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  • Nitrogen fixation
  • stress
  • Springer  (73)
  • Springer Science + Business Media
  • 1980-1984  (73)
  • 1925-1929
  • 1983  (51)
  • 1980  (22)
Collection
Publisher
  • Springer  (73)
  • Springer Science + Business Media
Years
  • 1980-1984  (73)
  • 1925-1929
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    International journal of earth sciences 72 (1983), S. 1167-1196 
    ISSN: 1437-3262
    Keywords: Experiment ; anisotropy ; stress ; strain ; pure shear ; simple shear ; buckling ; bending ; macroscopic fabric ; symmetry ; flexural-slip fold ; kink band ; Jura-Mountains ; Rhenish Massif (Rheinisches Schiefergebirge, Ruhr district)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Description / Table of Contents: Abstract An applicable interpretation of fabrics should be based mainly on geometrical considerations in order to cover available field data. Therefore a theory on the formation of foldtype fabrics including congruent and concentric flexural-slip folds as well as kink bands in materials subjected to rhombic and different monocline strain types is derived by means of particle motion fields for homogeneous and isotropic bodies. The analysis of experimental results and their comparison with field observations largely confirms the theory and contributes to its improvement. Some trends can be established: With increasing monocline character of the strain type, the probability of concentric and congruent flexural-slip folding is reduced. It is substituted by kink band formation. While the portion of monocline strain is enlarged with depth, rhombic fold symmetry indicates, in the realm of elastic-plastic behaviour, the proximity of the surface of the earth or of a detachment surface. With gradual increase of the rock anisotropy, the development of shear faults, kink folds and, finally, congruent and concentric flexural-slip folds is favoured.
    Abstract: Résumé L'interprétation, en vue de son application, de la genèse des formes structuraux résultant de processus tectoniques doit être basée essentiellement sur des raisonnements géométriques pour qu'elle corresponde à la structure des observations faites sur le terrain. Pour cette raison, une théorie de la formation des formes structuraux d'aspect plissé, y compris des plis semblables ou isopaques à glissement banc sur banc ainsi que des knicks, est développée à partir d'une figuration générale des mouvements des particules dans les corps homogènes et isotropes soumis à des déformations à symétrie orthorhombique ou monoclinique. L'analyse des résultats expérimentaux et des comparaisons avec des observations sur le terrain confirment largement la théorie et contribuent à son perfectionnement. Quelques tendances se dessinent: avec l'accroissement du caractère monoclinique de la déformation s'accroît aussi la probabilité de la formation des knicks plutôt que des plis à glissements banc sur banc, concentriques et congruents. Tandis que la déformation monoclinique prend une part plus importante avec la profondeur, la symétrie orthorhombique des plis en régime de déformation élastoplastique indique la proximité de la surface de la Terre ou d'une surface de décollement. Avec l'augmentation de l'anisotropie des roches, la formation de cassures de cisaillement, de knicks et enfin de plis semblables et isopaques à glissement banc sur banc est favorisée.
    Notes: Zusammenfassung Eine anwendungsbezogene Deutung der Gefügegenese muß in erster Linie auf geometrischen Überlegungen beruhen, damit sie auf Geländedaten übertragen werden kann. Ausgehend von Partikelbewegungsfeldern, die für homogene und isotrope Körper gelten, wird eine Theorie der Genese faltenförmiger Gefüge entwickelt, die konzentrische und kongruente Biegegleitfalten und Knickzonen bei rhombischen und monoklinen Deformationstypen erfaßt. Die Ergebnisse von Experimenten und der Vergleich mit Geländebefunden bestätigen weitgehend die Theorie und tragen zu ihrer Weiterentwicklung bei. Einige Trends zeichnen sich ab: Mit steigender Monoklinität des Deformationstyps nimmt die Wahrscheinlichkeit der Bildung von Knickzonen auf Kosten von konzentrischen und kongruenten Biegegleitfalten zu. Während der Anteil monokliner Deformation zur Tiefe hin größer wird, zeigt eine rhombische Symmetrie der Falten im elastisch-plastischen Bereich die Nähe der Erdoberfläche oder einer Abscherungsfläche an. Bei zunehmender Anisotropie werden zunächst bevorzugt Scherbrüche, dann Knickzonen und schließlich kongruente und konzentrische Biegegleitfalten gebildet.
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  • 2
    Electronic Resource
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    International journal of earth sciences 72 (1983), S. 421-450 
    ISSN: 1437-3262
    Keywords: Experiment ; anisotropy ; stress ; strain ; pure shear ; simple shear ; buckling ; bending ; macroscopic fabric ; symmetry ; flexural-slip fold ; kink band ; Jura-Mountains ; Rhenish Massif (Rheinisches Schiefergebirge, Ruhr district)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Description / Table of Contents: Abstract An applicable interpretation of fabrics should be based mainly on geometrical considerations in order to cover available field data. Therefore a theory on the formation of foldtype fabrics including congruent and concentric flexural-slip folds as well as kink bands in materials subjected to rhombic and different monocline strain types is derived by means of particle motion fields for homogeneous and isotropic bodies. The analysis of experimental results and their comparison with field observations largely confirms the theory and contributes to its improvement. Some trends can be established: With increasing monocline character of the strain type, the probability of concentric and congruent flexural-slip folding is reduced. It is substituted by kink band formation. While the portion of monocline strain is enlarged with depth, rhombic fold symmetry indicates, in the realm of elastic-plastic behaviour, the proximity of the surface of the earth or of a detachment surface. With gradual increase of the rock anisotropy, the development of shear faults, kink folds and, finally, congruent and concentric flexural-slip folds is favoured.
    Abstract: Résumé L'interprétation, en vue de son application, de la genèse des formes structuraux résultant de processus tectoniques doit être basée essentiellement sur des raisonnements géométriques pour qu'elle corresponde à la structure des observations faites sur le terrain. Pour cette raison, une théorie de la formation des formes structuraux d'aspect plissé, y compris des plis semblables ou isopaques à glissement banc sur banc ainsi que des knicks, est développée à partir d'une figuration générale des mouvements des particules dans les corps homogènes et isotropes soumis à des déformations à symétrie orthorhombique ou monoclinique. L'analyse des résultats expérimentaux et des comparaisons avec des observations sur le terrain confirment largement la théorie et contribuent à son perfectionnement. Quelques tendances se dessinent: avec l'accroissement du caractère monoclinique de la déformation s'accroît aussi la probabilité de la formation des knicks plutôt que des plis à glissements banc sur banc, concentriques et congruents. Tandis que la déformation monoclinique prend une part plus importante avec la profondeur, la symétrie orthorhombique des plis en régime de déformation élastoplastique indique la proximité de la surface de la Terre ou d'une surface de décollement. Avec l'augmentation de l'anisotropie des roches, la formation de cassures de cisaillement, de knicks et enfin de plis semblables et isopaques à glissement banc sur banc est favorisée.
