-12.5% above pre-exposure control levels at one-half minute, then decreased slowly to 2% above control at three minutes, finally increasing slowly to 5% above control level at five minutes. ATP and CP assays were performed on sham- and microwave-exposed brain at each exposure time. At 13.8 mW/cm2, brain CP level was decreased an average of 39.4%, 41.1%, 18.2%, 13.1%, and 36.4% of control at exposure points one-half, one, two three, and five minutes, respectively, and brain ATP concentration was decreased an average of 25.2%, 15.2%, 17.8%, 7.4%, and 11.2% of control at the corresponding exposure periods. ATP and CP levels of rat brain exposed to 591-MHz cw microwaves at 5 mW/cm2 for one-half and one minute were decreased significantly below control levels at these exposure times, but were not significantly different from the 13.8 mW/cm2 exposures. For all exposures, rectal temperature remained constant. Heat loss through the skull aperture caused brain temperature to decrease during the five-minute exposures. This decrease was the same in magnitude for experimental and control subjects. Changes in NADH, ATP, and CP levels during microwave exposure cannot be attributed to general tissue hyperthermia. The data support the hypothesis that microwave exposure inhibits mitochondrial electron transport chain function, which results in decreased ATP and CP levels in brain.
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    Masthead (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980) 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250010301
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    A thermal model of the human body exposed to an electromagnetic field (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 253-270 
    Details
    ISSN: 0197-8462
    Keywords: thermal model ; heat transfer ; dosimetry, 80 MHz ; 200 MHz ; absorption of electromagnetic energy ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The human body was modeled by numerical procedures to determine the thermal response under varied electromagnetic (EM) exposures. The basic approach taken was to modify the heat transfer equations for man in air to account for thermal loading due to the energy absorbed from the EM field. The human body was represented in an EM model by a large number of small cubical cells of tissue, and the energy density was determined for each cell. This information was then analyzed by a thermal response model consisting of a series of two-dimensional transient conduction equations with internal heat generation due to metabolism, internal convective heat transfer due to blood flow, external interaction by convection and radiation, and cooling of the skin by sweating and evaporation. This model simulated the human body by a series of cylindrical segments. The local temperature at 61 discrete locations as well as the thermoregulatory responses of vasodilatation and sweating were computed for a number of EM field intensities and two frequencies, one near whole-body resonance.
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    Power density, field intensity, and carrier frequency determinants of RF-energy-lnduced calcium-ion efflux from brain tissue (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 271-275 
    Details
    ISSN: 0197-8462
    Keywords: calcium ions ; brain tissue ; radiofrequency (RF) radiation ; complex permittivity (∊r* ; 50 MHz ; 147 MHz ; 450 MHz ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: To explain a carrier frequency dependence reported for radiofrequency (RF)-induced calcium-ion efflux from brain tissue, a chick-brain hemisphere bathed in buffer solution is modeled as a sphere within the uniform field of the incident electromagnetic wave. Calculations on a spherical model show that the average electric-field intensity within the sample remains the same at different carrier frequencies if the incident power density (Pi) is adjusted by an amount that compensates for the change in complex permittivity (∊r*) and the change of wavelength, as a function of carrier frequency. The resulting formula for transforming Pi is seen to follow the pattern of both positive and negative demonstrations of calcium-ion efflux that have been observed at carrier frequencies of 50, 147, and 450 MHz. Indeed, all results obtained at these three frequencies, when related by Pi's that produce the same average electric-field intensity within the sample, are seen to be in agreement; no prediction is contradicted by an experiment.
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    Effects of 60-Hz electric fields on avoidance behavior and activity of rats (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 299-312 
    Details
    ISSN: 0197-8462
    Keywords: ELF electric field ; 60-Hz ; avoidance behavior ; activity ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In repeated short-term tests (four sessions, each of 45-minute duration), and one longer test (a 23.5-hour session), behavior of rats was evaluated in a long, narrow shuttlebox. One side of the box was exposed to an electric field at various strengths, while a visually identical opposite side was shielded from exposure. In the short-term tests, rats generally remained shielded from electric fields of 90 kV/m and greater during the first session, and maintained this response in subsequent sessions. In the longer test, this same preference response was demonstrated at field strengths of 75 kV/m and greater; however, at 25 and 50 kV/m, rats exhibited a statistically significant preference for the exposed region of the shuttlebox, but only during the light portion of a 12-hour light: 12-hour dark cycle. Exposed animals made more traverses than sham-exposed controls between the two ends of the shuttlebox during the first hour of the test. The experimental data support the hypothesis that the observed behavioral effects are the result of direct interaction of the electric field with the animal, and not the result of secondary factors such as electric shock, corona discharge, audible noise, ozone, or vibration of the experimental apparatus.
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    A computer model of temperature distribution inside a lossy sphere after microwave radiation (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 337-343 
    Details
    ISSN: 0197-8462
    Keywords: computer model ; temperature distribution ; lossy sphere ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The temperature distribution inside a lossy sphere resulting from the absorption of microwave energy was approximated by successive numerical iterations, of the thermal energy equation. Heat transfer within the sphere by conduction was considered. In the model energy was not dissipated by convection but was contained in the sphere for over 200 seconds. Exposure of a 5-cm sphere to 3,000 MHz at 30 mW/cm2 for 200 seconds was calculated to produce a temperature rise of 0.56°C near the front surface.
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    Dosimetry for a study of effects of 2.45-GHz microwaves on mouse testis (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 325-336 
    Details
    ISSN: 0197-8462
    Keywords: microwave ; dosimetry ; mouse testis ; 2.45 GHz ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In order to determine the effects of microwave radiation on the testis, it is necessary to express the physical insult in animal studies in a way that can be replicated elsewhere and ultimately used as a basis for extrapolation to man. However, there is conflict  -  especially in chronic experiments  -  between the desire for precise dosimetry and the need to minimise alteration of the normal physiological functions of the animals. The compromise arrangement used in this study was to house the mice singly, in cages with limited food and water, and to irradiate them for up to 30 days (16 h/day) in an anechoic chamber. The only measurements taken routinely were of power density in the positions normally occupied by the cages. In addition, a series of absorption measurements was made in mouse carcasses: Whole-body specific absorption rate (SAR); energy-deposition patterns (determined thermographically); and local SAR in testis (using a miniature electric (E)-field probe). It was concluded that the SAR in testis was considerably less than the whole-body SAR. Exposure for 16 h at 50 mW/cm2 elevated rectal but not testis temperature, thus demonstrating the ability of the conscious mouse to regulate the temperature of its testis.
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    Masthead (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980) 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250010401
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    Studies of exposure of rabbits to electromagnetic pulsed fields (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 345-352 
    Details
    ISSN: 0197-8462
    Keywords: electromagnetic pulsed (EMP) fields ; pentobarbital-induced sleeping time ; serum chemistry ; serum triglycerides ; creatine phosphokinase (CPK) ; Dutch rabbits ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Dutch rabbits were acutely exposed to electromagnetic pulsed (EMP) fields (pulse duration 0.4μs, field strengths of 1-2 kV/cm and pulse repetition rates in the range of 10 to 38 Hz) for periods of up to two hours. The dependent variables investigated were pentobarbital-induced sleeping time and serum chemistry (including serum triglycerides, creatine phosphokinase (CPK) isoenzymes, and sodium and potassium). Core temperature measured immediately pre-exposure and postexposure revealed no exposure-related alterations. Over the range of field strengths and pulse durations investigated no consistent, statistically significant alterations were found in the end-points investigated.
