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  • Artikel  (56)
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  • Springer  (56)
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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 151-156 
    ISSN: 1570-7458
    Schlagwort(e): Xyleborus ferrugineus ; pupae ; ecdysteroids ; pharate adult ; radioimmunoassay ; Coleoptera ; Scolytidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Der Ecdysteroidtiter weiblicher Puppen von Xyleborus ferrugineus (Fabr.) wurde geschätzt, indem ganze Tiere homogenisiert und radioimmunologisch untersucht wurden. Ein ausgeprägtes Maximum an Ecdysteroiden wurde bei 36 Stunden Puppenent-wicklung beobachtet (743 pg/mg Körpergewicht). Der Titer nahm ab auf 299 pg/mg im Pharatstadium und auf 193 pg/mg unmittelbar vor Schlüpfen der Adulten. Qualitative Studien mit HPLC ergaben in frischen Puppen ein Verhältnis von 3:1 Ecdyson zu 20-Hydrooxyecdyson. Pharatstadien enthielten vor allem 20-Hydrooxyecdyson. Das beobachtete einzige Maximum im Titer stimmt überein mit den Resultaten bei andern untersuchten Coleopteren.
    Notizen: Abstract Ecdysteroid titers were estimated on the whole body homogenates of Xyleborus ferrugineus (Fabr.) female pupae during development by radioimmunoassay. A distinct peak of ecdysteroids was observed at 36-hr pupal development (743 pg/mg body wt). Titer declined to 299 pg/mg by the pharate adult stage and to 193 pg/mg body wt just before adult emergence. Qualitative studies by HPLC revealed a ratio of 3:1 ecdysone to 20-hydroxyecdysone in the initial pupal stage. Pharate adults had mainly 20-hydroxyecdysone. The observed single peak in ecdysteroid titer agrees with findings in other studied coleopteran species.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    ISSN: 1432-041X
    Schlagwort(e): Mollusca ; Cleavage ; Junctions ; Interaction ; Development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In early cleavage stages ofLymnaea stagnalis, three kinds of intercellular junctions could be distinguished up to the sixth cleavage: intermediate, septate and gap junctions. The first two form “junctional belts” located on the cell border at the periphery of the embryo. For the purpose of our study we were most interested in gap junctions as they are alleged to be structures that allow cell-to-cell communication. Gap junctions first appear at the four cell stage. Up to the sixth cleavage no difference in the distribution pattern could be found between and within each of the four quadrants of the embryo. Some of the cell tiers along the animal-vegetal axis lack gap junctions either between the blastomeres within the tier or between the blastomeres from adjacent tiers. All gap junctions observed in freeze fracture replicas show plaques with an irregular IMP pattern. The average IMP diameter measures 12 nm (SD±2 nm). In stages fixed after the fifth cleavage, gap junctions are found between micromeres at the animal pole and the central 3D macromere. This is in agreement with the presumed interaction between these cells at this stage. The possibility of a transition of non-functional into functional gap junctions after the fifth cleavage is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 190 (1981), S. 33-39 
    ISSN: 1432-041X
    Schlagwort(e): Ceratitis capitata ; Major haemolymph proteins ; Development ; Fat body ; Secretion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The accumulation of major haemolymph proteins (a group of proteins immunologically related to Calliphorin) their biosynthesis in vivo and in organ culture as well as their secretion, has been studied during the late larval stages and white pupae of the Mediterranean fruit flyCeratitis capitata. The accumulation of major haemolymph proteins in the haemolymph, shows a twenty fold increase from the 4-day old larvae to the white pupae stage, while in the fat body there is only a seven fold increase. It is evident from the in vivo and organ culture studies, that the major haemolymph proteins are synthesized during the late larval stage and their synthesis declines abruptly during the stage of white pupae. It seems also that each polypeptide has its own characteristic developmental kinetics of synthesis. The major haemolymph proteins are synthesized in the fat body and are very quickly secreted into the haemolymph.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Planta 151 (1981), S. 15-25 
    ISSN: 1432-2048
    Schlagwort(e): Auxin (uptake, transport) ; Benzoic acid ; N-1-Naphthylphthalamic acid ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The validity of a chemiosmotic hypothesis for uptake of weak acids as an explanation for the accumulation of auxin by cells has been explored further by comparing the uptake of indole-3-acetic acid (IAA) by 1-mm segments of corn (Zea mays L.) coleoptiles with that of benzoic acid and two neutral indoles, indoleethanol and indoleacetonitrile, which do not ionize. These substances, while structurally related to IAA lack both auxin activity and polar transport. Uptake of IAA and benzoic acid increase with decreasing external pH, whereas the uptake of the two neutral indoles is independent of external pH. Although metabolism of IAA, during 90 min or less, is minimal and without significant effect on its uptake, metabolism of benzoic acid appears responsible for the apparent saturation of benzoic acid uptake at high concentrations. An inhibitor of auxin transport, N-1-naphthylphathalamic acid (NPA), stimulates uptake of IAA but has no effect on uptake of either benzoic acid or the two neutral indoles. Thus, NPA does not affect the driving forces for accumulation of weak acids but probably specifically decreases the flux of the auxin anions relative to undissociated auxin. Since the electrochemical potential of auxin anions is usually higher in than outside cells, blocking the anion flux with NPA would enhance auxin uptake. Azide, which abolishes accumulation of both IAA and benzoic acid, may simply collapse the pH gradient across the plasma membrane. In the absence of NPA, increasing concentrations of auxins or the analogoue β-naphthaleneacetic acid (β-NAA) exert two opposing effects on the uptake of IAA-depression and stimulation. Stimulation results from saturating the anion flux. With uptake fully stimulated by NPA, however, increasing concentrations of auxins or analogues only depress uptake of [3H]IAA. These results are consistent with more than one path for auxin transport each with a different dependence on concentration. In depressing NPA-stimulated IAA uptake, the effectiveness of β-NAA≧IAA≫α-NAA≫ benzoic acid, a specificity similar to that of an auxin binding site in vitro that has been implicated by others in auxin transport. The results support the general hypothesis that cellular auxin uptake and polar transport through tissues are chemiosmotically coupled to the electrochemical potential of auxin and protons.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Planta 153 (1981), S. 436-442 
    ISSN: 1432-2048
    Schlagwort(e): Aminopterin-resistant variants ; Cell culture ; Folate analog ; Variant selection ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Aminopterin-resistant cell lines of maize were isolated by two different procedures of callus selection and by plating suspension cultures on drugcontaining medium after mutagen treatment. Efficiencies of different methods of variant selection were compared. Four aminopterin-resistant cell lines were shown to be 10–40 times more resistant than the parental cell line, and they were also resistant to another folate analog, methotrexate. The results suggest that alterations in at least three different cell properties could be responsible for resistance; 1) increased dihydrofolate reductase activity, 2) altered aminopterin sensitivity of dihydrofolate reductase, and 3) reduced drug uptake. One of the resistant cell lines showed more than one alteration, but its resistance proved to be unstable. The results suggest that stable changes which may or may not be of genetic origin and also unstable physiological changes or a combination of both could lead to aminopterin resistance in maize cell cultures.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Planta 153 (1981), S. 443-446 
    ISSN: 1432-2048
    Schlagwort(e): ATPase ; Mitochondria ; Mycotoxin ; Pisum ; Zea ; Zearalenone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract At 5 and 10 μg ml-1 concentration, zearalenone (F-2), a mycotoxin produced by a number of species of the genus Fusarium, causes an inhibition of the oxidative phosphorylation of isolated plant mitochondria, while at 20 and 40 μg ml-1 it causes uncoupling. However, when the mitochondria are pre-incubated for 20 min with F-2, the uncoupling appears to be the prevailing effect. F-2 is also able to inhibit the mitochondrial ATPase activity (Mg2+-dependent). Conversely, F-2 (40 μg ml-1) does not alter the ATP level of maize roots and only slightly affects the ATPase activity of pea stem and maize root microsomal fractions. In addition, F-2 (10–40 μg ml-1) inhibits ATP synthesis catalyzed by rat liver mitochondria. It is suggested that the phytotoxicity of F-2, also known for its ability to collapse the transmembrane electric potential of maize roots, may be mainly linked to its ability to increase the proton permeability of the cell, similar to the common uncouplers.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Planta 153 (1981), S. 471-475 