    Notes: Zusammenfassung Eine anwendungsbezogene Deutung der Gefügegenese muß in erster Linie auf geometrischen Überlegungen beruhen, damit sie auf Geländedaten übertragen werden kann. Ausgehend von Partikelbewegungsfeldern, die für homogene und isotrope Körper gelten, wird eine Theorie der Genese faltenförmiger Gefüge entwickelt, die konzentrische und kongruente Biegegleitfalten und Knickzonen bei rhombischen und monoklinen Deformationstypen erfaßt. Die Ergebnisse von Experimenten und der Vergleich mit Geländebefunden bestätigen weitgehend die Theorie und tragen zu ihrer Weiterentwicklung bei. Einige Trends zeichnen sich ab: Mit steigender Monoklinität des Deformationstyps nimmt die Wahrscheinlichkeit der Bildung von Knickzonen auf Kosten von konzentrischen und kongruenten Biegegleitfalten zu. Während der Anteil monokliner Deformation zur Tiefe hin größer wird, zeigt eine rhombische Symmetrie der Falten im elastisch-plastischen Bereich die Nähe der Erdoberfläche oder einer Abscherungsfläche an. Bei zunehmender Anisotropie werden zunächst bevorzugt Scherbrüche, dann Knickzonen und schließlich kongruente und konzentrische Biegegleitfalten gebildet.
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  • 3
    ISSN: 1432-2048
    Keywords: Allantoin ; Amino acids ; Bleeding sap ; Nitrogen fixation ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dwarf french beans, Phaseolus vulgaris L., were grown with or without inoculation with rhizobia (strain 3644), and with or without a combined nitrogen source (nitrate or ammonium ions). The distribution of radioactivity into products of dark 14CO2 assimilation was studied in roots or nodules from these plants. A detailed study was also made of the distribution and rates of excretion of nitrogen in xylem bleeding sap in 28 day old plants grown on the various sources of nitrogen. Whereas detached nodules accumulated radioactive glycine, serine and glutamate when incubated with 14CO2, bleeding sap from plants root fed 14CO2 contained low levels of radioactivity in these compounds but higher levels in allantoin. Chemical analysis showed allantoin to be the major compound transported in the xylem of nodulated plants, whether or not they were fed on combined nitrogen. In contrast uninoculated plants accumulated mainly amino acids in the bleeding sap, the amount and chemical composition of which depended on the combined nitrogen source.
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  • 4
    ISSN: 1432-2048
    Keywords: Glyoxylate ; Isonicotinic acid hydracide ; Medicago ; Nitrogen fixation ; Photorespiration ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nitrogen fixation (measured as acetylene reduction) by whole nodulated alfalfa plants was stimulated when the plants were treated with isonicotinic acid hydracide (INH) and glyoxylate, both inhibitors of the glycolate pathway of carbohydrate metabolism, at concentrations of 300 and 100 mM, respectively. Reducing energetic loses caused by photorespiration results in an increase in the symbiotic nitrogen fixation.
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  • 5
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    Environmental management 7 (1983), S. 177-187 
    ISSN: 1432-1009
    Keywords: Nitrogen ; Model ; Agriculture ; Mass balance ; Ground-water ; Denitrification ; Immobilization ; Dry deposition ; Nitrogen fixation ; Nitrate ; Florida
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A detailed nitrogen budget was devised for agricultural activities in the Florida peninsula, based on routine data published by state agricultural agencies. The model demonstrates that important unmonitored fluxes of nitrogen can often be calculated by mass balance on individual model compartments, and that the reasonability of poorly quantified fluxes can be assessed. The results of such models can be very useful in designing and assessing the results of field experiments and in prioritizing environmental monitoring programs.
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  • 6
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    Archives of microbiology 136 (1983), S. 20-25 
    ISSN: 1432-072X
    Keywords: Hydrogen production ; Nitrogen fixation ; Hydrogen recycling ; Hydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mutants of Rhodopseudomonas capsulata lacking uptake hydrogenase activity have been isolated among those unable to grow photoautotrophically. Studies with these mutants showed increases in nitrogenase mediated H2 production from all substrates tested. In addition, photosynthetic synthetic growth on N2 with malate as carbon source was not affeced by the block in H2 uptake even under low light. Under these growth conditions hydrogen was observed to accumulate in mutant but not in wild-type cultures. This finding suggested that H2 was evolved by nitrogenase during N2 fixation by this photosynthetic bacterium and was efficiently recycled in the wild type.
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  • 7
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    Archives of microbiology 135 (1983), S. 287-292 
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Respiration ; Nitrogen fixation ; Heterocysts ; K m for O2 ; Anabaena variabilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Simultaneous measurements of acetylene reduction by Anabaena variabilis and the concentration of dissolved oxygen in the suspension were made using a specially designed vessel which allowed measurements under steady-state conditions. The rate of acetylene reduction in the dark increased with increasing oxygen concentrations until a maximum value was reached at 300 μM O2 (corresponding to 30% O2 in the gas phase at 35°C). This presumably results from a requirement for energy provided by respiration. Measurements of the dependence of respiration rate on dissolved oxygen concentration were made under comparable conditions using an open system to allow conditions close to steady-state to be obtained. The respiration rate of diazotrophically grown Anabaena variabilis had a dependence on oxygen concentration corresponding to the sum of two activities. These had K m values of 1.0 μM and 69 μM and values of V max of similar magnitude. Only the high affinity activity was observed in nitrate-grown cyanobacteria lacking heterocysts, and this presumably represent activity in the vegetative cells. The oxygen concentration dependence of the low affinity activity resembled that for the stimulation of acetylene reduction. We interpret this as the result of oxygen uptake by the heterocysts. The results are consistent with the idea that in intact filaments of cyanobacteria O2 enters heterocysts much more slowly than it enters the vegetative cells.
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  • 8
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    Archives of microbiology 127 (1980), S. 163-165 
    ISSN: 1432-072X
    Keywords: Nitrogen fixation ; Arthrobacter ; Corynebacterium ; Anabaena azollae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Coryneform bacteria were found associated with the nitrogen fixing blue-green alga, Anabaena azollae in the leaf cavity of Azolla caroliniana. Plate counts indicated ca. 7,400±1,900 bacterial cells per mature leaf cavity or approximately 1 bacterial cell for every algal cell. No other type of bacterium was found in these cavities.
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  • 9
    ISSN: 1432-072X
    Keywords: Fluorescent antibody staining ; Nitrogen fixation ; Symbiosis ; Anabaena azollae ; Azolla caroliniana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluorescent antibody staining indicated differences in surface antigenicity in Anabaena azollae cells fresh from the leaf cavities of the fern, Azolla caroliniana, and algae which were isolated and subcultured from this fern. Such results suggest that either changes in antigenicity occur in this phycobiont during culturing or that isolation selects for an antigenically different mutant strain capable of in vitro growth.