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    Plane-wave spectrum approach for the calculation of electromagnetic absorption under near-field exposure conditions (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 363-377 
    Details
    ISSN: 0197-8462
    Keywords: near field ; plane-wave exposure ; electromagnetic energy absorption ; homogeneous tissue slab ; fast Fourier transform ; 2450 MHz ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The exposure of humans to electromagnetic near fields has not been sufficiently emphasized by researchers. We have used the plane-wave-spectrum approach to evaluate the electromagnetic field and determine the energy deposited in a lossy, homogeneous, semi-infinite slab placed in the near field of a source leaking radiation. Values of the fields and absorbed energy in the target are obtained by vector summation of the contributions of all the plane waves into which the prescribed field is decomposed. Use of a fast Fourier transform algorithm contributes to the high efficiency of the computations. The numerical results show that, for field distributions that are nearly constant over a physical extent of at least a free-space wavelength, the energy coupled into the target is approximately equal to that resulting from plane-wave exposure.
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    Microwave radiation (2450 MHz) alters the endotoxin-induced hypothermic response of rats (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 353-361 
    Details
    ISSN: 0197-8462
    Keywords: endotoxin ; hypothermia ; rats ; 2450 MHz ; microwaves ; heating ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The parenteral administration of bacterial endotoxin to rats causes a hypothermia that is maximal after approximately 90 minutes. When endotoxin-injected rats were held in a controlled environment at 22°C and 50% relative humidity and exposed for 90 minutes to microwaves (2450 MHz, CW) at 1 mW/cm2, significant increases were observed in body temperature compared with endotoxintreated, sham-irradiated rats. The magnitude of the response was related to power density (10 mW/cm2 〉 5 mW/cm2 〉 1 mW/cm2). Saline-injected rats exposed for 90 minutes at 5 mW/cm2 (specific absorption rate approximately 1.0 mW/g) showed no significant increase in body temperature compared with saline-injected, sham-irradiated rats. The hypothermia induced by endotoxin in rats was also found to be affected by ambient temperature alone. Increases in ambient temperature above 22°C in the absence of microwaves caused a concomitant increase in body temperature. This study reveals that subtle microwave heating is detectable in endotoxin-treated rats that have an impaired thermoregulatory capability. These results indicate that the interpretation of microwave-induced biological effects observed in animals at comparable rates and levels of energy absorption should include a consideration of the thermogenic potential of microwaves.
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    Electromagnetic absorption in a multilayered slab model of tissue under near-field exposure conditions (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 379-388 
    Details
    ISSN: 0197-8462
    Keywords: near fields ; plane-wave spectrum (PWS) ; energy deposition ; layering resonance ; multilayered slab model ; plane wave ; fast Fourier transform ; 2,450 MHz ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The electromagnetic energy deposited in a semi-infinite slab model consisting of skin, fat, and muscle layers is calculated for both plane-wave and near-field exposures. The plane-wave spectrum (PWS) approach is used to calculate the energy deposited in the model by fields present due to leakage from equipment using electromagnetic energy. This analysis applies to near-field exposures where coupling of the target to the leakage source can be neglected. Calculations were made for 2,450 MHz, at which frequency the layered slab adequately models flat regions of the human body. Resonant absorption due to layering is examined as a function of the skin and fat thicknesses for plane-wave exposure and as a function of the physical extent of the near-field distribution. Calculations show that for fields that are nearly constant over at least a free-space wavelength, the energy deposition (for the skin, fat, and muscle combination that gives resonant absorption) is equal to or less than that resulting from plane-wave exposure, but is appreciably greater than that obtained for a homogeneous muscle slab model.
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    Effects of 2.45-GHz microwave radiation on embryonic quail hearts (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 389-396 
    Details
    ISSN: 0197-8462
    Keywords: microwaves ; nonionizing radiation ; embryogenesis ; enzymes ; cardiac muscle ; 2.45 GHz ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Although exposure to nonionizing electromagnetic radiation has been reported to cause a variety of systemic alterations during embryonic development, there are few reports of the induction of specific physiologic or morphologic changes in the myocardium. This study was designed to examine the effects of microwave radiation on cardiogenesis in Japanese quail embryos exposed during the first eight days of development to 2.45-GHz continuous-wave microwaves at power densities of 5 or 20 mW/cm2. The specific absorption rates were 4.0 and 16.2 mW/g, respectively. The ambient temperature for each exposure was set to maintain the embryonated eggs at 37.5 °C. This did not preclude thermal gradients in the irradiated embryos since microwaves may not be uniformly absorbed. The test exposure levels did not induce changes in either the morphology of the embryonic heart or the ultrastructure of the myocardial cells. Analysis of the enzymatic activities of lactate dehydrogenase, glutamic oxaloacetic transaminase, and creatine phosphokinase failed to reveal any statistically significant differences between the nonexposed controls and those groups exposed to either 5 or 20 mW/cm2. The data indicate that 2.45-GHz microwave radiation at 5 or 20 mW/cm2 has no effect on the measured variables of the Japanese quail myocardium exposed during the first eight days of development.
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    Referees for bioelectromagnetics, 1980 (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 429-431 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250010409
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    Alteration of circulating antibody response of mice exposed to 9-GHz pulsed microwaves (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 397-404 
    Details
    ISSN: 0197-8462
    Keywords: antibody response ; microwaves ; immunology ; 9-GHz pulsed radiation ; infectivity ; mouse ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A significant increase was observed in the circulating antibody titers of mice exposed to 9-GHz pulsed microwaves at an average power density of 10 mW/ cm2, two hours per day for five days compared with sham-irradiated animals. The mice were previously immunized with type III pneumococcal polysaccharide. Following irradiation, a portion of the immunized animals were challenged with virulent Streptococcus pneumoniae, type III. Ten days after challenge, mortality was essentially the same in the two groups, but during the ten day period, there was a noticeable increase in the survival time of the irradiated animals compared with the sham-irradiated animals, suggesting that the increased circulating antibody response afforded some degree of temporary protection to the animals.