    ISSN: 1432-2048
    Schlagwort(e): Abscisic acid ; Geotropism (root) ; Growth inhibitor ; Light and root-cap inhibitor ; Root cap (inhibitor) ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Growth inhibitors were assayed from extracts of intact (attached) and of excised (cultured) root caps of Zea mays L., cv. Merit, the roots of which show a positive geotropic response only after exposure to light. If caps are intact at the time of illumination, at least two inhibitory substances are produced, an acid inhibitor and a neutral inhibitor, whereas if caps are detached from roots, placed in culture and then illuminated only the neutral inhibitor is formed. Cycloheximide retards inhibitor production in both intact and cultured caps. When [14C]mevalonic acid is included in the culture medium and the caps are illuminated, 15–25% of the recoverable 14C cochromatographs with the neutral inhibitor, whereas in caps cultured in the dark, this radiolabelling pattern is not observed. Cyloheximide in the light reduces the incorporation of 14C into compounds cochromatographing at the Rf of the neutral inhibitor. It is suggested that the neutral inhibitor may be important in the light-induced bending of roots.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    ISSN: 1432-2048
    Schlagwort(e): Auxin (immunoassay) ; Avena ; Coleoptiles (physiological tip) ; Enzyme immunoassay ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A specific solid-phase enzyme immunoassay for the detection of as little as 3–4 pg of indole-3-acetic acid (IAA) is described. The assay involves minimal procedural efforts and requires only standard laboratory equipment. Up to 50 samples in triplicate, processed simultaneously, can be assayed and evaluated in 2.5 h. As little as 1 mg oat coleoptile tissue is sufficient for a quantitative IAA analysis and little or no extract purification is necessary. Using this assay, levels of IAA have been determined in coleoptiles of maize and oat. The distribution of IAA within single coleoptiles was quantitated and the production of IAA during the regeneration of the physiological tip in Avena coleoptiles was investigated. The changes in levels of IAA and other major phytohormones were quantitated during the growth of oat coleoptiles.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    ISSN: 1432-2048
    Schlagwort(e): Cell-wall autohydrolysis ; β-D-Glucan ; Glucanase ; Hemicellulose ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The proteins dissociated from isolated Zea seedling cell wall using high-ionic-strength salt solutions have been found to include a number of enzymes which appear to participate in autolytic reactions of the cell wall. These enzymes caused extensive degradation of enzymatically inactive cell wall, liberating as much as 100 μg/mg dry weight over a 48-h period. Lithium chloride (3M) was shown to be most effective in yielding protein and wall-degrading activities. Molecular-sieve chromatography of the cell-wall protein resolved endo-β-D-glucanase and exo-β-1,3-glucanase (EC 3.2.1.58) activities when Avena glucan and laminarin, respectively, were employed as substrates. The exoenzyme (molecular weight around 60,000) was strongly inhibited by inorganic mercury at a concentration which suppressed the release of monosaccharide from autolytically active cell wall. The endo-β-D-glucanase (MW around 26,000), which showed a marked preference for substrates of mixed-linkage, exhibited features indicating that it initiates the autolytic solubilization of wall glucan. Cell-wall β-D-glucan, recovered as a component of an alkali-soluble cell-wall fraction, served as a substrate for the purified glucanases. Their hydrolysis pattern, assessed using gel exclusion chromatography and product analysis, confirmed that they hydrolyze β-D-glucan. The products generated by the endoglucanase were similar in molecular-size distribution to those liberated from autolytically active-wall. Exoglucanase activity was required for extensive hydrolysis of β-D-glucan in vitro. During coleoptile development the autolytic activity of the cell wall increased dramatically. This increased activity, however, did not parallel the growth potential of the tissue, but more closely reflected an increase in cell-wall β-D-glucan, the primary substrate for autolytic reactions.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    ISSN: 1432-2048
    Schlagwort(e): Acid growth ; Auxin ; Growth ; Root ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The role of proton excretion in the growth of apical segments of maize roots has been examined. Growth is stimulated by acidic buffers and inhibited by neutral buffers. Organic buffers such as 2[N-morpholino] ethane sulphonic acid (MES) — 2-amino-2-(hydroxymethyl)propane-1,3 diol (Tris) are more effective than phosphate buffers in inhibiting growth. Fusicoccin(FC)-induced growth is also inhibited by neutral buffers. The antiauxins 4-chlorophenoxyisobutyric acid (PCIB) and 2-(naphthylmethylthio) propionic acid (NMSP) promote growth and H+-excretion over short time periods; this growth is also inhibited by neutral buffers. We conclude that growth of maize roots requires proton extrusion and that regulation of root growth by indol-3yl-acetic acid (IAA) may be mediated by control of this proton extrusion.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 11
    Digitale Medien
    Digitale Medien
    Springer
    Planta 151 (1981), S. 68-74 
    ISSN: 1432-2048
    Schlagwort(e): Energy metabolism ; Germination (seeds) ; Protein synthesis ; Temperature and seed germination ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Poor emergence of maize seedlings, due to high soil temperatures, is a major limitation of crop potential in the lowland tropics. Ability to germinate at high temperature (〉c. 37° C) is related to the temperature sensitivity of the embryo, and there is considerable genotypic variation for this character. Respiration and mitochondrial phosphorylation proceed normally in seeds imbibing at 41° C, and ATP levels are adequate for germination. However, the specific activities of several important enzymes are lower, and the rate of protein synthesis is severely reduced compared with seeds imbibing at 28° C. The depression of the rate of protein synthesis in the embryos of several tropical hybrids imbibing at high temperature correlated with their known temperature sensitivity. It is concluded that protein synthesis is an especially temperature sensitive process in germinating maize embryos, and that this is the principal reason for the sensitivity of germinating maize seeds to high temperature.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 12
    Digitale Medien
    Digitale Medien
    Springer
    Planta 151 (1981), S. 75-80 
    ISSN: 1432-2048
    Schlagwort(e): Embryos (protein synthesis) ; Germination (seeds) ; Protein synthesis and temperature ; Temperature and protein synthesis ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The poor germination of maize seeds at high temperatures (〉37°C) is related to the low rate of protein synthesis by the embryo. The apparatus of translation was not heat-labile when embryos were incubated for 2 h at 41°C, and cell free extracts from seeds imbibed for 16 h at this temperature were able to translate exogenous mRNA, indicating that ribosomes and other subcellular components were present and functional. Analysis of polysome profiles from embryos imbibing at high temperature indicated that the low rate of protein synthesis was due to the non-availability of active mRNA.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 13
    Digitale Medien
    Digitale Medien
    Springer
    Planta 151 (1981), S. 141-145 
    ISSN: 1432-2048
    Schlagwort(e): Chromosome isolation ; Petroselinum ; Polyethylene-glycol ; Protoplast (uptake of chromosomes) ; Triticum ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract For mass isolation of plant metaphase chromosomes, cultured cells of wheat (Triticum monococcum) and parsley (Petroselinum hortense) were synchronized by hydroxyurea and colchicine treatment. This synchronization procedure resulted in high mitotic synchrony, especially in suspension cultures of parsley in which 80% of the cells were found to be at the metaphase stage. Mitotic protoplasts isolated from these synchronized cell cultures served as a source for isolation of chromosomes. The described isolation and purification method yielded relatively pure chromosome suspension. The uptake of the isolated plant chromosomes into recipient wheat, parsley, and maize protoplasts was induced by polyethylene-glycol treatment. Cytological studies provided evidences for uptake of plant chromosomes into plant protoplasts.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 14
