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  • RFLP
  • Springer  (66)
  • 1990-1994  (66)
  • 1991  (66)
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  • 1990-1994  (66)
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  • 1
    ISSN: 1420-9071
    Keywords: Mitochondrial DNA ; RFLP ; Leptinotarsa decemlineata ; Colorado potato beetle ; population genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This study demonstrates variability in restriction enzyme cleavage sites of mitochondrial DNA (mtDNA) among four popalations of Colorado potato beetle (CPB). A suite of three enzymes (EcoRI,HpaI,PstI) was sufficient to discriminate among the populations tested. Individuals heteroplasmic for restriction enzyme patterns were found in some populations. Variability in CPB mtDNA should prove useful in efforts to trace the origin and dispersal of the species in North America.
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  • 2
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    Entomologia experimentalis et applicata 61 (1991), S. 59-72 
    ISSN: 1570-7458
    Keywords: Consumption ; food utilization efficiencies ; growth ; Lycopersicon esculentum ; Manduca sexta ; Nicotiana tabacum ; potassium ; tobacco hornworm ; water regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Third instar tobacco hornworms (Manduca sexta L.: Sphingidae) on low dietary potassium had a lower relative growth rate than individuals on diets with potassium concentrations reflecting those in host-plants, due to decreased consumption rate, lower efficiencies of conversion of ingested and digested food (ECI and ECD), and a prolonged growth/feeding phase. Furthermore, these larvae, when placed on a diet with a moderate potassium concentration through the fourth stadium, ended up being smaller due to lower ECI and less biomass gained, and had a prolonged growth phase, which suggest an irreversible cost of the previous low potassium diet. Third instar hornworms on high potassium diets had lower ECI and ECD, and they had a prolonged growth phase. These individuals, when placed on a moderate potassium diet in the fourth stadium, gained less biomass, than those previously offered hostplant-like-potassium diets. Body potassium concentrations (% dw) at the end of the third stadium were similar among treatment groups. With increasing potassium concentrations in the diet, utilization efficiencies of potassium decreased and potassium concentrations in the frass increased. Correspondingly, water content (% fw) of the newly-molted fourth instar larvae declined with increasing potassium, indicating a passive loss of water during potassium excretion. Low and high dietary potassium reduced survivorship of third instar larvae; fourth instar caterpillars previously fed the low potassium diet also had poor survivorship. We conclude that, within the normal range of potassium concentrations in the hostplants, caterpillar performance is largely unaffected by potassium concentration, but that potassium-poor and potassium-rich diets, such as those hornworms may sometimes experience, can reduce growth and survivorship.
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  • 3
    ISSN: 1573-0832
    Keywords: Candida albicans ; ammonium ; physiology ; medium ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Candida albicans strain B 311-10 with and without starvation was cultivated in the minimal synthetic medium of Shepherd et al. [18], modified without biotin, aminoacids, low glucose concentration [20] and with decreasing amounts of (NH4)2SO4, to determine the optimal growth requirement for this strain. All the experiments were carried out under sterile conditions at 25 °C in a thermostat with initial O.D.s (675 nm) of 0.500 and 0.100. Cell growth was generally monitored everyday for six days with a spectrophotometer by determining the absorbance of the cultures at 675 nm. All the experiments were repeated three times and a statistical analysis of the data with a probability of 99% and 1% of error was performed to confirm the validity of the results. Best growth was obtained with starved cells at an initial O.D. of 0.100 and with a 0.1 g/L concentration of (NH4)2SO4. At this concentration, the growth of C. albicans B 311-10 was best between the first and the fourth day with the maximum at the third day. With (NH4)2SO4 concentrations of 0.05 and 0.5 g/L, cell growth was the same.
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  • 4
    ISSN: 1432-203X
    Keywords: wheat ; rye ; embryogenesis ; growth ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of the short arm of rye chromosome 1 (1RS) from Secale cereale var. Imperial on the growth and differentiation of callus cultures from wheat Triticum aestivum var. Chinese Spring immature embryos was analysed. This chromosome arm was found to stimulate both embryogenesis and the rate of growth of calli. Recombinant lines carrying segments of 1RS were used to delineate the regions of 1RS responsible for the tissue culture effects. The enhancement of embryogenesis and the stimulation of growth were shown to be associated with two distinct genetic regions of the chromosome arm; the former is located between the centromere and the Sec 1 locus, while the latter is situated in the immediate vicinity of the Sec 1 locus.
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  • 5
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    Plasma chemistry and plasma processing 11 (1991), S. 371-386 
    ISSN: 1572-8986
    Keywords: Plasmas ; aerosol ; modelling ; growth ; reactor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract A model is proposed for the analysis of the production of ultrafine particles in thermal plasma reactors. The model initially solves the fluid flow, temperature, and concentration fields using a classical control volume approach. The nucleation and growth of ultra fine particles are then solved along each streamline. The evolution of the particle distribution is described by a statistical approach, using the first moments of the distribution as the dependent variables. Brownian coalescence is considered in the free molecular regime. In the discussion, the model is used to demonstrate the effects of some important parameters, such as the initial concentration of metal vapor, its radial distribution, and the radial injection of a cooling gas, on the particle size distribution.
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  • 6
    ISSN: 1573-5028
    Keywords: cell wall proteins ; growth ; roots ; stems ; water deficit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined growth, water status and gene expression in dark-grown soybean (Glycine max L. Merr.) seedlings in response to water deficit (low water potentials) during the first days following germination. The genes encoded the plasma membrane proton ATPase and two proteins of 28 kDa and 31 kDa putatively involved in vegetative storage. Water potentials of stems and roots decreased when 2-day-old seedlings were transferred to water-saturated air. Stem growth was inhibited immediately. Root growth continued at control rates for one day and then was totally inhibited when the normal root-stem water potential gradient was reversed. Expression of mRNA for the 28 kDa and 31 kDa proteins, measured independently using specific 3′-end probes, occurred about equally in stems. However, only the mRNA for the 31 kDa protein was detected in roots and at a lower abundance than in stems. Low water potentials increased the mRNA only for the 28 kDa protein in stems and the 31 kDa protein in roots. This differential expression followed the inhibition of stem growth but preceded the inhibition of root growth. The expression of the message for the ATPase, measured using a probe synthesized from a partial oat ATPase clone, was low in stems and roots but there was a 6-fold increase at low water potentials in roots. The increase followed the inhibition of root growth. This appears to be the first instance of regulation of ATPase gene expression in plants and the first demonstration of differential expression of the 28 kDa, 31 kDa, and ATPase messages. The correlation with the differential growth responses of the stems and roots raises the possibility that the differential gene expression could be involved in the growth response to low water potentials.
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  • 7
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    Plant molecular biology 16 (1991), S. 831-839 
    ISSN: 1573-5028
    Keywords: RFLP ; Oryza ; rice ; genome evolution ; allotetraploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment length polymorphisms (RFLPs) were studied in fourteen accessions of CCDD genome allotetraploid wild rice species (Oryza latifolia, O. alta and O. grandiglumis). Fourteen nuclear RFLP markers previously mapped in AA genome-cultivated rice were used as probes. A phylogenetic tree, constructed by parsimony analysis based on RFLPs, grouped the accessions according to their geographic origin from Central or South America. Oryza alta, O. grandiglumis and one accession of O. latifolia grouped together as a subgroup, and our results suggested that the three taxa should be considered as populations of a single complex species. Duplicate loci, representing the two constituent genomes of the allotetraploid, were observed for most RFLP markers. By comparing RFLPs from the allotetraploids with those from a CC genome diploid wild species (O. officinalis), it was possible to detect RFLPs specific for both the CC and DD genomes of the allotetraploid. In inter-accession F2 populations, independent segregation of RFLP markers for CC and DD genomes was observed.
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  • 8
    ISSN: 1573-5028
    Keywords: chromosome assignment ; dihaploid progenies ; heterozygosity ; potato ; rDNA ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction map and organization of rDNA was inferred from analysis of dihaploid progenies of two tetraploid genotypes of cultivated potato. Each tetraploid genotype was characterized by a specific distribution of different types of rDNA repetition units on their four homologous chromosomesII. The genotypes were heterozygous and differed by the kind of units carried by each chromosomeII. Models for the generation of the observed organization are discussed and supported by first cloning studies.