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    Biologic effects of microwave exposure. II. Studies on the mechanisms controlling susceptibility to microwave-induced increases in complement receptor-positive spleen cells (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 405-414 
    Details
    ISSN: 0197-8462
    Keywords: 2450-MHz radiation ; complement receptors ; endotoxin ; T cells ; genetic control ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In attempting to evaluate the mechanisms responsible for susceptibility to the inductive increase in splenic complement receptor-positive (CR+) cells following exposure to 2450-MHz microwaves, it was found that sensitivity to microwave-induced CR+cell increases was under genetic control. In particular, evidence was accumulated suggesting that regulation was under the control of a gene or genes closely associated with but outside of the mouse major histocompatibility complex (H-2). All responsive strains of mice tested were of the H-2k haplotype, while mice of the H-2a, H-2b, H-2d and H-2i5 haplotypes were refractory to the microwave-induced increases in CR+ cells. By utilizing certain H-2k strains of mice that were genetically unable to respond to endotoxin, we were able to show that these strains of mice responded to microwaves, but not to endotoxin, by increasing CR+ cells. Microwave-induced increases in CR+cells were not mimicked by the intraperitoneal injection of hydrocortisone. Athymic mice responded to microwave exposure, indicating that this event was not regulated by the T-cell population. Mice less than eight weeks old were found not to be susceptible to exposure to 2450-MHz microwaves. These studies indicate that microwaves do induce changes in the population of cells with specific cell-surface receptors, that susceptibility to these changes is under genetic control, and that it is unlikely that endotoxin, corticosteroids, or regulatory T cells play a significant role in the mechanisms regulating these increases.
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    Masthead (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981) 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250020101
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    Microwave irradiation and instrumental behavior in rats: Unitized irradiation and behavioral evaluation facility (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 415-428 
    Details
    ISSN: 0197-8462
    Keywords: microwave radiation ; pulse-modulated ; operant behavior ; chronic irradiation ; waveguide exposure ; performance ; microwave dosimetry ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A facility for the exposure of small animals to pulse-modulated microwave radiation (PM MWR) concurrent with their performance of operant behavioral tasks is described. The computer-managed facility comprises an array of 32 individual waveguide exposure cells, each enclosing instrumental conditioning apparatus within a plastic subhousing. The distribution of the microwave electric field intensity within the waveguide was measured by a nonperturbing probe and the modifications induced by the behavioral apparatus and animal within the waveguide determined. Input and interior voltage standing wave ratios are presented to characterize the design of the chambers and to demonstrate the suitability of the chambers for whole-body irradiation of rat. The specific absorption rate (SAR) is presented utilizing data derived from incremental thermometric examination of saline loads and of selected sites in rat carcasses. This is compared with the whole-body SAR derived from the input/ output energy balance equation for the waveguide. The results of continuous monitoring of the SAR by the latter method, while unrestrained rats were engaged in operant and exploratory behavior within the waveguide, are utilized to derive a relationship between chamber input power and the dose rate for adult rats behaviorally active within the waveguide. From these data, we conclude that the experimental array provides a practical method for exposing a large number of animals to PM MWR for long periods of time and coincident with the establishment and/or performance of complex operant behavior.
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    Possible effects of 1011 Hz radiation on the oxygen affinity of hemoglobin (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 349-361 
    Details
    ISSN: 0197-8462
    Keywords: hemoglobin ; influence of radiation on oxygen affinity ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: When oxygen binds to one of the subunits of hemoglobin, the oxygen affinity of the other subunits is enhanced. This cooperative interaction of the subunits is initiated by the movement of the heme plane toward the proximal side when oxygen binds to the heme. This motion is transmitted to the surface of the globin through a “reaction channel” consisting of a group of atoms whose motion is well correlated. Considering the detailed geometry and X-ray diffraction data of the mean square displacement of the atoms surrounding the heme, a simple model for the heme plane oscillations is developed. Using this model, the natural frequency of oscillations is shown to be ≈5 × 1011 Hz. This result, along with the recent experimental data on the kinetics of the conformational changes of the heme, points to the possibility of radiation influencing the oxygen affinity of hemoglobin. If such an effect exists, it is likely that the oxygen affinity will be enhanced by the radiation.
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    Erratum (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 391-391 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/bem.2250030311
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    Assessment of the immune responsiveness of mice irradiated with continuous wave or pulse-modulated 425-MHz radio frequency radiation (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 467-470 
    Details
    ISSN: 0197-8462
    Keywords: 425 MHz ; mice ; continuous wave ; pulse modulated ; immune response ; lymphocytes ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Groups of female BALB/C mice were irradiated with 425-MHz radio frequency (RF) radiation either continuous wave (CW) or pulse modulated (PM, 1-ms pulse width, 250 pulses/s). Mice were irradiated in a rectangular strip-transmission line at average forward powers of 78, 17.7, or 5 W for CW and 17.7, 5, or 1.25 W for PM. The mean specific absorption rate, as measured using twin-well calorimetry was 7.7 W/kg for a forward power of 70 W. No differences in the mitogen-stimulated response of lymphocytes or in the primary antibody response to sheep erythrocytes or polyvinylpyrrolidone were observed between irradiated and sham-irradiated mice, nor between mice exposed to either CW or PM 425-MHz RF radiation.
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    URL: http://dx.doi.org/10.1002/bem.2250030409
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    Attempts to alter 45Ca2+ binding to brain tissue with pulse-modulated microwave energy (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 3 (1982), S. 475-478 
    Details
    ISSN: 0197-8462
    Keywords: brain calcium ; microwave effects on brain calcium ; microwave CNS effects ; pulse-modulated microwaves ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Rat brain tissue, loaded with 45Ca2+ by intraventricular injection was exposed in vitro to pulsemodulated 1-GHz (SAR of 0.29 or 2.9 W/kg) or 2.45-GHz radiation (SAR = 0.3 W/kg), and in vivo to 2.06-GHz radiation (SAR of 0.12 to 2.4 W/kg). There were no significant differences in efflux of 45Ca2+ between the microwave- and sham-irradiated groups.
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    Influence of in vitro microwave radiation on the fertilizing capacity of turkey sperm (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 4 (1983), S. 43-54 
    Details
    ISSN: 0197-8462
    Keywords: microwave radiation ; fertilizing capacity ; turkey sperm ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Turkey sperm were exposed to 2.45-GHz microwave radiation in a temperature-controlled waveguide apparatus. Temperature was maintained at either 25 or 40.5 °C. The sperm were exposed for 30 min at a specific absorption rate (SAR) of 10 or 50 mW/g. Following irradiation, the sperm were used to inseminate virgin turkey hens artificially. During the 9 weeks following the single insemination, the following were assessed: mean number of eggs, percentage of fertile eggs, rate of decrease in egg fertility, percentage of hatched eggs, and percentage of early and late deaths. These data demonstrate that, for the conditions used in these experiments, microwave radiation has no effect on the fertilizing capacity of turkey sperm.
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    Electromagnetic heating of tissue-equivalent phantoms with thin, insulating partitions (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 5 (1984), S. 221-232 
    Details
    ISSN: 0197-8462
    Keywords: electromagnetic power absorption ; tissue-equivalent phantoms ; heating distributions ; specific absorption rate (SAR) ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The electromagnetic power absorption in tissue-equivalent phantoms that are used for evaluation of diathermy and hyperthermia applicators is analyzed for the purpose of determining the effect of an insulating partition that is frequently used to facilitate separation of the phantom for thermographic analysis of heating distributions. An analysis that is based on the plane wave spectrum decomposition of the electromagnetic field is applied to a simplified model of the medium. The simplified model is valid whenever the insulating partition does not significantly alter the fields in the medium. The curves that are presented indicate that thin partitions do not significantly alter the power absorption for most situations of therapeutic interest. Data on the effects of partition thickness and electrical parameters are presented for microwave and radiofrequencies of interest for diathermy and hyperthermia.
