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  • Articles  (96)
  • RFLP  (50)
  • growth  (46)
  • Springer  (96)
  • Oxford University Press
  • 2020-2023
  • 1995-1999  (96)
  • 1995  (96)
  • 1
    ISSN: 1570-7458
    Keywords: amylase inhibitor ; red kidney bean ; hard red winter wheat ; growth ; insects ; beetles ; plant resistance ; stored products ; protease inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Insect α-amylase inhibiting and/or growth inhibiting activities of proteinaceous inhibitors from red kidney bean (Phaseolus vulgaris) and hard red winter wheat (Triticum aestivum) were examined. The bean inhibitor was most effectivein vitro against α-amylases from the red flour beetle (Tribolium castaneum) and the confused flour beetle (T. confusum), followed by those from the rice weevil (Sitophilus oryzae) and yellow mealworm (Tenebrio molitor). The insect enzymes were from two- to 50-fold more susceptible than human salivary α-amylase. When the inhibitors were added at a 1% level to a wheat flour plus germ diet, the growth of red flour beetle larvae was slowed relative to that of the control group of larvae, with the bean inhibitor being more effective than the wheat inhibitor. Development of both the red flour beetle and flat grain beetle (Cryptolestes pusillus) was delayed by 1% bean inhibitor, but development of the sawtoothed grain beetle (Oryzaephilus surinamensis) and lesser grain borer (Rhyzopertha dominica) was not affected by either the bean or wheat inhibitor at the 1% level. Rice weevil adults fed a diet containing 1% bean or wheat inhibitor exhibited more mortality than weevils fed the control diet. When the wheat amylase inhibitor was combined with a cysteine protease inhibitor, E-64, and fed to red flour beetle larvae, a reduction in the growth rate and an increase in the time required for adult eclosion occurred relative to larvae fed either of the inhibitors separately. The bean inhibitor was just as effective alone as when it was combined with the protease inhibitor. These results demonstrate that plant inhibitors of insect digestive enzymes act as growth inhibitors of insects and possibly as plant defense proteins, and open the way to the use of the genes of these inhibitors for genetically improving the resistance of cereals to storage pests.
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  • 2
    ISSN: 1570-7458
    Keywords: insect endocrinology ; ecdysone ; juvenile hormone ; reproduction ; growth ; inhibin ; oostatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The first insect folliculostatic peptide was isolated from vitellogenic ovaries of the mosquitoAedes aegypti. This decapeptide directly inhibits trypsin biosynthesis in the gut, and indirectly ovarian development. The factor was named Trypsin Modulating Oostatic Factor or TMOF by its discoverers. From the fleshfly Neobellieria bullata 2 folliculostatins have been isolated, the hexapeptide Neb-TMOF and the 19-mer Neb-colloostatin. The available data suggest that at least 2 of the 3 folliculostatins originate from matrix (like) proteins present in the ovary, a hitherto unknown source of hormones. Furthermore, one of the folliculostatins (Neb-TMOF) is a potent inhibitor of ecdysone biosynthesis by larval ring glands of fleshflies. The discovery of the dipteran folliculostatins, which do not show any resemblance to inhibins of vertebrates, may significantly contribute to a better understanding of the hormonal control of growth in insects and perhaps, in other animals as well. None of the 3 folliculostatins is blocked at its N- or C-terminus. This, in combination with the pleiotropy of their effects and the narrow species specificity make such peptides prime candidates for, testing their potential in insect pest control by means of molecular biological methods.
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  • 3
    ISSN: 1432-1432
    Keywords: Escherichia coli ; RAPD ; RFLP ; Clonal theory ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of the Escherichia coli population by multilocus enzyme electrophoresis (MLEE) has established its clonal organization, but there is increasing evidence that horizontal DNA transfer occurs in E. coli. We have assessed the genetic structure of the species E. coli and determined the extent to which recombination can affect the clonal structure of bacteria. A panel of 72 E. coli strains from the ECOR collection was characterized by random amplified polymorphic DNA (RAPD) and restriction-fragment-length polymorphism (RFLP) of the ribosomal RNA gene (rrn) regions. These strains have been characterized by MLEE and are assumed to reflect the range of genotypic variation in the species as a whole. Statistical analysis, including factorial analysis of correspondence (FAC) and hierarchical classifications, established that the data obtained with the three genetic markers are mutually corroborative, thus providing compelling evidence that horizontal transfer does not disrupt the clonal organization of the population. However, there is a gradient of correlation between the different classifications which ranges from the highly clonal structure of 132 group strains causing extraintestinal infections in humans to the less-stringent structure of B1 group strains that came mainly from nonprimate mammals. This group (B1) appears to be the framework from which the remaining non-A group strains have emerged. These results indicate that RAPD analysis is well suited to intraspecies characterization of E. coli. Lastly, treating the RAPD data by FAC allowed description of subgroup-specific DNA fragments which can be used, in a strategy comparable to positional cloning, to isolate virulence genes.
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  • 4
    ISSN: 1420-9071
    Keywords: Alternating high magnetic fields ; eddy current ; magnetic flux concentration ; Caenorhabditis elegans ; growth ; reproduction ; behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Here we describe a device for testing possible influences of high magnetic fields on biological processes, by which alternating-current magnetic stimuli as high as 1.7 T can be administered. Experiments with a simple multicellular organism, the nematodeCaenorhabditis elegans, revealed that intermittent exposure to the magnetic fields modestly inhibited the animal's reproduction as well as its post-embryonic development, and caused a marked but transient derangement in its locomotory behavior. Available evidence indicates that alternating high magnetic fields can elicit both chronic and acute biological effects, but that the effects may be well tolerated or compensated for by the living organism.
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  • 5
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    Invertebrate neuroscience 1 (1995), S. 199-206 
    ISSN: 1439-1104
    Keywords: phospholipase C ; inositol phosphate ; invertebrate vision ; olfaction ; growth ; differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: abstract Phosphatidylinositol-specific phospholipase C (PLC) is a family of enzymes that occupy a pivotal role in one of the largest classes of cellular signaling pathways known. Mammalian PLC enzymes have been divided into four major classes and a variety of subclasses based on their structural characteristics and immunological differences. There have been five invertebrate PLC-encoding genes cloned thus far and these fall within three of the four major classes used in categorizing mammalian PLC. Four of these invertebrate genes have been cloned fromDrosophila melanogaster and one is fromArtemia, a brine shrimp. Structural characteristics of the invertebrate enzymes include the presence of highly conserved Box X and Box Y domains found in major types of mammalian PLC as well as novel features. Two of the invertebrate PLC genes encode multiple splice-variant subtypes which is a newly emerging level of diversity observed in mammalian enzymes. Studies of the invertebrate PLCs have contributed to the identification of the physiological functions of individual isozymes. These identified roles include cellular processes such as phototransduction, olfaction, cell growth and differentiation.
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  • 6
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    Molecular and cellular biochemistry 146 (1995), S. 63-69 
    ISSN: 1573-4919
    Keywords: rice bran oil ; growth ; plasma lipids ; fatty acids ; lipoproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effects of feeding two levels of rice bran oil (RBO) on the growth, lipid parameters, and fatty acid composition of the plasma and liver of rats (Wistar strain) were compared with those produced on animals which had been fed the same levels of peanut oil (PNO). The control animals were fed synthetic diets containing 5 and 20% peanut oil (PNO) and the experimental groups were fed similar diets, containing the same level of rice bran oil (RBO). There was no significant difference with respect to the organ weights between the control and the experimental groups. In general, groups fed 20% oil gained more weight than groups fed 5% oil. The animals which received rice bran oil in their diet had, in general, comparatively lower levels of cholesterol, triglycerides and phospholipids. On the other hand, animals receiving 20% rice bran oil in their diet, showed an increase of 20% in high density lipoproteins (HDL-C), within 18 weeks (p〈0.05), when compared to the animals fed with peanut oil. Similarly, low density lipoprotein cholesterol (LDL-C) and very low density lipoprotein cholesterol (VLDL-C) were lower in RBO-fed groups, than in the PNO-fed groups. There was, however, no significant differences in the cholesterol/phospholipid (C/P) ratio of the two groups. Analysis of plasma and of liver fatty acids indicated, in a general way, the type of fat consumed. There were no significant difference in the P/S ratio, nor any in the oleic/linoleic, oleic/stearic, palmitoleic/palmitic, oleic/palmitic, and oleic/palmitoleic ratios. Furthermore, levels of saturated (SAFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids were identical in both the groups. Thus, our results suggest that feeding a high level of rice bran oil (RBO) has no deleterious effect on the growth and blood lipid profile of rats.
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  • 7
    ISSN: 1573-2932
    Keywords: Fish populations ; perch ; roach ; growth ; reproduction ; water chemistry ; acidification ; recover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Acid-induced fish damage in small lakes in southern Finland was studied in a fish status survey of eighty lakes from 1985–1987. Later, twenty of these lakes were selected for further monitoring. A sampling of these lakes from 1988–1989 showed that the decrease in some perch (Perca fluviatilis L.) and roach (Rutilus rutilus L.) populations still continued. The results from the same lakes in 1992 showed that successful reproduction had taken place with many of the perch populations that had been close to extinction in 1985. In contrast, no signs of recovery in the roach populations were detected. The explanation for the appearance of new cohorts of perch could have been the decrease in acid deposition but the exceptional hydrological conditions of winters in the early 1990s may also have affected them. The different responses of the perch and roach populations were interpreted as a consequence of the different sensitivity of these two species to acidification. Even a slight improvement in the water quality has resulted in the appearance of strong new year-classes of perch, but not of roach. Therefore, more improvement in water quality is needed until a sensitive species like roach can reproduce again.
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  • 8
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    Water, air & soil pollution 85 (1995), S. 1461-1466 
    ISSN: 1573-2932
    Keywords: ozone ; wheat ; Triticum aestivum ; growth ; senescence ; biomass partitioning ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In closed-chamber fumigation experiments dry matter partitioning and chlorophyll fluorescence of wheat were studied, analysing the effects of ozone during different stages of plant development. Ozone causes enhanced leaf senescence, leading to a loss of green leaf area and, consequently to a decreased supply of assimilates, affecting (in increasing order of severeness) stem, ear and grain productivity because of reduced storage pools for translocation. Leaves of plants before shooting stage were most sensitive but the lack of green leaf area after ear emergence had the most pronounced effects on grain yield. Measurements of photochemical capacity showed that evidence for negative ozone effects could be found in changes of chlorophyll fluorescence parameters in leaf sections not yet showing visible ozone injury. Negative effects on photosynthesis were more distinct with increasing accumulated ozone dose, with increasing age of leaf tissue and with increasing ozone sensitivity of the cultivar. The changes in chlorophyll fluorescence are most likely to be explained by a decreased pool size of plastoquinones caused by ozone.
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  • 9
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    Molecular biology reports 22 (1995), S. 33-35 
    ISSN: 1573-4978
    Keywords: fish ; repetitive DNA ; RFLP ; satellite DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A member of satellite repetitive DNA was isolated and sequenced from a saltwater fishSillago japonica (Percoidei). This sequence consists of several oligo-dA/dT tracts and two inverted repeats which resemble each other. Dot blot hybridization analysis using a satellite DNA clone pSJ2 among the species in the suborder Percoidei revealed that the pSJ2 sequence was amplified at least after the family Sillaginidae had been derived.
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  • 10
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    Theoretical and applied genetics 90 (1995), S. 1063-1067 
    ISSN: 1432-2242
    Keywords: T. monococcum ssp. monococcum ; T. monococcum ssp. boeoticum ; T. urartu ; RFLP ; Diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The A genome of the Triticeae is carried by three diploid species and subspecies of the genus Triticum: T. monococcum ssp. monococcum, T. monococcum ssp. boeoticum, and T. urartu, the A-genome donor of bread wheat. These species carry many genes of agronomic interest, including disease resistances, and may also be used for the genetic mapping of the A genome. The aim of this study was to evaluate the variability present in a sample of 25 accessions representative of this group using RFLP markers. Twenty probes, consisting of genomic DNA or cDNA from wheat, were used in combination with four restriction enzymes. A high level of polymorphism was found, especially at the interspecific level. Selecting the most informative enzymes appeared to be of great importance in order to obtain a stable structure for the diversity observed with only 20 probes. The results are largely consistent with taxonomy and data relating to geographical origins. The probes were also tested on 14 wheat cutivars. A good correlation coefficient was found for their informative values on wheat cultivars and diploid lines. Whether the group of species studied here would be useful for genetic mapping remains to be determined. Nevertheless, RFLP markers will be useful to follow genes that can possibly be introgressed from these species into cultivated wheat.
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  • 11
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    Theoretical and applied genetics 90 (1995), S. 1198-1203 
    ISSN: 1432-2242
    Keywords: Diplospory ; RFLP ; Bulk-segregant analysis ; Genome similarity ; Intergeneric hybrids ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyploid plants in the genus Tripsacum, a wild relative of maize, reproduce through gametophytic apomixis of the diplosporous type, an asexual mode of reproduction through seed. Moving gene(s) responsible for the apomictic trait into crop plants would open new areas in plant breeding and agriculture. Efforts to transfer apomixis from Tripsacum into maize at CIMMYT resulted in numerou intergeneric F1 hybrids obtained from various Tripsacum species. A bulk-segregant analysis was carried out to identify molecular markers linked to diplospory in T. dactyloides. This was possible because of numerous genome similarities among related species in the Andropogoneae. On the basis of maize RFLP probes, three restriction fragments co-segregating with diplospory were identified in one maize-Tripsacum dactyloides F1 population that segregated 1∶1 for the mode of reproduction. The markers were also found to be linked in the maize RFLP map, on the distal end of the long arm of chromosome 6. These results support a simple inheritance of diplospory in Tripsacum. Manipulation of the mode of reproduction in maize-Tripsacum backcross generations, and implications for the transfer of apomixis into maize, are discussed.
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  • 12
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    Theoretical and applied genetics 91 (1995), S. 448-456 
    ISSN: 1432-2242
    Keywords: QTLs ; RFLP ; Pearl millet ; Downy mildew resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Quantitative trait loci (QTLs) for resistance to pathogen populations of Scelerospora graminicola from India, Nigeria, Niger and Senegal were mapped using a resistant x susceptible pearl millet cross. An RFLP map constructed using F2 plants was used to map QTLs for traits scored on F4 families. QTL analysis was carried out using the interval mapping programme Mapmaker/QTL. Independent inheritance of resistance to pathogen populations from India, Senegal, and populations from Niger and Nigeria was shown. These results demonstrate the existence of differing virulences in the pathogen populations from within Africa and between Africa and India. QTLs of large effect, contributing towards a large porportion of the variation in resistance, were consistently detected in repeated screens. QTLs of smaller and more variable effect were also detected. There was no QTLs that were effective against all four pathogen populations, demonstrating that pathotype-specific resistance is a major mechanism of downy mildew resistance in this cross. For all but one of the QTLs, resistance was inherited from the resistant parent and the inheritance of resistance tended to be the result of dominance or over-dominance. The implications of this research for pearl millet breeding are discussed.