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  • 9
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    Theoretical and applied genetics 82 (1991), S. 393-398 
    ISSN: 1432-2242
    Keywords: Maize ; Helminthosporium turcicum race1 ; RFLP ; NILs ; Mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have identified tight linkage of an RFLP marker to theHt1 gene of maize that confers resistance to the fungal pathogenHelminthosporium turcicum race 1. This was accomplished by the use of four pairs of near isogenic lines (NILs; B73, A619, W153R, and CM105), each differing by the presence or the absence of the geneHt1. SinceHt1 maps to chromosome 2, 26 clones already mapped to this chromosome were labeled and probed against Southern blots of these NILs DNA digested with three restriction enzymes:EcoRI,BamHI, andHindIII. Six markers exhibited an RFLP for at least one pair of NILs. Presumptive linkage was further tested by analyzing the segregation of five of the six markers (one was monomorphic in the cross studied) and resistance toH. turcicum race 1 on 95 F2 individuals from the cross DF20 × LH146Ht. The results indicate a tight linkage between one of the DNA markers,UMC150B, and theHt1 gene.
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  • 10
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    Theoretical and applied genetics 82 (1991), S. 697-703 
    ISSN: 1432-2242
    Keywords: RFLP ; Bamboo ; Phyllostachys ; Chloroplast DNA ; Germplasm screening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Bamboo species are difficult to identify because flowering material is seldom available and taxonomy is of necessity based on vegetative characters. To evaluate the utility of restriction fragment length polymorphism (RFLP) analysis in bamboo systematics and germplasm screening, a library of random genomic probes from a Phyllostachys nigra PstI library was constructed. Probes from the library were used to screen bamboo germplasm consisting mostly of temperate bamboos of the genus Phyllostachys. RFLP variation was abundant, and species-specific patterns were readily obtained. Chloroplast DNA showed little variation among the bamboo accessions analyzed.
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  • 11
    ISSN: 1432-2242
    Keywords: RFLP ; PCR ; Rice ; Inheritance ; Nonradioactive
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thirty mapped Indica rice genomic (RG) clones were partially sequenced from each end. From such sequence data, pairs of oligonucleotides were synthesized to act as primers for polymerase chain reaction (PCR) amplification of the corresponding loci in crude total DNA preparations. The PCR products from DNA of Indica varieties were of the sizes expected from the sizes of the corresponding RG clones. However, size polymorphisms were seen between PCR products from Indica and Japonica varieties, and among wildOryza species. Restriction fragment length polymorphism (RFLP) was observed between PCR products of Indica varieties simply by electrophoretic analysis of restricted products, without the need for Southern hybridization or radiolabelling. The RFLPs noted between varieties ARC6650 and Phalguna were inherited in recombinant inbred lines derived from a cross between them. The RFLPs were detectable in PCR products amplified from DNA extracted by a simple procedure from single seedlings or leaves, and revealed genetic heterogeneity in cultivated lines. An approach is described that is relevant to the acceleration of classical plant breeding through molecular techniques.
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  • 12
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    Theoretical and applied genetics 83 (1991), S. 89-96 
    ISSN: 1432-2242
    Keywords: RFLP ; Alfalfa ; Genetic diversity ; Phylogenetic tree ; Gene duplication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Alfalfa (Medicago sativa L.) is a major forage crop throughout the world. Although alfalfa has many desirable traits, continued breeding is required to incorporate pest resistances and other traits. We conducted this study to determine the amount of restriction fragment length polymorphism (RFLP) variability present within and between diploid and tetraploid alfalfa populations, and whether or not this variability is sufficient for construction of an RFLP map. Diploid plants from M. sativa ssp. falcata, ssp. coerulea, and ssp. sativa and tetraploid spp. sativa cultivars ‘Apollo,’ ‘Florida 77,’ and ‘Spredor 2’ were included. A total of 19 cDNA clones was probed onto genomic Southern blots containing DNA digested by EcoRI, HindIII, or BamHI. Phylogenetic trees were produced, based on parsimony analysis of shared restriction fragments. Evidence for extensive gene duplication was found; most probes detected complex patterns of restriction fragments. Large amounts of variation are present within all diploid subspecies. M. sativa ssp. falcata plants formed clusters distinct from ssp. sativa or ssp. coerulea plants, which were not distinctly clustered. Some M. sativa ssp. falcata plants were more similar to the other groups than to other plants within ssp. falcata. Variation among tetraploid cultivars showed that Florida 77 and Apollo had more similarities than either showed with Spredor 2. All three cultivars showed large within-population variation, with Apollo being the most diverse and Spredor 2 the least. Based on these results, development of an RFLP map at the diploid level appears possible. Also, differentiation of cultivars, particularly ones of divergent origin, seems possible based on RFLP patterns.
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  • 13
    ISSN: 1432-2242
    Keywords: Lycopersicon esculentum ; L. pennellii ; Fusarium wilt ; RFLP ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inheritance and linkage relationships of a gene for resistance to Fusarium oxysporum f. sp. lycopersici race 1 were analyzed. An interspecific hybrid between a resistant Lycopersicon pennellii and a susceptible L. esculentum was backcrossed to L. esculentum. The genotype of each backcross-1 (BC1) plant with respect to its Fusarium response was determined by means of backcross-2 progeny tests. Resistance was controlled by a single dominant gene, I1, which was not allelic to I, the traditional gene for resistance against the same fungal pathogen that was derived from L. pimpinellifolium. Linkage analysis of 154 molecular markers that segregated in the BC1 population placed I1 between the RFLP markers TG20 and TG128 on chromosome 7. The flanking markers were used to verify the assignment of the I1 genotype in the segregating population. The results are discussed with reference to the possibility of cloning Fusarium resistance genes in tomato.
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  • 14
    ISSN: 1432-2242
    Keywords: Plasmid-like DNA ; Rice ; Mitochondrial DNA ; RFLP ; Nucleo-mitochondrial DNA transmission
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary B1 and B2 are small, circular, mitochondrial plasmid-like DNAs found in male-sterile cytoplasm (cms-Bo) of rice. In this study, nuclear sequences homologous to these DNAs were investigated among a number of rice cultivars. Several copies of nuclear B1-and B2-homologous sequences were detected in all examined cultivars, regardless of the presence or absence of the B1 and B2 DNAs in mitochondria, indicating that the existence of the B1- and B2-homologous sequences in the rice nuclear genome was widespread. A restriction fragment length polymorphism (RFLP) was detected for both sequences, and we propose that these DNAs could be useful RFLP markers for the rice nuclear genome. To analyze these nuclear homologues genetically, segregation analysis of the RFLP was carried out in the F2 progenies of an Indica-Japonica rice hybrid. Of the B1 homologues, there were two nonallelic fragments, one specific to the Indica parent and the other to the Japonica. These results indicate that the B1 and B2 homologues were dispersed in the nuclear genome. The integration of B1-homologous DNA into the nuclear DNA may have occurred independently after sexual isolation of the Indica and Japonica rice varietal groups, or a intranuclear transposition of these sequences took place during the process of rice differentiation into the varietal groups.
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  • 15
    ISSN: 1432-2242
    Keywords: Phaseolus interspecific hybrid ; RFLP ; Beans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphism (RFLP) was determined among P. vulgaris genotypes and Phaseolus species using 19 probes. The incidence of polymorphism was high (70–86%) between species, but relatively low (22–26%) between genotypes of P. vulgaris. Suitable probes were identified for the analysis of P. vulgaris and P. coccineus hybrids. The segregation pattern in F2 populations was Mendelian for two probes (LHB and VEE20) and non-Mendelian for GS-g, CHS, and CHI. Statistical analyses indicated gametic selection with preferential transmission of the P. vulgaris alleles, which may account for the selective recovery of P. vulgaris progeny types observed earlier. The available hybrids of P. vulgaris and P. coccineus and the high degree of interspecific RFLP will facilitate the construction of a linkage map for Phaseolus.
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  • 16
    ISSN: 1432-2242
    Keywords: Rye ; Genetic mapping ; RFLP ; Storage protein ; Isozyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A map of chromosome 1R of rye was constructed using 16 molecular and biochemical loci. From long arm to short arm, known-function loci were placed in the order: XAdh — XLee — Glu-R1[Sec-3] — XPpdk-1R — XEm-1R-1 — XEm-1R-2 — Centromere — XNor-R1 —Gpi-R1 — XGli-R1 [Sec-1a] along with six anonymous genomic and cDNA clones from wheat. The map, which spans 106 cM with 12 loci clustered in a 15-cM region around the centromere, shows reasonably good agreement with previously published maps for the centromeric region, whereas the XNor-R1 — Gpi-R1 region gives a much larger distance than previously reported.