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    URL: http://dx.doi.org/10.1002/bem.2250050210
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    Thermal action of 2.45 GHz microwaves on the cytoplasm of Chinese hamster cells (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 5 (1984), S. 247-261 
    Details
    ISSN: 0197-8462
    Keywords: 2.45-GHz radiation ; fluorescence polarization ; cytoplasm ; Chinese hamster cells ; microwaves ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In order to demonstrate possible specific effects of microwaves at the cellular level V-79 Chinese hamster cells were exposed to 2.45-GHz radiation at power levels of 20-200 mW/cm2 and at specific absorption rates of 10-100 mW/g. Intracellular cytoplasmic changes were observed by fluorescence polarization using a method based on the intracellular enzymatic hydrolysis of nonfluorescent fluorescein diacetate (FDA). At levels of absorbed energy below 90 J/g, modifications of microviscosity and mitochondrial state were absent, but a slight stimulation of enzymatic hydrolysis of FDA was observed which may be explained by microwave-induced alterations of cellular membranes possibly due to differences in heating pattern of microwaves compared to water-bath heating. At levels of absorbed energy above 90 J/g, the decrease of enzymatic hydrolysis of FDA, increase in degree of polarization, and increase of permeation of the fluorescent marker correlated well with the decrease in cell viability as measured by the exclusion of trypan blue. At equal absorbed energy, microwaves were found to exert effects comparable to classical heating except that permeation was slightly more affected by microwave than by classical heating. This suggests that membrane alteration produced by microwaves might differ from those induced by classical heating or that microwaves may have heated the membrane to higher temperatures than did classical heating.
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    Microwave bioeffects in the erythrocyte are temperature and pO2 dependent: Cation permeability and protein shedding occur at the membrane phase transition (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 5 (1984), S. 283-291 
    Details
    ISSN: 0197-8462
    Keywords: microwaves ; Na transport ; oxygen tension ; erythrocytes ; membrane phase transitions ; protein shedding ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Microwave exposure (2450 MHz, 60 mW/g, CW) of rabbit erythrocytes increases Na passive transport only at membrane phase transition temperatures (Tc) of 17-19°C. This permeability effect is enhanced for relative hypoxia which is characteristic of intracellular oxygen tension (pO2 ≤ 5 mm Hg). Neither the permeability nor the pO2 effects are observed in temperature-matched (± 0.05°C), sham-exposed controls. In addition, at Tc, microwave exposure is observed to induce the shedding or release of two erythrocyte proteins not seen in sham-exposed controls. Moreover, the enhanced shedding of at least seven other proteins all of molecular weight ≤ 28,000 D was detected in the microwave-treated samples. Using sensitive silver staining we estimate that approximately 450 fg of protein were shed per erythrocyte. These results demonstrate that temperature and pO2 are important influences on both functional and structural responses of cell membranes to microwave radiation.
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    Real-time measurement of RFR energy distribution in the macaca mulatta head (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 21-34 
    Details
    ISSN: 0197-8462
    Keywords: radiofrequency radiation ; microwaves ; rhesus monkey dosimetry ; microwave dosimetry ; cranial structures ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Temperature increases due to absorption of 1.2 GHz, CW, 70 mW/cm2, radio frequency (RF) energy, were measured in 3.3-cm-radius homogeneous muscle-equivalent spheres, M. mulatta cadaver heads (both detached from and attached to the body) and living, anesthetized M. mulatta heads. Temperatures were measured with a Vitek, Model 101 Electrothermia Monitor and temperature distributions were compared to theoretical predictions from a thermal-response model of a simulated cranial structure. The results show that the thermal response model accurately predicts the temperature distribution in muscle-equivalent spheres, the distribution of temperature in detached M. mulatta heads when exposed from the back of the head, and the distribution of temperature in attached M. mulatta cadaver heads for animals oriented with body parallel to the H-field. The temperature distribution in the detached M. mulatta heads varies markedly with exposure orientation, ie, facing forward, backward, or to the side. The orientation of the M. mulatta cadaver body significantly affects the temperature distribution in the head - with H-field orientation showing high, nonuniform values, and E-field orientation showing low, uniform values. In live animals blood flow produces a significant short-term effect on the temperature distribution in the midbrain, but not the cortex. Midbrain temperatures are both significantly higher and lower than the comparable cadaver measurements, depending on location.
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    Induction of calcium-ion efflux from brain tissue by radiofrequency radiation: Effect of sample number and modulation frequency on the power-density window (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 35-43 
    Details
    ISSN: 0197-8462
    Keywords: calcium ions ; brain tissue ; radiofrequency radiation ; amplitude modulation ; power-density window ; nonionizing radiation ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Changes have been found in calcium-ion binding to brain tissue exposed in vitro to a specific power density (0.83 mW/cm2) of 147-MHz radiation, amplitude modulated by a 16-Hz sine wave. This report replicates and extends this previous work. To define more precisely the range of effective power densities, two different numbers of samples were treated in a Crawford cell. In one series, four brain tissues were exposed at a time; in the other series, four brain tissues plus six dummy loads were exposed together. While the four-sample configuration produced a narrow power-density window, the ten pseudosample configuration resulted in a broader power-density window. The reason for the sample-number dependence is unresolved, but may be due to interactions between samples and field distortions caused by the close spacing. The ten pseudosample configuration was used to test for the presence and range of a power-density window at a sinusoidal modulation frequency of 9 Hz. The response curve at 9 Hz was essentially identical to the results for 16-Hz sinewave modulation.
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    Cardiovascular response of rats exposed to 60-Hz electric fields (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 55-64 
    Details
    ISSN: 0197-8462
    Keywords: 60-Hz electric fields ; electrocardiogram (ECG) ; heart rate ; blood pressure ; vascular reactivity ; cold stress ; rats ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Recently, it has been reported that exposure to high-strength electric fields can influence electrocardiogram (ECG) patterns, heart rates, and blood pressures in various species of animals. Our studies were designed to evaluate these reported effects and to help clarify some of the disagreement present in the literature. Various cardiovascular variables were measured in Sprague-Dawley rats exposed or sham-exposed to 60-Hz electric fields at 80 or 100 kV/m for periods up to four months. No significant differences in heart rates, ECG patterns, blood pressures, or vascular reactivity were observed between exposed and sham-exposed rats after 8 hours, 40 hours, 1 month, or 4 months of exposure. Blood pressure and heart rate measurements, made during exposure to a 100-kV/m electric field for one hour, revealed no significant differences between exposed and sham-exposed groups. In addition, physiologic reserve capacity, measured in rats subjected to low temperature after exposure to 100 kV/m for one month, showed that electric-field exposure had no significant effect on physiological response to cold stress. Our studies cannot be directly compared to the work of other investigators because of differences in animal species and electric-field characteristics. However, our failure to detect any cardiovascular changes may have been the result of 1) eliminating secondary field effects such as shocks, audible noise, corona, and ozone; 2) minimizing steady-state microcurrents between the mouth of the animal and watering devices; and 3) minimizing electric-field-induced vibration of the electrodes and animal cages.
