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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Potato research 37 (1994), S. 11-20 
    ISSN: 1871-4528
    Keywords: Wachstumskapazität ; Spross ; Wurzel ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary The following test was carried out in two years: during physiological ageing, samples of 15 tubers of each of eight cultivars were desprouted, planted into a mixture of compost and peat, and placed in darkness at a temperature of 22°C. Averaged over cultivars, the number and length of sprouts increased with increasing physiological age of the seed tubers until a maximum was reached. Beyond this, a slight decline in the number of sprouts was observed (Fig. 1a,b). With advanced physiological age, the diameter (Fig. 1c) and dry-matter content (Fig. 1d) of the sprouts decreased. However, these characteristics could not be used to monitor differences in the rate of physiological ageing between the potato cultivars. The growth capacity (GC=sprout and root mass) (g) per tuber after 20 days at 22°C) showed an optimum in the course of physiological ageing (Fig. 3). Root mass reached optimum levels earlier than sprout mass (Fig. 2) and in most cases also earlier than the total mass (Fig. 3). Despite year-to-year variation cultivar differences in the rate of ageing could be noted from the timing of the GC optima (based on temperature sum), from the maximum values of GC and from the GC values immediately after the end of dormancy. For three pairs of cultivars the differences in rate of physiological ageing—as observed in field trials—were confirmed by differences in growth capacity in the laboratory tests (Figs. 2, 3a, 3c). For one pair of cultivars the laboratory test did not predict the different behaviour in the field (Fig. 3b).
    Notes: Zusammenfassung Die Wachstumskapazität (GC=g Spross- plus Wurzelmasse pro Knolle nach 20 Tagen bei 22°C) beschreibt im Verlauf der physiologischen Alterung eine Optimumskurve. Das Maximum der Wurzelmasse wurde in der Regel früher durchlaufen als das Maximum der Spross- bzw. Gesamtmasse. In 2 Jahren konnten Sortenunterschiede für die Alterungsrate in der Lage des Optimums der GC auf der physiologischen Zeitachse, in der Breite des optimalen Bereiches der GC und in der Höhe der GC direkt nach der Beendigung der Dormanz festgestellt werden.
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  • 2
    ISSN: 1871-4528
    Keywords: Solanum tuberosum ; Solanum vernei ; potato cyst nematodes ; resistance ; maturity ; leaf area ; biomass partitioning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The tolerance toGlobodera pallida of 15 potato genotypes with a high level of resistance toG. pallida was assessed by measuring their tuber yield in heavily infested fields and in nematicide treated strips in the same fields. Tolerance was also assessed in pots in the glasshouse by measuring the total biomass after 35 and 70 days growth in heavilyG. pallida infested soil and in uninfested soil. Large differences in tolerance between the genotypes were observed. High correlations were observed between tolerance assessments made in the field experiments and between field and pot experiments. No relationship was found between tolerance and maturity. Shoot and root dry weight were about equally affected by the nematode.
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  • 3
    ISSN: 1871-4528
    Keywords: cut tubers ; potato mop-top virus ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In trials with potato tubers infected with tobacco rattle virus (TRV), symptoms of spraing in cvs Bellona, King Edward, Maris Bard, Matilda, Sv 82146 and Sv 82149 increased during storage when the tubers were cut. Storage of intact tubers at a constant temperature of 9°C or at fluctuating temperatures (2 weeks at 18°C, 2 weeks at 9°C and 2 weeks at 18°C) did not increase the frequency of symptoms.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Potato research 37 (1994), S. 3-10 
    ISSN: 1871-4528
    Keywords: α-Solanin ; α-Chaconin ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary Five table potato cultivars (Erna, Granola, Gusto, Ulse, Margit) from the same source were examined for their content of α-solanine and α-chaconine during a sixmonths storage period. For the testing and subsequent analysis the tubers were dissected into four kinds of tissues: eye-zone, peel, vascular bundle region, medulla. The total glycoalkaloid content (TGA) in the total tuber mass varied between 2.4 and 8mg per 100g fresh matter depending on cultivar (Fig. 1). Despite significant differences between cultivars (Table 1), all potato cultivars remained within the safe range during the entire storage period. Remarkable differences in TGA per 100g dry matter could be noted in the tuber parts. For the eye-zones a TGA of 164mg was found; the peel region showed 101mg and the vascular bundle zone 8mg. The medulla of the tubers contained only traces of TGA (0.164mg/100g dry matter, Fig. 2). The statistical analysis only revealed significant interactions between cultivars and tuber parts (Table 1), mainly caused by the cultivar Gusto which showed higher contents of α-solanine and α-chaconine in its vascular bundle regions (Figs 3–5). The ratio between amounts of α-solanine and α-chaconine was approximately 1∶1.5, and after eliminating effects of cultivar, storage and tuber parts, the correlation coefficient between amounts was 0.865. This close correlation can be used for breeding. The non-significant, slightly negative effect of the six-months storage period on alkaloid content was noteworthy. It is assumed that only after long storage periods or through inappropriate storage (sprouting of tubers) a significantly higher glycoalkaloid content may be expected.
