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  • Articles  (136)
  • Triticum aestivum  (60)
  • wheat  (57)
  • calcium  (49)
  • Springer  (136)
  • 1990-1994  (136)
  • 1990  (136)
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  • Articles  (136)
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  • 1990-1994  (136)
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  • 1
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    Springer
    Cellular and molecular life sciences 46 (1990), S. 962-970 
    ISSN: 1420-9071
    Keywords: Acid deposition ; reproduction ; birds ; insects ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Acidification in aquatic habitats reduces the reproductive success of both piscivorous and non piscivorous birds, mainly by reducing the food supply. Piscivorous birds find some compensation in an increased transparency of the water, non piscivorous birds in less competition for invertebrate prey by fish. Acidification in forests often has large impacts on insect populations but how this affects forest birds is unknown. Some woodpeckers and nuthatches temporarily benefit from an increase in standing dead timber. In advanced stages of forest dieback the breeding density of forest birds is very much reduced, but species of open woodland increase. Calcium deficiency reduces the reproductive output of some passerine species, but the extent of this phenomenon is unknown. Increased exposure to toxic metals has reduced the reproductive success of some lake dwelling species. It is difficult to assess the effect of acid precipitation on birds since acidification affects ecosystems in many ways, the evidence is largely correlative and reliable estimates of the population size are often lacking. Future studies should concentrate on carefully selected indicator species suitable for detailed data collection.
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  • 2
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    Cellular and molecular life sciences 46 (1990), S. 1063-1065 
    ISSN: 1420-9071
    Keywords: Dictyostelium ; cell communication ; biological rhythms ; oscillations ; cAMP ; folate ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With a light-scattering technique, two novel rhythms were discovered in cell suspensions ofDictyostelium discoideum. One is a damped oscillation with a period of 2 to 2.5 min (at 23°C) induced by folate in EDTA-dissociated undifferentiated cells. The other is a sinusoidal oscillation with a period of about 12 min occasionally observed with late differentiated cells. Obviously, the repertoire of rhythms of this simple eukaryotic organism is larger than previously assumed.
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  • 3
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    Cellular and molecular life sciences 46 (1990), S. 41-48 
    ISSN: 1420-9071
    Keywords: Cardiac muscle ; cell damage ; calcium ; calcium-paradox ; oxygen-paradox ; oxygen radicals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The O2− and Ca2+-paradoxes have a number of features in common and it is suggested that release of cytosolic proteins in both paradoxes is initiated by the activation of a sarcolemma NAD(P)H dehydrogenase which can generate a transmembrane flow of H+ and e− and also oxygen radicals or recox cycling which damage ion channels and membrane proteins (phase I). Entry of Ca2+ through the damaged ion channels then exacerbates the damage by further activating this system, either directly or indirectly, and the redox cycling and/or oxygen radicals cause further damage to integral and cytoskeletal proteins of the sarcolemma resulting in microdamage to the integrity of the membrane (phase II) and the consequent release or exocytosis of cytoplasmic proteins and, under specialised condition, the blebbing of the sarcolemma. The system may be primed either by removal of extracellular Ca2+ or by raising [Ca2+]i by a variety of measures, these two actions being synergistic. The system is initially activated in the Ca2+-paradox by the membrane perturbation associated with removal of extracellular Ca2+; prolonged anoxia in the metabolically active cardiac muscle causes a depletion of the ATP supply, particularly in the absence of glucose, and hence a rise in [Ca2+]i in phase I of the oxygen paradox with the consequent activation of the NAD(P)H oxidase at the sarcolemma. Oxygen radicals are probably generated in both paradoxes and may have a partial role in the genesis of damage, but are not essential in the Ca2+-paradox which continues under anoxia. Massive entry of Ca2+ also activates an intracellularly localised dehydrogenase (probably at the SR) which produces myofilament damage by redox cycling.
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  • 4
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    Cellular and molecular life sciences 46 (1990), S. 26-40 
    ISSN: 1420-9071
    Keywords: Opiomelanocortin ; cyclic adenosine 3′,5′-monophosphate (cAMP) ; calcium ; phosphatidylinositol (PI) ; glucocorticoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adrenocorticotropin (ACTH), an opiomelanocortin peptide, is secreted from anterior pituitary corticotrophs upon stimulation with corticotropin-releasing hormone (CRH), arginine vasopressin (AVP) and several other neuropeptides. CRH, the most potent secretagogue of ACTH, stimulates ACTH secretion and biosynthesis by increasing the production of cyclic adenosine 3′,5′-monophosphate (cAMP) within corticotrophs. AVP, which is a weak secretagogue of ACTH but strongly potentiates CRH-stimulated ACTH secretion, operates through the phosphatidylinositol (PI) transduction pathway. Both CRH and AVP increase cytosolic free [Ca2+] within normal corticotrophs indicating a role for Ca2+ in ACTH secretion. Glucocorticoids inhibit ACTH synthesis by suppressing transcription of the proopiomelanocortin (POMC) gene and attenuate ACTH release by decreasing cAMP accumulation stimulated by CRH. This review focuses on the roles of these intracellular messengers in ACTH secretion from normal anterior pituitary cells in vitro, and discusses the possible interactions between the cAMP, calcium and PI transduction pathways. Future areas of research are suggested such as identification of protein substrates of cAMP-dependent and Ca2+-dependent kinases within normal corticotrophs and evaluation of their role in ACTH biosynthesis and secretion.
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  • 5
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    Biology and fertility of soils 9 (1990), S. 281-282 
    ISSN: 1432-0789
    Keywords: Surface sterilization ; Contaminants ; Sterile plant selection ; Axenic seedlings ; Triticum aestivum ; Trifolium pratense ; Trifolium repens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sterile seedlings are required for the investigation of interactions between microorganisms and plants. The present study was designed to develop a simple and reliable method for the selection of sterile seedlings by the use of liquid nutrient media, avoiding some of the disadvantages of solid media. The method of germinating surface-sterilized seedlings on solid water agar or nutrient media was compared with our method for sterility control in nutrient broth. Sterile plant selection in liquid media was the most sensitive method for detecting bacterial and fungal contaminants. Sterile plants grow with the same vigour as unsterilized plants and can be used for sterile plant experiments.
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  • 6
    ISSN: 1432-0983
    Keywords: Secale cereale ; Triticum aestivum ; Ribosomal RNA genes ; Mitochondrial DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial 18S and 5S ribosomal RNA (rRNA) genes of rye, plus a total of about 90 kilobase pairs of flanking DNA, have been cloned and maps of restriction enzyme cleavage sites have been constructed. Like their homologs from hexaploid wheat, the rye genes are closely linked and are part of a three-copy family of recombining repeats (the “18S/5S repeat”). The rye repeat probably also contains a mitochondrial tRNAfMet gene, which the wheat repeat is known to carry. However, despite the overall organizational similarity between the wheat and rye 18S/5S repeats in the immediate vicinity of their coding regions, extensive rearrangement of flanking sequences has taken place during evolutionary divergence of the two species. Our data provide additional support for an emerging picture of plant mitochondrial genomes as evolving much more rapidly in structure than in sequence.
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  • 7
    ISSN: 1432-0983
    Keywords: Triticum aestivum ; Mitochondrial DNA ; Chloroplast DNA ; ATPase alpha subunit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An internal part of the chloroplast atpA gene has been identified in the mitochondrial DNA of Triticum aestivum. It is located near the 18S-5S ribosomal genes and partially contained within a repeated sequence. Comparison of the transferred sequence with the original ct sequence reveals several nucleotide changes and shows that neither 5′ nor 3′ ends are present in the mt genome. No transcript of this region could be detected by Northern analysis. This sequence is present in mitochondrial genomes of other tetraploid and diploid species of Triticum, also in the vicinity of the 18S-5S ribosomal genes, suggesting a unique transfer event. The date of this event is discussed.
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  • 8
    ISSN: 1436-5073
    Keywords: calcium ; cryptand 2.2.1 ; fluorimetric determination ; ion-pair extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A fluorimetric study on the extraction of calcium into 1,2-dichloroethane as an ion-pair, formed between the cryptand 2.2.1-calcium complex and the eosinate anion, is described. Optimum conditions for extraction are established and a new fluorimetric determination of ultratraces of calcium is proposed. A linear working range from 1.5 ng ml−1 (detection limit) to 100 ng ml−1 of calcium and a relative standard deviation of ± 2.9% at the 70 ng ml−1 level are obtained. The equilibrium constants involved in the extraction process have been calculated and refined by the Letagrop-DISTR program. The proposed method has been tested for the direct determination of calcium in sugars.
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  • 9
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    Mycopathologia 111 (1990), S. 181-189 
    ISSN: 1573-0832
    Keywords: mycotoxin ; ochratoxin ; Penicillium ; storage ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Eleven-kilogram parcels of HY-320 wheat, a cultivar of the new Canada Prairie Spring class, were kept at 15 and 19% initial moisture contents (IMC) in simulated storage in a Manitoba farm granary for 60 weeks to determine biotic and abiotic changes and mycotoxin production. Ochratoxin A reached a maximum of 0.24 ppm by week 20 in the 19% IMC wheat, but was absent in the 15% IMC wheat; no other mycotoxins were detected. Temperature, moisture content, O2 and CO2 levels, fat acidity values, seed germination, microfloral incidence and abundance, and the presence of other mycotoxins were monitored. Principal component analysis of all variables showed that the first principal components accounted for 32–41% of the system variability, and contained the ochratoxin A variable. Ochratoxin A was produced in moist grain that had decreased seed germination andAltermaria activity, and high fungal activity byPenicillium andAspergillus versicolor. Compared to other stored cereals previously studied, HY-320 wheat would be ranked in a low-risk category for mycotoxin formation, based on the ochratoxin A levels observed.
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  • 10
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    Nutrient cycling in agroecosystems 24 (1990), S. 77-84 
    ISSN: 1573-0867
    Keywords: Brussels sprouts ; calcium ; calcite ; elemental sulphur ; gypsum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Studies of crop response to Ca fertilizers are generally few as well as information concerning the Ca nutrition of Brussels sprouts (Brassica oleracea var.gemmifera). Six field studies were conducted, over three years, to determine yield response of Brussels sprouts to soil applied gypsum (CaSO4.2H2O), lime (calcite), and elemental S on sandy loam to loamy sand Orthic Podzols in Prince Edward Island. Relative yield of marketable (0–32mm) Brussels sprouts were related to soil ammonium acetate extractable Ca (r = 0.71). The highest yields (11 to 13 t ha−1) were associated with an extractable Ca of above 400µg g−1 soil, while a Ca level below 400µg reduced yield by 20%. Highest marketable yields were associated with a Ca level in the leaf tissue (in upper mature leaves at sprout formation) of above 2.2% (w/w) (r = 0.55), this in turn was associated (r = 0.87) with an extractable soil Ca above 400µg g−1 soil. Calcite and elemental S did not influence yield or mineral content. Gypsum, as expected increased leaf S content, but leaf tissue S levels were not related to marketable yield. Slight decreases in soil pH due to increasing gypsum rate (0.5–4.3 t ha−1) were associated with changing accumulations of B, Mn, Fe, Ca, and Zn in the leaf tissue. Gypsum had little effect on soil porosity and structure indices, but changing pH (in both gypsum and lime treatments) significantly influenced soil microbial biomass.
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  • 11
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    Nutrient cycling in agroecosystems 21 (1990), S. 163-166 
    ISSN: 1573-0867
    Keywords: Critical level of Zn ; alkaline soils ; Zn-deficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field experiments were conducted at 32 locations, chosen for their wide range in DTPA extractable Zn, to determine the critical deficiency level of Zn for predicting response of wheat to Zn application. Soil application of 5.6 kg Zn ha−1 significantly increased the grain yield in deficient soils. Soil extractable Zn was significantly related with per cent grain response and absolute grain yield. Both the graphical and statistical methods of Cate and Nelson indicated the critical level to be 0.75 mg kg−1 soil of DTPA extractable Zn. This level gave a predictability value of 82 per cent.
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  • 12
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    Nutrient cycling in agroecosystems 22 (1990), S. 147-159 
    ISSN: 1573-0867
    Keywords: Sulfate ; elemental sulfur ; leaching ; ryegrass ; wheat ; greenhouse experiments ; split application
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three factorial experiments with four replications were conducted in a greenhouse to examine the effectiveness of gypsum, elemental sulfur (ES powder) and three S containing N fertilizers, viz., ammonium sulfate (AS), urea + ES, and Ureas (20% AS and 80% urea). All experiments were conducted twice in different years. In the first experiment with uncropped soil, the effects of soil type, leaching rate (2.3 and 6.9 mm water per day) and urea addition on sulfate leaching losses were studied. Leaching losses decreased in the order Ureas 〉 ammonium sulfate (AS) 〉 gypsum ≫ urea + ES. Increasing the leaching rate greatly increased sulfate losses from both soils. Losses were greater in the sandy Typic Hapludoll than in the clayey Oxic Paleustalf. Sulfate adsorption was found to decrease strongly with rising the pH in both soils. Hydrolysis of urea temporarily raised the pH of the soil, thereby increasing the sulfate leaching losses. In the second experiment the effects of S rate (0–65 mg per kg soil), split application and leaching rate (0 and 2.3 mm per day) on sulfate leaching losses and ‘apparent S recovery’ (ASR) by three successive cuts of ryegrass (Lolium perenne L.) were studied. Herbage yield more than doubled when S was applied. The effectiveness of the sulfate fertilizers was greater when S was split-applied than given all at once. With split applications the ASR decreased in the order: Ureas 〉 AS 〉 gypsum 〉 urea + ES 〉 ES powder. ES fertilizers were least effective, because the oxidation rate of ES to sulfate was clearly too slow. In the third experiment the effects of S rate (0–40 mg per kg soil) and split application on sulfate leaching losses and ASR in the grain of wheat (Triticum aestivum L.) were studied under leaching conditions (2.3 mm per day). Grain yield increased strongly due to S application. Split application greatly increased the effectiveness of the sulfate fertilizers and appeared to be an effective tool in satisfying the S need of the crop under leaching conditions. Again, ES fertilizers were least effective, because the oxidation rate of ES was too slow to meet the S demand of the crop. In all experiments leaching losses of sulfate from the ES fertilizers were smaller than from the sulfate fertilizers.
