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  • 1
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    Entomologia experimentalis et applicata 85 (1997), S. 231-236 
    ISSN: 1570-7458
    Keywords: Glossina fuscipes fuscipes ; vegetation ; biconical trap ; temperature ; light ; relative humidity ; monitor lizard
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Glossina fuscipes fuscipes Newstead was sampled in isolated thickets and forest patches near Lake Victoria, Kenya using unbaited biconical traps, between March 1992 and June 1993. Traps set at 1 m from the forest edge caught 3.3 times as many males and 5 times as many females as those set inside or 10 m away. The corresponding figures at 1 m from the edge of thicket were about 1.43 and 1.64 times, respectively. Hourly catches of males and females were positively correlated with temperature, light intensity and host (monitor lizard) prevalence, and negatively correlated with relative humidity. Light intensity and temperature were the most important variables affecting the catches of each sex. The results are discussed in relation to control and monitoring of G. f. fuscipes using traps.
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  • 2
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    Entomologia experimentalis et applicata 83 (1997), S. 171-180 
    ISSN: 1570-7458
    Keywords: within-plant heterogeneity ; tent caterpillars ; temperature ; circular statistics ; Lepidoptera ; Yponomeutidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The caterpillars of Yponomeuta mahalebella Latr. (Lepidoptera: Yponomeutidae) are monophagous on Prunus mahaleb (Rosaceae) leaves. Adult females deposit eggs in batches, which determines larval gregarious behaviour. Coupled with gregarious behaviour, caterpillars spin silk tents within they will feed until pupation. Distribution of tents in the field, their effect in microenvironmental larval growth conditions and the consequences for adult body mass and survival of larvae were studied. There was a significant trend for tents to be placed with a southward orientation. Within-plant tent orientation was related to the sun-shade pattern experienced, which was the main effect determining thermal differences between the inside and the outside of the tent. These differences appeared to affect larval growth and survival, with higher survival of larvae and heavier adults emerging from tents oriented to the south-east and east, respectively.
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  • 3
    ISSN: 1572-8099
    Keywords: point of fire origin ; fire ; temperature ; plaster ; concrete ; thermal ; damage ; biotite ; magnetization ; susceptibility ; N-rays ; P-rays ; X-rays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying
    Notes: Abstract Magnetic susceptibility (MS) measurements were taken to determine fire exposure patterns on noncombustible structural members and compartment boundaries. These measurements led on-site fire investigators to the cause of combustion and the point of fire origin. Thermal damage to walls and to ceilings was estimated quantitatively by measuring MS and two-valent Fe ion concentration in building materials such as concrete and plaster. It was shown that these effects are preconditioned by thermochemical transformations in the mineral biotite, which is present as a component of common building materials. For the first time, the effect of a substantial and irreversible increase in biotite magnetization exposed to powerful flows of N- and P-rays was found. It is hoped that the presence of this “magnetic trace” of radiation in building materials will allow investigators to use the magnetometric method at nuclear power plant catastrophes such as that in Chernobyl. It was also shown that the influence of N- and P-rays on biotite's magnetic properties greatly differs from the thermal influence on biotite.
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  • 4
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    Entomologia experimentalis et applicata 82 (1997), S. 159-166 
    ISSN: 1570-7458
    Keywords: Diaeretiella rapae ; reproduction ; life-tables ; temperature ; Diuraphis noxia ; biological control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diaeretiella rapae (M'Intosh), a polyphagous and cosmopolitan parasite of aphids, was imported from China to the USA for biological control of Diuraphis noxia (Mordwilko), a pest of small grains. We studied several aspects of its biology on D. noxia hosts in the laboratory at 3 constant temperatures, 10.0, 21.1, and 26.7 °C. Females lived significantly longer than males at all 3 temperatures, and longevity was greatest at 10 °C, followed by 21.1 and 26.7 °C. Fecundity (= number of mummies) did not differ significantly among the 3 temperatures studied. The ovarian egg-load was 129.1 ± 9.3, and was significantly affected by the size of adult females. A pre-oviposition period ± 0.26 days) was detected at 10 °C, but not at the other temperatures. Pre-imaginal survivorship was similar among temperatures, while R o and T c decreased with temperature, and r m increased with temperature. The offspring sex ratio (proportion females) was lowest at 26.7 °C, and similar between 10.0 and 21.1 °C. In addition, the offspring sex ratio significantly declined with the age of the female parent. The suitability of D. rapae for colonization against D. noxia in North America is discussed in relation to its responses to temperature and the climate of its home range.
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  • 5
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    Entomologia experimentalis et applicata 82 (1997), S. 319-333 
    ISSN: 1570-7458
    Keywords: chlorogenic acid ; light availability ; Lycopersicon esculentum ; Manduca sexta ; protein ; rutin ; temperature ; tomatine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined how light availability influenced the defensive chemistry of tomato (Lycopersicon esculentum: Solanaceae). Tomato plants were grown either in full sunlight or under shade cloth rated at 73%. Leaves from plants grown in full sunlight were tougher, had higher concentrations of allelochemicals (chlorogenic acid, rutin and tomatine), and had less protein than leaves from plants grown in shade. We determined how these differences in host plant quality due to light availability affected the behavior and growth of a Solanaceae specialist, Manduca sexta. Both in the greenhouse and in the field, caterpillars on shade-grown plants grew heavier in a shorter amount of time than those on plants that had previously been grown in full sunlight. In contrast, the effects of previous light availability to plants on caterpillar behavior appeared to be minor. To further investigate how light availability to plants influenced herbivore growth, we examined the effects of leaf-powder diets made from tomato leaves of different ages (new, intermediate, or mature) grown in full sunlight or shade on caterpillar performance. Caterpillars fed diets made from plants grown in shade consumed less but grew faster than larvae fed diets made from tomato plants grown in full sunlight. Caterpillars fed diets made from new leaves grew larger in less time than caterpillars fed diets made from intermediate aged leaves. Caterpillars did not survive on the mature leaf powder diets. There were plant-light treatment by larval thermal regime interactions. For example, at 26:15 °C , plant-light treatment had no effect on stadium duration, but at 21:10 °C, stadium duration was prolonged with the full sunlight-new leaf diet compared with the shaded-new leaf diet. In a second diet experiment, we examined the interactive effects of protein and some tomato allelochemicals (rutin, chlorogenic acid and tomatine) on the performance of caterpillars. There were food quality by thermal regime interactions. For instance, at 26:15 °C , neither protein nor allelochemical concentration influenced stadium duration, whereas at 21:10 °C, stadium duration was prolonged with the low protein-high allelochemical diet, which simulated full sunlight leaves. In sum, light availability to plants affected defensive chemistry and protein concentration. The difference in food quality was great enough to influence the growth of a specialist insect herbivore, but the effects were temperature-dependent.
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  • 6
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    Entomologia experimentalis et applicata 84 (1997), S. 255-265 
    ISSN: 1570-7458
    Keywords: Delia radicum ; postdiapause development ; temperature ; Diptera ; Anthomyiidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Canadian populations of D. radicum differ in their response to temperature during postdiapause development. Populations that are primarily of the early-emerging type ( $$\left( { \leqslant 256DD_{04} } \right)$$ ) (St-Jean, Quebec; London, Ontario) have high values for the parameters describing this response: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaamOuamaaBa% aaleaacaWGTbaabeaakiabg2da9aaa!38F2!$$R_m = $$ 12.7–13.3; $$T_m = $$ 28.0–31.8 °C ; Tσ = 10.3–14.2 ( $$R_m $$ , the maximum developmental rate at the temperature, $$T_m $$ [ °C ] where the developmental rate is highest, and Tσ , the parameter which gives the shape of the truncated normal curve fitted to the data), a low degree-day requirement for emergence (160–232 $$DD_{04} $$ ), and may lack a developmental delay at temperatures above ca. 21 °C . Populations of the late-emerging type (Kildare, Prince Edward Island) have low parameter values ( $$ R_m = 2.5$$ , $$T_m = 19.3$$ °C ; Tσ = 6.4), high degree-day requirements (530 $$DD_{04} $$ ), and a developmental delay at high temperatures. The parameters for the early-emergers in the population from Winnipeg, Manitoba (74% early) were intermediate ( $$R_m = 9.1$$ , $$T_m = 27.1$$ °C , Tσ = 10.7, $$ DD_{04} = 246 $$ ), but resembled the early rather than the late type. This population varied from 31 to 90% early type over a 10-year period and the rate of postdiapause development at 20 °C was directly related to the percentage early. In the year with the most rapid development (90% early), development was significantly slower than in the populations from other locations with predominantly early populations, and the year with the slowest development (31% early) showed significantly faster development than that from Kildare, Prince Edward Island (100% late). Therefore the parameters for early and late types of development will not be accurate for use in mixed populations, and the parameters in mixed populations will change among years. Populations of D. radicum in North America and Europe (67 locations by years) varied from 0–100% early. At Winnipeg, the percentage early was directly related to the annual temperature accumulation ( $$DD_{05} $$ ) during the growing season. The calculation of developmental parameters for the early-emergers of mixed populations provides a more accurate basis for estimating the times of first emergence and the first peak of emergence than parameters based on the whole population. Since postdiapause developmental rates vary both among and annually within locations, developmental models should be designed to include such variations.
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  • 7
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    Journal of applied electrochemistry 27 (1997), S. 1328-1332 
    ISSN: 1572-8838
    Keywords: metal hydride ; capacity ; temperature ; performance ; enthalpy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract The effect of temperature on the performance of a LaNi4.76Sn0.24 metal hydride electrode was investigated in the temperature range of 0 to 50°C. The electrode showed a maximum discharge capacity at 25°C. The total resistance increases with a decrease of temperature from 50°C to 0°C. The apparent activation enthalpies at different states of charge were determined by evaluating the polarization resistance at different temperatures. It was found that the apparent activation enthalpy is an indicator of the relative reaction rate of the charge-transfer reaction and hydrogen absorption.
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  • 8
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    Positivity 1 (1997), S. 319-330 
    ISSN: 1572-9281
    Keywords: delay equations ; stability ; positive solutions ; spectral growth condition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We prove stability for a semilinear delay equation, whose nonlinearity is majorized by a linear positive operator. The key ingredients are a spectral condition, positivity of solutions to the linear problem, and lattice properties of the Banach space.
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  • 9
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    Set-valued analysis 5 (1997), S. 73-88 
    ISSN: 1572-932X
    Keywords: differential inclusion ; invariance ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract The properties of invariance, stability, asymptotic stability and attainability of a given compact set $$K \subset \mathbb{R}^n $$ with respect to a differential inclusion, have weak and strong versions: the weak version requires existence of a trajectory with the corresponding property, while the strong one requires this property for all trajectories. The following statement is proven in the paper (under slight restrictions) for each of the above-mentioned properties: if K has the weak property with respect to $$\dot x \in F(x) $$ , then there is a (regulation) mapping G such that G(x) ⊂ F(x) ∀ x and G has the strong property with respect to $${\dot x}$$ ε G(x). In addition, certain regularity of the set of solutions of the last inclusion is claimed.
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    Set-valued analysis 5 (1997), S. 365-375 
    ISSN: 1572-932X
    Keywords: set-valued mappings ; vector optimization ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We establish optimization results for set-valued mappings, with the image space given by a topological vector space partially ordered by a cone. Moreover, we obtain stability results relative to parametrized optimization problems. We use a weak semicontinuity concept related to the order structure of the image space and show how compactness assumptions used in previous papers can be lightened.
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  • 11
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    Queueing systems 27 (1997), S. 205-226 
    ISSN: 1572-9443
    Keywords: multiclass queueing networks ; ergodicity ; stability ; performance analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract We develop the use of piecewise linear test functions for the analysis of stability of multiclass queueing networks and their associated fluid limit models. It is found that if an associated LP admits a positive solution, then a Lyapunov function exists. This implies that the fluid limit model is stable and hence that the network model is positive Harris recurrent with a finite polynomial moment. Also, it is found that if a particular LP admits a solution, then the network model is transient.
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  • 12
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    Journal of dynamics and differential equations 9 (1997), S. 463-505 
    ISSN: 1572-9222
    Keywords: Difference equations ; random perturbation ; averaging ; diffusion approximation ; randomly perturbed iterations ; stability ; 3SR60 ; 60H15 ; 60J99
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Let (X, ℬ) and (Y,C) be two measurable spaces withX being a linear space. A system is determined by two functionsf(X): X→ X andϕ:X×Y→X, a (small) positive parameterε and a homogeneous Markov chain {y n } in (Y,C) which describes random perturbations. States of the system, say {x n ɛ ∈X, n=0, 1,⋯}, are determined by the iteration relations:x n+1 ɛ =f(x n ɛ )+ɛϕ(x n ɛ ,Yn+1) forn≥0, wherex 0 ɛ =x 0 is given. Here we study the asymptotic behavior of the solutionx n ɛ asε → 0 andn → ∞ under various assumptions on the data. General results are applied to some problems in epidemics, genetics and demographics.
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    Set-valued analysis 5 (1997), S. 377-390 
    ISSN: 1572-932X
    Keywords: differential inclusions ; stability ; boundedness of solutions ; Lyapunov functions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract For Lipschitzian differential inclusions, we prove that the existence of suitable Lyapunov functions is necessary for uniform stability and uniform boundedness of solutions.
