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  • wheat  (104)
  • taxonomy
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 93 (1999), S. 227-230 
    ISSN: 1570-7458
    Keywords: Rhopalosiphum padi ; cereal aphids ; wheat ; induced responses ; feeding site
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 2
    Electronic Resource
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    Entomologia experimentalis et applicata 92 (1999), S. 157-164 
    ISSN: 1570-7458
    Keywords: plant resistance ; antibiosis ; tolerance ; antixenosis ; Russian wheat aphid ; wheat ; Homoptera ; Aphididae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Russian wheat aphid, Diuraphis noxia (Mordvilko), is one of the most important aphid pests of wheat, Triticum aestivum L., worldwide. Among the various pest management options, plant resistance is an economical management tactic to control D. noxia in cereal crops such as wheat. Researchers have identified D. noxia resistant germplasm and it has been incorporated into wheat. This study compared D. noxia resistance between the ‘Betta’ wheat isolines Betta-Dn1, Betta-Dn2, and Betta-Dn5 and their corresponding donor gene plant introduction (PI) lines PI 137739 (Dn1), PI 262660 (Dn2), and PI 294994 (Dn5). Although the Betta isolines and PI lines showed D. noxia resistance when compared with Betta wheat, the degree of resistance in the isolines to D. noxia was different from their corresponding PI donors. Aphid number, aphid fecundity, and biomass per aphid were not different between Betta-Dn1 and PI 137739 or Betta-Dn2 and PI 262660; however, the same parameters were significantly lower on PI 294994 compared with Betta-Dn5. This indicated that aphid resistance in PI 137739 and PI 262660 was probably governed by a single dominant gene, while the resistance in PI 294994 was controlled by more than one gene. Additionally, plant biomass reduction was aphid density dependent, which suggested that use of appropriate aphid infestation level is important when using plant biomass reduction as an indicator of resistance. Plant resistance categorization showed that there was no detectable difference in antixenosis among the seven lines evaluated. However, the higher aphid fecundity observed on PI 262660 compared with PI 137739 and PI 294994, in addition to no significant differences among the three PIs in plant biomass reduction, suggested PI 262660 was a tolerant line, while PI 137739 and PI 294994 were antibiotic lines. Plant tolerance could not be elucidated among the three Betta isolines using aphid fecundity and plant biomass reduction as indicators.
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  • 3
    ISSN: 1570-7458
    Keywords: sieve element ; salivation ; aphid ; plant resistance ; wheat ; Sitobion fragariae ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extended sieve element salivation (E1 waveform in the electrical penetration graph) is a characteristic activity during early sieve element punctures, particularly in resistant plants. In order to explore a chemically-mediated mechanism of resistance associated with sieve element salivation, we compared the pattern of feeding behaviour of the aphid, Sitobion fragariae (Walker), on two cultivars of the wheat Triticum aestivum L., with different concentrations of hydroxamic acids (Hx). During 24 h of electronic monitoring, aphids dedicated over 50% of the total time to phloem ingestion from the sieve elements. Total time allocated to E1 in the experiment, time to first E1 within the experiment, time allocated to E1 before a sustained phloem ingestion (E2) and the contribution of sieve element salivation to the phloem phase (E1/[E1+E2]) were significantly higher in the high-Hx cultivar. The increased salivation in plants with higher contents of Hx suggests the existence, at least in this system, of a chemically-mediated sieve element constraint.
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  • 4
    ISSN: 1572-9397
    Keywords: evolutionary algorithms ; genetic algorithms ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper shows how evolutionary algorithms can be described in a concise, yet comprehensive and accurate way. A classification scheme is introduced and presented in a tabular form called TEA (Table of Evolutionary Algorithms). It distinguishes between different classes of evolutionary algorithms (e.g., genetic algorithms, ant systems) by enumerating the fundamental ingredients of each of these algorithms. At the end, possible uses of the TEA are illustrated on classical evolutionary algorithms.
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  • 5
    Electronic Resource
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    Springer
    Plant molecular biology reporter 17 (1999), S. 323-331 
    ISSN: 1572-9818
    Keywords: Agrobacterium ; modular vector ; transformation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat (cv Chinese Spring) tissues were transformed using Agrobacterium tumefasciens and a new plasmid modular vector, pMVTBP. We constructed pMVTBP with unique restriction sites connecting (1) the CaMV 35S promoter, (2) a Kozak sequence, (3) the FLAG epitope, (4) the (His)6 epitope, (5) a coding region (for wheat TATA Binding Protein, wTBP) and (6) the CaMV 35S 3′UTR. This vector thus allows easy exchange of different regulatory or coding sequences. Explants of either germinating mature seeds, or immature embryos, were induced to callus for up to two weeks, treated with virulence-induced bacteria for one hour, then regenerated into plantlets. Transient expression of a GUS reporter gene, assayed at about one week, occurred in 10–12% of calluses. Expression of the FLAG-tagged wTBP was also detected, by immunostaining. Stable expression, by selective growth on geneticin, and by GUS expression at about six weeks, occurred in 1–2% of calluses, quite comparable to that achieved by other methods.
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  • 6
    ISSN: 1573-0832
    Keywords: Africa ; Fusarium ; F. moniliforme ; grain ; Lesotho ; mating population ; Nigeria ; taxonomy ; Zimbabwe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Several Fusarium species have been found associated with millet and sorghum in Nigeria, Lesotho and Zimbabwe. Amongst these, some isolates were originally identified as short- and long-chained types of F. nygamai. However, there was some question as to the correct identification of the long chained types. This study reclassified some of the isolates with long microconidial chains as F. moniliforme. Morphologically, these strains do not produce chlamydospores like F. nygamai, but produce swollen hyphal cells or resistant hyphae. The isolates in this study were crossed with the mating-type tester strains of Gibberella fujikuroi (F. moniliforme and G. nygamai (F. nygamai). Of the isolates with long chains of microconidia and other characteristics of F. moniliforme, 36% crossed with mating population ''A'' of G. fujikuroi. Of the isolates with characteristics of F. nygamai, 65% crossed with the testers used to produce the teleomorph of F. nygamai. Mating tests support the separation of the sample population into F. moniliforme and F. nygamai. The results of this study show that genetics can be an aid in resolving some problems in fungal taxonomy.
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  • 7
    Electronic Resource
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    Nutrient cycling in agroecosystems 53 (1999), S. 139-146 
    ISSN: 1573-0867
    Keywords: critical levels of Mn ; soil extractants ; Mn-deficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seven chemical extractants were tested for their relative performance to predict the response of wheat to Mn application in coarse textured alkaline soils of semi-arid region. Five out of the seven extractants were found to be promising for the estimation of critical level of available Mn in these soils, as the amount of Mn extracted by these extractants was positively and significantly correlated with relative grain yield as well as Mn uptake. The critical deficiency level of soil available Mn with 0.005 M DTPA, 0.02% hydroquinone, 0.02 N sodium pyrophosphate, 0.1N H3PO4 and 0.05N HCl+0.025N H2SO4 was 3.1, 13.8, 23.5, 5.3 and 17.8 mg kg-1 soil, respectively. The 1N ammonium acetate and 0.01M CaCl2 were found to be unsuitable extractants for these soils. Further field trials at eight locations with varying levels of Mn deficiency showed successive increase in the grain yield of wheat with foliar Mn application, emphasizing the need for Mn fertilization when wheat is grown on Mn deficient soils.
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  • 8
    Electronic Resource
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    Antonie van Leeuwenhoek 71 (1997), S. 375-378 
    ISSN: 1572-9699
    Keywords: Candida novakii ; taxonomy ; yeasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two strains of an undescribed species of the genus Candida were isolated from decaying wood of Quercus sp. A description of the new species Candida novakii is given.
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  • 9
    ISSN: 1572-9699
    Keywords: phylogeny ; prosthecobacter ; taxonomy ; Verrucomicrobia ; Verrucomicrobiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four strains of nonmotile, prosthecate bacteria were isolated in the 1970s and assigned to the genus Prosthecobacter. These strains were compared genotypically by DNA/DNA reassociation and 16S rDNA based phylogenetic analyses. Genotypic comparisons were complemented with phenotypic characterizations. Together, these studies clearly indicate each Prosthecobacter strain represents a novel species of bacteria. We propose three new species of Prosthecobacter, P. dejongeii strain FC1, P. vanneervenii strain FC2, and P. debontii strain FC3; P. fusiformis is reserved for the type strain of the genus, strain FC4. Additionally, we propose the genera Prosthecobacter and Verrucomicrobium, currently members of the order Verrucomicrobiales, to comprise a novel higher order taxonomic group, the division Verrucomicrobia div. nov. and the class Verrumicrobiae class nov. Many novel members of the Verrucomicrobia, as revealed by molecular ecology studies, await isolation and description.
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  • 10
    ISSN: 1572-9699
    Keywords: keratinophilic fungi ; Neoarachnotheca ; Neoarachnotheca keratinophila ; Nannizziopsis tropicalis ; Onygenales ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Neoarachnotheca is proposed as a new genus of Onygenales. The outstanding generic characteristics are white, spherical ascomata with a wall formed by a network of hyphae and spherical, subhyaline ascospores with an irregular sheath. Nt. keratinophila, the type species, characterized by wavy peridial hyphae has been isolated from marine and river sediments and Myriodontium keratinophilum is its anamorph. Nannizziopsis tropicalis is proposed as a new species based on a strain isolated from soil in Burundi. RFLPs analysis of ITS and 5.8S rDNA support these proposals. The differences with related genera are discussed.
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  • 11
    ISSN: 1572-9699
    Keywords: bioluminescence ; Pseudomonas ; root colonization ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The bioluminescently marked Pseudomonas fluorescens strain 5RL, has been used previously to follow colonisation of soy bean roots (De Weger et al. [1991] Appl. Environ. Microbiol. 57:36-41). In the present paper the method has been further developed and optimized for wheat roots and it is used to get a quick overview of the colonisation patterns of many different root systems at the same time. Colonisation was followed on wheat plants grown in our gnotobiotic sand system (Simons et al., 1996. Mol Plant Microbe Interact 9: 600–607) and the following results were obtained. (i) A spatio-temporal analysis of the colonisation of wheat roots showed that 4 days after planting the highest bacterial activity was observed at the upper part of the root. After 6 days the high bacterial activity at the upper part was further increased, whereas spot-like activities were observed on the lower root parts, possibly due to micro-colonies. (ii) Bacterial mutations causing lack of motility or auxotrophy for amino acids resulted in impaired colonisation of the lower root parts, indicating that motility and prototrophy for the involved amino acid(s) are important factors for wheat root colonisation by strain 5RL. (iii) Coinoculation of strain 5RL with other wild type Pseudomonas strains on the root influenced the colonisation pattern observed for strain 5RL. Colonisation was not visually affected when the competing strain was a poor root coloniser, but was severely reduced when the competing strain was a good root coloniser. The results show that the spatio-temporal colonisation of wheat root by P. fluorescens strain 5RL and derivatives is similar to that of strain WCS365 on tomato. The advantage of the use of lux-marked strains is that the results are obtained much quicker than when conventional methods are used and that the result is supplied as an image of the colonisation pattern of many different roots.
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  • 12
    ISSN: 1572-9761
    Keywords: Coccinellidae ; Aphididae ; wheat ; spatial scale ; species diversity ; numerical response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of prey density, within-field vegetation, and the composition and patchiness of the surrounding landscape on the abundance of insect predators of cereal aphids was studied in wheat fields in eastern South Dakota, USA. Cereal aphids, aphid predators, and within-field vegetation were sampled in 104 fields over a three year period (1988–1990). The composition and patchiness of the landscape surrounding each field were determined from high altitude aerial photographs. Five landscape variables, aggregated at three spatial scales ranging from 2.6 km2 to 581 km2, were measured from aerial photographs. Regression models incorporating within-field and landscape variables accounted for 27–49% of the variance in aphid predator abundance in wheat fields. Aphid predator species richness and species diversity were also related to within-field and landscape variables. Some predators were strongly influenced by variability in the composition and patchiness of the landscape surrounding a field at a particular spatial scale while others responded to variability at all scales. Overall, predator abundance, species richness, and species diversity increased with increasing vegetational diversity in wheat fields and with increasing amounts of non-cultivated lands and increasing patchiness in the surrounding landscape.
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  • 13
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    Integrated pest management reviews 4 (1999), S. 127-143 
    ISSN: 1572-9745
    Keywords: wheat ; stored-grain ; integrated pest management ; aeration ; biological control ; grain sampling ; insect monitoring ; modeling ; area-wide IPM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Management of stored-grain insect pests by farmers or elevator managers should be based upon a knowledge of the grain storage environment and the ecology of insect pests. Grain storage facilities and practices, geographical location, government policies, and marketing demands for grain quality are discussed as factors influencing stored-grain insect pest management decisions in the United States. Typical practices include a small number of grain samples designed to provide grain quality information for segregation, blending and marketing. This low sampling rate results in subjective evaluation and inconsistent penalties for insect-related quality factors. Information on the efficacy of insect pest management practices in the United States, mainly for farm-stored wheat, is discussed, and stored-grain integrated pest management (IPM) is compared to field-crop IPM. The transition from traditional stored-grain insect pest control to IPM will require greater emphasis on sampling to estimate insect densities, the development of sound economic thresholds and decision-making strategies, more selective use of pesticides, and greater use of nonchemical methods such as aeration. New developments in insect monitoring, predictive computer models, grain cooling by aeration, biological control, and fumigation are reviewed, their potential for improving insect pest management is discussed, and future research needs are examined.
