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  • wheat  (112)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 111 (1990), S. 181-189 
    ISSN: 1573-0832
    Keywords: mycotoxin ; ochratoxin ; Penicillium ; storage ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Eleven-kilogram parcels of HY-320 wheat, a cultivar of the new Canada Prairie Spring class, were kept at 15 and 19% initial moisture contents (IMC) in simulated storage in a Manitoba farm granary for 60 weeks to determine biotic and abiotic changes and mycotoxin production. Ochratoxin A reached a maximum of 0.24 ppm by week 20 in the 19% IMC wheat, but was absent in the 15% IMC wheat; no other mycotoxins were detected. Temperature, moisture content, O2 and CO2 levels, fat acidity values, seed germination, microfloral incidence and abundance, and the presence of other mycotoxins were monitored. Principal component analysis of all variables showed that the first principal components accounted for 32–41% of the system variability, and contained the ochratoxin A variable. Ochratoxin A was produced in moist grain that had decreased seed germination andAltermaria activity, and high fungal activity byPenicillium andAspergillus versicolor. Compared to other stored cereals previously studied, HY-320 wheat would be ranked in a low-risk category for mycotoxin formation, based on the ochratoxin A levels observed.
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  • 2
    ISSN: 1573-0832
    Keywords: Fusarium ; mycotoxins ; occurrence ; trichothecenes ; wheat ; zearalenone ; deoxynivalenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Wheat for human consumption (140 samples) was collected after harvest from all regions of Bulgaria. The 1995 crop year was characterized by heavy rainfall in the spring and summer months. The internal mycoflora of wheat samples was dominated by Fusarium spp. and Alternaria spp., and storage fungi were rarely present. The samples were analysed for contamination with Fusarium mycotoxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), T-2 Toxin (T-2), diacetoxyscirpenol (DAS), and zearalenone (ZEA), using enzyme immunoassay methods. DON and ZEA were the predominant toxins, with a contamination frequency of 67% and 69%, respectively. The average levels of these toxins in positive samples were 180 μg/kg (DON) and 17 μg/kg (ZEA), maximum concentrations were 1800 μg kg−1 and 120 μg kg−1, respectively. Acetyl derivatives of DON, namely 3-AcDON and 15-AcDON, were found in 2.1 % and 0.7% of the samples, at at maximum level of about 100 μg kg−1. Only one sample was positive for T-2 (55 μg/kg), DAS was not detected. This is the first report about the natural occurrence of a range of Fusarium mycotoxins in wheat for human consumption in Bulgaria.
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  • 3
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    Springer
    Nutrient cycling in agroecosystems 46 (1996), S. 225-234 
    ISSN: 1573-0867
    Keywords: long-term experiment ; maize ; wheat ; fertilizers ; farm yard manure ; weedicide application ; yield sustainability ; zinc deficiency ; nutrient uptake ; cropping sequence ; organic carbon build-up
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Long-term field experiments play an important role in understanding the complex interactions of plants, soils, climate and management and their effects on sustainable crop production. A long-term fertilizer experiment with maize-wheat-cowpea (fodder) is in progress since 1971 at Punjab Agricultural University farm Ludhiana, India. The experimental result for the first 21 years showed that application of N alone or in combination with P did not produce as much maize and wheat grains as the application of N, P and K together. Eight years after the start of the experiment, the optimal levels of N, P and K application (100% NPK) were unable to sustain the similar (maize) yield level as before because of Zn deficiency. Whereas in FYM amended plots the Zn deficiency did not appear and the higher crop yields could be sustained. The chemical control of weeds could not sustain the maize productivity at the same level as the manual removal of weeds. It was concluded that the high level of crop production can be sustained with the application of N, P and K under intensive cropping system provided deficiency of any of the micronutrient does not crop up. The deficiency of Zn is most likely to occur in semi-arid light textured alluvial soils under intensive cropping without the addition of farm yard manure/organic manures. In maize based cropping systems, manual control of weeds may be preferred to the chemical one. Addition of FYM in conjunction with 100% NPK is most beneficial both from bio-physical and economic point of view.
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  • 4
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    Nutrient cycling in agroecosystems 21 (1990), S. 163-166 
    ISSN: 1573-0867
    Keywords: Critical level of Zn ; alkaline soils ; Zn-deficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field experiments were conducted at 32 locations, chosen for their wide range in DTPA extractable Zn, to determine the critical deficiency level of Zn for predicting response of wheat to Zn application. Soil application of 5.6 kg Zn ha−1 significantly increased the grain yield in deficient soils. Soil extractable Zn was significantly related with per cent grain response and absolute grain yield. Both the graphical and statistical methods of Cate and Nelson indicated the critical level to be 0.75 mg kg−1 soil of DTPA extractable Zn. This level gave a predictability value of 82 per cent.
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  • 5
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    Nutrient cycling in agroecosystems 22 (1990), S. 147-159 
    ISSN: 1573-0867
    Keywords: Sulfate ; elemental sulfur ; leaching ; ryegrass ; wheat ; greenhouse experiments ; split application
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three factorial experiments with four replications were conducted in a greenhouse to examine the effectiveness of gypsum, elemental sulfur (ES powder) and three S containing N fertilizers, viz., ammonium sulfate (AS), urea + ES, and Ureas (20% AS and 80% urea). All experiments were conducted twice in different years. In the first experiment with uncropped soil, the effects of soil type, leaching rate (2.3 and 6.9 mm water per day) and urea addition on sulfate leaching losses were studied. Leaching losses decreased in the order Ureas 〉 ammonium sulfate (AS) 〉 gypsum ≫ urea + ES. Increasing the leaching rate greatly increased sulfate losses from both soils. Losses were greater in the sandy Typic Hapludoll than in the clayey Oxic Paleustalf. Sulfate adsorption was found to decrease strongly with rising the pH in both soils. Hydrolysis of urea temporarily raised the pH of the soil, thereby increasing the sulfate leaching losses. In the second experiment the effects of S rate (0–65 mg per kg soil), split application and leaching rate (0 and 2.3 mm per day) on sulfate leaching losses and ‘apparent S recovery’ (ASR) by three successive cuts of ryegrass (Lolium perenne L.) were studied. Herbage yield more than doubled when S was applied. The effectiveness of the sulfate fertilizers was greater when S was split-applied than given all at once. With split applications the ASR decreased in the order: Ureas 〉 AS 〉 gypsum 〉 urea + ES 〉 ES powder. ES fertilizers were least effective, because the oxidation rate of ES to sulfate was clearly too slow. In the third experiment the effects of S rate (0–40 mg per kg soil) and split application on sulfate leaching losses and ASR in the grain of wheat (Triticum aestivum L.) were studied under leaching conditions (2.3 mm per day). Grain yield increased strongly due to S application. Split application greatly increased the effectiveness of the sulfate fertilizers and appeared to be an effective tool in satisfying the S need of the crop under leaching conditions. Again, ES fertilizers were least effective, because the oxidation rate of ES was too slow to meet the S demand of the crop. In all experiments leaching losses of sulfate from the ES fertilizers were smaller than from the sulfate fertilizers.