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  • 13
    ISSN: 1432-2242
    Keywords: Brassica rapa ; RFLP ; RAPD ; QTL ; Palmitic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract F2 progeny (105 individuals) from the cross Jo4002 x Sv3402 were used to identify DNA markers associated with palmitic-acid content in spring turnip rape (Brassica rapa ssp. oleifera). QTL mapping and ANOVA analysis of 140 markers exposed one linkage group with a locus controlling palmitic-acid content (LOD score 27), and one RAPD (random amplified polymorphic DNA) marker, OPB-11a, closely linked (1.4 cM) to this locus. Palmitic-acid content in the 62 F2 plants with the visible allele of marker OPB-11a was 8.45 ±3.15%, while that in the 24 plants without it was 4.59 ±0.97%. As oleic-acid concentration is affected by a locus on the same linkage group as the palmitic-acid locus, this locus probably controls the chain elongation from palmitic acid to oleic acid (through stearic acid). Marker OPB-11a may be used in future breeding programs of spring turnip rape to simplify and hasten the selection for palmitic-acid content.
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  • 14
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    Theoretical and applied genetics 91 (1995), S. 505-509 
    ISSN: 1432-2242
    Keywords: Chloroplast DNA ; Mitochondrial DNA ; rDNA ; RFLP ; Witloof chicory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment length polymorphisms of cytoplasmic DNAs and nuclear rDNA were analyzed in several Cichorium intybus genotypes, comprising four white inbred lines, eight red witloof experimental lines, and a number of F1 hybrids derived from two white parents. Chloroplast and mitochondrial restriction patterns led to the distinction between two different cytoplasms, called I and II. Southern hybridization using a nuclear rDNA probe revealed that all the lines possessed two types of rDNA repeat units. The shortest unit was 10 kb and was common to all lines. The largest rDNA repeat unit was 10.5 kb in lines I and 10.4 kb in lines II. In addition, a sequence heterogeneity between the 10.5 and 10.4-kb rDNA repeat units was revealed by Sac I digestion. A 10-kb rDNA unit was successively cloned, mapped, and used as a probe to check the genetic purity of F1 hybrid seeds between line I and II white parents. We found a 30% average percentage of impurities, originating both from selfing and full-sib crossing, in different open-pollinated hybrid samples.
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  • 15
    ISSN: 1432-2242
    Keywords: Glycine max ; Heterodera glycines ; RFLP ; Genetic mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Resistance to the soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is difficult to evaluate in soybean [Glycine max (L.) Merr.] breeding. PI 437.654 has resistance to more SCN race isolates than any other known soybean. We screened 298 F6∶7 recombinant-inbred lines from a cross between PI 437.654 and ‘BSR101’ for SCN race-3 resistance, genetically mapped 355 RFLP markers and the I locus, and tested these markers for association with resistance loci. The Rhg 4 resistance locus was within 1 cM of the I locus on linkage group A. Two additional QTLs associated with SCN resistance were located within 3cM of markers on groups G and M. These two loci were not independent because 91 of 96 lines that had a resistant-parent marker type on group G also had a resistant-parent marker type on group M. Rhg 4 and the QTL on G showed a significant interaction by together providing complete resistance to SCN race-3. Individually, the QTL on G had greater effect on resistance than did Rhg 4, but neither locus alone provided a degree of resistance much different from the susceptible parent. The nearest markers to the mapped QTLs on groups A and G had allele frequencies from the resistant parent indicating 52 resistant lines in this population, a number not significantly different from the 55 resistant lines found. Therefore, no QTLs from PI 437.654 other than those mapped here are expected to be required for resistance to SCN race-3. All 50 lines that had the PI 437.654 marker type at the nearest marker to each of the QTLs on groups A and G were resistant to SCN race-3. We believe markers near to these QTLs can be used effectively to select for SCN race-3 resistance, thereby improving the ability to breed SCN-resistant soybean varieties.
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  • 16
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    Theoretical and applied genetics 91 (1995), S. 681-690 
    ISSN: 1432-2242
    Keywords: RFLP ; Barley ; Hordeum vulgare ssp. spontaneum ; Gramineae ; Comparative mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several gene linkage maps have been produced for cultivated barley. We have produced a new linkage map for barley, based on a cross between Hordeum vulgare subsp. spontaneum and Hordeum vulgare subsp. vulgare (Hvs x Hvv), having a higher level of polymorphism than most of the previous barley crosses used for RFLP mapping. Of 133 markers mapped in the Hvs x Hvv F2 population, 69 were previously mapped on other barley maps, and 26 were mapped in rice, maize, or wheat. Two known gene clones were mapped as well as two morphological markers. The distributions of previously mapped markers were compared with their respective barley maps to align the different maps into one consensus map. The distributions of common markers among barley, wheat, rice and maize were also compared, indicating colinear linkage groups among these species.
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  • 17
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    Theoretical and applied genetics 91 (1995), S. 795-801 
    ISSN: 1432-2242
    Keywords: Salt stress ; Water deficient ; Heat shock ; Mapping ; Triticeae ; RFLP ; Linkage map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Linkage relationships among genes responding to water-deficit, salt stress, and heat shock were investigated in diploid wheat, Triticum monococcum L. The position of these gene loci relative to closely linked markers and the centromeres is reported. It is proposed to continue to use the present T. monococcum mapping population and the genetic maps based thereon as a framework for future determination of relationships among other genes related to environmental stress in the tribe Triticeae.
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  • 18
    ISSN: 1432-2242
    Keywords: Heterozygosity ; Oryza sativa ; Heterosis ; RFLP ; Recombinant inbred lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Forty-seven recombinant inbred (RI) lines derived from a cross between two indica rices, cv ‘Phalguna’ and the Assam land race ARC 6650, were subjected to restriction fragment length polymorphism (RFLP) analysis using cloned probes defining 150 single-copy loci uniformly dispersed on the 12 chromosomes of rice. Of the probes tested, 47 detected polymorphism between the parents. Heterozygosity was calculated for each line and for each of the polymorphic loci. Average heterozygosity per line was 9.6% but was excessive (〉20%) in the 5 lines that seemed to have undergone outcrossing immediately prior to harvest. Average heterozygosity detected by each probe across the 47 RI lines was 9.7%. The majority of probes revealed the low level of heterozygosity (〈8%) expected for F5-F6 lines in a species showing about 5% outbreeding. On the other hand, 7 probes exhibited heterozygosity in excess of 15%, while with a eighth probe (RG2 from chromosome 11) heterozygosity varied according to the restriction enzyme employed, ranging from 2% with SaII to 72% with EcoRV. The presence of 34 recombination sites in a segment of the genome as short as 24 kb indicates a strong selection for recombination between two neighbouring loci, one required as homozygous for the ‘Phalguna’ allele, and the other heterozygous. Since selection was principally for yield advantage over that of the high-yielding parent, ‘Phalguna’, one or both of these loci may be important for heterosis in this cross. The results also indicate that heterozygosity as measured by RFLP can depend on the particular restriction endonuclease employed.
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  • 19
    ISSN: 1432-2242
    Keywords: Leaf rust ; RFLP ; RAPD ; Wheat ; Agropyron elongatum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this study was to identify molecular markers linked to the wheat leaf rust resistance gene Lr24 derived from Agropyron elongatum (3DL/3Ag translocation). Two near isogenic lines (NILs), ‘Arina’ and Lr24/7 * “Arina”, were screened for polymorphism at the DNA level with 115 RFLP probes. Twenty-one of these probes map to the homoeologous group 3. In addition, 360 RAPD primers were tested on the NILs. Six RFLP probes showed polymorphism between the NILs, and 11 RAPD primers detected one additional band in the resistant NIL. The genetic linkage of the polymorphic markers with Lr24 was tested on a segregating F2 population (150 plants) derived from a cross between the leaf rust resistant Lr24/7 * “Arina” and the susceptible spelt (Triticum spelta) variety ‘Oberkulmer’. All 6 RFLP markers were completely linked to Lr24: one was inherited as a codominant marker (PSR1205), one was in coupling phase (PSR1203) and 4 were in repulsion phase (PSR388, PSR904, PSR931, PSR1067) with Lr24. The localization of these probes on chromosome 3D was confirmed by nulli-tetrasomic analysis. Distorted genotypic segregation was found for the Codominant RFLP marker PSR1205. This distortion can be explained by the occurrence of hemizygous plants. One of the 11 RAPD markers (OPJ-09) also showed complete linkage to theLr24 resistance gene. The polymorphic RAPD fragment was cloned and sequenced. Specific primers were synthesized, and they produced an amplification product only in the resistant plants. This specific marker allows a reliable and rapid screening of a large number of genotypes in practical breeding. Analysis of 6 additional lines containing Lr24 revealed that 3 lines have a smaller chromosomal segment of A. elongatum than lines derived from ‘Agent’, a commonly used gene donor for the Lr24 resistance gene.
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  • 20
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    Theoretical and applied genetics 91 (1995), S. 869-875 
    ISSN: 1432-2242
    Keywords: Genetic map ; Linkage ; Eucalypts ; RFLP ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An integrated genetic linkage map for E. nitens was constructed in an outbred three-generation pedigree. Analysis of 210 RFLP, 125 RAPD and 4 isozyme loci resulted in 330 markers linked in 12 linkage groups covering 1462 cM (n=11 in eucalypts). The 12th linkage group is comprised of only 5 markers and will probably coalesce with another linkage group when further linked loci are located. Co-dominant RFLP loci segregating in both parents were used to integrate linkages identified in the male and female parents. Differences in recombination frequencies in the two parents were observed for a number of pairs of loci, and duplication of sequences was identified both within and between linkage groups. The markers were distributed randomly across the genome except for the RFLPs in linkage group 10 and for some loci showing segregation distortion, which were clustered into three regions of the map. The use of a large number of co-dominant RFLP loci in this map enables it to be used in other pedigrees of E. nitens and forms a basis for the detection and location of QTL in E. nitens and other eucalypt species.
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  • 21
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    Theoretical and applied genetics 91 (1995), S. 1210-1213 
    ISSN: 1432-2242
    Keywords: Stylosanthes ; DNA isolation ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A DNA isolation method suitable for genomic library construction and RFLP analyses of the forage legume Stylosanthes was developed. Probes isolated using this method were used to investigate the feasibility of constructing RFLP-based genetic maps in this genus. Two hundred and seventy-one PstI genomic DNA and 134 cDNA clones were analysed against four Stylosanthes accessions, including two tetraploids and two diploids, with the use of two restriction enzymes, DraI and HindIII. The proportion of clones which detected single-copy sequences from the PstI genomic library was higher than that from the cDNA library, but the percentage of clones which detected low-copy sequences was doubled in the latter. There was no significant difference in the level of RFLPs detected by gDNA and cDNA probes, although the level of polymorphism was lower in the diploids. A large proportion of RFLPs seemed to have resulted from mutation/base substitution events, and this was especially the case in diploids.
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  • 22
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    Theoretical and applied genetics 90 (1995), S. 331-334 
    ISSN: 1432-2242
    Keywords: Interspecific poplar hybrids ; Non-parental chloroplast DNA fragments ; Novel organelle DNA ; RFLP ; Chloroplast DNA recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rare phenomenon of the occurrence of novel non-parental chloroplast DNA (cpDNA) variants in natural sexual interspecific hybrids between Populus deltoides var deltoides and P. nigra, P. x canadensis is described. Restriction fragment variation of cpDNA in 17 P. x canadensis cultivars was examined and compared with that of representative samples of P. deltoides and P. nigra using 83 combinations of 16 restriction enzymes and six Petunia hybrida cpDNA probes. Twelve cultivars had one to five novel non-parental cpDNA fragments in the chloroplast genome region homologous to the 9.0-kb PstI cpDNA fragment of Petunia from the large single-copy region.
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  • 23
    ISSN: 1432-2242
    Keywords: Molecular markers ; RFLP ; PCR ; SAPs ; DNA sequence ; Marker-assisted selection
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    Notes: Abstract The genomic clone RG64, which is tightly linked to the blast resistance gene Pi-2(t) in rice, provides means to perform marker-aided selection in a rice breeding program. The objective of this study was to investigate the possibility of generating a polymerase chain reaction (PCR)-based polymorphic marker that can distinguish the blast resistant gene, Pi-2(t), and susceptible genotypes within cultivated rice. RG64 was sequenced, and the sequence data was used to design pairs of specific primers for (PCR) amplification of genomic DNA from rice varieties differing in their blast disease responsiveness. The amplified products, known as sequenced-tagged-sites (STSs), were not polymorphic between the three varieties examined. However, cleavage of the amplified products with the restriction enzyme HaeIII generated a polymorphic fragment, known as specific amplicon polymorphism (SAP), between the resistant and the susceptible genotypes. To examine the power of the identified SAP marker in predicting the genotype of the Pi-2 (t) locus, we determined the genotypes of the F2 individuals at this locus by performing progeny testing for the disease response in the F3 generation. The results indicated an accuracy of more than 95% in identifying the resistant plants, which was similar to that using RG64 as the hybridization probe. The identification of the resistant homozygous plants increased to 100% when the markers flanking the genes were considered simultaneously. These results demonstrate the utility of SAP markers as simple and yet reliable landmarks for use in marker-assisted selection and breeding within cultivated rice.