    Digitale Medien
    Digitale Medien
    Springer
    Planta 151 (1981), S. 146-150 
    ISSN: 1432-2048
    Schlagwort(e): Georeaction (root) ; Growth inhibitors ; Growth (root) ; Root (georeaction, growth) ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The growth rate of the two sides of 10-mm apical segments prepared from primary roots and of intact primary roots of maize has been analyzed in both vertical and horizontal positions, using a filming method allowing continuous growth recording. The data showed that the georeaction began by a decrease in the overall elongation rate of the roots. This inhibition is effective on the lower side of the bending zone, where the growth is practically stopped during the period of maximum rate of geocurvature. In contrast, the growth is slightly enhanced on the upper part of the elongating zone.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 15
    Digitale Medien
    Digitale Medien
    Springer
    Planta 152 (1981), S. 319-324 
    ISSN: 1432-2048
    Schlagwort(e): Nitrate influx (efflux, metabolism) ; Pennisetum ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Maize (Zea mays L.) and pearl millet (Pennisetum americanum (L.) Leeke) seedlings were exposed to [15N]nitrate for 1-h periods at eight times during a 24-h period (16–8 h light-dark for maize; 14–10 h for millet). Influx of [15N]nitrate as well as its reduction and translocation were determined during each period. The efflux of previously absorbed [14N]nitrate to the uptake solution was also estimated. No marked diurnal changes in [14N]nitrate efflux or [15N]nitrate influx were evident in maize. In contrast, [14N]nitrate efflux from millet increased and eventually exceeded [15N]nitrate influx during the late dark and early light periods, resulting in net nitrate efflux from the roots. The dissimilarity of their diurnal patterns indicates that influx and efflux are independently regulated. In both species, [15N]nitrate reduction and 15N translocation to shoots were curtailed more by darkness than was [15N]nitrate influx. In the light, maize reduced 15% and millet 24% of the incoming [15N]nitrate. In darkness, reduction dropped to 11 and 17%, respectively. Since the accumulation of reduced-15N in shoots declined abruptly in darkness, whereas that in roots was little affected, it is suggested that in darkness [15N]nitrate reduction occurred primarily in roots. The decrease in nitrate uptake and reduction in darkness was not related to efflux, which remained constant in maize and did not respond immediately to darkness in pearl millet.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 16
    Digitale Medien
    Digitale Medien
    Springer
    Planta 151 (1981), S. 499-505 
    ISSN: 1432-2048
    Schlagwort(e): Auxin ; Calorimetry ; Coleoptile ; Energetics ; Growth regulator ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Indole-3-acetic acid (IAA) promotes an increase in steady-state heat production by corn (Zea mays L.) coleoptile tissue; this increase is associated with an elevation in aerobic respiration rates. A detailed time dependence of the exothermic response to IAA was obtained using flow calorimetry. The latent period and magnitude of response were evaluated as a function of IAA concentration and pH. The data indicate that more than one response may occur. The optimal change in heat production was produced by an IAA concentration of 3·10-5 M. It was initiated within 5 min after the start of the IAA treatment, and reached a magnitude in excess of 25% of the tissue's basal heat production. Concentrations of IAA greater than 1·10-4 M resulted in diminished response(s), but the effect was strongly pH dependent. Several possibilities for the increased heat production triggered by IAA are discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 17
    Digitale Medien
    Digitale Medien
    Springer
    Planta 151 (1981), S. 506-511 
    ISSN: 1432-2048
    Schlagwort(e): Cell fractionation ; Cucurbita ; Malic enzyme ; Plasma membrane ; Subcellular localization ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A definite membrane fraction from Cucurbita hypocotyls, maize coleoptiles, and other plant tissues contains a NADP-dependent malic enzyme activity, up to 10% of overall tissue activity, and probably other soluble proteins. This “malic enzyme particle” is identified as plasmalemma on the basis of sedimentation behavior, density distribution in sucrose gradients, in comparison with enzyme markers, and sluggish penetration by the sugar Metrizamide. Enzyme binding to the plasma membrane is stable and scarcely sensitive to salts and EDTA, although all activity is released to the supernatant in the presence of Triton-X-100 or under hypotonic conditions. The properties of bound enzyme are similar to those of free enzyme in cell extracts. It is proposed that osmotically sensitive plasma membrane vesicles, containing cytoplasm fragments, are formed during homogenization. Low malic enzyme activities are also associated with Cucurbita proplastids.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 18
    ISSN: 1432-2048
    Schlagwort(e): Lycopersicon ; Phetorespiration ; Photosynthesis ; Triacontanol ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Tomato (C3-plants) and maize (C4-plants) were grown in a nutrient solution to which triacontanol was added twice a week. After about 4 weeks the triacontanol treatment caused a significant increase in the dry weight of the tomato plants. Leaf area and dry weight measurements of tomato leaves at different stages of development showed that the largest increase in growth was obtained when triacontanol treatment was initiated before bud formation. In maize, no effect of the triacontanol treatment on dry wieght was observed. Photosynthesis was inhibited by 27% in young leaves from triacontanol-treated tomato plants and 39% in the controls, when the oxygen concentration was raised from 2% to 21%. In maize no change in photosynthesis could be observed, neither after altered oxygen concentration nor after triacontanol treatment. The difference in the response of C3- and C4-plants to triacontanol indicates that it regulates processes related to photosynthesis.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 19
    ISSN: 1432-2048
    Schlagwort(e): Cytoplasmic pH ; Fusicoccin ; Hydrogen-ion extrusion ; pH-stat ; Root ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract 31P-Nuclear-magnetic-resonance spectra of maize (Zea mays L.) root tips, that had been induced to extrude large amounts of H+ in response to fusicoccin (FC) in the presence of potassium salts, indicate that the cytoplasmic pH does not become higher than that of controls. In fact, the cytoplasmic pH may become slightly (approx. 0.1 pH unit) lower in cells extruding H+. Estimations of the buffer capacity of the cells show that without active intracellular pH regulation, H+ extrusion caused by FC would cause the intracellular pH to rise by at least 0.6 pH unit h-1. Our results indicate that intracellular pH is tightly regulated even during extreme rates of acid extrusion, and that a rise in cytoplasmic pH is not the signal linking H+ extrusion with enhanced organic-acid synthesis or other intracellular responses to H+ pumping.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 20
    ISSN: 1432-2048
    Schlagwort(e): Acid growth ; Geotropism ; Helianthus ; Phototropism ; Proton secretion ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract By placing seedlings of sunflower (Helianthus annuus L.) or maize (Zea mays L.) on agar plates containing a pH indicator dye it is possible to observe surface pH patterns along the growing seedling by observing color changes of the indicator dye. Using this method we find that in geotropically stimulated sunflower hypocotyls or maize coleoptiles there is enhanced proton efflux on the lower surface of the organ prior to the initiation of curvature. As curvature develops the pattern of differential acid efflux becomes more intense. A similar phenomenon is observed when these organs are exposed to unilateral illumination, i.e. enhanced acid efflux occurs on the dark side of the organ prior to the initiation of phototropic curvature and the pattern of differential acid efflux intensifies as phototropic curvature develops. These observations indicate that differential acid efflux occurs in response to tropistic stimuli and that the acid efflux pattern may mediate the development of tropistic curvatures.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 21
    ISSN: 1432-2048
