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  • Lepidoptera  (28)
  • Nitrogen fixation  (27)
  • Coleoptera
  • Springer  (75)
  • 1980-1984  (75)
  • 1950-1954
  • 1982  (75)
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  • Springer  (75)
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  • 1980-1984  (75)
  • 1950-1954
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  • 1
    ISSN: 1432-2048
    Keywords: Alnus ; Ammonium ; Carbon translocation ; Endophyte damage ; Nitrogen fixation ; Root nodule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cloned plants of Alnus incana (L.) Moench were inoculated and grown without combined nitrogen for seven weeks. The effects of ammonium on the function and structure of the root nodules were studied by adding 20 mM NH4Cl (20 mM KCl=control) for four days. Nitrogenase activity decreased to ca. 50% after one day and to less than 10% after two days in ammonium treated plants, but was unaffected in control plants. The results were similar at photon flux densities of 200 and 50 μmol m-2 s-1. At the higher light level the effect was concentration dependent between 2 and 20 mM NH4Cl. The recovery was slow, and more than 11 d were needed for plants treated with 20 mM ammonium to reach initial activity. The distribution of 14C to the root nodules after assimilation of 14CO2 by the plants was not changed by the ammonium treatment. Microscopical studies of root nodules showed high frequencies of endophyte vesicles being visually damaged in nodules from ammonium-treated plants, but not in nodules from control plants. When nitrogenase activity was restored, visually damaged vesicles were again few, whereas young developing vesicles were numerous. The slow recovery, the 14C-translocation pattern, and the structural changes of the endophyte indicate a more complex mechanism of ammonium influence than simply a short-term reduction in supply of carbon compounds to the nodules.
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  • 2
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    Archives of microbiology 132 (1982), S. 219-224 
    ISSN: 1432-072X
    Keywords: Rhizobium ; Nitrogen fixation ; Nodules ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several mutants defective in nodulation were isolated from Rhizobium japonicum strains 3I1b110 and 61A 76. Mutants of class I do not form nodules after incubation with soybean [Glycine max (L.) Merrill] for 17 days, but will do so by 28 days. When host plants other than G. max are infected with several of these strains, there is no detectable difference in the time of nodulation or size of nodules as compared to the wild type. Two mutants of class I (i. e., SM1 and SM2) have been shown previously to be altered in the lipopolysaccharide portion of their cell wall. Mutants of class II are not slow to nodulate but form fewer nodules than the wild type on all the host plants tested. Mutants of class III are unable to form nodules. Some bacteriophage-resistant mutants, altered in cell surface structure, fall into this class. Two mutants of class III do not bind to soybean roots.
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  • 3
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    Archives of microbiology 132 (1982), S. 333-337 
    ISSN: 1432-072X
    Keywords: Anabaena variabilis Kütz ; 14C-prelabeled blue-green algae ; Interaction respiration/photosynthesis ; CO2 exchange ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Prelabeled Anabaena variabilis Kütz. evolves 14CO2 in the light with KCN and DCMU (2,4-dichlorophenyl-1,1-dimethylurea) present, comparable to the dark control without inhibitors added. Double-reciprocal plots of CO2 release vs. light intensity with either KCN or KCN+DCMU present result in two straight lines intersecting at the ordinate. Apparently, reducing equivalents originating from carbohydrate catabolism are channeled into the photosynthetic electron-transport chain, competing for electrons from photosystem II. Under these conditions, the CO2 release is accompanied by a light-dependent oxygen uptake, presumably due to oxygen-reducing photosystem-I activity while ribulose-bisphosphate carboxylase is inhibited by KCN. Comparing nine blue-green algae it was shown that only nitrogen-fixing species release substantial amounts of CO2 in the light with KCN or KCN+DCMU present. This release is particularly obvious with Anabaena variabilis Kütz. under nitrogen-fixing conditions, but small when the alga is grown with combined nitrogen. We conclude that nitrogen-fixing species share a common link between respiratory and photosynthetic electron transport. The physiological role may be electron supply of nitrogenase by photosystem I.
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  • 4
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    Archives of microbiology 133 (1982), S. 6-10 
    ISSN: 1432-072X
    Keywords: Rhodospirillaceae ; Rhodopseudomonas globiformis ; Nitrogen metabolism ; Nitrogen fixation ; Glutamine synthetase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhodopseudomonas globiformis strain 7950 grew with a variety of amino acids, urea, or N2 as sole nitrogen sources. Cultures grown on N2 reduced acetylene to ethylene; this activity was absent from cells grown on nonlimiting NH 4 + . Glutamate dehydrogenase could not be detected in extracts of cells of strain 7950, although low levels of an alanine dehydrogenase were present. Growth ofR. globiformis on NH 4 + was severely inhibited by the glutamate analogue and glutamine synthetase inhibitor, methionine sulfoximine. High levels of glutamine synthetase (as measured in the γ-glutamyl transferase assay) were observed in cell extracts of strain 7950 regardless of the nitrogen source, although N2 and amino acid grown cells contained somewhat higher glutamine synthetase contents than cells grown on excess NH 4 + . Levels of glutamate synthase inR. globiformis were consistent with that reported from other phototrophic bacteria. Both glutamate synthase and alanine dehydrogenase were linked to NADH as coenzyme. We conclude thatR. globiformis is capable of fixing N2, and assimilates NH 4 + primarily via the glutamine synthetase/glutamate synthase pathway.
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  • 5
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    Archives of microbiology 133 (1982), S. 312-317 
    ISSN: 1432-072X
    Keywords: Rhizobium japonicum ; Rhizobium leguminosarum ; Formate metabolism ; Formate dehydrogenase ; Nitrogen fixation ; Nitrogenase ; Bacteroids ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Formate metabolism supported nitrogen-fixation activity in free-living cultures of Rhizobium japonicum. However, formate0dependent nitrogense activity was observed only in the presence of carbon sources such as glutamate, ribose or aspartate which by themselves were unable to support nitrogenase activity. Formate-dependent nitrogenase activity was not detected in the presence of carbon sources such as malate, gluconate or glycerol which by themselves supported nitrogenase activity. A mutant strain of R. japonicum was isolated that was unable to utilise formate and was shown to lack formate dehydrogenase activity. This mutant strain exhibited no formate-dependent nitrogenase activity. Both the wild-type and mutant strains nodulated soybean plants effectively and there were no significant differences in the plant dry weight or total nitrogen content of the respective plants. Furthermore pea bacteroids lacked formate dehydrogenase activity and exogenously added formate had no stimulatory effect on the endogenous oxygen uptake rate. The role of formate metabolism in symbiotic nitrogen fixation is discussed.
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  • 6
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    Archives of microbiology 133 (1982), S. 172-177 
    ISSN: 1432-072X
    Keywords: Beggiatoa ; Nitrogen fixation ; Acetylene reduction ; Nitrate assimilation ; Microaerobic ; Isolation of marine strains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four newly isolated marine strains of Beggiatoa and five freshwater strains were tested for nitrogen fixation in slush agar medium. All strains reduced acetylene when grown microaerobically in media containing a reduced sulfur source and lacking added combined nitrogen. The addition of 2 mmol N, as nitrate or ammonium salts, completely inhibited this reduction. Although not optimized for temperature or cell density, acetylene reduction rates ranged from 3.2 to 12 nmol·mg prot-1 min-1. Two freshwater strains did not grow well or reduce acetylene in medium lacking combined nitrogen if sulfide was replaced by thiosulfate. Two other strains grew well in liquid media lacking both combined nitrogen and reduced sulfur compounds but only under lowered concentrations of air. All freshwater strains grew well in medium containing nitrate as the combined nitrogen source. Since they did not reduce acetylene under these conditions, we infer that they can assimilate nitrate.
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  • 7
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    Theoretical chemistry accounts 60 (1982), S. 579-587 
    ISSN: 1432-2234
    Keywords: Nitrogen fixation ; Nitrogen complexes ; Carbon monoxide complexes ; Electronic effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract MNDO method is used to study the interaction of nitrogen and carbon monoxide molecules with a proton, hydrogen atom, hydride ion, hydrogen molecule ion and hydrogen molecule. Predicted geometries and heats of reaction of different complexes are presented. The wave functions are analyzed in terms of ground state charge distributions and overlap populations. Electronic effects accompanying complexation are also discussed.
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  • 8
    ISSN: 1432-2048
    Keywords: Ammonium assimilation ; Glycine ; Nitrogen fixation ; Proplastid ; Purine synthesis ; Root nodule ; Ureide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Subcellular organelle fractionation of nitrogen-fixing nodules of soybean (Glycine max (L.) Merr.) indicates that a number of enzymes involved in the assimilation of ammonia into amino acids and purines are located in the proplastids. These include asparagine synthetase (EC 6.3.1.1), phosphoribosyl amidotransferase (EC 2.4.2.14), phosphoglycerate dehydrogenase (EC 1.1.1.95), serine hydroxymethylase (EC 2.1.2.1), and methylene-tetrahydrofolate dehydrogenase (EC 1.5.1.5). Of the two isoenzymes of asparate aminotransferase (EC 2.6.1.1) in the nodule, only one was located in the proplastid fraction. Both glutamate synthase (EC 1.4.1.14) and triosephosphate isomerase (EC 5.3.1.1) were associated at least in part with the proplastids. Glutamine synthetase (EC 6.3.1.2) and xanthine dehydrogenase (EC 1.2.1.37) were found in significant quantities only in the soluble fraction. Phosphoribosylpyrophosphate synthetase (EC 2.7.6.1) was found mostly in the soluble fraction, although small amounts of it were detected in other organelle fractions. These results together with recent organelle fractionation and electron microscopic studies form the basis for a model of the subcellular distribution of ammonium assimilation, amide synthesis and uredie biogenesis in the nodule.