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  • 14
    ISSN: 1572-9745
    Keywords: Biological control ; insects ; pathogens ; germination ; taxonomy ; genetic variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pigweeds (Amaranthus spp.) are of economic importance worldwide. In Europe, Amaranthus retroflexus is one of the ten weed species of greatest economic importance. It is a serious problem weed in several field crops (e.g. maize), as well as in vegetables, orchards and grape vines. It is an annual spreading by seeds which have a long viabilityand are dispersed principally by wind and water, but also by machinery. There is great variability in seed germination which renders control with post-emergence herbicides difficult. In addition, triazine herbicide-resistant populations occur in ten European countries. The aim of this subproject of COST action 816 is to investigate the possibilities of classical and inundative biological control of Amaranthus spp., to characterize potentialbiological control agents and to develop methods for their integration with current phytosanitary measures in the target crops. The project was initiated with an extended literaturesurvey followed by field surveys for insects and pathogens associated with Amaranthus spp. in several European countries. Promising isolates of fungal pathogens have been tested ondetached leaves and whole plants, and initial studies on the application of pathogens causing damping off in seedlings have been made. Further, the variability of different provenances ofAmaranthus spp. in response to fungal attack has been investigated
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  • 15
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    Journal of the history of biology 32 (1999), S. 509-555 
    ISSN: 1573-0387
    Keywords: Louis Agassiz ; Spencer Baird ; Charles Girard ; reputation ; taxonomy ; ichthyology ; Museum of Comparative Zoology ; Smithsonian Institution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , History
    Notes: Abstract The reputations of scientists among their contemporaries depend not only on accomplishment, but also on interactions affected by influence and personality. The historical lore of most fields of scientific endeavor preserve these reputations, often through the identification of founders, innovators, and prolific workers whose contributions are considered fundamental to progress in the field. Historians frequently rely on the historical lore of scientists to guide their studies of the development of ideas, exhibiting justifiable caution in reassessing reputations in the light of current knowledge. However, the transmission of historical lore can obscure the relative importance of accomplishment, influence and personality in shaping contemporary reputations, leaving the historian to either accept reputations at face value or attempt to reconstruct the context in which they were created. The science of taxonomy, because of its rules of priority, leaves a relatively accurate record of historical accomplishment through the persistence of taxa in catalogues and faunal guides. These records allow the modern historian an unbiased means to assess the relative accomplishments of historical figures and therefore a means to critically reassess reputations independent of personality and influence. In the historical lore of North American ichthyology, Louis Agassiz at Harvard and Spencer Baird at the Smithsonian emerge as central figures in the early development of the field during the mid-1800s, contributing not only through the quality and quantity of their science, but also through their roles as institutional leaders and mentors to workers who followed. Charles Girard, originally a student of Agassiz's and later a coworker with Baird, receives little notice in the history of ichthyology, and his reputation is that of a minor player in the initial description of the North American fish fauna, and one whose work appears to have been flawed or even careless when compared to his contemporaries. However, a review of both contemporary and modern taxonomic works reveals that Girard's productivity far exceeded that of either Agassiz or Baird. Furthermore, an examination of the tendency of Girard and his contemporaries to introduce synonymous names into the literature, which might reflect careless or uncritical work, suggests that Girard was among the more accomplished workers of hisera, including Agassiz and Baird. Girard's low ranking in the folklore of North American ichthyology, therefore, can not be attributed to discernible shortcomings in his scientific work, but rather to a public and private campaign of criticism waged by Agassiz after Girard's departure from Harvard. While Agassiz's dispute with Girard stemmed from their personal interactions, he expressed them as criticisms of Girard's work, and thus helped shape Girard's scientific reputation as it has been transmitted through the lore of ichthyology. This case study reveals how scientific reputation may not always rest on accomplishment, but can be influenced by personal interactions obscured by time but nonetheless important to history.
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  • 16
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    Journal of chemical ecology 23 (1997), S. 2695-2705 
    ISSN: 1573-1561
    Keywords: Defense ; herbivory ; aphids ; wheat ; Gramineae ; hydroxamic acids ; Defense theory ; Carbon/Nutrient theory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Hydroxamic acids (Hx) are natural products of Gramineae that are associated with cereal resistance to pests. We aimed at characterizing the induction of Hx accumulation in seedlings of wheat,Triticum aestivum, by short-term infestation of the cereal aphid,Rhopalosiphum padi. A load of 25 aphids increased significantly the Hx levels in the infested primary leaf in comparison with control levels. Lower loads did not increase Hx concentration. Aphid infestation lasting 16 hr did not elicit induction of Hx, even after a time-lag of 32 hr to allow the expression of any induced response. Forty-eight hours was the minimum duration of aphid infestation required to trigger Hx induction. The age of the infested tissue (the primary leaf) did not affect induction. Similar increases of Hx were found in unfolding, expanding, and totally expanded primary leaves. It was determined that the regime of nutrient supply (N-intensive nutritive solutions at low and high concentration) to wheat seedlings had no effect on the magnitude of the aphid-induced Hx (N-based secondary metabolites). Results obtained are discussed in the framework of general theories of plant defense allocation.
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  • 17
    ISSN: 1573-5028
    Keywords: ADP-glucose pyrophosphorylase ; anther ; pollen ; male sterility ; water stress ; wheat ; starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In wheat (Triticum aestivum L.), water deficit during meiosis in the microspore mother cells (MMCs) induces pollen abortion, resulting in the failure of fertilization and a reduction in grain set. In stressed plants, meiosis in MMCs proceeds normally but subsequent pollen development is arrested. Unlike normal pollen grains, which accumulate starch during the late maturation phase, stress-affected anthers contain pollen grains with little or no starch. Stress also alters the normal distribution of starch in the anther wall and connective tissue. To determine how starch biosynthesis is regulated within the developing anthers of stressed plants, we studied the expression of ADP-glucose pyrophosphorylase (AGP), which catalyzes the rate limiting step of starch biosynthesis. Two partial-length cDNAs corresponding to the large subunit of AGP were amplified by RT-PCR from anther RNA, and used as probes to monitor AGP expression in developing anthers of normal and water-stressed plants. These clones, WAL1 and WAL2, had identical deduced amino acid sequences and shared 96% sequence identity at the nucleic acid level. In normal anthers, AGP expression was biphasic, indicating that AGP expression is required for starch biosynthesis both during meiosis and later during pollen maturation. AGP expression in stressed anthers was not affected during the first phase of starch accumulation, but was strongly inhibited during the second phase. We conclude from these results that the reduced starch deposition later in the development of stressed pollen could be the result of a lower expression of AGP. However, this inhibition of AGP expression is unlikely to be the primary cause of male sterility because anatomical symptoms of pollen abortion are observed prior to the time when AGP expression is inhibited.
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  • 18
    ISSN: 1573-5028
    Keywords: chloroplast ; in vitro transcription ; light ; psbA ; psbD/C operon ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dynamical aspects of three chloroplast promoters responding to change in light condition were examined in mature chloroplasts of wheat (Triticum aestivum) by in vitro transcription. The wheat psbD/C operon has four distinct promoters, two of which named as D/C-3 and D/C-4 promoters dominantly function in mature chloroplasts to produce the mRNAs encoding D2/CP43 and CP43 alone, respectively. Activity of the D/C-3 promoter in mature chloroplasts was reduced to less than 30% by 24 h dark adaptation and recovered by re-illumination to the original level within 30 to 60 min. The activation of the D/C-3 promoter which requires de novo cytoplasmic protein synthesis was induced by low fluence of light (e.g. 16 µE m-2 s-1), but the extent of activation increased with increasing light fluence. The accumulation of mRNAs from the D/C-3 promoter saturated at 2- to 3-fold higher level within 2 h when the dark-adapted seedlings were transferred to the lig at 72 µE m-2 s-1, concomitant with the increase in rate of D2 synthesis, suggesting that synthesis of D2 in mature chloroplasts is controlled via the D/C-3 promoter activity in a light-dependent way. Activity of the D/C-4 promoter slightly increased in the dark and decreased in the light. Effect of light on the psbA promoter activity was not observed at all in mature chloroplasts. In vitro transcriptional analysis of the D/C-3 promoter with 5′ deletion mutations revealed that at least two cis elements which are located within the sequences of -78 to -47 and -46 to -29 of the transcription initiation site, respectively, act as enhancing elements in the D/C-3 promoter. The light-switching element of the transcription, however, was suggested to be located in the core promoter sequence downstream of the -35 element.
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  • 19
    ISSN: 1573-5028
    Keywords: cold temperature ; dehydration ; greening ; protein kinase ; Triticum aestivum/ ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have identified a new wheat PKABA1/-like protein kinase gene, TaPK3/, that is expressed in greening wheat seedlings. TaPK3 has high sequence homology (97% similarity with some sequence diversity at the 3' end) to the wheat PKABA1 protein kinase mRNA, which is upregulated by cold-temperature treatment, dehydration and abscisic acid (ABA). Use of a TaPK3 gene-specific probe has revealed that TaPK3 is differentially expressed with respect to PKABA1. TaPK3 mRNA accumulates in greening shoot tissue of wheat, but is not affected by dehydration, cold-temperature treatment or ABA. Based on sequence and expression differences, we conclude that expression of the PKABA1/-like protein kinases is not limited to stress responses.
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  • 20
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    Plant molecular biology 34 (1997), S. 643-650 
    ISSN: 1573-5028
    Keywords: callus ; crown tissue ; gene expression ; low temperature ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The low-temperature (2 °C)-specific wheat cDNA, pTACR7, represents a gene designated tacr7 from hard red winter wheat (HRWW; Triticum aestivum L. cv. Winoka). The term low-temperature-specific (LTS) is used because tacr7 is not induced by ABA or stresses such as salt, dehydration, and heat. pTACR7 was isolated by RT-PCR with mRNA from wheat crown tissue, the oligonucleotide primers derived from the barley cognate pHVCR8 (GenBank accession number L28091). Based on the deduced amino acid sequence, TACR7 is highly hydrophobic, with a single transmembrane domain and an amino acid bias for leucine (19%). Thus, the encoded protein TACR7 is unique among low-temperature-regulated wheat proteins described in the literature. Analysis of steady-state levels of tacr7 transcripts (630 nt) showed accumulation in wheat seedlings, crown tissue, and callus cultures after transfer from control (25 °C) to low temperature (2 °C). No detectable transcripts were observed by northern blot hybridization with pTACR7 probe from seedling or callus treated with ABA, salt, dehydration, or heat stress. tacr7 transcripts accumulated during 2 °C exposure to a greater amount in a freeze-resistant HRWW (FR; SDmut 16029) than in a freeze-susceptible HRWW (FS; SDmut 16169) crown tissue, with the largest difference between genotypes being 30% ± 3% at 3 weeks.
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  • 21
    ISSN: 1618-2545
    Keywords: Bruguiera gymnorrhiza ; ecology ; Lanceispora amphibia ; mangrove ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lanceispora amphibia gen. et sp. nov. in the Amphisphaeriaceae is described from senescent and fallen leaves ofBruguiera gymnorrhiza in mangrove forests in the Southwest Islands, Japan. The fungus produces immersed ascomata in leaf tissue, cylindrical asci with an apical ring staining blue with iodine, and oblanceolate ascospores with a septum above the middle. Studies on the fungal succession on the mangrove leaves revealed thatL. amphibia infects senescent leaves on the tree and inhabits intertidal fallen leaves, showing the highest frequency of occurrence at the late stage of decomposition. In culture the optimal conditions for hyphal growth were 20 ppt salinity and 30°C, and those for sexual reproduction were 10 ppt salinity and 25°C. Growth at 0 ppt (fresh water) was depressed. The fungus has amphibious habits, growing on the tree and in intertidal water; and it is adapted to the high osmotic conditions in leaf tissues of the mangrove tree and to the subtropical, brackish water environment of mangrove forests.
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  • 22
    ISSN: 1618-2545
    Keywords: Bauhinus ; Microbotryum ; taxonomy ; Ustilago ; ustomycetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study of the type specimen ofUstilago jehudana resulted in the correction of the diagnosis. The sori are localized in the host gynoecium but not in the anthers. Morphological characters of the sori and ustospores of the later describedU. moenchiae-manticae are identical with these ofU. jehudana. Ustilago moenchiae-manticae is reduced here to a synonym ofU. jehudana. The smut is reported as new to Bulgaria on a new host, viz.,Moenchia erecta. A new combination,Bauhinus jehudanus, is proposed.
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    Mycoscience 40 (1999), S. 73-80 
    ISSN: 1618-2545
    Keywords: Mycena auricoma ; Mycena sect.Radiatae ; Mycena spinosissima ; taxonomy
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    Notes: Abstract Two lignicolous species ofMycena (Agaricales, Basidiomycetes) are described and illustrated from eastern, Japan:Mycena auricoma sp. nov., forming ephemeral coprinoid basidiomata and belonging to sectionRadiatae, was found on a dead fallen twig ofQuercus serrata. It appears to close to a Malaysian species,“Trogia” crinipelliformis. Mycena spinosissima in sectionSacchariferae, new to Japan, was collected from dead bark ofAphananthe aspera, a dead fallen inflorescence ofCryptomeria japonica, and a dead fallen twig ofQuercus serrata.
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  • 24
    ISSN: 1618-2545
    Keywords: cellular slime molds ; dictyostelids ; macrocyst ; mating system ; taxonomy
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    Notes: Abstract Macrocyst formation in the sexual cycle was found in three dictyostelid species:Dictyostelium monochasioides, Polysphondylium candidum, andP. pseudo-candidum. Mating tests suggested thatD. monochasioides andP. pseudocandidum were heterothallic andP. candidum was homothallic. The primary walls of macrocysts had partially or fully degenerated, while the inner walls, believed to be tertiary walls, showed an undulate structure.
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  • 25
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    Mycoscience 38 (1997), S. 55-69 
    ISSN: 1618-2545
    Keywords: discomycetes ; ITS rDNA phylogeny ; morphology ; taxonomy
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    Topics: Biology
    Notes: Abstract The systematics ofScleromitrula andVerpatinia of the family Sclerotiniaceae is reevaluated on the basis of morphological, cultural and molecular criteria.Scleromitrula shiraiana, Verpatinia species andCiborinia candolleana share gross morphological, microanatomical and cultural features which clearly distinguish them from the closely relatedCiborinia andRutstroemia species. Phylogenetic analyses of sequences from the internal transcribed spacer region (ITS1, ITS2, and the 5.8S gene) of nuclear ribosomal DNA demonstrate that the stipitate-capitate specimens ofScleromitrula andVerpatinia species plus the stipitate-cupulateCiborinia candolleana constitute a monophyletic clade separate from a clade including the type species ofCiborinia. Scleromitrula is emended to includeS. shiraiana, the new speciesS. rubicola, C. candolleana, and specimens formerly assigned toVerpatinia. A key to the accepted species ofScleromitrula is provided.
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  • 26
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    Mycoscience 38 (1997), S. 71-73 
    ISSN: 1618-2545
    Keywords: Ceratobasidiaceae ; Japan ; taxonomy ; Ypsilonidium bananisporum
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    Topics: Biology
    Notes: Abstract Ypsilonidium bananisporum sp. nov. belonging to Ceratobasidiales is described and illustrated. This fungus has all the characteristics of the genusYpsilonidium including reticulate-hypochnoid basidiomes, broad hyphae branching at right angles, broadly clavate basidia with two sterigmata, and basidiospores germinating by repetition. It differs from all hitherto known species in the genus by producing suballantoid to banana-shaped basidiospores, measuring 19.5–22×5.5–6 μm.
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  • 27
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    Mycoscience 38 (1997), S. 87-89 
    ISSN: 1618-2545
    Keywords: Israel ; Mycomycetes ; Physarales ; Stemonitales ; taxonomy
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    Topics: Biology
    Notes: Abstract Ten taxa of myxomycetes growing mainly withEucalyptus, oak and pine are described. They were found in Upper Galilee, Mt. Carmel and Central parts of the country and all are new to Israel.