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  • 24
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    Theoretical and applied genetics 91 (1995), S. 150-159 
    ISSN: 1432-2242
    Keywords: RFLP ; Mitochondrial DNA diversity ; Natural populations ; Cytoplasmic male sterility ; Daucus carota L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondrial variability was investigated in natural populations of wild carrot (Daucus carota ssp carota) in different regions: South of France, Greece, and various sites in the Mediterranean Basin and Asia. Total DNA was digested with two restriction endonucleases (EcoRV and HindIII) and probed with three mitochondrial DMA-specific genes (coxI, atp6, and coxII). Twenty-five different mitochondrial types were found in 80 analyzed individuals. Thirteen mitotypes were found among the 7 French populations studied. On average, 4.4 different mitotypes were observed per population, and these mitotypes were well-distributed among the populations. All of the mitochondrial types were specific to a single region. However, the proportion of shared restriction fragments between 2 mitotypes from different regions was not particularly lower than that which occurred among mitotypes from a single region. On the basis of the sexual phenotype [male-sterile (MS) or hermaphrodite] of the plants studied in situ and that of their progeny, 2 mitotypes were found to be highly associated with male sterility. Eighty percent of the plants bearing these mitotypes were MS in situ, and all of these plants produced more than 30% MS plants in their progeny. This association with male sterility was consistent in several populations, suggesting an association with a cytoplasmic male-sterility system. Moreover, these two mitotypes had very similar mitochondrial DNA restriction patterns and were well-differentiated from the other mitotypes observed in wild plants and also from those observed in the two CMS types already known in the cultivated carrot. This suggests that they correspond to a third cytoplasmic sterility.
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  • 25
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    Theoretical and applied genetics 90 (1995), S. 1-10 
    ISSN: 1432-2242
    Keywords: Brassica rapa ; Brassica campestris ; Morphological variation ; Quantitative trait loci ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Construction of a detailed RFLP linkage map of B. rapa (syn. campestris) made it possible, for the first time, to study individual genes controlling quantitative traits in this species. Ninety-five F2 individuals from a cross of Chinese cabbage cv ‘Michihili’ by Spring broccoli were analyzed for segregation at 220 RFLP loci and for variation in leaf, stem, and flowering characteristics. The number, location, and magnitude of genes underlying 28 traits were determined by using an interval mapping method. Zero to five putative quantitative trait loci (QTL) were detected for each of the traits examined. There were unequal gene effects on the expression of many traits, and the inheritance patterns of traits ranged from those controlled by a single major gene plus minor genes to those controlled by polygenes with small and similar effects. The effect of marker locus density on detection of QTL was analyzed, and the results showed that the number of QTL detected did not change when the number of marker loci used for QTL mapping was decreased from 220 to 126; however, a further reduction from 126 to 56 caused more than 15% loss of the total QTL detected. The detection of putative minor QTL by removing the masking effects of major QTL was explored.
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    Theoretical and applied genetics 90 (1995), S. 787-796 
    ISSN: 1432-2242
    Keywords: Sorghum ; RFLP ; Phylogeny Gene flow
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    Topics: Biology
    Notes: Abstract Sixty-two single-copy sorghum DNA clones were used to compare restriction fragment patterns of 53 sorghum accessions from Africa, Asia and the United States. Included were accessions from five morphological races of the cultivated subspecies bicolor, and four races of the wild subspecies verticilliflorum. From two to twelve alleles were detected with each probe. There was greater nuclear diversity in the wild subspecies (255 alleles in ten accessions) than in the domestic accessions (236 alleles in 37 accessions). Overall, 204 of the 340 alleles (60%) that were detected occurred in both subspecies. Phylogenetic analysis using parsimony separated the subspecies into separate clusters, with one group of intermediate accessions. Though exceptions were common, especially for the race bicolor, accessions classified as the same morphological race tended to group together on the basis of RFLP similarities. Selection for traits such as forage quality may have led to accessions genetically more similar to other races being classified as bicolors, which have a loose, small-grained panicle similar to wild races. Population statistics, calculated using four nuclear and four cytoplasmic probes that detect two alleles each, revealed a low but significant amount of heterozygosity, and showed little differentiation in alleles in the wild and cultivated subspecies. Outcrossing with foreign pollen appears to have been more important than migration via seed dispersal as a mechanism for gene flow between the wild and domestic accessions included in this study.
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  • 27
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    Theoretical and applied genetics 90 (1995), S. 1079-1086 
    ISSN: 1432-2242
    Keywords: RFLP ; Helianthus annuus ; Linkage map ; Consensus map ; CDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper provides the first description of a consensus map of the cultivated sunflower genome (Helianthus annuus L., n=17 chromosomes), based on RFLP. A total of 180 probe-enzyme combinations were mapped on at least one of five segregating progenies (three F2 and two BC1 populations), revealing 237 loci that did not show any distortion of segregation. The consensus linkage map obtained with these loci covers 1150 cM and consists of 16 linkage groups of more than 20 cM, 7 groups of less than 20 cM and 18 unlinked loci. The mean distance between loci is 7 cM, but in some regions intervals of 20 cM remain. Genotypic and gametic segregation distortions affect about 7% of loci. It was found that 25% of the probes mapped using several different restriction enzymes or that on different progenies they revealed 2 or more loci.
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  • 28
    ISSN: 1432-2242
    Keywords: Saccharum ; Intergeneric hybrids ; Isozymes ; RFLP ; STS-PCR ; In situ hybridization ; rDNA
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    Notes: Abstract Molecular markers were used to characterise sugarcane intergeneric hybrids between S. officinarum and E. arundinaceus. Very simple diagnostic tools for hybrid identification among the progeny were derived from isozyme electrophoresis and a sequence-tagged PCR. Two enzyme systems (GOT and MDH B) and PCR amplification revealing spacer-size variation in the 5s-rDNA cluster were found most convenient. Specific characterisation of the two genomic components was possible using RFLP and in situ hybridisation. The strong molecular differentiation between S. officinarum and E. arundinaceus allows the identification of numerous Erianthus-specific RFLP bands in the hybrids. Genomic DNA in situ hybridisation allows for the differentiation of the chromosomes contributed by S. officinarum and E. arundinaceus in chromosome preparations of the hybrids. In situ hybridisation with the 18s-5.8s-25s rDNA probe highlights the basic chromosome numbers in the two parental species. The potential of these techniques to monitor the Erianthus genome during the introgression process is discussed.
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  • 29
    ISSN: 1432-2242
    Keywords: RFLP ; QTL ; Rice ; Heading date ; Plant height ; Pleiotropic effects
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    Notes: Abstract ‘Lemont’ and ‘Teqing’ are both semidwarf rice varieties that differ in heading date by only 6 days. However, when ‘Lemont’ and ‘Teqing’ are crossed there is transgressive segregation for both heading date (HD) and plant height (PH). By testing 2418 F4 lines with 113 well-distributed RFLP markers, we identified and mapped chromosomal regions that were largely responsible for this transgressive segregation. QHd3a, a QTL from ‘Lemont’ that gives 8 days earlier heading, was identified on chromosome 3 approximately 3 cM from the marker RG348. Another QTL with a large effect, QHd8a, which gives 7 days earlier heading, was identified on chromosome 8 of ‘Teqing’ between RG20 and RG1034. Along with a QTL, QHd9a with a phenotypic effect of 3.5 days, these genomic regions collectively explain 76.5% of the observed phenotypic variance in heading date. Four QTLs which altered plant height from 4 to 7 cm were also mapped; these collectively explain 48.8% of the observed phenotypic variation in plant height. None of the QTLs for plant height mapped to chromosome 1, the location of the semidwarf gene sd-1. All three of the HD loci mapped to approximately the same genomic locations as PH QTLs, and in all cases, there was a reduction in height of approximately 1 cm for every day of earlier heading. The correspondence between the HD and some of the PH loci suggests that genes at these chromosome locations may have pleiotropic effects on both HD and PH. The observed heterosis in the F1 plants for HD can be largely explained by the dominance for earliness of the identified HD loci and distribution of earlier heading alleles in the parents. However, overdominance observed at one of the PH QTL may, at least in part, be responsible for the observed heterosis in PH.
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    Theoretical and applied genetics 91 (1995), S. 780-782 
    ISSN: 1432-2242
    Keywords: Physical mapping ; RFLP ; Cereals ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytologically based physical maps for the group 3 chromosomes of wheat were constructed by mapping 25 Triticum aestivum deletion lines with 29 T. tauschii and T. aestivum RFLP probes. The deletion lines divide chromosomes 3A, 3B, and 3D into 31 discrete intervals, of which 18 were tagged by marker loci. The comparison of the consensus physical map with a consensus RFLP linkage map of the group 3 chromosomes of wheat revealed a fairly even distribution of marker loci on the long arm, and higher recombination in the distal region.
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    Theoretical and applied genetics 91 (1995), S. 720-726 
    ISSN: 1432-2242
    Keywords: Secale cereale ; RFLP ; Cytogenetic mapping ; C-band ; In situ hybridization
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    Notes: Abstract A genetic map of rye, Secale cereale L., chromosome 1R covering 247 cM was constructed utilizing 27 RFLP and four C-band markers, including terminal C-bands. Genetic mapping of C-bands and the centromere, and in situ hybridization of three RFLP clones, allowed for the integration of the genetic and cytological maps. Eight contact points between the genetic and cytological maps revealed variation in the recombination distance to cytological distance ratio ranging between 0.25 and 1.95, a 7.8-fold difference. Recombination was found to be highest in the satellite region of 1RS and lowest in the most distal region of 1RL.
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  • 32
    ISSN: 1432-2242
    Keywords: Rice TGMS gene ; RAPD ; RFLP ; Molecular markers ; Gene tagging
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    Notes: Abstract The thermo-sensititve genic male-sterile (TGMS) gene in rice can alter fertility in response to temperature and is useful in the two-line system of hybrid rice production. However, little is known about the TGMS gene at the molecular level. The objective of this study was to identify molecular markers tightly linked with the TGMS gene and to map the gene onto a specific rice chromosome. Bulked segregant analysis of an F2 population from 5460s (a TGMS mutant line) x ‘Hong Wan 52’ was used to identify RAPD markers linked to the rice TGMS gene. Four hundred RAPD primers were screened for polymorphisms between the parents and between two bulks representing fertile and sterile plants; of these, 4 primers produced polymorphic products. Most of the polymorphic fragments contained repetitive sequences. Only one singlecopy sequence fragment was found, a 1.2-kb fragment amplified by primer OPB-19 and subsequently named TGMS1.2. TGMS1.2 was mapped on chromosome 8 with a RIL population and confirmed by remapping with a DHL population. Segregation analysis using TGMS1.2 as a probe indicated that TGMS1.2 both consegregated and was lined with the TGMS gene in this population. It is located about 6.7 cM from the TGMS gene. As TGMS1.2 is linked to the TGMS gene, the TGMS gene must be located on chromosome 8.
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    Theoretical and applied genetics 90 (1995), S. 17-26 
    ISSN: 1432-2242
    Keywords: Tomato (Lycopersicon esculentum) ; Genetic map ; RFLP ; Integrated map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The classical map of the short arm of chromosome 1 of tomato (Lycopersicon esculentum) has been shown to contain inaccuracies while the RFLP map of this region is known to be generally accurate. Molecular analysis of populations derived from crosses between L. esculentum lines carrying chromosome 1 classical markers and L. pennellii has enabled us to produce an integrated classical and RFLP marker map of this region. New data concerning the linkage relationships between classical markers have also been combined with previous data to produce a new classical map of the short arm of chromosome 1. The orders of the classical markers on these two new maps are in almost complete agreement and are very different to that shown on the previous classical map.
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    Theoretical and applied genetics 90 (1995), S. 173-181 
    ISSN: 1432-2242
    Keywords: RFLP ; Gliadin alleles ; Organization of Gli-1 loci ; Gli-2-type sequences on chromosome 1R Common wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A correspondence between RFLP patterns and gliadin alleles at the Gli-1 and Gli-2 loci was established in a set of 70 common wheat (T.aestivum L.) cultivars using γ-gliadin (K32) and α-gliadin (pTU1) specific probes. All Gli-B1 and Gli-D1 alleles which differed in encoded γ-gliadins showed definite RFLP patterns after hybridization with the K32 probe. Two groups of Gli-B1 alleles, Gli-B1b-like and Gli-B1e-like, were identified, and these could originate from distinct genotypes of the presumptive donor of the B-genome. Intralocus recombination and/or gene conversion as well as small deletions, gene silencing and gene amplification were assumed to be responsible for the origin of new gliadin alleles. Silent γ-gliadin sequences were shown to exist in all of the genotypes studied. K32 also differentiated Gli-A1a from all other Gli-A1 alleles as well as the Gli-B11 allele in cultivars carrying the 1B/1R (wheat/rye) translocation. PTU1 was shown to recognize several Gli-A2 alleles, but not the Gli-B2 or Gli-D2 alleles. Moreover, this probe hybridized to chromosome 1R sequences suggesting the existence of rye gene(s), probably silent, for α-gliadin-like proteins on chromosome 1R.
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  • 35
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    Theoretical and applied genetics 90 (1995), S. 341-346 
    ISSN: 1432-2242
    Keywords: Disease resistance ; High resolution genetic map ; Recombination ; Ribosomal DNA ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mdm1 locus on the short arm of chromosome six confers resistance in maize to five strains of the maize dwarf mosaic virus (MDMV), an aphid transmitted potyvirus. The location of mdm1 in relation to RFLP and morphological loci on the short arm of chromosome six was determined using BC1 and F2 mapping populations. The following map order and distance in cM was obtained from the F2 population; jc1270-2.5-npi245-1.6-umc85/po1-0.5-mdm1/nor-0.5-bnl6.29A-0.5-npi235-0.8-npi101A-4.3-numc59. No recombination between mdm1 and the nucleolus organizer region (nor) was detected, as determined using a probe from the intergenic spacer region of the rDNA repeat. In order to resolve the relationship between mdm1 and the nor, and to recover recombinants around mdm1, a highresolution map within the polymitotic1 (po1) yellow kernel1 (y1) interval was generated using [po1 y1 tester (po1 mdm1 y1) x Pa405 (Po1 Mdm1 Y1)] F2 plants. The recessive po1 allele imparts a male-sterile phenotype when homozygous and since po1 and y1 are closely linked, the majority of fertile plants from white endosperm (y1/y1) F2 kernels will arise though a recombination event between the Pa405 Po1 allele and the y1 allele of the po1 y1 tester. Plants from 7,650 white (y1/y1) F2 kernels were examined (15,300 chromosomes) and a total of 626 F2∶3 recombinant families was recovered. Analysis of these recombinants revealed that mdm1 cosegregates with the nor. This lack of recombination between mdm1 and the nor suggests that: either (1) mdm1 is located in the region flanking the nor and recombination is suppressed within that region, or (2) mdm1 is located within the nor.