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  • 14
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    Queueing systems 26 (1997), S. 343-363 
    ISSN: 1572-9443
    Keywords: retrial queues ; stability ; ergodicity ; renovation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract We consider the following Type of problems. Calls arrive at a queue of capacity K (which is called the primary queue), and attempt to get served by a single server. If upon arrival, the queue is full and the server is busy, the new arriving call moves into an infinite capacity orbit, from which it makes new attempts to reach the primary queue, until it finds it non-full (or it finds the server idle). If the queue is not full upon arrival, then the call (customer) waits in line, and will be served according to the FIFO order. If λ is the arrival rate (average number per time unit) of calls and μ is one over the expected service time in the facility, it is well known that μ 〉 λ is not always sufficient for stability. The aim of this paper is to provide general conditions under which it is a sufficient condition. In particular, (i) we derive conditions for Harris ergodicity and obtain bounds for the rate of convergence to the steady state and large deviations results, in the case that the inter-arrival times, retrial times and service times are independent i.i.d. sequences and the retrial times are exponentially distributed; (ii) we establish conditions for strong coupling convergence to a stationary regime when either service times are general stationary ergodic (no independence assumption), and inter-arrival and retrial times are i.i.d. exponentially distributed; or when inter-arrival times are general stationary ergodic, and service and retrial times are i.i.d. exponentially distributed; (iii) we obtain conditions for the existence of uniform exponential bounds of the queue length process under some rather broad conditions on the retrial process. We finally present conditions for boundedness in distribution for the case of nonpatient (or non persistent) customers.
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    Annals of global analysis and geometry 15 (1997), S. 277-297 
    ISSN: 1572-9060
    Keywords: mean curvature ; $$r$$ -mean curvature ; sphere ; stability ; stable
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We deal with compact hypersurfaces immersed in space forms with constant $$r$$ -mean curvature. They are critical points for a variational problem. We show they are stable if and only if they are geodesic spheres, generalizing results on constant curvature hypersurfaces.
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  • 16
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    Acta applicandae mathematicae 49 (1997), S. 35-54 
    ISSN: 1572-9036
    Keywords: dynamical systems ; stability ; pseudo orbit tracing property ; nonstandard analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract It is known that it is not possible to introduce C0 -structural stability for whole systems in topological dynamics. Using the methods of Nonstandard Analysis, we suggest four different purely topological stability concepts for dynamical systems on compact subsets of Rn. Classically these amount to considering the space of all systems on a given subset of Rn as the fundamental entity when deforming a continuous system (instead of the space of all continuous systems as is normally done in topological dynamics). For two of the introduced stability concepts, we will show that all minimal flows are stable in this sense. Besides this, we will show that one of our stability concepts is related to what is called the pseudo orbit tracing property in a recently published book by Aoki and Hiraide and compare some of our results to the theory of dynamical systems as presented there.
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    Celestial mechanics and dynamical astronomy 67 (1997), S. 181-204 
    ISSN: 1572-9478
    Keywords: Hamiltonian systems ; symplectic mappings ; normal forms ; resonances ; stability ; three degrees of freedom
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We analyze four-dimensional symplectic mappings in the neighbourhood of an elliptic fixed point whose eigenvalues are close to satisfy a third-order resonance. Using the perturbative tools of resonant normal forms, the geometry of the orbits and the existence of elliptic or hyperbolic one-dimensional tori (fixed lines) is worked out. This allows one to give an analytical estimate of the stability domain when the resonance is unstable. A comparison with numerical results for the four-dimensional Hénon mapping is given.
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    Celestial mechanics and dynamical astronomy 69 (1997), S. 271-281 
    ISSN: 1572-9478
    Keywords: restricted three-body problem ; libration points ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The existence and stability of triangular libration points in the relativistic restricted three-body problem has been studied. It is found that L4,5 are unstable in the whole range 0 ≤ µ ≤ 1/2 in contrast to the classical restricted three-body problem where they are stable for 0 〈 µ 〈 µ0, where µ is the mass parameter and µ0 = 0.03852....
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    Celestial mechanics and dynamical astronomy 69 (1997), S. 317-330 
    ISSN: 1572-9478
    Keywords: artificial satellite ; Nekhoroshev's theory ; normal form ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We investigate the significance of long time stabilty predictions in the light of Nekhoroshev's theory by studying the orbits of artificial satellites. As a simplified model problem we consider the so-called J2problem for an earth's satellite, neglecting luni-solar perturbations and nonconservative effects. We consider a wide range of orbits, excluding those which are too close to the critical inclination. Most of the orbits turn out to be stable for times larger than the estimated age of the solar system, thus proving that, as far as dissipation can be neglected, stability in Nekhoroshev's sense may be effective for physically realistic systems.
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    Archive of applied mechanics 67 (1997), S. 447-456 
    ISSN: 1432-0681
    Keywords: Key words rotation ; stability ; energy criterion ; variational analysis ; functional analysis ; eigenvalue problem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary Stability of a heavy rotating rod with a variable cross section is studied by energy method. Bifurcation points for the system of equilibrium equations are analyzed. It is shown that for the case when the rotation speed exceeds the critical one, the trivial solution ceases to be the minimizer of the potential energy, so that rod loses stability, according to the energy criteria. Also, a new estimate of the maximal rod deflection in the post-critical state is obtained.
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    Biology and fertility of soils 25 (1997), S. 163-168 
    ISSN: 1432-0789
    Keywords: Key words Arbuscular mycorrhiza ; Ectomycorrhiza ; Benomyl ; Colonization ; External hyphae ; Field study ; Glomales ; Zea mays ; Pisolithus tinctorius
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Arbuscular mycorrhizal (AM) grasses compete for nutrients with ectomycorrhizal (EM) pine in the southeastern United States. Our objective was to determine if benomyl could be used to selectively inhibit the function of AM and thereby reduce grass competition in the field. The effects of Benlate (active ingredient: benomyl) in the greenhouse and field were evaluated. No effect was observed on pine inoculated with Pisolithus tinctorius in the greenhouse. Colonized root length of benomyl-treated Zea mays L. plants inoculated with Glomus sp. in the greenhouse remained static over time and the response was not dose dependent at concentrations of 0, 20, 60 and 150kg benomyl ha–1 equivalent. In contrast, colonization of nontreated plants increased over time. In the field, a minimal reduction of grass colonization was observed following four applications of benomyl ranging from 5 to 20kgha–1. We conclude that benomyl can successfully inhibit development of AM fungi under controlled conditions in the greenhouse with no inhibitory effects on the EM fungus P. tinctorius; however, in the field several factors may interfere with the effect of benomyl on AM fungi. These factors include: (a) the presence of ground cover which obstructs penetration of the fungicide to the soil, (b) timing of application in relation to mycorrhizal development, and (c) the application method of benomyl, a soil drench being preferable to a foliar spray.
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    Biology and fertility of soils 25 (1997), S. 142-146 
    ISSN: 1432-0789
    Keywords: Key words Denitrification ; Tillage systems ; Maize ; Zea mays ; Hot spots ; Denitrifying populations ; N2O emission
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Denitrification losses show an irregular pattern through the year, often being caused by climatic conditions and management practices. The objectives of the present work were to quantify denitrification losses and to determine the influence of tillage system on the factors that control denitrification in fertilized soils. The modal profile of the soil was an Vertic Argiudoll, clay loam texture, located in Buenos Aires province, Argentina. The treatments were: (a) fertilized, (b) incorporated fertilization and (c) without fertilization for both no tillage and conventional tillage systems. Chambers were placed in the field to measure denitrification. In this clayish soil the estimated mean values of accumulated denitrification during the crop cycle (90 days) were 0.190kgNha–1 for conventional tillage and 0.350kgNha–1 for no tillage. In treatments with no tillage, losses by denitrification were approximately twice those of conventional tillage. These differences were also evidenced by the number of microorganisms, which were significantly higher (P〈〉;5%) for no tillage on all dates, except for at flowering. The increase at flowering coincided with the period of highest rainfall and consequently the highest water contents in the soil. The highest denitrification losses, except for sowing, were measured when soil moisture content was more than 30% (v/v). Denitrification increased in conjunction with an increase in the availability of carbon that is consumed by the heterotrophic microorganisms (including the denitrifiers).
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  • 23
    ISSN: 1432-0789
    Keywords: Key wordsAlternaria alternata ; Arbuscular mycorrhizas ; Fusarium equiseti ; Glomus mosseae ; Lactuca sativa ; Lettuce ; Maize ; Saprophytic fungi ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of inoculation with the saprophytic fungi Alternaria alternata or Fusarium equiseti on maize (Zea mays) and lettuce (Lactuca sativa) with or without arbuscular mycorrhizal (AM) colonization by Glomus mosseae was studied in a greenhouse trial. Plant dry weights of non-AM-inoculated maize and lettuce were unaffected by the presence of A. alternata and F. equiseti. In contrast, A. alternata and F. equiseti decreased plant dry weights and mycorrhization when inoculated to the rhizosphere before G. mosseae. The saprophytic fungi inoculated 2 weeks after G. mosseae did not affect the percentage of root length colonized by the AM endophyte, but did affect its metabolic activity assessed as succinate dehydrogenase activity. Although F. equiseti inoculated at the same time as G. mosseae did not affect mycorrhization of maize roots, its effect on AM colonization of lettuce roots was similar to that with A. alternata. In the rhizosphere of both plants, the population of saprophytic fungi decreased significantly, but was not affected by the presence of G. mosseae. Our results suggest that there may have been a direct effect of the saprophytic fungi on the mycorrhizal fungi in the extramatrical phase of the latter, and when the AM fungus was established in the root the AM fungus was less affected by the saprophytic fungi.
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  • 24
    ISSN: 1432-0789
    Keywords: Key words Corn stubble decomposition ; N-fertilization ; Carbon light fraction ; Humic acids ; Microbial activity ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The variation in stubble decomposition due to fertilizer incorporation was determined in a typical Argiudoll of the Argentinian rolling pampas. The experiment was conducted for 15 years, which included a no till system under maize (Zea mays L.) and a soybean rotation (Glycine max) with 0 and 45kg N ha–1 nitrogen fertilization treatments, called NFS and FS, respectively. A higher proportion of residues with a high N content was found in the FS plots. The different substrate quality proved to be the regulating factor for mineralization. This activity was indicated by the increase in soil microbial activity and soil carbon light fraction in FS compared to NFS. This carbon light fraction mineralizes rapidly but does not contribute to the most stable components, which are related to synthesis and polymerization of humic acids. No significant differences in humic acid content were found.
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  • 25
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    Biology and fertility of soils 24 (1997), S. 266-273 
    ISSN: 1432-0789
    Keywords: Key words Green manure ; Lime ; K deficiency ; Oxisol ; Sweet corn ; Zea mays ; Mn toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Effects of local green manure (GM) and lime on soil productivity in a low-input agricultural system were evaluated by growing three successive crops of sweet corn (Zea mays) on an acid Oxisol (Typic acrorthox, Togitogiga series) in Western Samoa. The soil was amended with coral lime at 0, 5, and 10Mgha–1 and with cowpea GM at 0, 7.5, and 15Mgha–1. Commercial NPK fertilizers at 50kgha–1 each of N, P, and K were included for comparison. The amendments were applied only once prior to planting of the first crop. Response parameters measured included nutrient composition of leaves at tasseling and grain yield of each crop, and selected soil chemical properties at each planting. Yields of the first crop were nearly tripled with GM additions and doubled with lime additions. Such yield increases were caused mainly by better K nutrition and to a lesser extent by enhanced P nutrition. Yields of subsequent crops were much lower than those of the first, and the declines were much steeper for the GM treatments than for the lime treatments. Thus, the enhancement effect on K nutrition did not last beyond one crop. Poor growth of the second and third crops was caused by K deficiency; probably coupled with Mn toxicity. Significant yield reductions were found when Mn-to-K ratios in leaves exceeded 0.010. As for effects on soil, soil pH was increased significantly by lime but only slightly by GM. Given the variable charge property of this Oxisol, each unit pH increase corresponds to a cation exchange capacity (CEC) increase of 5cmolckg–1. Having greater CEC, the amended soil retained K more effectively, thereby causing yield increases, especially of the first corn crop, which required at least 0.75cmolckg–1 of exchangeable soil K or 7% of CEC for adequate growth.
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  • 26
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    Archive of applied mechanics 67 (1997), S. 331-338 
    ISSN: 1432-0681
    Keywords: Key words frictional heating ; wear ; contact pressure ; temperature ; brake system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary A transient contact problem with frictional heating and wear for two nonuniform sliding half-spaces is considered. One of the two half-spaces is assumed to be slightly curved to give a Hertzian initial pressure distribution: the other is a rigid nonconductor. Under the assumption that the contact pressure distribution could be described by Hertz formulas during all the process of interaction, the problem is formulated in terms of one integral equation of Volterra type with unknown radius of contact area. A numerical solution of this equation is obtained using a piecewise-constant presentation of an unknown function. The influence of operating parameters on the contact temperature and the radius of the contact area is studied.
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  • 27
    ISSN: 1573-0832
    Keywords: Anthracnose ; guava ; Colletotrichum glososporioides ; temperature ; free moisture ; inoculum density
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    Topics: Biology , Medicine
    Notes: Abstract The influence of environmental factors (temperature and humidity), inoculum density on infection by Colletotrichum glososporioides and development of anthracnose lesions were determined on uninjured, sand-injured and punctured fruits. The optical temperature for severe infection was 30 °C, whereas the disease incidence was less at 20 and 35 °C. Inoculated guavas that received 1–60 h of continuous free moisture developed lesions, but the disease was minimal (0–7%) after 1–6 h free moisture. Infection rates of uninjured, sand-injured and punctured fruits receiving 60 h of free moisture were 34, 70 and 100%, respectively. Disease incidence increased as inoculum density increased from 101 to 106 conidia/ml. In field conditions, the development of anthracnose lesions was greater on punctured guavas than on uninjured or sand-injured ones, in both rainy and winter seasons. In general, the number of lesions was highest in sand-injured fruits, followed by punctured and uninjured fruits. In rainy season the number of lesions on injured and uninjured fruits was greater than similarly treated guavas in winter.