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  • 6
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    Nutrient cycling in agroecosystems 22 (1990), S. 97-107 
    ISSN: 1573-0867
    Keywords: Superphosphate ; placement depth ; banding ; relative effectivenesss ; lupins ; wheat ; field experiment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a field experiment on a sandplain soil in a low rainfall (326mm per annum) Mediterranean environment of south-western Australia, seven levels of single superphosphate, 0, 7.5, 10, 14, 19.5, 30 and 39 kg P ha−1, were placed at either 3, 5, 7, 9, 11 or 13 cm depth before sowing wheat (Triticum aestivum) at 3 cm. In a separate treatment, superphosphate was drilled with the seed (the normal practice). In the second year, the plots were sown with lupins (Lupinus angustifolius) at 3 cm depth with no additional superphosphate. In three separate treatments, superphosphate at 0, 14 and 39 kg P ha−1, was drilled with the lupin seed (the normal practice) on plots that had received no superphosphate in the first year. Yields of wheat and lupins were used as a measure of the effectiveness of the superphosphate placement treatments relative to the effectiveness of superphosphate drilled with seed of wheat (year 1) or lupins (year 2), to give relative effectiveness (RE) values in each of the two years. In the first year the RE of superphosphate was increased by about 20% when the fertilizer was placed 5 to 9 cm deep in the soil. In the second year, the RE of superphosphate for producing lupin grain was increased by about 30–60% where the fertilizer had been placed 5–13 cm deep in the previous year compared with freshly drilled 3 cm deep. The yield of wheat or lupins was closely related to the P content of plant tissue; each relationship was independent of the depth or year of superphosphate application.
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  • 7
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    Springer
    Nutrient cycling in agroecosystems 26 (1990), S. 229-235 
    ISSN: 1573-0867
    Keywords: Grass ; wheat ; nitrogen nutrition ; dilution curve ; mineral content ; mineral removal ; phosphorus ; potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The important effect of nitrogen in changing the patterns of mineral content and mineral removal is analysed for grass swards and wheat. Different models are proposed; accumulated dry matter developed throughout a growing period is shown to be an excellent reference for assessing the evolution of the plant mineral content and the mineral removal the growing crop. Applications in diagnosing mineral nutrition status and optimising fertilizer use are proposed and discussed.
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  • 8
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    Cellular and molecular life sciences 46 (1990), S. 1016-1017 
    ISSN: 1420-9071
    Keywords: In vitro absorption ; calcium ; wheat ; Bengal gram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The in vitro absorption of calcium from the duodenum was significantly less in a group of rats fed on a wheat diet than in a group fed a wheat and Bengal gram (70∶30) diet.
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  • 9
    ISSN: 1573-1561
    Keywords: Allelochemicals ; no-tillage ; conventional-tillage ; soils ; wheat ; Triticum aestivum ; mass spectrometry ; Petri-dish bioassay ; fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Putative allelochemicals found in the soil of no-tillage and conventional-tillage wheat plots near Stillwater, Oklahoma, were obtained by a mild alkaline aqueous extraction procedure, bioassayed to determine their biological activity, purified, and analyzed with a capillary gas chromatography-mass spectrometry-data analysis system. The most significant inhibition was found in bioassays of extracts from soil collected immediately after harvest in June, July, and August. No-tillage soils produced significant inhibition during the rest of the year also. Mass spectrometry showed fatty acids as the most abundant compounds. However, when bioassayed authentic samples of the five free fatty acids showed no significant biological activity toward wheat.
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  • 10
    ISSN: 1573-5028
    Keywords: tRNA-like sequences ; t-elements ; RNA processing ; mitochondria ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have recently described the properties of a wheat mitochondrial extract that is able to process, accurately and efficiently, artificial transcripts containing wheat mitochondrial tRNA sequences, with the production of mature tRNAs (P.J. Hanic-Joyce and M.W. Gray, J. Biol. Chem., in press). Such processing involves 5′-endonucleolytic, 3′-endonucleolytic, and TRNA nucleotidyltransferase activities. Here we show that this system also acts on transcripts containing sequences corresponding to an unusual class of short repeats (‘t-elements’) in wheat mtDNA. These repeats are theoretically capable of assuming a tRNA-like secondary structure, although stable transcripts corresponding to them are not detectable in vivo. We find that t-element sequences are processed with the same specificity and with comparable efficiency as are authentic tRNA sequences. Because known t-elements are located close to and in the same transcriptional orientation as active genes (18S-5S, 26S, tRNAPro) in wheat mtDNA, our results raise the question of whether t-elements play a role in gene expression in wheat mitochondria.
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  • 11
    ISSN: 1573-5028
    Keywords: gene expression ; photosynthesis ; protein turnover ; psbA ; tac promoter ; D1 protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Over-expression of the psbAIII gene encoding for the D1 protein (form II; D1:2) of the photosystem II reaction centre in the Synechococcus sp. PCC 7942 was studied using a tac promoter and the lacI Q system. Over-expression was induced with 40 μg/ml IPTG in the growth medium for either 6 or 12 h at growth irradiance (50 μmol photons m-2 s-1). This treatment doubled the amount of psbAII/III mRNA and the D1:2 protein in membranes but decreased the amount of psbAI messages and the D1:1 protein. The total amount of both heterodimeric reaction centre proteins, D1 and D2, remained constant under growth light conditions, indicating that the number of PSII centres in the membranes was not affected, only the form of the D1 protein was changed from D1:1 to D1:2 in most centres. When the cells were photoinhibited either at 500 or 1000 μmol photons m-2 s-1, in the presence or absence of the protein synthesis inhibitor lincomycin, the D1:2 protein remained at a higher level in cells in which over-expression had been induced by IPTG. These cells were also less prone to photoinhibition of PSII. It is suggested that the tolerance of cells to photoinhibition increases when most PSII reaction centres contain the D1:2 protein at the beginning of high irradiance. This tolerance is further strengthened by maintaining psbAIII gene over-expression during the photoinhibitory treatment.
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  • 12
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    Plant molecular biology 30 (1996), S. 1301-1306 
    ISSN: 1573-5028
    Keywords: abscisic acid ; glycine-rich ; ribonucleoprotein ; RNA-binding protein ; RNP motif ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings. whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene.
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  • 13
    ISSN: 1573-5028
    Keywords: differential display ; heat shock proteins ; non-coding regions ; PCR ; reverse transcription ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific probes from 3′ non-coding regions of different members can assist in the fast retrieval and characterization of individual members of a multigene family. We used the recent technique of differential display for the same purpose. As an example of a multigene family in plants, we selected a heat shock protein gene family, HSP16.9 from wheat, with estimated 12 members. We modified the original differential display technique for selective amplification of the 3′ non-coding regions of different wheat HSP16.9 genes by replacing the random 10-mer in the original method with a conserved HSP16.9 gene family-specific primer. Sixteen cDNA fragments from these experiments were sequenced and they represent 8 different members of a 12 member gene family. Our succes can be attributed to shorter 3′ non-coding regions that are typical of higher-plant genes and use of highly conserved gene family-specific primer in these experiments. This modified differential display technique can be of general application to other plant systems where cloning of the different members of a gene family is desired.