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  • 13
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    Nutrient cycling in agroecosystems 23 (1990), S. 147-150 
    ISSN: 1573-0867
    Keywords: Ammonium sulphate ; diammonium phosphate ; urease inhibitors ; hydrolysis ; pH ; calcium ; phosphogypsum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The extent of ammonia volatilization losses from urea, ammonium sulphate (AS), and diammonium phosphate (DAP) were determined in soil incubation studies. The effects of some urease inhibitors (thiourea, hyroquinone, 2–4 dinitro phenol and boric acid) and CaCl2 and phosphogypsum additions on ammonia loss from urea were also studied. Total ammonia volatilization losses were 32.6%, 3.1% and 2.3% of the N applied to the soil as urea, AS and DAP, respectively. Among the chemicals examined in the study, 500 mg H3BO3 in 1 kg of the soil decreased the ammonia loss from urea by 21% in comparison with the control. When 50 mg/kg soil of thiourea, 2–4 dinitro phenol or hydroquinone were applied, ammonia volatilization losses were found to be 10%, 3% and 0% less than urea applied alone, respectively. When 2500 mg CaCl2 was applied to 1 kg of soil with urea, ammonia loss was decreased by 5%. The lowest hydrolysis rate (65%) occurred with the boric acid treatment. The differences between the hydrolysis rates of the other treatments were not statistically significant. Phosphogypsum was found the most effective agent in reducing ammonia losses from urea. When phosphogypsum was mixed at 2.3 times as much as the urea, ammonia loss was about 85% less than that of urea applied alone. Obviously, further work is needed to find out the potential of both boric acid and phosphogypsum as reducing agents of ammonia losses from urea.
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  • 14
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    Nutrient cycling in agroecosystems 22 (1990), S. 97-107 
    ISSN: 1573-0867
    Keywords: Superphosphate ; placement depth ; banding ; relative effectivenesss ; lupins ; wheat ; field experiment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a field experiment on a sandplain soil in a low rainfall (326mm per annum) Mediterranean environment of south-western Australia, seven levels of single superphosphate, 0, 7.5, 10, 14, 19.5, 30 and 39 kg P ha−1, were placed at either 3, 5, 7, 9, 11 or 13 cm depth before sowing wheat (Triticum aestivum) at 3 cm. In a separate treatment, superphosphate was drilled with the seed (the normal practice). In the second year, the plots were sown with lupins (Lupinus angustifolius) at 3 cm depth with no additional superphosphate. In three separate treatments, superphosphate at 0, 14 and 39 kg P ha−1, was drilled with the lupin seed (the normal practice) on plots that had received no superphosphate in the first year. Yields of wheat and lupins were used as a measure of the effectiveness of the superphosphate placement treatments relative to the effectiveness of superphosphate drilled with seed of wheat (year 1) or lupins (year 2), to give relative effectiveness (RE) values in each of the two years. In the first year the RE of superphosphate was increased by about 20% when the fertilizer was placed 5 to 9 cm deep in the soil. In the second year, the RE of superphosphate for producing lupin grain was increased by about 30–60% where the fertilizer had been placed 5–13 cm deep in the previous year compared with freshly drilled 3 cm deep. The yield of wheat or lupins was closely related to the P content of plant tissue; each relationship was independent of the depth or year of superphosphate application.
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  • 15
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    Nutrient cycling in agroecosystems 26 (1990), S. 229-235 
    ISSN: 1573-0867
    Keywords: Grass ; wheat ; nitrogen nutrition ; dilution curve ; mineral content ; mineral removal ; phosphorus ; potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The important effect of nitrogen in changing the patterns of mineral content and mineral removal is analysed for grass swards and wheat. Different models are proposed; accumulated dry matter developed throughout a growing period is shown to be an excellent reference for assessing the evolution of the plant mineral content and the mineral removal the growing crop. Applications in diagnosing mineral nutrition status and optimising fertilizer use are proposed and discussed.
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  • 16
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    Antonie van Leeuwenhoek 57 (1990), S. 43-49 
    ISSN: 1572-9699
    Keywords: calcium ; conidiation ; plasma membrane ; protoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell wall-free protoplasts of P. cyclopium could regenerate a cell wall and form mycelia in liquid culture with high rates of viability. When calcium was added to the medium, protoplasts displayed biphasic accumulation with an immediate metabolism-independent adsorption phase, followed by slow metabolism-dependent uptake. Exposure of the protoplasts to Ca2+ for periods of 2 min, followed by incubation in calcium-free medium for 24 hours, was sufficient to induce conidiation with morphogenetic events parallel to those found in cultures containing calcium throughout the incubation period, and similar to those reported in cultures inoculated from conidia. The conidiation event caused by short exposure to calcium could be reversed, within 2 hours of Ca2+ addition, by a brief treatment with the specific calcium chelating agent BAPTA (100 μM), which removed 65 to 75% of the total cell calcium. The results implicate the membrane-bound calcium fraction in the process of conidiation induction.
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  • 17
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    Landscape ecology 4 (1990), S. 211-224 
    ISSN: 1572-9761
    Keywords: calcium ; forest ; insects ; land use ; landscape ecology ; soils ; succession
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Forest structure and composition influence patterns of insect outbreaks and can be explained on the Walker Branch watershed by past land use (timber harvest and agriculture), soils, aspect, and slope. In particular, pine bark beetles caused large losses of pine on sites that had been used for agriculture, on Fullerton silt loam soils, and on north-to-northeast and east-to-southeast exposures. Hickory bark beetles had a high impact on hickory biomass on Bodine soil areas that were forested in 1935 and sloped greater than 11%. Thus, prior land use can have an indirect effect on future disturbances. Because forest disturbances can affect nutrient distribution, land use can also indirectly affect nutrient availability. For example, locations of hickory bark beetle outbreaks experience a large flux of calcium from dead wood to soil because hickory accumulates large amounts of calcium in woody tissue. The research demonstrates a link between past land use, insect outbreaks, and calcium cycling.
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  • 18
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    Cellular and molecular life sciences 46 (1990), S. 1016-1017 
    ISSN: 1420-9071
    Keywords: In vitro absorption ; calcium ; wheat ; Bengal gram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The in vitro absorption of calcium from the duodenum was significantly less in a group of rats fed on a wheat diet than in a group fed a wheat and Bengal gram (70∶30) diet.
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  • 19
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    Cellular and molecular life sciences 46 (1990), S. 1175-1179 
    ISSN: 1420-9071
    Keywords: Prostacyclin ; EDRF ; $$P_{O_2 } $$ ; calcium ; vascular oxygen sensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Higher developed organisms are equipped with many central and local control mechanisms, which enable an adequate blood and oxygen supply to tissues over a wide range of demands. Global adaptive responses include changes in the circulatory and ventilatory system as well as increases in the oxygen carrying capacity of the blood. At the level of the specialized organs there exist additional control systems for the regulation of local blood flow. Most systems make use of highly specialized cells which are able to sense the oxygen partial pressure of the transport medium, blood, and within the tissues. In the past years, it has been shown that the vascular endothelium lining the entire circulatory system can actively modulate the vascular tone and platelet functions by the release of autacoids, among them prostacyclin and endothelium-derived nitric oxide (EDRF). Recent experiments demonstrate that the release of EDRF is $$P_{O_2 } $$ -dependent, which suggests that endothelial cells may act as functional local oxygen sensors within the vascular system.
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  • 20
    ISSN: 1432-1939
    Keywords: Multispecies canopy model ; Canopy photosynthesis model ; Triticum aestivum ; Avena fatua ; Ultraviolet-B radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Competition for light among species in a mixed canopy can be assessed quantitatively by a simulation model which evaluates the importance of different morphological and photosynthetic characteristics of each species. A model was developed that simulates how the foliage of all species attenuate radiation in the canopy and how much radiation is received by foliage of each species. The model can account for different kinds of foliage (leaf blades, stems, etc.) for each species. The photosynthesis and transpiration for sunlit and shaded foliage of each species is also computed for different layers in the canopy. The model is an extension of previously described single-species canopy photosynthesis simulation models. Model predictions of the fraction of foliage sunlit and interception of light by sunlit and shaded foliage for monoculture and mixed canopies of wheat (Triticum aestivum) and wild oat (Avena fatua) in the field compared very well with measured values. The model was used to calculate light interception and canopy photosynthesis for both species of wheat/wild oat mixtures grown under normal solar and enhanced ultraviolet-B (290–320 nm) radiation (UV-B) in a glasshouse experiment with no root competition. In these experiments, measurements showed that the mixtures receiving enhanced UV-B radiation had a greater proportion of the total foliage area composed of wheat compared to mixtures in the control treatments. The difference in species foliage area and its position in the canopy resulted in a calculated increase in the portion of total canopy radiation interception and photosynthesis by wheat. This, in turn, is consistent with greater canopy biomass of wheat reported in canopies irradiated with supplemental UV-B.
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  • 21
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    European journal of clinical pharmacology 38 (1990), S. 189-193 
    ISSN: 1432-1041
    Keywords: estramustine phosphate ; prostatic cancer ; gastrointestinal absorption ; food intake ; calcium ; drug interaction ; pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The effect of milk and food on the pharmacokinetics of estramustine phosphate was investigated in six patients with prostatic cancer. In a randomized three-way cross-over study, the patients were given single doses of the drug together with low calcium water, low calcium food and milk. The evaluation was based upon the plasma concentration of two metabolites, estromustine and estrone, as parent drug could not be detected in plasma. The tmax and lag time of estromustine were significantly increased by milk and food intake and Cmax and AUC were significantly decreased. In comparison with water, the AUC of estromustine was 41% when the drug was taken with milk and 67% after simultaneous intake of standardized food. Corresponding figures for the peak values were 32 and 57%, respectively. The effect of milk and food intake on the pharmacokinetics of estrone was similar. Studies in vitro demonstrated that the dissolution of estramustine phosphate disodium was markedly impaired in the presence of calcium. It was concluded that the rate and extent of absorption of estramustine phosphate were decreased when the drug was taken with milk or food due to the formation of a poorly absorbable calcium complex. To obtain high and reproducible absorption of Estracyt®, the drug should not be taken together with milk, milk products or other calcium-rich food or drugs.
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  • 22
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    Molecular and cellular biochemistry 99 (1990), S. 25-32 
    ISSN: 1573-4919
    Keywords: calcium ; regucalcin ; protein synthesis ; aminoacyl-tRNA synthetase ; rat liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effect of regucalcin, a calcium-binding protein isolated from rat liver cytosol, onin vitro protein synthesis in the 5500g supernatant fraction of rat liver homogenate was investigated. Addition of Ca2+ up to 5.0 μM in the reaction mixture caused a significant decrease in protein synthesis. This decrease was saturated at 10 μM Ca2+. The Ca2+ effect was not reversed by the presence of regucalcin (2.0 μM); the protein caused a remarkable decrease in hepatic protein synthesis, and it enhanced significantly the Ca2− effect. Meanwhile, calmodulin (2.5-20 μg/ml), a calcium-binding protein, did not have an appreciable effect on the Ca2+ (10 μM)-induced decrease in hepatic protein synthesis. [3H]Leucyl-tRNA synthetase activity in the 105000g supernatant fraction (cytosol) of liver homogenate was markedly decreased by addition of Ca2+ (1.0–50 μM). This decrease was not reversed by the presence of regucalcin (2.0 μM); the protein (1.0–2.0 μM) caused a remarkable decrease in the enzyme activity. The present results suggest that regucalcin can regulate protein synthesis in liver cells.
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  • 23
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    Molecular and cellular biochemistry 95 (1990), S. 133-137 
    ISSN: 1573-4919
    Keywords: nifedipine ; neutrophil ; calcium
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Administration of Ca2+ channel blockers in cardiac disorders and the central role of Ca 2+ in modulating neutrophil functions, prompted us to investigate whether administration of nifedipine to mice would alter their natural resistance to infectious agents like Salmonella typhimurium. Neutrophil chemiluminescence (CL) in response to S. typhimurium was significantly (p 〈 0.01) decreased in mice fed with nifedipine (0.015 mg/kg body weight) over a period of six months. Intracellular killing of S. typhimurium by isolated neutrophils also decreased significantly (p 〈 0.01) and exponentially with nifedipine administration, representing a 42% fall at six months. In addition the drug administration lowered the survival rate of animals following challenge by a lethal dose of S. typhimurium (LD50 = 1 × 104 bacteria/animal). Our data suggest that long term administration of nifedipine lowers the natural resistance of mice to S. typhimurium owing to impaired neutrophil functions.
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    Molecular and cellular biochemistry 99 (1990), S. 67-74 
    ISSN: 1573-4919
    Keywords: calcium ; lanthanide ; Europium ; sarcoplasmic reticulum ; ATP ; calcium occlusion ; tryptic digestion ; calcium-uptake
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Europium luminescence from europium bound to sarcoplasmic reticulum (Ca2+ Mg2+)-ATPase indicates that there are two high affinity calcium binding sites. Furthermore, the two calcium ions at the binding sites are highly coordinated by the protein as the number of H2O molecules surrounding the Ca2+ ions are 3 and 0.5. In the presence of ATP, calcium ions are occluded even further down to 2 and zero H2O molecules, respectively. The Ca2+ - Ca2+ intersite distance is estimated to be 8–9 Å and the average distance from the Ca2+ sites to CrATP is about 18 Å. Digestion of the (Ca2+ + Mg2+)-ATPase at the T2 site (Arg 198) causes uncoupling of Ca2+-transport from ATPase activity while calcium occlusion due to E1-P formation remains unchanged. Further tryptic digestion beyond T2 and in the presence of ATP diminishes Ca2+ occlusion to zero while 50% of the ATPase hydrolytic activity remains. Tryptic digestion beyond T2 and in the absence of ATP diminishes ATPase hydrolytic activity to 50% of normal while Ca2+ occlusion remains intact. These data are consistent with a mechanism in which the functional enzyme must be in the dimeric form for occlusion and calcium uptake to occur, but each monomer can hydrolyze ATP.