    Schlagwort(e): Auxin uptake ; Coleoptile ; Fluorescein ; Morphactin ; Naphthylphthalamic acid ; Triiodobenzoic acid ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Using both 1-mm segments of corn (Zea mays L.) coleoptiles and a preparation of membranes isolated from the same source, we have compared the effectiveness of several inhibitors of geotropism and polar transport in stimulating uptake of auxin (indole-3-acetic acid, IAA) into the tissue and in competing with N-1-naphthylphthalamic acid (NPA) for a membrane-bound site. Low concentrations of 2,3,5-triiodobenzoic acid (TIBA), NPA, 2-chloro-9-hydroxyfluorene-9-carboxylic acid (morphactin), and fluorescein, eosin, and mercurochrome all stimulated net uptake of [3H]IAA by corn coleoptile tissues while higher concentrations reduced the uptake of both [3H]IAA and another lipophilic weak acid, [14C]benzoic acid. Since low concentrations of fluorescein and its derivatives competed for the same membrane-bound site in vitro as did morphactin and NPA, the basis for both the specific stimulation of auxin accumulation and the inhibition of polar auxin transport by all these compounds may be their ability to interfere with the carrier-mediated efflux of auxin anions from cells. At higher concentrations, the decrease in accumulation of weak acids was nonspecific and thus may be the result of acidification of the cytoplasm and a general decrease in the driving force for uptake of the weak acids. Triiodobenzoic acid was an exception. Low concentration of TIBA (0.1–1 μM) were much less effective than NPA in competing for the NPA receptor in vitro, but little different from NPA in ability to stimulate auxin uptake. One possibility is that TIBA, a substance which is polarly transported, may compete with auxin for the polar transport site while NPA, morphactin, and the fluorescein derivatives may render this site inactive.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 22
    Digitale Medien
    Digitale Medien
    Springer
    Planta 152 (1981), S. 397-407 
    ISSN: 1432-2048
    Schlagwort(e): Cytokinesis ; High voltage electron microscopy ; Mitosis ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The structure and distribution of cytoplasmic membranes during mitosis and cytokinesis in maize root tip meristematic cells was investigated by low and high voltage electron microscopy. The electron opacity of the nuclear envelope and endoplasmic reticulum (ER) was enhanced by staining the tissue in a mixture of zinc iodide and osmium tetroxide. Thin sections show the nuclear envelope to disassemble at prophase and become indistinguishable from the surrounding ER and polar aggregations of ER. In thick sections under the high voltage electron microscope the spindle is seen to be surrounded by a mass of tubular (TER) and cisternal (CER) endoplasmic reticulum derived from both the nuclear envelope and ER, which persists through metaphase and anaphase. At anaphase strands of TER traverse the spindle between the arms of the chromosomes. The octagonal nuclear pore complexes disappear by metaphase, but irregular-shaped pores persist in the membranes during mitosis. It is suggested that these form a template for pore-complex reformation during telophase. Phragmoplast formation is preceded by an aggregation of TER across the spindle at anaphase. Evidence is presented to suggest that the formation of the desmotubule of a plasmodesma is by the squeezing of a strand of endoplasmic reticulum between the vesicles of the cell plate.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 23
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 129 (1981), S. 349-352 
    ISSN: 1432-072X
    Schlagwort(e): Rhizobium ; Bacteroids ; Viability ; Development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Viability of Rhizobium leguminosarum bacteroids isolated from Pisum sativum was determined after sucrose gradient centrifugation of the bacteroids resulting in separation according to developmental stage. The results suggest that past a critical stage of development bacteroids lose viability. Similar experiments with R. japonicum bacteroids having a lower degree of morphological differentiation showed an appreciably higher viability in all gradient fractions. The results support the hypothesis that bacteroid morphology and viability is dependent on the nature of—and length of exposure to—the host plant cytoplasm.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 24
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 39-48 
    ISSN: 1573-1561
    Schlagwort(e): Insect feeding deterrents ; antifeedants ; Pissodes strobi Peck ; Coleoptera ; Curculionidae ; Thuja plicata Donn
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The feeding deterrent activity of fractions from the foliage of western red cedar,Thujaplicata Donn, was studied in laboratory bioassays using the white pine weevil,Pissodes strobi Peck, as a test insect. The most active fraction was the volatile mixture that comprises the leaf oil of this tree species. Further fractionation of the leaf oil indicated feeding deterrent activity in the monoterpene hydrocarbon, thujone, and terpene alcohol fractions. When tested alone, both (−)-3-isothujone and (+)-3-thujone, which made up 75–88% and 5–10% of the leaf oil, respectively, deterred feeding by the weevils. Western red cedar leaf oil also showed antifeedant activity with the alder flea beetle,Altica ambiens (Le Conte), and served as an oviposition deterrent for the onion root maggot,Hylemya antiqua Meigen. The leaf oil, however, had no inhibitory effect on the feeding of the leaf roller,Epinotia solandriana L., and the red-backed sawfly,Eriocampa ovata L.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 25
    Digitale Medien
    Digitale Medien
    Springer
    Planta 153 (1981), S. 447-452 
    ISSN: 1432-2048
    Schlagwort(e): Auxin binding ; Root (auxin) ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Auxin binding onto membrane fractions of primary roots of maize seedlings has been demonstrated using naphth-1yl-acetic acid (NAA) and indol-3yl-acetic acid (IAA) as ligands. This binding is compared with the already well characterized interaction between auxins and coleoptile membranes. The results indicate that while kinetic parameters are of the same order for root and coleoptile binding, a number of differences occur with respect to location in cells and relative affinity. The possible significance of the existence of such binding sites in root cells is discussed in relation to auxin action.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 26
    Digitale Medien
    Digitale Medien
    Springer
    Planta 153 (1981), S. 453-458 
    ISSN: 1432-2048
    Schlagwort(e): Abscisic acid ; Gravireaction ; Growth inhibitors ; Root ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Using gas chromatography-mass spectrometry (GC/MS) techniques of analyses, it has been found that endogenous abscisic acid (ABA) becomes asymmetrically distributed in the elongation zone of horizontal Zea mays (cv. LG 11) roots which are showing a positive gravitropic response. There is a relative increase in the ABA content of the lower half and a concomitant decrease for the upper half in such roots. Asymmetric distribution of ABA is also detected in the elongation zone of half-decapped roots.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 27
    ISSN: 1432-2048
    Schlagwort(e): Aerenchyma ; Ethylene ; Gas space ; Oxygen and gas-space formation ; Roots, adventitious ; Silver ions ; Zea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have studied the role of ethylene in accelerating the lytic formation of gas spaces (aerenchyma) in the cortex of adventitious roots of maize (Zea mays L.) growing in poorly aerated conditions. Such roots had previously been shown to contain increased concentrations of ethylene. Ten day-old maize plants bearing seminal roots and one whorl of emerging adventitious roots were grown in nutrient solution bubbled with air, ethylene in air (0.1 to 5.0 μl l−1), or allowed to become oxygen-deficient in nonaerated (but not completely anaerobic) solution. Additions of 0.1 μl l−1 ethylene or more promoted the formation of aerenchyma, with lysis of up to 47% of the cortical cells. The effects of non-aeration were similar to those of exogenous ethylene. When silver ions, an ethylene antagonist, were present at low, non-toxic concentrations (circa 0.6 μM), aerenchyma formation was prevented in ethylene treated roots and in those exposed to oxygen deficiency. Silver ions also blocked the inhibiting effect of exogenous ethylene on root extension. By contrast, the suppression of aerenchyma formation by silver ions under oxygendeficient conditions was associated with a retardation of root extension, indicating the importance of aerenchyma for root growth in poorly aerated media. Rates of production of ethylene by excised roots were stimulated by a previous non-aeration treatment. The effectiveness of Ag+ in inhibiting equally the action on cortical cells of exogenous ethylene and of non-aeration, supports the view that gas space (aerenchyma) formation in adventitious roots ‘adpted’ to oxygendeficient environments is mediated by increased concentrations of endogenous ethylene. The possibility that extra ethylene could arise from increased biosynthesis of a precursor in root tissues with a restricted oxygen supply is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 28