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  • 10
    ISSN: 1432-072X
    Keywords: Ammonia assimilation ; Lichen symbioses ; Nitrogen fixation ; 15N kinetics ; Peltigera canina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract On following N2-incorporation and subsequent metabolism in the lichen Peltigera canina using 15N as tracer, it was found, over a 30 min period, that greatest initial labelling was into NH 4 + followed by glutamate and the amide-N of glutamine. Labelling of the amino-N of glutamine, aspartate and alanine increased slowly. Pulse-chase experiments using 15N confirmed this pattern. On inhibiting the GS-GOGAT pathway using l-methionine-dl-sulphoximine and azaserine, 15N enrichment of glutamate, alanine and aspartate continued although labelling of glutamine was undetectable. From this and enzymic data, NH 4 + assimilation in the P. canina thallus appears to proceed via GS-GOGAT in the cyanobacterium and via GDH in the fungus; aminotransferases were present in both partners. The cyanobacterium assimilated 44% of the 15N2 fixed; the remainder was liberated almost exclusively as NH 4 + and then assimilated by fungal GDH.
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  • 11
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    Archives of microbiology 135 (1983), S. 103-109 
    ISSN: 1432-072X
    Keywords: RNA polymerase ; Transcription ; Nitrogen fixation ; Symbiosis ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA-dependend RNA polymerase (EC 2.7.7.6) from Rhizobium japonicum was purified. The subunit structure was found to be ββ′α2σ, with the following apparent molecular weights determined by electrophoresis: M r (β and β') 150,000 each, M r (σ) 96,000, M r (α) 40,000, M r (holoenzyme) 490,000, M r (core enzyme) 380,000. The recovery of σ was 28%. RNA polymerase from aerobically grown R. japonicum cells and from nitrogen-fixing cells have the same electrophoretic properties suggesting that no chemical modification of the enzyme occurs when cells undergo this metabolic differentiation. The enzyme is Mg2+-dependent, rifampicin-sensitive, and has optimal activity at alkaline pH (8–10) and at 35–40° C. It binds strongly to bacteriophage T7 promoters, weakly to antibiotic resistance genes, and not at all to cloned R. japonicum nif DNA. Preliminary in vitro transcription experiments, including nif DNA as template, revealed that additional factors may be required for selective transcription from promoters.
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  • 12
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    Archives of microbiology 136 (1983), S. 219-221 
    ISSN: 1432-072X
    Keywords: Xanthobacter ; Nitrogen fixation ; Oxygen sensitivity ; Nitrogen metabolism ; Glutamine synthetase ; Glutamate synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract N2-fixation was investigated in the chemoautotrophic hydrogen bacterium Xanthobacter H4-14. N2-fixing batch cultures of this organism could only be grown at pO2 values of around 0.02 bar, and in continuous culture dissolved oxygen tensions above 16 μM were found to inhibit N2-fixation. Xanthobacter H4-14 utilized a variety of amino acids, nitrate and ammonia as nitrogen sources. Cell-free extracts from steady-state continuous cultures of ammonia grown, nitrate grown and N2-fixing Xanthobacter were assayed for the presence of ammonia assimilation enzymes. No alanine dehydrogenase or glutamate dehydrogenase activity was detected. Ammonia was assimilated exclusively via the glutamine synthetase/glutamate synthase pathway, irrespective of the extracellular concentration of ammonia.
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  • 13
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    Archives of microbiology 124 (1980), S. 161-167 
    ISSN: 1432-072X
    Keywords: Nitrogen fixation ; Photosynthesis ; Green alga ; Chl a and b ; DCMU ; Light and O2 dependency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A unicellular alga which can grow in the light without a combined nitrogen source was isolated from a hot spring. The cells were almost spherical, usually 5–10 μm in diameter. Absorption spectra of the watersoluble pigments and of the acetone-extracted ones revealed the existence of chlorophyll a and b and the absence of phycobilins. Thin sections examined by electron microscopy revealed an eukaryotic organization with features typical of the coccoid green algae (the Chlorococcales). Cells divided by internal cytokinesis and subsequent liberation of daughter cells from the parental wall, in a way similar to Chlorella. The alga reduced acetylene to ethylene and incorporated 15N2 into cell protoplasm when incubated in a low oxygen atmosphere. Nitrogenase activity was light-dependent, microaerophilic and thermophilic. Although the association of symbiotic nitrogen fixing prokaryotes with the cells may still be possible, any such organisms have not so far been detected.
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  • 14
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    Archives of microbiology 127 (1980), S. 115-118 
    ISSN: 1432-072X
    Keywords: Proteus mirabilis ; Serratia marcescens ; Erwinia herbicola ; Nitrogen fixation ; nif genes ; his genes ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plasmid pRD1, an R plasmid of the P incompatibility group which carries his and nif genes from Klebsiella pneumoniae in addition to drug resistance markers derived from RP4, was transferred to His- mutants of Serratia marcescens, Erwinia herbicola and Proteus mirabilis. His+ transconjugants were obtained at low but different frequencies according to recipient genus. Transconjugants all acquired the drug resistance, and were Nif+ in S. marcescens and E. herbicola, having acetylene-reducing activities of the same order of magnitude as the parent K. pneumoniae and fixing 15N2. No evidence for nif expression in P. mirabilis transconjugants was obtained though the nif genes were present.
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  • 15
    ISSN: 1432-072X
    Keywords: Nitrogen fixation ; Nitrogenase regulation ; Glutamine synthetase ; Methionine suofoximine ; Rhodospirillaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methionine sulfoximine (MSX), an irreversible inhibitor of glutamine synthetase of Rhodopseudomonas palustris restored nitrogenase activity to cells in which nitrogenase had been completely inhibited by ammonia switch-off. After addition of MSX, there was a lag period before nitrogenase activity was fully restored. During this lag, glutamine synthetase activity progressively decreased, and near the time of its complete inhibition, nitrogenase activity resumed. Nitrogenase switch-off by ammonia thus required active glutamine synthetase. Glutamine itself caused nitrogenase inhibition whose reversal by MSX depended on the relative ratio of MSX to glutamine. Unlike ammonia, glutamine inhibited nitrogenase under conditions where glutamine synthetase activity was absent. This indicates that glutamine is the effector molecule in nitrogenase switch-off, for instance by interacting with the enzymatic system for Fe protein inactivation. The effects of glutamine and MSX were also dependent on the culture age. Possible explanation for this and for the competitive effects are a common binding site within the regulatory apparatus for nitrogenase, or, in part, within a common transport system. Some observations with MSX were extended to Rhodopseudomonas capsulata and agreed with those in R. palustris.