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  • 25
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    Molecular and cellular biochemistry 93 (1990), S. 27-34 
    ISSN: 1573-4919
    Keywords: neutrophil ; superoxide ; calcium ; nifedipine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Superoxide production by mice neutrophils was inhibited by nifedipine exposure in a dose dependent manner. The inhibition of Ca2+ uptake elicited by nifedipine did not appear to account for the observed effect as the extracellular Ca2+ enrichment and depletion did not produce a significant reversal of the inhibition. Cytosolic free Ca2+ as measured by Quin 2AM fluorescence did not show any significant change, indicating that the effect was independent of the inhibition of Ca2+ influx. In addition nifedipine caused a significant inhibition (p 〈 0.01) in NADPH oxidase activity. Our data indicates that nifedipine inhibits superoxide production independent of inhibiting Ca2+ inflow and supports the hypothesis that Ca2+ antagonists affect cellular functions by non Ca2+ mediated process as well.
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  • 26
    ISSN: 1573-4919
    Keywords: myosin ; calcium ; calmodulin ; type II kinase
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Brain type II Ca2+/calmodulin-dependent protein kinase was found to phoshorylate smooth muscle myosin, incorporating maximally ∼ 2 mol of phosphoryl per mol of myosin, exclusively on the 20,000 dalton light chain subunit. After maximal phosphorylation of myosin or the isolated 20,000 dalton light chain subunit by myosin light chain kinase, the addition of type II Ca2+/calmodulin-dependent protein kinase led to no further incorporation indicating the two kinases phosphorylated a common site. This conclusion was supported by two dimensional mapping of tryptic digests of myosin phosphorylated by the two kinases. By phosphoamino acid analysis the phosphorylated residue was identified as a serine. The phosphorylation by type II Ca 2+/calmodulin-dependent protein kinase of myosin resulted in enhancement of its actin-activated Mg2+-ATPase activity. Taken together, these data strongly support the conclusion that type II Ca2+/calmodulin-dependent protein kinase phosphorylates the same amino acid residue on the 20,000 dalton light chain subunit of smooth muscle myosin as is phosphorylated by myosin light chain kinase and suggest an alternative mechanism for the regulation of actin-myosin interaction.
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  • 27
    ISSN: 1573-1561
    Keywords: Allelochemicals ; no-tillage ; conventional-tillage ; soils ; wheat ; Triticum aestivum ; mass spectrometry ; Petri-dish bioassay ; fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Putative allelochemicals found in the soil of no-tillage and conventional-tillage wheat plots near Stillwater, Oklahoma, were obtained by a mild alkaline aqueous extraction procedure, bioassayed to determine their biological activity, purified, and analyzed with a capillary gas chromatography-mass spectrometry-data analysis system. The most significant inhibition was found in bioassays of extracts from soil collected immediately after harvest in June, July, and August. No-tillage soils produced significant inhibition during the rest of the year also. Mass spectrometry showed fatty acids as the most abundant compounds. However, when bioassayed authentic samples of the five free fatty acids showed no significant biological activity toward wheat.
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  • 28
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    Journal of chemical ecology 16 (1990), S. 1927-1940 
    ISSN: 1573-1561
    Keywords: Allelopathy ; phytotoxic ; allelochemical ; α-bisabolol ; δ-cadinene ; p-cymen-9-ol ; essential oil ; germination ; phenethyl alcohol ; piperitenone ; vanillin ; Gossypium hirsutum ; Proboscidea louisianica ; Triticum aestivum
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The potential allelopathic activity of devil's-claw [Proboscidea louisianica (Mill.) Thellung] essential oil and a few of the compounds it contains on the elongation of cotton (Gossypium hirsutum L.) and wheat (Triticum aestivum L.) radicles was studied using a Petri dish bioassay. Essential oil was collected by steam distillation using an all-glass-Teflon assembly. Ether extracts of the steam distillates from fresh devil's-claw were inhibitory to cotton and wheat radicle elongation. The following six components of devil's-claw essential oil identified by CGC-MS-DS were inhibitory to cotton and/or wheat at a concentration of 1 mM: vanillin, piperitenone, δ-cadinene,p-cymen-9-ol, α-bisabolol, and phenethyl alcohol.
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  • 29
    ISSN: 1573-4978
    Keywords: calcium ; cDNA clone ; epidermal differentiation ; keratinocyte ; retinoic acid
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    Topics: Biology
    Notes: Abstract Differential screening of a human epidermal cDNA library led to the isolation of cDNA clones homologous to mRNAs specifically expressed in epidermis but weakly or not expressed in the undifferentiated squamous carcinoma cell line TR146. One of these ‘differentiation-specific’ cDNA clones, A8, hybridized with a 1.7 kb transcript among RNAs isolated from normal human epidermis, but with several transcripts ranging from 1.4 to 2.1 kb when mRNAs were isolated from cultured keratinocytes. We examined the effects of modulators of epidermal differentiation such as calcium and retinoic acid on the production of these transcripts. Their amount was found to increase in the presence of high calcium concentration, but to decrease in the presence of retinoic acid. These results strongly suggest that A8 messages are up-regulated during epidermal differentiation. The sequence of the 1371 bp of A8 cDNA shows a very high GC content. Because of its homology with the murine loricrin mRNA, A8 is likely to correspond either to the human loricrin or to a related protein.
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  • 30
    ISSN: 1573-4935
    Keywords: myelin basic protein ; lymphocytes ; phytohaemagglutinin ; calcium
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Myelin Basic Protein, one of the major membrane protein component of the central nervous system, was used to probe the molecular mechanism of cellular activation by phytohaemagglutinin. Pre-treatment of human lymphocytes with myelin basic protein results in a lower rising of cytosolic concentration of free calcium after stimulation with phytohaemagglutinin. This effect is dependent on myelin basic protein concentration and on the preincubation time of the protein with the cells. It is not due to a interaction between myelin basic protein and phytohaemagglutinin, but appears to be a consequence of the binding of the protein to the cell surface. The reduction of the rise of cytosolic calcium induced by phytohaemagglutinin is specific for the myelin basic protein because other proteins like albumin and protamine have no effect.
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  • 31
    ISSN: 1573-5028
    Keywords: tRNA-like sequences ; t-elements ; RNA processing ; mitochondria ; wheat
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    Topics: Biology
    Notes: Abstract We have recently described the properties of a wheat mitochondrial extract that is able to process, accurately and efficiently, artificial transcripts containing wheat mitochondrial tRNA sequences, with the production of mature tRNAs (P.J. Hanic-Joyce and M.W. Gray, J. Biol. Chem., in press). Such processing involves 5′-endonucleolytic, 3′-endonucleolytic, and TRNA nucleotidyltransferase activities. Here we show that this system also acts on transcripts containing sequences corresponding to an unusual class of short repeats (‘t-elements’) in wheat mtDNA. These repeats are theoretically capable of assuming a tRNA-like secondary structure, although stable transcripts corresponding to them are not detectable in vivo. We find that t-element sequences are processed with the same specificity and with comparable efficiency as are authentic tRNA sequences. Because known t-elements are located close to and in the same transcriptional orientation as active genes (18S-5S, 26S, tRNAPro) in wheat mtDNA, our results raise the question of whether t-elements play a role in gene expression in wheat mitochondria.
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  • 32
    ISSN: 1573-5028
    Keywords: α-amylase tetrameric inhibitor ; cDNA cloning ; genetic mapping ; wheat
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    Notes: Abstract We have characterized three cDNA clones corresponding to proteins CM1, CM3 and CM16, which represent the three types of subunits of the wheat tetrameric inhibitor of insect α-amylases. The deduced amino acid sequences of the mature polypeptides are homologous to those of the dimeric and monomeric α-amylase inhibitors and of the trypsin inhibitors. The mature polypeptides are preceded by typical signal peptides. Southern blot analysis of appropriate aneuploids, using the cloned cDNAs as probes, has revealed the location of genes for subunits of the CM3 and of the CM16 type within a few kb of each other in chromosomes 4A, 4B and 4D, and those for the CM1 type of subunit in chromosomes 7A, 7B and 7D. Known subunits of the tetrameric inhibitor corresponding to genes from the B and D genomes have been previously characterized. No proteins of this class have been found to be encoded by the A genome in hexaploid wheat (genomes AA, BB, DD) or in diploid wheats (AA) and no anti α-amylase activity has been detected in the latter, so that the A-genome genes must be either silent (pseudogenes) or expressed at a much lower level.
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  • 33
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    Plant molecular biology 15 (1990), S. 793-795 
    ISSN: 1573-5028
    Keywords: mitochondrial gene ; ORF25 ; wheat
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    Topics: Biology
    Notes: Abstract The wheat mitochondrial orf25 nucleotide sequence of 576 pb has been determined. Its derived protein sequence shares 88% and 75% amino acid identity with those of maize and tobacco mitochondria, respectively. The wheat and tobacco orf25 sequences lack four inserts, of 6 bp to 36 bp, that are present in the maize homologue. The wheat orf25 gene is actively transcribed and is preceded by a regulatory sequence block very similar to those located upstream of the wheat coxII and atp6 genes. Our observations support the view that orf25 sequences encode a functional polypeptide in plant mitochondria.
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  • 34
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    Plant molecular biology 15 (1990), S. 947-950 
    ISSN: 1573-5028
    Keywords: clpP gene ; rps12 gene ; ribosomal protein S12 ; intron ; Triticum aestivum
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  • 35
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    Plant cell reports 9 (1990), S. 14-16 
    ISSN: 1432-203X
    Keywords: Triticum aestivum ; Anther culture ; Sugars ; Wheat
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    Topics: Biology
    Notes: Summary The effect of employing different sugars in wheat anther culture has been investigated using four Spring wheat cultivars. The most responsive cultivar, Orofen, gave a three to four-fold increase in embryo yield when maltose was used in place of sucrose, with 50 embryos being produced for every 100 anthers cultured. Measurement of sugar concentrations in the culture media indicated that sucrose was more rapidly hydrolysed than maltose. However, neither the osmotic potential of the medium nor the concentration of glucose appeared to be critical factors in determining embryo yield.
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  • 36
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    The journal of membrane biology 118 (1990), S. 69-75 
    ISSN: 1432-1424
    Keywords: Cl− channels ; platelet ; calcium ; patch clamp
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Whole-cell patch-clamp recordings were made from freshly isolated human platelets. The pipette contained a high concentration of divalent cations, which permitted easy disruption of cell-attached membrane patches by suction. Single-channel currents were measured when the pipette contained isotonic BaCl2 or MgCl2 saline; over 30 sec −5 min an increasing number of channels appeared until conductance steps through individual channels could no longer be distinguished. The current-voltage relationship was curvilinear; chord conductance at −35 mV was 25 pS increasing to 45 to 52 pS at +45 mV. Ion substitution experiments showed the current to be primarily carried by Cl−.E rev was shifted 30 mV/10-fold change in external Cl− (replaced by gluconate), was similar with BaCl2 or MgCl2 in the pipette and was not significantly shifted by replacing external Na+ with K+. Addition of 1mm BAPTA to the MgCl2 pipette saline prevented activation of Cl− currents; with isotonic CaCl2 internal saline, current appeared immediately upon patch rupture, suggesting that the Cl− channels are dependent on internal Ca2+, 5-nitro-2-(3-phenylpropylamino)-benzoate, reported to block a Cl− conductance in studies of rat epithelial cells, caused a potent flickery block and may be a useful tool with which to investigate the physiological role of Cl− currents in human platelets.
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    The journal of membrane biology 116 (1990), S. 1-8 
    ISSN: 1432-1424
    Keywords: transport ; calcium ; ATPase ; binding ; phosphorylation ; catalysis
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  • 38
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    The journal of membrane biology 113 (1990), S. 177-191 
    ISSN: 1432-1424
    Keywords: membrane transport ; electrogenic carrier ; calcium ; membrane potential ; sodium-calcium exchange
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  • 39
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    The journal of membrane biology 114 (1990), S. 189-194 
    ISSN: 1432-1424
    Keywords: algae ; calcium ; ion transport ; plant cell ; turgor pressure ; turgor regulation
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  • 40
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    The journal of membrane biology 116 (1990), S. 239-248 
    ISSN: 1432-1424
    Keywords: gastric glands ; fura-2 ; calcium ; sodium ; pH
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The dependence of cytoplasmic free [Ca] (Ca i ) on [Na] and pH was assessed in individual parietal cells of intact rabbit gastric glands by microfluorimetry of fura-2. Lowering extracellular [Na] (Na o ) to 20mm or below caused a biphasic Ca i increase which consisted of both release of intracellular Ca stores and Ca entry across the plasma membrane. The Ca increase was not blocked by antagonists of Ca-mobilizing receptors (atropine or cimetidine) and was independent of the replacement cation. Experiments in Ca-free media and in Na-depleted cells indicated that neither phase was due to reversal of Na/Ca exchange. The steep dependence of the Ca i increase on Na o suggested that the response was not due to lowering intracellular [Na] (Na i ). The effects of low Na o on Ca i were also completely independent of changes in intracellular pH (pH i ). Ca i was remarkably stable during changes of pH i of up to 2 pH units, indicating that H and Ca do not share a cytoplasmic buffer system. Such large pH excursions required determination of the pH dependence of fura-2. Because fura-2 was found to decrease its affinity for Ca as pH decreased below 6.7, corrections were applied to experiments in which large pH i changes were observed. In contrast to the relative insensitivity of Ca i to changes in pH i , decreasing extracellular pH (pH o ) to 6.0 or below was found to stimulate release of intracellular Ca stores. Increased Ca entry was not observed in this case. The ability of decreases in Na o and pH o to stimulate release of intracellular Ca stores suggest interactions between Na and H with extracellular receptors.