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  • 28
    ISSN: 1618-2545
    Keywords: hyphomycetes ; identification ; taxonomy ; Tuberculariaceae ; Tuberculariales
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    Notes: Abstract Mycoleptodiscus terrestris from black pepper roots in the Dominican Republic is described together with some notes and photomicrographs.
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  • 29
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    Mycoscience 38 (1997), S. 429-431 
    ISSN: 1618-2545
    Keywords: coelomycete ; Japan ; Microsphaeropsis rugospora ; soil fungus ; taxonomy
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    Topics: Biology
    Description / Table of Contents: Abstract In an exploratory survey of soil-borne mitosporic fungi as producers of secondary metabolites useful to the pharmaceutical industry, a pale yellow pycnidial coelomycete was encountered and isolated on potato-dextrose agar. The fungus was characterized as follows: rapid growth on common media, conidiomata which are non-pulvinate, semi-immersed to immersed, nearly globose, glabrous, with a slightly papillate ostiole; pale yellowish brown, translucent, membranaceous peridium; discrete, ampulliform, monophialidic conidiogenous cells; and onecelled, dark brown, globose, thick-walled, rugose conidia which germinate very easily. In accordance with this profile, our isolate is included in the genusMicrosphaeropsis Höhnel. (Morgan-Jones, 1974a, b; Sutton, 1977, 1980; Morgan-Jones and White, 1987; Heiny et al., 1992; Katumoto, 1992). However, it proved to be sufficiently different from all described species ofMicrosphaeropsis to warrant its description as a new species.
    Notes: Abstract A new species ofMicrosphaeropsis (Sphaeropsidales, Coelomycetes),M. rugospora, is described and illustrated. This fungus is characterized by its rapid growth on common media such as oatmeal and potato-carrot agars; semi-immersed to immersed, nearly globose, papillate pycnidias; pale yellowish brown, translucent, membranaceous peridium; monophialidic, ampulliform conidiogenous cells; and one-celled, dark brown, globose conidia ornamented with distinct tubercles. The holotype was isolated from the cultivated soil in Tanegashima Island, southern Japan.
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    Mycoscience 40 (1999), S. 189-191 
    ISSN: 1618-2545
    Keywords: bamboo fungus ; hyperparasitism ; hyphomycetes ; mycoparasites ; taxonomy
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    Topics: Biology
    Notes: Abstract Cryptophiale sphaerospora sp. nov. is described and illustrated based on a single collection attached to a synnematous fungus,Janetia synnematosa, from a dead bamboo culm. It differs from other species ofCryptophiala in having spherical to subspherical conidia and a cerebroid layer of phialides. The overall morohology of this species is smaller than that of previously described species.
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  • 31
    ISSN: 1615-6110
    Keywords: Rosaceae ; Rosa sect.Caninae ; Systematics ; taxonomy ; genetic variation ; hemisexual ; apomixis
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    Notes: Abstract Difficulties in delimiting well-defined entities in the dogroses (Rosa sect.Caninae) has resulted in very variable taxonomic treatments. The present study was undertaken to provide a background for taxonomy as well as plant breeding. Morphometric diversity was analysed on seedlings obtained from field collections in South Sweden of three species,Rosa dumalis, R. rubiginosa andR. villosa. A canonical variates analysis showed that the three species are relatively distinct whereas two subspecies ofR. dumalis were less well discriminated. Analyses of variance demonstrated that intraspecific variation is pronounced inR. dumalis and, to a lesser extent, inR. villosa.
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  • 32
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    Plant systematics and evolution 205 (1997), S. 241-264 
    ISSN: 1615-6110
    Keywords: Boraginaceae ; Boragineae ; Anchusella ; A. variegata ; A. cretica ; Lycopsis ; Anchusa ; Mediterranean flora ; macromorphology ; micromorphology ; karyology ; taxonomy
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    Notes: Abstract The two closely related speciesLycopsis variegata andAnchusa cretica, formerly placed inAnchusa subg.Rivinia, were compared with the type species ofLycopsis andAnchusa, on the basis of a set of macro and microcharacters. The presence of only two fertile stamens as well as other peculiar characters in flower structure, androecium, gynoecium, pollen and fruit, supports the institution of the new genusAnchusella, consisting ofA. variegata andA. cretica. Karyological and eco-chorological aspects are consistent with morphological data in pointing to the autonomy of this genus, which appears characterized by autapomorphic, advanced traits.
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    Plant systematics and evolution 208 (1997), S. 1-9 
    ISSN: 1615-6110
    Keywords: Fabaceae ; Vicia faba ; V. kalakhensis ; Seed albumins ; HPLC ; taxonomy
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    Notes: Abstract Previously reported electrophoretic seed albumin data have shown an unexpected association ofVicia faba withV. kalakhensis. In the present work, seed albumins ofV. faba (subsp.paucijuga and subsp.faba) were compared with those ofV. kalakhensis using ionexchange (IE) and reversed-phase (RP) high-performance liquid chromatography (HPLC). Two subspecies ofV. faba displayed similar seed albumin profiles. On the other hand, seed albumin profiles ofV. faba andV. kalakhensis showed no major protein peak in common either in IE-HPLC or RP-HPLC chromatograms. The reported differences in seed albumin composition ofV. faba andV. kalakhensis are consistent with other taxonomical data showingV. faba to be genetically distant from the wild relatives.
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    Plant systematics and evolution 208 (1997), S. 239-248 
    ISSN: 1615-6110
    Keywords: Fabaceae ; Vicia ; sect.Hypechusa ; sect.Peregrinae ; Electrophoresis ; seed albumins ; taxonomy
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    Notes: Abstract This work is a continuation of electrophoretic investigations aimed at revealing a wild relative ofVicia faba. Electrophoretic analysis (PAGE) of seed albumins covered 52 accessions representing eightVicia species of sect.Hypechusa and two species of sect.Peregrinae. Most of the examined species showed an intraspecific variation due to differences between accessions and/or individual variation within accessions. In spite of the intraspecific variation, marked interspecific differences were recorded. However, none of the investigated species displayed electrophoretic seed albumin patterns similar to those reported earlier forV. faba. Contribution of the obtained results to characterization of the examined taxa is discussed.
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  • 35
    ISSN: 1615-6110
    Keywords: Bryophyta ; Mitochondria ; nad5 gene ; group I intron ; molecular phylogeny ; taxonomy
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    Notes: Abstract In contrast to animals, the slowly evolving mitochondrial nucleotide sequences of plants appear well suited to investigate phylogenetic relations between old taxonomic groups. Analysis ofnad5 gene sequences in 47 bryophytes, the living representatives of very early land plants, confirm this assessment. Statistically reliable phylogenetic trees are obtained with different mathematical approaches. A group I intron sequence conserved in thenad5 gene of all 30 mosses and 15 liverworts investigated supports a sister group relationship of the two classes. The intron sequence adds phylogenetic information for fine resolution on top of the conserved exon sequences down to the level of classically defined orders or families, respectively. This intron is not present in the hornwortsAnthoceros husnotii andA. punctatus. The results allow statements on diverging taxonomic interpretations and support the monophyly of the liverworts, mosses, Jungermanniidae, Marchantiidae and Bryidae, and allow recognition of subclasses like Hypnanae and Dicrananae. Among the mosses, the derived orders (subclass Bryidae) are confidently set apart from the Sphagnales, Andreaeales, Polytrichales and Tetraphidales with Buxbaumiales occupying a mediating position. Among the liverworts, full support is found for the classic separation of simple (jungermanniid) and complex thalloid (marchantiid) species with a strikingly low mitochondrial sequence divergence among the latter.
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  • 36
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    Journal of applied phycology 11 (1999), S. 411-419 
    ISSN: 1573-5176
    Keywords: Bangiales ; Porphyra life history ; culture ; conchocelis ; conchosporangia ; spores ; neutral conchospores morphology ; taxonomy
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    Notes: Abstract Since 1992, as part of a study to circumscribe the genus Porphyra in New Zealand, an extensive culture programme has been developed at the Museum of New Zealand Te Papa Tongarewa. This collection currently houses more than 800 cultures and 150strains. We currently recognise at least 20 species in New Zealand, most of which are undescribed. Close observation of reproduction and the development of conchocelis-phase filaments grown in the laboratory, under controlled conditions of temperature, irradiance and photoperiod, has enabled the recognition of characters that can assist in species separation. The comparative taxonomic value of a range of such characters is discussed, including reporting a novel reproductive mode.
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  • 37
    ISSN: 1573-5176
    Keywords: distribution ; harvesting ; Porphyra ; recruitment ; seasonality ; South Africa ; taxonomy
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    Notes: Abstract Although Porphyra is commercially farmed in many countries, in South Africa only small harvests of wild populations for sale as nori have been carried out. The discovery that Porphyra improves growth of South African abalone (Haliotis midae) farmed inland-based tanks has led to increased pressure to harvest wild populations. This paper reports on a survey of the distribution and seasonality of Porphyra in the southern Western Cape. Porphyrawas present at all sites surveyed, and showed considerable temporal variation. A significant amount of the Porphyra present is in reserves and therefore protected from harvesting. Close rexamination of one site revealed seasonal populations of Porphyra that occupied different niches dependent on season. Recruitment peaked in spring and autumn, leading to dense summer and winter populations. Summer populations generally grew lower in the eulittoral than winter populations. No pattern in the mortality of larger thalli wasde tected, though sporeling mortality was high following recruitment peaks. Although it seems that most sites in the southern Western Cape are suitable for harvesting, the taxonomy of the genus in the region urgently needs revision if populations are to be appropriately managed.
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  • 38
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    Mycoscience 40 (1999), S. 165-183 
    ISSN: 1618-2545
    Keywords: freshwater fungi ; Halosphaeriaceae ; taxonomy ; tropics
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    Topics: Biology
    Notes: Abstract The generic concepts inAniptodera, Halosarpheia, Nais andPhaeonectriella are discussed and those species occurring in freshwater are described, some with illustrations. Five new species:Aniptodera lignicola, A. mauritaniensis, A. megalospora, A. palmicola, Phaeonectriella appendiculata are also described. A key to the freshwater species in the above genera and a synoptic diagram of their ascospores are provided.
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  • 39
    ISSN: 1618-2545
    Keywords: Ascomycota ; marine fungi ; new species ; taxonomy
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    Notes: Abstract A new speciesDactylospora mangrovei is described and illustrated and compared withD. haliotrepha andD. canariensis, all marine inhabiting species.Dactylospora haliotrepha andD. mangrovei are tropical/sub-tropical mangrove species collected in Hong Kong, Malaysia, Taiwan and Thailand, whileD. canariensis was described from temperate waters. A map of the geographical distribution of the three marineDactylospora species is presented.
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  • 40
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    Mycoscience 40 (1999), S. 185-188 
    ISSN: 1618-2545
    Keywords: Amphisphaeria ; ascomycetes ; bamboo fungi ; taxonomy
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    Notes: Abstract A new species ofArecophila and a species previously known asAmphisphaeria coronata are described and illustrated from dead culms of bamboo.Arecophila bambusae sp. nov. is distinct in the genus in having ellipsoidal ascospores with slightly round ends, and asci with a narrow subapical ring.Arecophila coronata comb. nov. has asci with a wedgeshaped apical ring and weakly striated ascospores enclosed in wide mucilaginous sheath.
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    Molecular breeding 5 (1999), S. 561-568 
    ISSN: 1572-9788
    Keywords: wheat ; milling yield ; QTL mapping ; RFLP ; microsatellite
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A partial genetic linkage map constructed using 150 single seed descent (SSD) lines generated from a cross between the hexaploid wheat varieties ‘Schomburgk’ and ‘Yarralinka’ was used to identify loci controlling milling yield. Milling yield data were obtained using seed collected from field trials conducted at different sites over two seasons. The estimated broad-sense heritability of milling yield in this population was calculated as 0.48. In the preliminary analysis, two regions were identified on chromosomes 3A and 7D, which were significantly associated with milling yield and accounted for 22% and 19% of the genetic variation, respectively. Bulked segregant analysis in combination with AFLP identified other markers linked to these loci, as well as an additional region on chromosome 5A, which accounted for 19% of the genetic variation. The applicability of these markers as selection tools for breeding purposes is discussed.
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  • 42
    ISSN: 1572-9788
    Keywords: insect resistance ; aphids ; GNA ; lectins ; transgenic plants ; wheat
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Transgenic wheat plants containing the gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under the control of constitutive and phloem-specific promoters were generated through the particle bombardment method. Thirty-two independently derived plants were subjected to molecular and biochemical analyses. Transgene integration varied from one to twelve estimated copies per haploid genome, and levels of GNA expression from 0 to ca. 0.2% of total soluble protein were observed in different transgenic plants. Seven transgenic plants were selected for further study. Progeny plants from these parental transformants were selected for transgene expression, and tested for enhanced resistance to the grain aphid (Sitobion avenae) by exposing the plants to nymphal insects under glasshouse conditions. Bioassay results show that transgenic wheat plants from lines expressing GNA at levels greater than ca. 0.04% of total soluble protein decrease the fecundity, but not the survival, of grain aphids. We propose that transgenic approaches using insecticidal genes such as gna in combination with integrated pest management present promising opportunities for the control of damaging wheat pests.
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  • 43
    ISSN: 1572-9788
    Keywords: wheat ; DNA markers ; yellow rust resistance ; Yr17
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The Yr17 gene, which is present in many European wheat cultivars, displays yellow rust resistance at the seedling stage. The gene introduced into chromosome 2A from Aegilops ventricosa was previously found to be closely linked (0.5 cM) to leaf and stem rust resistance genes Lr37 and Sr38, respectively. The objective of this study was to identify molecular markers linked to the Yr17 gene. We screened with RAPD primers, for polymorphism, the DNAs of cv. Thatcher and the leaf rust-resistant near-isogenic line (NIL) RL 6081 of cv. Thatcher carrying the Lr37 gene. Using a F2 progeny of the cross between VPM1 (resistant) and Thésée (susceptible), the RAPD marker OP-Y15580 was found to be closely linked to the Yr17 gene. We converted the OP- Y15580 RAPD marker into a sequence characterized amplified region (SCAR). This SCAR marker (SC-Y15) was linked at 0.8 ± 0.7 cM to the Yr17 resistance gene. We tested the SC-Y15 marker over a survey of 37 wheat cultivars in order to verify its consistency in different genetic backgrounds and to explain the resistance of some cultivars against yellow rust. Moreover, we showed that the Xpsr150-2Mv locus marker of Lr gene described by Bonhomme et al. [6] which possesses A. ventricosa introgression on the 2A chromosome was also closely linked to the Yr17 gene. Both the SCAR SC-Y15 and Xpsr150-2Mv markers should be used in breeding programmes in order to detect the cluster of the three genes Yr17, Lr37 and Sr38 in cross progenies.