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  • 16
    ISSN: 1432-072X
    Keywords: Azotobacter vinelandii ; Continuous culture ; Oxygen control ; Nitrogen fixation ; Respiratory protection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Azotobacter vinelandii strain OP was grown in continuous culture at various dissolved oxygen concentrations of air (100% air saturation of the medium=225 ±14 μM O2). Sucrose was added as carbon source and either dinitrogen or ammonia as nitrogen sources. Irrespective of the nitrogen source steady state cultures showed the following general responses with dissolved oxygen concentrations increasing from about 1% to 30% air saturation: (i) cell protein levels, (ii) the amount of cell protein formed per sucrose consumed as well as (iii) nitrogenase activity decreased by at least a factor of two while (iv) cellular respiration increased. At higher oxygen concentrations the parameters changed only slightly, if at all. Increasing the sucrose concentration in the inflowing medium (s R) from 3 g/l to 15 g/l increased the total level of cellular respiration with nitrogen-fixing cultures but was more pronounced with ammonium-assimilating cultures. With nitrogen-fixing cultures cell protein levels increased five-fold while the ratio of protein formed per sucrose consumed as well as cellular nitrogenase activity remained unaffected. With ammonium-assimilating cultures the cell protein level was only doubled and the level of cell protein formed per sucrose consumed was decreased at the higher s R. Increasing the dilution rate at a constant oxygen concentration of 45% air saturation resulted in an almost parallel increase of both cellular respiratory and nitrogenase activity at low and moderate dilution rates. At high dilution rates nitrogenase activity increased steeply over the respiratory activity. Nitrogen-fixing cultures adapted to various oxygen concentrations were subjected to oxygen stress by increasing the oxygen concentration for 7 min. In all cases, this resulted in a complete inhibition (‘switch-off’) of nitrogenase activity. Upon restoration of the original oxygen concentration nitrogenase activity returned to a decreased level. The discussion arrives at the conclusion that some of the results are incompatible with the concept of respiratory protection of nitrogenase.
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  • 17
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    Archives of microbiology 136 (1983), S. 81-83 
    ISSN: 1432-072X
    Keywords: Ammonia production ; Anabaena ; Cyanobacteria ; Nitrate reductase ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the filamentous heterocystous cyanobacterium Anabaena sp. strain ATCC 33047 dinitrogen fixation and nitrate reduction are mutually exclusive processes. Nitrate promotes nitrate reductase synthesis and represses nitrogenase formation. Inhibition of ammonium assimilation by l-methionine-d,l-sulfoximine (MSX) alleviates the repressive effect of nitrate on nitrogenase synthesis, thus indicating that the nitrate effect is indirect through metabolites generated from the ammonium derived from nitrate reduction. In MSX-treated cells both nitrate reduction and dinitrogen fixation take place simultaneously, although at different sites of the filament, without any apparent competition for the required reducing power. The MSX-treated Anabaena cells generate ammonium from both nitrate and dinitrogen, simultaneously.
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  • 18
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Ultrastructure ; Nitrogen fixation ; Water stress ; Taxonomy ; DNA ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two strains of desiccation-tolerant coccoid cyanobacteria, Chroococcus S24, a marine form, and Chroococcus N41, a cryptoendolith isolated from a hot-desert rock, have been characterized. The mol % DNA base compositions of the strains are 47.1 and 48.9% respectively. Plasmid DNA was not detected in either strain. The pigment contents and nutritional characteristics of the strains are identical. Both lack phycoerythrinoid pigments and, in culture, behave as slow-growing halotolerant marine forms with elevated requirements for Na+, Cl−, Mg2+ and Ca2+. Sucrose was the only carbon source of those tested that supported photoheterotrophic growth. Each strain synthesizes nitrogenase under anaerobic conditions but not in air. Morphologically the two strains are indistinguishable. They are considered to be independent isolates of the same cyanobacterial species. Chroococcus N41 was studied in detail with the electron microscope. When brought to equilibrium at matric water potentials of-168 MPa and lower (to-673 MPa=c0.12a w) the protoplast shrinks, but the cells maintain the same size and diameter as those at-2,156 kPa (MN medium; control); the sheath expands and remains attached to the cell wall outer membrane by fibrils. The cell wall, cell membrane, thylakoid membranes, cyanophycin granules and carboxysomes appeared intact in desiccated cells.
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  • 19
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    Oxidation of metals 14 (1980), S. 325-336 
    ISSN: 1573-4889
    Keywords: oxidation ; cyclic ; spalling ; stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The effect of thermal shock on the spalling of oxides from the surfaces of several commercial alloys was determined. The average cooling rate was varied from approximately 240°C/sec to less than 1.0°C/sec during cyclic oxidation tests in air. The tests consisted of 100 cycles of 1 hr at the maximimum temperature (1100 or 1200°C). The alloys were HOS-875, TD-Ni, TD-NiCrAl, In-601, In-702, and B-1900 plus Hf. All of these alloys exhibited partial spalling within the oxide rather than total oxide loss down to bare metal. Thermal shock resulted in deformation of the metal, which in turn resulted, in most cases, in change of the oxide failure mode from compressive to tensile. Tensile failures were characterized by cracking of the oxide and little loss, while compressive failures were characterized by explosive loss of platelets of oxide. This behavior was confirmed by examination of mechanically stressed oxide scales. The thermally shocked oxides spalled less than the slow-cooled samples with the exception of TD-NiCrAl. This material failed in a brittle manner rather than by plastic deformation. The HOS-875 and the TD-Ni did not spall during either type of cooling. Thus, the effect of thermal shock on spalling is determined, in large part, by the mechanical properties of the metal.
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  • 20
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    Plant molecular biology 2 (1983), S. 231-237 
    ISSN: 1573-5028
    Keywords: stress ; differentiation ; plant cell cultures ; heat shock
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pattern of proteins synthesized by carrot cells after heat-shock was analyzed by SDS-polyacrylamide gel electrophoresis. A study was made of the time of appearance and disappearance of the heat-shock bands and a comparison was made of the heat-shock proteins present in cell extracts obtained from cell suspensions, callus, protoplasts and the different stages of somatic embryogenesis. From this comparison a number of differences and hence of stage-specific markers could be seen. This type of analysis was also carried out in the presence of α-amanitin in order to get information on the type of control of heat-shock protein synthesis. It turned out that in all stages after the initial globular stage, α-amanitin does not alter the heat-shock protein pattern suggesting pre-existence of the relative mRNA's.
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  • 21
    ISSN: 1432-2048
    Keywords: Glutamine synthetase ; Leghaemoglobin ; Nitrogenase ; Nitrogen fixation ; Phaseolus ; Rhizobium
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    Topics: Biology
    Notes: Abstract The activities of glutamine synthetase (GS), nitrogenase and leghaemoglobin were measured during nodule development in Phaseolus vulgaris infected with wild-type or two non-fixing (Fix-) mutants of Rhizobium phaseoli. The large increase in GS activity which was observed during nodulation with the wild-type rhizobial strain occurred concomitantly with the detection and increase in activity of nitrogenase and the amount of leghaemoglobin. Moreover, this increase in GS was found to be due entirely to the appearance of a novel form of the enzyme (GSn1) in the nodule. The activity of the form (GSn2) similar to the root enzyme (GSr) remained constant throughout the experiment. In nodules produced by infection with the two mutant strains of Rhizobium phaseoli (JL15 and JL19) only trace amounts of GSn1 and leghaemoglobin were detected.