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  • 17
    ISSN: 1432-2242
    Keywords: RFLP ; Potato ; Tomato ; Genetic maps
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An RFLP linkage map of the potato is presented which comprises 304 loci derived from 230 DNA probes and one morphological marker (tuber skin color). The self-incompatibility locus of potato was mapped to chromosome I, which is homoeologous to tomato chromosome I. By mapping chromosome-specific tomato RFLP markers in potato and, vice versa, potato markers in tomato, the different potato and tomato RFLP maps were aligned to each other and the similarity of the potato and tomato genome was confirmed. The numbers given to the 12 potato chromosomes are now in accordance with the established tomato nomenclature. Comparisons between potato RFLP maps derived from different genetic backgrounds revealed conservation of marker order but differences in chromosome and total map length. In particular, significant reduction of map length was observed in interspecific compared to intraspecific crosses. The distribution of regions with distorted segregation ratios in the genome was analyzed for four potato parents. The most prominent distortion of recombination was found to be caused by the self-incompatibility locus.
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  • 18
    ISSN: 1432-2242
    Keywords: Brassica ; Genome ; Isozymes ; RFLP ; Fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Six Brassica napus — B. nigra disomic addition lines were characterized by isozyme, fatty acid, and RFLP markers. The markers were arranged in six synteny groups, representing six of the eight chromosomes present in the B. nigra genome. Synteny group 1 displayed high levels of linoleic and linolenic acids in the seeds of the B. nigra parent. Synteny group 3 accumulated higher levels of eicosenoic and erucic acid than B. nigra. Three of the lines transmitted the alien chromosome to 100% of the progeny. The rest had variable transmission rates but all were above 50%. Most of the lines produced disomic addition plants in their progeny, suggesting pollen transmission of the alien chromosome. In addition to the marked lines, six others remained unmarked. These could be grouped into two classes according to their alien chromosome transmission. It is likely that they represent the two other B. nigra chromosomes that remained uncharacterized by the markers. No diploid individuals carrying B. nigra genome-specific markers were detected in the progenies studied.
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  • 19
    ISSN: 1432-2242
    Keywords: Glycine max ; Near-isogenic lines ; Molecular markers ; RFLP ; Linkage ; Genetic map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A molecular marker analysis of a near-isogenic line (NIL), its donor parent (DP), and its recurrent parent (RP) can provide information about linkages between molecular markers and a conventional marker introgressed into the NIL. If the DP and RP possess different alleles for a given molecular marker, and if the NIL possesses the same allele as the DP, then it is reasonable to presume a linkage between that molecular marker and the introgressed marker. In this study, we examined the utility of RFLPs as molecular markers for the NIL genemapping approach. The allelic status of fifteen RFLP loci was determined in 116 soybean RP/NIL/DP line sets; 66 of the ‘Clark’ RP type and 50 of the ‘Harosoy’ RP type. Of the 1740 possible allelic comparisons (116 NILs x 15 RFLP loci), 1638 were tested and 462 (33.9%) of those were informative (i.e., the RP and DP had different RFLP alleles). In 15 (3.2%) of these 462 cases the NIL possessed the DP-derived RFLP allele, leading to a presumption of linkage between the RFLP locus and the introgressed conventional marker locus. Two presumptive linkages, pK-3 — and pK-472 — Lf i, were subsequently confirmed by cosegregation linkage analysis. Although not yet confirmed, two other associations, pk-7 ab and pK-229 — y 9 seemed to be plausible linkages, primarily because the pk-7 — ab association was detected in two independently derived NILs and both markers of the pK-229 — y 9 association were known to be linked to Pb. The data obtained in this investigation indicated that RFLP loci were useful molecular markers for the NIL gene-mapping technique.
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  • 20
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    Theoretical and applied genetics 81 (1991), S. 221-226 
    ISSN: 1432-2242
    Keywords: RFLP ; Deletion mapping ; Genome evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A series of RFLP and isozyme markers were followed in the progenies of two alien addition lines of Brassica campestris-oleracea. One of the lines, carrying the C genome chromosome 4 as the alien chromosome, was surveyed for six markers. Fifty-four percent of the plants carrying alien chromosomes displayed all the expected makers, whereas the rest had one to five markers missing. The second line for C genome chromosome 5 displayed a similar behavior when surveyed for three markers. All three markers were transmitted together in 46% of the plants carrying alien chromosomes, whereas the rest carried only one or two of the markers. The loss of markers was associated with reduced chromosome size caused by deletions. The observed chromosome deficiencies permitted deletion analysis for a rough physical mapping and ordering of the markers on the two C genome chromosomes. The deletions observed may represent another mechanism for molding the chromosomes of the Brassica genomes during their evolution.
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  • 21
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    Theoretical and applied genetics 81 (1991), S. 227-232 
    ISSN: 1432-2242
    Keywords: Callus cultures ; RFLP ; Zea mays ; Oryza sativa ; Regeneration ; Somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tissue culture of the Zea mays inbred line A188 resulted in the regeneration of plants having a high level of phenotypic variation compared to seed-grown control plants. To determine how such variation was induced and whether this could be related to specific in vitro culture methods, callus cultures were established and maintained on different, commonly used culture media. Plants were regenerated and the genomic DNA of callus cultures and regenerants analysed for RFLP differences. The results show that regardless of the gene probe used, callus formation resulted in significant deviations from the DNA pattern normally found in seed-grown control plants. Alterations in gene copy number also occurred. As differentiation and organogenesis began, the level of DNA variation fell, and most of the regenerated plants showed a genetic similarity to the controls; those with RFLP differences were the somaclonal variants.
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  • 22
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    Theoretical and applied genetics 81 (1991), S. 397-400 
    ISSN: 1432-2242
    Keywords: Hexaploid wheat ; Chromosome-specific ; DNA sequence ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An unusual genomic DNA clone, PSR454, was isolated from a partial genomic library of wheat. This sequence is moderately repeated and detects at least 30 related sequences, all located in a tight linkage block on the long arm of chromosome 3B. When used as a RFLP probe, PSR454 detects a high level of polymorphism between wheat varieties that carry the sequence. There is no detectable hybridisation to sequences in one-third of the varieties tested, providing an “on-off” polymorphism that can be detected on dot blots, rather than the more resource-consuming conventional Southern analysis.
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  • 23
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    Theoretical and applied genetics 81 (1991), S. 565-570 
    ISSN: 1432-2242
    Keywords: Peanut ; RFLP ; Plant breeding ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary RFLP variability was studied in eight U.S. peanut cultivars, representing the four market types, and in 14 wild Arachis species accessions, using random genomic clones from a PstI library. Very low levels of RFLP variability were found among the allotetraploids, which included the U.S. cultivars and Arachis monticola, a wild species. The diploid wild species were very diverse, however. RFLP patterns of the allotetraploids were more complex than the diploids, and the two constituent genomes could usually be distinguished. On the basis of RFLP band sharing, A. ipaensis, A. duranensis, and A. spegazzinii appeared most closely related to the diploid progenitor species of the allotetraploids. A dendrogram of relationships among the diploid wild species was constructed based on band sharing.
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  • 24
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    Theoretical and applied genetics 82 (1991), S. 57-64 
    ISSN: 1432-2242
    Keywords: Cultivated rice ; Wild rice ; RFLP ; Satellite DNA ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 352-bp EcoRI fragment from rice DNA was cloned and shown to be a member of a tandem repeat. Sequence determination revealed homologies with human alpha satellite DNA and maize knob heterochromatin specific repeat. This 352-bp sequence is highly specific for the AA genome of rice. However, copy number and sequence organization are variable, depending on the accession analyzed. Several examples of amplification were observed in O. rufipogon and O. longistaminata. Use of resolutive polyacrylamide gel electrophoresis and 4-bp cutter enzymes allowed one to distinguish between the Indica and Japonica subtypes of O. sativa. The same method also discriminates between two groups of O. rufipogon, the presumed ancestor of O. sativa, suggesting that the present day Indica and Japonica subtypes originated independently from two O. rufipogon distinct populations.
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  • 25
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    Theoretical and applied genetics 82 (1991), S. 771-776 
    ISSN: 1432-2242
    Keywords: Secale cereale ; RFLP ; α-Amylase ; Genetics ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rye α-Amy1, α-Amy2, and α-Amy3 genes were studied in the cross between inbred lines using wheat α-amylase cDNA probes. The α-Amy1 and α-Amy2 probes uncovered considerable restriction fragment length polymorphism, whereas the α-Amy3 region was much more conserved. The numbers of restriction fragments found and the F2 segregation data suggest that there are three α-Amy1 genes, two or three α-Amy2 genes, and three α-Amy3 genes in rye. These conclusions were supported by a simultaneous study of α-amylase isozyme polymorphism. The F2 data showed the three individual α-Amy1 genes to span a distance of 3cM at the locus on chromosome 6RL. The genes were mapped relative to other RFLP markers on 6RL. On chromosome 7RL two α-Amy2 genes were shown to be separated by 5 cM. Linkage data within α-Amy3 on 5RL were not obtained since RFLP could be detected at only one of the genes.