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  • 41
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    Theoretical and applied genetics 79 (1990), S. 401-410 
    ISSN: 1432-2242
    Keywords: Puccinia graminis tritici ; Puccinia recondita Tritici ; Triticum aestivum ; Rust resistance ; Gene identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genes for resistance toPuccinia graminis tritici andPuccinia recondita tritici identified in four South African wheats were:Sr6,Sr8a,Sr9e, andLr13 in ‘W3762’;Sr5,Sr8a,Sr9b,Sr12,Sr24,Lr13, andLr24 in ‘W3760’;Sr2,Sr24,SrC,Lr13, andLr24 in ‘W3751’; andSr7a,Sr23,Sr36, andLr16 in ‘W3755’. GenesSr2,Sr9e, andSr24 also conferred adult plant resistance to the predominant pathotypes ofP. graminis tritici. GenesSr7a,Sr23, andSrC, when present alone, did not confer acceptable adult plant resistance, even though low seedling reactions were associated with them when tested with the same pathotypes. Genetic recombination betweenLr13 andSr9e was estimated at 12.5%±2.3%.
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  • 42
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    Theoretical and applied genetics 79 (1990), S. 77-80 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Anther culture ; 1B/1R translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The in vitro microspore androgenesis reaction of 25 commercial German spring (including 4 Triticum durum) and 50 winter wheat cultivars was investigated. Tremendous genotypical differences were found in microspore response. The best-responding winter wheat cultivai, “Florida”, is characterized by the presence of a 1B/1R wheat-rye translocation chromosome. The significance of this finding and other genetic systems for future use of haploids in plant breeding is discussed.
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  • 43
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    Theoretical and applied genetics 79 (1990), S. 305-313 
    ISSN: 1432-2242
    Keywords: Peroxidase ; Isoelectric focusing ; Hexaploid ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Isoelectric focusing (IEF) of extracts from different tissues of hexaploid wheat cv “Chinese Spring” provided a method of distinguishing and identifying the four known, and one newly discovered, sets of genes encoding peroxidase isozyme production.Per-1, carried on the short arms of homoeologous group 1 chromosomes, shows a high degree of conservation and is active in coleoptile tissue.Per-2, carried on the short arms of group 2 chromosomes, shows some polymorphism and is most active in root tissue.Per-3, on the long arms of group 3 chromosomes, is highly variable and most active in embryo tissue.Per-4, carried on chromosome arms7AS,4AL, and7DS, is quite variable and most active in endosperm tissue. (The chromosome nomenclature used in this paper is that agreed to by the 7th International Wheat Genetics Symposium, where the previous designations of4A and4B were reversed.) Restriction fragment length polymorphism (RFLP)-based maps of the group 7 chromosomes were used to locatePer-A4 to a distal region of7AS. In addition, a further set of genes was identified as being active in root tissue. In wheat a single locus,Per-D5, was found on chromosome arm2DS.
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  • 44
    ISSN: 1432-2242
    Keywords: Cereals ; Gramineae ; Somatic embryogenesis ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Immature embryos, inflorescences, and anthers of eight commercial cultivars of Triticum aestivum (wheat) formed embryogenic callus on a variety of media. Immature embryos (1.0–1.5 mm long) were found to be most suitable for embryogenic callus formation while anthers responded poorly; inflorescences gave intermediate values. Immature embryos of various cultivars showed significant differences in callus formation in response to 11 of the 12 media tested. No significant differences were observed when the embryos were cultred under similar conditions on MS medium with twice the concentration of inorganic salts, supplemented with 2,4-D, casein hydrolysate and glutamine. Furthermore, with inflorescences also no significant differences were observed. Explants on callus formation media formed two types of embryogenic calli: an off-white, compact, and nodular callus and a white compact callus. Upon successive subcultures (approximately 5 months), the nodular embryogenic callus became more prominent and was identified as ‘aged callus’. The aged callus upon further subculture, formed an off-white, soft, and friable embryogenic callus. Both the aged and friable calli maintained their embryogenic capacity over many subculture passages (to date up to 19 months). All embryogenic calli (1 month old) from the different callus-forming media, irrespective of expiant source, formed only green shoots on regeneration media that developed to maturity in the greenhouse. There were no significant differences in the response of calli derived from embryos and inflorescences cultured on the different initiation media. Also, the shoot-forming capacity of the cultivars was not significantly different. Anther-derived calli formed the least shoots. Aged and friable calli on regeneration media also formed green shoots but at lower frequencies. Plants from long-term culture have also been grown to maturity in soil.
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  • 45
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    Theoretical and applied genetics 80 (1990), S. 359-365 
    ISSN: 1432-2242
    Keywords: Helminthosporium ; Fusarium ; Phytophthora ; Hordeum vulgare ; Triticum aestivum ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Because plant cells cultured in vitro express genetic variability and since they can be regenerated into functional plants, procedures have been designed to use this system for the production of plants with new important agronomic characteristics, particularly for disease resistance. For barley, wheat, and potato somaclones have been found that were less susceptible to a toxin of Helminthosporium, fusaric acid, Fusarium coeruleum, F. sulphureum, or Phytophthora infestans, when screened in the first in-vitro-derived generation. Here the progeny of such somaclones is evaluated after natural and artificial infection, using greenhouse-grown or field material. The progenies of the same somaclones did not express detectable differences, which indicated that no heterozygous mutations occurred. Most lines and clones differed in their level of susceptibility to the pathogen compared to the level of the starting material, but these data were in no instance significant. It is discussed here whether this lack of significance is due to a lack of genetic differences or whether the test procedures are in adequate for detecting and securing the slight, probably quantitative, alterations.
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  • 46
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Glutenin ; Gliadin ; Aneuploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The endosperm storage proteins, glutenin and gliadin, are major determinants of bread-making quality in hexaploid wheat. Genes encoding them are located on chromosomes of homoeologous groups 1 and 6. Aneuploid lines of these groups in spring wheat cultivar ‘Chinese Spring’ have been used to investigate the effect of varying the dosage of chromosomes and chromosome arms upon bread-making quality, where quality has been assessed using the SDS-sedimentation test. Differences between the group 1 chromosomes for quality were greater than those between the group 6 chromosomes. The chromosomes were ranked within homoeologous groups for their effect on quality as follows (〉=better quality): 1D〉1B〉1A and 6A〉6B=6D. The relationship of chromosome dosage with quality was principally linear for four of the chromosomes, but not for 6B and 6D. Increases in the dosage of 1B, 6A and, especially, 1D, were associated with significant improvements in quality, whereas increases in the dosage of 1A were associated with reductions in quality. The effects of 1A and 1D were such that the best genotype for quality was nullisomic 1A-tetrasomic 1D. For group 1, effects of the long arm appeared in general to be more important than effects of the short arm. For group 6, effects were found associated with the long arms as well as with the short arms, a surprising result in view of the absence of genes encoding storage proteins on the long arms. Significant interactions were found between chromosomes and genetic backgrounds, and between individual chromosomes. Analysis of trials grown over two years demonstrated that, although additive environmental differences over years and genotype x years interaction were present, they were relatively small in magnitude compared with purely genetic differences.
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  • 47
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    Protoplasma 153 (1990), S. 193-203 
    ISSN: 1615-6102
    Keywords: Endosperm ; Intercellular protoplasmic movement ; Plasmodesma ; Nuclear migration ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Earlier work in our laboratory indicated that protoplasmic constituents can migrate from one cell to another in certain tissues of higher plants. Further investigations have been conducted using garlic bulbs and wheat nucellus for microscopic observation of intercellular protoplasmic movement in vivo. These gave preliminary indications of the dynamic characteristics of migrating nuclei and cytoplasm. The present paper gives recent results providing new evidence for intercellular protoplasmic movement that is neither hindered by the presence of cell wall nor the narrowness of channels of intercellular connection. By careful manipulation, intact endosperm sacs could be taken from developing caryopses (6–8 days after fertilization) without apparent injury to constituent cells. Shortly after the living specimen is mounted on the microscopic stage, asynchronous intercellular protoplasmic movement can be observed here and there. It can be seen that protoplasm extrudes in rapid but intermittent movements from one cell to the next by vigorous contraction. Although various cell constituents may move together, they can also be quite independent of each other. The moving units, though undergoing violent deformation, resume their normal shape and structure following intercellular migration. Evidently this kind of movement is a naturally occurring and active phenomenon closely related to the physiological state of the tissue. Electron microscopic studies reveal that a limited number of plasmodesmatal channels undergo modification and enlarge to 100–400 nm, through which the protoplasmic constituents pass.
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    Bulletin of experimental biology and medicine 109 (1990), S. 50-52 
    ISSN: 1573-8221
    Keywords: HeLa ; histamine ; calcium ; phorbol esters
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    Topics: Biology , Medicine
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  • 49
    ISSN: 1573-8469
    Keywords: yellow (stripe) rust races ; Triticum aestivum ; Yr genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Onder geconditioneerde klimaatsomstandigheden zijn 42 Ethiopische en CIMMYT rassen en lijnen van broodtarwe (Triticum aestivum) in het kiemplantstadium geïnoculeerd met 19 isolaten van gele roest die onderling verschilden in hun pathogeniteit voor 20 differentiërende tarwerassen waarvan de resistantie-achtergrond bekend is. De genom-gen relatie is toegepast om resistentiegenen te identificeren. Vier rassen en lijnen bleken resistent te zijn tegen alle isolaten. Verondersteld wordt dat hun resistentie berust op genen die niet eerder herkend waren of op een combinatie van bekende genen die niet compatibel was met de gebruikte isolaten. In het overige tarwemateriaal kon de aanwezigheid worden aangegeven van de resistentiegenenYr2, Yr3, Yr4, Yr6, Yr7, Yr9 enYrA. Het van rogge afkomstige en door het CIMMYT veel gebruikte resistentiegenYr9 was in 28 rassen en lijnen (67%) aanwezig. In het onderzochte tarwemateriaal isYr4 het enige voor Oost en Centraal Afrika effectieve resistentiegen omdat de daar voorkomende gele roest pathogeniteit bezit voor de overige genen. Het herkennen van pathogeniteit van gele roest voor bepaalde resistentiegenen is verbeterd door het toevoegen van tarwerassen met monogene resistentie aan het internatinale gebruikte tarwesortiment voor de determinatie van gele-roestfysio's.
    Notes: Abstract In a controlled environment, the reaction was observed of 42 bread wheat varieties and lines inoculated with 19 isolates of yellow rust differing in their virulence to 20 differential varieties. Five varieties and lines showed resistance to all isolates. The remaining ones appeared to have the genesYr2, Yr3, Yr4, Yr6, Yr7, Yr9 andYrA, either singly or in combination.Yr9 derived from rye was present in 67% of the varieties and lines.Yr4 is the only effective gene in that material as, in Eastern and Central Africa, yellow rust has virulence to the otherYr genes. Recognition of virulence toYr genes is enhanced by the use of a supplemental set of differential varieties supposedly carrying a single gene.
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  • 50
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    Bioscience reports 10 (1990), S. 93-103 
    ISSN: 1573-4935
    Keywords: exocytosis ; calcium ; G protein ; fura-2 ; patch-clamp ; capacitance ; neutrophil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Exocytosis and intracellular free calcium ([Ca2+]in) were simultaneously recorded in single human neutrophils using patch-clamp capacitance measurements and the fura-2 fluorescence ratio method. Intracellular application of guanosine-5′-O(3-thiotriphosphate) (GTPγS) stimulates both exocytosis and a calcium transient. The calcium transient starts to develop after a lag phase of ∼40s and normally appears to trigger the onset of exocytosis indicated by the beginning of the capacitance increase. After this delay [Ca2+]in increases from ∼150 nM to ∼600 nM with a sigmoidal time course. The peak concentration is reached within ∼30 s but the main increase occurs during ∼ 3s. [Ca2+]in subsequently decays within 1–2 min to a level which is close to the resting value. This calcium transient is due to calcium release from inositoltrisphosphate-sensitive intracellular stores. Exocytosis also occurs if the calcium transient is abolished by intracellular EGTA but the lag phase is markedly prolonged. The GTPγS-induced calcium transient is very similar to that observed after stimulation with N-formyl-methionyl-leucyl-phenylalanine. The interplay between guanine nucleotides, [Ca2+]in and exocytosis in neutrophils closely resembles previous results obtained in mast cells suggesting a similar regulation of exocytosis in both cell types.
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  • 51
    ISSN: 1573-4935
    Keywords: vasoactive intestinal peptide (VIP) ; thyrotropin releasing hormone (TRH) ; prolactin (PRL) ; lactotrophs ; anterior pituitary ; second messengers ; hormone secretion ; adenylate cyclase ; calcium ; inositol trisphosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effect of vasoactive intestinal peptide (VIP) on prolactin (PRL) secretion from pituitary cells is reviewed and compared to the effect of thyrotropin releasing hormone (TRH). These two peptides induced different secretion profiles from parafused lactotrophs in culture. TRH was found to increase PRL secretion within 4 s and induced a biphasic secretion pattern, while VIP induced a monophasic secretion pattern after a lag time of 45–60 s. The secretion profiles are compared to changes in adenylate cyclase activity, production of inositol polyphosphates, changes in intracellular calcium concentrations and changes in electrophysiological properties of the cell membrane.
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  • 52
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    Bioscience reports 10 (1990), S. 493-507 
    ISSN: 1573-4935
    Keywords: osteoclast ; intracellular ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract There is a growing list of cells that are capable of detecting and responding to changes in the concentration of extracellular calcium. The two classic examples of this behaviour are the calcitonin-secreting parafollicular cells of the thyroid and parathyroid hormone-secreting chief cells of the parathyroid gland. A more recent addition to this list is the renin-secreting juxtaglomerular cell of the kidney. Particularly intriguing has been independently the discovery by two laboratories, that the resorptive cell of bone, the osteoclast, is capable of detecting changes in ambient calcium. A common theme amongst all these so called “calcium-responsive” cells is that extracellular calcium increases elevate intracellular calcium levels, and this intracellular signal is either stimulatory or inhibitory to the functional response. But how these cells detect changes in the concentration of extracellular calcium, and how these recognition events are subsequently transformed into intracellular signals that regulate cell function are somewhat less clear. The commentary reveals some recent developments that seemingly provide insights into these mechanisms, with special reference to the osteoclast.