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  • 9
    ISSN: 1573-1561
    Keywords: Defense ; glands ; Coleoptera ; Chrysomelidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Defense glands were examined in the adults of 65 species belonging to 10 different subfamilies. They were found in the pronota and elytra of members of the subfamilies Criocerinae, Chrysomelinae, Galerucinae, and Alticinae. It is suggested that these glands appeared monophyletically in the course of evolution and that the absence of glands in several species of the two most evolved subfamilies is a secondary event, explained by the presence of alternative efficient defensive behaviors: reflex bleeding in the Galerucinae and escape mechanism of jumping in the flea beetles. It is also suggested that a large distribution of the glands at the surface of the beetles is a primitive condition and that in the course of evolution only the glands most efficiently located along the edges of the pronotum and elytra were maintained. Such evolution has occurred several times. Alternative and complementary defensive mechanisms are also listed and discussed.
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  • 10
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    Journal of chemical ecology 8 (1982), S. 31-39 
    ISSN: 1573-1561
    Keywords: Malacosoma americanum ; eastern tent caterpillar ; Lepidoptera ; Lasiocampidae ; trail pheromone ; pheromone secretory site ; silk trail
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A new site of secretion of a chemical trail marker was found on the sternum at the tip of the last abdominal segment of the larva of the eastern tent caterpillarMalacosoma americanum. Larvae marked from this site by drawing their sterna along the substrate when they extended existing trails in search of food and again when they established recruitment trails to food-finds. Differences in the quantity or quality of the marker deposited by exploring and recruiting caterpillars may account for the greater activity of the recruitment trails.
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  • 11
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    Journal of chemical ecology 8 (1982), S. 125-136 
    ISSN: 1573-1561
    Keywords: Aggregation ; pheromone ; elm ; mark-release ; multilure ; Coleoptera ; Scolytidae ; Scolytus multistriatus ; trap-out ; Ulmus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Two experiments were conducted to investigate the use of a pheromone-based trap-out technique for suppressing populations ofScolytus multistriatus. In the first experiment, elm bolts containing the immature stages ofS. multistriatus were placed in an isolated community that contained elm trees, but which was essentially devoid of a resident beetle population. The infested bolts produced a total of 46,485 adult beetles of which 20% were recovered on traps baited with synthetic pheromone. In the second experiment beetles were released in a desert valley containing no resident elms or beetles. Only 1% of 20,000 released beetles were recaptured on traps erected on vertical cardboard cylinders and on elm logs. These rates of recapture are related to the attraction of beetles to naturally occurring brood sources versus pheromone-baited traps.
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  • 12
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    Journal of chemical ecology 8 (1982), S. 267-273 
    ISSN: 1573-1561
    Keywords: Fall armyworm ; Spodoptera frugiperda ; Lepidoptera ; Noctuidae ; (Z)-9-dodecen-1-ol acetate ; (Z)-9-tetradecen-1-ol acetate ; pheromone ; mating behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Two components of the fall armyworm,Spodoptera frugiperda (J.E. Smith), sex pheromone, (Z)-9-dodecen-1-ol acetate (DDA) and (Z)-9-tetradecen-1-ol (TDA), were tested alone and in combination to determine their effects on male sexual response and inhibition of mating in the laboratory. The threshold response for FAW males was lower for TDA than for DDA, and males responded to TDA over a wider range of dosages. Although TDA is not attractive to FAW males in the field, this compound was highly effective in reducing mating under laboratory conditions.
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  • 13
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Noctuidae ; Pseudaletia separata ; male scent ; repellent pheromone ; repellent allomone ; nonspecificity ; sexual selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Air movement around a malePseudaletia separata Walker, observed using the smoke of an incense stick during wing-fanning, showed that the air moves away from the female and backwards. Moths moved or flew away immediately when vaporized benzaldehyde was blown over their antennae in a screen cage set in a greenhouse. This repellency lacked species, sex, or individual specificity. Male scent acts as an inhibitor to conspecific males and, at the same time, to other moths if they approach the male in courtship. Inhibition of ovipositional and larval locomotory behavior by benzaldehyde was also demonstrated.
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  • 14
    ISSN: 1573-1561
    Keywords: Agrotis segetum ; turnip moth ; Lepidoptera ; Noctuidae ; sex pheromone ; straight-chain acetates ; single-cell recordings ; electroantennography ; behavior ; gas chromatography-mass spectrometry ; entrainment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Analysis of female abdominal tips ofAgrotis segetum by means of GC-MS showed the presence of 13 aliphatic acetates and alcohols. (Z)-7-Dodecenyl acetate was found to be the main component in the extracts at amounts of about 1 ng/female. (Z)-9-Tetradecenyl acetate and (Z)-7-dodecenol were present to the extent of 49 and 19%, respectively, of the main component. Minor components could be identified as decyl acetate, (Z)-5-decenyl acetate, dodecyl acetate, (Z)-9-dodecenyl acetate, tetradecyl acetate, a tetradecenyl acetate, hexadecyl acetate, a hexadecenyl acetate, (Z)-5-decenol, and (Z)-9-tetradecenol. The presence and biological activity of decyl acetate, (Z)-5-decenyl acetate, and (Z)-7-dodecenyl acetate in the extracts could be detected by GC-EAD. Tested by EAG (Z)-5-decenyl acetate evoked the highest response among pheromone candidates, followed by (E)-5-decenyl acetate and (Z)-7-dodecenyl acetate. Single-cell recordings from 100 male antennal sensilla trichodea revealed receptorcells highly sensitive to (Z)-5-decenyl, (Z)-7-dodecenyl, (Z)-8-dodecenyl, and (Z)-9-tetradecenyl acetate as well as (Z)-5-decenol. The (Z)-5-decenyl, (Z)-7-dodecenyl, and (Z)-9-tetradecenyl acetate receptors were activated significantly also by female extracts. When tested in a tube olfactometer, a blend of decyl, (Z)-5-decenyl, (Z)-7-dodecenyl, and (Z)-9-tetradecenyl acetate evoked the same male response as did female glands. Tested in the field, this blend was more attractive than virgin females. Other authors previously reported many of the compounds identified in the present study. However, both quantitative and qualitative discrepancies exist among the various investigations, possibly due to the existence of geographical races.
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  • 15
    ISSN: 1573-1561
    Keywords: Pheromone ; multistriatin ; 4-methyl-3-heptanol ; brevicomin ; frontalin ; Scolytus multistriatus ; Scolytus scolytus ; Scolytus pygmaeus ; Coleoptera ; Scolytidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Laboratory bioassays and field tests demonstrated that a Swiss population ofS. multistriatus responded much more strongly to α- than to δ-miltistriatin in combination with 4-methyl-3-heptanol and α-cubebene. High concentrations of brevicomin appeared to replace α-multistriatin in evoking a response byScolytus species, but this effect can be explained by the fact that the brevicomin was contiminated with small amounts of α-multistriatin. Frontalin, another bicyclic ketal, showed no biological activity. Field tests indicated thatS. pygmaeus aggregates to the same attractant mixture asS. multistriatus. S. scolytus also responded preferentially to this mixture, but the relative amounts of α-multistriatin to 4-methyl-3-heptanol do not appear to be as important as forS. multistriatus.
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  • 16
    ISSN: 1573-1561
    Keywords: Smaller tea tortrix moth ; Adoxophyes sp. ; Lepidoptera ; Tortricidae ; asymmetric induction ; 10-methyl-1-dodecanol acetate ; ester ; chirality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A new route to (±)-10-methyl-1-dodecanol acetate, a minor component of the smaller tea tortrix moth (Adoxophyes sp.), is described. An optional sequence that permits the generation of the chiral center with enantiomeric excesses (ee's) as high as 74% (R) or 80% (S) employing the available (S)-(−)-prolinol as a chiral auxiliary may be included. High-performance liquid chromatography of diastereomeric intermediates allows preparation of products with greater ee's.
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  • 17
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    Journal of chemical ecology 8 (1982), S. 233-239 
    ISSN: 1573-1561
    Keywords: Choristoneura fumiferana ; Lepidoptera ; Tortricidae ; carbohydrates ; sucrose ; feeding preference ; behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Spruce budworm larvae reared on an artificial diet were tested for preference of twelve carbohydrates over a water control. A strong preference for sucrose was seen, followed by fructose, inositol, and glucose. Male and female larvae do not differ in their responses to sucrose. The behavioral threshold for sucrose is at 10−4 to 10−3 M, with a peak response in the range of 0.01 to 0.05 M sucrose.
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  • 18
    ISSN: 1573-1561
    Keywords: Western avocado leafroller ; Amorbia cuneana ; sex pheromone ; Tortricidae ; (E,E)- and (E,Z)-10,12-tetradecadien-1-ol acetate ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The major volatile components in the extract of the female sex pheromone gland ofAmorbia cuneana consisted of (E,E)- and (E,Z)-10,12-tetradecadien-1-ol acetates. The identification was based on electroantennogram bioassay of gas Chromatographic effluent from sex pheromone gland extract, relative retention times on polar and nonpolar gas chromatographic columns, chemical degradation (ozonolysis, saponification), mass spectrometry, chemical synthetic methods, and field tests. Based on mass spectrometry and retention times by capillary gas chromatography, traces of (E)-10-tetradecen-1-ol acetate and 1-tetradecanol acetate were also present in the extract. Traps baited with a combination of synthetic (E,E)- and (E,Z)-10,12-tetradecadien-1-ol acetates caught more males than did traps baited with females.