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    Tests of mutagenesis and reproduction in male rats exposed to 2,450-MHz (CW) microwaves (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 65-76 
    Details
    ISSN: 0197-8462
    Keywords: mutagenesis ; reproduction ; rats ; 2,450 MHz ; microwaves ; continuous wave (CW) ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Tests of mutagenesis and reproduction were conducted in male rats which were irradiated by 2,450-MHz, continuous-wave (CW) microwaves, 4 hr/day from day 6 of gestation to 90 days of age at 5 mW/cm2; or 5 hr/day for five days beginning on the 90th day of age at 10 mW/cm2; or 4 hr/day, 5 days/ wk for four weeks, beginning on the 90th day of age. During selected weekly periods after treatment, the rats were bred to pairs of untreated, normal female rats that were examined in late pregnancy by means of the dominant lethal assay. The reproductive efficiency of these males, as reflected in their breeding, was also examined for changes relating to their microwave experience. No significant evidence of germ-cell mutagenesis was detected when data of microwave-exposed males were compared with those of sham-exposed males, even though there were significant increases in rectal and intra-testicular temperatures at a power density of 28 mW/cm2. Temporary sterility, as indexed by fewer pregnancies, was seen at the highest power density.
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    Immunologic and hematopoietic alterations by 2,450-MHz electromagnetic radiation (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 77-87 
    Details
    ISSN: 0197-8462
    Keywords: mouse spleen lymphocytes ; marrows ; 2,450 MHz ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A biphasic modulation of responsiveness of spleen lymphocytes to mitogens was observed in mice exposed to 2,450-MHz radiation at power densities of 5-15 mW/cm2 over various periods ranging between one and 17 days. This modulated phenomenon may be explained on the basis of 1) suppression of lymphocyte response by microwave-activated macrophages which persists throughout the entire course of radiation, and 2) concurrent progressive direct stimulation of lymphocytes which culminates around day 9 of exposure. Tumor cytotoxicity of killer lymphocytes from mice exposed to five or nine days of radiation did not appear different from sham controls. The highly proliferative hematopoietic marrow cells were sensitive to microwave radiation. Nine days of exposure to radiation (15 mW/cm2) reduced the colonyforming units of myeloid and erythroid series by 50%. This observation may offer a new and more sensitive assay for studying biological effects of electromagnetic radiation.
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    Masthead (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980) 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250010201
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    Modification of the repeated acquisition of response sequences in rats by low-level microwave exposure (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 89-99 
    Details
    ISSN: 0197-8462
    Keywords: repeated acquisition ; microwave radiation ; lever press ; rats ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The acute effects of microwave exposure on a repeated acquisition baseline were investigated in three rats. Each session the animals acquired a different four-member response sequence. Each of the first three correct responses advanced the sequence to the next member, and the fourth correct response produced food reinforcement. Incorrect responses produced a three-second timeout. Baseline and control sessions were characterized by a decrease in errors within each session. The animals were acutely exposed to a 2.8 GHz pulsed-microwave field prior to test sessions, with average power densities ranging from 0.25 to 10 mW/cm2. In comparison to control sessions, 1/2 hour of exposure to microwave radiation at power densities of 5 and 10 mW/cm2 increased errors and altered the pattern of within-session acquisition. Exposure to the 10 mW/cm2 power density decreased the rate of sequence completion in all animals. The results of exposures at 0.25, 0.5, and 1 mW/cm2 power densities were generally within the control range. The results are interpreted as indicating a disruption in the discriminative stimulus control of the repeated acquisition behavior.
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    Failure of rats to escape from a potentially lethal microwave field (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 101-115 
    Details
    ISSN: 0197-8462
    Keywords: escape ; microwaves ; rats ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Ocularly pigmented rats, all mature females of the Long-Evans strain, were repeatedly presented an opportunity to escape from an intense 918-MHz field (whole-body dose rate = 60 mW/g) to a field of lower intensity (40, 30, 20, or 2 mW/g) by performing a simple locomotor response. Other rats could escape 800-μA faradic shock to the feet and tail by performing the same response in the same milieu, a multimode cavity. None of 20 irradiated rats learned to associate entry into a visually well-demarcated area of the cavity with immediate reduction of dose rate, in spite of field-induced elevations of body temperature to levels that exceeded 41°C and would have been lethal but for a limit on durations of irradiation. In contrast, all of ten rats motivated by faradic shock rapidly learned to escape. The failure of escape learning by irradiated animals probably arose from deficiencies of motivation and, especially, sensory feedback. Whole-body hyperthermia induced by a multipath field may lack the painful or directional sensory properties that optimally promote the motive to escape. Moreover, a decline of body temperature after an escape-response-contingent reduction of field strength will be relatively slow because of the large thermal time constants of mammalian tissues. Without timely sensory feedback, which is an essential element of negative reinforcement, stimulus-response associability would be imparied, which could retard or preclude learning of an escape response.
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    Studies on microwave and blood-brain barrier interaction (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 1 (1980), S. 313-323 
    Details
    ISSN: 0197-8462
    Keywords: microwave ; blood-brain barrier ; Evans blue ; sodium fluorescein ; brain and body temperatures ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: This investigation was aimed at correlating changes of blood-brain-barrier permeability with the quantity and distribution of absorbed microwave energy inside the brain of adult Wistar rats anesthetized by sodium pentobarbital. Through use of thermographic methods and a direct-contact applicator at the animal's head, the pattern of absorbed microwave energy was determined. Indwelling catheters were placed in the femoral vein and in the left external carotid artery. Evans blue and sodium fluorescein in isotonic saline were used as visual indicators of barrier permeation. Exposure to pulsed 2,450-MHz radiation for 20 min at average power densities of 0.5, 1, 5, 20, 145 or 1,000 mW/cm2, which resulted in average specific absorption rates (SARs) of 0.04, 0.08, 0.4, 1.6, 11.5 or 80.0 mW/g in the brain, did not produce staining, except in the pineal body, the pituitary gland, and the choroid plexus  -  regions that normally are highly permeable. Except for these regions, staining was also absent in the brains of sham-exposed animals. The rectal temperature, as monitored by a copper-constantan thermocouple, showed a maximum increase of less than 0.75°C from a mean pre-exposure temperature of 36.6°C. The highest brain temperature recorded in a similar group of animals using a thickfilm carbon thermistor was less than 41.0°C.