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  • 14
    ISSN: 1573-5028
    Keywords: male sterility ; ovary ; parthenogenetic lines ; pollen allergen ; subtractive hybridization ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To isolate genes specifically expressed at the initiation of plant embryo development we have applied a sensitive subtractive hybridization technique for three isogenic wheat lines of the so-called ‘Salmon system’ with either zygotic or autonomous embryo development. Here we present a gene sequence showing a high homology to grass pollen allergens of type II/III thought to be expressed in pollen tissue only. Surprisingly, the pollen allergen-like sequence, designated Tri a III, is also expressed in gynoecia of the sexual, male fertile wheat line ‘(aestivum)-Salmon’, whereas the two parthenogenetic and male sterile wheat lines ‘(caudata)-Salmon’ and ‘(kotschyi)-Salmon’ completely lack any Tri a III transcript. Our data suggest a positive correlation between the expression of this clone and the manifestation of male fertility. Northern and in situ hybridization analysis revealed that, in addition to its presence in pollen, Tri a III is expressed in the parenchymatous tissue of ‘(aestivum)-Salmon’ ovaries exclusively at the day of anthesis. This precise temporal and spatial expression pattern suggests a more general function of the pollen allergen-like sequence Tri a III not limited to the exhibition of allergens in pollen grains.
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  • 15
    ISSN: 1573-5028
    Keywords: antisense ; Corynebacterium glutamicum ; Escherichia coli ; Flaveria trinervia ; overexpression ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phosphoenolpyruvate carboxylase (PEPC) genes from Corynebacterium glutamicum (cppc), Escherichia coli (eppc) or Flaveria trinervia (fppc) were transferred to Solanum tuberosum. Plant regenerants producing foreign PEPC were identified by Western blot analysis. Maximum PEPC activities measured in eppc and fppc plants grown in the greenhouse were doubled compared to control plants. For cppc a transgenic plant line could be selected which exhibited a fourfold increase in PEPC activity. In the presence of acetyl-CoA, a known activator of the procaryotic PEPC, a sixfold higher activity level was observed. In cppc plants grown in axenic culture PEPC activities were even higher. There was a 6-fold or 12-fold increase in the PEPC activities compared to the controls measured in the absence or presence of acetyl-CoA, respectively. Comparable results were obtained by transient expression in Nicotiana tabacum protoplasts. PEPC of C. glutamicum (PEPC C.g.) in S. tuberosum leaf extracts displays its characteristic K m(PEP) value. Plant growth was examined with plants showing high expression of PEPC and, moreover, with a plant cell line expressing and antisense S. tuberosum (anti-sppc) gene. In axenic culture the growth rate of a cppc plant cell line was appreciably diminished, whereas growth rates of an anti-sppc line were similar or slightly higher than in controls. Malate levels were increased in cppc plants and decreased in antisense plants. There were no significant differences in photosynthetic electron transport or steady state CO2 assimilation between control plants and transformants overexpressing PEPC C.g. or anti-sppc plants. However, a prolonged dark treatment resulted in a delayed induction of photosynthetic electron transport in plants with less PEPC. Rates of CO2 release in the dark determined after a 45 min illumination period at a high proton flux density were considerably enhanced in cppc plants and slightly diminished in anti-sppc plants. When CO2 assimilation rates were corrected for estimated rates of mitochondrial respiration in the light, the electron requirement for CO2 assimilation determined in low CO2 was slightly lower in transformants with higher PEPC, whereas transformants with decreased PEPC exhibited an appreciably elevated electron requirement. The CO2 compensation point remained unchanged in plants (cppc) with high PEPC activity, but might be increased in an antisense plant cell line. Stomatal opening was delayed in antisense plants, but was accelerated in plants overexpressing PEPC C.g. compared to the controls.
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  • 16
    ISSN: 1573-5028
    Keywords: α-amylase tetrameric inhibitor ; cDNA cloning ; genetic mapping ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have characterized three cDNA clones corresponding to proteins CM1, CM3 and CM16, which represent the three types of subunits of the wheat tetrameric inhibitor of insect α-amylases. The deduced amino acid sequences of the mature polypeptides are homologous to those of the dimeric and monomeric α-amylase inhibitors and of the trypsin inhibitors. The mature polypeptides are preceded by typical signal peptides. Southern blot analysis of appropriate aneuploids, using the cloned cDNAs as probes, has revealed the location of genes for subunits of the CM3 and of the CM16 type within a few kb of each other in chromosomes 4A, 4B and 4D, and those for the CM1 type of subunit in chromosomes 7A, 7B and 7D. Known subunits of the tetrameric inhibitor corresponding to genes from the B and D genomes have been previously characterized. No proteins of this class have been found to be encoded by the A genome in hexaploid wheat (genomes AA, BB, DD) or in diploid wheats (AA) and no anti α-amylase activity has been detected in the latter, so that the A-genome genes must be either silent (pseudogenes) or expressed at a much lower level.
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  • 17
    ISSN: 1573-5028
    Keywords: cDNA ; expression ; FKBP ; PPIase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel cDNA encoding for a peptidyl-prolyl-cis-trans-isomerase (PPIase) belonging to the FK506-binding protein (FKBP) family was isolated from wheat. It contains an open reading frame of 559 amino acids and it represents the first plant FKBP-PPIase to be cloned. It possesses a unique sequence which is composed of three FKPB-like domains, in addition to a putative tetratricopeptide repeat (TPR) motif and a calmodulin-binding site. The recombinant FKBP-PPIase expressed in and purified from Escherichia coli exhibits PPIase activity that is efficiently inhibited by the immunosuppressive drugs FK506 and rapamycin. Northern blot analysis showed that wheat FKBP was found mainly in young tissues. Polyclonal antibodies revealed the presence of cross-reacting proteins in embryos, roots and shoots. The unique structural features, the enzymatic activity and the presence of putative isoforms in wheat tissues indicate the possibility of the involvement of wheat PPIase in essential biological functions, similar to other members of the FKBP gene family.
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  • 18
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    Plant molecular biology 15 (1990), S. 793-795 
    ISSN: 1573-5028
    Keywords: mitochondrial gene ; ORF25 ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The wheat mitochondrial orf25 nucleotide sequence of 576 pb has been determined. Its derived protein sequence shares 88% and 75% amino acid identity with those of maize and tobacco mitochondria, respectively. The wheat and tobacco orf25 sequences lack four inserts, of 6 bp to 36 bp, that are present in the maize homologue. The wheat orf25 gene is actively transcribed and is preceded by a regulatory sequence block very similar to those located upstream of the wheat coxII and atp6 genes. Our observations support the view that orf25 sequences encode a functional polypeptide in plant mitochondria.