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  • 19
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    Journal of chemical ecology 8 (1982), S. 1251-1262 
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Tortricidae ; Adoxophyes orana ; pheromone traps ; attraction ; interaction ; trap spacing ; wind ; monitoring ; mass trapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In a spindle-tree apple orchard, sex pheromone traps for the summerfruit tortrix moth were distributed at different spacings. From the captures, information was derived about influence of prevailing wind direction and of trap spacing on interactions between traps. Prevailing wind direction in the experimental area had no appreciable influence on trap interaction, perhaps because of eddying within the planting. The relationship between density and capture of traps was first considered theoretically and interaction by overlap of active-space areas was distinguished from that by overlap of mere capturing areas, which depend also on distance of dispersal. The actual results were in accordance with these considerations and indicated that the diameter of the active-space areas averaged 15 m. The variation in the captures of the wider-spaced traps was too large to be conclusive about the average width of the capturing areas, but the diameter of these seemed to be more than about 45 m. These data have been related to adequate trap distances for monitoring and mass trapping.
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  • 20
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; Scolytus multistriatus ; pheromone ; insect olfaction ; electroantennogram ; electrophysiology ; differential adaptation ; multistriatin ; 4-methyl-3-heptanol ; α-cubebene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electroantennograms were recorded fromScolytus multistriatus in response to 4-methyl-3-heptanol, the four geometric isomers of multistriatin, and cubeb oil. Charateristic dose-response curves for response amplitude and the time required for the voltage trace to return to 1/2 baseline (recovery rate) were established. Recovery rates were significantly more rapid following stimulation with 4-methyl-3-heptanol or cubeb oil than with the multistriatin isomers. At most intensities, α-multistriatin, the isomer that evokes behavioral response, gave significantly larger EAGs with significantly longer recovery rates than the other isomers. Results of differential adaptation experiments suggested that 4-methyl-3-heptanol interacted with the processes involving multistriatin and cubeb oil activity. However, cross-activity of acceptors for these compounds seems unlikely; single sensillum recordings are needed to ascertain the response spectra for individual receptor neurons.
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  • 21
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    Journal of chemical ecology 8 (1982), S. 1399-1409 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; Dendroctonus ponderosae ; pheromone ; frontalin ; exo-brevicomin ; Pinns contorta ; Pinus ponderosa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Frontalin andexo-brevicomin were identified by GC-MS in air drawn over maleDendroclonus ponderosae Hopk. (MPB) from Oregon that had joined females for 1–2 days in the bark of lodgepole and ponderosa pine logs. Unfed males releasedexo- andendo-brevicomin but not frontalin. These three compounds were not detected in either unfed or fed females. Arrestment of males bytrans-verbenol and terpenes in an olfactory walkway was reduced by the addition of racemic frontalin; production of attractant chirps also diminished. Racemic frontalin also strongly reduced the aggregation of MPB in lodgepole and ponderosa pine stands to sticky traps baited with the aggregation pheromonetrans-verbenol and host terpenes; however, the function of the natural enantiomer of frontalin in MPB is unknown.
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  • 22
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    Journal of chemical ecology 8 (1982), S. 1411-1420 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Staphylinidae ; Oxytelinae ; defensive secretion ; glands ; acetates ; lactones ; alkenes ; quinones ; citral
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The secretions of the abdominal glands ofBledius spectabilis Kraatz,Platystethus arenarius Fourcr., andOxytelus piceus L. have been shown to contain four 1-alkenes; toluquinone; toluhydroquinone; C10 −-,C11 −, C12 −-, and C14 − γ-lactones; C12-δ-lactone; citral; and decyl-, undecyl-, and dodecyl acetates. Quantitative results indicate that 1-alkenes are formed probably from present lactones by decarboxylation. According to the known life histories of the beetles, it is suggested that the gland material is not used as an algal growth regulator but represents a unique defensive blend characteristic for the whole subfamily.
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  • 23
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    Journal of chemical ecology 8 (1982), S. 1429-1436 
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Noctuidae ; Heliothis zea ; bioassay ; host plant resistance mechanisms ; allelochemicals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Maysin, a flavone glycoside from corn silks, inhibits ingestion, and thus growth, ofHeliothis zea (Boddie) larvae. Pinitol from soybeans inhibitedH. zea growth by the same mechanism. Despite the widely held assumption that tannins inhibit growth by inhibiting assimilation, cotton condensed tannin inhibitedH. zea growth by reducing ingestion; no evidence was found for a reduction in assimilation. Neonate larvae are shown to be much more sensitive to allelochemics than larvae that have fed on control diet before being transferred to diet containing plant allelochemics.
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  • 24
    ISSN: 1573-1561
    Keywords: Red flour beetle ; Tribolium castaneum ; Coleoptera ; Tenebrionidae ; food volatiles ; olfactory responses ; olfactometer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Olfactory responses of 72- to 96-hr-old, 24-hr starved adult male, female, or mixed-sex groups of the red flour beetle,Tribolium castaneum (Herbst), to volatiles from wheat and millet kernels, certain milled fractions, and solvent extracts were recorded by using a lightsensitive apparatus. Wheat-germ volatiles from intact germ or solvent extracts were generally more attractive than volatiles of wheat endosperm or wheat bran. Volatiles of whole wheat kernels were the least attractive among test materials of wheat origin, whereas there were no significant differences among whole wheat flour, germ, and endosperm. Wheat-germ extracts, however, were more attractive than were extracts of the other fractions. Whole millet flour or fermented millet flour volatiles were more attractive than those from whole millet kernels or millet starch. Beetles reached maximum responsiveness to grain volatiles by 72–96 hr after adult ecdysis. Groups of virgin female beetles generally were more responsive than male or mixed-sex groups to volatiles of substances tested.
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    Plant and soil 64 (1982), S. 251-253 
    ISSN: 1573-5036
    Keywords: Competition of Rhizobium ; Nitrogen fixation ; Nodulation ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Competition of five strains of Rhizobium of the cowpea group, onVigna radiata (L) Wilcjeck variety ML 5, was tested in loamy clay and loamy sand soils. Strains RM 6 and RM 5 were effective nodulators in loamy clay soil, and strains MNH, M 20 and RM 6 were effective nodulators in loamy sand soil. Strains RM 6 and MNH predominated nodule formation in loamy clay and loamy sand soils respectively.
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  • 26
    ISSN: 1573-5036
    Keywords: Isotope techniques ; Nitrogen-15 ; Nitrogen fixation ; Nitrogen utilization ; Phaseolus vulgaris ; Rhizobium ; Varietal comparison
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Differences in N2-fixation byPhaseolus vulgaris bean cultivars were successfully evaluated in the field using15N isotope dilution technique with a non-fixing test crop of a different species (wheat). The Phaseolus cultivars could have been similarly ranked for N2-fixation capacity from either seed yield or total nitrogen yield, but the isotope method provided a direct measure of N2-fixation and made it possible to estimate the proportion of fixed to total nitrogen in the crop and in plant parts. Amounts of nitrogen fixed varied between 24.59 kg N/ha for the 60-day cultivar Goiano precoce to 64.91 kg N/ha for the 90-day cultivar Carioca. The per cent of plant nitrogen due to fixation was 57–68% for the 90-day cultivars and 37% for Goiano precoce (60-day cultivar). Fertilizer utilization was 17–30% of a 20 kg N/ha fertilizer application. 100 kg N/ha fertilizer application decreased N2-fixation without suppressing it totally. Differences in yield between the highest yielding (Carioca) and the lowest (Moruna) 90-day cultivars were also due apparently to varietal differences in efficiency of conversion of nitrogen to economic matteri.e. seed, as well as to differences in capacity of genotypes for N2-fixation.
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    Plant and soil 65 (1982), S. 383-396 
    ISSN: 1573-5036
    Keywords: Frankia ; Microbial ecology ; Nitrogen fixation ; Purshia ; Revegetation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nitrogen fixing trees and shrubs may be useful in revegetation efforts. Speculation that insufficient endophyte populations in surface soils may limit non-leguminous symbiotic nitrogen fixation in marginal land was explored.Purshia tridentata andP. glandulosa seedlings were grown in greenhouse trials using ten soils from nativePurshia sites. Treatments include a control, an inoculated treatment, and six mmole nitrogen amendment. When inoculated with aP.tridentata crushed nodule inoculum, two of five non-nodulating soils and three sparsely nodulating soils produced well nodulated plants. Inoculation also increased nodule mass, total nitrogen, nitrogen content and shoot dry mass in plants from some of the soils. Of the three soils failing to produce nodulated plants when inoculated, one produced plants that responded well to nitrogen additions but failed to nodulate under low nitrogen conditions; another produced severely stunted plants indicating nutritional limitations on the host; and the third produced plants that were not nitrogen deficient. An application of nitrogen completely suppressed nodulation in all but one soil. The twoPurshia species were similar in nodulation, nitrogen fixation and growth, although important exceptions exist that indicate species may differ in adaptability to certain soil conditions.