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  • 44
    ISSN: 1572-9788
    Keywords: leaf rust ; molecular marker ; receptor-like kinase ; resistance breeding ; resistance gene ; wheat
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We recently showed that the Lr10 wheat leaf rust resistance gene cosegregated with the candidate resistance gene Lrk10 which encodes a putative receptor-like kinase. The aim of this study was to develop Lrk10-derived molecular markers for the detection of the Lr10 gene in breeding material. Different subfragments of Lrk10 were tested as RFLP markers for the Lr10 resistance gene. The most specific fragment (Lrk10-6) was converted into the PCR-based STS marker STSLrk10-6. Both the RFLP and the STS marker did not give a signal with near isogenic lines containing a different Lr gene. The applicability of these markers for the detection of Lr10 in genetically diverse material was tested with 62 wheat and spelt breeding lines, mostly from European breeding programmes. Twelve varieties known to have Lr10 showed the same alleles as the originally characterized line ThatcherLr10. Most of the lines with unknown composition at the Lr10 locus had a null allele with both the RFLP marker Lrk10-6 and the marker STSLrk10-6 whereas 20% of the lines had a different allele. For six lines, including a traditional spelt variety derived from a landrace, both markers showed the same allele as Thatcher Lr10. Artificial infections of these lines with an isolate avirulent on Lr10 resulted in a hypersensitive reaction of all these lines, indicating also the presence of the Lr10 resistance gene. These data demonstrate that the markers derived from sequences of Lrk10 are highly specific for the Lr10 gene in breeding material of very diverse genetic origin. The markers will allow the defined deployment of Lr10 in wheat breeding programmes and will contribute to the elucidation of the role of Lr10 in polygenic resistances against leaf rust.
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    Molecular breeding 3 (1997), S. 177-181 
    ISSN: 1572-9788
    Keywords: bulked segregant analysis ; H21 ; near-isogenic lines (NILs) ; RAPD ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Near-isogenic lines in conjunction with bulked segregant analysis were used to identify a DNA marker in wheat (Triticum aestivum L.) associated with the H21 gene conferring resistance to biotype L of Hessian fly [Mayetiola destructor (Say)] larvae. Near-isogenic lines were developed by backcross introgression BC3F3:4 (‘Coker 797’ * 4 / ‘Hamlet’) and differed by the presence or absence of H21 (on 2RL) derived from ‘Chaupon’ rye (Secale cereale L.). Bulked DNA samples were prepared from near-isogenic lines and BC3F2 population individuals segregating for reaction to Hessian fly biotype L and screened for random amplified polymorphic DNA markers using 46 10mer primers. Random-amplified polymorphic DNA markers from resistant and susceptible individuals and parental lines were scored and these data were used to identify a 3 kb DNA fragment that was related to the occurrence of H21. This fragment was amplified from DNA isolated from Hamlet, a near-isogenic line carrying 2RL, and bulked DNA from resistant BC3F2 individuals, but not from the recurrent parent Coker 797 or DNA bulks from susceptible BC3F2 plants. Analysis of 111 BC3F2 segregating individuals and BC3F2:3 segregants confirmed the co-segregation of the 3 kb DNA marker with the H21 resistance gene to Hessian fly. Use of this marker could facilitate more rapid screening of plant populations for Hessian fly resistance and monitoring the introgression of H21.
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    European journal of plant pathology 105 (1999), S. 629-641 
    ISSN: 1573-8469
    Keywords: wheat ; Triticum spp. ; Septoria tritici ; septoria tritici blotch of wheat Stagonospora nodorum ; stagonospora nodorum blotch of wheat
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 47
    ISSN: 1573-5117
    Keywords: taxonomy ; Ostracoda ; China ; ancient lakes ; saline lakes ; biogeography ; checklist ; Fabaeformiscandona danielopoli n.sp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fabaeformiscandona danielopoli n.sp. isdescribed from the Chinese Lake Erhai, a freshwaterlagoon of the ancient saline lake Qinghai. The speciesbelongs to the acuminata-group of the genus andis characterized by the shape of both male and femalevalves and by the morphology of the female genitallobe and of lobe ‘a’ in the hemipenis. The new speciesis quite rare in its type locality and might beendemic to the ancient Qinghai basin. A checklist ofRecent non-marine Chinese ostracods is offered inappendix.
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    Hydrobiologia 365 (1997), S. 33-46 
    ISSN: 1573-5117
    Keywords: Phylogeny ; cladistics ; taxonomy ; systematics ; classification ; evolution ; history ; chronicle ; Nemertea ; Hoplonemertea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract I review how some influential nemertean systematistshave perceived and illustrated phylogenetic trees andargue that the nineteenth century nemerteantaxonomists still influence many contemporarynemertean taxonomists to a high degree. By showing hownineteenth century systematics differs from moremodern views on trees, I hope to convey the advantagesof a cladistic approach to tree-thinking and nemerteansystematics. Furthermore I propose a systematizationof the Eureptantia that illustrates the cladisticapproach to tree-thinking but, more importantly, isalso a better representation of eureptantic phylogenythan previous classifications.
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    Hydrobiologia 356 (1997), S. 157-163 
    ISSN: 1573-5117
    Keywords: Lecanidae ; N.E. India ; Tripura ; taxonomy ; distribution
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    Topics: Biology
    Notes: Abstract Thirty-five species of the family Lecanidae are examined from Tripura state in North-Eastern India. Of these, Lecane levistyla and L. scutata are interesting cold-water forms; L. batillifer is an Australasian element; L. acanthinula and L. sinuata are Oriental endemics and L. braumi, L. lateralis and L. simonneae are palaeotropical species. The lecanid fauna also includes the pantropical L. thienemanni and L. sola while the remainder are cosmopolitan and tropicopolitan elements.
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  • 50
    ISSN: 1573-5117
    Keywords: Branchiopoda ; Branchinella ; taxonomy ; morphology ; India
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fairy shrimp Branchinella kugenumaensis (Ishikawa) was long considered a widely, though disjunctly, distributed species occurring from Japan through eastern Asia to southern India. Attempts by Raj (1951, 1961) to make the Indian populations a new variety (B. k. var. madurai) on the basis of antennal and frontal appendage morphology and on its distribution pattern, were considered unconvincing by later authors. Our new comparison of Japanese and Indian specimens has revealed several differences. The resting egg shells of B. kugenumaensis from Japan have irregular polygonal fields; whereas, the shells of the Indian taxon have lip-like units covered with spinules. Furthermore, there are lobes lateral to the basal penes in the Indian specimens that are lacking in the Japanese taxon. As well as differences in antennal and frontal appendage morphology. These differences correspond with previous illustrations of populations from both geographic regions and lead us to consider Raj's proposed subspecies as a valid species. This brings the number of accepted species in the genus Branchinella to 34. More specimens will need to be evaluated from the area between India and Japan to determine if B. maduraiensis is endemic to the Indian subcontinent or is more broadly distributed on the Asian mainland.
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    Hydrobiologia 360 (1997), S. 47-54 
    ISSN: 1573-5117
    Keywords: taxonomy ; ecology ; caves ; Chydoridae ; Slovenia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Alona stochi n.sp., a third blind cave-dwellingcladoceran from the Dinaric region, was found onseveral occasions in a small cave 30 km south-east ofLjubljana (Slovenia). It is related to Alonahercegovinae Brancelj, 1990 and A. sketiBrancelj, 1992. Several characters suggest that itbelongs to a primitive group within the genus Alona.A. stochi was found in a 'semi-cave' environment, accompanied by a cave-associated fauna(Amphibia: Proteus anguinus, Decapoda: cf. Troglocaris schmidti, Copepoda: Troglodiaptomussketi, Diacyclops tantalus, charon,Elaphoidella stammeri).
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    Hydrobiologia 360 (1997), S. 55-61 
    ISSN: 1573-5117
    Keywords: Crustacea ; Anomopoda ; Moinidae ; Moina ephemeralis ; taxonomy ; cladocerans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Moina ephemeralis n.sp. is described from SouthSlovakia. It belongs to the Moinagouldeni-lipini group. Together with Moinamacrocopa (Straus, 1820) it is the second specieswith a 2-egged ephippium from Central Europe. Thespecies was recorded in the plankton of a highlyeutrophic fish pond.
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  • 53
    ISSN: 1573-5117
    Keywords: Cladocera ; chydoridae ; taxonomy ; Mexico ; aloninae
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    Topics: Biology
    Notes: Abstract Spinalona anophtalma n. gen. n. sp. is describedon parthenogenetic and ephippial females andmales from material collected in a temporary lagoonlocated in the Neovolcanic Province from Mexico at analtitude of 2507 m above sea level. It ischaracterized by a strong armature of the antenna,postabdomen and postabdominal claw, no compound eyeor ocellus, the exopod of thoracic limb IIIwith only four setae and that of P5 with only three setae.This new taxon has no relationwith blind Alona from hypogean habitats.
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  • 54
    ISSN: 1573-5117
    Keywords: marine nematodes ; Draconematidae ; Bathychaetosoma ; taxonomy ; revision ; phylogeny
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    Topics: Biology
    Notes: Abstract The family Draconematidae is reviewed. Diagnoses of all taxa are updated or emended, basedupon an evaluation of diagnostic features. Aphylogenetic analysis at the genus level based onparsimony suggested that Tenuidraconema belongsto the Draconematinae. A new genus Bathychaetosoma is erected to accomodate B.uchidai (Kito, 1983). It is characterized by acephalic region with a smooth, non-thickened cuticleand numerous cephalic adhesion tubes located posteriorto the head region and extending over more than twohead diameters along the cervical region.
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  • 55
    ISSN: 1573-5117
    Keywords: Diaptomidae ; Copepoda ; Calanoida ; taxonomy ; Allodiaptomus rarus n.sp. ; Thailand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A brief taxonomic review is made of the diaptomid copepods of Thailand. The updated list contains 21 species; the records of some species, however, are unreliable. Neodiaptomus botulifer Kiefer, N. yangtsekiangensis Mashiko, and Allodiaptomus calcarus Shen & Tai are redescribed. The extensive morphologic variability observed in N. botulifer casts serious doubt on the validity of Neodiaptomus malaindosinensis Lai & Fernando; hence the synonymy of these two species is discussed. It is clarified that Lai & Fernando (1981) and others had erroneously identified and/or described N. yangtsekiangensis and A. calcarus as Arctodiaptomus bacillifer (Koelbel) and Neodiaptomus mephistopheles Brehm, respectively, and that the occurrence of the latter two species in Thailand is hardly likely. Mongolodiaptomus uenoi (Kikuchi) and Heliodiaptomus elegan Kiefer are reported for the first time from Thailand. Also included in this paper is an illustrated description of a new species, Allodiaptomus rarus n.sp., which is closely related to A. calcarus Shen & Tai.
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    Hydrobiologia 365 (1997), S. 149-156 
    ISSN: 1573-5117
    Keywords: Nemertea ; North America ; taxonomy ; Micrura leidyi ; Fragilonemertes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An elongate, pencil-shaped, burrowing heteronemerteanthat fragments readily is commonly encountered in clamflats and sometimes in sandy beaches of the NewEngland states. It is usually a reddish color, bearsa caudal cirrus, and has been routinely recorded asMicrura leidyi. Histological examination ofdeep red to reddish purple individuals proved them tobe intertidal specimens of the normally deep waterTarrhomyos luridus. Living pink to redindividuals could be divided into two groups on thebasis of color pattern, but were indistinguishablehistologically. Comparison with Micrurafasciolata, the type species of the genus,showed morphological differences that do not representinterspecific variation, but indicate a separategeneric placement.
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    Plant molecular biology 35 (1997), S. 167-177 
    ISSN: 1573-5028
    Keywords: comparative genetics ; rice ; barley ; wheat ; Arabidopsis ; flowering time (heading date) ; photoperiod ; vernalization ; earliness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of genes controlling flowering time (heading date) contributes to our understanding of fundamental principles of plant development and is of practical importance because of the effects of flowering time on plant adaptation and crop yield. This review discusses the extent to which plants may share common genetic mechanisms for the control of flowering time and the implications of such conservation for gene isolation from the major cereal crops. Gene isolation may exploit the small genome of rice in map-based approaches, utilizing the conservation of gene order that is revealed when common DNA markers are mapped in different species. Alternatively, mechanisms may be conserved within plants as a whole, in which case genes cloned from the model dicot Arabidopsis thaliana provide an alternative route.
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    Journal of systems integration 9 (1999), S. 167-185 
    ISSN: 1573-8787
    Keywords: flexibility ; complexity ; systems approach ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract In this paper we present a taxonomy of manufacturing problems, labeled in a general sense as Design, Production, or Distribution problems. One or more basic systems concepts, such as complexity and adaptation, attach themselves to each such problems. By combining the hierarchical Design—Production—Distribution idea with system concepts, we establish the fact that there is, indeed, a significant systems component to most problems of modern manufacturing.
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    Plant systematics and evolution 204 (1997), S. 225-232 
    ISSN: 1615-6110
    Keywords: Saxifragaceae s. l. ; Eremosyne pectinata ; rbcL sequence analysis ; chloroplast DNA ; molecular systematics ; phylogenetic analysis ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Abstract Earlier analyses ofrbcL sequences clarified phylogenetic relationships of 16 of 17 subfamilies ofSaxifragaceae s. l. This study investigates the affinities of the monotypicEremosynoideae, the only subfamily ofSaxifragaceae s. l. not examined previously forrbcL sequence variation. Our analyses suggest thatEremosyne is only distantly related to core members ofSaxifragaceae s. l. (i.e.Saxifragoideae = Saxifragaceae s. str.); it is allied instead with members ofAsteridae. A particularly close relationship is indicated betweenEremosyne andEscallonia, another traditional member ofSaxifragaceae s. l. that also appears as part ofAsteridae. Inclusion ofEremosyne withinAsteridae is in agreement with embryology, as well as with 18S rDNA sequence data.