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  • 19
    ISSN: 1573-5028
    Keywords: Cyanobacteria ; photosynthesis ; random mutagenesis ; sodium bisulfite ; thylakoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To identify amino acid residues of the D2 protein that are critical for functional photosystem II (PS II), sodium bisulfite was utilized for in vitro random mutagenesis of the psbDI gene from Synechocystis sp. PCC 6803. Sodium bisulfite reacts specifically with cytosine in single-stranded regions of DNA and does not attack double-stranded DNA. Using a hybrid plasmid that was single-stranded in the region to be mutagenized and that was double-stranded elsewhere, mutations were targeted to a specific psbDI region coding for the lumenal A-B loop of the D2 protein. Several mutants were isolated with a total of 15 different amino acid changes in the loop. The majority of these mutations did not result in a loss of photoautotrophic growth or in significantly altered PS II function. However, mutation of Glu-69 to Lys, Ser-79 to Phe, and Ser-88 to Phe were found to influence photosystem II activity; the importance of the latter two residues for proper PS II function was unexpected. Cells carrying the double mutation S79F/S88F in D2 did not grow photoautotrophically and had no functionally active PS II centers. The single mutant S79F was also incapable of photoautrophic growth, but displayed reasonably stable oxygen evolution, while PS II function in the single mutant S88F appeared to be close to normal. Because of the more pronounced phenotype of the S79F/S88F strain as compared to the single mutants, both Ser residues appear to affect stable assembly and function of the PS II complex. The mechanism by which the S79F mutant loses photoautotrophic growth remains to be established. However, these results show the potential of targeted random mutagenesis to identify functionally important residues in selected regions of proteins.
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  • 20
    ISSN: 1432-203X
    Keywords: Triticum aestivum L. ; wheat ; transformation ; biolistics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed a method for the accelerated production of fertile transgenic wheat (Triticum aestivum L.) that yields rooted plants ready for transfer to soil in 8–9 weeks (56–66 days) after the initiation of cultures. This was made possible by improvements in the procedures used for culture, bombardment, and selection. Cultured immature embryos were given a 4–6 h pre-and 16 h post-bombardment osmotic treatment. The most consistent and satisfactory results were obtained with 30 μg of gold particles/bombardment. No clear correlation was found between the frequencies of transient expression and stable transformation. The highest rates of regeneration and transformation were obtained when callus formation after bombardment was limited to two weeks in the dark, with or without selection, followed by selection during regeneration under light. Selection with bialaphos, and not phosphinothricin, yielded more vigorously growing transformed plantlets. The elongation of dark green plantlets in the presence of 4–5 mg/l bialaphos was found to be reliable for identifying transformed plants. Eighty independent transgenic wheat lines were produced in this study. Under optimum conditions, 32 transformed wheat plants were obtained from 2100 immature embryos in 56–66 days, making it possible to obtain R3 homozygous plants in less than a year.
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  • 21
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    Theoretical and applied genetics 79 (1990), S. 305-313 
    ISSN: 1432-2242
    Keywords: Peroxidase ; Isoelectric focusing ; Hexaploid ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Isoelectric focusing (IEF) of extracts from different tissues of hexaploid wheat cv “Chinese Spring” provided a method of distinguishing and identifying the four known, and one newly discovered, sets of genes encoding peroxidase isozyme production.Per-1, carried on the short arms of homoeologous group 1 chromosomes, shows a high degree of conservation and is active in coleoptile tissue.Per-2, carried on the short arms of group 2 chromosomes, shows some polymorphism and is most active in root tissue.Per-3, on the long arms of group 3 chromosomes, is highly variable and most active in embryo tissue.Per-4, carried on chromosome arms7AS,4AL, and7DS, is quite variable and most active in endosperm tissue. (The chromosome nomenclature used in this paper is that agreed to by the 7th International Wheat Genetics Symposium, where the previous designations of4A and4B were reversed.) Restriction fragment length polymorphism (RFLP)-based maps of the group 7 chromosomes were used to locatePer-A4 to a distal region of7AS. In addition, a further set of genes was identified as being active in root tissue. In wheat a single locus,Per-D5, was found on chromosome arm2DS.
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  • 22
    ISSN: 1610-7403
    Keywords: Chamaecyparis obtusa Endl. ; photosynthesis ; Pinus densiflora Sieb. et Zucc. ; stomatal conductance ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was conducted to investigate the potential for modifying drought tolerance of Japanese cypress (Chamaecyparis obtusa Endl.) and Japanese red pine (Pinus densiflora Sieb. et Zucc.). Three-year-old seedlings were controlled for five-months at three different soil water potentials ({ie73-1}). Japanese cypress exposed to high {ie73-2} was able to maintain higher photosynthesis (Phn), transpiration (Tr) and stomatal conductance to H2O (gH2O) in comparison to low {ie73-3} pretreatments, however, there was no significant difference in Phn for Japanese red pine. Soil water potential at the threshold from the maximum to limited Phn was higher in high {ie73-4} pretreatments than in low {ie73-5} pretreatments. Net photosynthesis, Tr and gH2O decreased more rapidly in high {ie73-6} pretreatments than in low {ie73-7} pretreatments. Transpiration decreased more significantly than Phn, thus, resulted in increased water use efficiency. All these factors are likely to result in significant improvements in the drought tolerance. Japanese red pine seems more drought-tolerant than Japanese cypress. Japanese cypress is suitable to soil of −0.05 MPa water potential, and Japanese red pine is suitable to −0.16 MPa and even dryer soils.
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  • 23
    ISSN: 1610-7403
    Keywords: chlorophyll fluorescence ; electron transport ; photorespiration ; photosynthesis ; quantum yield ; tree
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using attached and detached leaves ofAcer palmatum Thunb. andRhaphiolepsis umbellata Makino, pulse-modulated chlorophyll fluorescence and CO2 exchange were measured. Quantum yield of photosynthesis was determined from the fluorescence parameter(Fm′−Fs)/Fm′, where (Fm′−Fs) was defined as the difference between steady state chlorophyll fluorescence (Fs) and maximum fluorescence (Fm′) elicited by a saturating light pulse. The rate of electron transport through photosystem II (total electron flow) was calculated from the product of quantum yield andA (PFD), whereA is the rate of absorbed photons as given by leaf absorptance, and PFD is the photon flux density at the leaf surface. The rate of electron transport dependant on CO2 uptake (assimilative electron flow) was calculated from the gross photosynthetic rate in a leaf. The difference between the rates of total and assimilative electron transport was denoted as the rate of non-assimilative electron transport which depends on photorespiration and oxygen reduction. Available data provided quantitative information on the rate of non-assimilative electron flow in intact leaves. When leaf photosynthesis ofA. palmatum was measured under sunlight, the rates of total and assimilative electron transport were determined to be approximately 900 and 150 μmol equiv. e/mg Chl·h, respectively. The difference (750 μmol equiv. e/mg Chl·h) was attributed to the activity of non-assimilative electron flow. The ratio of total to assimilative electron flow was found to increase gradually with rising in irradiance. The results suggest that non-assimilative electron flow occurred at much higher rate than assimilative electron flow at high irradiance. Implications of the results are briefly discussed in relation to photosynthesis limitation in tree leaves.