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  • 28
    ISSN: 1573-5036
    Keywords: Acridine orange ; Cochliobolus sativus ; Common root rot ; Nitrogen fixation ; Nuclear staining ; Roots ; Senescence ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Natural senescence of the root cortex was assessed by nuclear staining, for cultivars and chromosome substitution lines of spring wheat known to differ in (1) susceptibility to common root rot, (2) total rhizosphere populations and (3) ability to support growth of a free-living nitrogen-fixing bacterium. Together, three root rot susceptible wheat lines showed significantly more cortical senescence than did three resistant lines; the susceptible lines also support larger rhizosphere populations. The wheat line that supports growth of a nitrogen-fixing bacterium showed significantly less cortical death than did any other line. Substitution of chromosome pairs 5B or 5D between the parent cultivars Rescue and Cadet substantially altered the amount of root cortex death, which is thus genetically determined. It is suggested thatCochliobolus sativus and other weak parasites benefit from early natural senescence of the root cortex, and that the degree of susceptibility or resistance of wheat lines to common root rot is at least partly determined by differences in cortical senescence.
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    Plant and soil 68 (1982), S. 55-67 
    ISSN: 1573-5036
    Keywords: Klebsiella ; Nitrogen fixation ; Oryza sativa ; Phyllosphere ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The beneficial effect of spraying some highly active phyllosphere N2-fixing microorganisms on high and low yielding cultivars of rice plants as compared with that of urea applied at different doses are described. The dry weight, N-content, 1000 grain weight, and yield were remarkably increased in all cases with the application of phyllosphere microorganisms. The performance of two isolates KUP4 and KUPBR2 with IR-8 and IR-26 rice, was better than that of 52 kg urea-N per hectare. IR-579 rice leaves in association with some phyllosphere bacteria reduced acetylene at the rate of 664–816 nmoles/g leaf/h. In IR-26 rice the effect of application of bacterial suspension at three phases of plant growth corresponded very well with that of urea application in three split doses under identical conditions. Recommended fertilizer rates produced the same yield as the half dose plus bacterial spray in the cultivars Pankaj and Rupsail. Fertilizer application in Pankaj and Rupsail rice reduced nitrogenase activity and the beneficial effects of phyllosphere N2-fixation was reduced by 40–55%.
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    Plant and soil 68 (1982), S. 217-222 
    ISSN: 1573-5036
    Keywords: Nitrogen fixation ; Oryza sativa ; Phragmites communis ; Reed ; Rhizosphere ; Rice ; Roots ; Soil temperature
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The relation of nitrogenase activity (ethylene evolution) to soil temperature or incubation temperature of roots was determined on two genera of swamp plants, namely rice (Oryza sativa) cultivated in tropical climate and reed (Phragmites communis) grown in temperate regions. For both intact rice plants and excised rice roots the optimum temperature was 35°C. On excised roots nitrogenase activity responded more sensitivity to changes in temperature. In contrast to intact rice plants no ethylene evolution occurred on excised roots at 17 and 44°C. On reed roots temperature optimum was between 26 and 30°C which is clearly lower than on rice (35°C). The temperature range in which nitrogen fixation occurred was, however, similar to that of rice, although on a lower level. The results suggest a higher potential of the tropics for associative N2 fixation, while in cooler climates the lower temperatures appear to be a major limiting factor.
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    Plant and soil 69 (1982), S. 293-297 
    ISSN: 1573-5036
    Keywords: Ecology ; Frankia ; Nitrogen fixation ; Water potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Growth responses of Frankia isolates to decreasing water potential were monitored in systems where potentials were controlled by KCl, NaCl and Polyethylene glycol. The highest potential tested was −2 bar (basal medium). The general pattern emerging was that isolates fromAlnus glutinosa, A. viridis andComptonia peregrina showed declining growth at potentials below −2 to −5 bar. AMyrica gale isolate showed declining growth with decreasing potential. All isolates were more sensitive to decreases in potential in a matric controlled than an osmotic controlled system. They all showed approximately 50 percent growth reduction at −5 to −8 bar, and meagre growth at −16 bar after 35 days. The Comptonia isolate was the most vigorous at low potentials. Nitrogen fixation ability was monitored for two isolates. Highest specific activities were observed between −3 and −5 bar for the Myrica isolate and between −5 and −7.5 bar for theA. glutinosa isolate.
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  • 32
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    Plant and soil 64 (1982), S. 263-266 
    ISSN: 1573-5036
    Keywords: Blue-green algae ; Nitrogen fertilisers ; Nitrogen fixation
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of ammonium or nitrate-nitrogen on biological nitrogen fixation by an algal crust are compared. Nitrate-nitrogen up to 3.0 μmoles N g−1 sand/algal crust at 60% water holding capacity did not affect fixation, whereas an ammonium-nitrogen concentration of 0.2 μmoles N g−1 crust markedly depressed fixation. Consequences of these differential effects are considered.
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  • 33
    ISSN: 1573-5036
    Keywords: Aluminium toxicity ; Nitrogen fixation ; Nodulation ; Stylosanthes hamata ; Stylosanthes humilis ; Stylosanthes scabra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effects of three solution aluminium concentrations (0, 25, and 100μM) on nitrogen fixation by well-nodulated plants ofStylosanthes hamata, Stylosanthes humilis andStylosanthes scabra are reported. Plants were inoculated with Rhizobium CB756 and grown for 21 days in an aluminium-free nutrient solution at pH 5.3 before imposition of the aluminium treatments. Nitrogen fixation was measured both by the increase in total nitrogen content of the plants and acetylene reduction in roots of plants harvested at 10 and 20 days after imposition of the aluminium treatments. Solution aluminium concentrations as high as 100μM, had no detrimental effect on nitrogen fixation in any species.
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  • 34
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    Plant and soil 69 (1982), S. 341-352 
    ISSN: 1573-5036
    Keywords: Barley ; Nitrogen fixation ; Rhizoplane ; Rhizosphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Aerobic and anaerobic N2-fixing bacteria developed in the rhizosphere of barley seedlings and exhibited N2ase activity when seedlings were grown in sterilized sand-nutrient cultures containing low levels of combined nitrogen. The source of the N2-fixing bacteria appeared to be the seed. Average daily rates up to 0.9 μmoles C2H4 h−1 g−1 dry root tissue were measured, but the intensity of the activity was affected by moisture levels and concentration of combined N in the rhizosphere. Removal and washing of the roots did not remove the activity, and roots remained active even after surface-sterilization. An unidentified aerobic N2-fixing bacterium was isolated from the rhizoplane of active barley roots. Inoculation of barley seedlings with the aerobic N2-fixing bacterium enhanced N2ase activity of excised roots 10-fold, with average rates of 0.9, 1.1 and 1.3 μmoles h−1 g−1 dry root assayed under pO2 of 0.01, 0.02 and 0.04 atm respectively. The aerobic N2-fixing bacterium also exhibited N2ase activity when inoculated into the rhizosphere of oat, rice and wheat seedlings. Microscopic observations of sterilized live and stained barley roots suggest that the aerobic N2-fixing bacterium is an endophyte which infects root tissue and metamorphoses into vesicle-like structures.
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  • 35
    ISSN: 1573-5036
    Keywords: Drought stress ; Nitrogen fixation ; Nodulation ; Prosopis spp ; Semi-arid ; Tree legumes ; Water potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The literature does not contain a field report of nodulation for the xerophytic tree legumes of the genus Prosopis despite their widespread occurrence in semi-arid regions of the world. A phraetophytically simulated greenhouse experiment was conducted with mesquite in a 3m deep soil column to determine if nodules would form in deper depths where moisture is more abundant. The upper 0.5m in the soil column was allowed to dry to 2200 kPa but the 3.2 m depth was maintained at soil moistures more positive than 70 kPa by water additions to the bottom of the soil column. Over 100 nodules and an acetylene reduction rate of 1.9 mg/h were observed at the 3.2m depth. Nodulation or acetylene reduction were not observed closer than 2.7m from the surface. Air temperatures during these assays exceeded 45°C. Leaf xylem water potentials were in the 2800–3500 kPa range.
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    Plant and soil 66 (1982), S. 21-28 
    ISSN: 1573-5036
    Keywords: Agro-forestry ; Acetylene reduction ; Leucaena leucocephala ; Nematode root knots ; Nitrogen fixation ; Nodule biomass ; Woody legume
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The nitrogen fixation rate in a 4-year-old stand of the woody legumeLeucaena leucocephala (Lam.) de Wit. was estimated in the field at a rather dry site in Tanzania by use of an acetylene reduction technique. The diurnal mean value during April–May was 35 nmol C2H4 mg−1 (dry weight) nodules h−1, with a variation between 22±8 and 48±12 nmol C2H4 mg−1 (dry weight) nodules h−1 in early morning and at midday, respectively. The nodule biomass was determined by auger sampling to be 51±16 kg (dry weight) ha−1. Most of the nodules were found at the 10–30 cm soil depth level. A rough calculation of the amount of nitrogen fixed annually arrived at 110±30 kg ha−1. The results give strong support for the use ofL. leucocephala for soil enrichment in less humid areas of tropical Africa.
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  • 37
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    Plant and soil 66 (1982), S. 125-127 
    ISSN: 1573-5036
    Keywords: Charcoal ; Garden pea ; Inoculation ; Nitrogen fertilisation ; Nitrogen fixation ; Nodulation ; Pisum sativum ; Wood charcoal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In a factorial pot experiment on garden peas investigating the effect of growing medium, inoculation, nitrogen fertilisation and charcoal addition, shoot growth was significantly better in John Innes potting compost than in 50/50 peat/sand compost and when receiving nitrogen fertilisation but was depressed by the presence of wood charcoal. In the peat/sand medium, root systems were smaller but carried more and larger nodules. The number of nodules was increased by inoculation but the addition of carbon decreased root size, nodule number and nodule size.