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  • 26
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    Theoretical and applied genetics 83 (1991), S. 173-178 
    ISSN: 1432-2242
    Keywords: Lycopersicon ; Sex ; Crossing-over ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have determined that meiotic recombination differs between male and female gametes derived from the same plant. A single F1 plant was backcrossed to each of the parents, Lycopersicon esculentum and L pennellii, as the male (BCE) and female (BCP) parent, respectively. A total of 85 RFLP markers, covering more than 75% of the tomato genome, was used to construct a genetic map for both populations. Since both recurrent parents were homozygous, recombination measured in each population reflects crossing-over rates leading to male (BCE) and female (BCP) gametes. Comparisons were made by interval (genetic distance between two adjacent markers), by chromosome, and for the total length of the genome. Significantly less recombination was observed for male gametes at all levels. No significant relationship was found between areas of reduced recombination and approximate location to the centromere. That selection plays some role could not be eliminated, but no clear evidence was observed for single-locus selection as a major factor in the general reduction of crossing-overs in male gametes.
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  • 27
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    Theoretical and applied genetics 83 (1991), S. 209-216 
    ISSN: 1432-2242
    Keywords: Wheat ; Gli-1 loci ; Gliadins ; LMW glutenins ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Probes related to γ-gliadins and to the LMW subunits of glutenin were used to determine the complexity of the Gli-1 loci, by RFLP analysis of euploid and aneuploid lines of bread wheat cv Chinese Spring and durum wheat cv Langdon. The two probes hybridised to separate sets of fragments derived from chromosomes 1 A, 1 B and 1D. The fragments related to the LMW subunit probe had a total copy number in HindIII digests of about 35 in Chinese Spring and 17 in Langdon, with more fragments derived from chromosomes 1D. The fragments hybridising to the γ-gliadin probe could be divided into two classes, based on whether they hybridised to the whole probe at high stringency or to the 3′ nonrepetitive region at moderate stringency. The fragments that failed to hybridise under these conditions were considered to be related to ω-gliadins. The fragments related to γ — and co-gliadins had total copy numbers of about 39 and 16, respectively, in HindIII digests of Chinese Spring, and about 24 and 12, respectively, in Langdon.
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  • 28
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    Journal of applied phycology 3 (1991), S. 61-82 
    ISSN: 1573-5176
    Keywords: Furcellaria ; morphology ; life history ; chemical constituents ; ecophysiology ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Information on the commercially utilized marine red alga Furcellaria lumbricalis is summarized from published sources. Biological aspects under discussion include: nomenclature, taxonomy, morphology, development and life history, distribution, phenology, ecophysiology, growth and resource management. A brief history of the utilization of this species, and an account of its chemical constituents including the hydrocolloid furcellaran are also presented.
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  • 29
    ISSN: 1573-5176
    Keywords: CO2 ; growth ; pH ; photosynthesis ; Porphyra yezoensis ; red alga
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    Topics: Biology
    Notes: Abstract Leafy thalli of the red algaPorphyra yezoensis Ueda, initiated from conchospores released from free-living conchocelis, were cultured using aeration with high CO2. It was found that the higher the CO2 concentration, the faster the growth of the thalli. Aeration with elevated CO2 lowered pH in dark, but raised pH remarkably in light with the thalli, because the photosynthetic conversion of HCO 3 − to OH− and CO2 proceeded much faster than the dissociation of hydrated CO2 releasing H+. Photosynthesis of the alga was found to be enhanced in the seawater of elevated dissolved inorganic carbon (DIC, CO2 + HCO 3 − + CO 3 − ). It is concluded that the increased pH in the light resulted in the increase of DIC in the culture media, thus enhancing photosynthesis and growth. The relevance of the results to removal of atmospheric CO2 by marine algae is discussed.
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  • 30
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    Journal of applied phycology 3 (1991), S. 319-327 
    ISSN: 1573-5176
    Keywords: Dunaliella viridis ; growth ; salinity ; temperature ; nitrogen concentration ; batch culture
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    Topics: Biology
    Notes: Abstract The growth of a strain ofD. viridis has been studied in batch culture under different combinations of temperature, salinity and nitrogen concentrations. Changes in these variables have a significant effect on cell division, biomass production, cell volume and pigment yield. This strain grows optimally at 1 M NaCl and 30 °C. Increasing salinity up to 4 M NaCl leads to a significant decrease of cell division rate and maximal population; growth at lower temperature decreases the rate of division of the cells but increases maximal cell density. Pigment yield decreases with increasing salinity and increases with increasing temperature. Nitrogen concentration has a large effect on total cell biomass and pigment production, but not on cell division rate. Saturation of growth occurs at 5 mM NO 3 − ; higher concentration (e.g. 10 mM) leads to a decrease of maximal cell density and photosynthetic pigment content.
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  • 31
    ISSN: 1573-5176
    Keywords: fluorescence ; growth ; pigments ; phytoplankton ; population dynamics
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    Notes: Abstract In applied water ecology several methods for estimating the biomass or activity of phytoplankton depend on the proportion of accessory pigments (xanthophylls) to chlorophyll a. Therefore, changes in pigmentation during growth and stationary phase were investigated in four different species (Amphidinium klebsii, Euglena gracilis, Prymnesium parvum, Cryptomonas ovata) typical representatives of the major algal groups. The ratios of the different xanthophylls to chlorophyll a depended not only on the growth phase, but also on the species. InAmphidinium andEuglena, the ratio of xanthophylls to chlorophyll rises continuously during the growth phase and declined during the stationary phase. InPrymnesium, quantitative pigmentation was found to be nearly independent of the growth phase. InCryptomonas, however, this ratio was relatively constant during growth, but increased in the stationary phase. In contrast to higher plants, in which the breakdown of chlorophylls occurs before that of the xanthophylls, in three of the species both pigment classes were reduced in parallel when the cultures were in the stationary phase. AgingCryptomonas, however, exhibited a pigment breakdown pattern similar to higher plants. The use of these findings for the widely applied biomass determination by chlorophyll fluorescence and for other pigment-based methods is discussed.
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  • 32
    ISSN: 1573-5176
    Keywords: fluorescence ; growth ; pigments ; phytoplankton ; population dynamics
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    Notes: Abstract In applied water ecology several methods for estimating the biomass or activity of phytoplankton depend on the proportion of accessory pigments (xanthophylls) to chlorophyll a. Therefore, changes in pigmentation during growth and stationary phase were investigated in four different species (Amphidinium klebsii, Euglena gracilis, Prymnesium parvum, Cryptomonas ovata) typical representatives of the major algal groups. The ratios of the different xanthophylls to chlorophyll a depended not only on the growth phase, but also on the species. InAmphidinium andEuglena, the ratio of xanthophylls to chlorophyll rises continuously during the growth phase and declined during the stationary phase. InPrymnesium, quantitative pigmentation was found to be nearly independent of the growth phase. InCryptomonas, however, this ratio was relatively constant during growth, but increased in the stationary phase. In contrast to higher plants, in which the breakdown of chlorophylls occurs before that of the xanthophylls, in three of the species both pigment classes were reduced in parallel when the cultures were in the stationary phase. AgingCryptomonas, however, exhibited a pigment breakdown pattern similar to higher plants. The use of these findings for the widely applied biomass determination by chlorophyll fluorescence and for other pigment-based methods is discussed.
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  • 33
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    International journal of anthropology 6 (1991), S. 257-265 
    ISSN: 1824-3096
    Keywords: Children somatotyping ; Spanish population ; growth
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    Topics: Biology
    Notes: Abstract Using the Heath-Carter (1972) method modified byHebbelinck et al. (1973), the somatotypes ina Spanish school-age population were determined. The study shows an evolution in the components of the somatotype with age, and a distribution of somatoplots in the somatochart which presnts peculiar characteristics in both sexes.