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  • 22
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    Bulletin of experimental biology and medicine 89 (1980), S. 256-258 
    ISSN: 1573-8221
    Keywords: stress ; contractile function of the heart ; calcium transport
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    Topics: Biology , Medicine
    Notes: Abstract The effect of changes in the Ca++ concentration in the perfusion fluid on function of the isolated heart of control rats and of rats exposed to emotional pain-induced stress (EPS) was studied. EPS was shown to cause a sharp increase in the intensity of the response of the animals' heart to a change in Ca++ concentration in the perfusion fluid: depression of the contractile function in response to a decrease in Ca++ concentration and an increase in the basic parameters of the contractile function in response to an increase in Ca++ concentration after EPS were considerably more marked than in the control. It is suggested that the increased dependence of the heart of animals subjected to EPS is the result of damage to membrane Ca++ transport mechanisms in the myocardial cells.
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    Bulletin of experimental biology and medicine 90 (1980), S. 892-894 
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    Keywords: myocardium ; enderpines ; stress
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  • 24
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    Bulletin of experimental biology and medicine 90 (1980), S. 1651-1653 
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    Keywords: stress ; peroxidation of lipids ; γ-hydroxybutyric acid ; antioxidants
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    Bulletin of experimental biology and medicine 89 (1980), S. 432-435 
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    Keywords: T- and B-lymphocytes ; stress
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  • 26
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    Bulletin of experimental biology and medicine 90 (1980), S. 972-973 
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    Keywords: stress ; chromosomal aberrations ; hypokinesia ; phenazepam
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  • 27
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    Bulletin of experimental biology and medicine 90 (1980), S. 1654-1657 
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    Keywords: steroids ; stress ; hypokinesia
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    Bulletin of experimental biology and medicine 95 (1983), S. 22-26 
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    Keywords: ultrastructure ; myocardium ; stress ; catecholamines
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    Bulletin of experimental biology and medicine 95 (1983), S. 27-30 
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    Keywords: stress ; myocardium ; anoxia ; reoxygenation
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  • 30
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    Bulletin of experimental biology and medicine 95 (1983), S. 137-140 
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    Keywords: ultrastructure ; adrenal medulla ; stress
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    Bulletin of experimental biology and medicine 95 (1983), S. 170-172 
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    Keywords: stress ; myocardium ; hypoxia
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  • 32
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    Bulletin of experimental biology and medicine 95 (1983), S. 167-169 
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    Keywords: atrium ; stress ; infarct ; phospholipases ; chloroquine
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    Bulletin of experimental biology and medicine 95 (1983), S. 351-353 
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    Keywords: blood plasma ; stress ; radioimmune method
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  • 34
    ISSN: 1573-8221
    Keywords: stress ; cytochromes P-450 and b5 ; demethylase activity ; lipid peroxidation
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  • 35
    ISSN: 1573-8221
    Keywords: atrium ; stress ; myocardial infarct ; lipid peroxidation ; actioxidants
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    Bulletin of experimental biology and medicine 95 (1983), S. 757-759 
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    Keywords: rats ; stress ; ischemia ; reperfusion ; contractile function of the heart ; cardiac output
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    Bulletin of experimental biology and medicine 95 (1983), S. 763-766 
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    Keywords: stress ; contractile function of the heart ; glucocorticoids
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    Bulletin of experimental biology and medicine 96 (1983), S. 1000-1003 
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    Keywords: stress ; cortisol ; injections ; circadian rhythms ; 11-hydroxycorticosteroids
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    Bulletin of experimental biology and medicine 96 (1983), S. 1101-1104 
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    Keywords: serotonin ; S2 receptors ; stress ; behavior
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    Bulletin of experimental biology and medicine 96 (1983), S. 1046-1050 
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    Keywords: stress ; bioelectrical activity ; hormones ; reticular formation
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    Bulletin of experimental biology and medicine 96 (1983), S. 1275-1278 
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    Keywords: stress ; psychotropic drugs ; high-energy compounds ; brain
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    Bulletin of experimental biology and medicine 96 (1983), S. 1414-1416 
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    Keywords: stress ; α-adrenoblocker phentolamine ; β-adrenoblocker inderal
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    Bulletin of experimental biology and medicine 96 (1983), S. 1348-1351 
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    Keywords: monoamines ; analgesia ; stress ; auricular electroacupuncture
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  • 44
    ISSN: 1573-8221
    Keywords: stress ; myocardium ; glycogen ; uridine ; extensibility
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  • 45
    ISSN: 1573-8221
    Keywords: atrium ; myocardium ; stress ; adaptation
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    Bulletin of experimental biology and medicine 96 (1983), S. 1679-1681 
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    Keywords: myocardial infarction ; atrium ; gamma-hydroxybutyric acid ; stress
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    Bulletin of experimental biology and medicine 96 (1983), S. 1692-1695 
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    Keywords: stress ; psychotropic drugs ; oxidative phosphorylation ; brain
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    Bulletin of experimental biology and medicine 96 (1983), S. 1716-1718 
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    Keywords: stress ; depression ; contractility of the heart
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    Bulletin of experimental biology and medicine 96 (1983), S. 1723-1726 
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    Keywords: stress ; adrenal gland ; phenazepam ; sodium hydroxybutyrate
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    European journal of plant pathology 89 (1983), S. 265-273 
    ISSN: 1573-8469
    Keywords: tobacco ; virus infection ; ethylene ; salicylic acid ; stress ; cycloheximide ; actinomycin D
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pathogenesis-related proteins (PRs) are induced in tobacco and other plant species by both biotic and abiotic agents, comprising necrotizing and non-necrotizing viruses, viroids, fungi, bacteria, specific physiological conditions and a variety of chemicals. Both ethephon and the natural precursor of ethylene, 1-aminocyclopropane-1-carboxylic acid, are good inducers and induction under all conditions investigated so far appears to be mediated by ethylene, except treatment with benzoic acid or its derivatives salicylic acid, aspirin, and 2,6-dihydroxybenzoic acid. Whereas the production of ethylene appears to result from a general reaction to stress, the mechanism by whicho-hydroxylated benzoic acids induced PRs is different. In ‘Samsun NN’ tobacco, tobacco mosaic virus (TMV) and ethephon induced PRs in both the treated and the untreated leaves at 20°C, but not at 32°C. However, salicylic acid induces PRs only in the treated leaves, but is as effective at 32°C as it is at 20°C. It has been proposed, therefore, that ethylene leads to the temperature-sensitive synthesis of a, presumably aromatic, compound that mimics the action of salicylic acid and functions as the natural inducer of PRs. The induction of PR 1a and 1b by salicylic acid or ethephon is blocked by cycloheximide but not by actinomycin D, whereas their accumulation upon TMV infection is inhibited up to 50% by actinomycin D. Actinomycin D similarly inhibits ethylene production in TMV-infected tobacco, supporting a role of ethylene in the induction of PRs in tobacco and indicating that ethylene acts by regulating the translation of the PR-mRNAs constitutively present but not translated in non-stimulated plants.