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    Theoretical and applied genetics 90 (1995), S. 129-134 
    ISSN: 1432-2242
    Keywords: Wheat ; RFLP ; Triticum dicoccoides ; T. araraticum ; Genetic divergence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Intra- and inter-specific variations in the nuclear DNA of Triticum dicoccoides Körn. (2n = 28, genome constitution AABB) and T. araraticum Jakubz. (2n = 28, AAGG), wild species, respectively, of the Emmer and Timopheevi group, were studied by restriction fragment length polymorphism (RFLP) analysis. Total DNAs of 32 T. dicoccoides and 24 T. araraticum accessions, collected from throughout the distribution areas of these species, were treated with two 6-bp cutters and hybridized with 30 nuclear DNA clones as probes to detect RFLPs. A total of 167 hybrid bands were observed per accession. All the enzyme-probe combinations showed RFLPs between accessions. The average genetic distance between the T. dicoccoides accessions was 0.0135 ± 0.0031 and that between the T. araraticum accessions 0.0036 ± 0.0015, indicative of about a four-fold intraspecific variation in T. dicoccoides as compared to T. araraticum in terms of genetic distance. No significant genetic differentiation was found for the geographical populations of these species, the genetic distance between the two species being 0.0482 ± 0.0022. The interspecific divergence corrected for intraspecific divergence was 0.0395, about three times that for T. dicoccoides and 11 times that for T. araraticum. The results show that in the wild state the Emmer and Timopheevi groups are clearly differentiated and that T. dicoccoides has much greater variation than T. araraticum, suggesting a relatively recent origin for the latter and therefore a diphyletic origin for these species.
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    Theoretical and applied genetics 90 (1995), S. 189-193 
    ISSN: 1432-2242
    Keywords: Sugar beet ; Beta vulgaris ; RFLP ; RAPD
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    Topics: Biology
    Notes: Abstract An updated map of sugar beet (Beta vulgaris L. ssp. vulgaris var ‘altissima Doell’) is presented. In this genetic map we have combined 248 RFLP and 50 RAPD loci. Including the loci for rhizomania resistance Rr1, hypocotyl colour R and the locus controlling the monogerm character M, 301 loci have now been mapped to the nine linkage groups covering 815 cM. In addition, the karyotype of some of the Beta vulgaris chromosomes has been correlated with existing RFLP and RAPD linkage maps.
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    Theoretical and applied genetics 90 (1995), S. 451-456 
    ISSN: 1432-2242
    Keywords: Lycopersicon esculentum ; Lycopersicon peruvianum ; RAPD ; RFLP ; Tomato spotted wiltvirus (TSWV)
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    Notes: Abstract The Sw-5 locus confers dominant resistance to tomato spotted wilt virus (TSWV). To map the location and facilitate the identification of markers linked to Sw-5 we developed a pair of near-isogenic lines (NILs) and an F2 Lycopersicon esculentum x L. pennellii population segregating for resistance to TSWV. DNA from the NILs was analyzed using 748 random 10-mer oligonucleotides to discern linked molecular markers using a random amplified polymorphic DNA (RAPD) approach. One random primer (GAGCACGGGA) was found to produce a RAPD band of about 2200 bp that demonstrates linkage to Sw-5. Data from co-segregation of resistance and restriction fragment length polymorphisms (RFLPs) in a F2 interspecific population position Sw-5 between the markers CT71 and CT220 near the telomere of the long arm of chromosome 9.
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    Theoretical and applied genetics 90 (1995), S. 643-649 
    ISSN: 1432-2242
    Keywords: Oil palm ; Elaeis guineensis Elaeis oleifera ; RFLP ; Genetic fingerprinting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A variety of DNA probes was used to screen a diverse set of oil palm accessions in order to identify markers with a utility in genotype discrimination. This survey included samples of the commercial oil palm native to Africa (Elaeis guineensis Jacq.), the closely-related South American species [E.oleifera (HBK) Cortes] and inter-specific hybrids of the two. Of 106 major chloroplast bands none showed differences between E. guineensis and E. Oleifera. Mitochondrial and ribosomal probes were more informative inter-specifically (the former allowing identification of the maternal inheritance of mitochondria) and may be useful in hybrid breeding programmes; however, they were unable to identify polymorphism within E. guineensis. In contrast, low-copy nuclear genomic clones were able to identify intra-specific variation, though in most cases they revealed a relatively small number of allelic variants. One DNA probe showed a much larger number of band variants, revealing ten patterns amongst 13 E. guineensis accessions, and should prove useful in genetic fingerprinting and evaluation of oil-palm germplasm collections.
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  • 40
    ISSN: 1432-2242
    Keywords: RFLP ; RAPD ; Linkage map ; Sugi (Cryptomeria japonica) ; Three-generation pedigree
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Abstract A linkage map for sugi was constructed on the basis of restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and isozyme loci using a three-generation pedigree prepared for genetic analysis of heartwood color. A total of 128 RFLP (123 cDNA and 5 genomic probes), 33 RAPD, 2 isozyme, and 1 morphological (dwarf) loci segregated in 73 progeny. Of the 164 segregating loci, 145 loci were distributed in 20 linkage groups. Of these loci, 91 with confirmed map positions were assigned to 13 linkage groups, covering a total of 887.3 cM. A clustering of markers with distorted segregation was observed in 6 linkage groups. In the four clusters, distortions with a reduction in the number of homozygotes from one parent only were found.
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    Theoretical and applied genetics 91 (1995), S. 62-67 
    ISSN: 1432-2242
    Keywords: Brassica napus ; Resynthesized rapeseed ; Genetic distance ; Isozymes ; Allozymes ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It has frequently been suggested to use the resynthesis of rapeseed (Brassica napus) from B. campestris and B. oleracea to broaden its genetic base. The objective of the present study is twofold: (1) to compare the genetic variation within resynthesized rapeseed with a world-wide collection of oilseed rape cultivars, and (2) to compare genetic distances estimated from RFLP markers with distances estimated from a relatively small number of allozyme markers. We investigated 17 resynthesized lines and 24 rapeseed cultivars. Genetic distances were estimated either based on the electrophoresis of seven allozymes, with a total of 38 different bands, or based on RFLP data of 51 probe/enzyme combinations, with a total of 355 different bands. The results of allozyme and RFLP analyses agreed reasonably well. Genetic distances, estimated from two independent sets of RFLP data with 25 and 26 probe/enzyme combinations respectively, were highly correlated; hence about 50 RFLP markers are sufficient to characterize rapeseed material with a large genetic diversity. The cultivars were clustered into three groups: (1) spring rapeseed of European and Northern American origin, (2) winter rapeseed of European and Northern American origin, and (3) rapeseed of Asian origin. Several of the resynthesized rapeseed lines were similar to European winter rapeseed cultivars, whereas others had quite unique patterns. It is concluded, that resynthesized rapeseed is a valuable source for broadening the genetic variation in present breeding material of Brassica napus. However, different lines differ widely in their suitability for this purpose.
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    Theoretical and applied genetics 91 (1995), S. 439-447 
    ISSN: 1432-2242
    Keywords: Chrysanthemum ; RAPD ; DNA fingerprint ; RFLP ; Stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several techniques of DNA analysis were applied to identify chrysanthemum cultivars. Unrelated cultivars could be distinguished by using RAPDs (random amplified polymorphic DNAs), inter-SSR (simple sequence repeat) PCR (polymerase chain reaction), hybridization-based DNA fingerprinting, as well as RFLPs (restriction fragment length polymorphisms). Cultivars with different flower colours and belonging to one family, i.e. vegetatively derived from 1 cultivar, appeared to have the same DNA fragment patterns, whichever technique was applied. The absence of polymorphisms between different accessions of the same cultivar indicated a high stability of the observed patterns.
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    Theoretical and applied genetics 91 (1995), S. 947-955 
    ISSN: 1432-2242
    Keywords: RFLP ; Genetic map ; Polyploids ; Tall fescue ; Molecular marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic mapping using molecular markers such as restriction fragment length polymorphisms (RFLPs) has become a powerful tool for plant geneticists and breeders. Like many economically important polyploid plant species, detailed genetic studies of hexaploid tall fescue (Festuca arundinacea Schreb.) are complicated, and no genetic map has been established. We report here the first tall fescue genetic map. This map was generated from an F2 population of HD28-56 by ‘Kentucky-31’ and contains 108 RFLP markers. Although the two parental plants were heterozygous, the perennial and tillering growth habit, high degree of RFLP, and disomic inheritance of tall fescue enabled us to identify the segregating homologous alleles. The map covers 1274 cM on 19 linkage groups with an average of 5 loci per linkage group (LG) and 17.9 cM between loci. Mapping the homoeologous loci detected by the same probe allowed us to identify five homoeologous groups within which the gene orders were found to be generally conserved among homoeologous chromosomes. An exception was homoeologous group 5, in which only 2 of the 3 homoeologous chromosomes were identified. Using 12 genome-specific probes, we were able to assign several linkage groups to one of the three genomes (PG1G2) in tall fescue. All the loci detected by the 11 probes specific to the G1 and/or G2 genomes, with one exception, identified loci located on 4 chromosomes of two homoeologous groups (LG2a, LG2c, LG3a, and LG3c). A P-genome-specific probe was used to map a locus on LG5c. Comparative genome mapping with maize probes indicated that homoeologous group 3 and 2 chromosomes in tall fescue corresponded to maize chromosome 1. Difficulties and advantages of applying RFLP technology in polyploids with high levels of heterozygosity are discussed.
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  • 44
    ISSN: 1432-2242
    Keywords: Brassica napus ; RFLP ; Erucic acid genes ; Oil content ; QTL mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A F1 microspore-derived DH population, previously used for the development of a rapeseed RFLP map, was analysed for the distribution of erucic acid and seed oil content. A clear three-class segregation for erucic acid content could be observed and the two erucic acid genes of rapeseed were mapped to two different linkage groups on the RFLP map. Although the parents of the segregating DH population showed no significant difference in seed oil content, in the DH population a transgressive segregation in oil content was observed. The segregation closely followed a normal distribution, characteristic of a quantitative trait. Using the program MAPMAKER/QTL, three QTLs for seed oil content could be mapped on three different linkage groups. The additive effects of these QTLs explain about 51% of the phenotypic variation observed for this trait in the DH population. Two of the QTLs for oil content showed a close association in location to the two erucic acid genes, indicating a direct effect of the erucic acid genes on oil content.
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  • 45
    ISSN: 1617-4623
    Keywords: Wheat ; Rice ; Maize ; RFLP ; Synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of RFLP linkage maps in hexaploid and diploid oat allows us to study genetic relationships of these species at the DNA level. In this report, we present the extension of a previously developed diploid oat map (Avena atlantica x A. hirtula) and its molecular-genetic relationships with wheat, rice and maize. Examination of 92–99% of the length of the oat genome map with probes common to Triticeae species, rice or maize showed that 84, 79 and 71%, respectively, was conserved between these species and oat. Generally, the orders of loci among chromosomes homoeologous to oat chromosomes A and D were the most conserved and those of chromosomes homoeologous to oat chromosome G were the least conserved. Conservation was observed for blocks ranging from whole chromosomes 101 cM long to small segments 2.5 cM long containing two loci. Comparison of the homoeologous segments of Triticeae, rice and maize relative to oat indicated that certain regions have been maintained in all four species. The relative positions of major genes governing traits such as seed storage proteins and resistance to leaf rusts have been conserved between cultivated oat and Triticeae species. Also, the locations of three vernalization/or photo-period response genes identified in hexaploid oat correspond to the locations of similar genes in homoeologous chromosomes of wheat, rice or maize. The locations of the centromeres for six of the seven oat chromosomes were estimated based on the homoeologous segments between oat and Triticeae chromosomes.
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  • 46
    ISSN: 1617-4623
    Keywords: Oat ; Rice ; Maize ; RFLP ; Synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Conventionally, the genetics of species of the family Gramineae have been studied separately. Comparative mapping using DNA markers offers a method of combining the research efforts in each species. In this study, we developed consensus maps for members of the Triticeae tribe (Triticum aestivum, T. tauschii, andHordeum spp.) and compared them to rice, maize and oat. The aneuploid stocks available in wheat are invaluable for comparative mapping because almost every DNA fragment can be allocated to a chromosome arm, thus preventing erroneous conclusions about probes that could not be mapped due to a lack of polymorphism between mapping parents. The orders of the markers detected by probes mapped in rice, maize and oat were conserved for 93, 92 and 94% of the length of Triticeae consensus maps, respectively. The chromosome segments duplicated within the maize genome by ancient polyploidization events were identified by homoeology of segments from two maize chromosomes to regions of one Triticeae chromosome. Homoeologous segments conserved across Triticeae species, rice, maize, and oat can be identified for each Triticeae chromosome. Putative orthologous loci for several simply inherited and quantitatively inherited traits in Gramineae species were identified.
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  • 47
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    Plant systematics and evolution 196 (1995), S. 195-206 
    ISSN: 1615-6110
    Keywords: Pteridophyta ; Dryopteridaceae ; Dryopteris filix-mas ; Clonal gametophytes ; growth ; reproduction ; genetic and environmental effects ; phenotypic plasticity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Artificially formed clonal gametophytes ofDryopteris filix-mas (L.)Schott were able to grow and reproduce, although growth rates, proportions of hermaphrodites and reproductive efforts were low. Variable density and nutrient levels appeared to affect gametophytic life histories and they continued to influence the viability of the developing sporophyte generation. Differences between populations and sporophytes were discovered in life histories. Hermaphroditic gametophytes were considerably larger and possessed higher viability than did males. Clear differences between clones in the level of phenotypic plasticity in gametophyte size were observed. No neighbor effect on gametophyte sex expression was detected with the densities and nutrient levels used.
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  • 48
    ISSN: 1573-5125
    Keywords: growth ; Azolla ; eutrophication ; mercury ; India
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth ofAzolla was stimulated in different water bodies in Burdwan. It depended on the PO4−P contents of the media. Biomass, chlorophyll and tissue phosphorus content ofAzolla were positively correlated with PO4−P of the different water bodies while chlorophyll content of the fern was positively correlated with conductivity. We concluded that the water bodies of Burdwan can be utilised as sites for large scale multiplication ofAzolla for fertilization purposes in rice cultivation, despite the presence of mercury.