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    Plant systematics and evolution 214 (1999), S. 91-102 
    ISSN: 1615-6110
    Keywords: Asteraceae ; Lactuceae ; Aetheorhiza ; Launaea ; Reichardia ; Sonchus ; Pollen ; taxonomy ; harmomegathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pollen morphology of the taxa belonging to the generaAetheorhiza Cass.,Launaea Cass.,Reichardia Roth andSonchus L. in the Iberian Peninsula has been studied with light and electron microscopy. The pollen is 3(-4)-zonocolporate and echinolophate (without polar lacunae, but in general with prelacunae), with equatorial ridges and 15–20 lacunae: 3–4 poral, 6–8 abporal and 6–8 paraporal. Small to medium size, P × E = 19−36 × 23–42 µm; sometimes two different sizes have been found. Exine 3–9 µm thick and ornamentation microreticulate and echinate. The results clearly show the relationships between genera.
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  • 61
    ISSN: 1615-6110
    Keywords: Verbenaceae ; Lamiaceae ; Iridoid glucosides ; cladistic analysis ; taxonomy
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    Topics: Biology
    Notes: Abstract The familiar limits ofVerbenaceae are controversial, ranging from a sensu lato arrangement of seven subfamilies until a sharply defined sensu stricto group consisting of only some genera from subfam.Verbenoideae. Iridoid contents of genera belonging toVerbenaceae s. l. were examined through a cladistic analysis, using outgroup analysis to evaluate character polarity within the ingroup. The cladogram obtained shows the distribution of the species according to their iridoid content.
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    Plant systematics and evolution 214 (1999), S. 161-186 
    ISSN: 1615-6110
    Keywords: Rubiaceae ; Rubioideae ; Angiosperms ; cladistics ; DNA sequences ; phylogeny ; rps16 intron ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The phylogeny of the subfamilyRubioideae (Rubiaceae) was estimated from sequence variation in therps16 intron (cpDNA) in 143 ingroup and 5 outgroup taxa. The analysis largely confirms a recent one based onrbcL sequences, but branch support is often much stronger. Three of the traditional subfamilies are supported,Rubioideae, Cinchonoideae s. str., andIxoroideae s. l. while there is no support forAntirheoideae. TheRubioideae are the sister group of all otherRubiaceae and comprise the tribesAnthospermeae, Coccocypseleae, Cruckshanksieae, Coussareeae, Gaertnereae, Hedyotideae, Knoxieae, Morindeae, Ophiorrhizeae, Paederieae, Pauridiantheae, Perameae, Psychotrieae, Rubieae, Spermacoceae, Theligoneae, andUrophylleae. TheHamelieae andHillieae belong to theCinchonoideae. Rachicallis andSiemensia should be transferred from theHedyotideae to theCinchonoideae. ThePauridiantheae, Urophylleae, Ophiorrhizeae, andRaritebe form the basalmost subclade of theRubioideae. The second basalmost clade consists of the generaLasianthus andPerama. The third basalmost clade consists of the tribesCoussareeae, Coccocypseleae andCruckshanksieae, and the generaDeclieuxia andHindsia. The tribesKnoxieae, Anthospermeae, Argostemmateae, Paederieae, Theligoneae, Rubieae, Hedyotideae, andSpermacoceae are members of one clade. TheKnoxieae are monophyletic ifOtiophora, Otomeria, andPentas are included. The tribeAnthospermeae is supported as monophyletic, but its subtribes are not. ThePaederieae, together withTheligonum, form a paraphyletic grade basal to theRubieae. TheHedyotideae, includingSchismatoclada, form a grade at the base of theSpermacoceae. TheGaertnereae are monophyletic and distinct from thePsychotrieae. TheMorindeae are monophyletic and includeDamnacanthus andMitchella. Schradera is the sister group of theMorindeae. ThePsychotrieae are monophyletic when theGaertnereae, Lasianthus, andDeclieuxia are excluded. The recognition of a subtribeHydnophytineae leaves the rest of thePsychotrieae paraphyletic.Psychotria is paraphyletic with respect to all other genera of the tribe. Approximately 50 genera are here classified for the first time based on molecular data.
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    Plant systematics and evolution 219 (1999), S. 199-208 
    ISSN: 1615-6110
    Keywords: Brexioideae ; Brexia ; Ixerba ; Roussea ; Saxifragaceae s. l. ; 18S rDNA andrbcL sequence analysis ; molecular systematics ; phylogenetic analysis ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previous molecular phylogenetic analyses have demonstrated that Saxifragaceae sensu lato are polyphyletic, with component lineages scattered throughout the eudicots. As part of our effort to elucidate the relationships of members of Engler and Prantl's Saxifragaceae s. l., we undertook a molecular systematic study of subfamily Brexioideae, which comprises three genera:Brexia, Ixerba, andRoussea. Not all taxonomic treatments have concurred, however, in placing these genera together. To elucidate relationships among these three genera as well as their relationships to other angiosperms we constructed large data sets ofrbcL, 18S rDNA, andrbcL + 18S rDNA sequences. Our phylogenetic analyses indicate clearly that Brexioideae are polyphyletic.Brexia is part of a celastroid clade that also includesParnassia, Lepuropetalon, and Celastraceae.Ixerba appears as sister to a large eurosid I clade;Roussea appears as part of Asterales. Molecular data, therefore, indicate that Brexioideae are a polyphyletic assemblage and component genera should ultimately be incorporated into other groups. Our studies continue to demonstrate the polyphyly not only Saxifragaceae s. l., but also of its constituent subfamilies.
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    Plant molecular biology 39 (1999), S. 915-926 
    ISSN: 1573-5028
    Keywords: programmed cell death ; wheat ; endosperm ; ethylene ; nucleases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Although maize endosperm undergoes programmed cell death during its development, it is not known whether this developmental feature is common to cereals or whether it arose inadvertently from the selection process that resulted in the enlarged endosperm of modern maize. Examination of wheat endosperm during its development revealed that this tissue undergoes a programmed cell death that shares features with the maize program but differs in some aspects of its execution. Cell death initiated and progressed stochastically in wheat endosperm in contrast to maize where cell death initiates within the upper central endosperm and expands outward. After a peak of ethylene production during early development, wheat endosperm DNA underwent internucleosomal fragmentation that was detectable from mid to late development. The developmental onset and progression of DNA degradation was regulated by the level of ethylene production and perception. These observations suggest that programmed cell death of the endosperm and regulation of this program by ethylene is not unique to maize but that differences in the execution of the program appear to exist among cereals.
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    Plant and soil 188 (1997), S. 309-317 
    ISSN: 1573-5036
    Keywords: chlorsulfuron ; nutrient solution ; roots ; uptake ; wheat ; zinc deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat plants differing in zinc efficiency (Excalibur; Zn-efficient, Gatcher and Durati; Zn-inefficient) were grown in HEDTA chelate-buffered nutrient solution in controlled conditions and supplied with 0 or 40 μg chlorsulfuron L-1 . Zinc uptake rates of 12-d-old plants were measured over 80 or 90 minutes using65 Zn added to nutrient solutions. Increasing the zinc concentration of the solution increased the rate of zinc uptake, while the percentage of zinc transported to shoots was decreased. Addition of chlorsulfuron to uptake solutions for 90 minutes did not influence rate of zinc uptake or transport of zinc to shoots. Pretreating plants with chlorsulfuron for 5 days decreased zinc uptake rates, but transport to shoots was proportionally increased. Three-day pretreatment with chlorsulfuron was the minimum required for significant differences in uptake and transport of zinc to occur. Plants exposed to chlorsulfuron for 3 days required a further 5 days of growth in chlorsulfuron-free solutions before uptake rates recovered to control plant rates. It is concluded that chlorsulfuron deleteriously but reversibly affects uptake of zinc across the plasma membrane after prolonged exposure.
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    Plant and soil 197 (1997), S. 95-103 
    ISSN: 1573-5036
    Keywords: drought ; mycorrhizal fungi ; safflower ; water relations ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of arbuscular mycorrhizal fungi on drought tolerance and recovery was studied in safflower (Carthamus tinctorius L.) and wheat (Triticum aestivum L.). Plants were grown with and without the mycorrhizal fungus, Glomus etunicatum Becker & Gerd., in nutrient-amended soil under environmentally-controlled conditions to yield mycorrhizal and nonmycorrhizal with similar leaf areas, root length densities, dry weights, and adequate tissue phosphorus. When drought stress was induced, mycorrhizal infection did not affect changes in leaf water, osmotic or pressure potentials, or osmotic potentials of leaf tissue rehydrated to full turgor in either safflower or wheat. Furthermore, in safflower, infection had little effect on drought tolerance as indicated by the level of leaf necrosis. Mycorrhizal wheat plants, however, had less necrotic leaf tissue than uninfected plants at moderate levels of drought stress (but not at severe levels) probably due to enhanced phosphorus nutrition. To determine the effects of infection on drought recovery, plants were rewatered at a range of soil water potentials from –1 to –4 MPa. We found that although safflower tended to recover more slowly from drought after rewatering than wheat, mycorrhizal infection did not directly affect drought recovery in either plant species. Daily water use after rewatering was reduced and was correlated to the extent that leaves were damaged by drought stress in both plant species, but was not directly influenced by the mycorrhizal status of the plants.
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    Plant and soil 197 (1997), S. 79-86 
    ISSN: 1573-5036
    Keywords: dwarfing genes ; root and stem mass ratios ; root dry-weight ; root length ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of Rht alleles on root growth and distribution in isogenic lines of spring wheat (Triticum aestivum L.) are described under different environmental conditions. Above-ground biomass, root length, root dry-weight and their distribution along the soil profile were measured by destructive sampling for growth of aerial biomass and extraction of soil cores containing roots. Field experiments were conducted under non-limiting water and nutritional conditions during two consecutive years, using an early and a late sowing date each year. Dwarfing genes significantly reduced plant height and above-ground biomass at anthesis. In addition, stem mass ratio also was reduced with increases in the allelic dosage. Conversely, total root length and root dry-weight per unit area at anthesis were increased with decreased plant height, therefore, root mass ratio tended to be negatively correlated with plant height. Differences in distribution of root length and root dry-weight through the soil profile among lines were largely confined to the upper soil layers (i.e. the top 30 cm). Differences in root dry-weight were more important than in root length, so that the dwarf line had the highest root mass per unit root length. Furthermore, a significant positive correlation between the root mass ratio and stem mass per unit stem length was found. It is suggested that increases in root mass per unit root length associated with Rht alleles are evidencing a surplus of photoassimilates during stem elongation which are used for thickening the roots due to the lack of alternative sinks. Agronomic implications of this effect are discussed.
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    Plant and soil 190 (1997), S. 267-277 
    ISSN: 1573-5036
    Keywords: dynamic model ; root growth ; root impacts ; root-shoot ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The simulation of crop - soil systems with a model requires an appropriate description of the root dynamics. An empirical root growth model that simulates root-shoot relations, root distribution and a dynamic response to environmental conditions is presented. The root model extends an existing crop model and links it to a soil model to calculate dry matter accumulation, water and nitrogen dynamics of a wheat crop. Simulated roots are distributed over soil layers according to carbon supply from the shoots by using a 'top down principle'. This principle favours the top layers for root growth by first providing all available carbon to the first layer. Under unfavourable soil conditions in that layer, carbon is given to the next deeper soil layer. This procedure is repeated until a separately calculated rooting depth is reached. At that depth all available carbon is used for root growth regardless of current soil conditions. Under most simulated conditions the 'top down principle ' results in a negative exponential function of a monotone decrease of root distribution with soil depth. However, it can also account for larger root densities deeper in the profile when water or nitrogen deficiency occurs in soil. In addition to soil water and soil nitrogen supply the root model considers soil compaction, aeration and root distribution history for root growth simulation. The new model, consisting of an existing crop and soil model and linked through a new developed root model, was calibrated and tested using two independent field experiments. A sensitivity analysis was carried out by varying parameters, initial soil conditions and hypothetic weather patterns as part of the validation process. Root length density distribution (r2(1:1)=0.65), shoot, grain and total root biomass (r2(1:1)=0.87) were predicted satisfactorily, thus providing a useful tool for specific simulation studies on that site.
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  • 69
    ISSN: 1573-5036
    Keywords: 2 ; 4-D ; germination ; growth ; salinity-tolerance ; seed treatment ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Experiments were conducted under laboratory and greenhouse conditions to study the effect of 2,4-D on rooting and salinity tolerance of wheat. Seeds of one commercial wheat (Inqalab-91) and three salt-tolerant wheat lines (WL-41, WL-359, and WL-1073 developed through wide hybridization) were included in the study. Preliminary and short-term experiments were conducted to determine the level of 2,4-D (administered through seed soaking for 24.5 h. at 25 °C in the dark) at which the maximum number of roots emerged. Under hydroponic conditions, 2,4-D treatment of seeds caused an increase of 60 to 100% in the number of primary roots. The maximum increase in the number of roots was observed in one of the salt tolerant wheat lines (WL-41). The roots appeared in bunches but showed stunted growth at higher levels of 2,4-D. Dry matter accumulation decreased markedly; the effect was more pronounced in Inqalab-91 which is less tolerant to stress than other wheat lines. In all wheat types, allocation of dry matter to roots relative to shoot increased due to 2,4-D treatment. In soil, seeds treated with different levels of 2,4-D showed a germination delay of 1–3 days. Although the number of primary roots increased, 2,4-D treatment caused a decrease in total dry matter accumulation by plants grown for 40 days. In another experiment, conducted under greenhouse conditions, seed germination and growth of seedlings was significantly retarded in saline compared to that in non-saline (normal) soil. Initially, the pace of germination of treated seeds as well as seedling growth was slower in both soils, but after six weeks, the leaf area of seedlings raised from treated seeds was greater than those raised from untreated seeds. Towards maturity, plants arising from treated seeds developed wider and longer flag leaves leading to enhanced yield. Root biomass decreased in saline soil as compared to normal soil. However, 2,4-D treatment caused a substantial increase in root biomass in saline soil and the roots were harder in texture in wheats other than Inqalab-91. Seed treatment with 2,4-D led to a significant improvement in the number of productive tillers, yield of straw and grain, and grain protein content of all wheats grown in saline soil. Plants grown in normal soil did not show any marked effect of seed treatment on grain yield and other agronomic parameters. The four wheats showed substantial differences for different parameters but the salt tolerant wheat lines performed better compared to the commercial variety Inqalab-91.