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  • 28
    ISSN: 1573-0832
    Keywords: Antagonism ; biocontrol ; ginger ; postharvest rot ; Sclerotium rolfsii ; Trichoderma ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Sclerotium rolfsii is a destructive soil-borne and postharvest plant pathogen. Use of the antagonistic fungus Trichoderma sp. has been earlier reported by us to successfully control this pathogen under postharvest conditions. In the present paper we report on the effects of temperature on the growth and biocontrol potential of Trichoderma sp. on S. rolfsii. Experimental results indicated that S. rolfsii and Trichoderma sp. have different temperature optima for growth: 30–35 °C for the pathogen and 25–30 °C for the antagonist. In dual culture, Trichoderma overgrew S. rolfsii at 25 °C and 30 °C, but at 35 °C and 37 °C, S. rolfsii overgrew the colony of Trichoderma. Trichoderma produced higher concentration of fungitoxic metabolites in broth culture at higher temperatures. In bioassays using ginger slices and whole rhizomes, it has been demonstrated that Trichoderma is not very effective in suppressing S. rolfsii at temperatures above 30 °C. In light of these results, possible mechanisms of biocontrol of S. rolfsii as a postharvest pathogen has been discussed. Storage temperature has been suggested as a critical factor in biocontrol of S. rolfsii.
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  • 29
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    Journal of statistical physics 88 (1997), S. 691-711 
    ISSN: 1572-9613
    Keywords: Quasicrystals ; nonperiodic tilings ; classical lattice-gas models ; ground states ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We give strong evidence that noncrystalline materials such as quasicrystals or incommensurate solids are not exceptions, but rather are generic in some regions of phase space. We show this by constructing classical lattice-gas models with translation-invariant finite-range interactions and with a unique quasiperiodic ground state which is stable against small perturbations of two-body potentials. More generally, we provide a criterion for stability of nonperiodic ground states.
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  • 30
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    Journal of intelligent and robotic systems 20 (1997), S. 131-155 
    ISSN: 1573-0409
    Keywords: robot adaptive control ; basis function-like networks ; stability ; discrete variable structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Stable neural network-based sampled-data indirect and direct adaptivecontrol approaches, which are the integration of a neural network (NN)approach and the adaptive implementation of the discrete variable structurecontrol, are developed in this paper for the trajectory tracking control ofa robot arm with unknown nonlinear dynamics. The robot arm is assumed tohave an upper and lower bound of its inertia matrix norm and its states areavailable for measurement. The discrete variable structure control servestwo purposes, i.e., one is to force the system states to be within the stateregion in which neural networks are used when the system goes out of neuralcontrol; and the other is to improve the tracking performance within the NNapproximation region. Main theory results for designing stable neuralnetwork-based sampled data indirect and direct adaptive controllers aregiven, and the extension of the proposed control approaches to the compositeadaptive control of a flexible-link robot is discussed. Finally, theeffectiveness of the proposed control approaches is illustrated throughsimulation studies.
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    Journal of intelligent and robotic systems 19 (1997), S. 411-436 
    ISSN: 1573-0409
    Keywords: assembly planning ; stability ; robot ; forward ; operations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The paper presents an approach to sequence planning consisting in determining assembly sequences defined in terms of mating and non-mating operations and based on a dynamic expansion of the assembly tree obtained using a knowledge base management system. The planner considers the case of a single-robot assembly workcell. The use of stability and the detailed definition of sequences also by means of several non-mating operations are shown to be powerful instruments in the control of the tree expansion. Forward assembly planning has been chosen, in order to minimize the number of stability checks. Backtracking is avoided by combining precedence relations and stability analysis. Hard and soft constrains are introduced to drive the tree expansion. Hard constraints are precedence relations and stability analysis. All operations are associated to costs, which are used as soft constraints. The operation based approach enables one to manage even non-mating operations and to easily overcome the linearity constraint. Costs enable the planner to manage the association among tools and components. The first section of the paper concerns Stability Analysis that is subdivided into Static and Dynamic Stability Analysis. The former is mainly involved in analyzing gravity effects; the latter is mainly involved in evaluate inertia effects due to manipulation. Stability Analysis is implemented in a simplified form. Fundamental assumptions are: no rotational equilibrium condition is considered; for each reaction force only direction and versus, but not magnitude, are considered; friction is neglected. The second section discusses the structure of the planner and its implementation. The planner is a rule based system. Forward chaining and hypothetical reasoning are the inference strategies used. The knowledge base and the data base of the system are presented and the advantages obtained using a rule based system are discussed. The third section shows two planning examples, showing the performance of the system in a simple case and in an industrial test case, the assembly of a microwave branching filter composed of 26 components.
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    Journal of thermal analysis and calorimetry 49 (1997), S. 857-862 
    ISSN: 1572-8943
    Keywords: analytical methods ; kinetics ; powder milk ; temperature
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    Topics: Chemistry and Pharmacology
    Notes: Abstract This work present comparative results on powder milk storage quality, obtained from analytical methods. Protein content was determined conventional (Kjeldahl) and colorimetric with biuret reagent at 540 nm and integral quality by thermogravimetric and biological methods. A method was developed for the protein separation of powder milk. Powder milk was submitted to degradation processes at 45, 60 and 80°C for 20 days. The results indicated that protein content values were inconsistent if determinations by Kjeldahl and colorimetric methods and biological tests were compared. There is evidence of thermal decomposition of powder milk as detected by biological and thermogravimetric methods.
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    Journal of thermal analysis and calorimetry 49 (1997), S. 1501-1507 
    ISSN: 1572-8943
    Keywords: calcium sulphide ; gypsum ; oxidation ; phosphogypsum ; reduction ; stability ; thermogravimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Using a heating rate of 2°C min−1, CaS reacts with oxygen in air from 700°C to form CaSO4, with a complete conversion at 1100°C. Synthesis of CaS from the reaction between CaSO4 containing compounds and carbon compounds in air would not be possible, as the carbon reacts from 600°C with oxygen in the air to give CO2. Heating stoichiometric amounts of carbon and pure CaSO4, synthetic gypsum or phosphogypsum in a nitrogen atmosphere, results in the formation of CaS from 850°C. Using a heating rate of 10°C min−1, the formation of CaS is completed at 1080°C. Addition of 5% Fe2O3 as a catalyst lowers the starting temperature of the reaction to 750°C. Activation energy values at different fraction reaction values (α) differ between 340 and 400 kJ mol−1. The relationship between the activation energy values and conversion (α) indicates that the reaction proceeds via multiple steps.
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    Journal of thermal analysis and calorimetry 50 (1997), S. 625-632 
    ISSN: 1572-8943
    Keywords: DSC ; NMR ; 8-quinolinol compounds ; TG-DTG ; stability ; thermal decomposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Solid M-Ox compounds, whereM represents Mg(II), Zn(II), Pb(II) and NbO(III), and Ox is 8-quinolinol, have been prepared. Thermogravimetry, derivative thermogravimetry (TG, DTG), differential scanning calorimetry (DSC), nuclear magnetic resonance (NMR) and infrared absorption spectra (IR) have been used to characterize and to study the thermal stability and thermal decomposition of these compounds.
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    Journal of thermal analysis and calorimetry 50 (1997), S. 807-814 
    ISSN: 1572-8943
    Keywords: DTA ; stability ; substituted InF3 glasses
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The thermal properties and devitrification behaviour of substituted InF3 glasses were studied by means of differential thermal analysis. A comparison of various simple quantitative methods to assess the level of stability of multicomponent fluoride glass systems was also made. Most of these methods are based on critical temperatures. In this paper, a new parameter,k d(T), is introduced to the stability criteria. The stabilities of several substituted InF3 glasses were evaluated experimentally and correlated with the activation energies of crystallization via this new kinetic criterion and compared with those evaluated by other criteria.
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    Journal of nondestructive evaluation 16 (1997), S. 91-100 
    ISSN: 1573-4862
    Keywords: LASER ultrasound ; anisotropic material ; group velocity ; stiffness coefficients ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Ultrasonic waves are generated through a composite material by means of a noncontact technique. It uses a Nd:Yag LASER for the generation and an interferometric probe for the detection of acoustic waveforms. From a suitable set of experimental data, an inversion scheme is used for the recovering of four stiffness coefficients. They characterize the elasticity in a principal plane of symmetry of the material which exhibits an orthorhombic symmetry. The measurements are performed at various temperatures, elevated by steps up to 300°C for two specimen. The sensitivity of the method appears convenient to measure the temperature induced stiffness changes. The anisotropic degradation of the material properties are then pointed out.
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  • 37
    ISSN: 1573-4919
    Keywords: heart mitochondria ; lability ; muscle mitochondria ; oxidative phosphorylation ; stability ; taurine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We modified the isolation procedure of muscle and heart mitochondria. In human muscle, this resulted in a 3.4 fold higher yield of better coupled mitochondria in half the isolation time. In a preparation from rat muscle we studied factors that affected the stability of oxidative phosphorylation (oxphos) and found that it decreased by shaking the preparation on a Vortex machine, by exposure to light and by an increase in storage temperature. The decay was found to be different for each substrate tested. The oxidation of ascorbate was most stable and less sensitive to the treatments. When mitochondria were stored in the dark and the cold, the decrease in oxidative phosphorylation followed first order kinetics. In individual preparations of muscle and heart mitochondria, protection of oxidative phosphorylation was found by adding candidate stabilizers, such as desferrioxamine, lazaroids, taurine, carnitine, phosphocreatine, N-acetylcysteine, Trolox-C and ruthenium red, implying a role for reactive oxygen species and calcium-ions in the in vitro damage at low temperature to oxidative phosphorylation. In heart mitochondria oxphos with pyruvate and palmitoylcarnitine was most labile followed by glutamate, succinate and ascorbate.We studied the effect of taurine, hypotaurine, carnitine, and desferrioxamine on the decay of oxphos with these substrates. 1 mM taurine (n = 6) caused a significant protection of oxphos with pyruvate, glutamate and palmitoylcarnitine, but not with the other substrates. 5 mM L-carnitine (n = 6), 1 mM hypotaurine (n = 3) and 0.1 mM desferrioxamine (n = 3) did not protect oxphos with any of the substrates at a significant level. These experiments were undertaken in the hope that the in vitro stabilizers can be used in future treatment of patients with defects in oxidative phosphorylation. (Mol Cell Biochem 174: 61–66, 1997)
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    Journal of sol gel science and technology 8 (1997), S. 523-527 
    ISSN: 1573-4846
    Keywords: UF membranes ; alumina ; zirconia coating ; stability
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    Topics: Chemistry and Pharmacology
    Notes: Abstract For improving chemical and thermal stability of γ -Al2O3 membranes boehmite (AlOOH) sol-particles are coated with Zr4+-species with two techniques. These techniques are heterogeneous precipitation (HP-method) and “surface-reaction-followed-by-polycondensation” (SRPC-method). A continuous coating layer is formed at relative low Zr4+-concentration, about one monolayer coverage of boehmite particles, and with the HP-method. For larger concentrations and for the SRPC-method small particles (order 1 nm) of Zr4+-species are formed on the surface of the boehmite particles. After drying and calcination up to 1000°C no continuous layer of a zirconia phase could be detected for all samples. However the thermal stability of the porous structure is improved. Phase transitions of alumina occur at temperatures of 1100°C and the porous structure of the membrane material is then destabilized.
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    Journal of sol gel science and technology 8 (1997), S. 523-527 
    ISSN: 1573-4846
    Keywords: UF membranes ; alumina ; zirconia coating ; stability
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    Topics: Chemistry and Pharmacology
    Notes: Abstract For improving chemical and thermal stability of γ-Al2O3 membranes boehmite (AlOOH) sol-particles are coated with Zr4+-species with two techniques. These techniques are heterogeneous precipitation (HP-method) and “surface-reaction-followed-by-polycondensation” (SRPC-method). A continuous coating layer is formed at relative low Zr4+-concentration, about one monolayer coverage of boehmite particles, and with the HP-method. For large concentrations and for the SRPC-method small particles (order 1 nm) of Zr4+-species are formed on the surface of the boehmite particles. After drying and calcination up to 1000°C no continuous layer of a zirconia phase could be detected for all samples. However the thermal stability of the porous structure is improved. Phase transitions of alumina occur at temperatures of 1100°C and the porous structure of the membrane material is then destabilized.
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  • 40
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    Plant molecular biology 34 (1997), S. 383-392 
    ISSN: 1573-5028
    Keywords: maize ; mitochondrion ; RNA b ; RNA plasmids ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cytoplasm maize mitochondria. A soluble complex of RNA b, responsible for synthesis of the more abundant (+) RNA b strand in mitochondrial lysates, was identified. The complex had a buoyant density of 1.49 g/cm3, indicating a substantial non-nucleic acids content. The sedimentation coefficient of the complex, however, was only slightly larger than that of deproteinized RNA b. Synthesis of RNA b as well as the larger RNA plasmid, RNA a, was resistant to heparin, suggesting that, for both RNAs, preformed complexes between an RNA template and an RNA-dependent RNA polymerase capable of elongating in vivo preinitiated RNA plasmid strands, were present in the lysate. Only a small fraction of RNA b molecules were bound in the complex; the bulk of RNA b sedimented at the same rate as the deproteinized RNA. Thus, after replication, maize mitochondrial plasmids are not associated with nucleoprotein capsids although their synthesis takes place through ribonucleoprotein replication complexes.
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  • 41
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    Theoretical and applied genetics 95 (1997), S. 525-531 
    ISSN: 1432-2242
    Keywords: Key words Cytoplasmic male sterility ; Mitochondria ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Cytoplasmic male sterility (CMS) is the maternally inherited inability to produce functional pollen. The Rf3 allele of the nuclear gene rf3 gametophytically restores male fertility to maize plants with the S-type of CMS. The rf3 locus is on the long arm of maize chromosome two (2L). Using 2L RFLPs and three-point mapping analysis we showed that the rf3 locus is located an estimated 4.3 cM distal to the whp locus and 6.4 cM proximal to the bnl17.14 locus. This information was used in combination with RFLPs on two additional maize chromosomes to show that Rf3/rf3 CMS-S plants may aberrantly transmit the nonrestoring allele, rf3, through the male gametophyte.