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  • 24
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    Journal of applied phycology 8 (1996), S. 263-273 
    ISSN: 1573-5176
    Keywords: molecular biology ; mutagenesis ; photosynthesis ; protein engineering ; respiration ; thylakoid membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cyanobacteriumSynechocystis sp. PCC 6803 is readily amenable to targeted mutagenesis: Foreign DNA is taken up spontaneously, and after uptake DNA can be integrated into the organism's genome by homologous recombination. Using appropriate DNA constructs for transformation, specific genes in the organism can be interrupted, deleted, or replaced by modified gene copies. The organism can grow under a number of different conditions, ranging from photoautotrophic to fully heterotrophic modes, making genetic modifications that alter fundamental processes such as photosynthesis and/or respiration feasible. For example, deletion of photosystem I leads to an obligate (photo)heterotrophic strain in which photosystem II-generated electrons appear to be consumed by respiratory processes, whereas deletion of photosystem II leads to an obligate (photo)heterotrophic strain in which cyclic electron flow around photosystem I appears to remain active. A major advantage ofSynechocystis sp. PCC 6803 is that its entire genome has been sequenced (by S. Tabata and co-workers), opening many avenues to address basic and applied research problems. For example, genes can be introduced, modified or deleted, and hypotheses regarding the function of an open reading frame can be tested by deletion of this open reading frame. Methods to modify genes are numerous. In addition to site-directed mutagenesis, novel molecular genetic approaches including ‘targeted random mutagenesis’, combinatorial mutagenesis and introduction of hybrid genes have come of age and have proven to be very powerful tools in protein engineering. These approaches have been utilized primarily in this strain to study photosynthesis, but applications of this technology, including pathway engineering, alterations of substrate specificity of enzymes and introduction of tolerance to a variety of stresses, are equally feasible in relation to more applied aims. For optimal utilization of the potential of theSynechocystis sp. PCC 6803 system, however, an increased emphasis toward understanding the biochemistry and molecular physiology of cyanobacteria will also be critically important.
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  • 25
    ISSN: 1573-5176
    Keywords: fluorescence ; photoinhibition ; photosynthesis ; Spirulina ; photobioreactor ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A chlorophyll fluorescence technique was applied to anin situ study on the effects of low temperature and high light stresses onSpirulina cultures grown outdoors in controlled tubular photobioreactors at high (1.1 g L−1) and low (0.44 g L−1) biomass concentrations. Diurnal changes in PSII photochemistry (F v/F m) after 15 min of darkness, or in the light (dF/F′ m), and non-photochemical (qN) quenching were measured using a portable, pulse-amplitude-modulated fluorometer. The depression of theF v/F m ratio ofSpirulina cultures grown outdoors at 25°C (i.e. 10°C below optimum for growth) and 0.44 g L−1, reached 30% at the middle of the day. At the same time of the day thedF/F′ m ratio showed a reduction of up to 52%. The depression of bothF v/F m anddF/F′ m was lower in the cultures grown at 1.1 g L−1. Photoinhibition reduced the daily productivity of the culture grown at 0.44 g L−1 and 25°C by 33% with respect to that grown at 35°C. Changes in the growth yields of the cultures grown under different temperatures and growth rates correlate well with analogous changes in photon yield (dF/F′ m). Simple measurements of photochemical yield (F v/F m) can be used to test the physiological status ofSpirulina cultures. The results indicate that the saturating pulse fluorescence technique, when usedin situ, is a powerful tool for assessment of the photosynthetic characteristics of outdoor cultures ofSpirulina.
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  • 26
    ISSN: 1573-5176
    Keywords: herbicide ; DCMU ; photosynthesis ; monoalgal culture ; Chaetoceros ; Dunaliella ; Nannochloropsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The selective effect of DCMU on photosynthetic activity and growth rate was examined in several marine unicellular algae:Nannochloropsis sp. (Eustigmatohyceae),Dunaliella salina (Chlorophyceae)Isochrysis galbana (Prymnesiophyceae) andChaetoceros sp. (Bacillariophyceae). DCMU at 10−7 M caused an immediate decrease in the photosynthetic rate ofDunaliella andIsochrysis (about 50% inhibition), whereas 10−6 M imposed 80% inhibition in the photosynthetic rate ofChaetoceros. InNannochloropsis the rate was affected only when DCMU concentration exceeded 10−6M. Cellular growth rate of all studied algae was affected by DCMU in a similar manner to photosynthesis. The differential effect of DCMU was further examined in mixed cultures in whichNannochloropsis was cultivated together with an additional species simulating a contamination situation of aNannochloropsis culture. When DCMU was added at concentrations higher than 10−7 M, the growth of the competing algae significantly decreased, whileNannochloropsis maintained a relatively high growth rate. Consequently, after a growth period of 4 to 7 days a clear domination ofNannochloropsis was observed. These results demonstrate that DCMU and probably other herbicides of similar characteristics can be used effectively as a selective tool to suppress contaminating unicellular algae in open ponds in order to maintain a monoculture ofNannochloropsis.
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  • 27
    ISSN: 1573-5176
    Keywords: Spirulina platensis ; cyanobacterium ; NaCl ; photoinhibition ; photosynthesis ; photosystem II ; salt stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response ofSpirulina platensis cells to salinity stress was studied. Once adapted to the higher osmoticum, photosynthetic parameters such as the maximum rate of photosynthesis under saturating irradiance (Pmax) and the initial slope of the P-I curve (α) are reduced by 15% and 25% in 0.5 M NaCl grown cells, respectively. Salt-adapted cells have a modified biochemical composition; reduced protein and chlorophyll content, and an increased level of carbohydrates. The reduction in the photosynthetic capacity of the salt-adaptedSpirulina cells reflects a lower ability to utilize light energy and results in an increase in the susceptibility of the stressed cells to photoinhibition. This conclusion is supported by the finding that cultures exposed to salt stress show not only a decrease in growth rate (μ), but lose the ability to respond to increased irradiance with an increase in growth. The use of variable fluorescence as a fast and reliable measurement to follow the changes in PSII of salt-stressesSpirulina cells enables following the early events of salinity shock. It indicates that as soon as the cells are exposed to salt, a protection mechanism is induced. This mechanism does not require any protein synthesis and may take place even in the dark, though at somewhat reduced effectiveness. The significance of the result in providing a better understanding of the interaction between two environmental stresses — light and salinity — and their application in the outdoor mass cultivation ofSpirulina are discussed.
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  • 28
    ISSN: 1618-2545
    Keywords: consecutive planting ; Phoma sp. ; plant growth-promotion ; potting medium ; soybean ; wheat ; zoysiagrass rhizosphere fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several isolates ofPhoma sp., certain nonsporulating fungi, as well asPenicillium andTrichoderma, all isolated from zoysiagrass rhizosphere, promoted growth of wheat and soybean under greenhouse conditions. However, the ability of these rhizosphere fungi to enhance plant growth varied with the crop tested. For example, most of the fungi effectively promoted the growth of wheat, whereas only a few fungi were effective on soybean. In consecutive plantings of wheat and soybean grown in soil previously infested with these zoysiagrass rhizosphere fungi, the growth promotion ability of the fungi was lowered. However, addition of fresh potting medium appeared to restore their growth-promotive effects. It appears that the activation of plant growth-promoting fungi in soil might depend on the availability of organic substrates to colonize, as evidenced by the promotion of plant growth.