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    Plant and soil 209 (1999), S. 283-295 
    ISSN: 1573-5036
    Keywords: leaf emergence ; phosphorus ; photosynthesis ; tillering ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Phosphorus (P) deficiency limits the yield of wheat, particularly by reducing the number of ears per unit of area because of a poor tiller emergence. The objectives of this work were to (i) determine whether tiller emergence under low phosphorus availability is a function of the availability of assimilates for growth or a direct result of low P availability, (ii) attempt to establish a quantitative relation between an index of the availability of P in the plant and the effects of P deficiency on tiller emergence, and (iii) to provide a better understanding of the mechanisms involved in tiller emergence in field-grown wheat. Wheat (Triticum aestivum L., cv. INTA Oasis), was grown in the field under drip irrigation on a typic Argiudol, low in P (5.5 μg P g-1 soil Bray & Kurtz I) in Balcarce, Argentina. Treatments consisted of the combination of three levels of P fertilization 0, 60 and 200 kg P2O5 ha-1, and two levels of assimilate availability, a control (non-shaded) and 65% of reduction in incident irradiance from seedling emergence until the end of tillering (shaded). Phosphorus treatments significantly modified the pattern of growth and development of the plants. Shading reduced the growth and concentration of water-soluble carbohydrates in leaves and stems. Leaf photosynthetic rate at saturating irradiance was reduced by P deficiency, but was not affected by shading. At shoot P concentrations less than 4.2 g P kg-1 the heterogeneity in the plant population increased with respect to the number of plants bearing a certain tiller. At a shoot P concentration of 1.7 g P kg-1 tillering ceased completely. Phosphorus deficiency directly altered the normal pattern of tiller emergence by slowing the emergence of leaves on the main stem (i.e. increasing the phyllochron), and by reducing the maximum rate of tiller emergence for each tiller.
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    Euphytica 107 (1999), S. 51-59 
    ISSN: 1573-5060
    Keywords: wheat ; plant breeding ; yield stability ; environmental index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of plant breeding on yield and their physiological determinants has been widely studied in wheat. However, it is poorly understood how, and to what extent, yield stability has been modified. To attempt a direct analysis of changes in absolute and relative yield stability, data of yield of cultivars released in different eras in different environments were obtained from records from our lab and from the literature. Depending on the availability of data, effects of plant breeding on yield stability of cultivars released in Argentina, Australia, Italy and the United Kingdom were evaluated using a quantitative approach. In this paper it was assumed that the slope of yield vs. environmental index estimates the instability of the cultivars. In addition, a more qualitative approach for Mexico, and the former USSR complemented this analysis. There was a clear decrease in yield stability assessed in absolute terms as a consequence of wheat breeding. In Argentina, Australia, Italy and the UK this decrease was related to the magnitude of yield increases. However, the decrease in yield stability in Argentina and Australia was less than for Italy and the UK, particularly so during the last 30 years. Modern cultivars released in Argentina and Australia showed a trend to maintain yield stability as a percentage of their yield similar to that of their predecessors, while the two European countries analysed tended to a slight decrease in yield stability even in relative terms. The complementary, less quantitative evaluation of Mexico and the former USSR appeared to confirm the quantitative trends described for the other countries, i.e. a general decrease in yield stability (assessed in absolute terms) with genetic gains in yield potential.
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  • 72
    ISSN: 1573-5060
    Keywords: chromatin ; breeding ; gel electrophoresis ; in situ hybridization ; rye ; rye-specific probes ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Chromosome arm 1RS of rye ( Secale cereale L.), when transferred to wheat ( Triticum sp.), significantly influences variety performance, because it carries genes for resistance to disease and insect pathogens. Inserted into wheat, 1RS also promotes haploid production, affects end-product quality, and sometimes affects yield. Therefore, its detection by breeders and geneticists is important. The entire 1RS arm is present in chromosome substitutions and in Robertsonian translocations involving chromosomes 1A, 1B, or 1D of wheat. In recombinant lines, a segment of 1RS has been exchanged with a segment of a group-1 wheat chromosome. Determining the wheat chromosome arm involved in a translocation, the source of rye chromatin, and the amount of 1RS chromatin introduced is necessary for a complete characterization of the introgressed segment. Biochemical, molecular, and cytogenetic technologies are described which enable such a characterization of 1RS in wheat. Examples of using gel electrophoresis, high-performance liquid chromatography, monoclonal antibodies, rye-specific molecular probes, RFLP and PCR assays, chromosome banding, in situ hybridization, and flow cytometry are provided. A comparison of these technologies is made and the advantages and disadvantages of each technology are discussed relative to modern wheat breeding efforts.
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  • 73
    ISSN: 1573-5060
    Keywords: Puccinia striiformis ; Triticum aestivum ; association of components ; stripe rust ; durable resistance ; infection frequency ; latency period ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Latency period, infection frequency, lesion length, lesion growth, disease severity and percentage of infected leaf parts were assessed on 10-day-old seedling leaves and flag leaves of ten bread wheat (Triticum aestivum L.) cultivars after inoculation with urediospores of Puccinia striiformis Westend. f. sp. tritici. For all components significant genotypic differences were detected. Components of resistance tended to be associated. A long latency period was associated with a low infection frequency, small lesions, a low disease severity and a low percentage of infected leaf parts. The latency period, measured as time period until first pustule appearance (LP1), was highly correlated with the latency period measured as time period until 50% of the pustules appeared (LP50). Assessment of latency period of large numbers of cultivars could therefore be reliably done by measuring LP1 which is less time consuming than measuring LP50. Latency period, infection frequency and disease severity were highly correlated with disease development data from field experiments. These results suggest that selection in the greenhouse for one of these components should result in cultivars with high levels of quantitative resistance. Disease severity after uniform inoculation in the greenhouse can be used for monocyclic evaluations because it is the easiest to assess.
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  • 74
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    Euphytica 97 (1997), S. 39-44 
    ISSN: 1573-5060
    Keywords: Agropyron ; Elymus ; Fusarium head blight ; scab ; resistance ; genetic resources ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Four species of indigenous Japanese Agropyron, namely, Elymus humidus Osada (= Agropyron humidum), E. tsukushiensis Honda var. transiens (= A. tsukushiense), E. racemifer Tsvel. (= A. ciliare) and A. mayebaranum var. intermedium Hatusima were collected and evaluated for their resistance to Fusarium head blight (FHB, scab) after inoculation with a conidial suspension of Fusarium graminearum Schwabe at the flowering stage. The resistance to penetration of FHB and to fungal invasion of rachis internodes was evaluated in each accession in a comparison with six wheat cultivars. The results demonstrated that AG.91-35 of E. humidus and AG.91-24 of E. racemifer had higher resistance to penetration than the resistant wheat cultivars Nobeokabouzu-komugi and Sumai 3, respectively. All accessions of indigenous Japanese species of Agropyron examined, with the exception A. mayebaranum, had a statisticallysimilar resistance to invasion, which was the same as that of Nobeokabouzu-komugi or Sumai 3. In all accessions of E. humidus, with only one exception, no spread of the fungus from the infected spikelets to the rachis internodes was detected at all. The possible application of the strains of Agropyron as genetic resources for development of resistance to FHB in wheat is discussed.
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  • 75
    ISSN: 1573-5060
    Keywords: dwarfing genes ; wheat ; radiation interception ; radiation use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three near isogenic lines of spring wheat grown to determine whether Rht dwarfing alleles alter radiation interception. A field study (involving two sowing dates in two growing seasons) with lines containing different allelic dosage of Rht1 and Rht2 (i.e. dwarf, DD; semi-dwarf, SD and tall SH), was conducted without water and nutritional deficiencies. Dwarfing genes did not modify the timing of occurrence of phenological events. Above-ground biomass at anthesis was reduced by 22% in the DD line in relation of the rest of the lines. However, at maturity accumulated biomass of the DD and SH lines were not significant different. Dwarfing genes increased the light attenuation coefficient (k, with values of 0.48, 0.62 and 0.78 for the SH, SD and the DD line respectively). A similar trend was followed to the leaf thickness (estimated by the specific leaf weight, SLW). Despite the differences observed among the lines, both in k and SLW values, they did not differ significantly in the proportion of incoming radiation intercepted by the canopy, nor in the cumulative intercepted radiation during the pre and post-anthesis periods. Radiation use efficiency (RUE) differed significantly among the lines. While RUE during pre-anthesis was the lowest in the DD line, from anthesis to maturity the lines with Rht alleles showed higher RUE values than the SH line. The low pre-anthesis RUE in the DD lines could be associated with (i) poor canopy architecture due to reductions in leaf sheath and internode lengths and/or (ii) reduced canopy CO2 exchange rate. Post-anthesis RUE was lower than that recorded pre-anthesis in all lines. But the magnitude of the reduction was inversely related to the doses of the Rht alleles. Post-anthesis RUE appeared to be closely and positively associated with the number of grains set per unit biomass at anthesis. This relationship suggests a regulatory effect of the sink size on the efficiency of the crop to convert radiation into biomass during this period.
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  • 76
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    Plant molecular biology 40 (1999), S. 921-933 
    ISSN: 1573-5028
    Keywords: A23187 ; calcium ; elicitor ; MAP kinase ; Typhula ishikariensis ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat cultured cells were used to study the role of Ca2+ in regulating protein kinases during the induction of defense-related genes by fungal elicitor treatments. Manipulation of intracellular Ca2+ concentrations by treatment with calcium ionophore A23187 in the presence of high extracellular Ca2+ resulted in the induction of mRNA expression of WCK-1, a gene encoding mitogen-activated protein (MAP) kinase. The induction of WCK-1 mRNA by A23187 did not occur when extracellular Ca2+ was chelated by 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA). The WCK-1 mRNA was also induced by Typhula ishikariensis-derived elicitors, suggesting a possible involvement of WCK-1 in the plant defense response against pathogens. BAPTA and a calcium channel blocker, La3+, inhibited the elicitor-induced expression of the WCK-1 mRNA. A recombinant fusion protein of WCK-1 (GST-WCK-1) autophosphorylated at the Tyr residue and exhibited an autophosphorylation-dependent protein kinase activity towards myelin basic protein. Alteration of Tyr-196 in the conserved ‘TEY’ motif in GST-WCK-1 to Phe by site-directed mutagenesis abolished the autophosphorylation. The GST-WCK-1 protein was activated by elicitor-treated wheat cell extracts but not by the control extract. These results suggest that fungal elicitors activate WCK-1, a specific MAP kinase in wheat. Furthermore, the results suggest a possible involvement of Ca2+ in enhancing the MAP kinase signaling cascade in plants by controlling the levels of the MAP kinase transcripts.
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  • 77
    ISSN: 1573-5028
    Keywords: alternative splicing ; starch biosynthesis ; starch-branching enzyme ; transit peptide ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A wheat gene, denoted Sbe1, encoding a type I starch-branching enzyme (SBEI) was isolated from a genomic library and shown to comprise 14 exons distributed over a 5.7 kb DNA region. Analyses of kernel RNA by 5′ rapid amplification of cDNA ends (5′-RACE) and reverse transcription-polymerase chain reaction (RT-PCR) demonstrated a considerable sequence variation at the 5′ ends of SBEI gene transcripts. DNA sequence alignments between the 5′-RACE products and the Sbe1 genomic DNA indicated that the first two exons and first intron were differentially processed to generate three classes of the mature transcript. One form of the SBEI gene transcript in 12-day old kernels contained the exon I+II+III combination at the 5′ end, whereas other forms differed by inclusion of intron 1 or exclusion of exon II sequences. RT-PCR analysis of Sbe1-uidA::nptII chimeric mRNA produced in transgenic wheat cultured cells confirmed that the isolated Sbe1 was able to produce all three forms of SBEI gene transcripts by alternative splicing of the primary mRNA. The variants of processed Sbe1 mRNA were potentially translated into N-terminal variants of the SBEI precursor with different transit peptide sequences.
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  • 78
    ISSN: 1573-5036
    Keywords: boron ; rice-wheat rotation ; sterility ; time of sowing ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spikelet sterility in wheat (Triticum aestivum L.) is emerging as a production threat in different parts of Nepal. This study was aimed at determining the effects of sowing date and boron application in controlling spikelet sterility in four different genotypes of spring wheat in a rice-wheat system in the western hills of Nepal. Four genotypes of known different responses to boron were planted on 21 November, 6 December and 21 December, 1994 with or without boron application at 1 kg B ha-1 (i.e. 9 kg borax ha-1) on a soil that was known to be deficient in boron. The effect of sowing date was significant for the phenology, yield components, percentage sterility and grain yield. Sterility was significantly increased in the crop planted on 21 December, which had also the lowest 1000 seed weight and grain yield; there was an almost 50% grain yield reduction compared to the crop planted on 21 November. Terminal moisture stress (i.e. lack of moisture during the later part of the development) was observed in the late sown crop which also amplified the extent of sterility associated with boron deficiency. Genotypes differed in response to sowing dates and boron treatment for all of the phenological events measured, yield components, grain yield and percentage sterility. SW-41 and BL-1022 had significantly higher sterility at all sowing dates. BL-1249 showed a consistently lower% sterility over all sowing dates and boron treatments. The addition of boron significantly increased the number of grains set per spike thereby decreasing the total sterility in boron responsive genotypes SW-41 and BL-1022 while those not susceptible did not respond. The boron concentration in the flag leaf at anthesis was increased in treatments with added B in the soil but genotypes did not differ in boron concentration for any soil treatment.
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  • 79
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    Plant and soil 208 (1999), S. 149-159 
    ISSN: 1573-5036
    Keywords: abscisic acid ; drought ; soil water content ; water potential ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to investigate the effects of soil texture on possible non-hydraulic signals under field conditions, spring wheat plants (Triticum aestivum L. cv. Cadensa) grown in sand and loam soils and with a well developed root system were exposed to slow soil drying in the late vegetative stage of growth. Soil water potential and content were measured daily at different depths and plant responses were measured in flag leaves. When the average soil water potential in the top soil layers (0–25 cm depth in sand and 0–45 cm depth in loam) dropped to –60 or –70 kPa and the lower soil layers were still at field capacity, morning xylem [ABA] (0.03–0.04 vs. 0.06–0.08 mmol m-3) and midday leaf ABA concentration increased (250–300 vs. 400–450 ng/g DW) and leaf conductance decreased relatively to well-watered (control) plants (0.75–0.88 vs. 0.64–0.70 mol m-2 s-1). These responses took place before any decrease in leaf water potential occurred as compared with control plants, indicating that they were triggered by root-borne signals due to reduced root water status in the top soil layers. At this stage the soil water content was as low as 6% by volume, the fraction of roots in ‘wet’ soil was 0.12 and relative available soil water was 45% in sand and still high 20%, 0.48 and 70%, respectively, in loam of the whole soil profile indicating that roots were responding to soil water availability and not soil water content at a certain evaporative demand. In addition, similar responses occurred at high and low evaporative demands (3.4–5.2 vs. 0.6–4.0 mm/day of potential evapotranspiration).