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  • 49
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    Molecular breeding 1 (1995), S. 273-282 
    ISSN: 1572-9788
    Keywords: bias ; doubled-haploid ; QTL reliability ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Several statistical methods which employ multiple marker data are currently available for the analysis of quantitative trait loci (QTL) in experimental populations. Although comparable estimates of QTL location and effects have been obtained by these methods, using simulated and real data sets, their accuracy and reliability have not been extensively investigated. The present study specifically examines the merit of using F2 and doubled haploid populations for locating QTL and estimating their effects. Factors which may affect accuracy and reliability of QTL mapping, such as the number and position of the markers available, the accuracy of the marker locations and the size of the experimental population used, are considered. These aspects are evaluated for QTL of differing heritabilities and locations along the chromosome. A population of 300 F2 individuals and 150 doubled haploid lines gave estimates of QTL position and effect which were comparable, albeit extremely unreliable. Even for a QTL of high heritability (10%), the confidence interval was 35 cM. There was little increase in reliability to be obtained from using 300, rather than 200, F2 individuals and 100 doubled haploid lines gave similar results to 150. QTL estimates were not significantly improved either by using the expected, rather than the observed, marker positions or by using a dense map of markers rather than a sparse map. A QTL which was asymmetrically located in the linkage group resulted in inaccurate estimates of QTL position which were seriously biassed at low heritability of the QTL. In a population of 300 F2 individuals the bias increased from 4 cM to 20 cM, for a QTL with 10% and 2% heritability respectively.
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  • 50
    ISSN: 1573-5176
    Keywords: microalgae ; temperature ; growth ; total lipids ; fatty acids ; tropical mariculture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of temperature from 10 °C to 35 °C on the growth, total lipid content, and fatty acid composition of three species of tropical marine microalgae, Isochrysis sp., Nitzschia closterium, N. paleacea (formerly frustulum), and the Tahitian Isochrysis sp. (T.ISO), was investigated. Cultures of N. closterium, Isochrysis sp. and T.ISO grew very slowly at 35 °C, while N. closterium did not grow at temperatures higher than 30 °C or lower than 20 °C. N. paleacea was low-temperature tolerant, with cells growing slowly at 10 °C. N. paleacea produced the highest percentage of lipids at 10 °C, while the other species produced maximum amounts of lipid at 20 °C. None of the species maintained high levels of polyunsaturated fatty acids (PUFAs) at high growth temperature and there was a significant inverse relationship between the percentage of PUFAs and temperature for N. paleacea. A curved relationship was found between temperature and percentage of PUFA for N. closterium and tropical Isochrysis sp., with the maximum production of PUFA at 25 °C and 20 °C, respectively. The two Nitzschia species produced higher levels of the essential fatty acid eicosapentaenoic acid [20:5(n-3)] at lower growth temperatures, but the two Isochrysis species had little change in percentage of 20:5(n-3) with temperature. Only T.ISO had the highest percentage of 22:6(n-3) at lowest growth temperature (11.4% total fatty acids at 10 °C).
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  • 51
    ISSN: 1573-5117
    Keywords: grapsid crabs ; detritivory ; growth ; survival ; assimilation efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth performance of adult individuals of the detritivorous mangrove grapsid crabs Chiromanthes bidens and Parasesarma plicata common in the Mai Po marshes, Hong Kong, was followed in a long-term laboratory rearing experiment (10 months). Individual crabs' moulting frequency, growth increment and mortality when fed four kinds of leaf litter available in their natural habitats, viz. brown (two weeks of decomposition) and yellow (fresh litter) Kandelia candel, and brown and yellow Avicennia marina were followed. The survival period was strongly related to litter type when the data from both crab species were pooled, being longest for crabs fed with brown Avicennia marina, followed by brown Kandelia candel 〉 yellow Avicennia marina 〉 yellow Kandelia candel. A higher proportion of crabs moulted twice when yellow Avicennia marina was supplied, but more crabs moulted for a third time when fed with brown Avicennia marina. The growth increment of the two species of crabs after moulting was found to be significantly related to their pre-moult size (ANOVA, P〈0.05), but effects of the four treatments were non-significant. Increment of Chiromanthes bidens was significantly greater than that of Parasesarma plicata under the brown Kandelia candel treatment. Litter treatment has no effect on the time taken for the crabs to moult, only in the case of the yellow Avicennia marina treatment did Parasesarma plicata take a longer time to moult a second time.
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  • 52
    ISSN: 1573-5117
    Keywords: Mangrove ; Kandelia candel ; seedling ; growth ; wastewater ; pollution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A greenhouse experiment wad performed to evaluate the effects of synthethic wastewater in three different strengths, NW, MW and CW, on the growth of the one-year old Kandelia candel (L.) Druce plants. NW had the characteristics and strength similar to natural municipal wastewater while MW and CW contained five and ten times of the nutrients and heavy metals in NW, respectively. Artificial seawater was used as the control. During one year wastewater treatment experiment, Kandelia were found to withstand wastewater of high strength and toxic symptoms were not detected in all plants. Synthethic wastewater with strength similar to the natural municipal sewage (NW) stimulated plant growth. The plants treated with NW had significantly higher aerial and root biomass, taller stem than those found in the CW, MW and the control. Maximum growth, in terms of both stem height and total biomass, of all plants occured in summer months, from June to September. With respect to the physiological and biochemical activities, CW and MW treated plants had significantly lower levels of chlorophyll a, total chlorophyl and catalase activity than those found in NW and control groups. In contrast, the proline content of plants treated with wastewater was similar to that of the control. These result suggest that normal wastewater (NW), attributed to its nutrients and trace elements, enhanced plant growth. The medium (MW) and concentrated wastewater (CW) supported similar amount of plant growth as the control but the physiological and biochemical parametes indicate that these treated plants might have been exposed to some kind of stress, probably due to the heavy metals present in MW and CW.
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  • 53
    ISSN: 1573-5117
    Keywords: β-carotene ; baker's yeast ; growth ; survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Coloration of phyllopods varies from place to place and from one life stage to another. It ranges from translucent or whitish through gray, blue, green, orange, and reddish. Here, we present experimental evidence for a food- dependent color pattern inThamnocephalus platyurus Packard. The presence or absence of the synthetic pigment trans — β — carotene in a baker's yeast diet was the controlling factor. All the 24 old larvae used in the experiment were whitish in color. From day 6 until the end the experiment (day 11), 100% of the shrimps under a diet with synthetic trans — β — carotene (treatment 1) exhibited a characteristic color pattern which consisted of an orange color in the cercopods, and in all theracopods; the rest of the body exhibited no particular color. In comparison, 100% of the shrimps under a diet without synthetic trans — β — carotene (treatment 2) were whitish throughout the body. In females from treatment 1, the ovaries and oocytes were green-bluish, while in females from treatment 2 the ovaries and oocytes were whitish. No significant differences in survival and growth were found, except that at day 9, there was a significant difference in growth, the females with the synthetic trans — β — carotene group growing faster.
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  • 54
    ISSN: 1573-5117
    Keywords: Thamnocephalus ; Branchinecta ; growth ; filter-feeders ; feeding ; clay ; silicium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We designed and standardized a culture method for freshwater anostracans using diets free of live algae.Thamnocephalus platyurus andBranchinecta lindahli were used as test organisms. We used baker's yeast as basic food and added inert particles (clay or amorphic silicium dioxide) to improve the digestion of the yeast. A flow-through culture system was used, according to a fixed feeding schedule, to supply separately, culture medium (tap water), food, and inert particle suspensions. Three variants with baker's yeast as basic, food were compared for survival, growth, and reproduction. A diet of solely baker's yeast (diet 1) or baker's yeast supplemented with vegetal oil containing ß-carotene (diet 2) was unsuitable for reproduction ofT. platyurus. Cyst production was only achieved when diet 2 was supplemented with fish oil andSpirulina powder (diet 3). This suggests that not only a digestibility problem, but also nutritional deficiencies are present in baker's yeast.
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  • 55
    ISSN: 1573-5117
    Keywords: Anostraca ; algal food ; temperature ; conductivity ; water quality ; growth ; maturation ; survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Food concentration (0.5 × 103 − 5 × 105 Scenedesmus cells m1−1) significant influenced the somatic growth, maturation and survivorship ofS. proboscideus larvae. A density of 5 × 104 cells m1−1 was optimal for early larval stages. Of four temperature tested (20–35 °C), 30 °C resulted in the best growth and survival. Maturation time was inversely related to temperature, and was size- rather that age-dependent. Larvae were tolerant of a wide conductivity range, but optimal growth and survival were observed at 260 µS cm−1. Nitrate-Nitrogen (NO2-N) caused a larval mortality of 50% after 24 h at 0.58 mg1−1.
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  • 56
    ISSN: 1573-5117
    Keywords: Rutilus lemmingii ; age ; growth ; reproduction ; fecundity ; life-history tactics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The age, growth and reproduction ofRutilus lemmingii (Steindachner, 1866), an endemic cyprinid from the Iberian Peninsula, was studied for over a period of two years in a small seasonal tributary of the Guadalquivir river basin. Approximately 65 % of the total growth in length occured in the first year of life. Males reached a maximum age of 3+ yr (Fork Length, F.L. = 114 mm) and females 4+ yr (F.L. = 144 mm). Both sexes matured during their second year of life (1 +). The overall sex ratio (334 males to 389 females) differed significantly from unity. Somatic condition decreased markedly during the reproductive period of March to May.R. lemmingii is a multiple spawner and releases two batches of eggs per female each year. Mean egg diameter of the first batch was larger than the second one. The regression between fecundity and Fork Length (mm) was: Fec = 0.014 F.L.Z.2.858 Compared with available information, thisR. lemmingii population, located at a lower latitude, is characterized by fast growth, early maturity, high level of reproductive effort, and a short life-span. These life-history characteristices are typical of species in unstable environments, where adult mortality is high, variable or unpredictable.
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  • 57
    ISSN: 1573-5117
    Keywords: Alps ; prealpine streams ; Ephemeroptera ; community structure ; life cycle ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Within the framework of a study on the ecology of Dippers in a prealpine stream system, the macroinvertebrate communities were also investigated, especially Ephemeroptera. Ten stations were sampled qualitatively, of which two (Saane and Turbach streams) were investigated quantitatively for 16 months (April 1983–July 1984). Fifteen mayfly species were found. The faunistic affinities among the 10 streams investigated reflect the main abiotic characteristics. There was no significant difference in species richness, diversity and evenness for Saane and Turbach streams. Life cycles of nine species were studied in these two watercourses. Those of Rhithrogena degrangei, Rh. hybrida, Rh. savoiensis and Ecdyonurus helveticus are described for the first time. Baetis alpinus is the only species to exhibit a bivoltine cycle, whereas all others have a univoltine summer or winter cycle. Specific growth rates were measured for most species. The summer generation of B. alpinus presents the highest growth rate (4.51 % dry weight d−1), whereas the lowest values were recorded for Rh. degrangei (0.76% dry weight d−1). The importance of abiotic variables, especially water temperature, is also discussed.
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  • 58
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    Hydrobiologia 308 (1995), S. 153-165 
    ISSN: 1573-5117
    Keywords: water mite ; Piona ; growth ; development ; temperature-food interactions ; zooplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We analysed the relative effects of food availability and temperature on rates of growth and development of a predatory planktonic water mite, Piona exigua. Growth in length of mites fed Daphnia, Ceriodaphnia and Chydorus was analysed by Gompertz or von Bertalanffy curves; these curves were compared by parallel curve analysis. Growth rates of nymphs and adult female mites increased with temperature; the duration of the imagochrysalis stage decreased. Females grown at 10 °C were smaller at final size than females grown at 15 °C, 18 °C or 22 °C. Females reared at food levels of 15 or 30 prey l−1 grew more slowly and were smaller than those provided with 60 or 120 prey l−1. Nymphs grew more slowly when Daphnia were the only prey, than when smaller prey were available. Food level did not affect nymph growth at 10 °C or 15 °C, but growth at 18 °C or 22 °C may have been slowed at the lowest food levels. Synergistic effects of temperature and food level on nymph growth were apparent only from analysis of growth curves and not from stage duration data.
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  • 59
    ISSN: 1573-5168
    Keywords: larval herring ; protein synthesis ; growth ; energetics ; protein turnover ; protein synthesis costs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rates of growth, protein synthesis and oxygen consumption were measured in herring larvae, Clupea harengus, in order to estimate the contribution that protein synthesis makes to oxygen consumption during rapid growth at 8°C. Protein synthesis rates were determined in larvae 9 to 17 d after hatching. Larvae were bathed in 3H phenylalanine for several hours and the free pool and protein-bound phenylalanine specific radioactivities were determined. Fractional rates of protein synthesis increased 5 to 11 fold with feeding after a period of fasting. Efficiencies of retention of synthesized protein were approximately 50% during rapid growth. Rapid growth in herring larvae thus appears to be characterized by moderate levels of protein turnover similar to those obtained for larger fish. Increases in growth rate occurred without changes in RNA concentration, i.e., the larvae increased the efficiency of RNA rapidly. Oxygen consumption rates were not correlated with growth rates. Protein synthesis was estimated to account for 79% of the oxygen consumption, and energy costs of protein synthesis were high, i.e., about 98 mmole O2 g−1 protein synthesized.
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  • 60
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    Molecular genetics and genomics 248 (1995), S. 278-286 
    ISSN: 1617-4623
    Keywords: Flowering time ; Vernalization ; Quantitative trait loci ; Arabidopsis ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Quantitative trait loci (QTL) analyses based on restriction fragment length polymorphism maps have been used to resolve the genetic control of flowering time in a cross between twoArabidopsis thaliana ecotypes H51 and Landsbergerecta, differing widely in flowering time. Five quantitative trait loci affecting flowering time were identified in this cross (RLN1-5), four of which are located in regions containing mutations or loci previously identified as conferring a late-flowering phenotype. One of these loci is coincident with theFRI locus identified as the major determinant for late flowering and vernalization responsiveness in theArabidopsis ecotype Stockholm.RLN5, which maps to the lower half of chromosome five (between markers mi69 and m233), only affected flowering time significantly under short day conditions following a vernalization period. The late-flowering phenotype of H51 compared to Landsbergerecta was due to alleles conferring late flowering at only two of the five loci. At the three other loci, H51 possessed alleles conferring early flowering in comparison to those of Landsbergerecta. Combinations of alleles conferring early and late flowering from both parents accounted for the transgressive segregation of flowering time observed within the F2 population. Three QTL,RLN1,RLN2 andRLN3 displayed significant genotype-by-environment interactions for flowering time. A significant interaction between alleles atRLN3 andRLN4 was detected.