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  • 53
    ISSN: 1573-4935
    Keywords: transglutaminase ; calcium ; insulin release
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ca2+-Induced insulin release from electropermeabilised islets is inhibited by the transglutaminase inhibitors monodansylcadaverine, glycine methylester, methylamine and cystamine but not by the control compounds dimethyl monodansylcadaverine and sarcosine methylester which lack the primary amine group. Neither monodansylcadaverine nor glycine methylester inhibited insulin secretion induced by either cAMP or the phorbol ester PMA at basal levels (10 nM) of Ca2+. These data provide further evidence for the involvement of transglutaminase in Ca2+ induced insulin secretion, they also suggest that insulin secretion induced by either cAMP or PMA may act in part by a mechanism independent of that induced by Ca2+.
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  • 54
    ISSN: 1573-5036
    Keywords: Lycopersicon esculentum L. ; magnesium-manganese interaction ; magnesium: manganese ratio ; manganese toxicity ; tomato ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Results are reported for tomato (Lycopersicon esculentum L. var. Ailsa craig) and wheat (Triticum aestivum L. cv. Mara) which demonstrate that increasing concentrations of Mg in the plant raises plant tolerance to Mn toxicity. Water culture experiments with tomato show that under conditions of high Mn supply (200 µM, Mn), not only does increasing Mg application (0.75 mM to 15 mM) depress Mn uptake, but the higher Mg concentrations in the shoot counteract the onset of Mn toxicity when the concentrations of Mn in the shoot are also high. The ratio of Mg: Mn in the tissues is a better indicator of the appearance of toxicity symptoms than Mn concentration alone. Toxicity symptoms were observed when the Mg:Mn ratio in the shoot tissue was from 1.13 to a value between 3.53 and 6.54. The corresponding Mg: Mn ratio in the older leaves was from 0.82 to between 2.27 and 3.51. For wheat grown in soil, analyses of leaves revealed that growth could be expressed by the following relationship: Y=A+B exp(-kX), where Y=growth, X=Mg:Mn ratio, A, B and k=constants. Growth was significantly reduced when the Mg:Mn ratio fell below 20:1. From a measurement of this ratio it is therefore possible to predict the appearance of Mn toxicity and its influence on growth.
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  • 55
    ISSN: 1573-5036
    Keywords: calcium ; ear development ; magnesium ; potassium ; tissue press sap ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract For floret development and final grain number ear elongation in wheat (10–17d before anthesis) is the most sensitive period to adverse growth conditions. Sugars are supposedly the main factor determining floret numbers and grain set, and play next to potassium a significant role in establishing turgor pressure in young tissues. In view of this osmotic function, the influence of K on the concentration of water soluble carbohydrates (WSC, total of sucrose and reducing sugars), Ca and Mg was investigated in pot experiments. Further, the osmotic potential of sap extracted from young ears was determined and compared to sap extracted from the leaf blade. Plants supplied with low amounts of K (moderate K-deficiency) had a considerably lower K concentration in the press sap of the flag leaf and the ear than plants well supplied with K. Concentrations of WSC, Ca and Mg were higher in press sap of the flag leaf in K deficient plants than in plants adequately supplied with K. This indicates a substitution of K in its osmotic role. In press sap from ears, however, WSC, Ca and Mg were not influenced by the K application. Therefore, substances other than those measured must have been responsible for the osmoregulation in the young ear. WSC and the osmotic potential increased (more negative) independently on K supply during ear elongation, while K, Ca and Mg concentrations decreased. Whereas grain number was not influenced by the treatment, single grain weight at maturity was reduced by low K availability in the soil.
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  • 56
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    Plant and soil 124 (1990), S. 303-307 
    ISSN: 1573-5036
    Keywords: models ; potassium uptake ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spring wheat was grown in the field under deficient and sufficient levels of soil K and with high and low supplies of fertiliser nitrogen. Measurements were made of K uptake, soil nutrient supply parameters, root growth and, in solution culture, root influx parameters. Mechanistic models predicted uptake reasonably well under K-deficient conditions, but over-predicted uptake, by as much as 4 times, under K-sufficient conditions. The over-prediction was apparently due to poor characterisation of plant demand.
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  • 57
    ISSN: 1573-5036
    Keywords: Cu levels ; N sources ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A pot experiment was conducted, in a greenhouse, at Hisar, India, using a sandy soil deficient in nitrogen and copper, to study the effects of various levels of N and Cu on the dry matter yield and the N and Cu contents of wheat (Triticum aestivum L.). The sources of nitrogen used were Ca(NO3)2, NH4Cl and NH4NO3 applied in amounts necessary to establish 120 ppm of soil nitrogen and using a control (0 ppm N). Copper was applied, as copper chloride, to give soil Cu levels of 0, 5, 10 and 20 ppm. In general, dry matter yields, N and Cu concentrations in shoots and roots and available soil-N after harvest of the plants, followed the order Ca(NO3)〉NH4NO3〉NH4Cl. Up to a level of 5 ppm Cu, the dry matter yields of shoots and roots increased, but decreased at higher levels of Cu. Increasing Cu levels significantly decreased the available soil-nitrogen after harvest and also the concentration of N in the plants. At the same time the concentration of Cu in shoots and roots and available Cu in the soil was increased. Nitrogen and copper were found to have a mutually antagonistic effect on each other's concentration in the plants. The antagonism was greater with NH4 + sources than with NO3 − compounds.
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  • 58
    ISSN: 1573-5036
    Keywords: chelation ; EGTA ; calcium ; Medicago sativa L. ; nitrogen fixation ; Rhizobium meliloti ; rhizotron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of calcium on the nodulation of lucerne was studied using EGTA, a specific calcium-chelator. First, the effects of the chelator were tested on hydroponically grown plants at pH 7.0. Optimal numbers of nodules were obtained in nutrient solution containing 0.2 mM CaCl2. When 0.4 mM EGTA was given additionally, nodulation was completely inhibited. Nodulation was restored specifically with CaCl2, but not with MgCl2. For studies in an acid soil (pH-H2O 5.2), lucerne seedlings were grown in rhizotrons. 67% of the seedlings became nodulated when the soil around the seed was neutralized locally with 1.0 μmol of K2CO3 in drops of 12 μL volume. When native calcium was removed with 2 μmol of EGTA, nodulation was reduced to 12%. However, addition of EGTA to soil resulted in a drop of pH from 6.1 to 5.2. A phosphate buffer could also not keep soil-pH sufficiently stable. Such pH-decreases could be avoided by placing agar blocks containing 6 μmol of EGTA for three hours on freshly developed roots. This treatment reduced nodulation from 87% to 32%, with soil-pH lowering only from 6.2 to 6.0. Nodulation could be restored by adding 2 μmol of CaCl2. The depletion of soil-calcium could depress nodule formation only during the first day after inoculation.
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  • 59
    ISSN: 1573-8469
    Keywords: Puccinia recondita ; Triticum aestivum ; Photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting De relatie stussen de epidemiologie van bruine roest, bladveroudering en oprengstderving werden bestudeerd bij zeven wintertarwegenotypen die verschilden in partiële resistentie tegen bruine roest. Bruine roest versnelde de veroudering van blad en aar. Fotosynthesemetingen wezen echter uit dat de fotosynthesecapaciteit van het resterende groene oppervlak niet werd beïnvloed. Verschillen tussen de genotypen in opbrengstderving werden voornamelijk veroorzaakt door verschillen in bladveroudering en daarmee in cumulatieve lichtinterceptie (r=0.83) en gewasfotosynthese. De reductie in cumulatieve lichtinterceptie was nauw gerelateerd aan de cumulatieve ziektedruk (in puistdagen) (r=0.85), welke ook nauw was geassocieerd met de opbrengstderving (r=0.88). De opbrengstreductie werd echter even goed voorspeld door de tijdsduur tot 50% reductie van het groene bladoppervlak en de puistdichtheid halverwege de epidemie als door cumulatieve lichtinterceptie en cumulatieve ziektedruk, waardoor beide eerste criteria gebruikt kunnen worden in de selectie voor partiële resistentie tegen bruine roest.
    Notes: Abstract Relations between leaf rust progress, foliage senescence and yield reduction were studied for seven winter wheat genotypes, differing in their partial resistance to leaf rust. Leaf rust accelerated leaf and ear senescence. Photosynthesis measurements showed, however, that the photosynthetic capacity of the remaining green surface was not affected. Differences between genotypes in yield reduction were largely explained by differences in leaf senescence and, therefore, in cumulative light interception (r=0.83) and crop photosynthesis. Reduction in cumulative light interception was closely related to the area under the disease-progress-curve (r=0.85), which was also closely associated with yield reduction (r=0.88). However, the time taken to reach a 50% reduction of green leaf area and the pustule density on 1 July (i.e. halfway through the epidemic) were just as good predictors of yield reduction as cumulative light interception and area under disease-progress-curve, suggesting that the former are useful criteria in the selection for partial resistance to leaf rust.
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  • 60
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    European journal of plant pathology 96 (1990), S. 29-34 
    ISSN: 1573-8469
    Keywords: Triticum aestivum ; simulation ; SUCROS87 ; NWHEAT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Om systemen voor geleide bestrijding van ziekten te verbeteren, werd de vraag gesteld of schaderelaties, vastgesteld in jaren met specifieke weersomstandigheden, ook gebruikt kunnen worden in andere jaren. Twee bestaande modellen van tarwe en een combinatie van beide, werden gebruikt om het effect van weer op opbrengst te berekenen, eerst bij afwezigheid van meeldauw. De berekende opbrengsten in verschillende jaren toonden geen samenhang met de behaalde opbrengsten, gecorrigeerd voor een jaarlijkse toename, in Nederland. Verschillen tussen de modellen konden worden toegeschreven aan de wijze waarop de groei en ontwikkeling van tarwe vroeg in het seizoen wordt berekend.
    Notes: Abstract A vital question to upgrade disease management systems is whether damage functions, established in years with specific weather conditions, can be applied also in other years. A simulation approach was used to explore effects of weather on yield and damage. Two existing models of wheat and a third, a combination of both, were used to compute yield, first in absence of mildew. In a second paper, effects of mildew will be reported. Yields simulated for different years were not significantly correlated with yields harvested, adjusted for their increase over years, in the Netherlands. Differences in performance between the models could be attributed to the method of simulating development and growth early in the cropping season.
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  • 61
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    European journal of plant pathology 96 (1990), S. 187-198 
    ISSN: 1573-8469
    Keywords: fusarium head blight ; Fusarium culmorum ; F. graminearum ; wheat ; mycotoxin ; deoxynivalenol ; nivalenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Aaraantasting van tarwe doorFusarium culmorum enFusarium graminearum leidt tot vorming van mycotoxinen in het graan, waarvan deoxynivalenol (DON) en nivalenol (NIV) de belangrijkste toxinen zijn. In dit artikel wordt een overzicht gegeven van de toxicologische aspecten, en het voorkomen van deze toxinen in tarwe. Informatie over DON en NIV in tarwe in West-Europa is schaars. Gebaseerd op gegevens vanFusarium epidemieën in de jaren 1979–1986 wordt een schatting gegeven van de concentratie DON in Nederlandse tarwe. Rekening houdend met de herkomst en verwerking van tarwe, blijken zowel in dierlijk als menselijk voedsel lage concentraties DON chronisch voor te komen. Op basis van een maximaal toelaatbare dagelijkse dosis DON van 3 μg kg−1 lichaamsgewicht is de schatting van de dagelijkse opname van DON in het jaar volgend op de oogst van 1982 net op de grens. Zowel een jaarlijkse inventarisatie vanFusarium aantasting en DON besmetting van het graan, als de ontwikkeling vanFusarium-resistente rassen zijn noodzakelijk.
    Notes: Summary An infection of bread wheat by fusarium head blight contaminates the crop with mycotoxins, particularly deoxynivalenol (DON) and nivalenol (NIV). The toxicity and natural occurrence of these mycotoxins in wheat are reviewed. Based on 8 years data of fusarium head blight epidemics of wheat in the Netherlands, DON contamination of the grain was estimated. Fusarium head blight ratings averaged an infection of 1.7% of all spikelets; estimates for DON contamination averaged 0.9 mg kg−1. Taking a guideline level for DON in uncleaned bread wheat of 2 mg kg−1, in 1979 and 1982 a wheat crop was produced with estimated DON concentrations above the limit of tolerance. Human and animal exposure to mycotoxins in the Netherlands appears to be small but chronic. The information presented in this paper illustrates the need for an annual evaluation of the crop for fusarium head blight incidence and mycotoxin content, and the necessity of fusarium head blight resistant wheat cultivars.
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  • 62
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; genotypes ; interaction ; manganese ; oxidation ; take-all ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Take-all is a world-wide root-rotting disease of cereals. The causal organism of take-all of wheat is the soil-borne fungus Gaeumannomyces graminis var tritici (Ggt). No resistance to take-all, worthy of inclusion in a plant breeding programme, has been discovered in wheat but the severity of take-all is increased in host plants whose tissues are deficient for manganese (Mn). Take-all of wheat will be decreased by all techniques which lift Mn concentrations in shoots and roots of Mn-deficient hosts to adequate levels. Wheat seedlings were grown in a Mn-deficient calcareous sand in small pots and inoculated with four field isolates of Ggt. Infection by three virulent isolates was increased under conditions which were Mn deficient for the wheat host but infection by a weakly virulent isolate, already low, was further decreased. Only the three virulent isolates caused visible oxidation of Mn in vitro. The sensitivity of Ggt isolates to manganous ions in vitro did not explain the extent of infection they caused on wheat hosts. In a similar experiment four Australian wheat genotypes were grown in the same Mn-deficient calcareous sand and inoculated with one virulent isolate of Ggt. Two genotypes were inefficient at taking up manganese and were very susceptible to take-all, one was very efficient at taking up manganese and was resistant to take-all, and the fourth genotype was intermediate for both characters. All genotypes were equally resistant under Mn-adequate conditions.