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    URL: http://dx.doi.org/10.1002/bem.2250010307
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    General properties of the interaction between animals and ELF electric fields (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 1-11 
    Details
    ISSN: 0197-8462
    Keywords: electric fields ; 60-Hz ; biological effects ; dosimetry ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: An analysis is given of the interaction between extremely low-frequency (ELF) electric fields and animals of arbitrary body shape. This analysis is based on three approximations which are valid in the ELF range: In living tissues, capacitive (displacement) currents are negligible compared to conduction currents; effects resulting from the finite velocity of propagation of electromagnetic fields are negligible; skin effect in living tissues is negligible. Major conclusions of the analysis are: (a) The electric field outside the body, the induced charge on the surface of the body, and the total current crossing any section through the body (eg, through the neck or limbs) are completely determined by the characteristics of the applied ELF electric field, the shape of the body, its location relative to ground and other conductors, and any conduction currents from the body to ground or other conductors. (b) All of the quantities in (a) can be measured using conducting animal models. (c) The magnitudes of the electric field outside the body and the induced charge density on the surface of the body are independent of frequency, in the ELF range, when the body is either insulated from or shorted to ground (and any other conductors in the system). (d) The only quantities affected by the electrical properties of the tissues comprising the body are the current density and electric field inside the body. (e) The electric field outside and inside a body will be unchanged by a scaled change in its size.
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    URL: http://dx.doi.org/10.1002/bem.2250020102
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    Biological effects of oscillating electric fields: Role of voltage-sensitive ion channels (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 23-32 
    Details
    ISSN: 0197-8462
    Keywords: biological effects of oscillating electric fields ; excitable cells ; voltage-sensitive ion channels ; gating charges ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: An alternating component of potential across the membrane of an excitable cell may change the membrane conductance by interacting with the voltagesensing charged groups of the protein macromolecules that form voltage-sensitive ion channels. Because the probability that a voltage sensor is in a given state is a highly nonlinear function of the applied electric field, the average occupancy of a particular state will change in an oscillating electric field of sufficient magnitude. This “rectification” at the level of the voltage sensors could result in conformational changes (gating) that would modify channel conductance. A simplified two-state model is examined where the relaxation time of the voltage sensor is assumed to be considerably faster than the fastest changes of ionic conductance. Significant changes in the occupancy of voltage sensor states in response to an applied oscillating electric field are predicted by the model.
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    URL: http://dx.doi.org/10.1002/bem.2250020104
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    Fluorescence depolarization studies of red cell membrane fluidity. The effect of exposure to 1.0-GHz microwave radiation (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 13-22 
    Details
    ISSN: 0197-8462
    Keywords: Crawford cell ; diphenylhexatriene ; fluorescence depolarization ; microviscosity ; 1-GHz microwave radiation ; red cell membranes ; temperature dependence ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The internal viscosity of human red blood cell membranes was investigated during exposure to continuous wave 1.0-GHz microwave radiation using fluorescence measurements of a lipid seeking molecular probe, diphenylhexatriene. Samples were exposed in a Crawford cell arranged so that fluorescence was measured during microwave exposure; specific absorption rates calculated from electrical measurements were approximately 0.6, 2 and 15 W/kg. Measurements were obtained at selected temperatures between 15 °C and 40 °C and as a function of the duration of exposure at 23 °C. Arrhenius-type plots of the temperature profile data were linear and showed no difference between exposed and control samples. The exposure duration data also showed no difference between exposed and control samples except for a small effect of elevated temperature at the highest exposure. The activation energy for motion of the fluorescent probe in its environment within the membrane lipid was not affected by the application of the microwave energy and no evidence for a lipid phase transition was found. These results indicate that the increased cation efflux from red cells, observed by others at certain transition temperatures during microwave exposure, was more likely to have been caused by alteration of the membrane bound protein than by changes in the lipid constituents of the red cell membrane.
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    Interactive effects in 60-HZ electric-field exposure systems (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 33-50 
    Details
    ISSN: 0197-8462
    Keywords: electric fields ; 60-Hz ; dosimetry ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The level of exposure of laboratory animals to 60-Hz electric fields is commonly specified in terms of the unperturbed field strength present before the introduction of experimental subjects and their cages. In the research reported in this paper, rats were housed in two parallel rows of 12.4 cm × 25.1 cm × 10.2 cm high plastic cages resting on the lower electrode of a parallel-plate exposure system, and the actual perturbed electric fields experienced by an experimental animal were investigated. The most important results are: 1) Reducing the spacing between the exposure electrodes from 8.7 to 1.7 times the height of a singly exposed rat model, while maintaining a constant unperturbed field strength, resulted in a 15% increase in the electric field at the highest point on the surface of the body and a 10% increase in the short-circuit current of the model. 2) For multiple animal exposures, increases of 10% in both the field at the highest point of the body and the short-circuit current were observed when the electrode spacing was reduced from 8.7 to 2.6 times the height of a rat. 3) Plastic cages caused 1 - 6% reductions in the electric field at the surface of the body, except very near the cage walls, where enhancements of more than 20% were observed. 4) When 16 rats were simultaneously exposed, the short-circuit current, Is, of an individual subject of weight W (in g), that was surrounded on all sides by other rats of weight W, was reduced from the short-circuit current, Iu, measured with the same subject individually exposed as follows: during a 12 h light (sleeping) cycle, Is/Iu = 1.00 - 0.0173W1/2; during a 12 h night (awake) cycle, Is/Iu = 1.00 - 0.0136W1/2.
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    URL: http://dx.doi.org/10.1002/bem.2250020105
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    Radiofrequency electromagnetic fields in swedish radio stations and tall FM/TV towers (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 61-69 
    Details
    ISSN: 0197-8462
    Keywords: FM radio broadcast ; VHF-TV broadcast ; UHF-TV broadcast ; electromagnetic field strengths ; personnel hazard ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Radiofrequency electric and magnetic fields have been measured around 11 large broadcast stations and tall FM/TV towers in Sweden. The results show that operating personnel may be exposed to fields exceeding by several times the present standard for occupational exposure to RF radiation. Maintenance personnel are especially vulnerable to exposure when climbing energized towers. The present study indicates that the transmitters should be switched off during the performance of certain tasks on the tower. Safe passage of maintenance personnel near energized antennas of certain types is impossible without a drastic reduction of the transmitted power. In the stations, the RF leakage radiation was generally low, but when work was done with the cabinet doors of the transmitter open, high field strengths were found in the vicinity of the transmitter even though it was switched off. It was found that the transmitter acted as a tuned receiver of energy from the other transmitters.
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    Flight, orientation, and homing abilities of honeybees following exposure to 2.45-GHz CW microwaves (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 71-75 
    Details
    ISSN: 0197-8462
    Keywords: honeybees ; invertebrates ; behavior ; solar power satellite ; 2.45-GHz microwaves ; continuous wave ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Foraging-experienced honeybees retained normal flight, orientation, and memory functions after 30 minutes' exposure to 2.45-GHz CW microwaves at power densities from 3 to 50 mW/cm2. These experiments were conducted at power densities approximating and exceeding those that would be present above receiving antennas of the proposed solar power satellite (SPS) energy transmission system and for a duration exceeding that which honeybees living outside a rectenna might be expected to spend within the rectenna on individual foraging trips. There was no evidence that airborne invertebrates would be significantly affected during transient passage through microwaves associated with SPS ground-based microwave receiving stations.