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    Plant and soil 66 (1982), S. 413-416 
    ISSN: 1573-5036
    Keywords: Growth ; Nematicide ; Nitrogen fixation ; Nodulation ; Peanut ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of two granular nematicidesviz. oxamyl and fenamiphos, on the nodulation and growth of Rhizobium inoculatedArachis hypogaea L. was studied in glasshouse and field trials. In the glasshouse trial at the suggested rates of application shoot fresh weight was significantly reduced by oxamyl whilst root fresh weight was similarly affected by fenamiphos. In the field trial vegetative growth and plant emergence were significantly reduced by both nematicides. Nodulation at the higher rates of application was increased by both oxamyl and fenamiphos whilst oxamyl caused a significant increase in pod number at the highest rate of application.
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  • 39
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    Plant and soil 68 (1982), S. 321-329 
    ISSN: 1573-5036
    Keywords: Alginate ; A value ; Endomycorrhiza ; Glomus mosseae ; Inoculant ; Nitrogen fixation ; Rhizobium japonicum ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This field study was undertaken to determine the effect of inoculation withGlomus mosseae on N2 fixation and P uptake by soybean. The inoculation withGlomus mosseae was achieved using a new type of inoculant, alginate-entrapped (AE) endomycorrhizal fungus. N2 fixation was assessed using the A value method. In P-fertilized plots, inoculation with AEGlomus mosseae increased the harvest index based on dry weight (+20%) and N content of seeds (+17%), the A value (+31%) and %N derived from fixation (+75%). Inoculation with AEGlomus mosseae decreased the coefficient of variation for the A value and for the dry weights of the different plant parts.
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  • 40
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    Plant and soil 69 (1982), S. 233-238 
    ISSN: 1573-5036
    Keywords: Nitrogen fixation ; Azospirillum spp. ; Azospirillum lipoferum ; Wheat yield
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seven isolates belonged toA. brasilense and 3 belonged toA. lipoferum. Isolates having more denitrifying capacity fixed less nitrogen in nitrogen free semi-solid malate medium. One strain ofA. lipoferum having high nitrogen fixing capacity with negative test for denitrification was tested as inoculant to supplement the nitrogen need of a wheat crop in field condition with different doses of N with and without the inoculant. While control without nitrogen yielded 1260 kg/ha the yield in inoculated treatment was 2070 kg/ha resulting in significant increase. In a treatment receiving 40 kg N/ha the grain yield was 2370 kg/ha as against yield of 3110 kg/ha in a similar treatment receiving fertiliser plus inoculant. Thus increase in yield was about 30%. Further the treatment receiving 80 kg N/ha yielded 2970 kg/ha as against yield of 4150 kg/ha in a treatment receiving inoculant alongwith the above dose of the fertiliser. Thus increase in yield due to application of inoculant was about 36%. Similarly, the uptake of N in different treatments was augmented due to inoculation of seeds with the culture.
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  • 41
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    Plant and soil 69 (1982), S. 265-273 
    ISSN: 1573-5036
    Keywords: Azotobacter chroococcum ; Fertilizers ; Farmyard manure ; Nitrogen ; Nitrogen fixation ; Potassium ; Phosphorus ; Yields of grain stover ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field trials were conducted during theKharif seasons of 1979 and 1980 to examine the effect of the nitrogen-fixing bacteriumAzotobacter chroococcum (isolate M4) on yields of maize and N-economy. Different levels of nitrogen and farmyard manure (FYM) were supplied to assess their interaction with Azotobacter inoculation. Seed inoculation without fertilization raised grain and stover yields significantly. FYM coupled with inoculation gave higher yields than either could singly. The financial gain due to an increase in grain yield upon applying per ha 80 kg N, 10×103 kg FYM and Azotobacter over the treatment 40N + FYM + Azotobacter was offset by the cost of the additional N; thus, the higher N-application was not economical. Azotobacter inoculation was economically most efficient at lower doses of fertilizer nitrogen which not only increased yields but resulted in a saving of fertilizer N when applied in combination with FYM.
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    Plant and soil 69 (1982), S. 275-280 
    ISSN: 1573-5036
    Keywords: Azotobacter chroococcum ; Fertilizers ; Farmyard manure ; Zea mays ; Maize ; Nitrogen ; Nitrogen fixation ; Phosphorus ; Potassium ; Total nitrogen uptake
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The economic feasibility of using cultures of nitrogen fixing microorganisms in programmes to increase crop production, as a selfgenerating source of nitrogen, has been proved beyond doubtviz. Legume-Rhizobium symbiosis and blue green algal ‘fertilizer’ for rice. The extent to which the free living, N-fixing, aerobic, heterotropicAzotobacter chroococcum could replace the application of nitrogenous fertilizer to maize was investigatedin vivo. Total nitrogen uptake (kg ha−1) by maize after inoculation with Azotobacter combined with moderate applications of nitrogen fertilizer and farmyard manure was influenced significantly and resulted in a higher nitrogen concentration in grain and stover along with a higher yield.
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    Plant and soil 69 (1982), S. 391-399 
    ISSN: 1573-5036
    Keywords: Associative nitrogen fixation ; Inoculation ; Nitrogen fixation ; Root exudate ; Soil bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Organic components leaked fromSorghum bicolor seedlings (‘root exudates’) were examined by recovering14C labelled compounds from root solutions of seedlings inoculated withAzospirillum brasilense, Azotobacter vinelandii orKlebsiella pneumoniae nif-. Up to 3.5% of the total14C recovered from shoots, roots, and nutrient solutions was found in the root solutions. Inoculation with Azospirillum and Azotobacter increased the amounts of14C and decreased the amounts of carbohydrates in the root solutions. When sucrose was added as a carbon source for the bacteria, the increase of14C in the solutions did not occur. Quantities of14C found in the root solutions were proportional to amounts of mineral nitrogen supplied to the plants. Bacterial growth also was proportional to nitrogen levels. When sorghum plants were grown in soil and labelled with14CO2, about 15% of the total14C recovered within 48 hours exposure was found in soil leachates.
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    Journal of chemical ecology 8 (1982), S. 351-362 
    ISSN: 1573-1561
    Keywords: Choristoneura occidentalis ; western spruce budworm ; Lepidoptera ; Tortricidae ; pheromone chemistry ; Choristoneura fumiferana ; capillary GC-MS ; (E/Z)-11-tetradecenal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract SCOT capillary Chromatographic and SCOT capillary chromatographic-mass spectrometic analyses of gland washes and effluvia of virgin femaleChoristoneura occidentalis Free, have been conducted with both a diapausing and nondiapausing strain of this insect. The following compounds were identified in gland washes and effluvia in both strains:E andZ11–14∶Ald,E andZ11–14∶Ac,E andZ11–14∶OH and 14∶Ald, 14∶Ac, and 14∶OH. The average aldehyde: acetate: alcohol ratio found by analysis of single glands by virgin females (nondiapausing strain) was 1∶7∶0.73. Analysis of virgin female effluvia gave this ratio as 10∶3∶8 (diapausing strain: %Z=8, 11, 15, respectively) and 10∶3∶6 (nondiapausing strain: %Z=8, 11, 12, respectively). The saturated components were generally 1–2% of theE isomer in each case. Comparisons of EAG responses of bothC. occidentalis andC. fumiferana toE11–14∶Ald,E11–14∶Ac andE11–14∶OH were made. Correlations with both laboratory and field data previously published were also made betweenC. fumiferana andC. occidentalis.
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    Journal of chemical ecology 8 (1982), S. 373-378 
    ISSN: 1573-1561
    Keywords: Dendroctonus frontalis ; Ips calligraphus ; I. grandicollis ; I. avulsus ; Coleoptera ; Scolytidae ; mating ; attraction
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Catches ofDendroctonus frontalis andIps avulsus on traps surrounding bolts infested with both sexes of each species in the gallery were not significantly different from catches at bolts infested only with the sex which normally pioneers the host colonization process. In contrast, the presence of the second sex in the gallery significantly reduced catches ofI. grandicollis andI. calligraphus and, additionally, the presence of females in the male galleries ofI. calligraphus significantly reduced the catch ofI. avulsus females as compared with their response to bolts infested with the maleI. calligraphus only.
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    Journal of chemical ecology 8 (1982), S. 453-462 
    ISSN: 1573-1561
    Keywords: Defensive secretions ; oleic acid ; hydrocarbons ; terpenoids ; eggs ; deterrents ; ants ; predation ; Chrysomelidae ; Coleoptera ; Gastrophysa cyanea ; reflex bleeding ; elytral glands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Egg clusters and adults ofGastrophysa cyanea are conspicuous and, like their larvae, are chemically protected. The eggs owe their bright yellow color primarily to β-carotene and, in addition, contain substantial quantities of oleic acid. At natural concentrations oleic acid effectively deters many species of ants from feeding. The use of fatty acids as deterrents against ants is discussed as a possible widespread phenomenon among insects. During defensive confrontations, adults ofG. cyanea exhibit avoidance behavior and may also feign death. In addition, the adults may autohemmorhage or secrete a fluid from elytral or pronotal pores in response to traumatic stimuli. The secretions are effective against ants and contain a mixture of hydrocarbons as well as terpenoid components. The pattern of ontogenetic modification in the defensive chemical repertoire ofG. cyanea is discussed.
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  • 47
    ISSN: 1573-1561
    Keywords: Scolytus scolytus ; Coleoptera ; Scolytidae ; pheromone ; 4-methyl-3-heptanol stereoisomer ; olfaction ; electrophysiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electroantennogram (EAG) and single-cell recording techniques have been used to demonstrate the presence of separate receptors for (−)-threo- and (−)-erythro-4-methyl-3-heptanol on the antenna ofS. scolytus. The majority of single-cell recordings showed spikes of two different amplitudes. The cell giving spikes of larger amplitude responded to the (−)-threo stereoisomer while the cell with the small-amplitude spikes responded to (−)-erythro-4-methyl-3-heptanol. It is suggested that in most recordings the two cells are associated with a single sensillum basiconicum.