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  • 34
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    Journal of applied phycology 3 (1991), S. 319-327 
    ISSN: 1573-5176
    Keywords: Dunaliella viridis ; growth ; salinity ; temperature ; nitrogen concentration ; batch culture
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    Topics: Biology
    Notes: Abstract The growth of a strain ofD. viridis has been studied in batch culture under different combinations of temperature, salinity and nitrogen concentrations. Changes in these variables have a significant effect on cell division, biomass production, cell volume and pigment yield. This strain grows optimally at 1 M NaCl and 30 °C. Increasing salinity up to 4 M NaCl leads to a significant decrease of cell division rate and maximal population; growth at lower temperature decreases the rate of division of the cells but increases maximal cell density. Pigment yield decreases with increasing salinity and increases with increasing temperature. Nitrogen concentration has a large effect on total cell biomass and pigment production, but not on cell division rate. Saturation of growth occurs at 5 mM NO 3 − ; higher concentration (e.g. 10 mM) leads to a decrease of maximal cell density and photosynthetic pigment content.
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  • 35
    ISSN: 1573-5176
    Keywords: CO2 ; growth ; pH ; photosynthesis ; Porphyra yezoensis ; red alga
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Leafy thalli of the red algaPorphyra yezoensis Ueda, initiated from conchospores released from free-living conchocelis, were cultured using aeration with high CO2. It was found that the higher the CO2 concentration, the faster the growth of the thalli. Aeration with elevated CO2 lowered pH in dark, but raised pH remarkably in light with the thalli, because the photosynthetic conversion of HCO 3 − to OH− and CO2 proceeded much faster than the dissociation of hydrated CO2 releasing H+. Photosynthesis of the alga was found to be enhanced in the seawater of elevated dissolved inorganic carbon (DIC, CO2 + HCO 3 − + CO 3 − ). It is concluded that the increased pH in the light resulted in the increase of DIC in the culture media, thus enhancing photosynthesis and growth. The relevance of the results to removal of atmospheric CO2 by marine algae is discussed.
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  • 36
    ISSN: 1573-5176
    Keywords: Botryococcus braunii ; exopolysaccharide biosynthesis ; temperature profiles ; growth
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    Topics: Biology
    Notes: Abstract Temperature profiles (range 20–33 °C) were obtained for growth and exopolysaccharide (EPS) biosynthesis of the microalga Botryococcus braunii strain UC 58 under photoautotrophic conditions. The maximum temperature for growth was 32 °C and the temperature dependence of the specific growth rate was described by the Hinshelwood equation based on the Arrhenius relationship. The optimal range of temperatures for growth and extracellular EPS synthesis (25–30 °C) concurred and production of 4.5–5 g l−1 of EPS was obtained routinely, leading to high broth viscosities. Below 23 °C EPS biosynthesis was negligible, although the specific growth rate maintained high values. At supraoptimal temperatures EPS biosynthesis decreased, accompanying the increase in doubling time. The polymers formed at temperatures within the optimal range for production, when dissolved in water, produced solutions (2 gl−1) with the highest viscosity, suggesting that their molecular weight showed the highest values. The degree of polymerization of the EPS synthesized at suboptimal and supraoptimal temperatures was significantly below the values within the optimal range.
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  • 37
    ISSN: 1617-4623
    Keywords: Potato virus X ; Resistance genes ; RFLP ; Solanum tuberosum ; Genetic introgression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two different chromosomal locations of major genes controlling extreme resistance to potato virus X (PVX) were found by restriction fragment length polymorphism (RFLP) analysis of two populations segregating for the resistance. The resistance geneRx1 mapped to the distal end of chromosome XII, whereasRx2 was located at an intermediate position on linkage group V in a region where reduced recombination and segregation distortion have also been observed. These linkage anomalies were due to abnormal behaviour of the chromosome contributed by the resistant parent P34. The results presented were obtained using two different strategies for mapping genes of unknown location. One approach was the use of probes revealing polymorphic loci spread throughout the genome and resulted in the mapping ofRx1. The second approach was based on the assumption of possible linkage between the resistance gene and clone-specific DNA fragments introduced from a wild potato species.Rx2 was mapped by adopting this strategy.
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  • 38
    ISSN: 1617-4623
    Keywords: Soybean ; Symbiosis ; Nitrogen fixation ; RFLP ; Plant genome
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    Topics: Biology
    Notes: Summary The genetic locus (nts) controlling nitrate-tolerant nodulation, supernodulation, and diminished autoregulation of nodulation of soybean (Glycine max (L.) Merill) was mapped tightly to the pA-132 molecular marker using a restriction fragment length polymorphism (RFLP) detected by subclone pUTG-132a. The nts (nitrate-tolerant symbiotic) locus of soybean was previously detected after its inactivation by chemical mutagenesis. Mutant plant lines were characterized by abundant nodulation (supernodulation) and tolerance to the inhibitory effects of nitrate on nodule cell proliferation and nitrogen fixation. The large number of RFLPs between G. max line nts382 (homozygous for the recessive nts allele) and the more primitive soybean G. soja (P1468.397) allowed the detection of co-segregation of several diagnostic markers with the supernodulation locus in F2 families. We located the nts locus on the tentative RFLP linkage group E about 10 cM distal to pA-36 and directly next to marker pA-132. This very close linkage of the molecular marker and the nts locus may allow the application of this clone as a diagnostic probe in breeding programs as well as an entry point for the isolation of the nts gene.
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  • 39
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    Colloid & polymer science 269 (1991), S. 695-703 
    ISSN: 1435-1536
    Keywords: 2D spherulire ; growth ; linear temperature field ; calculus of variations ; theory and experiments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A 2D spherulite grows in a linear-temperature field from a nucleus of polypropylene at (0,0). The growth lines and the growth fronts are computed by the calculus of variations. The agreement between theory and experiment is satisfactory
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  • 40
    ISSN: 1573-5036
    Keywords: carbohydrate metabolism ; DNA-P ; growth ; nitrogen metabolism ; pyramin ; respiration ; RNA-P, sugarbeet pathogenic fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Effects of different concentrations of active ingredient of the herbicide pyramin on metabolic activities of Fusarium solani and Sclerotium rolfsii were examined. High concentrations of this herbicide (1000 and 2000 μg mL-1 for F. solani and 100 and 200 μg mL-1 for S. rolfsii) had inhibitory effects on the metabolic activities of both fungi. These were demonstrated by significant decreases in growth, and increases in rates of CO2 evolved, O2 consumed and keto acids produced. These were accompanied by increased rates of sugar, nitrate and inorganic phosphorus absorption as well as lowered rates of synthesis of carbohydrates and insoluble nitrogenous (including protein) and phosphorus (including RNA-P and DNA-P) compounds. In addition, rates of excretion of both nitrogen and phosphorus fractions by the mycelial mats were increased. A concentration of 25 μ g mL-1 exerted little or no effect on the metabolic activities of these fungi, although S. rolfsii was somewhat sensitive to this concentration.
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  • 41
    ISSN: 1573-5036
    Keywords: growth ; light response ; N uptake ; photosynthesis ; respiration ; rice (Oryza sativa L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to understand more clearly the dynamics of rice (Oryza sativa L.) yield development in relation to N fertilization, a field experiment was conducted in Laguna, Philippines. The soil, a Maahas clay (Aquic Hapludalf), was flooded, puddled, and then planted with 20-day-old seedlings of IR64 rice. Treatments were six combinations of N fertilizer timing and method: (1) 0 N control; (2) prilled urea broadcast at 15 and 60 days after transplanting (DAT) (BR-LATE); (3) prilled urea injected with a spring auger applicator at 15 DAT and broadcast at 60 DAT (INJ-LATE); (4) prilled urea broadcast and incorporated at 0 DAT and broadcast at 40 DAT (BR-EARLY); (5) urea super granules (USG) manually deep-placed at 3 DAT and prilled urea broadcast at 40 DAT (DP-EARLY); and (6) USG manually deep-placed at 3 DAT (DP). Except for the control, all treatments received a total of 58 kg N ha-1. Yield results were consistent with those of other experiments, namely, DP had the highest yields, the early-split treatments (BR-EARLY and DP-EARLY) were second best, followed by the late-split treatments (BR-LATE and INJ-LATE), with the control having the lowest yield. Sequential harvest results showed that the advantages of DP in terms of dry matter assimilation, tillering, and leaf area index (LAI) were expressed later in the season. For all treatments, midday net CO2 assimilation (Ac) peaked around 48 DAT, approximately panicle initiation. Grain yield was highly correlated with midday Ac at panicle initiation and flowering but not at other growth periods. Rates of midday Ac and night respiration depended strongly on shoot N content. We conclude that N application method and timing should be designed to give high shoot N content at panicle initiation and flowering, and that DP satisfied this requirement best among the treatments tested.