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 217 (1981), S. 65-77 
    ISSN: 1432-0878
    Schlagwort(e): Freeze-fracture ; Occluding junctions ; Enterocytes ; Development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The architecture of occluding junctions during the differentiation of the mouse duodenum was studied in freeze-fractured material. Irregular zonulae occludentes (ZO) (Type I) are numerous during fetal life, and are characterized by their irregular width, and by the presence of basal open-ended extensions fused with the discontinuous basal strand of the ZO. Regular ZOs (Type II), typical of the adult villous epithelium, appear after Type I junctions by day 16 of gestation. Two patterns are distinguishable: in the first, parallel strands of ridges and furrows are found without crossing branches; in the second pattern, the junction zone is organized like a network of short branches forming various types of polygons. In fetal and adult mice fasciae occludentes (FO) (Type III) are present on the lateral cell membranes; in unfixed specimens particles are found in the furrows of the E-face and pits on the ridges of the P-face. In fixed tissues, the particles are aligned on the ridges of the P-face. These results indicate that fixation with glutaraldehyde modifies considerably the affinity of junctional particles toward the P-face during the fracture process. Moreover, the presence of numerous large FOs on the lateral cell membranes of enterocytes during late fetal life and in the adult, is possibly related to cell movement along the intestinal villi.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 29
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 216 (1981), S. 377-401 
    ISSN: 1432-0878
    Schlagwort(e): Lamina ganglionaris ; Locust ; Neurons ; Development ; Light and electron microscopy ; Golgi study
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The gross structure as well as the neuronal and non-neuronal components of the lamina ganglionaris of the locust Schistocerca gregaria are described on the basis of light- and electron-microscopical preparations of Golgj (selective silver) and ordinary histological preparations. The array of optic cartridges within the lamina neuropile — their order and arrangement — and the composition of the cartridges are described. There are six types of monopolar neurons: three whose branches reach to other cartridges and three whose branches are confined to their own cartridges. Retinula axons terminate either in the lamina or the medulla neuropiles. There are three types of centrifugal neurons, two types of horizontal neuron, as well as glia and trachea in the lamina neuropile. The development of the lamina neuropile is described in terms of developing monopolar and centrifugal axons, growing retinula fibres, and composition of the developing optic cartridges.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 30
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 217 (1981), S. 155-170 
    ISSN: 1432-0878
    Schlagwort(e): Adrenal medulla ; Development ; Acetylcholinesterase ; Small granule cells ; Ultramorphology ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The development of the rat adrenal medulla was studied at the ultrastructural level with particular emphasis placed on early discrimination of different catecholamine-storing cells. The first granule-containing cells, phaeochromoblasts, were seen at day 15 of gestation migrating into the anlage of the cortex. These cells were characterized by a few small granules (80–120 nm in diameter) and a high nuclear to cytoplasmic ratio. Presumably due to differentiation into chromaffin cells, they were no longer present after the eighth postnatal day. Maturation of phaeochromoblasts was indicated by an increase in number and size of their storage granules and a decrease in the nuclear to cytoplasmic ratio. Noradrenaline and adrenaline cell types were first clearly discernible at day 21 of gestation. Another cell type, a giant cell, was also recognized at this stage. In the adult animal, noradrenaline, two morphologically different types of adrenaline, and small granule-containing cells were observed. By applying acetylcholinesterase histochemistry, it was found that at day 17 of gestation a small population of granule-storing cells showed strong positive staining in the endoplasmic reticulum. In the adult animal this cell type was further characterized by small-storage granules. Other chromaffin cells began to show weak staining within the endoplasmic reticulum at day 19 of gestation. This staining appeared more frequently within adrenaline than noradrenaline cells. However, even in the adult animal many cells of both types were completely negative. It is concluded that acetylcholinesterase histochemistry is a useful method for early discrimination of small granule-containing cells in the developing rat adrenal medulla.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 31
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 215 (1981), S. 563-575 
    ISSN: 1432-0878
    Schlagwort(e): Antennal lobes ; Development ; Sexual dimorphism ; Periplaneta americana
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The postembryonic development of the antennal lobes of Periplaneta americana L. was examined with light- and electron-microscopical methods. There is no difference in the number of glomeruli and neurons in the antennal lobes of larval and adult animals. At hatching, the first larva already possesses the adult number of approximately 125 glomeruli and 500 to 560 deutocerebral neurons in the dorsolateral cell group of each antennal lobe. During postembryonic development the volume of the deutocerebral neurons increases three- to fourfold. The glomeruli of the first larva have about 7 % of the volume of the corresponding adult glomeruli. Since number, pattern, and size ratio of glomeruli (with the exception of the macroglomerulus) are constant in all larval stages and adult animals, it is possible to identify individual glomeruli. During the whole postembryonic development the ordinary glomeruli show a continuous volume increase, which parallels the increase in antennal sensory input. The macroglomerulus develops by way of special growth of two to four neuropil units, but not before the last three to four larval stages and only in males. Its growth precedes the formation of antennal pheromone receptors during the final molt; these receptors are known to project into the macroglomerulus. The development of the macroglomerulus in the last larval stages of the male may be caused by a genetically fixed growth program of specific deutocerebral neurons.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 32
    Digitale Medien
    Digitale Medien
    Springer
    Protoplasma 108 (1981), S. 83-98 
    ISSN: 1615-6102
    Schlagwort(e): Development ; Etioplast ; Prolamellar body ; Protochlorophyll ; Saponin ; Thylakoid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Etioplasts of dark grown plants contain a large paracrystalline prolamellar body (PLB) and, attached to this there are prothylakoid membranes (PTs). PLB-tubules inAvena are composed mainly of two saponins and include only a low percentage of other lipids, protochlorophyll(ide) and proteins. Following the development of etioplasts in darkness from the very beginning until plants loose turgescence one can observe marked changes in ultrastructure. In the early stage of development predominantly PTs are seen in small etioplasts. Wide-type PLBs are small. After eight days there is a well developed stage with the well-known big and highly crystalline PLBs, which are connected to many long PT-membranes. After 13 days the PLBs are not significantly changed, while number and length of PTs are strongly reduced. These morphological observations are quantified by measurements of PLB-area and PT-length per plastid section. Saponin content as a marker for PLB-tubules and protochlorophyll(ide)-content as a marker for PT-membranes were measured. Both methods of determination show in good agreement a peak of development for PTs around day 6–7, and for PLBs around 9–10. Beginning senescence affects PT-membranes and PChl(ide) strongly, while saponins resp. PLBs persist better. These results are presented in view of thylakoid formation during greening, starting from the different etioplast stages.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 33
    ISSN: 1615-6102
    Schlagwort(e): Calcium-oxalate crystals ; Development ; Mucilage ; Opuntia ficus-indica ; Stereology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In the succulent tissue ofOpuntia ficusindica development of calcium oxalate crystals was followed from the shoot apex to its base, using stereological analysis. The stereological parameters relating to the crystal cells were compared with those of mucilage cells, of the same tissue. A fairly monotonic relation between the stereological parameters of the two populations was noted. The possible physiological relation between the two cell populations is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 34