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  • 63
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    Euphytica 45 (1990), S. 71-80 
    ISSN: 1573-5060
    Keywords: wheat ; Triticum aestivum ; wheat leaf rust ; Puccinia recondita f.sp. tritici ; partial resistance ; histology ; growth curve
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The average size of wheat leaf rust colonies, measured using epifluorescence microscopy was significantly larger in the highly susceptible genotype Morocco than in the susceptible genotype Kaspar and the partially resistant genotypes Westphal 12A, Akabozu and BH 1146. This was already so three days after inoculation. Colony growth in partially resistant genotypes was continuously retarded compared to colonies in the highly susceptible genotype Morocco. No evidence was found for an initial inhibition of the growth of colonies in partially resistant genotypes. In partially resistant genotypes formation of uredial beds and sporulating areas started at a smaller colony size than in susceptible genotypes. Wheat leaf rust colonies in primary leaves of all genotypes studied were much larger than colonies in flag leaves measured at the same number of days after inoculation. Growth and sporulation of not intertwined colonies was not influenced by either a high or a low number of neighbouring colonies.
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  • 64
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    Euphytica 45 (1990), S. 81-86 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; wheat leaf rust ; partial resistance ; histology ; abortion ; adult plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Arrest of the growth of wheat leaf rust infection structures was studied with fluorescence microscopy in seedling leaves and flag leaves of the susceptible spring wheat genotypes Morocco and Kaspar and the partially resistant genotypes Westphal 12A and Akabozu. The percentages non-penetrants and substomatal vesicle abortion were low in all genotypes. In the partially resistant genotypes the percentage abortion of infection structures was higher than in the susceptible genotype Morocco. Aborted infection structures had formed one or two haustorial mother cells. In adult plants differences in the percentage aborted infection structures between susceptible and partially resistant genotypes were more pronounced than in seedlings. The so-called late abortion was not observed.
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  • 65
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    Euphytica 45 (1990), S. 59-69 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Pyrenophora tritici-repentis ; resistance ; tan spot ; yellow spot ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary No complete resistance to Pyrenophora tritici-repentis has been located in more than 1400 bread wheats examined. Incomplete resistance, however, occurs at different levels in many spring and winter types and data are presented for the strongest sources of resistance detected. In particular, there is a high frequency of Brazilian spring wheats with appreciable levels of resistance to this pathogen. Recent international nurseries from CIMMYT, Mexico, also contain numerous potentially valuable sources of resistance and these wheats may be shorter and higher yielding in Australia than the Brazilian wheats. The resistances in many Brazilian cultivars may be largely common because the cultivars are often strongly interrelated. Some of the Brazilian wheats resistant to P. tritici-repentis are also resistant to one or more of the septoria diseases and/or possess tolerance to aluminium toxicity.
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  • 66
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    Euphytica 45 (1990), S. 87-92 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; near-isogenic lines ; NILs ; Puccinia recondita f.sp. tritici ; leaf rust ; Puccinia striiformis ; yellow rust ; backcross ; variation ; background resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using the cultivar Arina as the recurrent parent, six backcrosses were made with two donor lines carrying the leaf rust resistance genes Lr1 and Lr9, respectively. Selection for leaf rust resistance occurred at the seedling stage in the greenhouse; the first plants transferred to the field were BC6F4s. Frequency distribution of the 332 Lr1/7 × Arina and the 335 Lr9/7 × Arina lines showed continuous variation for yellow rust resistance and heading date in these leaf rust near-isogenic lines (NILs). Similar results were also obtained for plant height, for resistance to powdery mildew and glume blotch, as well as for baking quality characters in another set of more advanced NILs. The available information on the behaviour of one of the parents of cultivar Arina led to the conclusion that the expressed yellow rust resistance is quantitative and might possibly be durable.
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  • 67
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    Euphytica 45 (1990), S. 169-177 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; coefficient of parentage ; pedigree ; gene pool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Pedigrees of 142 Yugoslavian winter wheat cultivars were traced to 110 ancestral genotypes, of which 41 contributed significantly. In each of the four major Yugoslavian wheat breeding programs, the most important ancestor, as evaluated by mean coefficient of parentage, was ‘Akagomughi’, source of the genes Rht8 and Ppd1. The other 13–19 ancestors accounting for the majority of the remaining germplasm, varied considerably among institutions. The relative contributions of ancestors changed little between the periods 1967–76 and 1982–86, with the exception of ‘Neuzucht’ (source of a 1B/1R translocation), which became much more important in the latter period.
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  • 68
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    Euphytica 46 (1990), S. 149-155 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; selection ; preharvest sprouting ; germination ; kernel color ; dormancy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The utility of spike- and seed-based mass selection techniques for improving preharvest sprouting resistance in heterogeneous wheat (Triticum spp.) populations was evaluated. Sorting seed by size improved selection efficiency in some cases, putatively by physiological synchronization. Progeny testing, as well as changes in frequency of red-kernelled types, indicate effectiveness of both spike- and seed-based mass selection for reduced preharvest sprouting. Differential effectiveness of mass selection, in populations segregating for dormancy from different sources, is consistent with previous work on mechanisms of dormancy from these sources. These results are of value to improvement of preharvest sprouting resistance in large, heterogeneous wheat populations.
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  • 69
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    Euphytica 47 (1990), S. 165-169 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; isozyme loci ; esterase ; homoeology relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The EST-6 leaf esterase phenotypes from euploid, nullisomic-tetrasomic and rye chromosome addition and substitution lines of common wheat were determined using polyacrylamide gel electrophoresis. Evidence is presented to demonstrate that Est-6 is a new set of genes, that are expressed in the leaf. The Est-6 gene set were clearly distinguished from the Est-5 genes which are expressed in the grain. The three homoeoallelic loci, Est-A6, Est-B6 and Est-D6, were located on chromosomes 3A, 3B and 3D. An Est-R6 gene was located on chromosome 6R is involved in rye. Some considerations concerning homoeology between homoeologous group 3 of wheat and the rye chromosome 6R are made.
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  • 70
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Puccinia striiformis ; yellow rust ; stripe rust ; Puccinia recondita f. sp. tritici ; brown rust ; leaf rust ; inheritance ; rust resistance ; monosomic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A set of 21 monosomics of Novosadska Rana-1 was used to locate the rust resistance genes of Lüqiyu, a stripe rust resistant line developed by BAU and Yantar, a leaf rust resistant wheat introduced from Bulgaria. The resistance of the former to p. striiformis race C25 was conditioned by a dominant gene located on chromosome 2B, whereas that of the latter to P. recondita race CL3 was controlled by two complementary dominant genes located on chromosomes 5A and 1D, respectively. The relationship of the stripe rust resistance gene in Lüqiyu to Yr5, Yr7 or Yr Suwon' all located on chromosome 2B is unknown. The two complementary leaf rust resistance factors in Yantar appear to be new.
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  • 71
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    Euphytica 48 (1990), S. 1-8 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; protein accumulation ; plant protein ; protein estimation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Better understanding of the physiological and genetic basis of wheat grain protein will contribute to breeding efforts for this characteristic. This study provides information about plant protein distribution in high and low grain protein winter wheats (Triticum aestivum L.) at different growth stages and its relation to grain protein. Field experiments involved two winter wheats with high grain protein, ‘Redwin’ and ‘Lancota’, and two with low grain protein, ‘Centurk’ and ‘Brule’ in two years. Protein content in the head, the upper three leaves, the first and second leaf, and the peduncle were estimated with Near Infrared Reflectance Spectrophotometer (NIR) at five growth stages. High protein cultivars had higher leaf protein at ripe and higher protein content in the heads at most growth stages than low grain protein cultivars. High protein cultivars had lower protein content in the peduncle than low protein cultivars at ripe. Correlation coefficients between plant-part protein and grain protein ranged from 0.48 to 0.87 for the heads, from −0.45 to −0.79 for the peduncle, and from 0.55 to 0.84 for the leaves. A combination of head, peduncle, and first leaf protein at heading was significantly related to grain protein (R2=0.71). Indirect selection for head, peduncle, and first leaf (flag leaf) protein at heading should result in increased grain protein. Recurrent selection for increased grain protein, with parent selectionbefore anthesis and hybridization should be successful.
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  • 72
    ISSN: 1573-5060
    Keywords: Sorghum bicolor ; sorghum ; Triticum aestivum ; wheat ; Oryza sativa ; rice ; Fraction-1-Protein inheritance ; Isoelectric focusing ; intergeneric hybrids ; Large and small sub-units ; rice × sorghum ; rice × wheat hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The polypeptide composition of Fraction-1-Protein (F1P) from rice × sorghum, rice × wheat hybrids and their respective parents have been analyzed by a microelectrofocusing method. The large sub-unit (LSU) is composed of three polypeptides and the small sub-unit (SSU) of two polypeptides in rice and sorghum parents and rice × sorghum hybrids. Similarly, LSU is composed of three polypeptides in the rice and wheat parents and rice × wheat hybrids. Two polypeptides occur in the SSU of rice parent and rice × wheat hybrids where as only one polypeptide in the wheat parent. These polypeptides also differ in their isoelectric points. Based on the previous reports of F1P inheritance in hybrids in other crops, F1P analysis of rice × sorghum and rice × wheat hybrids does not seem to be an important marker to identify such intergeneric hybrids. Since this is first such report of F1P inheritance in hybrids between distantly related plants, its implication in different modes of inheritance are discussed.
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  • 73
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; detached ear culture ; immature seed vernalization ; narrow-sense earliness ; photoperiodic response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of the addition of sulfurous acid into culture solution and of cold treatment of the solution were examined to simplify the culture of detached wheat ears. In the simplified method, detached ears could be cultured at room temperature on the liquid medium containing 100 g/l sucrose and 0.075% sulfurous acid without any sterilization. The immature seeds in detached ears cultured by this method were treated with low temperature or with chemicals known to have vernalizing effect. The chemical treatment did not affect the chilling requirement of immature embryos, although photoperiodic response and narrow-sense earliness were reduced by kinetin and trypsin. The low temperature treatment drastically affected the chilling requirement, and fully vernalized mature seeds having normal germinability were obtained by treating the detached ears in culture with low temperature from 10 days after anthesis.
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  • 74
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    Euphytica 49 (1990), S. 203-207 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; Sorghum bicolor ; Triticum aestivum ; C3–C4 characters ; growth analysis ; rice × sorghum ; rice × wheat hybrids ; root studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Some photosynthetic characters as-leaf anatomy, leaf photosynthetic rate and CO2 compensation pointdistinguishing C3 and C4 plants and physiological characters as leaf area ratio, nitrogen content, leaf stem ratio and total shoot-to-root and deep root-to-shoot ratios have been studied in rice × sorghum and rice × wheat hybrids. Rice × sorghum 1. has lower values of photosynthetic rate, leaf nitrogen, total root and deep root-to-shoot ratio and CO2 compensation point as of rice parent where as, rice × sorghym 2. is superior in all these characters. Both hybrids lack kranz anatomy. Though both rice × sorghum hybrids show characters of C3 rice plant but rice × sorghum 2. has improved drought tolerance and leaf characters in relation to yield. Rice × wheat hybrid have higher assimilatory area and higher total root-to-shoot ratio. Grains of rice × wheat hybrids are identical to rice grain. However, as grains of rice × wheat hybrid does not contain seed coat, it could be exploited as novel rice germplasm after improvement.
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  • 75
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    Euphytica 49 (1990), S. 155-159 
    ISSN: 1573-5060
    Keywords: Secale cereale ; rye ; rye-wheat-additions ; Triticum aestivum ; wheat ; anthocyanins ; purple leaf base ; purple/red auricles ; gene location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary ‘Purple leaf base’ is expressed only if there is anthocyanin pigmentation in coleoptiles either in rye or in rye-wheat-additions. Genes controlling ‘purple leaf base’ were found to be located on chromosomes 5R (An5), 4B (Ra2) and 6B (Ra3) using the trisomic set of rye cv. Esto and autoplasmic rye-wheat-additions, respectively.
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  • 76
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; drought resistance ; grain yield ; relative water content ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Water is often the most limiting factor to winter wheat (Triticum aestivum L.) production in the southern Great Plains of the U.S.A., yet the lack of reliable screening criteria has precluded direct selection for drought resistance in breeding programs. Previous work showed that leaf relative water content (RWC) was highly heritable when measured under field-drought conditions, but its adoption as a screening tool for yield improvement requires further investigation of the genetic relationship between grain yield and RWC. Plants representing high and low yield potential under drought stress, and a random group of plants, were selected from an F2 population having the pedigree, TAM W-101/Sturdy. Two sets of entries, each comprised of the two parents and 24 F2-derived lines, were evaluated under a rainshelter in the F3 (1986) and F4 (1987) generations to determine differences in leaf RWC during reproductive development. One set of entries did not receive any water after the jointing stage, and the other set was grown under well-watered conditions. A positive relationship was observed between grain yield and RWC measured during anthesis and mid-grain fill, as the high-yield selections maintained a significantly higher RWC than the low-yield selections. Grain yield and RWC were also positively associated among random selections segregating for both traits. Subsequent adjustment of genotype means for differences in reproductive development at time of sampling underscored the need to consider differences in maturity when RWC is the selection criterion.
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  • 77
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    Euphytica 50 (1990), S. 11-18 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Fusarium culmorum ; head blight ; scab ; resistance ; gene action ; number of genes ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crosses were made among ten winter wheat genotypes representing different levels of resistance to Fusarium head blight to obtain F1 and F2 generations. Parents, F1 and F2 were inoculated with one strain of Fusarium culmorum. Data on incidence of head blight 21 days after first inoculation were analyzed. Broad-sense heritabilities averaged 0.39 and ranged from 0.05 to 0.89 in the individual F2 families. The joint-scaling test indicated that the inheritance of Fusarium head blight resistance was adequately described by the additive-dominance model, with additive gene action being the most important factor of resistance. With respect to the non-additive effects, dominance of resistance predominated over recessiveness. The number of segregating genes governing resistance in the studied populations was estimated to vary between one and six. It was demonstrated that resistance genes differed between parents and affected resistance differently.