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  • 51
    ISSN: 1573-5036
    Keywords: Groundnut fertilization ; Nigeria ; Nitrogen fixation ; Nodulation ; Nutrient uptake ; Tropical soils
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract It was observed in a long-term fertility study that P application results in better nodulation and seed yield. The N yield per plant increased significantly with P, K, and B application, but the promoting effect of K and B on N content per plant was not reflected in the final yield. Data on mean nutrient uptake indicate that about 63% of N, 68% of P, 23% of K, 4% of Ca and 24% of Mg were present in the kernels, the remainder being in the haulm and shells. Soil will be exhausted fast if the nutrient rich groundnut residues are completely exported from the field and not compensated by adequate fertilizer application. Judicious use of fertilizers on groundnut has been shown to yield more than 100 kg of biologically fixed N per hectare.
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  • 52
    ISSN: 1573-5036
    Keywords: Clover ; Fertilizer ; Lotus ; Nitrogen fertilizer ; Nitrogen fixation ; Nitrogen source ; Nodulation ; Trifolium repens
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Studies under growth cabinet conditions investigated the effect of source and concentration of nitrogen and timing of nitrogen application on the growth and nitrogen fixation byLotus pedunculatus cv. Maku andTrifolium repens cv. S184. KNO3, NaNO3 and NH4NO3 were added at transplanting at the following rates: 3.33, 7.78 and 13.33 mg N/plant. KNO3 was added at 3.33 and 7.78 mg N/plant at 0, 6, 12, 18, 24 or 30 days after transplanting. Lotus shoot weight increased with all increasing nitrogen sources but clover only responded to KNO3 and NaNO3. The root weight of both species increased with increasing KNO3 and NH4NO3. The percentage increase in lotus and clover shoot growth was greater than that of root growth when KNO3 was added within a week of transplanting. Increases in growth by both species resulted from added nitrogen except with lotus when NaNO3 was applied where increased nitrogen fixation also contributed to increased growth. Weight and number of effective nodules on both species were increased with 3.33 mg N per plant as KNO3 but nitrogen fixation was not affected. Addition of 13.33 mg N as NaNO3 reduced weight and number of effective nodules in both species and also nitrogen fixation by lotus. KNO3 increased growth and nodulation of both species when applied within one week after transplanting. Nodulated lotus plants responded to KNO3 by increasing growth but not nodulation. KNO3 appeared to affect infection and development of nodules on lotus and may affect the growth of existing nodules on clover.
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  • 53
    ISSN: 1573-5036
    Keywords: Moisture stress ; Nitrogen fixation ; Tropical forage legume
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of soil moisture stress on growth, nodulation and nitrogenase activity of two tropical forage legumes,Macroptilium atropurpureum cv. Siratro andDesmodium intortum cv. Greenleaf was studied in a pot experiment. After ten weeks growth, the highest moisture stress (20 per cent water holding capacity) significantly reduced only the top weight of both plants. Moisture stress progressively retarded top growth in the two legumes. Similar trends were also observed in defoliated plants. Moisture stress had little or no effect on the nodulation or nitrogenase activity of the plants.
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    Plant and soil 72 (1983), S. 321-334 
    ISSN: 1573-5036
    Keywords: Leguminous plants ; Nitrogen fixation ; Protein ; Seed ; Yield
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Mineral nitrogen did not increase grain yield and seed protein levels ofVicia faba L. andLupinus luteus L. in field trials and pot experiments. Fixed N2 was substituted by mineral nitrogen in these cases because of inhibition of N2 fixation by mineral nitrogen. Contrary to these results mineral nitrogen increased grain yields and seed protein amounts ofLupinus albus L.,Pisum sativum L., andGlycine max. (L.) Merr. The nitrogen effect was caused at an early stage by saving energy due to inhibition of N2 fixation (measurement of gas exchange by means of IRGA). In case of the N application after flowering grain, yields and seed protein levels increased because the mineral N was an additional nitrogen source for plants. At this stage the plants had ceased fixing atmospheric nitrogen. The high sink activity of growing fruits induced a lack of assimilates in nodules (determined by means of14CO2 application). The N effect was therefore the consequence of the lower assimilate pool for supplying root nodules in these plants in comparison withVicia faba L. andLupinus luteus L. Hence it follows that response to mineral nitrogen can be a criterion for discovering more effective Rhizobium-host combinations.
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    Plant and soil 73 (1983), S. 431-434 
    ISSN: 1573-5036
    Keywords: Nitrogen fixation ; 15N2 diffusion
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary The kinetic of15N2 diffusion has been measured in a system similar to that for the estimation of N2 fixation in plant microorganism associations cultivated in soil. The15N2 enrichment of the soil atmosphere reached an homogenous value one hour after injection of15N2 and is identical to that obtained by calculation, indicating that no adsorption occurs in the soil particles.
    Notes: Résumé La cinétique de diffusion du15N2 est mesurée sur un système identique à ceux pouvant être utilisés pour la mesure de fixation de l'azote chez les associations plantes-microorganismes cultivées sur sol. L'enrichissement homogène de l'atmosphère du sol est obtenu une heure environ après l'injection de15N2 et correspond à l'enrichissement calculé, ce qui indique qu'aucune adsorption n'a lieu dans les particules du sol.
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    Plant and soil 75 (1983), S. 131-138 
    ISSN: 1573-5036
    Keywords: Hydrogen inhibition ; Nitrogen fixation ; Peas ; Relative efficiency ; Rhizobium leguminosarum ; Root nodules
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Experiments were performed to investigate the causes of low relative efficiency, RE, in legume root nodules. Nitrogen fixing activity and RE varied with time of incubation of nodules and with different temperatures and oxygen concentrations. The effects of nitrogen concentration and carbon dioxide concentration were also examined. In each case the RE was inversely related to nitrogen fixing activity; measured by acetylene reduction. Increasing the nitrogen concentration had no effect on either nitrogen fixing activity or RE. Experiments with isolated bacteroids gave higher RE values than the whole nodules from which they were isolated. All the results were consistent with hydrogen inhibition of nitrogenase within the nodule being the cause of low RE.
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    Plant and soil 55 (1980), S. 163-165 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Nitrogen fixation ; Rhizobium
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method is described to demonstrate nitrogen fixation by free-living Rhizobium cells. After aerobic growth in a nutrient solution, the bacteria are centrifuged. Acetylene reduction by the rhizobial cells in the pellet can be measured within a few days. Hydrogen gas frequently stimulates acetylene reduction.
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    Plant and soil 74 (1983), S. 395-406 
    ISSN: 1573-5036
    Keywords: Fix− mutants ; Fix+ revertants ; Macroptilium lathyroides ; Nitrogen fixation ; Rhizobium
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Eight ineffective mutant strains were isolated from N-methyl-N'-nitro-N-nitrosoguanidine mutagenized cultures of cowpea Rhizobium strain 32H1. Strains CR1, CR2, CR3, CR4, CR5 and CR6 induced more, but smaller, nodules than the wild type. With the exception of strain CR2, these mutant strains reduced less than 1% of the amount of acetylene reduced by the wild type, in both the free-living and symbiotic assays. Strain CR2 reduced acetylene in the free-living assay but not in the symbiotic assay. Strains CR7 and CR8 responded variably (5–20% of the wild type) in free-living and symbiotic acetylene reduction assays. Nodules also varied from small white to normal-sized pink nodules. The phenotypic characteristics of the mutant strains were consistant with all leguminous plants tested and were stable upon reisolation from nodules. Fully effective revertants were selected from 4 of the ineffective mutant strains by the use of the leguminous plant,Macroptilium lathyroides. Serology, patterns of resistance to anti-bacterial agents, phage-typing, and antibiotic resistance markers were used to confirm strain identification.