    Digitale Medien
    Digitale Medien
    Springer
    Behavior genetics 11 (1981), S. 145-151 
    ISSN: 1573-3297
    Schlagwort(e): Bruchidae ; Callosobruchus ; Coleoptera ; nonadditive inheritance ; oviposition behavior
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Psychologie
    Notizen: Abstract As a result of different feeding regimens, two laboratory populations of the beetleCallosobruchus maculatus Fab. developed different rates of oviposition. The behavior of the F1 hybrids cannot be explained with an additive model of inheritance. The unusual pattern of inheritance suggests a sex-related factor(s) and a two-factor interaction.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 35
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 1-7 
    ISSN: 1573-1561
    Schlagwort(e): Japanese beetle ; Popillia japonica ; Coleoptera ; Scarabaeidae ; sex attractant ; survey lure ; phenethyl propionate ; eugenol ; synergism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract A combination of the synthetic sex attractant (R,Z)-5-(1-decenyl) dihydro-2(3H)-furanone with a 3∶7 mixture of phenethyl propionate (PEP) and eugenol (4-allyl-2-methoxphenol) caught significantly morePopillia japonica Newman than either the sex attractant or the mixture did alone. Also, the synthetic sex attractant captured significantly more males than the PEP-eugenol did during the period of heavy adult emergence of the beetles. The two lures were not significantly different in their attractancy to males about a week later and thereafter. A combination of PEP-eugenol and virgin females in the same trap late in the season also significantly increased beetle captures.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 36
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 9-18 
    ISSN: 1573-1561
    Schlagwort(e): Dendroctonus brevicomis ; Ips paraconfusus ; Coleoptera ; Scolytidae ; Pinus ponderosa ; bark beetle ; exo-brevicomin ; frontalin ; myrcene ; verbenone ; ipsenol ; ipsdienol ; cis-verbenol ; attractants ; inhibition ; semiochemicals ; pheromones
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Dendroctonus brevicomis was attracted to a mixture of theIps paraconfusus pheromones, ipsenol,cis-verbenol, and ipsdienol at 10−9 g each/μl but was not attracted to these pheromones at higher and lower release rates.I. paraconfusus was not attracted to theD. brevicomis pheromonesexo-brevicomin, frontalin, and myrcene at any release rate tested. Increased release rates of a mixture of the three pheromones ofI. paraconfusus inhibited the attraction ofD. brevicomis to its synthetic pheromones. A mixture of ipsenol + ipsdienol orcis-verbenol alone failed to cause inhibition indicating that at least two of theI. paraconfusus pheromones are required to inhibit the response ofD. brevicomis. The pheromones ofD. brevicomis did not inhibit the attraction ofI. paraconfusus to its pheromones; however, verbenone was a potent inhibitor.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 37
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 33-38 
    ISSN: 1573-1561
    Schlagwort(e): Coleoptera ; Bostrichidae ; Rhyzopertha dominica ; aggregation pheromone ; lesser grain borer ; grain insect pest
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Adult males of the lesser grain borer,Rhyzopertha dominica (F.), produce a pheromone that attracts both sexes. The volatiles from males collected on filter paper or Porapak-Q were attractive in two laboratory bioassays. The mating behavior is described and the function of the phen infested with the borer.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 38
    ISSN: 1573-1561
    Schlagwort(e): Primary attraction ; tree predisposition ; Dendroctonus ; Ips ; Gnathotrichus ; Pityophthorus ; Coleoptera ; Scolytidae ; Buprestidae ; Verticicladiella ; Pinus ponderosa
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Detection of weakened hosts from a distance by bark beetles through olfaction was investigated in field experiments. No significant numbers of Scolytidae were attracted to anaerobically treated pine bolts, stem disks, or sugar and ponderosa pine bark including phloem. Treatment of living trees with cacodylic acid induced attacks byDendroctonus brevicomis, D. ponderosae, Ips latidens, Gnathotrichus retusus, andPityophthorus scalptor, beginning two weeks after treatment. There was no significant difference between landing rates ofD. brevicomis andD. ponderosae on screened treated trees and screened controls. There was a significant increase in landing rates ofG. retusus andI. latidens, because both species had penetrated the screen and produced pheromones. Tree frilling alone did not increase the landing rate of bark beetles. Freezing of the lower trunk with dry ice did not increase significantly the landing rate ofD. brevicomis, D. ponderosae, G. retusus, orI. latidens on screened trees, whereas unscreened frozen trees were attacked by all four species. There was no significantly higher landing rate byD. brevicomis, D. ponderosae, I. paraconfusus, I. latidens, G. retusus, orHylurgops subcostulatus on screened trees evidencing symptoms of severe infection by the root pathogenVerticicladiella wagenerii, than on symptornless trees. These experiments show thatD. brevicomis, D. ponderosae, I. paraconfusus, I. latidens, andG. retusus land, apparently indiscriminately, on healthy and stressed hosts. Thus, in these species host discrimination must occur after landing and prior to sustained feeding.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 39
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 359-366 
    ISSN: 1573-1561
    Schlagwort(e): Parasites ; bioassay ; Dendroctonus frontalis ; Coleoptera ; Scolytidae ; olfactometer ; bark beetles
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract An “H”-type olfactometer was designed and built to test the response of bark beetle parasites to various beetle and tree host odors. The design has several advantages over other types of olfactometers. Strong air currents are not utilized, parasites have free movement in the test chamber, and a concentration gradient of test compound is maintained. Parasites tested in the olfactometer demonstrated strong positive responses to air drawn over logs infested with bark beetle larvae and varied responses to tree host odors.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 40
    ISSN: 1573-1561
    Schlagwort(e): Sexual dimorphism ; Tribolium castaneum (Herbst) ; Coleoptera ; Tenebrionidae ; aggregation pheromone ; behavior ; bioassay ; red flour beetle
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Evidence for the existence of a male-produced aggregation pheromone secreted from the prothoracic femoral setiferous sex patch ofTribolium castaneum is reported. Both sexes were attracted toca. 60 ng of crude secretion. Males and females perceive the pheromone on the day of emergence while perception differs between the sexes: male response reaches a maximum on day 1 posteclosion, when tested at 〈1, 1, and 30 days; females show a maximum response at 30 days posteclosion. Behavioral responses to pheromone odors and a complex Chromatographic profile are reported.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 41
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 889-894 
    ISSN: 1573-1561
    Schlagwort(e): α-Pinene ; defensive secretions ; interspecific variation ; limonene ; monoterpenes ; pentadecene ; Artystona sp. ; Coleoptera ; Tenebrionidae ; lichen ; Parmotrema reticulatum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The defensive secretions of four species of the genusArtystona endemic to New Zealand differ from those of other tenebrionids in that they contain α-pinene and limonene, as well as the more characteristic quinones and alkenes. Adults and larvae ofA. obscura, A. erichsoni, A. rugiceps, andArtystona sp. feed on the lichenParmotrema reticulatum (Taylor), but the terpenes are not sequestered from it. The defensive secretions of the four species show some interspecific variation.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 42
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 1135-1147 
    ISSN: 1573-1561
    Schlagwort(e): Ips paraconfusus ; Coleoptera ; Scolytidae ; Pinus ponderosa ; pheromone biosynthesis ; bark beetle ; myrcene ; α-pinene ; ipsenol ; ipsdienol ; cis-verbenol ; myrtenol ; attractants ; pheromones
