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  • 1
    Electronic Resource
    Electronic Resource
    In vitro bulblet production of Lachenalia (1999)
    Springer
    Plant cell reports 18 (1999), S. 620-624 
    Details
    ISSN: 1432-203X
    Keywords: Key words Commercial propagation ; Bulblets ; In vitro ; Plantlets ; Lachenalia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro bulblet formation and subsequent transplanting of bulblets to soil were studied in order to develop a cost-effective method for the mass production of three Lachenalia varieties. Clumps of adventitious shoots regenerated from leaf explants were used. Bulblet formation was initiated after 2 weeks when shoots were subjected to low temperature (4–15 °C). The size (age) of the adventitious shoot affected the bulblet size, and shoots shorter than 4 mm did not form bulblets. Larger bulblets formed on medium containing 6% sucrose compared to 3% sucrose. Following bulblet initiation, illumination was not necessary for the completion of bulblet formation. Bulblets went into dormancy 3–4 months after they had been initiated or when the culture medium dried out, and they were released from dormancy when the natural night temperatures started to decrease in the late summer. The survival rate of the bulblets after transplanting was directly correlated to the size of the bulblets.The most important factors influencing in vitro bulblet formation of Lachenalia were sucrose concentration, temperature and length of explant shoots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050632
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  • 2
    Electronic Resource
    Electronic Resource
    Adventitious in vitro plantlet formation from immature floral stems of Crinum macowanii (1995)
    Springer
    Plant cell, tissue and organ culture 43 (1995), S. 51-57 
    Details
    ISSN: 1573-5044
    Keywords: Bulbs ; bush lily ; histological study ; regeneration ; shoots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Adventitious shoots and plantlets were regenerated in vitro from floral stem explants of Crinum macowanii (Bak.) (bush lily). The length (age) of the floral stem as well as the orientation and position of the explant disc in the floral stem were the most important factors affecting shoot regeneration. The highest number of shoots were regenerated when immature floral stems of 70–100 mm were used as starting material, using the middle or basal parts of the stem, and orientating the discs with their proximal ends on the medium. Combinations of kinetin (4.65 μM) and either indoleacetic acid (0.57 μM) or naphthaleneacetic acid (0.54 μM), or a combination of benzyladenine (4.44 μM) and 2,4-dichlorophenoxyacetic acid (0.45 μM) resulted in the highest numbers of shoots being regenerated. Although a slight degree of callus formation was noticed on the cut-edges of the discs, shoot formation did not occur via callus, but directly from the floral stem epidermis. Unrooted shoots were rooted on MS-medium containing 0.17 M sucrose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042671
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  • 3
    Electronic Resource
    Electronic Resource
    Variation in phenotype and proteins in plants regenerated from cell suspensions of potato cv. BP1 (1991)
    Springer
    Euphytica 54 (1991), S. 41-44 
    Details
    ISSN: 1573-5060
    Keywords: Potato ; protein-DNA-binding ; SDS PAGE ; Solanum tuberosum ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Phenotypic variation, SDS-PAGE and protein-DNA binding were used to determine variation during the in vitro phase of potato plantlets derived from callus and cell suspensions. Of the 27 plantlets assessed. 3 displayed a low or abnormal growth, 16 normal growth which correlated well with the original explant and 9 showed strong or vigorous growth. Differences were not observed in the polypeptide profiles of these plantlets. However distinct differences in the protein-DNA-binding profiles occurred which correlated well with the phenotypic variation observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00145629
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  • 4
    Electronic Resource
    Electronic Resource
    Regeneration of bulblets from twin scales ofCrinum macowanii in vitro (1993)
    Springer
    Plant cell, tissue and organ culture 33 (1993), S. 133-141 
    Details
    ISSN: 1573-5044
    Keywords: anatomical study ; ancymidol ; bulblet formation ; Crinum macowanii ; in vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tissue culture techniques were applied to study the regeneration and growth of bulblets from bulb scale segments ofCrinum macowanii Bak. (bush- or march lily)in vitro. Shoots were induced on twin scales taken from the basal plate region of flowering-size bulbs on Murashige and Skoog (MS)-medium containing 0–20 mg l−1 NAA and BA and a modified MS medium (MMS medium) containing 1.25 mg l−1 ancymidol (A-RestTM), 0.1 mg l−1 NAA and 0.1 mg l−1 kinetin (ANK). Large bulblets could only be initiated on the latter. Subsequently the bulblets of 5 mm or more in diameter were trimmed and split in half, and secondary plantlets were regenerated on MMS-medium containing ANK or MS-medium without any growth regulators which in turn grew into bulblets suitable for splitting within 12–16 weeks. A total of 700–1000 bulblets could be obtained from each initial bulb within 12 months. Anatomical studies showed that the shoots were initiated from the epidermis and hypodermis on the abaxial surface of the meristematic tissue of the basal plate of the bulb scale. This technique is useful for the multiplication and preservation of a genotype, since plantlets regenerated in this manner should be genetically uniform.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01983226
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  • 5
    Electronic Resource
    Electronic Resource
    In vitro propagation of Amaryllis belladonna (1992)
    Springer
    Plant cell, tissue and organ culture 31 (1992), S. 179-184 
    Details
    ISSN: 1573-5044
    Keywords: Belladonna lily ; Cape belladonna ; March lily ; micropropagation ; sucrose ; tissue culture ; twin-scales
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Amaryllis belladonna L. plants were multiplied successfully by means of tissue culture techniques. Different plant parts were tested as explant material, but plantlets could only be generated from the twin-scales and immature scapes. These in vitro-formed plantlets were divided into four parts and used for further multiplication. The twin-scale explants had the highest multiplication rate when a medium with 22.2 μM benzyladenine and 0.54 μM naphthaleneacetic acid was used. The sucrose concentration played an important role in the initiation of new plantlets, and the best results were obtained when a sucrose concentration of 2–3% was used. Anatomical observations were made during the initiation of the new plantlets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036221
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