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  • 42
    ISSN: 1871-4528
    Keywords: skin resistance ; temperature ; Solanum tuberosum L.
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Chambers were designed to simulate environmental conditions present in commercial potato stores and were used to introduce condensation to tubers infected with silver scurf (Helminthosporium solani). The electrical resistance measured across the skin of tubers from the top of the chambers was consistently lower than that from the bottom of the chamber, and this was reflected in an increased number ofH. solani spores produced by the top tubers. An increase in the length of the condensation period resulted in the skin resistance remaining lower for a longer period and also resulted in higher number of spores. Tubers held at relatively high ambient temperatures (15 °C) required a shorter period of condensation to cause an increase in spore count. This investigation highlighted the need for close monitoring of store environments so that condensation events can be quickly identified and the tubers dried.
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  • 43
    ISSN: 1871-4528
    Keywords: light ; temperature ; stolon growth medium ; below ground nodes ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five experiments studied the effects of shading, temperature and stolon growth medium on stolon and tuber formation. The lowest numbers of stolons and tubers were produced at the higher and lower nodes. Numbers of primary stolons and tubers were relatively stable across nodes and treatments and variation in total numbers was largely due to changes in numbers of lateral and branch stolons. In the field, 70% shading reduced numbers of stolons and tubers by reducing lateral and branch stolons, while in hydroponics, 45% shading and temperatures at initiation had no effect on tuber numbers. In both environments later planting tended to reduce numbers of stolons and tubers. There were large effects of the physical environment, with compost producing three times as many tubers as dry vermiculite. The potential number of tubers may be determined by the number of stolons and the environmental conditions affecting stolon formation and development.
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    Acta mathematicae applicatae sinica 13 (1997), S. 176-187 
    ISSN: 1618-3932
    Keywords: Spherical surface ; pseudospectral method ; vorticity equations ; stability ; convergence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract The pseudospectral method for solving vorticity equations on spherical surface is discussed. An interpolation procedure, which is different from the usual ones, is proposed. Based on such an interpolation, the pseudospectral scheme is constructed. Its generalized stability and convergence are analyzed rigorously. The theoretical analysis and computational skills can also be applied to other nonlinear partial differential equations defined on spherical surface.
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    Protoplasma 200 (1997), S. 154-162 
    ISSN: 1615-6102
    Keywords: Actin ; Cell elongation ; Confocal microscopy ; Zea mays ; Microfilaments ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In seedlings of maize (Zea mays L. cv. Percival), growth is controlled by the plant photoreceptor phytochrome. Whereas coleoptile growth is promoted by continuous far-red light, a dramatic block of mesocotyl elongation is observed. The response of the coleoptile is based entirely upon light-induced stimulation of cell elongation, whereas the response of the mesocotyl involves light-induced inhibition of cell elongation. The light response of actin microfilaments was followed over time in the epidermis by staining with fluorescence-labelled phalloidin. In contrast to the underlying tissue, epidermal cells are characterized by dense longitudinal bundles of microfilaments. These bundles become loosened during phases of rapid elongation (between 2–3 days in irradiated coleoptiles, between 5–6 days in dark-grown coleoptiles). The condensed bundles re-form when growth gradually ceases. The response of actin to light is fast. If etiolated mesocotyls are transferred to far-red light, condensation of microfilaments can be clearly seen 1 h after the onset of stimulation together with an almost complete block of mesocotyl elongation. The observations are discussed in relation to a possible role of actin microfilaments in the signal-dependent control of cell elongation.
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  • 46
    ISSN: 1615-6102
    Keywords: Auxin ; Blue light ; Cell growth ; Red light ; Mechanical stress ; Microtubule orientation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Changes in the orientation of cortical microtubules (longitudinal vs. transverse with respect to the long cell axis) at the outer epidermal wall of maize coleoptile segments were induced by auxin, red or blue light, and mechanical stresses (cell extension or compression produced by bending). Immunofluorescent techniques were used for the quantitative determination of frequency distributions of microtubule orientation. Detailed kinetic studies showed that microtubule reorientations are temporally correlated with the simultaneously measured changes in growth rate elicited by auxin, red light, or blue light. Growth inhibition induced by depletion of endogenous auxin produces a longitudinal microtubule pattern that can be changed into a transverse pattern in a dose-dependent manner by applying exogenous auxin. A mid-point pattern with equal frequencies of longitudinal and transverse microtubules was adjusted at 2 μmol/1 auxin. Bending stress applied under these conditions adjusts permanent, maximally longitudinal and transverse microtubule orientations at the compressed and extended segment sides, respectively, quantitatively mimicking the responses to differential flank growth during phototropic and gravitropic curvature. During tropic curvature the changes in microtubule pattern reflect the distribution of growth rather than the distribution of auxin. The microtubule pattern responds to auxin-dependent growth changes and mechanical stress in a synergistic manner, confirming the functional equivalence of these factors in affecting microtubule orientation. Similar results were obtained when segment growth was altered by blue or red light instead of auxin in the presence or absence of mechanical stress. It is concluded from these results that growth changes, elicited by auxin, light, etc., and mechanical stress affect microtubule orientation through a common signal perception and transduction chain.
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  • 47
    ISSN: 1615-6102
    Keywords: Arbuscular mycorrhiza ; Zea mays ; Hydroxyproline-rich glycoprotein ; In situ hybridization ; Glomus versiforme
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    Topics: Biology
    Notes: Summary To determine whether the expression of cell wall related genes changes during the establishment of an arbuscular mycorrhizal symbiosis (AM), we studied the expression of a maize hydroxyproline-rich glycoprotein (HRGP) gene. In situ hybridization showed that, in differentiated cells of maize roots, mRNA accumulation corresponding to the gene encoding for HRGP was only found when the cells were colonized by the endomycorrhizal fungusGlomus versiforme.
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    Protoplasma 198 (1997), S. 125-129 
    ISSN: 1615-6102
    Keywords: Glycoproteins ; Peroxidase-conjugated concanavalin A ; Plasma membrane ; Sperm cell ; Zea mays ; Indirect immune localization
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    Topics: Biology
    Notes: Summary After purifying plasma membranes from isolated maize sperm cells by aqueous polymer two-phase partition, peripheral and integral proteins were solubilized from the plasma membrane with Triton X-114 and separated in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Silver staining revealed 10 bands (19–68 kDa) of peripheral membrane proteins and about 40 bands (12–120 kDa) of integral proteins. Peroxidase-conjugated Con A was used to detect the surface glycopeptides. It was found that Con A particularly stained 8 peripheral polypeptide bands, including 68, 66, 55, 51,48, 44, 36, and 32 kDa, and 6 integral polypeptide bands, 68, 51, 48, 44, 38, and 34 kDa. These bands differed from those of somatic samples. Staining specificity was demonstrated by the control in the presence of competing inhibitory sugar. The above result indicates the existence of mannosyl and glucosyl residues in the surface glycoproteins of maize sperm cells. The prominent peripheral 68 kDa polypeptide was further separated into 4 spots by isoelectric focusing and sodium dodecyl sulfate two-dimensional (IEF-SDS 2-D) electrophoresis, showing pI values from 5.5 to 5.8. Three prominent glycopeptides (68, 48, and 32 kDa) were localized on the plasma membrane of maize sperm cells via the fluorescein isothiocyanate (FITC) technique. About 25% of sperm cells showed an intense positive reaction in each immunological labelling. The results agree with our previous labelling of the surface of isolated viable maize sperm cells with Con A-FITC.
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    Acta mechanica Sinica 13 (1997), S. 366-376 
    ISSN: 1614-3116
    Keywords: vibro-impact ; stability ; multiplicity
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The coexisting periodic impacting motions and their multiplicity of a kind of dual component systems under harmonic excitation are analytically derived. The stability condition of a periodic impacting motion is given by analyzing the propagation of small, arbitrary perturbation from that motion. In numerical simulations, the periodic impacting motions are classified according to the system states before and after an impact. The numerical results show that there exist many types of vibro-impacts and the bifurcation of periodic vibro-impacts is not smooth.
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    Numerical algorithms 14 (1997), S. 343-359 
    ISSN: 1572-9265
    Keywords: progressive interpolation ; stability ; spline ; shape parameters ; geometric continuity ; 41A05 ; 41A15 ; 65D05 ; 65D07
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    Topics: Computer Science , Mathematics
    Notes: Abstract In this paper, we study several interpolating and smoothing methods for data which are known “progressively”. The algorithms proposed are governed by recurrence relations and our principal goal is to study their stability. A recurrence relation will be said stable if the spectral radius of the associated matrix is less than one. The iteration matrices depend on shape parameters which come either from the connection at the knots, or from the nature of the interpolant between two knots. We obtain various stability domains. Moving the parameters inside these domains leads to interesting shape effects.
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    Biodiversity and conservation 6 (1997), S. 423-433 
    ISSN: 1572-9710
    Keywords: Biodiversity ; stability ; rain-forest ; logging ; fragmentation
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    Topics: Biology
    Notes: Abstract By comparing results from studies on the response of bird communities to selective logging and fragmentation of tropical moist forest and assessing whether different local faunas are differently affected, this paper examines whether communities in areas of unstable ecoclimatic histories may be more robust to change than those which evolved in places which were paleoecologically stable. Studies on selective logging in Asia and forest fragmentation in Latin America do not confidently demonstrate differences in the resilience of bird communities between stable and unstable areas. However, studies of selective logging and forest fragmentation in Africa give much stronger evidence for differences in fragility of local avifaunas, which correspond to what would be predicted from the paleoecological stability. Unfortunately, the currently available studies do not provide a basis for rigorous testing of the hypothesis. Comparison is constrained by lack of suitable controls, incomparable census methods, inadequate description of the disturbance regimes, and differences in the intensity of disturbance. It is suggested that well coordinated studies in many different areas, with good and standardized documentation of many habitat variables, may have considerable importance.
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    Mycoscience 38 (1997), S. 135-140 
    ISSN: 1618-2545
    Keywords: Aphanomyces frigidophilus ; morphology ; pH ; temperature
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    Topics: Biology
    Notes: Abstract Aphanomyces frigidophilus sp. nov. was obtained from eggs of Japanese char,Salvelinus leucomaenis, from Tochigi Prefectural Fisheries Experimental Station, Utsunomiya, Japan. Vegetative hyphae were delicate, slightly wavy, moderately branched. Zoosporangia were isodiametric with the vegetative hyphae. Oogonia were abundant, originating on short stalks from lateral sides of hyphae. Oogonia were spherical, subspherical or pyriform, with a single subcentric oospore inside. Outer surfaces of oogonia were roughened with short papillate, crenulate or irregular ornaments. Antheridia and oospore germination were not observed. Zoospore germination and vegetative growth were found from pH 5.0 to 11.0. Zoospore production was highest at 10°C, whereas rapid growth occurred at 20–25°C. Vegetative growth of the fungus declined from the maximal level at 25°C to less than half maximal at 30°C and completely disappeared at 35°C.
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    Acta mathematicae applicatae sinica 13 (1997), S. 33-44 
    ISSN: 1618-3932
    Keywords: Inverse problem ; hyperbolic equations ; eigenvalue problem ; spectral function ; integral kernel ; stability
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    Topics: Mathematics
    Notes: Abstract In this paper, the inverse boundary value problem of the hyperbolic system of first-order differential equations is discussed. The estimate of the solution and the quantitative analysis about its stability are obtained, and some stability criteria are established.
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  • 54
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    Journal of applied phycology 9 (1997), S. 269-276 
    ISSN: 1573-5176
    Keywords: Fucus distichus ; polar day ; trace metals ; light ; temperature ; tide ; rhythms
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    Topics: Biology
    Notes: Abstract Variation of Mn, Fe, Ni, Cu and Zn contents in thalli of Fucus distichus was determined under conditions of polar days. The use of spectrum analysis allowed estimates comparisons of the interrelation of periodic changes in irradiance, temperature and tidal fluctuations and quasiperiodic changes in metal contents. The effect of the diel rhythm of irradiance correlated with the accumulation of all metals except for Zn. At the same time, the changes in Mn, Fe and Cu content were delayed in respect to changes in irradiance level. The phase shift for Fe was 1 h 56 min, for Cu – 3 h 41 min and for Mn – 5 h5 min. The change in Ni content, in contrast, was 11min ahead of the change in irradiance. During the experiment an increase in water temperature up to10 °C resulted in increases in the average Mn, Ni and Cu contents in the thalli and decreases in Fe and Zn concents. The periodicity of metal thallus content did not always correspond to a diurnal rhythm, and couldbe longer (Mn, Ni: 32 h; Fe: 36 h) or shorter (Mn, Ni, Zn: 16 h; Cu: 12 h; Zn: 20, 10 h). The ANOVA of these rhythms allows an estimate of the contribution of rising and falling tidal fluctuations to the changes in Cu, Mn and Ni contents. There was a higher metal content during a falling than a rising tide. Abbreviations SDLT – semidiurnal lunartide, CDLT – closed diurnal lunar tide.