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Attraction of maleIps paraconfusus to male-infested ponderosa pine logs was inhibited by volatiles from logs infested with mated males and females over an 8-day period in the field. The response of females during this time was not significantly inhibited by these volatiles. Synthesis of the male-specific pheromones, ipsenol and ipsdienol, appeared negligible after 8 days in males allowed to mate with 3 females in these logs while males alone contained levels of these pheromones at about half their maximum rate. The decline in pheromone production in mated males appears to result, at least in part, from a reduction in the activity of the biosynthetic system that converts the host monoterpene, myrcene, to ipsenol and ipsdienol. Mating and feeding have apparently no effect on the biosynthetic system that converts the host monoterpene, (−)-α-pinene, to the pheromone,cis-verbenol in either males or females. The reduced production and release of pheromones by males after mating appears to play a major function in the process of terminating the aggregation phase of host colonization.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 43
    ISSN: 1573-1561
    Schlagwort(e): Dihydromatricaria acid ; antifeedant ; acetylenic acid ; Chauliognathus pennsylvanicus ; Coleoptera ; Cantharidae ; soldier beetles ; Phidippus ; jumping spider ; bioassay ; defensive secretion ; defensive behavior
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The acetylenic acid,Z-dihydromatricaria acid (DHMA), previously isolated from the defensive secretion ofChauliognathus lecontei, and now shown to occur also inC. pennsylvanicus, is a potent feeding deterrent to jumping spiders (Phidippus spp.). A simple bioassay withPhidippus is described, which is generally applicable to studies dealing with the isolation and evaluation of feeding deterrency of natural products from insects. By use of this assay,Phidippus were shown to be sensitive to as little as 1 μg DHMA, an amount equivalent to less than 2% of the DHMA content ofC. pennsylvanicus.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 44
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 175-182 
    ISSN: 1573-1561
    Schlagwort(e): Hydrocarbons ; n-alkanes ; branched-chain alkanes ; GC-MS ; cuticular wax ; insect ; cowpea weevil ; Callosobruchus maculatus ; Coleoptera ; Curculionidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The composition of the cuticular hydrocarbons of the cowpea weevil,Callosobruchus maculatus (F.), was determined by using combined gas chromatography-mass spectrometry. The hydrocarbons constituted 88% of the cuticular lipids and were composed of four homologous series of alkanes. Mono- and dimethyl branched-chain alkanes made up 83% of the hydrocarbon fraction.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 45
    ISSN: 1573-1561
    Schlagwort(e): Pheromone ; Rhyzopertha dominica ; lesser grain borer ; Coleoptera ; Bostrichidae ; unsaturated ester ; dominicalure ; 1-methylbutyl (E)-2-methyl-2-pentenoate ; 1-methylbutyl (E)-2,4-dimethyl-2-pentenoate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Volatiles from lesser grain borers,Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), were collected on Porapak Q, and those from the male beetles were shown to contain two compounds, that were attractive individually and in combination to both sexes. These compounds were identified as (S)-(+)-1-methylbutyl (E)-2-methyl-2-pentenoate and (S)-(+)-1-methylbutyl (E)-2,4-dimethyl-2-pentenoate by spectrometry and comparison with synthesized compounds. The two compounds have been assigned the trivial names dominicalure 1 and dominicalure 2, respectively. Synthesized samples of these compounds, individually and in combination, were effective in trapping both sexes in field studies.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 46
    ISSN: 1573-1561
    Schlagwort(e): electroantennogram ; boll weevil ; grandlure ; pheromone ; Anthonomus grandis Boh. ; Coleoptera ; Curculionidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Electroantennogram techniques were used to elucidate antennal olfactory response of male and female boll weevils to a dilution series of grandlure, its components, and some vicinal dimethyl analogs. At higher concentrations, response to the mixture of the two aldehyde components of grandlure was significantly higher than to the two alcohol components. Only one vicinal dimethyl analog elicited a significantly higher response than the control. There were no significant differences in response due to sex over all compounds.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 47
    ISSN: 1573-1561
    Schlagwort(e): Tramiathaea cornigera ; Thamiaraea fuscicornis ; Aleocharinae ; Coleoptera ; Staphylinidae ; defensive secretion ; ethyl decanoate ; esters ; undecane ; toluquinone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The defensive secretions of bothTramiathaea cornigera andThamiaraea fuscicornis contain undecane, toluquinone, ethyl decanoate and smaller amounts of other esters. The presence of esters particularly ethyl decanoate as a major component sets these two beetles apart chemically not only among the staphylinids but also within the subfamily Aleocharinae.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 48
    ISSN: 1573-1561
    Schlagwort(e): Pinus ponderosa ; Dendroctonus brevicomis ; western pine beetle ; attractant ; interruption ; behavior ; pheromone ; Coleoptera ; Scolytidae ; exo-brevicomin ; frontalin ; myrcene ; verbenone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The catch of the western pine beetle,Dendroctonus brevicomis, at an attractive source of racemicexo-brevicomin, racemic frontalin, and myrcene was reduced by surrounding the source with a grid of 48 stations releasing all three compounds together, orexo-brevicomin alone or myrcene alone. Each compound was released at the rate of 2 mg/24 hr/station. The catch at an attractive bolt cut from a tree being colonized byD. brevicomis was not reduced byexo-brevicomin, but was reduced by the combination ofexo-brevicomin, frontalin, and myrcene in one of two tests. When a transect of traps was placed across a 0.81-hectare plot at six of the 48 stations releasing all three compounds, more beetles were caught at outer than at inner traps. More beetles were caught at unbaited traps on trees in a plot when the three compounds were released than when onlyexo-brevicomin or no compounds were released. A few trees were attacked byD. brevicomis in some of the plots. The antiattractant verbenone released from 48 stations at the rate of 4 mg/24 hr/station did not reduce the catch at an attractive tree bolt.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 49
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 7 (1981), S. 209-220 
    ISSN: 1573-1561
    Schlagwort(e): Ips paraconfusus ; Coleoptera ; Scolytidae ; phloem ; ponderosa pine ; host selection ; feeding stimulants ; extracts ; bark beetle
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract MaleIps paraconfusus Lanier bored and fed in cellulose powder substrates treated with solvent extracts of ponderosa pine phloem in preference to cellulose powder alone. Stimuli that elicit boring and feeding behavior occurred in the water extracts and the combined solvent extracts. No significant boring or feeding occurred in the methanol extract. There was a preference for, but no significant feeding in, the water partition of the ether extract. Feeding, but no preferential boring, occurred in the ether extracts.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 50
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 215 (1981), S. 171-179 
    ISSN: 1432-0878
    Schlagwort(e): Polychaeta, Serpulidae ; Gamete ; Larva ; Development ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Gametes and developing larvae of the polychaete Galeolaria caespitosa were examined by scanning electron microscopy. The sperm display a primitive morphology. When treated with 0.33 M CaCl2, they release a branched acrosomal process. At spawning, the polygonal oocytes have a granular surface made up of spherules and the tips of microvilli. The oocyte coat develops a ridged appearance as the oocyte rounds up. At fertilization, the microvilli are withdrawn from the coat surface. Microvilli again appear on the coat surface during the trochophore stage, but the egg coat appears to be retained as the larval cuticle until the demersal stage. The surface of the larva now shows many microvilli. Details of the organization of several ciliary structures are clarified. Moreover, the present study shows rapid, sequential development of paired setal sacs, with the most anterior pair appearing first.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 51
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 215 (1981), S. 397-415 
    ISSN: 1432-0878