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    Microwave-induced increase of water and conductivity in submaxillary salivary gland of rats (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 51-60 
    Details
    ISSN: 0197-8462
    Keywords: 2880-MHz microwaves ; rats ; submaxillary salivary gland ; thermoregulation ; tissue conductivity ; tissue hydration ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Hypersalivation is an important mechanism for heat dissipation by animals without sweat glands. The water content and conductivity (at 20 kHz) in sub-maxillary salivary gland (SSG) and in other tissues were investigated in adult male rats exposed to microwaves (2880 MHz, 1.5 μs pulses at 1000 Hz) or to conventional heat at 40 °C. Eighty rats in one series were exposed, one at a time, for 30 min to microwaves producing a specific absorption rate (SAR) of 4.2,6.3,6.8,8.4,10.8 or 12.6 W/kg. Fifty rats were sham-exposed under similar environmental conditions. In the second series, ten rats were sham-exposed, 33 rats were exposed, one at time, for 15, 30 or 60 min to microwaves at a SAR of 9.5 W/kg, and 32 rats were exposed for similar periods to conventional heat at 40 °C. In rats of the first series colonic temperatures were elevated significantly at a SAR of 4.2 W/kg, while SSG water content and conductivity increased significantly at SAR values of 6.3 W/kg and higher. In the second series of experiments increases in colonic temperature and SSG water content were greater after 15 and 30 min of microwave exposure than after exposure to heat. Also, SSG conductivity was significantly depressed by heat and significantly increased by microwaves after exposure for 15 or 30 min. The results support the hypothesis that water content and conductivity of SSG of rats can be used as a sensitive specific test of a microwave induced thermal response.
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    Microwave treatment of intracerebral l1210 leukemia: Schedule-dependent partial reversal of the effects of methotrexate (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 77-80 
    Details
    ISSN: 0197-8462
    Keywords: microwaves ; L1210 leukemia ; central nervous system ; methotrexate ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: One-GHz microwave (MW) irradiation at a power density of 5 mW/cm2 was combined with methotrexate (MTX) in an attempt to treat more effectively central nervous system (CNS) L1210 leukemia in DBA/2J mice. When mice with CNS leukemia were treated with the combination of MW and MTX, there was no improvement in survival compared with a group of animals treated with MTX alone; however, the group that received MTX before the MW exposure had a significantly reduced survival time compared with the group treated with MTX alone or with the group to which MTX was administered after MW.
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    Masthead (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981) 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250020201
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    Possible humoral mechanism of 2450-MHz microwave-induced increase in complement receptor positive cells (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 81-84 
    Details
    ISSN: 0197-8462
    Keywords: 2450-MHz microwaves ; complement receptors ; diffusion chambers ; soluble factors ; immune system ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: These studies indicate that the increase in the frequency of complement receptor-positive (CR+) spleen cells observed 6 days after a 30-min exposure to 2450-MHz microwaves is not the result of microwave-induced alterations of lymphocyte recirculation patterns, but is mediated by a soluble, humoral factor produced by cells within the spleen.
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    Dielectric properties of animal tissues in vivo at frequencies 10 MHz - 1 GHz (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 93-103 
    Details
    ISSN: 0197-8462
    Keywords: permittivity ; in vivo ; radio frequencies ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: An open-ended coaxial line sensor in conjunction with an automatic network analyzer was used to measure in vivo the permittivity of several feline tissues (skeletal and smooth muscle, liver, kidney, spleen, and brain - gray and white matter) at frequencies between 10 MHz and 1 GHz. The estimated uncertainties of measurement were between 1.5% and 5%. The data are in general agreement with previously obtained data in vitro and in vivo. Significant differences in the properties of different types of the same tissue (eg, skeletal and smooth muscle) were observed. Many tissues were found to be non-homogeneous in its permittivity.
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    Endocrinological effects of strong 60-Hz electric fields on rats (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 105-121 
    Details
    ISSN: 0197-8462
    Keywords: 60-Hz electric fields ; ELF radiation ; rat endocrinology ; organ weights ; hormones ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Adult male rats were exposed or sham-exposed to 60-Hz electric fields without spark discharges, ozone, or significant levels of other secondary variables. No effects were observed on body weights or plasma hormone levels after 30 days of exposure at an effective field strength of 68 kV/m. After 120 days of exposure (effective field strength = 64 kV/m), effects were inconsistent, with significant reductions in body weight and plasma levels of follicle-stimulating hormone and corticosterone occurring in one replicate experiment but not in the other. Plasma testosterone levels were significantly reduced after 120 days of exposure in one experiment, with a similar but not statistically significant reduction in a replicate experiment. Weanling rats, exposed or sham-exposed in electric fields with an effective field strength of 80 kV/m from 20 to 56 days of age, exhibited identical or closely similar growth trends in body and organ weights. Hormone levels in exposed and sham-exposed groups were also similar. However, there was an apparent phase shift between the two groups in the cyclic variations of concentrations of hormones at different stages of development, particularly with respect to follicle-stimulating hormone and corticosterone. We concluded that 60-Hz electric fields may bring about subtle changes in the endocrine system of rats, and that these changes may be related to alterations in episodic rhythms.
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    Effect of homogeneous magnetic fields on responses to toxic stimulation in spirostomum ambiguum (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 187-198 
    Details
    ISSN: 0197-8462
    Keywords: ciliated protozoan ; DC magnetic field ; calcium-mediated contraction ; motility ; 2,2′-dipyridyldisulfide ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Effects of DC magnetic fields, up to 125,000 G, on the survival of the ciliated protozoan Spirostomum ambiguum exposed to the toxic substance 2,2′-dipyridyldisulfide are reported. The magnetic field diminishes the ability of the organism to survive the drug, and lengthens the extension phase of the contraction cycle. It is proposed that the magnetic field alters the regulation of intracellular (C2+) transients.
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    Masthead (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981) 
    Details
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250020301
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    Development of xenopus laevis embryos in a static magnetic field (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 199-201 
    Details
    ISSN: 0197-8462
    Keywords: static magnetic fields ; development ; frog embryos ; low temperature ; cell membranes ; lipid phase transition ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Xenopus laevis embryos were exposed to a DC magnetic field (2.5 kG) for periods up to 1 week. The previously reported stabilization of cell membranes by stationary magnetic fields could not be demonstrated.