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    Journal of chemical ecology 8 (1982), S. 493-506 
    ISSN: 1573-1561
    Keywords: Choristoneurafumiferana (Clem.) ; Lepidoptera ; Tortricidae ; sex pheromone ; mating disruption ; sex attraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Male spruce budworm [Chorisloneura fumiferana (Clem.)] moths were held for 3 hr in a wind tunnel and subjected to various concentrations of background synthetic pheromone. They were then exposed to calling females and their response was recorded. The background pheromone was presented either as discrete turbulent plumes or as a uniform permeation throughout the tunnel. The numbers of males wing-fanning and flying in response to the calling females decreased as the concentration of background pheromone increased. Of the males which flew, a higher proportion progressed upwind in the discrete plumes than in the uniform permeation, an indication that structure in the pheromone cloud is necessary for upwind progression. In both discrete plumes and uniform permeation fewer males were able to locate the females (i.e., disruption was greater) as the concentration of synthetic pheromone increased, but for the same total release rates, disruption was greater when the synthetic pheromone was released in discrete plumes rather than in a uniform permeation. This implies that disruption which involves luring males to sources of synthetic pheromone is more effective than masking female plumes by uniform permeation and suggests that it is more efficient to release pheromone from a few potent sources than from numerous low-potency sources.
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  • 49
    ISSN: 1573-1561
    Keywords: Dendroctonus frontalis ; Coleoptera ; Scolytidae ; seasonal variation ; pheromone content ; environmental parameters
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    Notes: Abstract The response ofDendroctonus frontalis to an attractant mixture (frontalin,trans-verbenol, and loblolly pine turpentine) was measured in the laboratory over a four-year period. Beetle response was highest in late winter and early spring, and lowest in midsummer and early fall. Males consistently responded higher than females. Female beetles displayed significantly higher responses in early morning and late afternoon than in the middle of the day. Analysis of beetle pronotal width and fat content revealed a high degree of correlation between these two parameters in female beetles, but there was no correlation of response with either fat content or pronotal width for either sex. There was no evident relationship between mean monthly beetle response and total amounts of frontalin andtrans-veibenol found in hindgut extracts. Daily temperature in months both during which beetles were bioassayed and immediately prior to bioassay was highly correlated to response to the attractant.
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  • 50
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    Journal of chemical ecology 8 (1982), S. 671-678 
    ISSN: 1573-1561
    Keywords: Bembidion obtusidens ; Coleoptera ; Carabidae ; Oscillatoria animalis ; Oscillatoria subbrevis ; Cyanophyta ; metabolites ; volatiles ; ecophenes ; methylhexadecanoate ; methyloctadecenoate ; attractant ; habitat selection
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    Notes: Abstract In laboratory bioassaysBembidion obtusidens Fall adults were attracted to volatile metabolites (methyl esters of hexadecanoic and 9-octadecenoic acid) of mat-forming, filamentous blue-green algae (Oscilalatoria sp.; Cyanophyta) growing in the microhabitat of these beetles on the shores of saline lakes. Commercial preparations of these metabolites also were attractive, suggesting thatOscillatoria metabolites are token stimuli which serve as habitat cues forBembidion adults.
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  • 51
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    Journal of chemical ecology 8 (1982), S. 701-707 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; Dendroctonus ponderosae ; pheromone ; exo-brevicomin ; endo-brevicomin ; Pinus contorta
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Bothexo- andendo-brevicomin reduced the response of flyingDendroctonus ponderosae (Coleoptera: Scolytidae) to an attractant composed oftrans-verbenol and terpenes or oftrans- andcis-verbenol and terpenes in a stand ofPinus contorta var.murrayana. These data suggest that racemicexo- andendo-brevicomin may interrupt aggregation in populations of mountain pine beetle colonizing lodgepole pine; functions of the natural chiral compounds are unknown.
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  • 52
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    Journal of chemical ecology 8 (1982), S. 731-754 
    ISSN: 1573-1561
    Keywords: Alkenylcompounds ; pheromones ; chemotaxonomy ; decenyl dodecenyl ; tetradecenyl ; hexadecenyl ; trapping ; Lepidoptera ; Noctuidae
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Sex attractants known for 145 species of noctuid moths have many common features both as to chemical constituents and to their relationships in blends. The great majority of constituents are straight-chain (Z)-alkenols, -alkenals, or -alkenyl acetates of even carbon number (10 through 16). The unsaturation is nonterminal in odd-numbered positions (5 through 11). In effective lures, these components are blended in specific ratios and the components in a sex pheromone or sex attractant blend are structurally related by “one-change” steps. This means that any blend component differs from one or more other components by a single structural alteration, such as a change in double bond position, or a change in carbon chain length, or a change in the oxygen function. For the few multicomponent systems known in detail, the central place in the “one-change” framework is occupied by the predominant blend component. Different patterns of occurrence of lure components occur in the subfamilies Acronictinae, Noctuinae, Hadeninae, Cuculliinae, Amphipyrinae, Heliothidinae, Plusiinae, Acontiinae, and Pantheinae, and some subfamilies are as yet without known lures. Some guiding principles for elucidation of blend compositions for unstudied species are presented; these guidelines can also be used in improvement of some synthetic blends of unsatisfactory quality.
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  • 53
    ISSN: 1573-1561
    Keywords: Monarch butterfly ; Asclepias spp. ; monooxygenase ; induction ; cardenolide ; aldrin epoxidation ; p-chloro-N-methylanilme demethylation ; Lepidoptera ; Danaidae
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Monarch butterfly larvae were examined for NADPH-dependent monooxygenase activities. Midgut and fat body homogenates catalyzed aldrin epoxidation andp-chloro-N-methylanilineN-demethylation at consistently low rates compared to many other lepidopteran larvae. Homogenates from larvae collected from four different milkweeds (Asclepias spp.) with quite different cardenolide contents had similar levels of activity. There were no detectable variations in activity due to season or year of collection.
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  • 54
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    Journal of chemical ecology 8 (1982), S. 837-842 
    ISSN: 1573-1561
    Keywords: 2-Tridecanone ; structure-activity relationships ; Lycopersicon hirsutum f.glabratum C.M. Mull ; Heliothis zea (Boddie) ; Lepidoptera ; Noctuidae ; resistance
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    Notes: Abstract Toxicity bioassays of the naturally occurring insecticide 2-tridecanone and a group of structural analogs against the tomato fruitworm (Heliothis zea) reveal a significant influence of chain length on toxicity of methyl ketones. This effect may be due to the differential ability of these compounds to penetrate lipid barriers and reach the active site, since a close relationship is seen between toxicity and lipophilicity. Congeners of chain lenths 15 and over are less active than predicted by this relationship, possibly due to steric effects.
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  • 55
    ISSN: 1573-1561
    Keywords: Enantiomers ; bark beetle ; pheromone ; Dendroctonus frontalis ; Coleoptera ; Scolytidae ; southern pine beetle ; electrophysiology ; olfaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In laboratory and field bioassays, the response ofDendroctonus frontalis was significantly greater to the mixture of (1S, 5R)-(−)-frontalin andalpha-pinene than to (1R,5S)-(+)-frontalin andalpfa-pinene. Electro-physiological studies revealed that antennal olfactory receptor cells were significantly more responsive to (1S, 5R)-(−)-frontalin than to (1R, 5S)-(+)-frontalin. Both enantiomers stimulated the same olfactory cells which suggests that each cell possesses at least two types of enantiomer-specific acceptors.
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  • 56
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    Journal of chemical ecology 8 (1982), S. 1125-1132 
    ISSN: 1573-1561
    Keywords: Peachtree borer ; Synanthedon exitiosa (Say) ; Lepidoptera ; Sessiidae ; oviposition stimulant ; peach ; Prunus persica
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Peachtree borer (PTB) cocoons, peach tree bark, and a mixture of frass and gum obtained arount PTB wounds were extracted with organic solvents of increasing polarity. Female PTB moths,Synanthedon exitiosa (Say), laid the greatest number of eggs on filter paper substrates treated with acetone extracts of PTB cocoons, ethyl acetate extracts of peach tree bark and acetone extracts of frass and gum mixtures. The extracts of cocoons or frass and gum mixture were separated by liquid chromatography and thin layer chromatography.
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  • 57
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    Journal of chemical ecology 8 (1982), S. 1183-1191 
    ISSN: 1573-1561
    Keywords: Kairomone ; contact chemicals ; arrestant ; host seeking ; Eucelatoria bryani ; Diptera ; Tachinidae ; Heliothis ; Lepidoptera ; Noctuidae ; parasitoid
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Factors acting at contact or close range affected the behavior associated with host seeking of females, but not of males, of the tachinid,Eucelatoria bryani Sabrosky. Females were arrested by components of larvae ofHeliothis virescens (F.) and by a dichloromethane extract of okra leaves. A hexane extract ofH. virescens frass and a chloroform-methanol extract ofH. virescens larvae were both active. In addition to chemicals, shape and size were involved because females were arrested by small inert objects the size ofH. virescens larvae and frass.