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  • 42
    ISSN: 1573-5060
    Keywords: Elaeis guineensis ; oil palm ; crown disease ; physiological disorder ; growth ; yield ; genetic predisposition ; mineral nutrition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crown disease was investigated in oil palm families derived from four sources of (Deli) dura female and three sources of pisifera male parents. These families were planted both at Bah Lias (after rubber) and Gunung Melayu (after oil palm) in North Sumatra. We observed the first symptoms on emerging leaves at 8 months after planting; severity reached a peak at 12 months and diminished gradually until virtually no new leaves were affected in palms older than 35 months. At Gunung Melayu crown disease, in terms of severity score on newly emerged leaves and percentage affected palms, was about double that at Bah Lias. At both locations it was highest in material exclusively bred in Indonesia. In this material crown disease significantly retarded vegetative growth, and reduced cumulative yield of oil for the first 6 years of production by about 4.5% with the highest loss in the first year. The relation between crown disease and nutritional status was inconsistent, but a crop rotation effect on crown disease merits further research. The contribution of the dura parents to crown disease was more evident than that of the pisifera. Screening of genotypes appears to be the most effective method of control of crown disease.
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  • 43
    ISSN: 1573-5036
    Keywords: aluminium toxicity ; growth ; magnesium nutrition ; nutrient uptake ; root length ; soil acidity ; Sorghum bicolor (L.) Moench
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An experiment to study the effects of Mg nutrition on root and shoot development of the Al-sensitive sorghum (Sorghum bicolor (L.) Moench) genotype CV323 grown in pots of sandy loam under different acid soil stress is reported. This experiment had a factorial design: four rates of liming were combined with four rates of Mg fertilization. When no Mg was added, the pH of the soil solutions (collected in ceramic cups) increased from 4.0 (unlimed) to 4.2, 4.7 and 5.9 at the increasing rates of liming. After 30 days of growth dry matter yields of the limed treatments were 40%, 115% and 199% higher than that of the unlimed treatment. Without liming and at the highest liming rate, adding Mg did not affect plant biomass significantly. At the two intermediate levels of liming, however, 11.3 mg extra Mg per kg soil increased dry matter yield to the same levels as found at the highest liming rate. Concentrations of Mg in the soil solution rose after Mg was added and fell when lime was added, but adding both Mg and lime increased Mg concentrations in the plant shoots. In plants of the limed treatments, dry matter yield was correlated closely with the Mg concentration in the shoot. This was not so in the unlimed treatment. Furthermore, in the unlimed treatments root development was inhibited, but reduced Mg uptake by the plants resulted mainly from the direct effect of Al- (or H-) ions in the soil solution rather than from impaired root development. It is concluded that Mg fertilization counteracted the interfering effects of Al- and H ions on Mg uptake.
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  • 44
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    Plant and soil 137 (1991), S. 201-207 
    ISSN: 1573-5036
    Keywords: CO2 uptake ; growth ; monovalent ions ; osmotic adjustment ; water relations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In view of the need to exploit saline water resources in agriculture in arid zones, we investigated the salt tolerance of Opuntia ficus-indica in plants growing in solution culture. Salt (NaCl) was added in concentrations ranging from 5 (control) to 200 mol m-3. Cladode growth was sensitive to salinity, being 60% of the control at 50 mol m-3 NaCl. The root-to-stem ratio decreased significantly only at 200 mol m-3. Various other parameters were studied, such as water content, Na, K and Cl content, osmotic pressure, and CO2 uptake. Of these parameters the decreases in cladode water content and CO2 uptake were related to the decrease in cladode growth. Raised salinity increased cladode osmotic pressure, which was associated with tissue dehydration. We concluded that osmotic adjustment does not occur in prickly pear under salt stress.
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  • 45
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    Plant and soil 134 (1991), S. 243-254 
    ISSN: 1573-5036
    Keywords: dry matter partitioning ; growth ; lettuce ; N response ; potato ; quality ; response surface model ; zucchini squash
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plants grown at 5 nitrate (N) levels ranging from 2–36 mmol L−1 for lettuce and 2–43 mmol L−1 for zucchini squash and potato were harvested over the growth period to maturity at a minimum of 2 week intervals. Gamma × cubic response surface models fitted actual dry matter growth data quite precisely (R2〉0.98) from which growth and dry matter partitioning could be derived. Total dry matter growth was very responsive to N and maximum growth was predicted to occur at an N level of 14.2 mmol L−1 for lettuce, 18.1 mmol L−1 for zucchini squash and 11.6 mmol L−1 for potato. Growth declined at higher N levels for all species. For zucchini squash, both high and low N levels which reduced growth increased partitioning of dry matter to fruit. For potato, partitioning of dry matter to tubers tended to increase at high levels of N, particularly at maturity. For lettuce, the N level producing the highest dry matter yield partitioned the highest ratio of dry matter to head. The highest fresh yields of zucchini squash fruit, lettuce head and potato tubers were recorded at N levels of 14, 5 and 11 and 7 mmol L−1 respectively. The effect of N on quality followed a similar trend.
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  • 46
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    Euphytica 52 (1991), S. 19-23 
    ISSN: 1573-5060
    Keywords: Musa acuminata ; Musa balbisiana ; banana ; plantain ; cytoplasm ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Concerns over yield declines in bananas and plantains due to the spread of Black Sigatoka disease in Musa have drawn attention to the collection of Musa germplasm and its use in conventional and biotechnological improvement programs. This report demonstrates the use of chloroplast DNA (cpDNA) restriction fragment length polymorphisms (RFLPs) for differentiating cytoplasms of various Musa clones. DNA was extracted from lyophilized leaf blade tissue and digested with either Eco R1, Hind III, Bam H1 or Pst I. Southern blots onto nylon membranes were probed with radioactively labeled heterologous orchid and lettuce cpDNA fragments. Among the 14 Musa clones examined, a single balbisiana and four acuminata-type cytoplasms were differentiated. The ability to distinguish between cytoplasms and to place plants within a cytoplasmic grouping demonstrates the usefulness of RFLP technology in evaluating diversity and determining the ancestry of Musa clones.
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  • 47
    ISSN: 1573-5060
    Keywords: barley ; Hordeum vulgare ; RFLP ; chromosome arm ; molecular marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A set of 14 probes from wheat cDNA clones was used to search for restriction fragment length polymorphism (RFLP) in six barley lines. The degree of polymorphism among the lines varied greatly between probes and between the various restriction enzymes. Two probes revealed a high degree of polymorphism in all probe/enzyme combinations. Seven of 14 probes did not reveal RFLP. The average level of polymorphism based on all 840 pairwise comparisons was 14.0%, which is higher than has been reported in wheat, but lower than in maize, rice, potato and lettuce. Most of the probes that detected RFLP correspond to sites on the long arms of wheat chromosomes.
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  • 48
    ISSN: 1573-5060
    Keywords: Beta vulgaris ; beet ; RFLP ; Beta taxonomy ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Clones derived from Beta vulgaris and Beta maritima were assayed for their ability to detect restriction fragment length polymorphisms (RFLPs) in different beet accessions. The clones able to detect polymorphism were used as genetic markers to assess the degree of genetic variation existing between and within different species of the genus Beta. The data support the current taxonomy of the Beta vulgaris section, while the great genetic similarity found between Beta webbiana and Beta procumbens indicates that they could belong to the same species. Enough variation was found between Beta vulgaris cultivars, allowing the isolation of a sufficient number of genetic markers for the construction of detailed genetic maps.
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  • 49
    ISSN: 1573-5060
    Keywords: Malus ; apple ; genetic mapping ; isozymes ; DNA techniques ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A recently initiated collaborative project involving apple breeders in seven European countries is described. The objective is to improve the European apple crop by molecular-aided breeding to increase efficency and reduce the time-scale in breeding for resistance, tree habit and fruit quality. The strategy adopted provides a model for similar studies in fruit, forest and other woody species. The project is based on progenies from a small number of crosses involving many important agronomic genes. Replication of these reference progenies by vegetative propagation will enable studies to be carried out simultaneously in each country. By developing a range of molecular markers, including isozymes, RFLPs and sequence-tagged DNA probes, an integrated molecular map is being constructed for use in a wide range of breeding and genetic studies. Construction of a database recording many mapped molecular markers will enable efficient exploitation of data in future genetic, breeding and physiological studies of apple. Aspects of the adopted strategy, techniques and management are discussed in the context of mapping genes in perennial crop genomes.