    Notes: Zusammenfassung Fünf Speisekartoffelsorten wurden während einer sechsmonatigen Lagerung fünfmal auf α-Solanin und α-Chaconin untersucht. Es wurden vier Gewebebereiche der Knolle getrennt überprüft. Der Gesamtglykoalkaloidgehalt betrug im Augenbereich 164, in der restlichen Schale 101 und in der Gefässbündelzone mit äusserem Speichergewebe 8mg/100g. Trockensubstanz. Im restlichen Innenkörper waren nur noch Spuren (0.164mg/100g TS) nachzuweisen. Zwischen den Sorten und diesen Gewebepartien traten statistisch gesicherte Wechselwirkungen auf, die im wesentlichen von einer Sorte verursacht wurden. Während der Lagerung sind die Alkaloidgehalte leicht, aber statistisch nicht gesichert gesunken. α-Solanin und α-Chaconin kamen generell im Verhältnis von etwa 1∶1.5vor; die Korrelation zwischen beiden Formen ist sehr eng (r=0.865). Bezogen auf die gesamte Knolle lagen die Gesamtglykoalkaloide je nach Sorte zwischen 2.4 und 8mg/100g Frischsubstanz und damit im gesundheitlich unbedenklichen Bereich.
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  • 5
    ISSN: 1573-5060
    Keywords: Agrobacterium rhizogenes ; antisense RNA ; granule-bound starch synthase ; Solanum tuberosum ; starch composition ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Granule-bound starch synthase (GBSS) catalyses the synthesis of amylose in starch granules. Analysis of antisense RNA mediated inhibition of GBSS gene expression in large numbers of tubers from in vitro grown, greenhouse grown and field grown transgenic potato plants revealed stable and total inhibition of GBSS gene expression in one clone. In three other transgenic genotypes partial and unstable inhibition was found. In these genotypes both GBSS activity and amylose content were remarkably reduced compared with the non-transformed control genotype. No relationship was found between the level of inhibition of GBSS gene expression and yield and dry matter content.
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  • 6
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; tuber yield ; under water weight ; general combining ability ; special combining ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Tuber yield and dry matter content, measured as under water weight (UWW), of 24 diploid 2x−2x progenies and 12 tetraploid 4x−2x progenies, have been measured in the seedling generation and the first clonal generation. The results were used to evaluate predictions of progeny means, and to compare the general combining ability (GCA) estimated from 2x−2x crosses with the GCA estimated from 4x−2x crosses. Based on parental values and the means of the seedling as well as first clonal generation, the prediction of UWW of the 2x−2x and the 4x−2x progenies was better than that of tuber yield. For GCA similar differences between UWW and tuber yield were found. Three diploid clones were used both for the 2x−2x crosses and the 4x−2x crosses. The ranking of the GCA values, estimated from the 2x−2x crosses, was similar to that obtained from the 4x−2x crosses for UWW in both generations and, to a lesser extent, for tuber yield in the first clonal generation, suggesting, that it might not be necessary to carry out 4x−2x testcrosses to identify diploid clones with superior GCA for tuber yield and dry matter content.
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  • 7
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    Euphytica 53 (1991), S. 151-157 
    ISSN: 1573-5060
    Keywords: Breeding ; coefficient of variation ; competition ; decision support system ; interaction ; optimization ; potato ; response ; selection ; sensitivity ; Solanum tuberosum ; tuber yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A model for optimising single-stage and single-trait selection is presented. The model is based on the expected selection response model, extended with parameters describing the nature of the environment and with variables allowing a manipulation of selection schemes. As an example, the model is used to optimize tuber yield selection in potato breeding. Estimated parameter values have an uncertainty, which may bias the model outcome. Sensitivity analysis shows that the sensitivity to unrealistic parameter values for optimal allocation is less than the sensitivity for the selection response.