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  • 55
    ISSN: 1573-5176
    Keywords: Antarctic ; Arctic ; cyanobacteria ; green algae ; mass algal culture ; Phormidium ; temperature ; waste-water treatment
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    Topics: Biology
    Notes: Abstract Forty-nine strains of filamentous, mat-forming cyanobacteria isolated from the Arctic, subarctic and Antarctic environments were screened for their potential use in outdoor waste-water treatment systems designed for cold north-temperate climates. The most promising isolate (strain E18, Phormidium sp. from a high Arctic lake) grew well at low temperatures and formed aggregates (flocs) that could be readily harvested by sedimentation. We evaluated the growth and nutrient uptake abilities of E18 relative to a community of green algae (a Chlorococcalean assemblage, denoted Vc) sampled from a tertiary treatment system in Valcartier, Canada. E18 had superior growth rates below 15°C Canada. (µ = 0.20 d-1 at 10°C under continuous irradiance of 225 µmol photon m-2 s-1) and higher phosphate uptake rates below 10°C (k = 0.050 d-1 at 5°C) relative to Vc (µ=0.087 d-1 at 10°C and k = 0.020 d-1 at 5°C, respectively). The green algal assemblage generally performed better than E18 at high temperatures (at 25°C, µ = 0.39 d-1 and k = 0.34 d-1 for Vc; µ = 0.28 d-1 and k = 0.33 d-1 for E18). However, E18 removed nitrate more efficiently than Vc at most temperatures including 25°C. Polar cyanobacteria such as strain E18 are appropriate species for waste-water treatment in cold climates during spring and autumn. Under warmer summer conditions, fast-growing green algae such as the Vc assemblage are likely to colonize and dominate, but warm-water Phormidium isolates could be used at that time.
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  • 56
    ISSN: 1573-5176
    Keywords: Arthrospira (Spirulina) platensis ; integrated culture system ; raceway pond ; photobioreactor ; alveolar panel ; temperature
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    Notes: Abstract Cultivation of microalgae in closed photobioreactors suffers from high temperatures, whereas in open raceway ponds the optimal temperature is seldom reached. With an integrated device coupling a raceway pond with a suitably sized and positioned closed system (alveolar panel), the heat accumulated in the panel was efficiently transferred to the pond and a near-optimum temperature regimen for Arthrospira platensis was maintained, with no need for any additional cooling device. The productivity obtained in the integrated system was higher than the sum of the productivities (g reactor−1 day−1) of the pond and panel systems operating separately.
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  • 57
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    Journal of applied phycology 9 (1997), S. 229-236 
    ISSN: 1573-5176
    Keywords: nutrients ; growth rate ; Hydrodictyon reticulatum ; seasonal changes ; temperature ; growth prediction
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    Topics: Biology
    Notes: Abstract Since Hydrodictyon reticulatum was introduced to New Zealand it has spread rapidly and produced persistent annual nuisance growths in areas where nuisance algal had not occurred previously. Field bioassays were conducted at 10 sites between August 1993 and February 1995 to evaluate the seasonal growth patterns and the factors controlling growth under natural conditions. H. reticulatum exhibited a strong seasonal growth pattern with growth rates up to 0.33 doublings d-1 from August to March, are duction in growth rate in April and little or no growth from May to July. The H. reticulatum present in New Zealand has are latively low requirement for dissolved inorganic nitrogen (DIN) in comparison with other nuisance species, with its growth rate being saturated at 200 mg m-3. This and the high affinity for DIN as shown by a Ks of 29 mg m-3 have been key factors in the establishment of nuisance growths of H. reticulatum in New Zealand.
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    Journal of applied phycology 9 (1997), S. 451-457 
    ISSN: 1573-5176
    Keywords: Isochrysis galbana ; lipid ; biochemical composition ; fatty acid ; temperature ; growthphase
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    Topics: Biology
    Notes: Abstract The lipid and biochemical composition of the haptophyte Isochrysis galbana TK1 was examined. Cultures were grown at 15 °C and 30 °C, and harvested in the exponential and early stationary growth phases. Carbohydrate and protein content varied at the two culture temperatures and growth phases. The highest protein content was found at the exponential growth phase at 15 °C, and the highest carbohydrate content was found at the stationary phase at the same culture temperature. Lipid accumulated in the stationary growth phase and its content was higher at 30 °C than at 15 °C regardless of the growth phase. The neutral lipids were the major class of lipid found in all the cultures. The stationary phase culture had a higher proportion of neutral lipids than the exponential phase culture and the proportion decreased slightly when culture temperature was increased from 15 °C to 30 °C. Phospholipid levels remained constant at the two temperatures, but slightly decreased in the stationary phase. Glycolipids in the exponentially growing cells were higher than those from stationary growth phase and increased with temperature. Polyunsaturated fatty acids (PUFAs) predominated in glycolipids and phospholipids. Cells grown at 15 °C contained higher proportion of 18:3 (n–3) and 22:6 (n–3) with a corresponding decrease in 18:2 (n–6), monounsaturated and saturated fatty acids.
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  • 59
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    Aquatic ecology 31 (1997), S. 29-35 
    ISSN: 1573-5125
    Keywords: Daphnia ; microcosm ; three compartment microecosystem ; threshold food concentration ; stability ; state space
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    Topics: Biology
    Notes: Abstract A microecosystem consisting of three subsystems roughly representing the trophic levels of autotrophs, herbivores and decomposers was developed. A recirculating flow of water connected the three subsystems. Analysis of the data over a period of 7500 days indicates that the system has remained in the same state and has the prospect of being ‘immortal’. The Daphnia population showed periods of large oscillations, but also long periods of almost constant numbers. External factors were responsible for the initiation of the oscillations. The threshold food level for population increase was lower than mostly is found for individual growth, indicating adaptation to low food concentrations.
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  • 60
    ISSN: 1573-5125
    Keywords: toxicity ; cyanobacteria ; zooplankton ; population growth ; temperature
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    Topics: Biology
    Notes: Abstract The ability of cladocerans and rotifers to utilise the cyanobacterium Microcystis aeruginosa was tested by comparing the somatic and population growth in cultures using Chlorella and Microcystis as food types. Five species of cladocerans (Ceriodaphnia cornuta, Scapholeberis kingi, Moina macrocopa, Daphnia carinata, Simocephalus vetulus) and two species of rotifers (Brachionus calyciflorus, Hexarthra mira) were used in this study. In order to exclude the possibility of poor utilisation of Microcystis due to mechanical interference, single cells of Microcystis, (obtained by sonicating large colonies) were also offered. Experiments were done at 20 °Cs and 30 °C . In all the treatments tested, the population growth rate per day of the cladocerans ranged from -0.715 to 0.612 and that of the rotifers from -1.15 to 0.781. While C. cornuta, S. kingi and S. vetulus could utilise Microcystis, M. macrocopa and D. carinata were extremely susceptible to its toxins. The ability of the cladoceran populations to grow on Microcystis single cells was not related to the body length or gut length alone but to their ratio. The toxic effects of Microcystis were mitigated at the higher temperature. A strain of C. cornuta, collected from a Microcystis-dominated lake, had a higher growth rate on the toxic cyanobacteria suggesting that the tolerance to Microcystis could be a heritable trait. Of the two rotifer species, only H. mira survived and reproduced in some treatments of Microcystis.
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  • 61
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    Journal of applied phycology 9 (1997), S. 175-178 
    ISSN: 1573-5176
    Keywords: callus cells ; chloroplast ; differentiation ; Eckloniopsis radicosa ; Laminariales ; light intensity ; Phaeophyta ; temperature ; tissue culture
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    Topics: Biology
    Notes: Abstract Excised rectangular blade pieces of young sporophytes of Eckloniopsis radicosa (Kjellman) Okamura were cultured at four different temperatures (10, 15, 20, 25 °C) and light intensities (10, 20, 40, 80 µmol photon m−2 s−1) to observe callus cell propagation and differentiation. Although rapidly propagating callus cells were observed at a low temperature (15 °C) and low light intensities of (10–20 µmol photon m−2 s−1), differentiation to bladelets was only observed at higher temperatures and light intensities. In rapidly propagating callus cells, the chloroplasts decreased in both size and number. When callus cells with few chloroplasts were exposed to high temperature and light intensity, the chloroplasts gradually increased in number and size until they were the same size as found in cells from young blades. Finally, these cells differentiated and produced bladelets. This is the first report describing a relationship between differentiation and chloroplast size and number in the callus cells on E. radicosa.
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  • 62
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    Discrete event dynamic systems 7 (1997), S. 209-232 
    ISSN: 1573-7594
    Keywords: Stochastic recurrence equations ; performance evaluation ; ergodicity ; stability ; subadditive ergodic theory
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    Topics: Mathematics
    Notes: Abstract This paper deals with the asymptotic behavior of the stochastic dynamics of discrete event systems. In this paper we focus on a wide class of models arising in several fields and particularly in computer science. This class of models may be characterized by stochastic recurrence equations in ℝK of the form T(n+1) = φ n+1(T(n)) where φ n is a random operator monotone and 1—linear. We establish that the behaviour of the extremas of the process T(n) are linear. The results are an application of the sub-additive ergodic theorem of Kingman. We also give some stability properties of such sequences and a simple method of estimating the limit points.
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    Journal of scientific computing 12 (1997), S. 361-369 
    ISSN: 1573-7691
    Keywords: Alternating-direction implicit ; difference scheme ; stability ; convergence
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    Topics: Computer Science
    Notes: Abstract A new alternating-direction implicit (ADI) scheme for solving three-dimensional parabolic differential equations has been developed based on the idea of regularized difference scheme. It is unconditionally stable and second-order accurate. Further, it overcomes the drawback of the Douglas scheme and is to be very well to simulate fast transient phenomena and to efficiently capture steady state solutions of parabolic differential equations. Numerical example is illustrated.
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    Journal of scientific computing 12 (1997), S. 215-231 
    ISSN: 1573-7691
    Keywords: Transport models ; shallow water ; splitting methods ; stability
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    Topics: Computer Science
    Notes: Abstract We investigate the use of splitting methods for the numerical integration of three-dimensional transport-chemistry models. In particular, we investigate various possibilities for the time discretization that can take advantage of the parallelization and vectorization facilities offered by multi-processor vector computers. To suppress wiggles in the numerical solution, we use third-order, upwind-biased discretization of the advection terms, resulting in a five-point coupling in each direction. As an alternative to the usual splitting functions, such as co-ordinate splitting or operator splitting, we consider a splitting function that is based on a three-coloured hopscotch-type splitting in the horizontal direction, whereas full coupling is retained in the vertical direction. Advantages of this splitting function are the easy application of domain decomposition techniques and unconditional stability in the vertical, which is an important property for transport in shallow water. The splitting method is obtained by combining the hopscotch-type splitting function with various second-order splitting formulae from the literature. Although some of the resulting methods are highly accurate, their stability behaviour (due to horizontal advection) is quite poor. Therefore we also discuss several new splitting formulae with the aim to improve the stability characteristics. It turns out that this is possible indeed, but the price to pay is a reduction of the accuracy. Therefore, such methods are to be preferred if accuracy is less crucial than stability; such a situation is frequently encountered in solving transport problems. As part of the project TRUST (Transport and Reactions Unified by Splitting Techniques), preliminary versions of the schemes are implemented on the Cray C98 4256 computer and are available for benchmarking.
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    Journal of scientific computing 12 (1997), S. 353-360 
    ISSN: 1573-7691
    Keywords: Alternating-direction implicit ; difference scheme ; stability ; convergence
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    Topics: Computer Science
    Notes: Abstract A generalized Peaceman–Rachford alternating-direction implicit (ADI) scheme for solving two-dimensional parabolic differential equations has been developed based on the idea of regularized difference scheme. It is to be very well to simulate fast transient phenomena and to efficiently capture steady state solutions of parabolic differential equations. Numerical example is illustrated.
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    Journal of mathematical imaging and vision 7 (1997), S. 309-323 
    ISSN: 1573-7683
    Keywords: relaxation labeling processes ; consistency ; growth transformations ; Liapunov functions ; stability
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    Topics: Mathematics
    Notes: Abstract We present some new results which definitively explain thebehavior of the classical, heuristic nonlinear relaxation labelingalgorithm of Rosenfeld, Hummel, and Zucker in terms of theHummel-Zucker consistency theory and dynamical systems theory. Inparticular, it is shown that, when a certain symmetry condition is met,the algorithm possesses a Liapunov function which turns out to be (thenegative of) a well-known consistency measure. This follows almostimmediately from a powerful result of Baum and Eagon developed in thecontext of Markov chain theory. Moreover, it is seen that most of theessential dynamical properties of the algorithm are retained when thesymmetry restriction is relaxed. These properties are also shown tonaturally generalize to higher-order relaxation schemes. Someapplications and implications of the presented results are finallyoutlined.
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  • 67
    ISSN: 1573-8477
    Keywords: adaptive behaviour ; community dynamics ; functional response ; regulation ; stability
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    Topics: Biology
    Notes: Abstract A comparatively recent focus in consumer–resource theory has been the examination of whether adaptive foraging by consumers, manifested through the functional response, can stabilize consumer–resource dynamics. We offer a brief synthesis of progress on this body of theory and identify the conditions likely to lead to stability. We also fill a gap in our understanding by analysing the potential for adaptively foraging herbivores, which are constrained by time available to feed and digestive capacity, to stabilize dynamics in a single-herbivore/two-plant resource system. Because foraging parameters of the adaptive functional response scale allometrically with herbivore body size, we parameterized our model system using published foraging data for an insect, a small mammal and a large mammal spanning four orders of magnitude in body size, and examined numerically the potential for herbivores to stabilize the consumer–resource interactions. We found in general that the herbivore–plant equilibrium will be unstable for all biologically realistic herbivore population densities. The instability arose for two reasons. First, each herbivore exhibited destabilizing adaptive consumer functional responses (i.e. density-independent or inversely density-dependent) whenever they selected a mixed diet. Secondly, the numerical response of herbivores, based on our assumption of density-independent herbivore population growth, results in herbivores reaching densities that enable them to exploit their resource populations to extinction. Our results and those of studies we reviewed indicate that, in general, adaptive consumers are unlikely to stabilize the dynamics of consumer–resource systems solely through the functional response. The implications of this for future work on consumer–resource theory are discussed.