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  • 80
    ISSN: 1573-5036
    Keywords: macropores ; rhizosphere ; roots ; root-soil interplay ; soil properties ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Under certain soil conditions, e.g. hardsetting clay B-horizons of South-Eastern Australia, wheat plants do not perform as well as would be expected given measurements of bulk soil attributes. In such soils, measurement indicates that a large proportion (80%) of roots are preferentially located in the soil within 1 mm of macropores. This paper addresses the question of whether there are biological and soil chemical effects concomitant with this observed spatial relationship. The properties of soil manually dissected from the 1–3 mm wide region surrounding macropores, the macropore sheath, were compared to those that are measured in a conventional manner on the bulk soil. Field specimens of two different soil materials were dissected to examine biological differentiation. To ascertain whether the macropore sheath soil differs from rhizosphere soil, wheat was grown in structured and repacked cores under laboratory conditions. The macropore sheath soil contained more microbial biomass per unit mass than both the bulk soil and the rhizosphere. The bacterial population in the macropore sheath was able to utilise a wider range of carbon substrates and to a greater extent than the bacterial population in the corresponding bulk soil. These differences between the macropore sheath and bulk soil were almost non-existent in the repacked cores. Evidence for larger numbers of propagules of the broad host range fungus Pythium in the macropore sheath soil were also obtained.
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  • 81
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    Plant and soil 215 (1999), S. 65-72 
    ISSN: 1573-5036
    Keywords: barley ; boron deficiency ; Hordeum vulgare ; Triticum aestivum ; variation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Responses of a range of barley (Hordeum vulgare L.) genotypes to boron (B) deficiency were studied in two experiments carried out in sand culture and in the field at Chiang Mai, Thailand. In experiment 1, two barley genotypes, Stirling (two-row) and BRB 2 (six-row) and one wheat (Triticum aestivum L.) genotype, SW 41, were evaluated in sand culture with three levels of applied B (0, 0.1 and 1.0 μM B) to the nutrient solution. It was found that B deficiency depressed flag leaf B concentration at booting, grain number and grain yield of all genotypes. In barley Stirling, B deficiency also depressed number of spikes plant-1, spikelets spike-1 and straw yield. However, no significant difference between genotypes in flag leaf B concentration was found under low B treatments. Flag leaf B concentration below 4 mg kg-1 was associated with grain set reduction and could, therefore, be used as a general indicator for B status in barley. In experiment 2, nine barley and two wheat genotypes were evaluated in the field on a low B soil with three levels of B. Boron levels were varied by applying either 2 t of lime ha-1 (BL), no B (B0) or 10 kg Borax ha-1 (B+) to the soil prior to sowing. Genotypes differed in their B response for grain spike-1, grain spikelet-1 and grain set index (GSI). The GSI of the B efficient wheat, Fang 60, exceeded 90% in all B treatments. The B inefficient wheat SW 41 and most of the barley genotypes set grain normally (GSI 〉80%) only at the B+. In B0 GSI of the barley genotypes ranged from 23% to 84%, and in BL from 19% to 65%. Three of the barley with severely depressed GSI in B0 and BL also had a decreased number of spikelets spike-1. In experiment 3, 21 advanced barley lines from the Barley Thailand Yield Nursery 1997/98 (BTYN 1997/98) were screened for B response in sand culture with no added B. Grain Set Index of the Fang 60 and SW 41 checks were 98 and 65%, respectively, and GSI of barley lines ranged between 5 and 90%. One advanced line was identified as B efficient and two as moderately B efficient. The remaining lines ranked between moderately inefficient to inefficient. These experiments have established that there is a range of responses to B in barley genotypes. This variation in the B response was observed in vegetative as well as reproductive growth. Boron efficiency should be considered in breeding and selection of barley in low B soils.
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  • 82
    ISSN: 1573-5036
    Keywords: chelator ; genotypic differences ; HEDTA ; ion speciation ; micronutrient ; tolerance to zinc deficiency ; wheat ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The chelator-buffered nutrient solutions containing excess chelator have been used frequently in the micronutrient research, but potential toxicity of the excess chelator has not been ascertained. The present study was conducted to test effects of four concentrations of excess HEDTA [ N-(2-hydroxyethyl)ethylenedinitrilotriacetic acid] and two levels of total Zn on growth, root exudation, and nutrient uptake and transport by Triticum aestivum L. (cv. Aroona) and Triticum turgidum L. conv. durum (Desf.) MacKey (cv. Durati) genotypes differing in tolerance to Zn deficiency. Excess HEDTA at 50 μM reduced root and shoot growth and caused visual toxicity symptoms (necrotic lesions) on leaves; these effects were generally absent at lower concentrations of excess HEDTA. Root exudation of phytosiderophores increased with increasing concentrations of excess HEDTA at deficient and sufficient Zn levels, and was higher in Zn-deficiency-tolerant Aroona than in Zn-deficiency-sensitive Durati wheat. Shoot and root Zn concentrations showed a saturable response to increasing Zn2+ activities in solution. Excess HEDTA at 50 μM caused an increase in shoot concentrations of Fe and a decrease in concentrations of Mn and Cu. An average rate of Zn uptake increased with an increase in Zn2+ ionic activity in solution, with Zn-deficiency-tolerant Aroona having a higher rate of Zn uptake than Zn-deficiency-sensitive Durati in the deficiency range of Zn2+ activities. Average uptake rates of Mn and Cu decreased with an increase in concentration of excess HEDTA. Similar observations were noted for transport of Mn and Cu to shoots, while Zn transport to shoots was proportional to Zn2+ activities in solution. It was concluded that excess HEDTA at 50 μM adversely affects wheat growth and physiology, while excess of 25 μM or less does not cause measurable toxicity.
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  • 83
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    Plant molecular biology 40 (1999), S. 567-578 
    ISSN: 1573-5028
    Keywords: wheat ; mitochondria ; RNA polymerase ; transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using PCR-based methods, we assembled two wheat cDNA sequences, wheat-G and wheat-C, that encode T3/T7 bacteriophage-like RNA polymerases (RNAPs) sharing 45% amino acid identity. In phylogenetic analyses using maximum likelihood, parsimony and distance methods, the predicted protein sequence of wheat-G (1005 amino acids, 113 kDa) clusters with sequences of previously assigned mitochondrial RNAPs from dicotyledonous plants (Arabidopsis thaliana, Chenopodium album); likewise, in such analyses, the wheat-C sequence (949 amino acids, 107 kDa) affiliates specifically with the Arabidopsis sequence that encodes a phage-like RNAP thought to function in chloroplasts. To confirm biochemically the assignment of the gene encoding the putative wheat mitochondrial RNAP, we isolated a ca. 100 kDa wheat mitochondrial protein that is enriched in fractions displaying specific in vitro transcription activity and that reacts with an antibody raised against a recombinant maize phage-type RNAP. Internal peptide sequence information obtained from the 100-kDa polypeptide revealed that it corresponds to the predicted wheat-G cDNA sequence, providing direct evidence that the wheat-G gene (which we propose to call RpoTm) encodes the wheat mitochondrial RNAP.
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  • 84
    ISSN: 1573-5036
    Keywords: arbuscular mycorrhiza ; hydroponics ; linseed ; sorghum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Linum usitatissimum, Sorghum bicolor and Triticum aestivum plants were further colonised by the arbuscular mycorrhizal fungus, Glomus mosseae, during a four week period of hydroponic culture after a pre-culture period of three weeks with the fungus in perlite substrate. The viability of mycorrhizal colonisation of T. aestivum was indicated by an initial experiment where G. mosseae from mycorrhizal plants colonised non-mycorrhizal plants when the plants were grown together in the same hydroponic container using modified Long Ashton nutrient solution. Intermittant aeration of the plant roots (2 h periods, four times per day) provided a compromise between adequate aeration and minimal disturbance of the fungus. In a second experiment, two nutrient media, modified Long Ashton and modified Knop plus Hoagland medium were compared for culturing G. mosseae on T. aestivum. A significantly higher root dry weight was found for the mycorrhizal versus the non-mycorrhizal wheat plants in modified Long Ashton nutrient medium, which contained 10 µM P and an organic buffer. Modified Knop plus Hoagland nutrient medium contained a high P concentration (0.9 mM) and did not produce viable cultures of mycorrhizal colonisation. In a third experiment, modified Long Ashton medium was used for hydroponic culture of mycorrhizal L. usitatissimum, S. bicolor and T. aestivum. The root colonisation percentages for T. aestivum (73%), S. bicolor (36%) and L. usitatissimum (65%) were within the range of colonisation rates obtained with solid substrate culture in perlite. Viability of the mycorrhizal structures in hydroponic culture was assessed by monitoring activity of fungal succinate dehydrogenase and found to be similar to cultures in perlite. No difference in the P concentration of mycorrhizal and non-mycorrhizal plants was observed, possibly owing to the lack of diffusion limits for P in hydroponic solution. This report describes a system for the viable culture of G. mosseae with different plant species where a high mycorrhizal colonisation rate was produced under conditions of a short culture period using intermittent aeration, a low concentration of P supply and an organic buffer.
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  • 85
    ISSN: 1573-5036
    Keywords: agriculture ; fertilisation ; nitric oxide flux ; nitrification ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The surface flux of nitric oxide from a wheat field was investigated from 23 March to 29 May 1997 in the Kerzersmoos, Switzerland. A plot fertilised with 19 kg N ha-1 in cattle slurry and 40 kg N ha-1 in mineral NH4NO3 fertiliser and a plot receiving no nitrogen containing fertiliser were compared. The flux was calculated based on hourly measurements of the NO soil–atmosphere concentration gradient using the one-dimensional soil diffusion model of Galbally and Johansson (1989). The soil bulk diffusion coefficient was determined from measurements of the 222Rn surface flux and the activity gradient between 10 cm depth and the surface. It ranged between 79% and 0.3% of the NO diffusion coefficient in air and was parameterised by air filled soil pore space. The indirectly determined NO flux agreed well with standard flux measurements using dynamic chambers. The largest NO emission was found following fertiliser application and irrigation. The emission occurred in pulses, which lasted for 4 days up to 3 weeks coinciding with elevated soil ammonium concentrations. Nitric oxide emission in 5 days following application of cattle slurry were 31 g NO-N ha-1 and 5 g NO-N ha-1 from the non-fertilised plot, respectively. Nitric oxide emission in 15 days following application of NH4NO3 was 95 g NO-N ha-1 and 10 g NO-N ha-1 from the non-fertilised plot, respectively. NO emission in 4 days following irrigation on 21 April were 36 g N ha-1 from the fertilised and 39 g N ha-1 from the non-fertilised plot. The daily NO emission before and after fertiliser and irrigation pulses was between 0.3 and 0.7 g NO-N ha-1 d-1. NO production and NO uptake of the soil was measured regularly. No systematic influence of management or climate on NO uptake was found. NO production was strongly stimulated by fertiliser input and soil moisture content. The simulation of NO production could be reproduced using a nitrification algorithm (Riedo et al., 1998) driven by soil temperature, moisture and ammonium concentration. A NO production rate constant of 1.1ċ10-3 h-1 at 15 °C was derived from a linear regression between nitrification and NO production. Introducing the parameterisation of NO production into the model of Galbally and Johansson (1989) the duration and the strength of the NO emission pulses could be reproduced and the total NO emission during the experiment was approximated within a factor of two.
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    Plant and soil 211 (1999), S. 223-230 
    ISSN: 1573-5036
    Keywords: silicon absorption ; transport ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Although silicon (Si) is a quantitatively major inorganic constituent of higher plants the element is not considered generally essential for them. Therefore it is not included in the formulation of any of the solution cultures widely used in plant physiological research. One consequence of this state of affairs is that the absorption and transport of Si have not been investigated nearly as much as those of the elements accorded 'essential' status. In this paper we report experiments showing that Si is rapidly absorbed by wheat (Triticum aestivum L.) plants from solution cultures initially containing Si at 0.5 mM, a concentration realistic in terms of the concentrations of the element in soil solutions. Nearly mature plants (headed out) 'preloaded' with Si absorbed it at virtually the same rate as did plants grown previously in solutions to which Si had not been added. The rate of Si absorption increased by more than an order of magnitude between the 2-leaf and the 7-8 leaf stage, with little change thereafter.
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  • 87
    ISSN: 1573-5060
    Keywords: barley ; embryogenesis ; medium ; regeneration ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Media have been developed for somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum. For wheat and tritordeum inflorescences, regeneration from embryogenic calluses induced on medium with picloram was almost twice as efficient as regeneration from cultures induced on 2,4-dichlorophenoxyacetic acid (2,4-D). The addition of zeatin at 5 or 10 mg l−1 to regeneration media had a positive effect on regeneration. For scutella, the highest frequencies of embryogenesis (85%) and regeneration (50%) was obtained using an induction medium containing 2 mg l−1 of 2,4-D and half concentration of aminoacids. The morphogenetic capacities of 19 different cultivars of wheat, barley and tritordeum were compared, and clear differences were found both between explants and genotypes. In wheat, embryogenic capacity from inflorescences (average of 92%) was higher than from immature scutella (average of 62%). However, shoot regeneration from scutella was clearly higher than from inflorescences (averages of 63%, and 18% respectively). Frequencies of regeneration in wheat and barley varied widely among the cultivars tested and in both species no difference was found between spring and winter varieties.
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    Euphytica 108 (1999), S. 193-198 
    ISSN: 1573-5060
    Keywords: K+/Na+ selectivity ; Lophopyrum elongatum ; salt-stress ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Accumulation of potassium ions (K+) in expanding or most recently expanded leaves and exclusion of sodium ions (Na+) from them (K+/Na+ selectivity) have been shown to be associated with salt stress tolerance in wheat and Lophopyrum elongatum, a highly salt stress tolerant relative of wheat. This physiological trait is expressed in an amphiploid from the cross of wheat (cv. Chinese Spring) × L. elongatum and the chromosomes controlling it have been identified in field studies employing Chinese Spring disomic substitution lines with individual L. elongatum chromosomes. In this paper the arm location of these genes was investigated by assessing K+/Na+ selectivity in lines harboring individual chromosomes and chromosome arms of L. elongatum. Lophopyrum elongatum chromosome arms 1ES, 7ES, and 7EL, were shown to enhance K+/Na+ selectivity in wheat.
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  • 89
    ISSN: 1573-5060
    Keywords: diallel analysis ; Fusarium culmorum ; heterosis ; resistance ; scab ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fusarium head blight (FHB, scab) caused by Fusarium spp. is a widespread disease of cereals causing relevant yield and quality losses and contaminating cereal products with mycotoxins. Breeding resistant cultivars is the method of choice for controlling the disease. Resistance to FHB is a quantitative trait and is most likely governed by several genes. We present the results of an F1 diallel analysis of FHB resistance involving six resistant and one susceptible European winter wheat genotypes of diverse origin in order to identify promising combinations for the selection of improved cultivars. Parents and F1s including reciprocals were evaluated for FHB resistance in an artificially inoculated field trial. Two traits were assessed: visual disease symptoms on the heads and the percentage of Fusarium damaged kernels in a harvested sample. General combining ability (GCA) and specific combining ability (SCA) effects were statistically significant for visual symptoms and kernel damage, whereas reciprocal effects were small or not significant. Heterosis for resistance was common, indicating that the parental genotypes possess different resistance genes. Selection of transgressive segregates should be feasible from such heterotic combinations.