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  • 8
    ISSN: 1573-5060
    Keywords: Potato ; protein-DNA-binding ; SDS PAGE ; Solanum tuberosum ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Phenotypic variation, SDS-PAGE and protein-DNA binding were used to determine variation during the in vitro phase of potato plantlets derived from callus and cell suspensions. Of the 27 plantlets assessed. 3 displayed a low or abnormal growth, 16 normal growth which correlated well with the original explant and 9 showed strong or vigorous growth. Differences were not observed in the polypeptide profiles of these plantlets. However distinct differences in the protein-DNA-binding profiles occurred which correlated well with the phenotypic variation observed.
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  • 9
    ISSN: 1573-5060
    Keywords: genetic stability ; Solanum tuberosum ; rDNA ; slow growth ; cryopreservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ribosomal gene (rDNA) probes have been used to assess genomic changes in plants of (1) S. tuberosum cv Desiree subjected to slow growth and (2) S. tuberosum cv Golden Wonder recovered from cryopreservation. Restriction fragment length polymorphisms (RFLPs) were detected in two out of 16 DNA samples extracted from plants derived from slow growth, control plants did not show RFLP differences. Plants recovered from cryopreserved shoot-tips of Golden Wonder were unchanged in their ribosomal gene RFLP profile compared to the untreated controls. The use of rDNA probes as tools to assess stability, and the possible detrimental effects of slow growth, somaclonal variation, cryoprotectants, and freezing injury are discussed.
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  • 10
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    Euphytica 56 (1991), S. 143-148 
    ISSN: 1573-5060
    Keywords: dihaploids ; pollen fertility ; pollen viability ; pollen viability assays ; potato ; Solanum tuberosum ; X-Gal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The main obstacle in breeding potato (Solanum tuberosum L.) dihaploids is the severe limitation of male fertility. To determine pollen viability assays that correlate well to fertility in crosses, results of five different pollen viability assays were compared by correlation analysis with fruit and seed set characters in test crosses, and to pollen tube growth in situ (PL-test). The methods used were: staining the pollen cells with carmino acetic acid (CAA-test); in vitro pollen germination (PG-test); and detection of pollen staining rates after incubation with fluoresceine diacetate (FDA-test), 2-3-5-triphenyle tetrazolium chloride (TTC-test), and 5-bromo-4-chloro-3-indolyle-β-galactoside (X-Gal-test). The results of test crosses and pollen tube growth in situ correlated with the results of all other assays with the following ranking, from highest to lowest: enzyme activity assays (X-Gal-test, FDA-test, TTC-test), in vitro pollen germination (PG-test), and pollen staining by CAA. The newly developed X-Gal-test for monitoring β-galactosidase activity showed the least variation of all assays investigated. Thus, this highly reproducible simple procedure is recommended for male fertility screening.
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  • 11
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; amylose-free mutant ; unreduced gametes ; analytic breeding ; desynapsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using an amylose-free (amf)mutant of diploid potato (Solanum tuberosum), diploid and tetraploid clones with different genotypes at the amf-locus were produced. In order to make use of the diploid material in analytic breeding of amf-potatoes, clones were selected that produced a considerable frequencies of 2n-pollen and 2n-eggs. Successful attempts were made to select normal synaptic as well as desynaptic clones producing 2n-gametes. Based on the phenotype of starch in the microspores, tetraploid clones with nulliplex, simplex and duplex genotypes at the amf-locus were selected. Prospects of using this material in conventional as well as in analytic breeding of potato are discussed.
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  • 12
    Electronic Resource
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    Springer
    Euphytica 54 (1991), S. 245-250 
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; rDNA ; IGS variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A restriction enzyme map of the ribosomal RNA genes (rDNA) in Solanum tuberosum cultivars Golden Wonder and Desiree has been constructed. An heterologous probe pTa 71 containing the rDNA derived from wheat was used to detect and map the corresponding region in potato genomic DNA fragments. rDNA repeats of cultivars Desiree and Golden Wonder are similar with respect to their target sites for restriction enzymes, however, these cultivars have diverged with respect to the length of their intergenic spacer (IGS) sequences. IGS length variants may reflect the different breeding systems for Golden Wonder and Desiree. Furthermore, some of the 25S genes of both cultivars appear to have a 100–150bp insertion/deletion near their 3′ end. The presence of RFLPs (restriction fragment length polymorphisms) in the IGS region could be used as a DNA fingerprint for potato cultivar identification; the ability to recognise IGS length variants may be of significance to potato phylogenetics and breeding.