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  • 61
    ISSN: 1617-4623
    Keywords: Leaf rust ; RFLP ; Sequence-tagged-site (STS) ; Wheat ; Resistance gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this study was to characterize the leaf rust resistance locusLr1 in wheat. Restriction fragment length polymorphism (RELP) analysis was performed on the resistant lineLr1/6*Thatcher and the susceptible varieties Thatcher and Frisal, as well as on the segregating F2 populations. Seventeen out of 37 RFLP probes mapping to group 5 chromosomes showed polymorphism betweenLr1/6*Thatcher and Frisal, whereas 11 probes were polymorphic between the near-isogenic lines (NILs)Lr1/6*Thatcher and Thatcher. Three of these probes were linked to the resistance gene in the segregating F2 populations. One probe (pTAG621) showed very tight linkage toLr1 and mapped to a single-copy region on chromosome 5D. The map location of pTAG621 at the end of the long arm of chromosome 5D was confirmed by the absence of the band in the nulli-tetrasomic line N5DT5B of Chinese Spring and a set of deletion lines of Chinese Spring lacking the distal part of 5DL. Twenty-seven breeding lines containing theLr1 resistance gene in different genetic backgrounds showed the same band asLr1/6*Thatcher when hybridized with pTAG621. The RFLP marker was converted to a sequence-tagged-site marker using polymerase chain reaction (PCR) amplification. Sequencing of the specific fragment amplified from both NILs revealed point mutations as well as small insertion/deletion events. These were used to design primers that allowed amplification of a specific product only from the resistant lineLr1/6*Thatcher. This STS, specific for theLr1 resistance gene, will allow efficient selection for the disease resistance gene in wheat breeding programmes. In addition, the identification of a D-genome-specific probe tightly linked toLr1 should ultimately provide the basis for positional cloning of the gene.
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  • 62
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    Plant and soil 168-169 (1995), S. 313-317 
    ISSN: 1573-5036
    Keywords: growth ; internal cycling ; partitioning ; phosphorus ; 32P ; Sitka spruce
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The availability of phosphorus in many UK forest soils limits growth of Sitka spruce (Picea sitchensis (Bong.) Carr.). Efficient cycling of P within such systems is therefore necessary for sustained tree growth. Internal cycling of P is an important component of the overall P cycle in forests and the current work aims to quantify the impact of P nutrition on internal cycling and seasonal growth of Sitka spruce. Two-year old seedlings of Sitka spruce were grown in sand culture in the glasshouse for one year. Two treatments were imposed in which trees received either a complete nutrient solution from which P was excluded (-P) or one in which P was applied as labelled 32P (+P). Internal cycling of P was measured directly in plants which had received no P and by difference in those which received 32P. The contrasting P treatments produced an eight-fold difference in P content and a three-fold difference in tree growth between May and October. Root:shoot ratios increased during the growing season from 0.29 to 0.38 and from 0.29 to 0.52 in +P and-P treatments, respectively. In both treatments P was translocated from old shoots to support new shoot growth. P supply did not affect the amount of P remobilised but there was evidence that the rate of remobilisation may have been affected. The partition of remobilised P was affected by current P supply and differed from the partition of current P uptake. Results are compared to those from studies of growth and internal cycling of nitrogen in Sitka spruce.
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  • 63
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    Plant and soil 173 (1995), S. 299-310 
    ISSN: 1573-5036
    Keywords: atmospheric deposition ; balanced nutrition ; excess NH4 ; growth ; nutrient cycling ; Pinus sylvestris L. ; soil acidification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a Scots pine forest stand, demineralized water and a complete set of nutrients with water were applied to the soil by means of frequent irrigation for four years in order to eliminate water and nutrient shortage of the trees. Apart from this optimization, dissolved (NH4)2SO4 was irrigated at a rate of 120 kg N ha-1 y-1 to create a situation of N excess. Effect of treatments on tree growth and chemical composition of soil water and vegetation were monitored. From the first treatment year onwards basal area growth increased by ca. 35% as a result of the increased water supply. Nutrient applications increased K and P concentrations in pine needles immediately, but growth was enhanced only in the fourth treatment year and coincided with an improved K supply. Most of the applied P and K was retained in the soil, and only 6% was recovered in the vegetation. Tree nutrient status did not respond on Ca and Mg applications, whereas Ca and Mg seepage losses were increased with ca. 5 kg ha-1 y-1. The applied NH4 was mostly retained in the 0–20 cm surface soil and caused a drastic increase of Al in soil solution. Tree growth was stimulated initially by extra NH4, but was hampered after three years obviously because of a decreased P nutrition. The applied base cations were absorped to the soil and the accompanying anions were leached, thus temporarily increasing the acidification of the soil solution.
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  • 64
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    Plant and soil 176 (1995), S. 183-187 
    ISSN: 1573-5036
    Keywords: banana ; Glomus sp. ; growth ; nutrition ; relative mycorrhizal dependency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seven banana cultivars (Musa acuminata, AAA group) were inoculated with two species of vesicular arbuscular mycorrhizal (VAM) fungi (Glomus mosseae and Glomus macrocarpum) in a greenhouse experiment. Inoculated plants had generally greater shoot dry weight and shoot phosphorus concentrations compared to the noninoculated plants. A great variation in dependency on mycorrhizal colonization was observed among the banana cultivars. Cv. Williams showed the highest relative mycorrhizal dependency (RMD) and cv. Poyo the lowest. For all the cultivars studied, inoculation with G. macrocarpum resulted in the highest RMD values. Both root dry weight and root hair length or density of the noninoculated plants were inverserly correlated with the RMD values of cultivars.
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  • 65
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    Plant cell, tissue and organ culture 42 (1995), S. 179-184 
    ISSN: 1573-5044
    Keywords: growth ; leaf anatomy ; PEG ; Vitis sp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyethylene glycol was used to induce water stress of micropropagated ‘Valiant’ grape. Reduced growth and slow rooting were observed in treated plantlets with 2, 4 and 6% polyethylene glycol as compared to control plantlets with no polyethylene glycol in the rooting medium. At high concentrations of 4 and 6%, leaves exhibited wilting and necrosis. At the 2% level, plantlets recovered and grew satisfactorily. Detached leaves of treated plantlets with 2% polyethylene glycol lost less water than controls when exposed to low humidity for 4 hours. Leaf anatomy of plantlets treated with 2% polyethylene glycol, control (in vitro plantlets) and greenhouse-grown plants were compared under light microscopy. Leaves from control plantlets contained larger mesophyll cells, lacked normal palisade layer formation, had greater intercellular pore spaces and fewer chloroplasts. Leaves from polyethylene glycol-treated plantlets, however, had smaller mesophyll cells, a more defined palisade layer, reduced intercellular pore space and the greatest number of chloroplasts. These results suggest that an osmoticum such as polyethylene glycol may be used to induce more normal leaf anatomy and reduced water loss in micropropagated ‘Valiant’ grapes.
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  • 66
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    Euphytica 86 (1995), S. 103-110 
    ISSN: 1573-5060
    Keywords: Korean weedy rice ; Oryza sativa ; RFLP ; classification ; weedy rice ; genetic similarity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genetic characteristics and classification of 24 strains of Korean weedy rice, two strains of foreign red rice, three Japonica cultivars, one Tongil cultivar and one Indica cultivar (Oryza sativa L.) were investigated at the DNA level using the restriction fragment length polymorphism (RFLP) method. Eighty-three random combinations between six restriction enzymes and forty genomic DNA probes (RG# and KR#) were assayed. Thirty-seven (92.5%) out of the forty probes used showed polymorphisms among the 31 accessions assayed. A high level of polymorphism was found between short and long grain type Korean weedy rices, whereas fewer polymorphisms were presented among strains within each grain type. A dendrogram summarizing genetic similarity coefficients among thirty-one accessions was constructed based on their DNA polymorphisms. The Korean weedy rice strains were classified into two groups identical to the short and long grain types classified by morphological and physiological characters. From the RFLP analysis, it was deduced that the short grain strains of Korean weedy rice belonged to Japonica, while the long grain strains were closer to Indica than to Japonica, and were differentiated into a local ecotype surviving in the growth conditions in the southern part of the Korean peninsula.
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  • 67
    ISSN: 1573-5036
    Keywords: fertilization ; growth ; maple decline ; nutrient status
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A five year study was conducted in a 100–120 year old even-aged sugar maple stand in southern Quebec (46°07′N 73° 56′W; 305 m altitude) to explore the effect of different fertilization formulations aimed at 1) correcting the most common nutrient deficiencies observed in declining maple stands (K and Mg), 2) decreasing soil acidity, and 3) simulating enrichment with atmospheric N. Seven fertilizer mixtures were applied in the spring of 1987: 400 kg ha-1 of K2SO4, CaCO3, CaMg(CO3)2, (NH4)2SO4, complete fertilizer (“Maplegro”) and 800 kg ha-1 of an equal mixture of K2SO4+CaCO3 or K2SO4+CaMg(CO3)2. The site was divided into twenty-four 25×25 m plots and treatments including control were replicated three times. Leaves and soils (organic and mineral) were sampled in 1987, 1988 and 1991. Trees were cored at 1.2 m to measure their response in diameter growth. The application of K2SO4+CaMg(CO3)2 was the only treatment that significantly increased (+13%) the average growth rate over the five year period after fertilization. The application of (NH4)2SO4, “Maplegro”, CaMg(CO3)2 and K2SO4 reduced growth relative to the control for the five year period by 29, 24, 20 and 12 %, respectively. Positive and negative effects on growth can be explained mainly in terms of changes in leaf K. Both the application of Maplegro and (NH4)2SO4 increased soil P availability. Overall, the rate of growth showed a cubic pattern of change over the 5 year period with peaks in 1988 and 1991. Trees in control plots went from a limiting foliar status of Ca and Mg, and surplus N in 1987 to a surplus of Ca and Mg, and lower N concentration in 1991. Our results suggest that nutrient deficiencies observed at our site were associated with a disturbance of the biogeochemical cycle of nutrients rather than soil nutrient depletion.
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  • 68
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    Plant and soil 168-169 (1995), S. 15-20 
    ISSN: 1573-5036
    Keywords: growth ; nutrition ; reference values ; relative addition/uptake/growth rate ; steady-state
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Strictly controlled experiments with plants, acclimatized under steady-state conditions and grown for a sufficiently long time period to get reliable and representative measurements, are necessary to obtain plant responses in precise terms (reference values). It is then possible to reproduce and compare experimental results with a high accuracy and to establish fundamental plant properties in an unambiguous and unifying terminology. Two kinds of growth determining factors can be distinguished: 1) Mass transport, i.e. variables that express flux rates of carbon and mineral nutrients in relation to plant size and requirements (relative addition and uptake rates). These factors influence the relative growth rate, one at a time (Liebig's law of the minimum). 2) Factors that influence the mass transports, i.e. non-elemental resources, e.g. light and water availability, and modulators, e.g. genome, nutrient status and temperature. These factors interact in orthogonal relationships with each other and can be specified in normalized terms when the optimum value is known.
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  • 69
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    Plant and soil 176 (1995), S. 101-105 
    ISSN: 1573-5036
    Keywords: arbuscular mycorrhiza ; “criollo” ; growth ; inoculation ; maize ; mycorrhizal ; native ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We tested the effect of two single species inocula and a mixed inoculum of the native population of arbuscular mycorrhizal (AM) fungi on the growth response of “criollo” maize (Zea mays L.). To determine the inocula that produced the highest response on maize growth, we conducted a greenhouse experiment at 3 levels of P fertilization (0, 40 and 80 kg ha−1). Inoculation with Glomus mosseae (Nicolson and Gerdemann) Gerd. and Trappe (LMSS) produced the greatest shoot growth rates at the two lowest P fertilization levels. Inoculation with Acaulospora bireticulata Rothwell and Trappe (ABRT) and the native population (NP) resulted in similar shoot growth rates at all P levels. These rates were higher than the non-mycorrhizal control rate at the lowest P level but lower than the control at the highest P level. Also, ABRT and NP had significantly lower shoot growth rates than the inoculation treatment with G. mosseae at all P levels. The non-mycorrhizal control had the lowest growth rate at the lowest P level but its growth rate increased linearly with increased P fertilization. Inoculation with G. mosseae and A. bireticulata produced similar colonization rates which were lower than the native population colonization rate. There was no correlation between colonization and shoot growth rates.
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  • 70
    ISSN: 1573-5036
    Keywords: growth ; nutrient availability ; Pinus strobus ; Picea glauca ; site preparation ; soil organic carbon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influences of soil surface modification (blade scarification and plastic mulching), fertilization and herbicide application on soil nutrient and organic carbon content and tree growth and foliar nutrient status were examined after seven years in a study located within the Great Lakes-St. Lawrence forest region of Canada. Plots had been planted with white pine (Pinus strobus L.) and white spruce (Picea glauca [Moench] Voss) seedlings. Light (PAR), soil moisture and temperature were monitored and recorded throughout the growing season. Forest floor and soil mineral (0–20 cm layer) samples were collected from all experimental plots, except those which had plastic mulching. Foliar samples were collected in autumn and analysed for N, P and K and storage compounds. Seedling mortality was 20% higher in unscarified plots. Combined silvicultural treatments increased productivity as much as 14 times, but scarification reduced soil carbon and nutrient capital 2–3 fold. Herbicide application reduced soil carbon by at least 20 %. Foliar nutrient, protein, starch and lipid contents in autumn were little affected by treatment. The future management of such stands in Canada probably will include more shelterwood harvesting and crop rotations, silvicultural systems that are more closely aligned with natural forest succession.
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  • 71
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    Plant and soil 168-169 (1995), S. 535-545 
    ISSN: 1573-5036
    Keywords: CO2 ; global change ; growth ; nitrogen ; nutrition ; Pinus ponderosa ; soil nutrients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper reports on the results of a controlled-environment study on the effects of CO2 (370, 525, and 700 μmol mol-1) and N [0, 200, and 400 μg N g soil-1 as (NH4)SO4] on ponderosa pine (Pinus ponderosa) seedlings. Based upon a review of the literature, we hypothesized that N limitations would not prevent a growth response to elevated CO2. The hypothesis was not supported under conditions of extreme N deficiency (no fertilizer added to a very poor soil), but was supported when N limitations were less severe but still suboptimal (lower rate of fertilization). The growth increases in N-fertilized seedlings occurred mainly between 36 and 58 weeks without any additional N uptake. Thus, it appeared that elevated CO2 allowed more efficient use of internal N reserves in the previously-fertilized seedlings, whereas internal N reserves in the unfertilized seedlings were insufficient to allow this response. Uptake rates of other nutrients were generally proportional to growth. Nitrogen treatment caused reductions in soil exchangeable K+, Ca2+, and Mg2+ (presumably because of nitrification and NO3 - leaching) but increases in extractable P (presumably due to stimulation of phosphatase activity). The results of this and other seedling studies show that elevated CO2 causes a reduction in tissue N concentration, even under N-rich conditions. The unique response of N is consistent with the hypothesis that the efficiency of Rubisco increases with elevated CO2. These results collectively have significant implications for the response of mature, N-deficient forests to evevated CO2.