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  • 90
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Hordeum vulgare ; wheat ; barley ; chromosome addition lines ; translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two disomic barley chromosome addition lines and five translocated chromosome addition lines of common wheat cultivar Shinchunaga were isolated. They were derived from a hybrid plant between Shinchunaga and cultivated barley Nyugoruden (New Golden) by backcrossing with wheat and self pollination. Barley chromosomes added to chromosome arms involved in the translocated chromosomes were identified by C-banding method and by crossing these lines with Chinese Spring/Betzes addition lines. Two disomic addition lines were identified to have chromosome 6 and 7 of barley, respectively. Two of the five translocated chromosome addition lines were clarified to have same chromosome constitution, 42 wheat chromosomes and a pair of translocated chromosomes constituted with a long arm of chromosome 5B of wheat and a short arm of chromosome 7 of barley. The other three lines could not be identified due to chromosome rearrangement. Performances of these seven lines on agronomic characters were examined. Addition of barley chromosome 7 induced early heading, and chromosome 6 showed lated heading. Almost all of the lines except that of chromosome 6 showed short culm length and all showed reduced number of tillers, spikelets and grains per ear, and low seed fertility. These lines would be useful for genetic analyses in wheat and barley and for induction of useful genes of barley into wheat.
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  • 91
    ISSN: 1573-5060
    Keywords: clustering procedure ; genotype × environment interaction ; multi-trait analysis ; quality ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Structure of genotype × environment interaction was studied in two series of trials for three quality traits in bread wheat. Two kinds of environments were present in each series of trials: macro-environments defined as locations or location × year combinations and micro-environments induced by diversified cultural practices within each site. For each trait, a simultaneous clustering procedure was used to identify groups of environments which were homogeneous for interaction. An optimised series of trials was proposed from the clusters obtained. The cultural practice based on nitrogen fertilisation seemed to better diversify environments for interaction than use of fungicide, when all quality traits were considered. Determining an optimised series of trials simultaneously for the three traits led to keeping more environments than when one trait was considered. Suggestions for establishing a series of trials for a multi-trait analysis were proposed.
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  • 92
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    Euphytica 93 (1997), S. 353-360 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Secale cereale ; powdery mildew ; Erysiphe graminis f.sp. tritici ; resistance ; suppression ; segregation distortion ; wheat ; rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The location of wheat gene Pm8 for resistance to powdery mildew in the 1RS chromosome segment derived from cereal rye cultivar Petkus was confirmed. There was reduced transmission of the 1BL.1RS chromosome relative to its wheat homologue in four of the five crosses examined. Pm8 was not expressed in some wheat cultivars and selections which possessed the 1RS chromosome identified by the presence of relevant genes for resistance to the three rusts, due to the presence of a dominant suppressor gene(s) in the wheat genome. The frequency of suppression in 1BL.1RS wheats from Mexico was significantly higher than in a group of wheats (both local and exotic) introduced from China and was probably much higher than in European wheats.
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  • 93
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    Euphytica 94 (1997), S. 31-36 
    ISSN: 1573-5060
    Keywords: Triticum ; Fusarium graminearum ; resistance ; head scab ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Multi-floret and single-floret injection inoculation methods were used to test 1076 accessions of Triticum for resistance to initial infection and resistance to pathogen spread within spike tissue respectively. The data obtained between years or inoculation dates were comparatively similar and were little influenced by climatic factors, especially humid condition. All the tested materials uniformly exhibited susceptibility to initial infection, but there were great differences in resistance to spread among species or cultivars. The diploid and tetraploid wheats were severely susceptible. Only 30 genotypes of common wheat of T. aestivum concv. vulgare showed high resistance to spread. The highest frequency of high spread resistance existed in these landraces which were distributed in Zhejiang, Hubei, Hunan, Jiangsu provinces and Shanghai City, Guizhou, Sichuan and Yunnan provinces ranked second. The majority of wheat landraces from Hebei, Shanxi, Shaanxi Province and Beijing City showed susceptibility or high susceptibility. No resistance was found in Tibetian wheats.
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  • 94
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    Euphytica 94 (1997), S. 279-286 
    ISSN: 1573-5060
    Keywords: Triticum turgidum ; T. monococcum ; T. boeoticum ; T. urartu ; wheat ; Puccinia striiformis ; stripe rust ; rust resistance ; amphiploid ; suppressors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Stripe rust (caused by Puccinia striiformis Westend.) is a wheat disease of worldwide importance. Seedlings of 75 accessions of Triticum boeoticum, 12 of T. monococcum, 16 of T. urartu, 230 of durum wheat (T. turgidum L. var. durum), and 128 amphiploids (genome AAAABB) involving the crosses of the three diploid species (AA) with T. turgidum (AABB) were evaluated in the greenhouse for their reaction to P. striiformis race 14E14. Durum wheats and the amphiploids were also evaluated at two field locations in Mexico with the same race for their adult plant response. Resistant seedling reactions (infection types: 0-3 on a 0-9 scale) were seen for 10 (13%) accessions of T. boeticum, 19 (8%) accessions of T. turgidum and 32 (25%) amphiploids. The remaining accessions were either moderately resistant (ITs 4-6) or susceptible (ITs 7-9). The three amphiploids derived from the crosses of seedling resistant T. boeoticum and T. turgidum, were resistant as seedlings. Among the 51 amphiploids involving one resistant parent, 29 were resistant and the remaining 22 displayed intermediate to susceptible reactions. Suppressors for resistance were common in the A and AB genomes and suppression was resistance gene specific. Forty-five (20%) durums showed adequate field resistance (relative AUDPC 〈10% of the susceptible check ‘Morocco’). These included the 19 seedling resistant durums. Presence of genes involved in adult plant resistance was evident, because 26 of the remaining adult plant resistant durums had displayed intermediate-susceptible seedling reactions. Though the seedling reactions of the amphiploids varied from low to high, all involving the adult plant resistant durums possessed adequate field resistance. The resistant, newly produced, AAAABB amphiploids are useful genetic resources for stripe rust resistance which could be transferred to the cultivated T. turgidum.
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  • 95
    ISSN: 1573-5060
    Keywords: Israel ; landraces ; Puccinia recondita ; Puccinia striiformis ; disease resistance ; wheat ; Triticum spp. ; leaf rust ; yellow rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The purpose of this study was to identify the species of local landraces of wheat (Triticum spp.), held in the Israel Gene Bank, to evaluate them for basic characters and to assess their response to infection by two rust fungi under artificial inoculation conditions. One-hundred-thirty one seed samples were collected from local or Beduin farmers during 1978–1981 throughout the Galilee, Mt. Gilboa. Judean Desert and the south Negev. The samples were collected and stored in the Israel Gene Bank without any characterization or evaluation. Each accession was planted in a 1 m row at Bet Dagan and grown under favorable conditions for plant growth and rust development. Determination of the species, data of plant height, days to heading and reaction of the landraces to artificial inoculation with a composite inoculum of Puccinia recondita and P. striiformis were collected from each row. A small part of the landraces collection consisted of mixed populations of T. durum and T. aestivum plants, where one of the two species was predominant. One-hundred-fourteen and 17 accessions from this collection represented, respectively, Triticum durum and T. aestivum Israel landraces. Large variations were found for all the characters examined. Of the total accessions, 6.5% (8 accessions) and 32% (42 accessions) were resistant, respectively, to yellow- and leaf-rust. It was concluded that the diversified populations of the local landraces of wheat can be used as a source not only for genes affecting basic characters such as plant height and heading date, but also for resistance to leaf rust and yellow rust.
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  • 96
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    Euphytica 94 (1997), S. 257-261 
    ISSN: 1573-5060
    Keywords: centric breakage and fusion ; translocation ; substitution ; wheat ; rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Complete chromosomes 1R and 1B were reconstructed in wheat from the centric wheat-rye translocation 1RS.1BL. Three substitutions: 1R(1A), 1R(1B), 1R(1D), and three new centric translocations: 1RS.1AL, 1RS.1BL, 1RS.1DL were produced from the reconstructed chromosome 1R. Each one of these has the same rye chromosome arm 1RS which was present in the original translocation 1RS.1BL of ‘Kavkaz’ wheat. Reconstructed chromosome 1B and a normal chromosome 1R were used to produce a new 1RS.1BL translocation. This translocation has the long arm from the original 1RS.1BL translocation of ‘Kavkaz’, but a different 1RS arm. The third generation centric translocations were mitotically stable and were normally transmitted to progeny. Misdivision frequency of the reconstructed chromosomes 1R did not change relative to normal 1R, whereas the misdivision frequency of the two reconstructed chromosomes 1B tested was significantly higher relative to normal 1B. These experiments demonstrate that repeated cycles of centric breakage and fusion do not impair the function of centromeres in wheat and rye but may change chromosome's susceptibility to misdivision.
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  • 97
    ISSN: 1573-5060
    Keywords: Boron ; genotypic variability ; sterility ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two field experiments were carried out at the Khairanitar Farm, Nepal during the winter season of 1993 to investigate whether wheat genotypes differ in their sterility response to low soil boron and whether boron added to soil can correct sterility. A nursery consisting of 41 diverse genotypes from Nepal, India, Bangladesh, Thailand, China, Pakistan and CIMMYT/Mexico were evaluated in a plot where severe sterility had been observed in previous years. The second experiment involved six genotypes of known sterility responses to boron grown with or without an application of boron at 1 kg ha-1. Large differences were observed among genotypes in terms of number of grain set per ear (range 0.5 to 30 ) and sterility (5.5% to 97.5%). About one third of all the genotypes had 〈25% sterility and 16 of them suffered 〉75% sterility. The genotypes Fang-60, Sonalika, De Mai 6-22, BUC/FLK/MYNA/VL and HDW-234 were highly tolerant to boron deficiency with 〈10% sterility. In sharp contrast, genotypes BOW/BUT, SERI/THB, Glennson, SW-41, Yunmai-33 and UHU were highly susceptible and set virtually no grains in the boron deficient plots. Strong responses to boron application were observed and genotypic variations were evident. Boron at 1 kg ha-1significantly reduced the number of late ears, increased number of grains per ear and grain yield in boron-responsive genotypes. Tolerant genotypes were not influenced by added boron. Given these striking genetic differences, progress in breeding wheat for B-deficient soils in Nepal should be worthwhile.
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  • 98
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    Plant growth regulation 28 (1999), S. 187-197 
    ISSN: 1573-5087
    Keywords: ABA ; grain filling ; water stress ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of in situ water stress on the endogenous abscisic acid (ABA) content of the endosperm and the in vitro application of ABA on some important yield regulating processes in wheat have been studied. Water stress resulted in a marked increase in the ABA content of the endosperm at the time close to cessation of growth. Application of ABA to the culture medium of detached ears reduced grain weight. Exogenously applied ABA, at the highest concentration (0.1 mM) reduced transport of sucrose into the grains and lowered the starch synthesis ability of intact grains. In vitro sucrose uptake and conversion by isolated grains was stimulated by low ABA concentrations (0.001 mM) in the medium but was inhibited by higher concentrations. ABA application had no effect on sucrose synthase (SS) and uridine diphosphate glucose pyrophosphorylase (UDP-Gppase) activities, whereas adenosine diphosphate glucose pyrophosphorylase (ADP-Gppase), soluble starch synthase (SSS), and granule-bound starch synthase (GBSS) activities were reduced. These results raise the possibility that water stress-induced elevated levels of endogenous ABA contribute to reduced grain growth.
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  • 99
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    Plant growth regulation 29 (1999), S. 1-21 
    ISSN: 1573-5087
    Keywords: drought resistance ; wheat ; small grains ; genetic analysis ; yield stability ; traits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Drought is a serious problem in many parts of the world where wheat, barley and other small-grained cereals are part of the staple diets. Even in parts of South-eastern Europe, seasonal rainfall for winter cereals has been falling gradually for many years. Thus, since 1981 across three sites in Yugoslavia (Novi Sad, Kragujevac and Zaječar), rainfall fell from a mean of 511 mm for October to July in 1981--1982 to about 453 mm during the same period for the 1995--1996 season. Nevertheless, average wheat yields for new varieties in Yugoslav Commission trials during this period have shown a steady increase from 7.6 to 8.8 t ha−1. This is due to increasing the yield potential of the new varieties, even in trials giving low average yields, caused largely by drought. Thus, breeders in Yugoslavia are succeeding in improving drought resistance in new wheat varieties. However, future progress in improving drought resistance may be helped by focusing on specific traits which will help to improve either crop water use, water-use efficiency or harvest index. Thus, for example, rapid early leaf area development not only improves subsequent crop growth rates, but increases competition with weeds for water and nutrients. The rate of leaf area development is closely associated with embryo size, so selection for large embryo size should improve early growth rates. Osmotic adjustment in wheat in response to drought appears to be important for maintaining yields, and selection for high osmotic adjustment has improved drought yields. Carbon 13 discrimination (Δ) is an integral measure of plant water-use efficiency. Selecting for low Δ has also resulted in increased yield under drought conditions. Other constitutive and induced traits, such as phenology, leaf xeromorphy, excised-leaf water loss, rooting behaviour, senescence and stored assimilates are also discussed in relation to improving yields in small-grain crops. Opportunities for marker-assisted selection are also considered. Incorporating specific drought resistance traits in breeding programmes should facilitate more rapid improvement in the drought resistance of wheat and other small-grained cereals.
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  • 100
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    Genetic resources and crop evolution 46 (1999), S. 81-85 
    ISSN: 1573-5109
    Keywords: aluminum tolerance ; germplasm ; rye ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Regional rye populations and wheat cultivars/lines were screened for aluminum tolerance using the hematoxylin staining method. Portuguese regional rye populations showed better tolerance than the Polish cv. Dank. Zlote, used as tolerant tester. In the group of bread wheats, EPM 305/81, a Barbela reselection, was the most tolerant genotype with the same behaviour as the cv. BH 1146, a tolerant tester. In a study with lines selected from a local Barbela landrace, aluminum tolerance variability was detected. Some lines were as tolerant, or higher, as wheat tester. As Portuguese rye populations and the Barbela wheat landrace have grown for centuries on an acid soil region, the data supports the idea that natural biotic or abiotic stresses associated to man selection, lead to the adaptation of genotypes to specific regional conditions and, in this case, to acid soils where aluminum toxicity occurs.
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