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  • 68
    ISSN: 1573-5117
    Keywords: sediments ; method ; organic matter ; difference-on-ignition ; weight ; temperature ; time
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For the routine determination of organic matter inmarine sediment, a rough estimate is often obtained bymeasuring the loss of weight on ignition(Difference-On-Ignition method: D.O.I.). This D.O.I. is to beused in ecological studies where organic material insediment is used as an environmental variable amongothers. A review of papers using this method showed agreat variability within conditions used. Theseconditions are generally used without accuratejustification. We propose a simple standard procedurebased on grain-size fraction of sediment to determineoptimal temperature and time of ignition, and theinfluence of the sample weight. The method proposed, using the loss of weight on ignition, will give thesame accuracy in measurements of organic matter inmarine sediments.
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  • 69
    ISSN: 1573-5117
    Keywords: microcrustacean zooplankton ; tropical limnology ; laboratory cultures ; growth ; development rates ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Length growth, instar durations, fecundity and mortality rates of fivespecies of microcrustacean zooplankton from a tropical reservoir weremeasured in relation to food quantity and temperature in laboratorycultures. Three cladocerans (Ceriodaphnia cornuta, Moina micrura,Diaphanosoma excisum), one calanoid copepod (Heliodiaptomus viduus), and onecyclopoid copepod (Mesocyclops thermocyclopoides) were studied. Filteredseston (45 µm mesh) from a local pond was used for food. Two foodconcentrations were employed: (1) 10 µg chlorophyll l−1(ca 0.25 mg Cl−1), and (2) 50 µg chlorophylll−1 (ca 1.25 mg C l−1). Food levels and watertemperature (22.5, 27.5, and 32.5 °C) used, roughly covered the rangesfound in the reservoir. Although all the three growth parameters were oftenaffected to some degree by temperature and food, the quantitative responseof the species differed. Also, the species reacted differently to the threepossible interactions (i.e. food × temperature,food × instar, andtemperature × instar). This contributed to the overalldifferences in the species responses. For the cladocerans, instar durationswere always affected by temperature. The food did not affect the durationtime of the adults and that of the combined juvenile instars, the latterexcept in one case significantly. For the two copepods food level affectedthe duration times of naupliar and copepodite instars, but the effect oftemperature was only significant for H. viduus. The development timesobserved for H. viduus were extraordinary long compared with values reportedin the literature for other tropical calanoids. This suggests that foodconditions in our culture were adversely affecting its growth rates.
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  • 70
    ISSN: 1573-5117
    Keywords: rotifers ; Brachionus plicatilis ; Brachionus rotundiformis ; energy content ; chemicalcomposition ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of temperature on the chemical composition (carbon, nitrogenand ash content) and the energy content of the rotifers Brachionusplicatilis and Brachionus rotundiformis (formerly B. plicatilis L- andS-type respectively) was examined in experimental populations. Dry weightand carbon, nitrogen and hydrogen content remained practically unchanged inthe 20–35 °C range in B. rotundiformis, while in B.plicatilis the dry weight decreased slightly with increasing temperaturefrom 15 ° to 35 °C. Nevertheless, themajor source of variation was the fecundity status of the populations. Onthe other hand, B. rotundiformis showed higher energy content (joules per mgof dry matter) than B. plicatilis. This difference in energy content was dueto the different ash content because the organic matter of both species hadthe same energy content, as well as the same basic biomass composition.
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    Hydrobiologia 364 (1997), S. 199-208 
    ISSN: 1573-5117
    Keywords: Phytoplankton ; photosynthesis ; light ; temperature ; tidal freshwater ; irradiance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The photosynthetic response to irradiance wasquantified for phytoplankton from the tidalfreshwater Potomac River biweekly to monthly over aperiod of six years. Samples were collected from twoshallow embayments and portions of the deeper rivermainstem. Photosynthetic rate was measured in thelaboratory at in situ temperature over a range ofirradiance levels and photosynthetic parameters werecalculated using nonlinear regression.PB max,the maximum photosynthetic ratestandardized to chlorophyll a, increased withtemperature up to 25 °C with a Q10 of 2.02. Above 25 °C, PB max was essentiallyconstant with temperature. Lesser correlationbetween PB max and ambient irradiance couldbe explained by the correlation of irradiance withtemperature. α, the slope of the P–I curve atlow light, was correlated with both ambientirradiance and temperature. Highest α valueswere found in late summer when high temperature andintermediate ambient irradiance were observed. Spring and early summer were characterized by lowα. Despite low light penetration, Ik andα values were indicative of sun limitationpossibly due to intermittent high light levelsexperienced during mixing. Ik showed a clearseasonal trend directly related to days from summersolstice. Spatial patterns were minimal except thatIk was consistently lower in one shallowembayment than in the other two areas. Seasonalpatterns in photosynthetic parameters correspondedroughly to changes from a spring diatom populationto summer cyanobacterial assemblage.
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  • 72
    ISSN: 1573-5028
    Keywords: cloning ; methyltransferase ; phytosterol ; yeast complementation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report the characterization of a higher-plant C-24 sterol methyltransferase by yeast complementation. A Zea mays endosperm expressed sequence tag (EST) was identified which, upon complete sequencing, showed 46% identity to the yeast C-24 methyltransferase gene (ERG6) and 75% and 37% amino acid identity to recently isolated higher-plant sterol methyltransferases from soybean and Arabidopsis, respectively. When placed under GAL4 regulation, the Z. mays cDNA functionally complemented the erg6 mutation, restoring ergosterol production and conferring resistance to cycloheximide. Complementation was both plasmid-dependent and galactose-inducible. The Z. mays cDNA clone contains an open reading frame encoding a 40 kDa protein containing motifs common to a large number of S-adenosyl-L-methionine methyltransferases (SMTs). Sequence comparisons and functional studies of the maize, soybean and Arabidopsis cDNAs indicates two types of C-24 SMTs exist in higher plants.
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  • 73
    ISSN: 1573-5028
    Keywords: cortex ; gene expression ; in situ hybridization ; organ-preferential ; root ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66 975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The function of the putative protein remains to be elucidated. The ZRP2 mRNA accumulates to the highest levels in young roots, and is also present in mature roots and stems of maize. Further analysis of young roots indicates that the lowest level of ZRP2 mRNA is near the root tip, with relatively high levels throughout the remainder of the root. In situ hybridization reveals that ZRP2 mRNA accumulates predominantely in the cortical parenchyma cells of the root. In vitro nuclear run-on transcription experiments indicate a dramatically higher level of zrp2 gene transcription in 3-day old roots than in 5-day old leaves. A zrp2 genomic clone, which includes the transcribed region and 4.7 kb of upstream sequence, was isolated and characterized.
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  • 74
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    European journal of plant pathology 103 (1997), S. 573-580 
    ISSN: 1573-8469
    Keywords: Pisum sativum ; host plant exudates ; light ; temperature ; infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of host plant exudates, light and temperature on germination of oospores of Peronospora viciae f.sp. pisi in vitro were investigated. Seed and root exudates did not increase percentage germination, whereas light inhibited germination. The first germ tubes appeared after 4, 7, and 14 days of incubation at 15, 10 and 5 °C, respectively. The eventual level of germination was highest and had similar values at 5 and 10 °C. At 20 °C germination was poor and at 25 °C no germination was observed. Oospores placed on membrane filters were incubated on soil. When oospores were retrieved from the membrane filters after six days and placed in water at 10 °C, they germinated within 2 days. On soil significantly less oospores germinated than in water. Germinability of oospores stored in the dark at 5 or 20 °C at 30 or 76% RH was studied over a two-year period. Germinability generally increased over time, but fluctuations were observed indicating the occurrence of secondary dormancy. Time courses of germinability were generally similar for oospores stored at several temperatures and humidities. No effect of light on time course of germinability was found when oospores were exposed to alternating light-dark periods or stored in continuous dark for 140 days. Percentage germination observed in a germination assay was correlated with percentage infection determined in a bioassay.
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  • 75
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    Journal of dynamical and control systems 3 (1997), S. 291-319 
    ISSN: 1573-8698
    Keywords: 34D15 ; 34E15 ; 34A60 ; 93C73 ; 93D20 ; 49N99 ; Singular perturbation ; differential inclusion ; control systems ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract We study the continuity properties of the bundle of solutions to a differential inclusion subject to a singular perturbation, i.e., with respect to a scalar parameter ɛ multiplying a part of the derivatives. We give conditions under which every solution of the singularly perturbed inclusion is close, in a certain sense and for a sufficiently small ɛ, to a solution of the degenerate inclusion obtained for ɛ=0. These conditions include both stability and structural requirements (the later having no counterpart in the case of a differential equation). The main result obtained generalizes the well-known Tikhonov theorem for singularly perturbed differential equations.
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  • 76
    ISSN: 1573-904X
    Keywords: growth hormone ; stability ; poly(lactic-co-glycolic acid) ; microencapsulation ; degradation ; sustained release
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. The development of a sustained release formulation for recombinant human growth hormone (rhGH) as well as other proteins requires that the protein be stable at physiological conditions during its in vivo lifetime. Poly(lactic-co-glycolic acid) (PLGA) microspheres may provide an excellent sustained release formulation for proteins, if protein stability can be maintained. Methods. rhGH was encapsulated in PLGA microspheres using a double emulsion process. Protein released from the microspheres was assessed by several chromatrographic assays, circular dichroism, and a cell-based bioassay. The rates of aggregation, oxidation, diketopiperazine formation, and deamidation were then determined for rhGH released from PLGA microspheres and rhGH in solution (control) during incubation in isotonic buffer, pH 7.4 and 37°C. Results. rhGH PLGA formulations were produced with a low initial burst (〈20%) and a continuous release of rhGH for 30 days. rhGH was released initially from PLGA microspheres in its native form as measured by several assays. In isotonic buffer, pH 7.4 and 37°C, the rates of rhGH oxidation, diketopiperazine formation, and deamidation in the PLGA microspheres were equivalent to the rhGH in solution, but aggregation (dimer formation) occured at a slightly faster rate for protein released from the PLGA microspheres. This difference in aggregation rate was likely due to the high protein concentration used in the encapsulation process. The rhGH released was biologically active throughout the incubation at these conditions which are equivalent to physiological ionic strength and pH. Conclusions. rhGH was successfully encapsulated and released in its fully bioactive form from PLGA microspheres over 30 days. The chemical degradation rates of rhGH were not affected by the PLGA microspheres, indicating that the internal environment of the microspheres was similar to the bulk solution. After administration, the microspheres should become fully hydrated in the subcutaneous space and should experience similar isotonic conditions and pH. Therefore, if a protein formulation provides stability in isotonic buffer, pH 7.4 and 37°C, it should allow for a safe and efficacious sustained release dosage form in PLGA microspheres.
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  • 77
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    Pharmaceutical research 14 (1997), S. 606-612 
    ISSN: 1573-904X
    Keywords: hIGF-I ; benzyl alcohol ; preferential interaction ; stability ; preservative
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. The solubility and physical stability of human Insulin-like Growth Factor I (hIGF-I) were studied in aqueous solutions with different excipients. Methods. The solubility of hIGF-I was determined by UV-absorption and quantification of light blocking particles. The physical stability of hIGF-I was studied with differential scanning calorimetry (DSC) and circular dichroism (CD) spectroscopy. Results. Human IGF-I precipitated at low temperature in the presence of 140 mM benzyl alcohol and 145 mM sodium chloride. CD data showed that the tertiary structure of hIGF-I during these conditions was perturbed compared to that in 5 mM phosphate buffer. In the presence of benzyl alcohol 290 mM mannitol stabilized hIGF-I. Sodium chloride or mannitol by themselves had no effect on either the solubility or the tertiary structure. Benzyl alcohol was attracted to hIGF-I, whereas sodium chloride was preferentially excluded. The attraction of benzyl alcohol was reinforced by sodium chloride leading to salting-out of hIGF-I. The CD-data indicated interactions of benzyl alcohol with phenylalanine in hIGF-I. Thermal denaturation of hIGF-I occurred in all solutions with sodium chloride, whereas mannitol or benzyl alcohol had no effect on the thermal stability. The thermal stability of hlGF-I was thus decreased in 145 mM sodium chloride although it was excluded from hIGF-I. Conclusions. The self-association and thermal aggregation of hIGF-I is driven by hydrophobic interactions. Benzyl alcohol is attracted to hIGF-I and induces changes in the tertiary structure causing hydrophobic attraction of the protein at low temperatures.
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  • 78
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    Journal of economics 66 (1997), S. 1-22 
    ISSN: 1617-7134
    Keywords: diffusion of innovations ; Walrasian equilibrium ; stability ; O31
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract When all products in the economy are weak gross substitutes, preferences are homothetic, and firms face menu costs then all prices in an industry move together at the same rate. In the closed-loop Nash noncooperative equilibrium, all firms invest in productivity and reduce real prices. As a result, in the case of quadratic menu costs, the outputs of industries and the economy go up along S-shaped time paths characteristic of diffusion of innovations.
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  • 79
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    Journal of economics 66 (1997), S. 271-282 
    ISSN: 1617-7134
    Keywords: Stackelberg model ; dynamic oligopoly ; stability ; C62 ; C73 ; D43
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract We investigate the (dynamic) stability of a stackelberg oligopoly model of a market of a homogeneous good, with output competition, one Stackelberg leader and a number of identical followers. We assume that each firm incurs quadratic production-adjustment costs if it changes its output. We present a simple necessary and sufficient condition for stability of the model. Using the condition, we compare the stability of this model with the stability of two related Cournot models in which all firms present are followers. It turns out that the Stackelberg model is “more stable” than these two Cournot models.