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  • 78
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat breeding ; genetic improvement with time ; grain nitrogen concentration ; nitrogen economy ; nitrogen utilization efficiency ; Argentina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Changes in nitrogen (N) economy and N to dry matter (DM) relationships were studied for six cultivars of bread wheat (Triticum aestivum L.) released in Argentina at different times between 1912 and 1980. Experiments were performed on two successive years. N partitioning to reproductive organs was changed both at anthesis and at maturity. Grain N yield (GNY) was associated to both total N accumulated and N partitioning. Most of the changes produced by genetic improvement on N economy at maturity could be explained by parallel changes at anthesis. Neither biological N yield (BNY) at anthesis nor BNY at maturity showed any trend with the year of release of the cultivars. Grain N concentration (GNC) showed a negative trend with the year of release, and was inversely correlated to both grain yield (GY) and harvest index (HI). However, GNC was positively and significantly associated with NHI to HI ratio, indicating that the main reason for its behaviour along this century was the dilution of N on non N compounds. The N utilization efficiencies (NUE) for both GY and grain number were positively affected by breeding. Moreover, modern Argentinian cultivars are as efficient as the best cultivars showed by other authors. It is suggested that to increase GNC together with GY, breeders should improve N accumulation at anthesis maintaining high remobilization of vegetative N.
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  • 79
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    Euphytica 50 (1990), S. 1-9 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Fusarium culmorum ; Fusarium head blight ; resistance ; scab ; diallel cross ; combining ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten homozygous winter wheat genotypes representing different levels of resistance to Fusarium head blight were crossed in all possible combinations excluding reciprocals. Parents, F1 and F2 were inoculated with one pathogenic strain of Fusarium culmorum. Data for head blight, observed 21 days after first inoculation (OBS-2), and for the area under the disease progress curve, based on observations 14, 21 and 28 days after first inoculation (AUDPC), were analyzed. The contrast between parents and F1 crosses indicated dommance effects of the resistance genes. Diallel analysis according to Griffing's Method 4, Model 1 showed significant general combining ability (GCA) effects for both F1 and F2; specific combining ability effects were not significant. With the exception of one genotype for which general performance for Fusarium resistance was not in agreement with its GCA, the resistance to F. culmorum was uniformly transmitted to all offspring, and the parents can be described in terms of GCA. It is suggested that in the progenies with one of the awned lines as parent, one resistance gene was linked with the gene coding for presence of awns, located on chromosome 4B. A single observation date, taken at the right time, was as effective in assessing resistance as the AUDPC.
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  • 80
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    Euphytica 50 (1990), S. 221-239 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; gibberellic acid response ; dwarfing genes ; culm length
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The GA response, Rht genes and culm length of 133 Norin varieties, 6 breeding lines and 16 landraces of Japanese wheat were investigated. Out of 133 Norin varieties tested, 103 were GA-insensitive and 30 GA-responsive. The 6 breeding lines were all GA-insensitive. Out of 16 landraces tested, 10 were GA-insensitive and 6 GA-responsive. Among the 10 GA-insensitive landraces, only Daruma had a Rht1 genotype. The other 9 had a Rht2 genotype. None of the landraces tested carried both Rht1 and Rht2 or Rht3. Out of the 103 GA-insensitive Norin varieties, 22 carried only Rht1, another 79 carried only Rht2, and only Norin 10 and Kokeshikomugi carried both Rht1 and Rht2. No tested variety carried Rht3. Some Norin varieties carrying Rht2 showed tall culms comparable to that of the rht tester line Chinese Spring. These results suggest that these varieties had a nullifier or modifier gene(s) or height promoting genes in the background controlling the height-reducing effect of Rht2. Conversely, six GA-responsive Norin varieties were as short as Akakomugi which carries the GA-responsive Rht genes, Rht8 and Rht9. The also seemed to carry a GA-responsive Rht gene or genes, and moreover all but one may carry gene(s) other than the Akakomugi genes. The origin of Rht1 and Rht2 of Norin 10 was speculated on the GA-response and Rht genotypes of its related varieties and landraces.
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  • 81
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    Euphytica 50 (1990), S. 57-62 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; single plant selection for yield ; inter-genotypic competition ; interplot competition ; cultivar degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study was undertaken to explore the relation between auto-, allo- and nil-competition using a long established pure line cultivar of bread wheat, where genes controlling yielding and competing ability co-exist and co-interact. The investigation lasted three growing periods, the first two with trials grown at nilcompetition (100 cm), and the third with trials grown at the three conditions of competition. In the first growing period, divergent single plant honeycomb selection for high and low yield was applied in the cultivar Siete Cerros, to continue in the second growing period with divergent line honeycomb selection. In the third growing period, top and bottom lines selected under nil-competition were compared with the mother variety Siete Cerros under auto-, allo- and nil-competition. Correlation coefficient estimates between auto-, allo- and nil-competition demonstrated that auto-competition and nil-competition are correlated positively interse and negatively with allo-competition. In general, the results suggest that yielding and competing ability are correlated negatively. The implications of the findings are discussed in relation to the possibility of (1) selecting for heritable high yield on a single plant basis, (2) avoiding biased results due to interplot competition, (3) substituting cultivar degeneration for cultivar improvement.
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  • 82
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    Euphytica 51 (1990), S. 77-86 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; dough stickiness ; rye-derived wheat cultivars
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rye-derived wheat cultivars are being used in many breeding programmes throughout the world in order to achieve improvements in yield and disease resistance. However, the serious quality defect of intense dough stickiness associated with many of these wheat cultivars is limiting the usefulness of their flour in large mechanised bread bakeries. A dough preparation procedure was developed which enabled the dough surface properties of a range of rye-derived wheat cultivars to be assessed on doughs mixed quantitatively to their optimum mixing time. Intense dough stickiness was found in samples of 1AL/1RS and 1DL/1RS translocation lines tested and in all of the 1BL/1RS wheat cultivars examined except the West German cultivar, Disponent. Most of the 1BL/1RS wheat cultivars were derived from the Russian cultivars, Kavkaz, Aurora and Skorospelka 35 and included the CIMMYT-bred cultivars such as the Veery lines (Glennson, Ures, Genaro and Seri) and the Nebraskan cultivar, Siouxland. Based on the results of studying selected 1BL/1RS wheat cultivars in detail, this intense dough stickiness appeared to be independent of growing season, trial location, protein content, mixing tolerance, milling process and extraction rate. In addition pilot bakery trials confirmed that our laboratory testing procedures can be used to detect this intense dough stickiness.
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  • 83
    ISSN: 1573-5036
    Keywords: biological control ; Gaeumannomyces graminis var.tritici ; take-all ; Trichoderma harzianum ; T. koningii ; T. hamatum ; pyrones ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Antagonism tests on agar-plates and glasshouse screening indicated that three isolates ofTrichoderma harzianum varied in their ability to antagonize the take-all fungus (Gaeumannomyces graminis var.tritici). Isolate 71 which was the most effective in suppressing take-all of wheat, produced two pyrones and other undetermined analogues. Isolates ofT. koningii andT. hamatum shown to suppress take-all, produced a simple pyrone compound. AlthoughT. harzianum isolates 70 and 73 did not produce any pyrones, they reduced the disease albeit to a much lesser extent than isolate 71; with isolate 73 showing distinct host growth promotion effects. It is proposed that the success of isolate 71 ofT. harzianum was related to the pyrones it produces and that the ability of isolates 70 and 73 to reduce take-all may be related to mechanisms other than those involving antibiotics.
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  • 84
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    Plant and soil 123 (1990), S. 223-227 
    ISSN: 1573-5036
    Keywords: aluminium ; accumulation ; aneuploid ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Preliminary studies indicated that aluminium-tolerance in wheat (Triticum aestivum L. Thell.) is a dominant character controlled by several genes. The present paper describes further work on localization and characterization of some of these genes in the genome of the medium Al tolerant wheat cultivar Chinese Spring (C.S.), using an aneuploid series (ditelosomics). Aluminium-tolerance of seedlings was assessed using the modified ‘pulse’ method; the aluminium concentration in the nutrient solution causing irreversible damage to the root apical meristems on exposure for 24 h at 25°C was the measure of Al-tolerance. At least three different factors controlling Al-tolerance in the C.S. cultivar were located on chromosomes 5As, 2Dl and 4Dl. Significant differences were found in Al-uptake and accumulation in roots of the respective ditelosomic lines and euploid seedlings of C.S. Genes controlling Al-tolerance located in the D genome (2Dl and 4Dl) were not expressed in solution culture when genes located on 5As were missing, whereas some tolerance was observed in aneuploid lines in which genes from 5As were present while genes from 2Dl and 4Dl were missing. It is concluded that Al-tolerance genes located in A genome control the expression of other Al-tolerance genes located in the D genome. The implications of the obtained results for chromosome and gene manipulations in cereals are discussed.
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  • 85
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    Plant and soil 124 (1990), S. 141-142 
    ISSN: 1573-5036
    Keywords: denitrification ; Gaeumannomyces graminis var. tritici ; potassium deficiency ; rhizosphere ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat inoculated with the root pathogen Gaeumannomyces graminis var. tritici (Ggt) was grown in quartz silt at two levels of potassium nutrition. While in plants well supplied with K the incidence of Ggt did not affect plant growth, it reduced shoot and root weight of K deficient plants. Denitrification, measured by the acetylene inhibition technique and expressed as N2O/mg root weight, was increased either by low K nutrition or by Ggt infection. Highest denitrification in the rhizosphere of plants was found with a combination of both, K deficiency and Ggt attack.
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  • 86
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    Plant and soil 128 (1990), S. 143-151 
    ISSN: 1573-5036
    Keywords: barley ; calcium ; humidity ; magnesium ; membrane leakage ; salinity ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Salinity-calcium interactions, which have been shown to be important in plants grown in dryland saline soils of the Canadian prairies, were studied in two species differing in salt tolerance. In solution culture, wheat showed a greater reduction in growth and a higher incidence of foliar Ca deficiency symptoms than barley when grown under MgSO4 or Na2SO4 plus MgSO4 salt stress. Amendment of the saline solution with Ca to increase the Ca/(Na+Mg) ratio ameliorated the effects of salt, but more so in wheat than in barley. At least part of the difference in salt tolerance between the two species must therefore relate to species differences in the interaction of salinity and Ca nutrition. The greater response of wheat to Ca was not due to a lower Ca status in leaf tissue; on the contrary, although Ca amendments improved tissue Ca/(Na+Mg) ratios in both species, salinized wheat had equivalent or higher Ca content, and higher Ca/(Na+Mg) ratios than did barley. The higher Ca requirement of wheat is apparently specific to a saline situation; at low salinity, wheat growth was not reduced as extensively as that of barley as Ca/(Na+Mg) ratio was decreased. High night-time humidity dramatically improved wheat growth under saline conditions, but increasing the Ca concentration of the saline solution had no effect on growth in the high humidity treatment. Membrane leakage from leaf tissue of wheat grown under saline conditions was increased compared to tissue from non-saline plants. Plants grown in Ca-amended saline solutions showed no increase in membrane leakage. These results confirm the importance of Ca interaction with salinity stress, and indicate differences in species response.
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  • 87
    ISSN: 1573-5036
    Keywords: calcium ; copper ; desert ; ground water ; iron ; magnesium ; manganese ; nitrogen ; phosphorus ; Prosopis glandulosa ; rooting patterns ; sodium ; symbiotic nitrogen fixation ; trace metals ; water table ; water use efficiency ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Mesquite plants (Prosopis glandulosa var. Torreyana) were grown in 2-m long columns 20 cm in diameter, and provided with a constant, stable ground water source 10 cm above the sealed base of the column. Ground water contained 0, 1 or 5 mM nitrate, or a mixed salt solution (1.4, 2.8, or 5.6 dS m-1) with the ionic ratios of ground water found in a field stand of Prosopis at Harper's Well (2.8 dS m-1). Water uptake in the highly salinized columns began to decrease relative to low salt columns when soil salinity probes 30 cm above the column base read approximately 28 dS m-1, a potential threshold for mesquite salt tolerance. Prosopis growth increased with increasing nitrate, and decreased with increasing salinity. Water use efficiency was little affected by treatment, averaging approximately 2 g dry matter L-1 water used. Most fine roots were recovered from a zone about 25 cm above the ground water surface where water content and aeration appeared to be optimal for root growth. Root-shoot ratio was little affected by nitrate, but increased slightly with increasing salinity. Plant tissue P concentrations tended to increase with increasing salinity and decrease with increasing N, approaching potentially deficient foliage concentrations at 5 mM nitrate. The whole-plant leaf samples increased in sodium concentration both with added salt and with added nitrate. Foliar manganese concentrations increased with increasing salt in the absence of nitrate. Concentrations of sodium in leaves were low (〈10 g kg-1), considering the high salt concentrations in the ground water. Prosopis appears to exclude sodium very effectively, especially from its younger leaves. Although Prosopis is highly salt tolerant, the degree to which it utilizes soil nitrate in place of biologically fixed N may lower its salinity tolerance and affect its nutrient relations in phreatic environments.
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    Plant and soil 125 (1990), S. 239-244 
    ISSN: 1573-5036
    Keywords: ammonium ; calcium ; CO2 exchange rate ; GS/GOGAT ; rice rubisco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract It is commonly known that calcium promotes NO3 - uptake in many crop species. However, calcium enhancement of NH4 + uptake by plants has received little attention. This study aimed to evaluate the effect of Ca supplements on NH4 + uptake and plant growth in solution cultured rice. Supplemental Ca applied at vegetative and reproductive phases of plant ontogeny tended to stimulate NH4 + absorption, and accordingly resulted in a better straw and grain yield. However, excessively supplied Ca (400 ppm) was detrimental to plant growth. Increases in straw and grain yield observed at Ca levels up to 300 ppm were linked to the Ca-enhanced activities of glutamine synthetase (GS), glutamate synthase (GOGAT), and ribulose 1, 5-bisphosphate carboxylase/oxygenase (Rubisco).