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  • 29
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    Journal of applied phycology 2 (1990), S. 293-296 
    ISSN: 1573-5176
    Keywords: heavy metal ; photosynthesis ; periphyton ; tolerance ; Cyanophyceae ; genetic adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study was made of the tolerance to Cu of 11 strains of Cyanophyceae and 7 strains of eukaryotes. These had all been tested within 6 months after isolation for their photosynthetic activity when exposed to Cu (Takamuraet al., 1989) and had repeatedly been subcultured in the medium without Cu for 2 years. Photosynthetic measurements were made in two ways: precultured in medium without Cu or precultured (for one subculture) in medium containing Cu (645 μg 1−1). The results were compared with those obtained within 6 months of isolation. The tolerance of the eukaryotes did not change significantly in any case, but most strains of Cyanophyceae lost their tolerance to Cu within a few subcultures in medium without Cu; however tolerance recovered following one subculture in medium containing an intermediate level of Cu. This rapid adaptation cannot be explained by a constitutive mutation.
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  • 30
    ISSN: 1573-5176
    Keywords: Cystoseira barbata ; photosynthesis ; light ; temperature ; salinity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The net photosynthesis of the Mediterranean brown seaweedCystoseira barbata f.repens is measured according to irradiance, temperature and salinity. There is not only, a good utilization of low light intensities (light-shade adaptation), but also a specific ability to use a broad range of irradiance, which corresponds in the photosynthesis-irradiance curves to a high initial slope and an extended light saturation level from 300 to 1500 μmol photon m−2 s−1; only very high irradiances induce photoinhibition. Maximum net photosynthesis occurred at temperatures ranging from 20 °C to 30 °C. The alga tolerates not only a low level of salinity, but also a slight increase in salinity; however, at more than 47.5 g 1−1 NaCl, oxygen exchange is significantly reduced. Light, temperature and salinity requirements are discussed, taking into account ecological considerations. Yields and quality of alginic acid are presented according to the irradiance and yearly evolutionin situ in order to aid future cultivation of this species.
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  • 31
    ISSN: 1573-5176
    Keywords: cyanobacteria ; light intensity ; photoinhibition ; photosynthesis ; recovery ; Spirulina platensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three isolates ofSpirulina platensis (Norst) Geitler marked BP, P4P and Z19/2 were compared with respect to their response and acclimation capability to high photon flux densities (HPFD). Cultures exposed to HPFD (1500–3500 μmol photon m−2 s−1) exhibited a marked decrease in light-dependent O2 evolution rate. P4P was more sensitive to HPFD than the two other isolates. All three isolates recovered from photoinhibition when placed under low PFD. The BP isolate was able to recover also in the dark but to a lower extent and at a lower rate, while no recovery was observed in the other two isolates under dark conditions. No recovery was observed when protein synthesis was inhibited using chloramphenicol. Cultures grown at 200 μmol photon m−2 s−1 differed from cultures grown at 120 μmol photon m 2 s-1 by their lower maximal photosynthetic rate (P max ) and higher light saturation (I k ) value, while being more resistant to HPFD stress. The ability ofSpirulina isolates to acclimate and withstand HPFD may provide useful information for the selection of strains useful for outdoor mass cultivation.
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  • 32
    ISSN: 1573-5176
    Keywords: Gracilaria ; strain selection ; growth ; photosynthesis ; rubisco ; agar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A strain selection procedure using Gracilaria verrucosa gametophytic sporelings was found to be an efficient tool for the improvement of Gracilaria strains. Two strains, C-2 and A-18, which were isolated and grown clonally, showed higher growth rates under high and low temperature conditions, respectively, than the local Gracilaria conferta. Growth rate, photosynthesis and chlorophyll, which were measured under different temperature and photon flux densities, demonstrated an overall advantage of the selected strains over the wild type strains of both G. verrucosa and G. conferta. Growth rates were also generally in positive correlation with the carboxylase activity of Rubisco. The G. verrucosa wild type also had a 40% higher agar content than G. conferta. The selected strains thus showed higher potential for outdoor cultivation than local wild type populations.
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  • 33
    ISSN: 1573-5117
    Keywords: biotest ; brackish ; Gracilaria ; growth inhibition ; marine ; photosynthesis ; Rhodophyta ; seaweed ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A growth inhibition test method was developed using the macroalga Gracilaria tenuistipitata as the test organism. This alga was chosen because of its high laboratory growth rates, commonly 30–40% d−1, which are reached in salinities between 5 and 40‰, and its epiphyte resistance. The toxicity of a number of substances, including heavy metals, herbicides and complex wastewaters towards the alga was assayed. Anti-fouling paints were tested with a modification of the method. EC50 values for heavy metals varied between 0.05 and 17 mg l−1 and for herbicides between 0.002 and 0.02 mg l−l. The sensitivity to the toxicant was generally higher at low salinity. Omitting nitrogen and phosphorus additions to the test medium increased the sensitivity and a semi-static performance was possible with maintained or increased sensitivity. Preliminary tests done with a computerised photosynthesis inhibition method produced promising results. In conclusion, this is a simple, sensitive and reproducible test method for assessing the toxicity of substances, wastewaters and anti-fouling paints in brackish and marine environments.
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  • 34
    ISSN: 1573-6849
    Keywords: in situ hybridization ; intermediate wheatgrass ; translocation ; virus resistance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluorescencein situ hybridization (FISH) was used to determine the breakpoint of the translocation chromosome in two bread wheat (Triticum aestivum) germplasm lines withThinopyrum intermedium chromatin carrying resistance to either wheat streak mosaic virus (WSMV) or barley yellow dwarf virus (BYDV). In addition, genome-specific random amplified polymorphic DNA (RAPD) markers were used to ascertain the genomic sources of theTh. intermedium chromosomes carrying the WSMV or BYDV resistance. CI17766, a WSMV-resistant wheat germplasm line derived from induced homoeologous pairing by using theph1b mutant, had a translocation chromosome composed of the complete 4AL and about 45% of proximal 4AS from wheat, and the entire 4ES ofTh. intermedium. The BYDV-resistant translocation line, TC14, derived from tissue culture, had a very short distal segment of 7StL fromTh. intermedium terminally attached to 56% of the proximal 7DL. These observations indicate that translocations in these wheat germplasm lines did not involve centromeric breaks and fusion but were a result of homoeologous chromosome recombination.
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  • 35
    ISSN: 1573-5036
    Keywords: Lycopersicon esculentum L. ; magnesium-manganese interaction ; magnesium: manganese ratio ; manganese toxicity ; tomato ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Results are reported for tomato (Lycopersicon esculentum L. var. Ailsa craig) and wheat (Triticum aestivum L. cv. Mara) which demonstrate that increasing concentrations of Mg in the plant raises plant tolerance to Mn toxicity. Water culture experiments with tomato show that under conditions of high Mn supply (200 µM, Mn), not only does increasing Mg application (0.75 mM to 15 mM) depress Mn uptake, but the higher Mg concentrations in the shoot counteract the onset of Mn toxicity when the concentrations of Mn in the shoot are also high. The ratio of Mg: Mn in the tissues is a better indicator of the appearance of toxicity symptoms than Mn concentration alone. Toxicity symptoms were observed when the Mg:Mn ratio in the shoot tissue was from 1.13 to a value between 3.53 and 6.54. The corresponding Mg: Mn ratio in the older leaves was from 0.82 to between 2.27 and 3.51. For wheat grown in soil, analyses of leaves revealed that growth could be expressed by the following relationship: Y=A+B exp(-kX), where Y=growth, X=Mg:Mn ratio, A, B and k=constants. Growth was significantly reduced when the Mg:Mn ratio fell below 20:1. From a measurement of this ratio it is therefore possible to predict the appearance of Mn toxicity and its influence on growth.