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  • 58
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    Journal of chemical ecology 8 (1982), S. 1455-1462 
    ISSN: 1573-1561
    Keywords: Efficacy ; electroantennogram ; looplure ; response threshold ; sex attractant ; trapping ; Trichoplusia ni ; Pseudoplusia includens ; Rachiplusia ou ; pheromone ; Lepidoptera ; Noctuidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Source concentration differences of (Z)-7-dodecen-1-ol acetate, or looplure, were evaluated for field trapping efficiency and electrophysiological responses with malePseudoplusia includens (Walker),Trichoplusia ni (Hubner) andRachiplusia ou (Guenné) (Lepidoptera: Noctuidae). Sticky traps baited with 1000 μg of the lure captured a significantly greater (P 〈 0.05) number of maleP. includens andT. ni than any other concentration;R. ou males were caught at a greater rate in traps baited with 100 μg of looplure, significantly more (P 〈 0.05) than with 1000 μg. Electroantennogram (EAG) studies demonstrated that antennae of maleP. includens have a lower response threshold to looplure than eitherT. ni orR. ou antennae, the latter demonstrating the highest significant threshold of response. No differences in the stimulus-response functions of the three species were detected.
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  • 59
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    Journal of chemical ecology 8 (1982), S. 363-371 
    ISSN: 1573-1561
    Keywords: Dendroctonus brevicomis ; Coleoptera ; Scolytidae ; Pinus ponderosa ; pheromone biosynthesis ; bark beetle ; myrcene ; ipsdienol ; exo-brevicomin ; frontalin ; attractants ; pheromones ; competition
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    Notes: Abstract When both sexes of the bark beetle,Dendroctonus brevicomis LeConte, were exposed to vapors of myrcene from ponderosa pine, only the male produced (+)-ipsdienol. In the field, racemic ipsdienol significantly reduced the attraction of both sexes in flight to a mixture of myrcene and the aggregation pheromones,exo-brevicomin and frontalin. This suggests that ipsdienol may be involved in regulating colonization density ofD. brevicomis. The implications of the biosynthesis of various enantiomers of ipsdienol byD. brevicomis and the cohabitating bark beetles,Ips paraconfusus andI. pini, in relation to their behavioral responses are discussed in regard to reducing interspecific competition.
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  • 60
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    Journal of chemical ecology 8 (1982), S. 383-396 
    ISSN: 1573-1561
    Keywords: Insect sex pheromone ; fall webworm moth ; American white moth ; Hyphantria cunea ; salt march caterpillar moth ; Estigmene acrea ; Lepidoptera ; Arctiidae ; (Z,Z)-9,12-octadecadienal ; linolealdehyde ; (Z,Z,Z)-9,12,15-octadecatrienal ; linolenaldehyde ; (Z,Z)-3,6-cis-9,10-epoxyheneicosadiene
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    Notes: Abstract Three compounds have been identified as sex pheromone components produced by female fall webworm moths,Hyphantria cunea (Drury). These compounds are: (Z,Z)-9,12-octadecadienal (I), (Z,Z,Z)-9,12,15-octadecatrienal (II), and (Z,Z)-3,6-cis-9,10-epoxyheneicosadiene (III). The ratio of these compounds was approximately 5∶6∶13, respectively, in female tip extracts prepared from U.S.S.R, insects and approximately 1∶8∶21, respectively, in extracts from U.S. insects. The ratio in female effluvia trapped from U.S. insects was 1∶6∶27, respectively. Compound III plus either I or II is effective in eliciting upwind flight in a wind tunnel. Compounds I, II, and III are also components of the sex pheromone system of the saltmarsh caterpillar moth,Estigmene acrea (Drury).
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  • 61
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    Journal of chemical ecology 8 (1982), S. 397-408 
    ISSN: 1573-1561
    Keywords: Pea moth ; Cydia nigricana (F.) ; Lepidoptera ; Olethreutidae ; sex attractant ; (E)-10-dodecen-1-yl acetate ; (E,E)-8,10-dodecadien-1-yl acetate ; inhibitor ; Synergist ; lure
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    Notes: Abstract (E)-10-Dodecen-1-yl acetate (E10–12∶Ac) and (E,E)-8,10-dodecadien-1-ylacetate (E,E8,10–12∶Ac) are sex attractants for males of the pea moth,Cydia nigricana (F.). Thirty-two structurally related compounds with chain lengths of 9–14 carbon atoms were exposed withE10–12∶Ac orE,E8,10–12∶Ac in traps in the field to investigate their influence on the activity of the attractants. Only alchols and acetates unsaturated at C-8, -9, or -10 greatly influenced moth captures. (Z) and (E)-8-dodecen-1-ol were weak synergists forE10–12∶Ac but no synergists forE,E8,10–12∶Ac were found. (Z) and (E)-8-dodecen-1-yl acetate and (E,E)-8,10-dodecadien-1-ol inhibited bothE10–12∶Ac andE,E8,10–12∶Ac while (E)-10-dodecen-1-ol, 10-dodecyn-1-ol, (Z) and (E)-9-dodecen-1-yl acetate, (Z)-10-dodecen-1-yl acetate, and undecyl acetate inhibited only the former attractant.
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  • 62
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    Journal of chemical ecology 8 (1982), S. 507-515 
    ISSN: 1573-1561
    Keywords: Almond moth ; Ephestia cautella ; Lepidoptera ; Pyralidae ; stored products ; pest management ; behavior ; density ; flight
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    Notes: Abstract The mating rate ofEphestia cautella (Walker) was inversely related to the level of pheromone permeating the air. However, permeation did not prevent mating by altering the number of females calling or the frequency, duration, or pattern of male flight. Instead, permeation probably prevented mating by reducing the distance from which a male can respond to a female. The pheromone level necessary to achieve a particular reduction in mating increases with moth density.
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  • 63
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    Journal of chemical ecology 8 (1982), S. 527-534 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Bruchidae ; Callosobruchus maculatus ; cowpea weevil ; pheromone ; sex attractant
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    Notes: Abstract Female cowpea weevils,Callosobruchus maculatus (F.), emitted a pheromone which excited males. Pheromone release began soon after emergence and continued for one week. Synchronization of pheromone release with calling behavior was demonstrated. Mating reduced pheromone release but not male response. Pheromone obtained by aeration collection was utilized for determining a quantitative dose-response relationship.
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  • 64
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    Journal of chemical ecology 8 (1982), S. 653-661 
    ISSN: 1573-1561
    Keywords: Sex pheromone ; aphrodisiac ; odd beetle ; Thylodrias contractus ; Motschulsky ; Coleoptera ; Dermestidae ; bioassay ; behavior ; exocrine gland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Adult virgin females of the odd beetle,Thylodrias contractus, produce a volatile sex pheromone that influences the behavior of conspecific males and attracts them in laboratory olfactometer tests. The active substance can be collected by allowing virgin females to walk on absorbent paper disks. Disks exposed to mated females do not elicit a response from males. The male odd beetle repeatedly rubs the antennae, head, and thorax of a female with a setate glandular area on his second abdominal sternum during courtship, probably secreting a reciprocally active aphrodisiac substance.
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  • 65
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    Journal of chemical ecology 8 (1982), S. 663-670 
    ISSN: 1573-1561
    Keywords: Pandemis heparana ; sex pheromone ; Lepidoptera ; Tortricidae ; gas chromatography ; mass spectrometry ; insect behavior ; (Z)-11-tetradecen-1-yl acetate ; (Z)-9-tetradecen-1-yl acetate ; (Z)-11-tetradecenl-ol ; tetradecyl acetate ; dodecyl acetate
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    Notes: Abstract 90% (Z)-11-tetradecen-1-yl acetate, 5% (Z)-9-tetradecen-1-yl acetate, 5% (Z)-11-tetradecen-1-ol, dodecyl acetate (〈1%), and tetradecyl acetate (〈1%) were identified from gland extracts ofPandemis heparana females by gas chromatography and gas chromatography-mass spectrometry analysis (including mass fragmentography studies), chemical characterization, total synthesis, laboratory and field bioassays. In the field, a mixture of (Z)-11-tetradecen-l-yl acetate and (Z)-9-tetradecen-l-yl acetate (95∶5) was found to be essential for attractiveness ofP. heparana males.
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  • 66
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    Journal of chemical ecology 8 (1982), S. 679-687 
    ISSN: 1573-1561
    Keywords: Sitophilus granarius ; Coleoptera ; Curculionidae ; aggregation pheromone ; granary weevil
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Evidence for a male-produced aggregation pheromone inSitophilus granarius is reported. Hexane extracts of Tenax®-trapped volatiles from males held on wheat were attractive to both sexes in a multiple-choice olfactometer and pitfall bioassay chamber. A quantitative relationship existed between days of insect exposure on disks and degree of responsiveness. The maximum response was to 35 insect-day-equivalents. Diel-related activity showed both sexes responsive during photophase and nonresponsive during scotophase.
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  • 67
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    Journal of chemical ecology 8 (1982), S. 755-762 
    ISSN: 1573-1561
    Keywords: Sex pheromone ; Heliothis virescens ; tobacco budworm ; Heliothis zea ; bollworm ; virelure ; Lepidoptera ; Noctuidae
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract None of the isolated degradation products of (Z)-11-hexadecenal [(Z)-11-HDAL] affected the catches of either tobacco budworm [Heliothis virescens (F.)] or bollworm [H. zea (Boddie)] moths when dispensed with pheromone from cotton dental rolls in cone traps. Also, none of the degradation products of (Z)-9-tetradecenal [(Z)-9-TDAL] had an effect on trap catches of tobacco budworm moths. Two of the three chemicals that have previously been identified in ovipositor washes of tobacco budworms but that are absent in those of bollworms caused a reduction in capture of bollworms: (Z)-9-TDAL (1.0 μg/trap) caused a 96% reduction in trap catch and (Z)-11-hexadecen-1-ol (20.0 μg/trap) caused a similar reduction. Tetradencenal (40 μg/trap) had no effect on trap catch.
