Publication Date:
1987-03-27
Description:
A large collection of good genetic markers is needed to map the genes that cause human genetic diseases. Although nearly 400 polymorphic DNA markers for human chromosomes have been described, the majority have only two alleles and are thus uninformative for analysis of genetic linkage in many families. A few known marker systems, however, detect loci that respond to restriction enzyme cleavage by producing a fragment that can have many different lengths. This polymorphism is due to variation in the number of tandem repeats of a short DNA sequence. Because most individuals will be heterozygous at such loci, these markers will provide linkage information in almost all families. Ten oligomeric sequences derived from the tandem repeat regions of the myoglobin gene, the zeta-globin pseudogene, the insulin gene, and the X-gene region of hepatitis B virus, were used to develop a series of single-copy probes. These probes revealed new, highly polymorphic genetic loci whose allele sizes reflected variation in the number of tandem repeats.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nakamura, Y -- Leppert, M -- O'Connell, P -- Wolff, R -- Holm, T -- Culver, M -- Martin, C -- Fujimoto, E -- Hoff, M -- Kumlin, E -- New York, N.Y. -- Science. 1987 Mar 27;235(4796):1616-22.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3029872" target="_blank"〉PubMed〈/a〉
Keywords:
*Chromosome Mapping
;
Chromosomes, Human/analysis
;
Cosmids
;
DNA Restriction Enzymes/metabolism
;
Genes, Viral
;
Globins/genetics
;
Hepatitis B virus/genetics
;
Humans
;
Pedigree
;
Polymorphism, Genetic
;
*Repetitive Sequences, Nucleic Acid
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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