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  • 36
    ISSN: 1573-5036
    Keywords: calcium ; ear development ; magnesium ; potassium ; tissue press sap ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract For floret development and final grain number ear elongation in wheat (10–17d before anthesis) is the most sensitive period to adverse growth conditions. Sugars are supposedly the main factor determining floret numbers and grain set, and play next to potassium a significant role in establishing turgor pressure in young tissues. In view of this osmotic function, the influence of K on the concentration of water soluble carbohydrates (WSC, total of sucrose and reducing sugars), Ca and Mg was investigated in pot experiments. Further, the osmotic potential of sap extracted from young ears was determined and compared to sap extracted from the leaf blade. Plants supplied with low amounts of K (moderate K-deficiency) had a considerably lower K concentration in the press sap of the flag leaf and the ear than plants well supplied with K. Concentrations of WSC, Ca and Mg were higher in press sap of the flag leaf in K deficient plants than in plants adequately supplied with K. This indicates a substitution of K in its osmotic role. In press sap from ears, however, WSC, Ca and Mg were not influenced by the K application. Therefore, substances other than those measured must have been responsible for the osmoregulation in the young ear. WSC and the osmotic potential increased (more negative) independently on K supply during ear elongation, while K, Ca and Mg concentrations decreased. Whereas grain number was not influenced by the treatment, single grain weight at maturity was reduced by low K availability in the soil.
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  • 37
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    Plant and soil 124 (1990), S. 303-307 
    ISSN: 1573-5036
    Keywords: models ; potassium uptake ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spring wheat was grown in the field under deficient and sufficient levels of soil K and with high and low supplies of fertiliser nitrogen. Measurements were made of K uptake, soil nutrient supply parameters, root growth and, in solution culture, root influx parameters. Mechanistic models predicted uptake reasonably well under K-deficient conditions, but over-predicted uptake, by as much as 4 times, under K-sufficient conditions. The over-prediction was apparently due to poor characterisation of plant demand.
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  • 38
    ISSN: 1573-5036
    Keywords: Cu levels ; N sources ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A pot experiment was conducted, in a greenhouse, at Hisar, India, using a sandy soil deficient in nitrogen and copper, to study the effects of various levels of N and Cu on the dry matter yield and the N and Cu contents of wheat (Triticum aestivum L.). The sources of nitrogen used were Ca(NO3)2, NH4Cl and NH4NO3 applied in amounts necessary to establish 120 ppm of soil nitrogen and using a control (0 ppm N). Copper was applied, as copper chloride, to give soil Cu levels of 0, 5, 10 and 20 ppm. In general, dry matter yields, N and Cu concentrations in shoots and roots and available soil-N after harvest of the plants, followed the order Ca(NO3)〉NH4NO3〉NH4Cl. Up to a level of 5 ppm Cu, the dry matter yields of shoots and roots increased, but decreased at higher levels of Cu. Increasing Cu levels significantly decreased the available soil-nitrogen after harvest and also the concentration of N in the plants. At the same time the concentration of Cu in shoots and roots and available Cu in the soil was increased. Nitrogen and copper were found to have a mutually antagonistic effect on each other's concentration in the plants. The antagonism was greater with NH4 + sources than with NO3 − compounds.
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  • 39
    ISSN: 1573-5079
    Keywords: chlorophyll fluorescence ; energy dissipation ; light scattering ; photosynthesis ; state transition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The light-induced induction of components of non-photochemical quenching of chlorophyll fluorescence which are distinguished by different rates of dark relaxation (qNf, rapidly relaxing and qNs, slowly relaxing or not relaxing at all in the presence brief saturating light pulses which interrupt darkness at low frequencies) was studied in leaves of spinach. After dark adaptation of the leaves, a fast relaxing component developed in low light only after a lag phase. Quenching increased towards a maximum with increasing photon flux density. This ‘fast’ component of quenching was identified as energy-dependent quenching qE. It required formation of an appreciable transthylakoid ΔpH and was insignificant when darkened spinach leaves received 1 s pulses of light every 30 s even though zeaxanthin was formed from violaxanthin under these conditions. Another quenching component termed qNs developed in low light without a lag phase. It was not dependent on a transthylakoid pH gradient, decayed exponentially with a long half time of relaxation and was about 20% of total quenching irrespective of light intensity. When darkened leaves were flashed at frequencies higher than 0.004 Hz with 1 s light pulses, this quenching also appeared. Its extent was very considerable, and it did not require formation of zeaxanthin. Relaxation was accelerated by far-red light, and this acceleration was abolished by NaF. We suggest that qNs is the result of a so-called state transition, in which LHC II moves after its phosphorylation from fluorescent PS II to nonfluorescent PS I. This state transition was capable of decreasing in darkened leaves the potential maximum quantum efficiency of electron flow through Photosystem II by about 20%.
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  • 40
    ISSN: 1573-5079
    Keywords: cyanobacteria ; electron transport ; fluorescence ; photosynthesis ; spillover ; state changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Adaptive responses to excess (supraoptimal) level of cobalt supplied to the growth medium were studied in the cyanobacterium Synechocystis PCC 6803. Growth of cells in the medium containing 10 μM CoCl2 led to a large stimulation (50%) in O2-evolution and an overall increase (∼30%) in the photosynthetic electron transport rates. Analysis of variable Chl a fluorescence yield of PS II and immuno-detection of Photosystem II (PS II) reaction-center protein D1, showed a small increase (15–20%) in the number of PS II units in cobalt-grown cells. Cobalt-grown cells, therefore, had a slightly elevated PS II/PS I ratio compared to control. We observed alteration in the extent of energy distribution between the two photosystems in the eobalt grown cells. Energy was preferentially distributed in favour of PS II accompanied by a reduction in the extent of energy transfer from PS II to PS I in cobalt-grown cells. These cells also showed a smaller PS I absorption cross-section and a smaller size of intersystem electron pool than the control cells. Thus, our results suggest that supplementation of 10 μM CoCl2, to the normal growth medium causes multiple changes involving small increase in PS II to PS I ratio, enhanced funneling of energy to PS II and an increase in PS I electron transport, decrease PS I cross section and reduction in intersystem pool size. The cumulative effects of these alterations cause stimulation in electron transport and O2 evolution.