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  • 80
    ISSN: 1617-4623
    Keywords: Key words Ac Transposable element ; Zea mays ; DNA binding domain ; Gel retardation assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The mobility of maize transposable element Activator (Ac) is dependent on the 11-bp terminal inverted repeats (IRs) and approximately 250 subterminal nucleotides at each end. These sequences flank the coding region for the transposase (TPase) protein, which is required for the transposition reaction. Here we show that Ac TPase has a bipartite DNA binding domain, and recognizes the IRs and subterminal sequences in the Ac ends. TPase binds cooperatively to repetitive ACG and TCG sequences, of which 25 copies are found in the 5′ and 20 copies in the 3′ subterminal regions. TPase affinity is highest when these sites are flanked on the 3′ side by an additional G residue (A/TCGG), which is found at 75% of binding sites. Moreover, TPase binds specifically to the Ac IRs, albeit with much lower affinity. Two mutations within the IRs that immobilize Ac abolish TPase binding completely. The basic DNA binding domain of TPase is split into two subdomains. Binding to the subterminal motifs is accomplished by the C-terminal subdomain alone, whereas recognition of the IRs requires the N-terminal subdomain in addition. Furthermore, TPase is extremely flexible in DNA binding. Two direct or inverted binding sites are bound equally well, and sites that are five to twelve bases apart are similarly well bound. The consequences of these findings for the Ac transposition reaction are discussed.
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  • 81
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    Molecular genetics and genomics 256 (1997), S. 158-168 
    ISSN: 1617-4623
    Keywords: Key words Transposon ; Ac ; Regulation ; Zea mays ; Excision
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plants form their gametes late in somatic development and, as a result, often pass somatic mutations on to their progeny. Classic examples of this process are the germinal revertants of unstable, Ac/Ds transposon-induced kernel mutations in maize: frequent and early reversion events during somatic development are generally correlated with a high frequency of revertant gametes. We have characterized a Ds allele of the maize waxy(wx) gene, wx-m5:CS7, for which the correlation between somatic and germinal reversion frequencies no longer holds. The ability of wx-m5:CS7 (CS7) to produce revertant gametes is suppressed ∼100-fold in comparison with a second Ds allele, wx-m5:CS8 (CS8), which has an identical insertion at Wx and the same frequent and early somatic reversion pattern in endosperm. The excision of Ds from wx is not reduced 100-fold in the somatic tissues of CS7 plants as compared with CS8 plants. Suppressed formation of CS7 revertant gametes is independent of the Ac transposase source and is heritably passed to the embryos of progeny kernels; however, frequent and early somatic reversion is observed again in endosperms of these progeny kernels. This suppression appears to be caused by a dominant mutation in a trans-acting product that can suppress the germinal reversion of other Ds-induced alleles as well; the mutation is tightly linked to Wx but is not in the CS7 Ds itself. Taken together, the data suggest a novel mode of developmental control of Ac/Ds elements by the host plant, suppressing element excision in the shoot meristem.
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  • 82
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    Colloid & polymer science 275 (1997), S. 467-473 
    ISSN: 1435-1536
    Keywords: Key words Block copolymers ; micelle ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  The association behaviour of triblock copoly(ethylene oxide/tetrahydrofuran/ethylene oxide), in particular E100T27E100, in aqueous solutions has been investigated by means of static and dynamic light scattering, nuclear magnetic reso-nance (NMR) and surface tension techniques. On raising the polymer concentration at room temperature, the copolymer aggregates to form micelles with an aggregation number of about 105 (R G, mic≈15 nm and R H, mic≈13 nm, as revealed by light scattering and FT-PGSE NMR measurements, respectively). The micelles are kinetically quite stable, the micellar lifetime is shown to be more than 1 h. The residence time of a single unimer in a micelle is more than 140 ms. The apparent radius of gyration R G, mic is fairly independent of concentration, but large effects are observed on varying the temperature. Raising the temperature initially results in an increase of the apparent micellar size, followed by a maximum at an intermediate temperature (≈45 °C). At higher temperatures a contraction of the micelles is observed. The shape of the micelles also appear to vary in this temperature interval. The interactions responsible for these phenomena are discussed in terms of, e.g., the temperature-dependent solubility of the alkylene oxide segments in water and polydispersity effects.
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  • 83
    ISSN: 1573-5060
    Keywords: leafy normal-stature ; leafy reduced-stature ; non-leafy normal-stature ; non-leafy reduced-stature ; maize ; short season environments ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Development of maize (Zea mays L.) types that produce leaf area and mature quickly would increase production of maize in mid- to short-season areas. The leafy (Lfy1) and reduced-stature (rd1) traits both make contributions to this end. However, these two traits have not previously been combined. Our objective was to evaluate the yield and yield components of non-leafy normal-stature (NLNS), leafy reduced-stature (LRS), non-leafy reduced-stature (NLRS), and leafy normal-stature (LNS) maize inbred lines. The two genes, ‘Lfy1’ and ‘rd1’, were incorporated into a series of inbred lines resulting in a range of canopy architectures. Ten variables were recorded for each of 30 inbred lines over three years. The 10 variables were: corn heat unit requirement from planting to tasselling, corn heat unit requirement from planting to silking, days between tasselling and silking, grain moisture content, husk dry weight, cob dry weight, ear length, maximum ear circumference, grain yield and ratio of grain yield to moisture content. Reduced-stature inbred lines reached anthesis more quickly than normal-stature inbred lines. Grain moisture content was less in reduced-stature inbred lines than normal stature trait groups. Leafy-reduced stature plants had the highest ratio of grain to moisture content and the lowest grain moisture content at harvest. Inbred lines containing the rd1 trait matured more rapidly than other trait groups. The LRS trait group yielded more than the other groups, and showed great potential for use in mid- to short-season environments.
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  • 84
    ISSN: 1573-5060
    Keywords: water stress ; breeding ; adaptation ; G × E ; clustering ; ordination ; Zea mays ; corn ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ten trials evaluated the performance of several late tropical maize populations (La Posta Sequía, Pool 26 Sequía and Tuxpeño Sequía) selected for tolerance to drought during flowering and grain filling and also for yield potential. Families (S1 or full-sib) had been selected recurrently for six to eight years on an index of traits. Pattern (clustering and ordination) analysis was used to analyse the relative performance of entries that included cycles of selection for drought tolerance in the populations and non-drought tolerant checks. Mean environment (E) yields ranged from 1.0 to 10.4 t ha-1. Analysis of variance showed that 97.9% of the total sums of squares was accounted for by E, and that, of the remaining sums of squares the G × E (genotype by environment interaction) was almost 3 times that of the contribution of G alone. Cluster analysis separated the checks, the earlier maturing drought tolerant entries and the later maturing drought tolerant entries. This was verified by principal component (PC) analysis of the G × E matrix. Grouping of the environments (i.e. based on entry performance), resulted in the separation of different types of droughts, and of medium and high yielding well-watered environments. The patterns of discrimination observed indicated that the yield gains under drought would have been unlikely to occur if selection had been done only in well-watered environments. Within each population, selection improved broad adaptation (higher mean yield) to both drought and well-watered environments and cycles of selection ‘jumped’ from non-drought-tolerant to drought-tolerant groups as their specific adaptation to drought environments increased.
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  • 85
    ISSN: 1573-5036
    Keywords: catch crop ; mineralisation ; nitrogen ; simulation model ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The release of nitrogen from incorporated catch crop material in winter is strongly influenced by soil temperatures. A laboratory experiment was carried out to investigate this influence in the range of 1-15 °C. Samples of sandy soil or a mixture of sandy soil with rye shoots were incubated at 1-5-10-15 °C, and samples of sandy soil with rye roots were incubated at 5-10-15 °C. Concentrations of Nmin (NH4 +-N and NO3 --N) were measured after 0-1-2-4-7-10 weeks for the sandy soil and the sandy soil:rye shoot mixture, and after 0-2-7-10 weeks for the sandy soil:rye root mixture. At 1 °C, 20% of total organic N in the crop material had been mineralised after ten weeks, indicating that mineralisation at low temperatures is not negligible. Maximum mineralisation occurred at 15 °C; after ten weeks, it was 39% of total applied organic nitrogen from shoot and 35% from root material. The time course of mineralisation was calculated using an exponential decay function. It was found that the influence of temperature in the range 1-15 °C could be described by the Arrhenius equation, stating a linear increase of ln(k) with T-1, k being the relative mineralisation rate in day-1 and T the temperature (°C). A simulation model was developed in which decomposition, mineralisation and nitrification were modelled as one step processes, following first order kinetics. The relative decomposition rate was influenced by soil temperature and soil moisture content, and the mineralisation of N was calculated from the decomposition of C, the C to N ratio of the catch crop material and the C to N ratio of the microbial biomass. The model was validated first with the results of the experiment. The model was further validated with the results of an independent field experiment, with temperatures fluctuating between 3 and 20 °C. The simulated time course of mineralisation differed significantly from the experimental values, due to an underestimation of the mineralisation during the first weeks of incubation.
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  • 86
    ISSN: 1573-5036
    Keywords: enzymatic isolation ; K+ channel ; patch clamp ; xylem contact cells ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Because of their importance for nutrition, a method was developed to patch xylem contact cells in leaves of Vicia faba and maize. Since the lignification of older cells was a major obstacle for isolating protoplasts which could be patched, only young leaves (fourth fully developed leaf) were used. An important step in the isolation of these cells was the infiltration of the leaves and their exposure to enzymes for several hours, allowing mesophyll cells to be removed whilst having most of the xylem contact cells attached to the xylem. Channel activity in cell-attached mode or in excised patches could only be observed if an internal coating of sigmacote was used to block diffusion ions out of the pipette glass. Two different types of K+ channels were identified by measuring the reversal potential at different concentrations of KCl. One channel (SC) had a symmetric IV curve with a high probability of remaining open, irrespective of membrane potential; the other channel was an inward rectifier. The symmetical channel could be blocked weakly by Na+ but it was permeable to $${\text{NH}}_{{\text{4}}^{\text{ + }} } $$ .
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  • 87
    ISSN: 1573-5036
    Keywords: carbohydrates ; carbon dioxide ; nitrogen ; nutrients ; roots ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Rising atmospheric carbon dioxide, nitrogen deposition and warmer temperatures may alter the quantity and quality of plant-derived organic matter available to soil biota, potentially altering rates of belowground herbivory and decomposition. Our objective was to simulate future growth conditions for an early successional (loblolly) and late successional (ponderosa) species of pine to determine if the physical and chemical properties of the root systems would change. Seedlings were grown for 160 days in greenhouses at the Duke University Phytotron at 35 or 70 Pa CO2 partial pressure, ambient or ambient + 5 °C temperature, and 1 or 5 mMNH4O3. Roots from harvested seedlings were analyzed for changes in surface area, specific root length, mass, total nonstructural carbohydrates (TNC), and concentrations of macro-nutrients. Surface area increased in both species under elevated CO2, due primarily to increases in root length, and this response was greatest (+138%) in loblolly pine at high temperature. Specific root length decreased in loblolly pine at elevated CO2 but increases in mass more than compensated for this, resulting in net increases in total length. TNC was unaffected and nutrient concentrations decreased only slightly at elevated CO2, possibly from anatomical changes to the root tissues. We conclude that future growth conditions will enhance soil exploration by some species of pine, but root carbohydrate levels and nutrient concentrations will not be greatly affected, leaving rates of root herbivory and decomposition unaltered.
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  • 88
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    Plant and soil 190 (1997), S. 87-90 
    ISSN: 1573-5036
    Keywords: minirhizotron ; root longevity ; root mortality ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The production and mortality of roots is an important factor determining nutrient and carbon fluxes in terrestrial ecosystems. However, the effect of temperature on the longevity of roots is not understood. In this study the impact of changes in temperature on the longevity of Lolium perenne roots was determined. Plants were grown at 15 °C, 21 °C and 27 °C and their roots observed using a minirhizotron system. Major effects of temperature were measured with large reductions in root longevity occurring at higher temperatures. The implications of these data are discussed.
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    Plant and soil 192 (1997), S. 23-30 
    ISSN: 1573-5036
    Keywords: aluminium ; callose ; protoplasts ; resistance ; toxicity ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The screening of 37 Zea mays L. cultivars in nutrient solution using root elongation (24 h) as a parameter showed large genotypic differences in Al resistance among the genetic material evaluated. Callose concentrations in root tips were closely and positively related to Al-induced inhibition of root elongation. Therefore, Al-induced callose formation in root tips appears to be an excellent indicator of Al injury and can be used as a selection criteria for Al sensitivity. In contrast, aluminium concentrations in root tips were not related to Al-induced inhibition of root elongation, nor to Al-induced callose formation. Callose formation was also induced by short-term A1 treatment in root tip protoplasts, and the response of protoplasts clearly reflected the cultivar-specific response to Al of intact roots. This indicates that in maize, Al sensitivity is expressed on the protoplast level.
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  • 90
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    Plant cell, tissue and organ culture 51 (1997), S. 49-56 
    ISSN: 1573-5044
    Keywords: CO2 enrichment ; gas exchange ; in vitro environment ; light ; photoautotrophic micropropagation ; relative humidity ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Leafy or chlorophyllous explants of a number of plant species currently micropropagated have been found to have high photosynthetic ability. Their growth and development have been promoted on sugar-free medium rather than on sugar-containing medium, provided that the environmental factors, such as CO2 concentration, light intensity and relative humidity, are controlled for promoting photosynthesis and transpiration of explants/shoots/plantlets in vitro. Thus, environmental control is essential for promoting photosynthetic growth and development of in vitro plantlets. Several types of sugar-free (photoautotrophic) culture systems for large-scale micropropagation of plants have been developed. Advantages of sugar-free over conventional (heterotrophic or photomixotrophic) micropropagation systems are as follows: growth and development of plantlets in vitro are faster and more uniform, plantlets in vitro have less physiological and morphological disorders, biological contamination in vitro is less, plantlets have a higher percentage of survival during acclimatization ex vitro, and larger culture vessels could be used because of less biological contamination. Hence, production costs could be reduced and plant quality could be improved significantly with photoautotrophic micropropagation. Methods for the measurement and control of in vitro environments and the beneficial effects of environmental control on photosynthetic growth, development, and morphogenesis in large-scale production of micropropagated plantlets are presented.