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  • 13
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; Phytophthora infestans ; late blight ; adventitious regeneration ; somaclonal variation ; tissue culture ; mutation ; maturity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Adventitious regenerants (‘somaclones’) of ‘Bintje’ and their vegetative progeny were screened for field resistance to Phytophthora infestans as follows: the area under the disease progress curve was computed and correlated with resistance rating in ‘Bintje’ and reference varieties. The resistance rating of the somaclones was determined from this relationship. Clones with stable improved field resistance in successive years' trials were detected, however, most of such clones were also maturation mutants. Variation in resistance rating in clone replicates and between years was detected in most clones. The possible basis of the field resistance and reasons for its instability are discussed.
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  • 14
    ISSN: 1573-5060
    Keywords: biomass performance ; performance ratio ; genetic differences ; height increase ; leaf formation rate ; potato ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The main purpose of the research reported in this article was to see if the biomass maintenance of parental potato clones was correlated with the growth and yield of their progeny. Seedling populations with parents of European and of Andean origin were cultivated at a temperature of 10°C during the day and 4°C at night. The biomass maintenance of the parents was defined as the ratio between the total biomass yield at 20°C day/10°C night and the total biomass yield at 10° C day/4°C night. The Andean parents had good maintenance ability at the lower regime compared to the Eurpean parents. Biomass maintenance appears to be hereditary. Significant correlation was found between the dry matter maintenance of the parents and the growth rate of seedlings, especially during the second month after potting. In populations with only Andean or only European parents, differences in height increase and leaf formation rate between seedling populations were correlated to the dry matter maintenance of the parents cultivated at the temperature regime 10/4°C. In the hybrid population between an Andean and an European parent only differences in height increase during the second month after potting correlated well to the average dry matter maintenance of the parents. It appears therefore that the rate of height increase during the second month after potting can be used as a criterion for selection.
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  • 15
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; Nicotiana plumbaginifolia ; asymmetric hybrids ; radiation damage ; electrofusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rate and extent of spontaneous and induced chromosome loss have been determined at the callus level of somatic hybrids of mutants of Solanum tuberosum and Nicotiana plumbaginifolia. AEC (amino ethyl cystein) resistance in potato and Nitrate-Reductase deficiency in N. plumbaginifolia have been used as genetic markers and chromosome morphology as a cytological marker. In this combination, development of hybrid callus was late and slow. Only a limited number of non-regenerable hybrid calli have become available. Chromosome loss could clearly be established in these hybrids from loss of markers and from chromosome cytology. Loss of markers occurred independantly. The best conditions to induce loss of chromosomal material in donor cells by irradiation were found by cytological investigations. A very drastic reduction in chromosome transfer by fusion could be effected by irradiation of plant tissue and subsequent preparation of protoplasts after a few days. Following fusion, hybrid callus was recovered with the potato genome drastically reduced. The amount of loss was deduced from the presence of a few fragments in metaphase cells or from interphase nuclei after in situ hybridization with a repetitive potato DNA probe.
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  • 16
    ISSN: 1573-5060
    Keywords: aneuploidy ; cold resistance ; interspecific somatic hybridization ; morphological characterization ; multivariate analysis ; Solanum tuberosum ; Solanum brevidens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Somatic hybrids between Solanum tuberosum L. cv. Gracia (2n=4x=48) and Solanum brevidens Phil. (2n=2x=24) were produced via fusion of mesophyll protoplasts. Selection of the protoplast derived putative hybrid calli was based on their vigorous growth. Additive isozyme patterns and chromosome numbers as well as the expression of parental morphological characters have proved the hybrid origin of the selected regenerants. Extensive chromosome loss during the regeneration process resulted in aneuploid hybrids with high frequency. Genomic instability could not be detected in these plants during the period of vegetative propagation. Regenerants from hybrid tissues exhibited wide morphological variation especially in tuber formation. The detailed morphological analysis based on the use of multivariate method (principal component analysis, PCA) enabled to identify morphological groups among the hybrid clones. The positioning of hybrid clones in the PCA space could not be correlated with chromosome numbers. The genomic ratio represented by the tetraploid and diploid parents influenced the morphology of somatic hybrid population according to the applied analytical system. Two selected hybrid clones have exhibited an intermediate degree of frost tolerance compared to the parents, based on the recovery of plants from lower buds after freezing of potted plants.