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  • 50
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    New forests 5 (1991), S. 147-156 
    ISSN: 1573-5095
    Keywords: bareroot and container seedlings ; survival ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ponderosa pine, Jeffrey pine, and Douglas-fir seedlings were planted in container or bareroot form at three elevations in northern California. At the lowest elevation (762 m), container seedlings of ponderosa pine were significantly taller than bareroot seedlings at ages 4 and 10, and had breast-height diameters that were significantly larger than bareroot counterparts at age 10. Survival of Douglas-fir container seedlings was significantly greater than that of barefoot seedlings for all ages tested. At the mid-elevation site (1220 m), container seedlings of ponderosa pine and Douglas-fir were significantly larger in breast-height diameter than bareroot seedlings at age 10. Douglas-fir container seedlings survived significantly better at all ages than barefoot seedlings. At the highest elevation (1662 m), seedling height and diameter did not differ significantly, but survival of container seedlings was significantly higher than barefoot seedlings for both pine species at all ages tested.
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  • 51
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    Hydrobiologia 213 (1991), S. 99-111 
    ISSN: 1573-5117
    Keywords: age ; growth ; feeding ; reproduction ; Leuciscus svallize ; plastic adaptations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The age, growth, feeding habits and reproductive biology of Leuciscus ‘svallize’ in artificial lake Kremasta (Greece), are described. There is a considerable similarity in the feeding and reproductive characteristics between L. ‘svallize’ and L. cephalus and also a similarity in the life span, growth patterns and age of maturation between L. ‘svallize’ and some southern L. cephalus populations, which may suggest similar responses of closely related taxa to similar conditions.
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  • 52
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    Hydrobiologia 216-217 (1991), S. 229-234 
    ISSN: 1573-5117
    Keywords: Hydroids ; growth ; reproduction ; control mechanisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of variations in temperature, salinity and water movement on the laboratory growth and reproduction of a single clone of the bougainvilliid hydroid Clavopsella michaeli were examined. Unfavourable conditions resulted in reproducible oscillations of growth rate. They ranged from overall stimulatory effects (hormesis) to irreversible inhibition and shrinkage of colonies. Exposure to low concentrations (0.1–5 µg l−1) of copper and mercury ions produced similar responses, which were therefore regarded as non-specific. A control mechanism, dampening these growth rate oscillations, supported higher colonial tolerance, that is adaptation, to environmental stresses of low intensity. When the counteractive capacity of the control mechanism was exceeded, inhibition occurred. Higher frequencies of gonozooids were observed in stressed colonies and were interpreted as an adaptive response.
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  • 53
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    Hydrobiologia 221 (1991), S. 91-106 
    ISSN: 1573-5117
    Keywords: agarophyte ; cultivation ; Gelidium ; growth ; hydrodynamics ; mariculture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gelidium fronds were grown in the sea under a variety of experimental conditions: on rigid, damped and tensioned test farms of various designs, in calmer and more turbulent habitats, at various depths, with and without commercial fertilizer supply. Initially, the effectiveness of a given cultivation strategy was based on the survival and growth of the fronds, here termed ‘bio-assay’ mariculture. Ambient seawater temperature, nutrient availability, hydrodynamics and other environmental parameters were measured periodically. In-the-sea irrigation of test plants with commercial fertilizers was apparently effective, at least with some farm designs, and when ambient nutrient levels were low. Under optimal conditions, achieved through experimental manipulation of farm design, specific growth rates of over 2% per day were recorded. However, considerable variation in growth rates and in plant performances was observed. It was not always possible to correlate these variations with design modifications or other experimental parameters. In view of these findings, we have reviewed our initial ‘bio-assay’ approach, namely the assumptions about the design and operation of farm structures and their interactions with the water and the fronds. Methods were developed to quantify these interactions. We advocate a quantitative, ‘hydrodynamic’ approach in developing an effective cultivation strategy for gelidioid algae and are optimistic about progressing from test-to-commercial scale farms in the near future.
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    Hydrobiologia 221 (1991), S. 125-135 
    ISSN: 1573-5117
    Keywords: cultivation ; efficiencies ; growth ; light ; production ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Numerous species of seaweed have been successfully cultivated in the sea for commercial purposes. Although considerable experimental work has been done on on-shore cultivation systems, none of these has yet proved to be economically viable on a sustained basis; nevertheless, such cultivation systems offer the potential for productivities greater than can be achieved in other systems. In on-shore systems, factors other than light can be controlled and provided at saturation levels. As density of biomass is controllable, all the light entering the cultivation system is absorbed. This results in efficient conversion of light energy to biomass when only light is limiting; moreover, density, rather than growth rate, is the major factor determining productivity. As growth of seaweeds in on-shore systems is only vegetative, there is no interruption for reproduction or maturation of the plants, and all of the net production can be recovered. Seaweeds have, though, relatively low percentage carbon composition, compared with terrestrial plants, and this may result in apparent high productivities based on dry matter.
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  • 55
    ISSN: 1573-5117
    Keywords: Galaxias occidentalis ; Galaxiidae ; otoliths ; age ; growth ; body size ; diet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The size and age composition, growth and diet of the western minnow, Galaxias occidentalis, in the southern branch of the Collie River in south-western Australia were determined from samples collected at approximately monthly intervals between January 1984 and December 1986. The prominent, narrow hyaline zone on otoliths was shown to be formed annually and thus valid for ageing these fish. An examination of the otoliths of 4711 fish indicated that 69.2 and 26.8% of the fish caught belonged to the 0 + and I + age classes, respectively. While the vast majority of the remaining fish represented the II + age class, a few III + and IV + and one V + fish were also recorded. Growth was seasonal, being greatest in spring and autumn and effectively ceasing in winter. Females began to grow faster than males during their first autumn of life. The respective mean lengths of males and females were ∼ 70 and 75 mm at the end of the first year and ∼ 90 and 100 mm at the end of the second year. The largest fish caught was a III + female measuring 163 mm and weighing 32.0 g. The respective von Bertalanffy growth curve parameters for L∞, K and t0 were 109, 0.758 and - 0.338 for males, and 124, 0.762 and - 0.223 for females. Although the diet of G. occidentalis was always dominated by small terrestrial fauna, and particularly insects, conspicuous seasonal differences were found between the more important of the other components. Data on body size, growth and diet of G. occidentalis are compared with those recorded for other galaxiids.
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  • 56
    ISSN: 1573-5117
    Keywords: Hydrozoa ; Sertularia ; larval settlement ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Aspects of the life cycle of the hydroid Sertularia cupressina were monitored over a period of 2 y using settlement panels. The reproductive period, determined by the presence of acrocysts and larval settlement, was November to January. Recently settled hydroids were more abundant on horizontal upward facing surfaces than on horizontal downward facing or vertical surfaces. The spatial dispersal of settlers was random. S. cupressina was a conspicuous and persistent component of fouling communities developing on both upward facing and vertical panels over 2 y. Following settlement in winter 1979–80, hydrocauli reached their greatest height between November 1980 and April 1981, but fouling and breakage of stems occurred during spring 1981. Regeneration from the tips of broken stems and from hydrorhizae occurred in June and July 1981.
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  • 57
    ISSN: 1573-5117
    Keywords: growth ; stock assessment ; inflow-rotifer-recruitment relationship ; zooplankton consumption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Catch analyses indicate increases in Mirogrex standing stock (exploited phase) from 〈 2000 tonnes in the early 1950's to 〉 12000 tonnes in the mid-1980's and a declining abundance after 1986. Brood strength is related to concentrations of influxed organic matter which provide larval food. Estimated von Bertalanffy growth parameters (L ∞ = 164 mm TL, K = 0.25 per yr for males; L ∞ = 198 mm, K = 0.14 for females) denote a low production/biomass ratio compared to temperate cyprinid populations, and a low rate of food consumption. From estimates of zooplankton production in recent years, an average predation of 〈 3 % fish weight/day is calculated. The decline in zooplankton biomass since 1970 is attributed to overgrazing by Mirogrex at high stock levels. At a lower biomass, the fish population can promote ecosystem stability. Mortality estimates from Mirogrex size distribution in a period of low exploitation were ∼ 1.5 times K-values for both males and females. Similar M/K ratios were estimated for two unexploited cyprinid stocks (Phoxinus phoxinus and Acanthobrama lissneri).