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    Prolonged microwave irradiation of rats: Effects on concurrent operant behavior (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 169-185 
    Details
    ISSN: 0197-8462
    Keywords: microwave radiation ; pulse modulation ; waveguide exposure ; chronic exposure ; operant behavior ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Two measures of performance were used to study the effects of pulse-modulated microwave radiation (PM MWR) on schedule-controlled operant behavior of rats: 1) cued (SD), fixed-ratio (FR) bar pressing for food reinforcement; and 2) noncued (Sd) bar pressing in the absence of food reinforcement. The animals were irradiated and the behavioral data were obtained concurrently, during daily three-hour sessions, five days per week for six to nine weeks. Each experiment began with a two to three-week baseline interval of sham irradiation; a two to three-week interval of sham irradiation followed the irradiation phase. The irradiated animals were exposed to 1.3-Ghz PM MWR (pulse width of 1 microsecond at 600 pulses per second) at whole-body, average specific absorbed-dose rates of from 1.5-6.7 mW/g. Control and irradiated animals were tested in identical, cylindrical waveguide exposure/behavioral assemblies; different groups of irradiated and sham-irradiated animals were used for each dose rate. At 1.5 mW/g, the levels of SD operant responding by control and irradiated animals were comparable, and showed similar progressive diminutions over the course of each daily session. Sd operant responding was more variable, but again comparable, with both groups showing similar, progressive declines in rate of responding during each session. At 3.6 mW/g, no specific effects on SD operant response rates were observed. However, there was an initial and transient increase in the rate of extinction of Sd responding. At 6.7 mW/g, SD response rates were slightly reduced, whereas there was a major reduction in noncued (Sd) operant responding followed by a sharp rebound during the first post-MWR week. This marked reduction in Sd operant responding at MWR onset was in contrast to the relative stability and persistence of FR responding for food reinforcement.
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    Transbilayer movement of 24Na in sonicated phosphatidylcholine vesicles exposed to frequency-modulated microwave radiation (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 217-225 
    Details
    ISSN: 0197-8462
    Keywords: lipid vesicles ; Na+ leakage ; 2450 MHz ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Sonicated egg phosphatidylcholine vesicles loaded with 24Na+ were exposed at 20mW to a frequency-modulated (3 Hz) microwave field in the range of 2350 to 2550 MHz, or at 80 mW to a 2450-MHz CW (continuous wave) field, in a waveguide. The vesicle suspension absorbed microwaves at about 1 mW/ml and 25 mW/ml (CW experiment). The average temperature change of the irradiated suspension was 〈 0.1 °C from ambient. Leakage of 24Na+ from the vesicles for up to 19 hours was measured. No difference was noted in the movement of 24Na+ from the vesicles in the irradiated and control dispersions.
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    In vitro microwave effects on human neutrophil precursor cells (CFU-C) (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 203-215 
    Details
    ISSN: 0197-8462
    Keywords: 2,450-MHz microwaves ; neutrophil ; colony-forming cells ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Human marrow cells were irradiated with 2450-MHz CW microwaves in a fluid-filled waveguide irradiation system. Cell exposure was conducted by placing a marrow cell suspension in 20-μl glass microcapillary tubes that were positioned in the exposure chamber, and irradiated at power densities from 31 to 1,000 mW/cm2 (with corresponding specific absorption rates of 62 to 2,000 mW/g) for 15 minutes. The temperature of the sample was maintained at a fixed point. Sham-irradiated (SI) and microwave-irradiated (MWI) cells were cultured in a methylcellulose culture system for neutrophil colony proliferation. There was no reduction in neutrophil colony number on days 6-7 or 12-14 in cells exposed at 31 or 62 mW/cm2, but as the power density was increased to 1,000 mW/cm2, there was a reduction in colony number of MWI cells compared with SI cells. The microwave interaction with the human neutrophil colony-forming cells was apparently not related to temperature rise, or to the state of cell cycle, and was irreversible.
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    Chronic exposure to a 60-Hz electric field: Effects on neuromuscular function in the rat (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 227-239 
    Details
    ISSN: 0197-8462
    Keywords: 60-Hz electric fields ; rats ; soleus muscle ; plantaris muscle ; neuromuscular function ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Neuromuscular function in adult male rats was studied following 30 days of exposure to a 60-Hz electric field at 100 kV/m (unperturbed field strength). Isometric force transducers were attached to the tendons of the plantaris (predominantly fast twitch), and soleus (predominantly slow twitch) muscles in the urethan-anesthetized rat. Square-wave stimuli were delivered to the distal stump of the transected sciatic nerve. Several measurements were used to characterize neuromuscular function, including twitch characteristics, chronaxie, tetanic and posttetanic potentiation, and fatigue and recovery. The results from three independent series of experiments are reported. Only recovery from fatigue in slow-twitch muscles was consistently and significantly affected (enhanced) by electrifield exposure. This effect does not appear to be mediated by field-induced changes in either neuromuscular transmission, or in the contractile mechanism itself. It is suggested that the effect may be mediated secondary to an effect on mechanisms regulating muscle blood flow or metabolism.
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    Effect of nonionizing radiation on the purkinje cells of the uvula in squirrel monkey cerebellum (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 241-246 
    Details
    ISSN: 0197-8462
    Keywords: monkey cerebellum ; Purkinje cells ; nonionizing radiation ; microwaves ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Pregnant squirrel monkeys were exposed to 2450-MHz (CW) microwaves at an equivalent power density of 10 mW/cm2 (SAR 3.4 mW/g) for three hours daily in a cavity-cage module. The exposure began when pregnancy was determined by a hormonal method, and continued through the offspring's first 9.5 months. After irradiation, the brains of the offspring were fixed with formaldehyde, and the inferior vermis of each cerebella was removed and processed for histologic observations. Purkinje cell density in the uvula was determined in sagittal serial sections. There was no significant difference between control and experimental animals in the number of Purkinje cells per mm of Purkinje cell line (linear density), as well as in the density of Purkinje cells in the Purkinje cell layer.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250020305
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  • 199
    Electronic Resource
    Electronic Resource
    The mechanisms of athermal microwave biological effects (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 259-267 
    Details
    ISSN: 0197-8462
    Keywords: Biological effects ; microwave ; athermal ; exposure ; non-ionizing radiation ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A brief survey of current ideas about the physical mechanisms of low-level millimeter/microwave-biological effects is given, and the experimental evidence supporting these ideas is reviewed. The conjectural models do not yet represent a complete physical theory, but they do counter the idea that low-level effects are physically impossible and suggest experiments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250020307
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  • 200
    Electronic Resource
    Electronic Resource
    Effect of nonionizing radiation on the purkinje cells of the rat cerebellum (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 247-257 
    Details
    ISSN: 0197-8462
    Keywords: nervous system ; rat cerebellum ; nonionizing radiation ; Purkinje cells ; microwaves ; radiofrequency ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In one experiment, Sprague Dawley rats (16-21 days of gestation) and their offspring were exposed to 100-MHz (CW) electromagnetic radiation at 46 mW/cm2 (SAR 2.77 mW/g) for 4 h/day for 97 days. In another experiment, the pregnant rats were irradiated daily from 17 to 21 days of gestation with 2450-MHz (CW) microwaves at 10 mW/cm2 (SAR 2 mW/g) for 21 h/day. In a third experiment, 6-day-old rat pups were irradiated 7 h/day for five days with 2450-MHz radiation at 10 mW/cm2. Equal numbers of animals were sham irradiated in each group. Quantitative studies of Purkinje cells showed a significant and irreversible decrease in rats irradiated during fetal or fetal and early postnatal life. In animals exposed postnatally, and euthanized immediately after irradiation, significant decrease in the relative number of Purkinje cells was apparent. However, restoration apparently occurred after forty days of recovery.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250020306
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