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  • 17
    ISSN: 1573-5060
    Keywords: Solanum sp. ; Solanum tuberosum ; low temperature tolerance ; biomass production ; pollen germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The temperature-related performances of six tetraploid potato clones, two Andean, three European, and one hybrid, were compared by cultivating them in growth chambers under one of two temperature regimes: 10°C day/4°C night or 20°C day/10°C night. Preformance was measured in terms of biomass production and pollen germinability. For dry matter yield at second harvest, the temperature-related performance ratios (performance at 10°C/4°C divided by performance at 20°/10°C) were highest for the Andean clones, intermediate for the Andean/European hybrid clone 2015×S12, and lowest for the European cultivars. The ability of the European cultivars to maintain their normal rates (i.e. rates at 20/10°C) of biomass production when cultivated at low temperatures varied greatly, with clone S3 performing most poorly at 10/4°C. For pollen germinability in vitro, performance ratios were highest for the hybrid clone 2015×S12 and lowest for the European clones. The high tuber yield of hybrid 2015×S12 at 10/4°C can be attributed to its low-temperature tolerance and it being daylength neutral.
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  • 18
    ISSN: 1572-9680
    Keywords: shelterbelts ; wind effects ; tropical north Australia ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An experimental study was conducted on the Atherton Tablelands of tropical north Australia to quantify the shelterbelt benefit to the production of potato (Solanum tuberosum). Wind speed, wind direction and potato yield were measured at various distances from the leeward side of the shelterbelt. Non-linear modelling was used to describe the relationship between potato yield and distance from the shelterbelt. With the developed model, definite integral was used to calculate the net increase percentage of potato yield. Wind speed was greatly reduced by the shelterbelt. Potato yield was increased by 6.7% due to the shelterbelt. It appears that fitting non-linear models is a useful method to determine an accurate net increase of crops from shelterbelts, providing an accurate assessment of the average crop yield on the open areas is achieved.
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  • 19
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    Euphytica 57 (1991), S. 189-194 
    ISSN: 1573-5060
    Keywords: genetic markers ; isozymes ; starch gel electrophoresis ; mutation ; Solanum tuberosum ; line selection ; clonal selection ; potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seven varieties and 57 spontaneous or induced in vitro mutant lines (20 macromutant and 37 micromutant events) of potato were tested by starch gel electrophoresis for ADH, GOT, PGI, PGM, ACO, IDH, MDH and 6PGDH isozymes in tuber extracts. The data showed that in contrast to variety comparisons, the isozyme patterns rarely differentiate mutant lines which have altered morphological traits. But trying to identify isozyme differences in mutants can still be useful for a chimeric structure for GOT-2 alleles in a mutant from Atlantic and a new tuber specific locus for 6PGDH in mutants from Russet Burbank were found.
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  • 20
    ISSN: 1573-5060
    Keywords: potato ; Solanum tuberosum ; breeding ; stress ; yield ; path analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twenty potato cultivars were tested in replicated trials in three seasons. The weather gradually changed from relatively high humidity and cold air temperatures in February to dry and hot in July. Fresh and dry weights, sucrose, reducing sugar and starch contents were recorded on tubers sequentially harvested over four stages during the growing period of each of the three trials. The data were analyzed for genotype-environment interactions by a path regression model which is composed of a main effect and four multiplicative effects. The model fitted well to the observed data for all traits but reducing sugar. Results of dry tuber weight were used to calculate a heat susceptibility index for each of the cultivars. Four components of the index were identified based on the path model. Comparison of sizes of the components provided information on the degree of response to the environmental stress of each of the cultivars during successive stages of growth. High yielding and heat tolerant cultivars were identified based on the experimental results.
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  • 21
    ISSN: 1573-5060
    Keywords: Erwinia carotovora subsp ; atroseptica ; Phytophthora infestans ; PVY ; RAPD ; Solanum brevidens ; Solanum tuberosum ; somatic hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Symmetric somatic hybrids were produced by electrofusion of protoplasts of two dihaploid tuber-bearing potato (Solanum tuberosum L.) lines and Solanum brevidens Phil., a diploid non-tuber-bearing wild potato species. A total of 985 plants was obtained. Verification of nuclear hybridity of putative hybrids was based on additive RAPD patterns, general morphological characteristics and chromosome counts. 53 (90%) calli regenerated into plants which were identified as somatic hybrids. Most of the hybrids were aneuploids at the tetraploid (4×) or hexaploid (6×) level. The 20 hybrids tested expressed a high level of resistance to potato virus Y (PVY N ) characteristic of the S. brevidens parent. Resistance to late blight (Phytophthora infestans (Mont.) de Bary) varied between hybrids, but was on average better than that of the fusion parents. Resistance of hybrids to bacterial stem rot (Erwinia carotovora subsp. atroseptica (van Hall) Dye) was not superior to that of commercial potato cultivars.