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    Plant and soil 72 (1983), S. 95-105 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Inga jinicuil ; Nitrogen fixation ; Nodulation ; Nodule biomass ; Nutrients ; Shading ; Tropics ; Woody legume
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A pot experiment was conducted to assess the effects of different fertilizers and soil shading on nodulation and acetylene reduction ofInga jinicuil seedlings. Initially, seedlings produced maximum nodule biomass when grown with high levels of phosphorus but reduced the most acetylene under intermediate phosphorus fertilization. These response differences, however, gradually diminished with age, being negligible when the seedlings were a year old. Nitrogen fertilization inhibited nodulation and acetylene reduction throughout the experiment. Potassium did not significantly affect nodulation, but low levels of potassium stimulated, and high levels inhibited acetylene reduction activity relative to unfertilized control plants. Neither magnesium nor molybdenum affected nodulation or acetylene reduction. Soil shading resulted in decreased nodule biomass and less nitrogen-fixing activity during summer months. However, the data suggest that shading may favour nitrogen fixation in colder periods by moderating soil temperatures. These results confirm findings from an earlier field study and show that nodulation and nitrogen-fixing activity by leguminous trees is influenced by the types and amounts of nutrients supplied. This suggests that the quantity of nitrogen fixed by leguminous shade trees in coffee plantations may be amenable to manipulation through simple management techniques.
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    Plant and soil 75 (1983), S. 309-342 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Amino-acid composition ; Chromosome number ; Cross-inoculation ; Growth various substrates ; Medium composition ; Nitrogen fixation ; Parasponia parviflora ; Root nodule ; Temperate Rhizobium species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Cross-inoculation experiments withParasponia parviflora plants and a large number of Rhizobium strains isolated from legumes, mainly of temperate origin, showed that strains ofRhizobium trifolii andR. lupini could produce root nodulation in Parasponia. Root nodulation was also obtained with some tropical Rhizobium isolates such as those from Arachis and Albizzia, but not withRhizobium japonicum strains. In addition, strains ofR. leguminosarum, R. phaseoli andR. meliloti produced abnormal root nodules or pseudo-root nodules in Parasponia seedlings. All root nodules induced in Parasponia by foreign Rhizobium species lacked, however, nitrogenase activity. Testing the reverse combination Parasponia-Rhizobium on legumes yielded with regard to root nodulation negative results, except withVigna sinensis and twoMacroptilium species (M. atropurpureum andM. lathyroides). The root nodules produced in the latter legumes showed nitrogenase activity.Trema cannabina seedlings inoculated with Parasponia-Rhizobium invariably did not produce root nodules. The feasibility of Parasponia to accept foreign rhizobia bacteria or to produce pseudo-nodulation is probably genetically determined. In a karyological study it was shown thatParasponia parviflora had the chromosome number 2n=20. Growth experiments revealed thatP. parviflora can be cultivated in water culture, perlite and in soil (pot) culture. It can also be grown in agar tubes, although growth under these conditions is sometimes rather variable. Nitrogenase (C2H2) tests showed that water-culture root nodules were 5–10 times less active than those from perlite culture, soil or agar tubes. On the whole, nitrogenase activity of root nodules grown on the various substrates and receiving different treatments, was rather variable.Vigna sinensis plants inoculated with Parasponia-Rhizobium showed on basis of fresh nodule weight a 3–4 times higher nitrogenase activity as compared with the same strain on Parasponia. However, as shown by acetylene reduction tests of nodule slices, the main activity of mature Parasponia root nodules is situated in the apical part of the root nodule. On fresh weight basis the nitrogenase activity of this nodule section is of the same order as that of leguminous root nodules. Intact Parasponia plants showed higher nitrogenase activities than excised root nodules. The maximal nitrogen fixation rate ofParasponia parviflora plants was 20–25 μmol. C2H4·g−1 fresh weight nodule tissue.h−1, which value is comparable to that of legumes and higher than estimates obtained by Parasponia in the field. No decrease of the nitrogen-fixation rate was observed in Parasponia plants tested in a diurnal cycle during the 12-hours dark period. Amino-acid analyses of Parasponia root nodules showed large quantities of aspartic acid, asparagine, glutamic acid and glutamine suggesting a role of these amino acids in the nitrogen fixation or transport processes. In contrast to actinorhizal root nodules Parasponia root nodules do not contain citrulline. The latter amino acid was, however, found—although in rather low concentration—inVigna sinensis andMacroptilium atropurpureum nodules obtained withParasponia-Rhizobium.
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  • 61
    ISSN: 1573-5036
    Keywords: Genetic control ; Nitrogen fixation ; Pisum sativum L. ; Rhizobium Maternal effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In pea cv. Afghanistan a recessive gene sym 6 prevents the full expression of nitrogenase activity in root nodules, induced byRhizobium leguminosarum strain F 13. In contrast, nitrogenase activity is fully expressed in pea cv. Iran. A comparison of the reciprocal hybrids of these two plants showed that the size of the plant was determined by the mother plant (maternal effect). Therefore the shoot weight and the total amount of nitrogen fixed are not suitable as parameters for a genetic analysis. The % nitrogen of the shoot and the specific activity of the nodules per gram of nodules are more reliable, but for practical purposes the specific activity of the nodules expressed per gram of shoot tissue can be used.
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  • 62
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Glycine max L. ; Nitrogen fixation ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A system for employing open-ended root chambers to measurein situ acetylene reduction rates under field conditions is described. Gas mixtures containing about 2 mbar acetylene were continuously flowed through the chambers providing a continuous record of acetylene reduction. These chambers have been used to measure acetylene reduction rates of soybeans during three growing seasons. The system has proved to be reliable with a high degree of precision. The large amount of plant-to-plant variability observed in N2 fixation research has been confirmed by the data collected with this system. However, such variability in physiological studies can be reduced by using a non-destructive system to compare the response of an individual plant with its rates before treatment.
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  • 63
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    Plant and soil 73 (1983), S. 117-128 
    ISSN: 1573-5036
    Keywords: Light quality ; Nitrogen fixation ; Trifolium repens ; White clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The possibilities of using light quality treatments to gain an understanding of the mechanisms controlling the allocation of photosynthate for symbiotic nitrogen fixation were studied. White clover (Trifolium repens) plants were grown at the same photon irradiance in red, blue and green light treatments. Growth, nodulation and the carbon/nitrogen economies of the plants were measured. Both photosynthetic rates per unit leaf area and shoot-root ratios were affected by the treatments. However, the carbon/nitrogen economies of the plants and the fraction of the total plant weight allocated to nodule growth were unaffected.