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  • 72
    ISSN: 1573-5036
    Keywords: Acetobacter ; Herbaspirillum ; growth ; identification ; PCR ; sugarcane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nitrogen-fixing Acetobacter diazotrophicus, Herbaspirillum seropedicae and Herbaspirillum rubrisubalbicans colonize sugar cane, and are thought to be capable of supplying high levels of fixed nitrogen to this plant. Eight A. diazotrophicus, two H. seropedicae and four H. rubrisubalbicans isolates were identified and compared by complementary biochemical and genetic methods. Utilization of carbon sources and antibiotic resistance patterns allowed differentiation of A. diazotrophicus from Herbaspirillum species. In order to distinguish strains within A. diazotrophicus species, the polymerase chain reaction was employed, using a Rhizobium meliloti dctA primer under low stringency hybridization conditions.
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  • 73
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    Plant and soil 176 (1995), S. 307-316 
    ISSN: 1573-5036
    Keywords: chelate ; deficiency ; genotypic differences ; growth ; screening ; Triticum ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ten Triticum aestivum and two Triticum turgidum conv. durum genotypes were grown in chelate-buffered nutrient solution at Zn supplies ranging from deficient to sufficient (free Zn activities from 2 to 200 pM, pZn from 11.7 to 9.7). The critical level of Zn ion activity in solution for healthy growth of wheat plants was around 40 pM. Genotypes differed in the growth response: those classified as Zn-efficient suffered less reduction of shoot growth and did not change the rate of root growth at a Zn supply quite deficient for Zn-inefficient genotypes. Root growth of Zn-inefficient genotypes increased at deficient Zn supply. The shoot/root ratio was the most sensitive parameter of Zn efficiency; Zn-efficient genotypes showed less reduction in the ratio when grown at deficient compared to sufficient Zn supply. Classification of wheat genotypes into Zn-efficient and Zn-inefficient groups after screening in chelate-buffered nutrient solution corresponded well with classification obtained in field experiments on Zn-deficient soil.
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  • 74
    ISSN: 1573-5095
    Keywords: broadcast application ; cost effectiveness ; growth ; hexazinone ; spot application ; survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The herbicide, hexazinone, was applied four ways over ponderosa pine, 2–0 seedlings planted in northeast Oregon. The four treatments were two broadcast applications, a single broadcast application, a large spot application, and a small spot application. Seedling survival and growth were monitored for five growing seasons. Results indicate that survival more than doubled with either large or small spot applications compared to no application, and either one or two broadcast applications can increase survival an additional 30% over spot applications. Differences in stem volume were substantial, with two broadcast applications yielding more than twice the volume of a single broadcast treatment and more than five times the volume of seedlings treated with spot applications. Trees in small spots were still three times bigger than surviving seedlings in the control. These results are consistent with the concept of competition threshold. Management implications were considered in terms of cost of established seedlings. Although broadcast applications cost more per acre than spot applications, gains in seedling survival, growth and quality offset the additional cost and translate into lower established seedling costs. The cost effectiveness of broadcast applications also may be seen in the elimination of replanting or in-planting requirements and increases in long-term growth potential of the established trees.
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  • 75
    ISSN: 1573-5117
    Keywords: Daphnia longispina ; humic lakes ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The availability and importance of food sources for growth of Daphnia longispina L. from a highly coloured fishless lake with anoxic hypolimnion were assessed by combining in situ and laboratory experiments. In in situ experiments populations were enclosed in tubes with natural temperature stratification and with or without anoxic hypolimnion. In the laboratory experiments the importance of food source (littoral zone vs pelagic epilimnion) was assessed by enclosing moss thalli and a natural zooplankton population in a large-scale flow-through system supplying food for experimental Daphnia. Growth of juveniles of Daphnia in epilimnetic water was determined in batch culture experiments and the importance of increasing concentrations of bacteria and algae for their growth and development was investigated with a small-scale flow-through system. Access to the anoxic hypolimnion enhanced the growth of Daphnia by 23–24%. Growth rates in the tubes with anoxic hypolimnion were 0.36 and 0.16 d−1 in July and August respectively. In tubes without anoxia the corresponding values were 0.29 and 0.13. In batch-cultures the highest growth rate determined was 0.16 and the overall rates were lower than in in situ experiments. In batch culture Daphnia was able to grow in darkness for 10 days with a rate of 0.16. In the large-scale flow-through system Daphnia population fed with littoral water reproduced well despite the low concentration of algae and increased its number by a factor of c. 32 in 10 days. However, the animals were small and net production of Daphnia population thus lower under the littoral influence than in the control treatment. Population could survive and grew slowly on pelagial water processed by a natural zooplankton community and with very little algae left. It is thus possible that bacteria serve as a ‘life-support system’ enabling the population survival over periods of algal shortage. Small-scale flow-through experiments revealed that Daphnia longispina is able to mature and reproduce on a bacterial diet if the food concentration is high enough and Daphnia on bacterial food could achieve growth rates similar to those on an algal diet. The threshold food level for Daphnia longispina was estimated to be c. 18.5 μg C 1−1. Detrital material is of limited value in nutrition of Daphnia even in a lake where more than 75% of carbon is bound in particulate detritus.
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  • 76
    ISSN: 1573-5168
    Keywords: turbot ; growth ; γ-linolenic acid ; eicosapentaenoic acid ; liver pathology ; lipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A marine fish oil, Marinol K (MO) and borage oil (BO) were used to formulate diets relatively rich in eicosapentaenoic acid [EPA; 20:5(n-3)] and γ-linolenic acid [GLA; 18:3(n-6)], respectively. The diets were fed to duplicate groups of juvenile turbot (Scophthalmus maximus) of initial weight 1.4 g for a period of 12 weeks. No differences were observed in final weights either between duplicate tanks or between dietary treatments. Mortalities in the MO-fed group were significantly greater than in the BO-fed group. In the MO-fed group, 7 out of 12 fish sampled for histological analysis showed a pronounced liver histopathology whereas only 1 of 12 fish sampled in the BO-fed group showed slight pathology. EPA levels were increased 2.2-fold and its elongation product, 22:5(n-3), was increased 1.8-fold while arachidonic acid [AA; 20:4(n-6)] was decreased by 30% in MO-fed fish compared to the initial carcass composition. GLA was increased 53-fold and its elongation product dihomo-γ-linolenic acid [DHGLA; 20:3(n-6)] was increased 16-fold while AA was reduced by 90% in BO-fed fish compared to the initial carcass composition. The amount of triacylglycerol in liver of BO-fed fish was significantly greater than levels in MO-fed fish. The fatty acid compositions of individual phospholipids from liver showed marked differences between dietary treatments. Fish fed MO had significantly higher levels of the (n-3) polyunsaturated fatty acids (PUFA), 20:5(n-3), 22:5(n-3) and 22:6(n-3), and also significantly more 20:4(n-6) compared to BO-fed fish which had significantly higher 18:2(n-6), 18:3(n-6), 20:2(n-6) and 20:3(n-6). The composition of liver phosphatidylinositol was particularly unusual in BO-fed fish having DHGLA as the major C20 PUFA which was 2.2-fold greater than AA and 3.9-fold greater than EPA. This study demonstrates that the carcass composition of turbot can be altered, by means of dietary lipids, to contain increased levels of EPA and DHGLA which would be of potential benefit in human as well as in fish nutrition. However, caution should be exercised when using very highly unsaturated oils relatively rich in EPA which may generate histopathological lesions in the fish.
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    Review of industrial organization 10 (1995), S. 579-588 
    ISSN: 1573-7160
    Keywords: Innovation ; profitability ; growth ; firm size ; R&D
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract A new data base measuring company-level innovative activity is used to test how firm growth, profitability, size, and R&D intensity influence subsequent innovative activity. While R&D intensity is found to promote subsequent innovations, and smaller firms are identified as being more conducive to innovation activity than are larger firms, we find that the effect of company growth and profitability on subsequent innovation depends on the technological-opportunity environment. Profitability is found to promote subsequent innovative activity for firms in high-technological-opportunity industries but not in low-technological-opportunity industries. By contrast, high growth generates more innovative activity for firms in low-technological-opportunity industries, but not in high-technological-opportunity environments.
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    Plant growth regulation 16 (1995), S. 271-278 
    ISSN: 1573-5087
    Keywords: gibberellins ; growth ; heterosis ; hybrid vigor ; metabolism ; Sorghum bicolor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The correlation between gibberellin (GA) metabolism and growth rate was investigated using two Sorghum bicolor inbred lines, Hegari and AT×623, and their heterotic F1 hybrid. Previous studies have demonstrated that this hybrid is taller and has substantially greater shoot dry weights and leaf areas than either parental inbred. [3H]GA20 was applied to the leaf whorl of seedlings and after 24 hours, plants were harvested and separated into roots, shoot cylinders containing the apical meristems, and leaf blades. Chromatographic analyses of metabolites indicated the conversions of [3H]GA20 to [3H]GA1,8 and 29. The conversion of [2H]GA20 to [2H]GA1 was demonstrated by gas chromatography-selected ion monitoring (GC-SIM). Putative glucosyl conjugates of all of the [3H]GAs were also produced and GA8 was identified by GC-SIM following enzymic cleavage of the putative [3H]GA8 glucosyl conjugate fraction. Comparing the genotypes, [3H]GA20 metabolism was more rapid in the shoot cylinders of the hybrid than in the shoot cylinders from inbreds. In the hybrid samples, there was a three-fold increase in the putative conjugate(s) of [3H]GA1 which was the principal metabolite, and increased production of [3H]GA8 and the putative conjugates of [3H]GA29 and [3H]GA8. Conversely, levels of the remaining precursor, [3H]GA20, and its putative conjugate(s) were reduced in the hybrid. The rate of GA20 metabolism was thus positively correlated with growth rate across these sorghum genotypes. This correlation supports a promotive role of GA in the regulation of shoot growth and in the expression of heterosis (hybrid vigor) in sorghum.
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  • 79
    ISSN: 1573-5117
    Keywords: heavy metals ; diet ; detritivory ; growth ; reproduction ; Potamopyrgus jenkinsi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Evidence for the influence of food type and heavy metals on shell growth and fertility is presented for a freshwater population of the snail P. jenkinsi. When fed an excess of lettuce or lamb heart (protein source), growth rates were higher for lettuce. Highest growth rates occurred at a diet of lettuce plus lamb heart. Fertility was favoured by a diet of lamb heart. When fed an excess of lettuce, the EC50 growth values were 16 µg Cd l−1, 13 µg Cu l−1, and 103 µg Zn l−1 in lake water; snail fertility was inhibited at 25 µg Cd l−1 and 30 µg Cu l−1. A diet of lake detritus spiked with Cd or Cu resulted in a decrease of approximately 50% in growth rates, when compared with growth on non-spiked detritus. Spiked detritus lost metals into lake water. Food type positively interacted with metal stress, both for growth rate and fertility. The assessment of inhibitory effects of detritus contaminated either in the field or, notably, by spiking, and serving as food source for deposit feeders is hampered by sampling problems in the field and by redistribution processes of pollutants between particles and water in laboratory-scale experiments.
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    World journal of microbiology and biotechnology 11 (1995), S. 244-244 
    ISSN: 1573-0972
    Keywords: Cassava ; extract ; fungi ; growth ; soya bean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A medium that is cheaper than commercial media but just as good for assessing growth and viability of yeasts and fungi has been formulated using local ingredients: cassava and soya beans.
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    Antonie van Leeuwenhoek 67 (1995), S. 363-370 
    ISSN: 1572-9699
    Keywords: riboprinting ; ribotyping ; RFLP ; 18SrDNA ; 25SrDNA ; ITS1 ; ITS2 ; Saccharomyces ; yeast phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Type strains of 10 genotypically distinctSaccharomyces species are differentiated by ribosomal DNA restriction fragment analysis (ribotyping). The full length of the chromosomal ribosomal repeat was amplified in two parts, the 18SrDNA including both ITS regions (2600 bp) and the 25SrDNA (3300 bp). Restriction fragments generated by 9 enzymes from these two products yield characteristic patterns, by which unknownSaccharomyces isolates are assigned to the type strains. For convenient separation and detection only fragments longer than 200 bp were monitored. In contrast to molecular differentiation methods of highest resolution as RAPD-PCR or fingerprinting, the results from ribotyping are absolutely reproducible and thereby suitable for databases. The phylogeny computed from the discrete character matrix for presence/absence of fragments by the PHYLIP program package is in complete accordance to the phylogeny derived from ribosomal RNA sequence analysis. By this the field of application of the long range ribotyping can be regarded basically as equal to DNA sequence analysis of the same locus. Because distant relationships are recognized, missidentified genera were detected upon the species assignment. This cannot be done by methods of higher resolution like RAPD-PCR or fingerprinting.
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    Antonie van Leeuwenhoek 68 (1995), S. 35-42 
    ISSN: 1572-9699
    Keywords: black yeasts ; Exophiala ; Wangiella ; Phaeococcomyces ; PCR-ribotyping ; RFLP ; PCR ; ribosomal repeat ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Portion of the ribosomal repeat of the type strains of the generaExphiala andPhaeococcomyces were subjected to RFLP analysis. The amplicon length of the small subunit rRNA, the fragment NS1-NS24, was found to vary between 1800 to 3200 nucleotides. In contrast, the length of the fragment ITS1-ITS4 comprising the internal transcribed spacers (ITS1 and ITS2) was found to be constant at 600 nucleotides. Analysis of restriction profiles confirmed the synonymy ofExophiala dermatitidis andMycotorula schawii. Torula bergeri andSporotrichum gougerotii were found to be identical toPhaeoannellomyces elegans, but different from their alleged synomymE. castellanii. A phenogram is presented.
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    Antonie van Leeuwenhoek 68 (1995), S. 293-296 
    ISSN: 1572-9699
    Keywords: Anaerobic degradation ; growth ; propionate ; sulfate reduction ; syntrophy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The syntrophic propionate-oxidizing bacterium MPOB was able to grow in the absence of methanogens by coupling the oxidation of propionate to the reduction of sulfate. Growth on propionate plus sulfate was very slow (μ=0.024 day−1). An average growth yield was found of 1.5 g (dry weight) per mol of propionate. MPOB grew even slower than other sulfate-reducing syntrophic propionate-oxidizing bacteria. The growth rates and yields of strict sulfate-reducing bacteria (Desulfobulbus sp.) grown on propionate plus sulfate are considerably higher.