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  • 22
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    Euphytica 75 (1994), S. 163-172 
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; mini-tubers ; in vitro culture ; micropropagation ; genetic stability ; field performance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Production of mini-tubers as a source for seed potato was investigated by growing in soil micropropagated plants and micro-tubers produced from micropropagated plants. Cultures of several cultivars were initiated from indexed tubers and multiplied on modified MS medium. Cultures were micropropagated by using a modular system which allowed batch handling. Micropropagated plants produced mini-tubers in glasshouse after 70–115 days of growth in soil. A large proportion of the mini-tubers produced were between 9 and 15 mm diameter. Several factors, e.g., explant number, duration of in vitro culture and genotype influenced mini-tubers production. Micropropagated plants after culture of 86 days or longer produced micro-tubers ca. 2 to 10 mm diameter. Plants, which formed micro-tubers in vitro, produced less number of mini-tubers in soil. Micro-tubers produced 1 to 3 mini-tubers when grown in soil in chain-type paper pots, but produced conventional sized tubers when grown in soil under plastic polytunnel. Mini-tuber number varied widely between potato cultivars; cvs. Bintje and British Queen produced more mini-tubers than the other cultivars. Mini-tubers developed green hard skins when kept in light for 3 weeks, and could be stored in dark at 4° C upto 6 months. In a field trial, small mini-tubers ca. 5–10 mm diameter produced more but smaller tubers than mini-tubers ca. 15–20 mm diameter. The micropropagated plants and the plants grown from mini-tubers were genetically stable, and did not show any morphological aberrations except for one variegated plant among the several thousand produced. It is concluded that the production of mini-tubers by soil planting of micropropagated plants is a rapid and efficient method for producing seed potato tubers.
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  • 23
    ISSN: 1573-5060
    Keywords: Fusarium solani f. sp. eumartii ; culture filtrate ; electrolyte leakage ; potato wilting ; resistance ; toxin ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fusarium solani f.sp. eumartii Carp. Snyder and Hansen (Fusarium eumartii) is a soil inhabitant that induces the so-called Potato Wilt and Stem End Rot disease. Prior to wilting, the pathogen induces peculiar small bronze spots on the leaflets. Failure to isolate F. eumartii from infected leaflets suggests the involvement of a toxin in the disease. The fungus was grown in liquid Richard's medium and thereafter a filtrate was obtained dialyzing (MW cutoff 12,000–14,000) and sterilizing the culture by filtration (0.22 μm). Potato leaves treated with both the pathogen or the filtrate showed symptoms of bronze spots and significantly higher electrolyte leakage when compared to controls. Tomato leaves showed neither bronze spots nor electrolyte leakage after plant inoculation with the pathogen or with the filtrate treatment. Both, the absence of visible symptoms and the lack of electrolyte leakage in tomato could be associated to a certain degree of host specificity of the F. eumartii filtrate towards potato. The filtrate also induced symptoms similar to infections by F. eumartii in adult plants and in vitro plantlets of cultivars Huinkul MAG and Kennebec. Callus responses to the filtrate were related to responses of the cultivars to the pathogen in greenhouse. These results show the potential of the culture filtrate of F. eumartii for use in screening for wilting resistance.
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  • 24
    Electronic Resource
    Electronic Resource
    Springer
    European journal of plant pathology 97 (1991), S. 151-161 
    ISSN: 1573-8469
    Keywords: dry matter partitioning ; Globodera pallida ; organ characteristics ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Potatoes were grown under a permanent rain shelter in mobile containers in soil with or without potato cyst nematodes (Globodera pallida). The plants were subjected to an early drought stress period starting at planting until 43 days after planting, to a late drought stress period starting at 43 days until senescence at 92 days and to a drought control. Dry matter weight and characteristics of leaves, stems, stolons and roots were determined at periodic harvests. The early drought stress and nematode infection affected many plant organ characteristics in similar ways. Numbers of leaves, specific leaf area, plant height, specific stem weight, leaf area ratio, mean tuber weight and harvest index were reduced by both stress factors at early stages of growth. Later on, interactions between both stress factors which influence the development rate of the plants led to more diverse plant reactions. Plants of all treatments rapidly senesced at about 90 days after planting. Uninfected plants had then depleted the soil nutrient supply whereas the plants grown in the inoculated soil senesced as a result of the potato cyst nematode infection.
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