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  • 58
    ISSN: 1573-5117
    Keywords: Corbicula fluminea ; growth ; density ; sewage treatment plant ; Ceriodaphnia dubia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The biology of Corbicula fluminea, the Asiatic clam, in the Vermilion River, Louisiana, as affected by sediment, dissolved oxygen (DO) levels, and sewage treatment plant (STP) effluents was investigated. A point source of high DO water to the Vermilion River established a gradient of DO that decreased as the river moved towards the Gulf of Mexico. Lowering DO levels were exacerbated by municipal sewage treatment plant discharges in the 20 km reach studied. Low dissolved oxygen was associated with reduced Corbicula density in the river and 30-day in-stream growth studies (weight and length) demonstrated that low DO inhibited growth. Generally, if DO was 〈 1.0 mg l−1 in sediment pore water and/or 〈 3.0 mg l−1 at the sediment-water interface, growth was significantly impaired (p 〈 0.05). Corbicula experienced substantial mortality near the STP discharges (up to 70% in 30 days) and laboratory toxicity tests with Ceriodaphnia dubia, a sensitive cladoceran, also strongly suggested discharges were chronically toxic at 6.25–25.0% effluent. Respiration experiments along with environmental measurements of DO, temperature, and STP discharge chemistry support a hypothesis that clam populations are adversely affected by the suite of environmental conditions present in the Vermilion River. Further, growth studies were consistent with observed population densities in situ.
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  • 59
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    Hydrobiologia 225 (1991), S. 193-197 
    ISSN: 1573-5117
    Keywords: Rutilus rutilus ; Daphnia galeata ; predator-induction ; growth ; reproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Both the size of Daphnia galeata primiparae and the average egg volume were smaller in individuals reared from neonata in water inhabited for the preceding 24 hours by Y-O-Y roach when compared with control animals reared in the same water with the exclusion of fish. The smaller size of experimental primiparae resulted from smaller increments in some instars and from the earlier maturation of about two-thirds of those that matured in the 4th instar, while the rest of the experimental animals and all the controls matured in the 5th instar. There were larger clutches and smaller eggs in experimentals when compared with the similar sized controls, but the difference hasn't proved to be statistically significant.
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  • 60
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    Plant cell, tissue and organ culture 26 (1991), S. 153-160 
    ISSN: 1573-5044
    Keywords: Actinidia deliciosa ; carbohydrates ; growth ; in vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in sugar composition (sucrose, glucose and fructose) of medium, callus, stem and leaves of in vitro proliferating explants of Actinidia deliciosa C.F. Liang, ‘Hayward’ were analyzed together with explant growth at 0, 15, 30, 45 and 60 days of culturing. Autoclaving hydrolyzes a small part of the initial sucrose of the medium into glucose and fructose. In presence of Actinidia explants the initial sucrose decreased to 32% after 15 days of culturing, to 4% after 30 days and to 0.08% at the end of the culture period (60 days). Sucrose increase in the explants did not parallel with its decrease in the medium. Sucrose presence in the explants was evident only during the last month of culturing. After 15 days of culturing a large increase of glucose and fructose was found in the medium but it did not equal the hydrolyzed sucrose. The level of these two monosaccharides remained stable in the medium until the 30th day, then significantly decreased in the second month of culture; neither were completely exhausted at the end of the culture. In the whole explant the highest amount of glucose and fructose was reached after 30 days of culturing. The balance of the three sugars in the medium-explant system, as % distribution of carbon atoms, showed a utilization throughout the whole culture period. Qualitative analyses performed on medium, callus and leaves at 0, 15, and 30 days of culturing revealed the presence of glucose and fructose only and no significant amounts of other hexoses or pentoses. Starch accumulation in the leaves was also observed throughout the culturing.
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    Plant cell, tissue and organ culture 25 (1991), S. 91-98 
    ISSN: 1573-5044
    Keywords: Actinidia deliciosa ; growth ; mineral nutrients ; in vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro growth of Actinidia deliciosa C.F. Liang, cv Hayward and changes in mineral composition of the medium and in the different parts of the explants (callus, stem and leaves), were analyzed after 0, 15, 30, 45 and 60 days of culturing in each of three successive 60 days subcultures. Fresh (FW) and dry weight (DW) of the explants increased mainly during the first 30 days of culturing, with a predominant increase of FW in leaves and an equal distribution in DW in callus and leaves. Stem FW and DW changes were lower than those observed with callus and leaves. As FW and DW of the explants increased the FW and DW of the medium decreased. The presence of the explants induced a large decrease of medium pH during the first 15 days of culturing followed by a return to the initial level at the end of the culturing. The initial P content of the MS medium was insufficient for the long term culturing, as after 30 days of culturing almost all (94.5%) the P present in the medium was absorbed by the explants and evenly distributed in their different parts. During the first month, 85% of the initial N was absorbed. At the end of the culture only 2% of the initial P and 5% of N remained in the medium. These two elements were equally distributed in callus and leaves during the first month of culturing, while in the last 30 days they increased only in the callus. MS medium initial concentrations of K, Mg, Ca, Fe, Zn, Mn and Cu were sufficient for 60 days explants growth. Almost all these elements were absorbed during the first 30 days of culturing. Their distribution in the different parts of the explant was uneven throughout the culture period. Callus tissue was the main site for accumulation of all these mineral elements.
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  • 62
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    Colloid & polymer science 269 (1991), S. 689-694 
    ISSN: 1435-1536
    Keywords: 2D spherulite ; growth ; field of growth rate ; calculus of variations ; linear growth rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract We propose to take the calculus of variations in order to compute the shape of a growing 2D spherulite in an uniaxial field of growth rate. We are concerned with the growth line (a path that is traveled in the shortest possible time from nucleus to a point (x1, y1), where a molecule just crystallizes) and the growth front (the times between spherulite and supercooled material). The Euler differential equation—a result of the calculus of variations—is derived for all uniaxial growth ratesv (x). Here we especially investigatev(x)=px+q.
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  • 63
    ISSN: 1572-9680
    Keywords: speargrass ; Imperata cylindrica ; Gliricidia sepium ; Leucaena leucocephala ; hedgerow species ; shading ; growth ; development ; weed control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field studies carried out in a forest transition site on a tropical Alfisol in southwestern Nigeria, show that shading by gliricidia (Gliricidia sepium (Jacq.) Steud) and leucaena (Leucaena leucocephala (Lam.) de Wit) hedgerow species reduced density of speargrass (Imperata cylindrica (L.) Raeuschel) by 67% and 51%, respectively. Shoot biomass of speargrass decreased by 81% and 78% in gliricidia and leucaena hedgerows, respectively. Reduction in speargrass rhizome biomass in gliricidia plots was 96% while rhizome reduction in the leucaena plots was nearly 90%. Rhizome mortality was significantly higher in gliricidia plots than in leucaena and control plots. The bulk of rhizomes was found between 10 cm and 20 cm of soil depth but rhizome did not penetrate further than 30 cm depth in the Alfisol in which this study was carried out. Gliricidia was better than leucaena hedgerow species in suppressing speargrass.
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    Hydrobiologia 212 (1991), S. 11-17 
    ISSN: 1573-5117
    Keywords: Artemia (Tuticorin strain) ; organic waste ; agricultural waste ; salinity ; growth ; fecundity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Artemia sp (Tuticorin strain) was cultured at a density of 250 individuals 1−1 at 35‰, 45‰, 60‰, 75‰ salinity using five combinations of groundnut oil cake, decayed cabbage leaves, single superphosphate and Baker's yeast as feed. Effects on survival, growth, and fecundity were noted.
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    Journal of human genetics 36 (1991), S. 325-329 
    ISSN: 1435-232X
    Keywords: genetic linkage map ; RFLP ; human aldolase B ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The probe containing the exon 9 of human aldolase B gene revealedMspI polymorphism involving two fragments 6.5 and 3.0 kb long with the high frequency of heterozygosity (21%). The two alleles can be distinguished efficiently by the DNA PCR-restriction fragment length polymorphism procedure.
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    Cytotechnology 5 (1991), S. 165-171 
    ISSN: 1573-0778
    Keywords: growth ; hybridoma ; inoculum age ; kinetics ; production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract To determine the influence of the inoculum age on the kinetics of hybridoma growth and metabolism, spinner flasks have been inoculated with cells previously propagated in T flasks for 43, 52, 62 and 71 hr respectively. Increasing the age of the inoculum is found to result in a longer lag phase, in a lower maximum specific growth rate and in a reduced maximal cell density. During the growth phase specific rates of glucose and glutamine uptake and of ammonia and lactate production are similar. However, with the older inoculum, much higher metabolic activities are observed during the lag phase. The production of antibodies is delayed with increasing inoculum age, but the final antibody concentrations are similar, which indicates a higher specific antibody production rate when inoculating with older cells.
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