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    Agroforestry systems 30 (1995), S. 289-299 
    ISSN: 1572-9680
    Keywords: bunch weight ; blackpod ; underplanting ; growth ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Mature oil palms at the Oil Palm Research Institute at Kusi, Ghana were underplanted with cocoa to study the feasibility of mixed cropping of the two crops under West African conditions. The oil palm trees used in this trial were planted in 1970 and had achieved maximum canopy formation. Three triangular spacings of the oil palms used were 8.7, 9.9 and 10.5 m. The cocoa was underplanted in June 1988 at a spacing of 2.4 m triangular using mixed hybrids. There were no significant differences in oil palm yield between plots with cocoa and the controls. There was no appreciable damage to the cocoa trees from falling palm fronds and fruits during harvesting or pruning. Cocoa seedling growth and yield were significantly better under the oil palm spaced at 9.9 or 10.5 m triangular than under oil palm space at 8.7 m triangular.
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    Plant cell, tissue and organ culture 43 (1995), S. 111-116 
    ISSN: 1573-5044
    Keywords: anthraquinones ; auxins ; growth ; Morinda citrifolia ; secondary metabolite production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell suspensions ofMorinda citrifolia are able to produce large amounts of anthraquinones (AQ) when they are cultivated on a B5-medium containing 1 mg 1-1 naphtyl acetic acid (NAA); this production is inhibited by addition of 2,4-dichloro-phenoxyacetic acid (2,4-d). Also during cultivation on 1 mg 1-1 2,4-d AQ-production is absent. It appeared that in the presence of NAA a kind of ‘AQ-production’ program is switched on: cell division rate is low as well as metabolic activity, while endogenous sugar levels are high. The same properties develop in the presence of auxins like indolyl-acetic acid and p-chloro-phenylacetic acid. With 2,4-d and related auxins (like p-chloro-phenoxyacetic acid) AQ production is absent and emphasis is laid on a developmental program characterized by high cell division rates, high metabolic activity and low endogenous sugar contents. Independent of the type of auxin applied, the cells grow as a suspension consisting of finely dispersed cells. The ‘AQ-producing differentiation program’ cannot be maintained during a consecutive series of subculturings: with increasing AQ-contents the viability of the cells and the cell division rate decrease. The possible mechanisms of regulation of AQ-production by auxins are discussed as well as the advantages of the use of theMorinda model system in the study of the relation between growth, primary and secondary metabolism.
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  • 86
    ISSN: 1573-5060
    Keywords: bread-wheat ; chromosome deletion ; restriction fragment length polymorphism ; RFLP ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A study was undertaken to evaluate the breeding behaviour and to identify a spontaneously produced putative chromosomal deletion in the winter wheat (Triticum aestivum L. em. Thell.) cv Norstar. Male and female transmission studies of plants heterozygous for the deletion chromosome indicated 9.5% and 48.8% transmission through the pollen and the egg, respectively. Meiotic analyses of progeny from deletion heterozygotes indicated that the deletion chromosome was eliminated from half of the plants, which agreed with the male and female transmission frequencies. Testcrosses of the deletion chromosome with telocentrics and nullisomic-tetrasomic combinations suggested that the deletion involved the long arm of chromosome 5D. This was confirmed by restriction fragment length polymorphism (RFLP) analysis. Also, monosomic plants obtained in progeny of deletion heterozygotes were shown to be monosomic for 5D. Studies of plants homozygous for the deletion showed relatively normal pairing between the deletion chromosomes, and with the short arm (5DS), but not the long arm (5DL). Deletion homozygotes were self-sterile, and morphologically similar to plants nullisomic for 5D, but plants that also contained 5DL, or a homoeologous chromosome were self-fertile and had normal morphology. Studies of chromosome morphology indicated that the deletion chromosome was metacentric, and the length of the long arm was reduced by approximately 60%. RFLP studies showed that, in terms of genetic distance, 90% of the arm was missing.
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  • 87
    ISSN: 1573-5060
    Keywords: mitochondrial genome diversity ; perennial ryegrass ; Lolium perenne ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The extent of mitochondrial DNA restriction fragment length polymorphisms among individual plant samples of perennial ryegrass was determined. A total of 72 plants from three cultivars Yorktown II, S23 and Riikka were surveyed using three restriction enzymes (BamHI,EcoRI andHindIII) and three mitochondrial gene probes (coxI, coxIII andnad9). Polymorphisms were noted within each of the two cultivars Yorktown II and S23, whereas in ‘Riikka’ no variation was detected. It seems most likely that the mitochondrial genome diversity within the same cultivar has resulted from non-homogeneous ancestor cytoplasms. The hybridization-based assay employed is simple to perform, gives unambiguous results, and may thus be used in mass screening of perennial ryegrass populations for breeding purpose.
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  • 88
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    Plant growth regulation 16 (1995), S. 121-127 
    ISSN: 1573-5087
    Keywords: Brassica carinata ; growth ; paclobutrazol ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Foliar spraying of Brassica carinata (cv. PC 5) with paclobutrazol (PP 333) at 5, 10 and 20 μg ml-1 concentrations, reduced plant height significantly and modified the canopy structure by enhancing the number of branches (primary, secondary and tertiary) and the angle of insertion of primary branches on the main axis. The seed yield per plant also increased mainly due to increase in the number of siliquae per plant. Paclobutrazol increased total dry matter of plants and partitioning coefficients. The leaves in paclobutrazol-treated plants exhibited higher chlorophyll content, and they remained intact on plants for longer than the controls. The seeds from paclobutrazol-treated plants had higher levels of proteins, strarch and total soluble sugars but less total seed oil content.
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  • 89
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    Water, air & soil pollution 85 (1995), S. 1701-1706 
    ISSN: 1573-2932
    Keywords: nitrogen ; heathland ; Calluna vulgaris ; critical load ; growth ; litter production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Experimental additions of ammonium sulphate to a nitrogen-poor dry heathland have been carried out since 1989. There are four nitrogen treatments: a control (receiving artificial rain only), a low treatment which receives an additional 7.7 kg N ha−1 yr−1, a high treatment receiving 15.4 kg N ha−1 yr−1 and an alternating treatment which receives either the control or the high nitrogen additions, in alternate years. The estimated background deposition at the study site is 13–18 kg N ha−1 yr−1, a value similar to the critical load that has been suggested for the conversion of lowland heath to grassland. Over the past 5 years there have been significant stimulations in shoot growth, flowering, canopy density and litter production. Flowering, in particular, strongly reflects nitrogen additions in the alternating treatment. Current models of the response of dry Calluna heathlaud to enhanced nitrogen deposition suggest that higher tissue nitrogen levels will occur and will be accompanied by heightened sensitivity to secondary stresses. This may in turn lead to canopy breakdown and replacement by grassland. The application of nitrogen at deposition rates only slightly in excess of the critical load over five years has produced small, non-significant increases in shoot nitrogen content. However, there is clear evidence of a large positive effect on shoot growth, flowering, litter production and canopy density of Calluna. The observation of these responses at the application rates used in this study supports the current proposals for critical loads of nitrogen for lowland heaths.
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  • 90
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    Water, air & soil pollution 85 (1995), S. 1437-1442 
    ISSN: 1573-2932
    Keywords: ozone ; native flora ; premature senescence ; growth ; foliar symptoms ; seed production ; Malva sylvestris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This paper reports on open-top chamber studies investigating the effects of different O3 exposures on native herbaceous plant species. Plants were grown up from seeds, potted into natural soils and exposed to near-ambient O3 levels during one growing season. A wide range of visible symptoms was apparent during the exposures. Species such as Rumex obtusifolius, Senecio vulgaris or Sonchus asper showed leaf colorations (e.g. reddish pigmentation) that probably indicate a non-specific stress response. In other species especially of the genera Malva and Cirsium the symptoms produced by O3 appeared to be similar to those characteristic for O3-specific foliar injury (stippling, flecking). In almost all species tested, O3 caused premature leaf senescence, which was sometimes associated with premature leaf abscission. However, earlier senescence did not necessarily result in changes in plant growth. Of all species tested, Malva sylvestris was found to be the most sensitive in terms of growth reduction and lower seed production.
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  • 91
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    Ecotoxicology 4 (1995), S. 299-306 
    ISSN: 1573-3017
    Keywords: pH ; aluminium ; Triturus ; growth ; feeding behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: The distribution of smooth and palmate newts (Triturus vulgaris and Triturus helveticus) may be related to water quality. This study compared the larval growth and feeding behaviour of both species under sublethal levels of aluminium and low pH. The growth of both species was inhibited to a similar degree under acidic conditions with aluminium toxicity dependent upon both low pH and developmental stage. The two species were differentially affected by low pH in terms of feeding behaviour. T. vulgaris larvae took significantly longer to snap at food under low pH than the apparently acid-tolerant T. helveticus. Aluminium had no significant effect on the feeding behaviour of either species.
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  • 92
    ISSN: 1572-9788
    Keywords: marker-based selection ; nematode resistance ; potato ; PCR ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The use of RFLPs for marker-assisted selection schemes in potato breeding is hampered by the fact that RFLP technology requires good laboratory facilities, technical skills and high financial input. Marker technology based on the polymerase chain reaction (PCR) would facilitate the application of marker-assisted selection. PCR assays have been developed that are diagnostic for RFLP alleles at two marker loci,CP56 andCP113, which are closely linked in coupling to the nematode resistance allelesGro1 on chromosome VII andH1 on chromosome V of potato. By comparing DNA sequences among different marker alleles, point mutations were identified based on which allele-specific oligonucleotides were designed. Using allele-specific oligonucleotides as primers in PCR reactions, single-marker alleles were amplified by which the inheritance ofGro1 andH1 could be followed in crosses of diploid potato genotypes containing the genetically characterizedGro1 orH1 resistance allele. When tested in 136 unrelated tetraploid potato varieties, the marker allele indicative ofGro1 was not correlated with the presence of nematode resistance. The marker allele indicative for theH1 resistance allele was correlated with nematode resistance. It was, however, found in four varieties only of the 136 tested.
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  • 93
    ISSN: 1572-9788
    Keywords: disease resistance ; gene combination ; Oryza sativa L. ; RAPD ; RFLP ; Xanthomonas oryzae pv.oryzae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Four genes of rice,Oryza sativa L., conditioning resistance to the bacterial blight pathogenXanthomonas oryzae pv.oryzae (X. o. pv.oryzae), were tagged by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. No recombinants were observed betweenxa-5 and RFLP marker lociRZ390, RG556 orRG207 on chromosome 5.Xa-3 andXa-4 were linked to RFLP locusXNpb181 at the top of chromosome 11, at distances of 2.3 cM and 1.7 cM, respectively. The nearest marker toXa-10, also located on chromosome 11, was the RAPD locusO07 2000 at a distance of 5.3 cM. From this study, the conventional map [19, 28] and two RFLP linkage maps of chromosome 11 [14, 26] were partially integrated. Using the RFLP and RAPD markers linked to the resistance genes, we selected rice lines homozygous for pairs of resistance genes,Xa-4 +xa-5 andXa-4 +Xa-10. Lines carryingXa-4 +xa-5 andXa-4 +Xa-10 were evaluated for reaction to eight strains of the bacterial blight pathogen, representing eight pathotypes and three genetic lineages. As expected, the lines carrying pairs of genes were resistant to more of the isolates than their single-gene parental lines. Lines carryingXa-4 +xa-5 were more resistant to isolates of race 4 than were either of the parental lines (‘quantitative complementation’). No such effects were seen forXa-4 +Xa-10. Thus, combinations of resistance genes provide broader spectra of resistance through both ordinary gene action expected and quantitative complementation.
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  • 94
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    Molecular breeding 1 (1995), S. 389-395 
    ISSN: 1572-9788
    Keywords: barley ; Hordeum vulgare ; RFLP ; consensus map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A consensus linkage map of the barley genome was constructed. The map is based on six doubled haploid and one F2 population. The mapping data for three of the doubled haploid populations was obtained via the GrainGenes database. To allow merger of the maps, only RFLP markers that produce a single scorable band were included. Although this reduced the available markers by about half, the resultant map contains a total of 587 markers including 87 of known function. As expected, gene order was highly conserved between maps and all but two discrepancies were found in closely linked markers and are likely to result from the small population sizes used for some maps. The consensus map allows the rapid localisation of markers between published maps and should facilitate the selection of markers for high-density mapping in defined regions.
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  • 95
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    Water, air & soil pollution 85 (1995), S. 1343-1348 
    ISSN: 1573-2932
    Keywords: ozone episodes ; Fraxinus excelsior ; growth ; leaf area ; abscission ; open-top chambers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Ash trees were exposed to ozone episodes (150 nl l−1, 8 h d−1, 24–27 d y−1) from May to October, 1992 to 1994, in open-top chambers designed to minimise microclimatic modification. Many aspects of growth were studied, including leaf abscission, leaf area, mean leaf size, tree height and girth, extension growth, cross sectional area of annual rings and above-ground dry weight. An overview of the results is presented. This shows that the duration of the leaf fall period was increased by exposure to ozone. However, three seasons of severe ozone exposure relative to UK conditions did not significantly alter other aspects of growth.
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  • 96
    ISSN: 1573-8477
    Keywords: maintenance of sex ; unisexual reproduction ; frozen niche-variation model ; growth ; survival and reproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Clonal and sexual co-existence is common in a number of vertebrate taxa, even though the ‘cost of sex’ makes such co-existence theoretically unlikely. The frozen niche-variation (FNV) model explains this co-existence on the basis of differences in overall niche breadth and competition between clones and sexuals. In the present study I examined two predictions of the FNV model. First, I examined the prediction that genetically variable populations have higher relative fitness when compared with monoclonal populations by comparing the performances of clonal and outcrossed sexual strains ofPoeciliopsis in monocultures at two densities. The prediction of increased overall productivity for the sexuals was verified, with net reproductive rates for the sexuals being between two and four times as high as the clones. Second, I tested the prediction that derived clones will successfully compete with their sexual progenitor(s) in the narrow range to which the clones are adapted, while the sexuals should co-exist because of their ability to use a wider range of resources than any single clone. I examined this prediction by comparing performance variables (e.g. growth, fecundity and survival) of each strain in pure culture with their partitioned performance from the mixed treatments. Clonal performance increased in mixtures compared to monocultures, as expected. However, the expectation that the sexual's performance would be less affected by mixtures than the clones' performance, was not met. The sexuals had reduced growth and fecundity on a par with the increase in both variables in the clones. Therefore, support for the FNV model was mixed. Although the performance in monocultures suggests that the sexuals have a wider niche breadth than the clones, performances in mixtures do not indicate such a relationship. Switching of behaviours or resource-use patterns between mixed and pure cultures may have caused the equivocal results.
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