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  • 89
    ISSN: 1573-5036
    Keywords: burr medic ; grass-infestation ; productivity ; re-establishment ; subterranean-clover ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field-plot experiment investigated the re-establishment and productivity in 1987 (following wheat (Triticum aestivum) in 1986) of burr medic (Medicago polymorpha) and subterranean (sub-) clover (Trifolium subterraneum), which were each sown with 0, 20, 40, 60, 80, or 100% grass in 1985. There was no difference in the amount of dry matter production by medic or sub-clover over the whole growing season but medic was more productive earlier and sub-clover more productive later. Grass generally had little effect on legume or total dry matter production at proportions 〈40%, though medic productivity was slightly more vulnerable to the effect of grass-infestation than sub-clover.
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  • 90
    ISSN: 1573-5036
    Keywords: barley ; chlorosis resistance ; cucumber ; genotypical differences ; grasses ; iron mobilization ; iron uptake ; maize ; microorganisms ; oat ; phytosiderophores ; rice ; root exudates ; root growth ; rye ; sorghum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Graminaceous species can enhance iron (Fe) acquisition from sparingly soluble inorganic Fe(III) compounds by release of phytosiderophores (PS) which mobilize Fe(III) by chelation. In most graminaceous species Fe deficiency increases the rate of PS release from roots by a factor of 10–20, but in some species, for example sorghum, this increase is much less. The chemical nature of PS can differ between species and even cultivars. The various PS are similarly effective as the microbial siderophore Desferal (ferrioxamine B methane sulfonate) in mobilizing Fe(III) from a calcareous soil. Under the same conditions the synthetic chelator DTPA (diaethylenetriamine pentaacetic acid) is ineffective. The rate of Fe(III)PS uptake by roots of graminaceous species increases by a factor of about 5 under Fe deficiency. In contrast, uptake of Fe from both synthetic and microbial Fe(III) chelates is much lower and not affected by the Fe nutritional status of the plants. This indicates that in graminaceous species under Fe deficiency a specific uptake system for FePS is activated. In contrast, the specific uptake system for FePS is absent in dicots. In a given graminaceous species the uptake rates of the various FePS are similar, but vary between species by a factor of upto 3. In sorghum, despite the low rate of PS release, the rate of FePS uptake is particularly high. The results indicate that release of PS and subsequent uptake of FePS are under different genetic control. The high susceptibility of sorghum to Fe deficiency (‘lime-chlorosis’) is most probably caused by low rates of PS release in the early seedling stage. Therefore in sorghum, and presumably other graminaceous species also, an increase in resistance to ‘lime chlorosis’ could be best achieved by breeding for cultivars with high rates of PS release. In corresponding screening procedures attention should be paid to the effects of iron nutritional status and daytime on PS release as well as on rapid microbial degradation of PS.
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  • 91
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    Plant and soil 124 (1990), S. 33-37 
    ISSN: 1573-5036
    Keywords: leaf area ; nitrogen ; mineral nutrition ; phosphorus ; photoperiod ; Triticum ; wheat ; spikelet initiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of N and P on the number of spikelets of spring wheat (Triticum aestivum L.), grown in nutrient solution, were studied under 8 h and 16 h photoperiods. The effect of P was apparent only at a high rate of N supply and the effects of N were increased significantly at a high rate of P supply. Increasing N supply increased the number of spikelets due to a promotion of the rate of spikelet initiation. It also increased the leaf-blade area and the dry matter weight of the plants at the stage of terminal spikelet initiation. These effects of N were much greater under the short photoperiod than under the long photoperiod. The practical significance of these findings for winter-grown wheat in temperate regions is pointed out.
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  • 92
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    Plant and soil 128 (1990), S. 97-101 
    ISSN: 1573-5036
    Keywords: calcium ; magnesium ; nitrogen ; nitrogen saturation ; Norway spruce ; nutritional imbalance ; Picea abies ; soil solution ; sulphur/nitrogen ratio ; tree nutrition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Differences in nitrogen cycling and in the nutrition of trees are significantly coupled to the levels of nitrogen input and to the nitrate levels in the soil solution. Relatively high nitrogen supply can cause unbalanced nutrition on sites which contain either low or moderate amounts of other nutrients. This is indicated by low cation/nitrogen ratios in foliage and also by the S/N ratio falling temporarily below 0.030.
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  • 93
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    Euphytica 45 (1990), S. 113-118 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; spring varieties ; Vrn-genotypes ; dominant Vrn-genes ; geographic distribution ; landraces and improved varieties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A study of the global Vrn-genes distribution supports the hypothesis that these loci have different breeding values. Evident zonal differences were discovered in percentage of dominant Vrn-genes and genotypes between the sets of varieties from various locations. For the same zone analogous differences were not so manifested between the sets of local and improved varieties. It showed the similar environmental fitness of those sets as a result of natural or artificial selection. Obvious changes in distribution of Vrn-genes were discovered in the history of breeding. Within this century the dominant Vrn3 was introgressed into many modern varieties of countries close to the equator. The main direction of such introgression ran from the Japanese landrace Akakomugi to Italian varieties. Later it came from Italy through Mentana to Mexican semidwarf varieties, and the last ones (especially Sonora 64 and Lerma Rojo 64) promoted wide distribution of that gene all over the world.
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  • 94
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; indirect selection ; single-plant selection ; honey-comb design ; harvest index ; protein content ; correlation ; regression ; path coefficients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The relationships between yield, its components and other associated characteristics, both within and across generations, were studied in the F2, F3 and F4 of two Hard Red Spring wheat (Triticum aestivum) crosses using simple correlation, path coefficient and step-wise multiple regression analyses. In the F2 and F3 the plants were grown 50 cm apart from each other while in the F4 they were grown under the usual farm practices. Selection was practiced for high and low yield in the F2 and F3 mainly on the basis of individual plant yield. Statistically significant, but not always practically useful, correlations were found between yield and its components and other associated characters. The relationship between yield and protein content was negative and significant within all generations but not so between F2 (and F3) and F4. The intergeneration correlation coefficients between F4 grain yields and grain yields measured in the F2 and F3 were all positive and highly significant. These coefficients, which are also heritability estimates in standard units, were small in magnitude. Stepwise multiple regression analysis identified plant yield as the most significant factor in determining F4 line yield, followed by its components in the order of 1000-kernel weight, grain yield per plant and number of tillers per plant. Path coefficient analysis identified tiller number per plant and grain yield per spike as having strong positive direct effects on single plant yield. Harvest index of individual F2 plants can be used as an indirect selection criterion for yield.
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  • 95
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Hordeum chilense ; Triticum turgidum ; Triticum durum ; durum ; C-banding ; meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The meiotic behaviour of a hybrid between Triticum aestivum and the amphiploid Hordeum chilense x T. turgidum conv. durum, was studied using a C-banding staining method. This hybrid has the genome formula of AA BB D Hch with 2n=6x=42 chromosomes. The durum wheat chromosomes (genomes A and B) were easily recognized, whereas the D and Hch chromosomes were recognized as a whole. Meiotic pairing was homologous, as expected (14 bivalents from A and B genomes +14 univalents from D and Hch genomes). However, some pollen mother cells at metaphase-I presented pseudobivalents that could have been caused by either homoeologous or autosyndetic pairing amongst D and Hch chromosomes.
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  • 96
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    Euphytica 46 (1990), S. 51-56 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; yield potential ; dwarfing genes ; Norin-10 ; Tom Thumb ; yield components ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A composite convergent cross of 16 spring wheat parents produced a set of unselected progeny lines among which the major dwarfing genes, Rht1, Rht2 and Rht3, were distributed against a common random genetic background. Random subsets of these lines were grown under irrigation and optimal conditions in 4 experiments with replicated bordered plots in southern New South Wales in order to measure the dwarfing gene effect on yield potential. The dwarfing gene composition of each line was determined by test crossing and seedling responsiveness to gibberellic acid. Lodging was negligible in the two experiments in 1982. While present in the two in 1983, it was not strongly associated with yield. Grain yield levels were appropriately high (mean 5.9 t/ha). In all but 1 experiment the Rht1+Rht2 dwarf genotypes gave highest yields while the Rht3 group yielded on average 3% lower, Rht2 9% lower, Rht1 11% lower, and the non-dwarf or tall group yielded 24% lower. These yield differences were positively associated with harvest index, kernels per m2 and kernels per spike, but negatively associated with mature plant height. Even within major dwarfing gene classes, grain yield was significantly and negatively associated with height.
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  • 97
    ISSN: 1573-5060
    Keywords: Triticum spp. ; wheat ; x triticosecale ; triticale ; Mycosphaerella graminicola ; Septoria tritici ; septoria tritici blotch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The relationships between percent pycnidia coverage on the four uppermost leaves (PCD), plant height (PHT) and days to heading (HED) were evaluated for 21,000 wheat and triticale accessions tested in artificially inoculated (with fixed combination of S. tritici isolates) field nurseries over 8 trial years. A general Linear Model procedure (GLM) estimated Septoria severity using two correlative models: model 133-1 Year and model II−PCD=b1PHT+b2HED+C. The regression coefficients for PHT and HED in the two models were −0.54 and −0.40, respectively, with a R2=0.80** and R2=0.29** for model I and model II, respectively. The predicted cultivar best fitted to the model would be characterized as a semidwarf (PHT=115 cm) with an early-moderate maturity (HED=95 days to heading). The estimated mean percent pycnidial coverage for the two models over the 8 trial years was 40.8%. The performance of a group of 38 cultivars replicated yearly during the 8 trial years was assessed relative to model I. The deviation of each cultivar from the model was calculated using two functions: a) Sum Relative Serial Deviation (SRSD) and b) Total Relative deviation (TRD), in addition to Standard errors (SE). The proposed analytical protocol enabled identification of cultivars which expressed consistent yearly deviation (from the model) in host response combined with low-moderate mean pycnidial coverage (±30%). Such cultivars may possess a more stable type of genetic protection against the adverse effects of septoria tritici blotch.
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  • 98
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    Euphytica 47 (1990), S. 49-55 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Sr31 ; 1BL/1RS translocation ; sticky dough problem ; dough un-mixing time ; mixing tolerance ; over-mixing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The dough handling properties of a number of Sr31 and non-Sr31 wheats were examined in the laboratory test bake procedure using a National test bake mixer. Dough stickiness was not apparent in any of the wheats at optimum dough development. The baking quality at optimum dough development of Sr31 wheats was comparable to non-Sr31 wheats. However, after optimum dough development Sr31 wheats broke down and exhibited dough stickiness more rapidly with continued mixing than non-Sr31 wheats. A new dough parameter is introduced, dough un-mixing time, and is defined as the time from the point of optimum dough development to the point where the dough breaks down as a result of continued mixing to produce a sticky, non-cohesive mass. It is shown that Sr31 wheats have shorter dough un-mixing times than non-Sr31 wheats, and that dough un-mixing times for both Sr31 and non-Sr31 wheats are influenced by environmental factors, particularly those which determine grain protein content. A selection strategy for breeding Sr31 wheats with commercially acceptable dough properties is indicated by placing Sr31 in a genetic back-ground of high quality gluten proteins, and selecting for long dough un-mixing times.
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  • 99
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    Euphytica 47 (1990), S. 121-130 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; heritability ; protein inheritance ; genotype x environment interaction ; variance components ; indirect selection ; grain protein content ; grain yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Increasing grain protein content is an important wheat breeding goal. Noaman & Taylor (1988b) showed the combination of protein content in the head, peduncle, and flag leaf of winter wheat at heading provided a good estimate for grain protein. The objectives of this research were to apply these results in indirect selection scheme for grain protein improvement and to study the heritability of protein content in these plant parts. Two random winter wheat populations from four parents in double crosses were used in this study. Sixty randon F2-derived F5 and F6 lines were grown in randomized complete block design with 3 replicates in two years. Significant differences for grain yield, grain protein, and vegetative protein content were detected among F5 and F6 lines in both populations. Genotypic and phenotypic correlations between grain protein and vegetative protein were significant and in agreement. Estimates of narrow sense heritability of protein content using variance components method ranged from 0.46 to 0.94 for leaf 2 and head in population 1, and from 0.63 to 0.89 for peduncle and head in population 2. Correlation coefficients (r) between predicted and observed grain protein ranged from 0.50 to 0.88 and from 0.37 to 0.84 in populations 1 and 2, respectively. The highest r was obtained from the combination of head, peduncle, and flag leaf protein at heading. Correlation between protein in plant parts and grain yield was small and not significant. The high heritability of vegetative protein at heading allows the identification of genotypes before pollination which are likely to produce high grain protein. Indirect selection for head, peduncle, and flag leaf protein should result in increased grain protein without yield reduction noted in other breeding schemes.
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  • 100
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    Euphytica 47 (1990), S. 203-214 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Septoria tritici ; septoria tritici blotch ; resistance ; Mycosphaerella graminicola
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary All possible crosses (including reciprocals) were made among four winter bread (Aurora, Bezostaya 1, Kavkaz, and Trakia) and two Israeli spring wheat cultivars (spring x winter diallel), and among two South American spring wheats (Colotana and Klein Titan) with the same Israeli cultivars (spring x spring diallel) to study the inheritance of resistance to septoria tritici blotch. Parents, F1, F2 and backcrosses were grown in two separated blocks in the field over two years. One block was inoculated with isolate ISR398A1 and another with ISR8036. Each plant was assessed for plant height (cm), days to heading (from emergence or transplanting), and percent pycnidia coverage on the four uppermost leaves. Plant height and maturity had insignificant effects on pycnidia coverage. No cytoplasmic effects could be detected. In the spring x winter diallel general combining ability (GCA) was the major component of variation. Significant specific combining ability (SCA) was present in all cases. Partial dominance was operative in populations inoculated with ISR398A1. Resistance in the winter wheats was controlled by a small number of genes (usually two). The four winter wheats derive their resistance to ISR398A1 from their common parent Bezostaya 1 which lacks the 1B/1R wheat-rye translocation. Their resistance is readily overcome by ISR8036. Inheritance of the South American wheats can be explained by additive effects, with a small number of genes of recessive mode affecting resistance to both isolates. Breeding strategies that favor additive, and additive x dominance gene action should be pursued.
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