    Schlagwort(e): Stomach ; Epithelium ; Teleost ; Development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The development of the stomach of the teleost, Clarias lazera, during the early posthatching period, is described, and the developing stomach is compared with that of adult Clarias. The stomach develops in two distinct parts: the corpus, which differentiates first, and the pylorus. The corpus contains a mucous surface epithelium, arranged in folds, and a tubular gland system containing only one type of gland cell, to which the secretion of pepsinogen and HCl is attributed. The pyloric region does not contain tubular glands. From the ultrastructure of the gland cells, the 3H-thymidine labeling index, and the onset of acid production (as determined with pH indicators) it is concluded that a functional stomach is present in juveniles with a standard length of ± 11 mm (approximately 12 days after fertilization at 23–24° C). The ultrastructure of the intestinal epithelium has also been studied. The intestine consists of three segments, similar to those described for stomachless teleosts and a number of fish larvae. In larvae as well as in juveniles, the enterocytes of the second segment show pinocytosis of horseradish peroxidase, although in the juveniles the stomach has already developed. This second segment has the same relative length in all studied larvae and juveniles and is also present in adult Clarias. It is therefore concluded that the capacity to absorb protein macromolecules is not specifically related to the absence of a functional stomach in this teleost species.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 52
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 219 (1981), S. 297-311 
    ISSN: 1432-0878
    Schlagwort(e): Choroid plexus (chick) ; Third ventricle ; Ependyma ; Development ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The surface morphology of the diencephalic choroid plexus (Pl. ch. v. III) was investigated by light microscopy and scanning electron microscopy in chicks from the 7th embryonic day (ED) to the 8th week after hatching. Pl. ch. v. III develops on the anterior ventricular roof from a sagittally oriented fold and a few posteriorly located transverse folds. On the 7th ED no significant differences in the cell surface morphology between Pl. ch. v. III and the surrounding ependyma are observed: both are covered with cilia. During the next four days, long cell prolongations (one per cell) covered with microvilli develop first on the surface of the posterior ventricular roof and then on the posterior part of Pl. ch. v. III. These structures are transitory. On the 11th ED, round cell prolongations (one per cell) appear progressively on the entire plexus, also replacing the long ones. Now the plexus surface is distinct from the surface of the surrounding ependyma. During the last week before hatching and also after hatching, the round cell prolongations become less prominent. Simultaneously, the number of cilia per unit surface area diminishes. With consideration of earlier reports, this study suggests that the following factors are involved in the increase of the surface area of Pl. ch. v. III: (I) The pseudostratified epithelium changes into columnar epithelium. (2) Ependymal elements of the posterior roof of the 3rd ventricle contribute to the anlage of Pl. ch. v. III. In later stages, however, Pl. ch. v. III grows only by mitoses.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 53
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 220 (1981), S. 337-347 
    ISSN: 1432-0878
    Schlagwort(e): Pineal ; Monolayer culture ; Development ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The morphological development of pinealocytes maintained in monolayer culture, without the neural and humoral effects present in the developing rat has been studied and compared with the development that occurs in vivo. Pinealocytes in 5 day cultures contained organelles that were similar to those present in the pineals of intact 5 day old rats. However, light and dark cells were not noted in culture, and the cultured cells did not have the dense granules noted in vivo. As pinealocytes developed in culture, cytoplasmic processes increased in length and number. By 21 days of culture age, synaptic ribbons were found to have decreased in number, the difference between light cell and dark cell cytoplasm had become more prominent, and dense-cored vesicles had become more numerous, just as in the developing gland in vivo. These results suggest that the complex neural and humoral factors impinging upon the developing neonatal pineal in the intact animal may not be necessary for some aspects of its ultrastructural differentiation.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 54
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 214 (1981), S. 271-278 
    ISSN: 1432-0878
    Schlagwort(e): Blood-CSF barrier ; Blood-brain barrier ; Horseradish peroxidase ; Choroid plexus ; Development ; Chick embryo
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The penetration of horseradish peroxidase (HRP) from blood into ventricle via the avian choroid plexus was examined by electron microscopy in order to study the development of the blood-cerebrospinal fluid barrier (BCSFB). 1–20 mg HRP was injected into the allantoic vein of chick embryos between the 7th and 21st day of incubation. Until the 8th day (stage 34) the reaction products of injected HRP were observed in the interepithelial clefts at both luminal (ventricular) and abluminal sides. At the 9th day (stage 35), their penetration was blocked at most apical junctional complexes of the choroidal epithelia. At the 10th day (stage 36) and at every subsequent stage, HRP molecules were completely impeded at the apical tight junctions. These results show that the BCSFB to HRP in the avian choroidal epithelium is completely established by the 10th day, when molecules of HRP are still able to permeate between the capillary endothelia in the cerebellum and the spinal cord (Wakai and Hirokawa 1978).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 55
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 218 (1981), S. 41-58 
    ISSN: 1432-0878
    Schlagwort(e): Supraependymal cell clusters ; Ventricular system ; Development ; Hypothalamus rat ; Monosodium glutamate ; Androgenized rats
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The histological patterns of supraependymal cell clusters (CC) in rats of different ages (untreated, androgenized, and treated with monosodium glutamate) were investigated with light (LM)-, scanning-and transmission electron microscopy (SEM, TEM). These clusters were a frequent but not a constant finding. In 18 day-and older embryos, CC were always found in the recess of the olfactory bulb immediately prior to its obliteration. All other CC appear in the infundibular recess between the 3rd and the 6th postnatal day. Independent of age, all cell clusters exhibit small aggregates of subependymal tissue protruding through the ependyma. Both neurons (light cells) and neuroglia (dark cells) were found in the CC. By use of SEM, in the region of the infundibular recess it is possible to distinguish four forms of supraependymal cell clusters according to localization, size, number of cells, and presence of intraventricular axons. CC may be 1) receptors or have an additional secretory function; 2) manifestations of a pathological type of reaction of the ventricular wall; 3) possible excrescences of the neural matrix, or 4) modifications of the ventricular wall in relation to the obliteration of the ventricular recesses. The first two interpretations are not tenable based on the present observations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 56
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 221 (1981), S. 431-442 
    ISSN: 1432-0878
    Schlagwort(e): Kidney (rabbit) ; Development ; Mesonephric nephron ; Freeze-fracture electron microscopy ; Intercellular junctions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In freeze-fracture replicas of the entire cross-fractured mesonephros of 18 day rabbit embryos the basolateral and luminal cell faces of the different nephron segments were studied and compared with their metanephric counterparts. In the proximal tubule, the shallow zonula occludens exhibited only 1–2 strands and resembled the corresponding metanephric zonula, a very “leaky” type, which was found with a considerable paracellular flow component in sites of isotonic reabsorption. Gap junctions were restricted to the proximal tubule and were seen more frequently in its terminal segment. The distal tubule harboured two types of tight junctions. The most common type, a band of 5–8 closely parallel strands, matched the zonula occludens of the metanephric straight distal tubule. The observed particle density of the basolateral membrane (2,500±328/μm2) was less than that of the proximal tubule (2,642±306). In addition, the collecting tubule exhibited a zonula occludens of the “tight” variety similar to that which occurred in the metanephric collecting duct. Rod-shaped particles of the luminal membrane were mainly concentrated in some of the intercalated cells but also had developed on principal cells, and occasionally, in the distal tubule. The Wolffian duct, with a deep “tight” zonula occludens, had an obviously rather inactive epithelium with no conspicuous transport-linked membrane specializations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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