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  • 64
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    Plant and soil 73 (1983), S. 151-153 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Beijerinckia ; C3 and C4 plants ; Nitrogen fixation ; Phyllosphere ; Wettability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nitrogen fixation on leaf surfaces of sugarcane, sorghum, ragi, bamboo and mulberry plants was determined by using acetylene reduction assay. The data revealed varying levels of positive nitrogenase activity on the surfaces. Beijerinckia was observed to be the predominant diazotroph on the leaves. No correlation between fixation rates and C3 or C4 plant species was discerned. The possiblity of improving phyllosphere nitrogen fixation has been discussed in light of the above observations.
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  • 65
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    Plant and soil 73 (1983), S. 299-305 
    ISSN: 1573-5036
    Keywords: Nitrogen fixation ; Rhizobium trifolii populations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effectiveness ofRhizobium trifolii isolates from five locations in southern Britain representing contrasting soil types has been examined with five white clover varieties. The average effectiveness of Rhizobium isolates varied considerably as did the average productivity of plant varieties. The largest differences were, however, associated with Rhizobium population × plant variety interactions. These were often large enough to reverse relative yield differences between white clover varieties. The implications of these results for improving clover productivity in nitrogen fixation are discussed.
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  • 66
    ISSN: 1573-1561
    Keywords: Helianthus annuus ; Amaranthus retroflexus ; allelopathy ; chlorogenic acid ; seedling growth ; plant debris ; stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract When added to soil,Helianthus annuus debris from plants grown under various nutrient stresses in the greenhouse and field had a significant negative effect onAmaranthus retroflexus seedling dry weight production. The regression models for dry weight versus total phenolic compounds and the variation in N, P, and K contents of theHelianthus debris were significant. It was possible to partially simulate debris inhibition ofA. retroflexus seedling dry weight production when chlorogenic acid alone was added to the soil instead ofHelianthus debris. The inhibition ofAmaranthus growth byHelianthus debris and chlorogenic acid was not evident when nutrient solution was applied to the soil.
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  • 67
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    Plant and soil 54 (1980), S. 33-44 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Harwood forests ; Nitrogen cycle ; Nitrogen fixation ; United States ; Wood litter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nitrogen-fixing activity in hardwood forests of the northeastern United States occurred in wood litter, greater than 2 cm in diameter. Activity in large dead wood was independent of species, in the case of deciduous wood litter, but was restricted to partially decayed wood with a high moisture content. Maximum rates of activity were observed in the summer months, minimum rates in the winter. Evidence from six stands of varying ages showed that fixation in large wood litter occurred in only 25% of the samples assayed. Fixation was highest in the youngest, 4 years, and oldest, over 200 years, stands; being about 2 kg/ha/yr. The quantity of nitrogen fixed appears to be related to the biomass of dead wood. Large amounts of wood litter in the youngest stands were from slash left after cutting. As the supply of slash is exhausted by decay, nitrogen fixation decreases, with a low around year 20. Fixation then gradually increases as natural thinning adds wood to the litter compartment. Apparently, the amount of nitrogen fixed in dead wood the first 20 years following clearcutting can only replace a modest fraction of the amount lost as a result of the cutting and product removal. Finally, the results indicate that nitrogen fixation in wood litter does not equal nitrogen fixation in a northern hardwood forest calculated using a mass balance approach, suggesting that additional nitrogen inputs exist.
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  • 68
    ISSN: 1573-5036
    Keywords: Acid-sensitive step ; Nitrogen content ; Nitrogen fixation ; Pisum sativum ; Rhizobium leguminosarum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Experiments were performed to measure the pH-sensitive steps in nodulation and symbiotic fixation byPisum sativum and isolate RP-212-1 ofRhizobium leguminosarum. An aeroponic system with rigorous pH control was used to obtain numerous effective nodules. After exposure to various pH levels, the following responses were measured: (1) legume root growth and development, (2) survival and growth rate of a single effective bacterial isolate, (3) degree of nodulation, (4) rate of nitrogen fixation, (5) plant biomass, and (6) nitrogen content of plants. Both bacterial growth and root development were adequate at all pH levels from 4.4 to 6.6, but efficient nodulation and nitrogen fixation did not occur at pH 4.8 and below. The processes required for symbiosis were about 10 times as sensitive to acidity as either bacterial growth or root growth alone. Nodulation was the most acid-sensitive step.
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  • 69
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    Plant and soil 56 (1980), S. 335-340 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Field method ; Nitrogen fixation ; Non-destructive ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Direct injection of acetylene into soil around plant roots, followed by determination of ethylene/acetylene ratios in the soil atmosphere has been tested as a rapid, non-destructive method of estimating acetylene reducing activity. In pots of artificial media as well as in field soil, the ratios determined within 10 min. after injection were significantly correlated with the rates of acetylenedependent ethylene production in detached roots. The method may be useful in preliminary screening of large numbers of plant-bacteria combinations.
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  • 70
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Nitrogen fixation ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A modifiedin situ technique for measuring heterotrophic nitrogen fixing (acetylene reducing) activity associated to rice is proposed. Ethylene evolution rates measured in opaque cylinders covering the stems of rice plants which have been cut 10 cm over the water level were found independent of the diurnal cycle. Cutting of the leaves resulted in decreased variation between plants and suppression of the acceleration of ethylene evolution rate after 12 h incubation as compared to intact plants. In both systems ethylene evolved was swept by a current of methane and the molar ratio between methane and ethylene was stabilized after 12 h. Methane evolution rates remained stable during 12 h and more than 24 h in whole plants and cut plants respectively. It is suggested that alteration in the active gas transport system after 12 h incubation under 10% acetylene may lead to erroneous evaluation of the actual ethylene production in the root's environment. The average values of ethylene evolution rates by cut plants between 12 and 24 h of incubation may be used for comparative studies of nitrogen fixing activity associated to flooded rice.
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  • 71
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    European journal of plant pathology 89 (1983), S. 245-254 
    ISSN: 1573-8469
    Keywords: pathogenesis ; stress ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The main properties of ‘pathogenesis-related’ proteins induced inNicotiana species during hypersensitive reactions to different pathogens, as well as by chemical or physical treatments, are listed. These properties are compared with those of similar protein compounds occurring in other plant species in similar circumstances. The plants include cucumber, cowpea,Gomphrena globosa kidney bean,Gynura aurantiaca, tomato, potato, citron and celery. Similarities with other proteins normally occurring in plants, such as proteinase inhibitors, are considered. Analogies and differences with proteins induced in plants by environmental stresses, and with the ‘antiviral factors’ and the ‘inhibitor of viral replication’ occurring inNicotiana species are briefly discussed.
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  • 72
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    Bulletin of experimental biology and medicine 90 (1980), S. 989-991 
    ISSN: 1573-8221
    Keywords: pathological mitoses ; stress ; adrenalectomy ; cell cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 73
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    Bulletin of experimental biology and medicine 90 (1980), S. 1493-1496 
    ISSN: 1573-8221
    Keywords: rat heart ; stress ; γ-hydroxybutyric acid ; ionol ; peroxidation of lipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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