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  • 91
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    Euphytica 94 (1997), S. 53-62 
    ISSN: 1573-5060
    Keywords: adaptation ; AMMI ; cereals ; genotype-environment interaction ; joint regression ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Joint regression and Additive Main effects and Multiplicative Interaction (AMMI) models were compared for i) capacity of describing genotype-location (GL) and genotype-environment (GE) interaction effects (environments = location-season combinations), assessed in terms of estimated variance of heterogeneity of genotype regressions and of the sum of the variances of significant interaction principal component (PC) axes, and ii) repeatability between cropping seasons of measures of genotype stability across locations. These measures were Finlay and Wilkinson's regression coefficient for joint regression, and the Euclidean distance from the origin of significant interaction PC axes (D) and the absolute value of PC 1 score (| PC 1 |) for AMMI. Shukla's stability variance (σsup2;) was considered in addition. The study included three data sets for durum wheat, two for maize and one each for bread wheat and oat. Relationships between climatic variables and GL interaction occurrence were also assessed. AMMI proved distinctly more valuable in six data sets for description of GE effects and in four for description of GL effects over seasons. Its superiority was not crop-specific and tended to occur when more, distinct environmental constraints affected genotype responses. When both methods were appropriate, they provided a similar ordination of sites and genotypes for GL effects. The models that adequately described GL interaction over seasons generally provided also stability measures that were moderately repeatable between seasons. D was better repeatable than | PC 1 | and σ& 2; in a few cases. Ordination of locations on GL interaction PC 1 tended to be consistent both between wheat and between maize data sets having either no seasons or no genotypes in common.
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  • 92
    ISSN: 1573-5060
    Keywords: water stress ; breeding ; ordination ; clustering ; three-way analysis ; Zea mays ; corn
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A selection program in three tropical maize populations aimed to improve tolerance of mid-season to late season drought environments while maintaining grain yield (GY) potential. The selection process employed other attributes that included maintaining a constant anthesis date (AD) and, under drought, shortening the anthesis-silking interval (ASI) and increasing ear number per plant (EPP). Three-mode (genotypes × environments × attributes) pattern analysis, which consists of clustering and ordination, should be able to collectively interpret these changes from ten evaluation trials. Mixture maximum likelihood clustering identified four groups that indicated the populations' performance had changed with selection. Groups containing the advanced cycles of selection were higher yielding in most environments and had lower ASI and higher EPP, particularly in drought environments. Check entries with no selection for drought tolerance remained grouped with the initial cycles of selection. A 3 × 2 × 3 (genotypes by environments by attributes) principal component model explained 70% of the variation. For the first environmental component, ASI was shown to be highly negatively correlated with both GY and EPP while anthesis date (AD) was virtually uncorrelated with other traits. The second environmental component (explaining 10% of the variation) contrasted droughted and well-watered environments and showed that EPP and GY were better indicators of this contrast (in terms of changes in population performance) than were AD or ASI. Three-mode analysis demonstrated that improvements with selection occurred in both droughted and well-watered environments and clearly summarised the overall success of the breeding program.
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  • 93
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    Plant growth regulation 21 (1997), S. 183-187 
    ISSN: 1573-5087
    Keywords: growth elongation ; nitric oxide ; No·–releasing substances ; IAA ; nitrite ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Root segments of maize were incubated in different solutions containing substances that non-enzymatically release nitric oxide, such as sodium nitrite (SN), sodium nitroprusside (SNP), nitrosoglutathione (NGLU) and nitrosocysteine (NCYS). We found that all of these substances induced root tip expansion in a dose-dependent manner. The decreasing order of potency for root-induced elongation was: 10 -7 M SN, pH 4.5; 10 -11 M NCYS, 10 -10 M SNP, 10 -9 M NGLU and 10 -7 M SN, pH 7.0. Nitric oxide scavenger such as methylene blue prevented the elongation induced by NO·–releasing substances, but had no effect on indole-3-acetic acid (IAA)-induced cell expansion. Our results suggest that nitric oxide is the putative elongation inducer and that IAA and NO·–releasing substances conceivably share common steps in the signal transduction pathway, since both elicited the same plant response. Vanadate, a plasmamembrane ATPase inhibitor, significantly reversed IAA-induced elongation when supplied at 10 μM concentration. IAA-induced elongation was strongly enhanced by 10 nM BAY K 8644, an agonist of voltage dependent Ca2+ channels. Promotion of root elongation in the absence of IAA occurred only at higher concentrations of BAY K. Vanadate and BAY K had no influence on the NCYS-induced elongation suggesting that the common steps in the signalling of IAA and NCYS are not at the level of the plasmamembrane.
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  • 94
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    Plant growth regulation 21 (1997), S. 215-221 
    ISSN: 1573-5087
    Keywords: Auxin analogues ; bound auxins ; duckweed ; Lemna gibba ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two strategies for selection of plant mutants altered in their ability to hydrolyze IAA conjugates are described. The rationale for both strategies is to form a conjugate between two chemical moieties in which one moiety is a toxin. The first strategy makes use of a toxic auxin analogue. For this approach a series of halogenated IAA analogues were tested for toxic properties to Lemna gibba and Zea mays. Several halogenated analogues were toxic, but only 5-Br-IAA was highly toxic to both species. The second strategy required a toxic compound with suitable functional groups to allow IAA conjugation. The mycotoxin patulin was found to be a suitable candidate. Uptake and hydrolysis of IAA-conjugates was studied in order to better define the conditions required for conjugate analogues to be effectively employed for selections.
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  • 95
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    Fish physiology and biochemistry 16 (1997), S. 381-393 
    ISSN: 1573-5168
    Keywords: pike ; eggs ; larvae ; embryonic development ; fatty acid ; lipid class ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To establish the changes which occur during embryogenesis and early larval development, eggs, yolk sac larvae and swim-up larvae of pike were examined for lipid class and fatty acid composition. At a water temperature of 15.5°C, the embryonic phase was short (6 days) and characterized by a 41.3% decline in the lipid content of eggs, accompanied by large reductions in the amount of phosphatidylcholine (41.4% decrease), sterol esters and triacylglycerols (respectively a 41.2% decrease and a 58.1% decrease), but not phosphatidylethanolamine which increased markedly (35.6%). By the time of yolk sac absorption (7 to 11 days after fertilization) the larvae remained inactive and a limited utilization of lipids was observed. Yolk sac phosphatidylcholine was selectively incorporated into larval bodies while the levels of other lipid classes remained unchanged in the yolk. When the swim bladder was filled and the swimming stage was reached (11 days to 13 days af), the yolk was completely depleted and yolk phosphatidylcholine together with yolk triacylglycerols were catabolised. Yolk phosphatidylethanolamine and yolk sterol esters were partly incorporated into the body lipids. In the subsequent swim-up larval stage (13 to 15 days af), a steady decrease in lipids was observed (41.6%). Fluctuations in the levels of polyunsaturated fatty acids, monounsaturated fatty acids or saturated fatty acids examined from eggs or larvae were consistent with changes in lipid classes during pike development. During yolk sac absorption, pike incorporated yolk PUFA released on hydrolysis of phosphatidylcholine into the larval body. The results are discussed with reference to water temperature and in relation to the ontogenic and ecological context of pike development.
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  • 96
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    Fish physiology and biochemistry 16 (1997), S. 355-364 
    ISSN: 1573-5168
    Keywords: Na-K-ATPase ; pNPPase ; channel arrest ; fish heart ; thermal acclimation ; anoxia ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of seasonal acclimatization, thermal acclimation and anoxic exposure on total cation-activated ATPase and sarcolemmal Na-K-ATPase activities of crucian carp heart were measured. The total cation-activated ATPase showed a positive correlation with environmental temperature: activity was highest in mid summer and lower in early spring and late autumn. The decline of total ATPase activity during cold seasons suggests that energy consumption of the heart is reduced during the anoxic winter period by depression of ATP utilization rate. In contrast to the total ATPase activity, the activity of sarcolemmal Na-K-ATPase did not correlate strongly with environmental temperature but remained relatively constan during most of the study period. However, in late autumn when oxygen content of water is low, a prominent decline in Na-K-ATPase activity was noted. The latter finding suggests that the activity of Na-K-pump of the cardiac sarcolemma is unaffected by seasonal temperature changes but is depressed by hypoxic exposure. In accordance with this short-term anoxia (100 h) in laboratory conditions reduced Na-K-ATPase or pNPPase activities of cardiac homogenates while long-term thermal acclimation (〉4 weeks) was ineffective in this respect. The present findings suggest that cation-activated ATPases of crucian carp heart are downregulated during winter, and part of this reduction is due to anoxic depression of sarcolemmal Na-K-ATPase.
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  • 97
    ISSN: 1573-515X
    Keywords: acidification ; aluminium ; Arrhenius’ law ; calcium ; cation leaching ; climate ; ion equilibrium ; forest soil ; N-cycle ; N-deposition ; nitrification ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Increased emissions of nitrogen compounds have led to atmosphericdeposition to forest soils exceeding critical loads of N overlarge parts of Europe. To determine whether the chemistry offorest soils responds to changes in throughfall chemistry, intactsoil columns were reciprocally transplanted between sites, withdifferent physical conditions, across a gradient of N and Sdeposition in Europe. The transfer of a single soil to the various sites affected itsnet nitrification. This was not simply due to the nitrificationof different levels of N deposition but was explained bydifferences in physical climates which influenced mineralizationrates. Variation in the amount of net nitrification between soiltypes at a specific site were explained largely by soil pH. Within a site all soil types showed similar trends in netnitrification over time. Seasonal changes in net nitrificationcorresponds to oscillations in temperature but variable time lagshad to be introduced to explain the relationships. WithArrhenius‘ law it was possible to approximate gross nitrificationas a function of temperature. Gross nitrification equalled netnitrification after adaptation of the microbial community oftransplanted soils to the new conditions. Time lags, andunderestimates of gross nitrification in autumn, were assumed tobe the result of increased NH 4 + availability due either tochanges in the relative rates of gross and net N transformationsor to altered soil fauna-microbial interactions combined withimproved moisture conditions. Losses of NO 3 - were associated with Ca2+and Mg2+ in non-acidified soil types and with losses ofAl3+ in the acidified soils. For single soils the ionequilibrium equation of Gaines-Thomas provided a useful approximationof Al3+ concentrations in the soil solution as a functionof the concentration of Ca2+. The between site deviationsfrom this predicted equilibrium, which existed for single soils, couldbe explained by differences in throughfall chemistry which affectedthe total ionic strength of the soil solution. The approach of reciprocally transferring soil columnshighlighted the importance of throughfall chemistry, interactingwith the effect of changes in physical climate on forest soilacidification through internal proton production, in determiningsoil solution chemistry. A framework outlining the etiology offorest die-back induced by nitrogen saturation is proposed.
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  • 98
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    Fish physiology and biochemistry 16 (1997), S. 139-155 
    ISSN: 1573-5168
    Keywords: season ; metabolic organization ; muscle ; fish ; temperature ; lipid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study examined how muscle metabolic organization varied during an annual cycle in which rainbow trout (Oncorhynchus mykiss) were held in outdoor holding ponds in which they were exposed to natural changes in temperature (range 0.2 to 15.6°C) and photoperiod. We examined the activities of glycolytic and mitochondrial enzymes in red and white muscle to evaluate whether trout enhance their capacity for lipid and carbohydrate oxidation during cold-acclimization. When assayed at habitat temperature, the enzyme activities generally increased in spring to reach a maximum in summer followed by a decrease in the fall. This led to significantly higher activities at warm than cold periods for all enzymes measured in red muscle and all but one in white muscle. The activities at 10°C provided little evidence for compensatory adjustments of aerobic capacity. Particularly in red muscle, enzyme levels at 10°C were generally lower during cold than warm periods. The variation of enzyme activities throughout the cycle was not due to changes in protein concentration, as the same responses were observed when activities were expressed per g wet mass or per mg protein. Although the aerobic capacity did not increase with cold-acclimatization, the relative capacity for lipid oxidation was higher in winter than in summer trout. In contrast, the relative capacity for aerobic glycolysis was higher in summer than in winter trout. Thus, the metabolic capacities of trout muscle undergo seasonal reorganization.
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  • 99
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    Fish physiology and biochemistry 17 (1997), S. 231-236 
    ISSN: 1573-5168
    Keywords: growth ; salmon ; smoltification ; insulin ; insulin-like growth factor I (IGF-I) ; growth hormone ; thyroxine ; photoperiod ; temperature ; nutrition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 100
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    Plant cell, tissue and organ culture 48 (1997), S. 195-201 
    ISSN: 1573-5044
    Keywords: cytokinins ; in vitro ; Lonicera caerulea ; minerals ; plant tissue culture ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Factors affecting axillary shoot development of blue honeysuckle in vitro were studied with two genotypes, Lonicera caerulea f. caerulea and L. caerulea f. edulis. There was a linear relationship between the concentration of N6-benzyladenine (1.1 to 17.8 μmol l−1) and the number of axillary branches produced (3 to 13 new shoots), biomass production and callus formation of the form caerulea shoot explants. The formation of second-order branches responded most strongly to the cytokinin treatments. Axillary branching was significantly lower at 24/20 °C (mean day maximum/night minimum) than at 26/20 °C. When the cultures were incubated at 28/21 °C, the number of axillary shoots produced was highest, 10.4 and 21.1 in the forms caerulea and edulis, respectively, but apical injuries were more frequent (17 in the form caerulea, 49 in the form edulis) and shoot morphology changed as compared to the shoots developed at lower temperatures. In the form edulis, the production of axillary shoots also increased by diluting the mineral nutrients of the MS medium to 75. Further dilution of minerals to 50 increased the incidence of apical shoot necrosis. It was shown that the stimulation of axillary branching by increased temperature or decreased nutrient supply was not caused by reduced apical dominance related to shoot tip injuries.
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