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  • 68
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    Journal of chemical ecology 8 (1982), S. 773-783 
    ISSN: 1573-1561
    Keywords: Aleochara curtula (Goeze) ; Coleoptera ; Staphylinidae ; tergal gland secretion ; hydrocarbons ; aliphatic aldehydes ; substituted 1 ; 4-benzoquinones ; n-undecane ; 1-undecene ; (Z)-4-tridecene ; n-dodecane ; (Z)-5-tetradecenal ; toluquinone ; 2-methoxy-3-methyl-1 ; 4-benzoquinone
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    Notes: Abstract The defensive secretion from the tergal gland of the staphylinid beetle,Aleochara curtula, acting as a supplementary mating stimulant, was investigated by gas-liquid chromatography, mass spectrometry, and IR spectroscopy. The reservoir contains a complex mixture of hydrocarbons, aliphatic aldehydes, and substituted 1,4-benzoquinones.n-Undecane, 1-undecene, and (Z)-4-tridecene were identified as the major hydrocarbon components. The main aldehydes aren-dodecanal and (Z)-5-tetradecenal, and the chief quinones are toluquinone and 2-methoxy-3-methyl-1,4-benzoquinone, the latter being established structurally by comparison of the mass spectra of the three synthesized isomers. Quantitative GLC analyses revealed no sex specificity of the relative concentrations of the compounds.
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  • 69
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    Journal of chemical ecology 8 (1982), S. 843-850 
    ISSN: 1573-1561
    Keywords: Kairomones ; 2-acylcyclohexane-1 ; 3-diones ; Ephestia keuhniella Zeller ; (syn.Anagasta kuehniella Zeller) ; Lepidoptera ; Pyralidae ; Nemeritis canescens (Grav.) (syn.Venturia canescens (Grav.)) ; Ephestia cautella (Wlk.) ; Ephestia elutella (Hubner) ; Hymenoptera ; Ichneumonidae ; Plodia interpunctella (Hubner) ; oviposition
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    Notes: Abstract 2-Acylcyclohexane-1,3-diones, a novel class of compounds in the larval mandibular glands ofEphestia(=Anagasta) kuehniella Zeller, act as kairomones. Their relative activities in eliciting oviposition responses from the larval parasiteNemeritis(=Venturia) canescens (Grav.) are reported.
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  • 70
    ISSN: 1573-1561
    Keywords: Antennae ; esterases ; pheromone degradation ; cuticle ; olfaction ; cabbage looper ; Trichoplusia ni ; Lepidoptera ; Noctuidae
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Examination was made of the hydrolytic activities of esterases obtained from the antennae, legs, and wings of 3-day-old cabbage looper moths,Trichoplusia ni (Hübner), by elution and by homogenation of those appendages. Pheromone hydrolysis in 1-min assays was monitored by use of tritium-labeled (Z)-7-dodecen-1-ol acetate and thin-layer chromatography to separate the reaction products. Listed according to the activities of the esterases obtained by homogenation, the organs were antennae 〉 legs 〉 wings. In contrast, the order according to the activities of the eluted esterases was wings 〉 legs 〉 antennae. Also, the eluted enzymes were less active than the esterases obtained by homogenization. The relatively high pheromone-hydrolyzing activity present in homogenized antennae suggests that the esterases originated inside the antennae and lends support to the hypothesis proposed in earlier investigations that pheromone-inactivating enzymes may play an important role in the olfactory process, possibly by clearing pheromone from the vicinity of the olfactory receptors. The esterases detected on the cuticle, on the other hand, may function by preventing surface accumulation of pheromone. The higher measured esterase activity in homogenates of prothoracic legs than of mesothoracic or metathoracic legs suggests that the prothoracic legs, which are used to clean the antennae of debris, may function by removing and degrading pheromone from the surface of antennae.
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  • 71
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    Journal of chemical ecology 8 (1982), S. 923-933 
    ISSN: 1573-1561
    Keywords: Aldehyde pheromone ; bioluminescence ; corn earworm ; Heliothis zea ; insect pheromone ; navel orangeworm ; Amyelois transitera ; western spruce budworm ; Choristoneura occidentalis ; spruce budworm ; Choristoneura fumiferana ; Lepidoptera ; assay for aldehydes ; Pyralidae ; Noctuidae ; Tortricidae
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    Notes: Abstract Pheromone levels in the glands of individual female moths of the spruce budworm (Choristoneura fumiferana), the western spruce budworm (C. occidentalis), the navel orangeworm (Amyelois transitella), and the corn earworm (Heliothis zea) were quantitively measured by means of a new bacterial bioluminescence assay specific for aldehydes. The sensitivity and rapidity of the bioluminescent assay enabled studies to be conducted on the dependence of the pheromone levels in the spruce budworm on age and the effect of photoperiod on the pheromone levels in the corn earworm. The bioluminescence assay provides a rapid and sensitive approach for studying aldehyde pheromone levels and their regulation in insects.
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    Journal of chemical ecology 8 (1982), S. 935-945 
    ISSN: 1573-1561
    Keywords: Aldehydes ; bioluminescence ; insect pheromones ; Porapak Q ; spruce budworm ; Choristoneura fumiferana ; Lepidoptera ; (E)-11-tetradecenal ; trapping ; bioassay for aldehydes
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    Notes: Abstract A newly developed bioluminescent assay was used to measure quantitatively the amount of (E)-11-tetradecenal, the major component of the sex pheromone of the spruce budworm, trapped on Porapak Q®. The bioluminescent response was linearly related to the amount of aldehyde either deposited on the absorbent or trapped from an airstream. However, the recovery of pheromone from Porapak was dependent on whether the air was prefiltered (through Porapak) or taken directly from the atmosphere. Furthermore, pheromone on Porapak was lost with time during the flow of air through the absorbent, indicating that trapping of aldehyde pheromone should be conducted for short periods of time for optimal recoveries. The applicability of the assay system for the rapid and direct measurement of the release rates of aldehyde pheromone lures was demonstrated for pheromone lures used for baiting spruce budworm traps.
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  • 73
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    Journal of chemical ecology 8 (1982), S. 973-980 
    ISSN: 1573-1561
    Keywords: (E, Z)-7,9-Dodecadien-1-yl acetate ; European grapevine moth ; Lobesia botrana ; Lepidoptera ; Tortricidae ; sex pheromone ; photoisomerization ; conjugated diene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Photo- and radical isomerization of (E, Z)-7,9-dodecadien-1-yl acetate (DDA) leads to an equilibrium mixture of all four possible geometric isomers of 7,9-DDA in the ratio ofE,E, 69–76%;Z,E, 11–13%;E, Z, 12–15%; andZ, Z, 1–3%. Iodine catalysis of the isomerization takes place even in dark at room temperature and is probably a radical reaction.
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  • 74
    ISSN: 1573-1561
    Keywords: Heliothis virescens ; Lepidoptera ; Noctuidae ; Z11-16:Ald ; 16:Ald ; Z9-14:Ald ; 14:Ald ; sex pheromone emission ; gland volatiles ; blend composition ; pheromone emission rates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Sex pheromone gland volatiles from individualHeliothis virescens (F.) females were collected and analyzed on an SP-2330 capillary gas-liquid chromatography column for identification and quantification of the compounds emitted. Only four of the seven compounds previously reported as pheromone components appeared consistently in the volatile collections: 14:Ald, Z9-14:Ald, 16:Ald, and Z11-16:Ald. The female glands did not emit the same amounts of these compounds throughout a 24-hr period; they emitted maximum quantities between 6 and 11 hr after the onset of scotophase with the remainder of the photoperiod having minimal emission rates. Although the absolute quantities fluctuated, the percent compositions of the compounds remained about the same throughout the 24-hr period.
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  • 75
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 8 (1982), S. 1093-1109 
    ISSN: 1573-1561
    Keywords: Scolytidae ; Coleoptera ; conifers ; terpenoids ; olfactory tests ; host attraction specificity ; statistical methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Nine Scolytidae (Cryphalus piceae, Cryphalus abietis, Pityokteines curvidens, Dendroctonus micans, Ips sexdentatus, Ips typographus,Orthotomicus erosus, Tomicus piniperda, andPhloeosinus bicolor) were subjected to olfaction tests on ten conifer species taken two by two. These conifers wereAbies cephalonica, Abies nordmanniana, Picea abies, Picea orientalis, Pinus pinaster, Pinus sylvestris, Pinus brutia, Pinus laricio,Cupressus atlantica, andCupressus sempervirens. A statistical study of the results, by means of the factorial analysis of correspondence completed by the duo preference test, commonly used in sensory analysis, revealed a taxonomic clustering by genus of the plant species and analogous specific attraction behavior for the insects.Pityokteines curvidens has a behavior analogous to that of the twoCryphalus considered.Phloesinus bicolor shows a very strong specificity forCupressus. The essential oils of the conifers were analyzed to determine their terpene composition and the ten odor spectra thus obtained were compared. The hierarchical classification, using a Euclidian distance, brought out similarities in the spectra, especially in the case ofPinus. It is shown that definitive establishment of Scolytidae is not due to the presence in the odor spectrum of any particular terpenoid. The attractive power of a species results from the synergism of the different terpenes. Moreover the definitive establishment of the insects also depends on their sensorial adaptation to volatile substances which can be wider or narrower for the species studied.
    Type of Medium: Electronic Resource
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