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  • 41
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    European journal of plant pathology 96 (1990), S. 187-198 
    ISSN: 1573-8469
    Keywords: fusarium head blight ; Fusarium culmorum ; F. graminearum ; wheat ; mycotoxin ; deoxynivalenol ; nivalenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Aaraantasting van tarwe doorFusarium culmorum enFusarium graminearum leidt tot vorming van mycotoxinen in het graan, waarvan deoxynivalenol (DON) en nivalenol (NIV) de belangrijkste toxinen zijn. In dit artikel wordt een overzicht gegeven van de toxicologische aspecten, en het voorkomen van deze toxinen in tarwe. Informatie over DON en NIV in tarwe in West-Europa is schaars. Gebaseerd op gegevens vanFusarium epidemieën in de jaren 1979–1986 wordt een schatting gegeven van de concentratie DON in Nederlandse tarwe. Rekening houdend met de herkomst en verwerking van tarwe, blijken zowel in dierlijk als menselijk voedsel lage concentraties DON chronisch voor te komen. Op basis van een maximaal toelaatbare dagelijkse dosis DON van 3 μg kg−1 lichaamsgewicht is de schatting van de dagelijkse opname van DON in het jaar volgend op de oogst van 1982 net op de grens. Zowel een jaarlijkse inventarisatie vanFusarium aantasting en DON besmetting van het graan, als de ontwikkeling vanFusarium-resistente rassen zijn noodzakelijk.
    Notes: Summary An infection of bread wheat by fusarium head blight contaminates the crop with mycotoxins, particularly deoxynivalenol (DON) and nivalenol (NIV). The toxicity and natural occurrence of these mycotoxins in wheat are reviewed. Based on 8 years data of fusarium head blight epidemics of wheat in the Netherlands, DON contamination of the grain was estimated. Fusarium head blight ratings averaged an infection of 1.7% of all spikelets; estimates for DON contamination averaged 0.9 mg kg−1. Taking a guideline level for DON in uncleaned bread wheat of 2 mg kg−1, in 1979 and 1982 a wheat crop was produced with estimated DON concentrations above the limit of tolerance. Human and animal exposure to mycotoxins in the Netherlands appears to be small but chronic. The information presented in this paper illustrates the need for an annual evaluation of the crop for fusarium head blight incidence and mycotoxin content, and the necessity of fusarium head blight resistant wheat cultivars.
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  • 42
    ISSN: 1573-8469
    Keywords: Canopy enclosure ; stomatal conductance ; light response curve ; light use efficiency ; photosynthesis ; Solanum tuberosum L. ; transpiration ; water use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Aardappelplanten (Solanum tuberosum L.) cv.Saturna werden onderworpen aan stress als gevolg vanVerticillium dahliae en droogte. In vroege stadia van de groei werden stomataire geleiding, transpiratie en netto fotosynthese bij lichtverzadiging (PAR〉300 W m−2) gemeten aan individuele bladeren en met een mobiel instrumentarium met behulp van gewaskappen. Er werden geen significante verschillen gevonden in de waarden van de stomataire geleiding en de gasuitwisslingskarakteristieken als gevolg vanV. dahliae-besmetting tot een maand na opkomst. Daarna leidde infectie metV. dahliae tot een afname van de stomataire geleiding, transpiratie en netto fotosynthese, speciaal bij oudere bladeren en bij planten die meer aan zonlicht waren blootgesteld. Soms vertoondeV. dahliae interactie met droogte en bleken beide effecten minder dan optelbaar. De hoge waarden van de variatiecoëfficiënten maakten een groot aantal metingen per behandeling noodzakelijk; dit was vooral het geval bij metV. dahliae geïnfecteerde planten hetgeen aantoont datV. dahliae vooral in het begin van de groei niet alle bladeren in gelijke mate aantast. Door de matigende invloed van de integratie van alle bladlagen en mogelijk doordat de bovenste bladeren werden gestimuleerd, werd de totale gewasfotosynthese in mindere mate beïnvloed doorV. dahliae dan de individuele bladfotosynthese. De bovenste niet geïnfecteerde bladeren bleken verantwoordelijk voor het grootste gedeelte van de gewas-fotosynthese. De resultaten tonen aan, dat volgend op een infectie metV. dahliae, de fotosynthese reeds in een vroeg stadium van de groei wordt verminderd als een gevolg van droogtestress in de bladeren.
    Notes: Abstract Potato (Solanum tuberosum L.) plants cv.Saturna were subjected to infection withVerticillium dahliae and drought stress. At the early stages of growth, stomatal conductance, transpiration and net photosynthesis were measured at light saturation (PAR〉300 m−2) on individual leaves and with mobile field equipment with the aid of field enclosures. No significant changes in stomatal conductance and gas exchange characteristics occurred as a result ofV. dahliae instomatal conductance, transpiration and and photosynthetic rates, especially on older leaves and on plants exposed to direct sunlight for a longer period of time. In combination with drought,V. dahliae only occasionally showed interaction; their effects being less than additive. High values of coefficients of variatoon necessitated a high number of measurements per treatment; the more so in the inoculated plants which shows thatV. dahliae seems to affect certain leaves while not affecting others early in growth. Crop photosynthesis was less reduced byV. dahliae than individual leaf photosynthesis due to the levelling effect of integration over the whole canopy and possibly through a stimulation of the top leaves. The upper non-affected leaves are responsible for the bulk of photosynthetic crop activity. The results indicate that following an infection withV. dahliae photosynthesis is reduced early in growth as a result of drought stress in the leaves.
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  • 43
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    Wetlands ecology and management 4 (1996), S. 245-256 
    ISSN: 1572-9834
    Keywords: Anaerobiosis ; baldcypress ; cherrybark oak ; nuttall oak ; photosynthesis ; productivity ; soil redox potential ; stomatal conductance ; waterlogging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seedlings of baldcypress (Taxodium distichum), nuttall oak (Quercus nuttalli), and cherrybark oak (Quercus falcata var.pagodaefolia) were subjected to four flooding treatments: control, continuously flooded, intermittently flooded, and partially flooded for 70 days in a greenhouse. The treatments imposed various durations and intensities of soil redox potential (Eh) conditions representing a range encountered by plants in their habitats. Morphological changes and gas exchange responses to the treatments differed among the study species. Rapid development of adventitious root and hypertrophied lenticels were observed in baldcypress and nuttall oak under all flooded treatments. Cherrybark oak had the highest percentage reduction in net photosynthesis ranging from 65–87%, whereas reductions in nuttall oak ranged between 35–68% and in baldcypress between 6–21% in response to various treatments. Recovery of gas exchange was noted in baldcypress but no significant recovery was found in oaks. The recovery in baldcypress contributed to the continued growth and biomass accumulation under various treatments. Little evidence of consistent changes in biomass allocation patterns in response to the treatments was found in baldcypress but total biomass decreased significantly under the continuously flooded treatment. In oaks, total biomass decreased significantly in all flooded treatments. The present findings demonstrated that physiological functions are adversely affected by low soil Eh conditions and the extent of such effects are dependent on the intensity and duration of soil reduction as well as the species' capability to respond to such conditions rapidly. Management plans concerned with regeneration of bottomland forested ecosystems should consider the species flood response capabilities at seedling stages as well as the timing, durations, and intensities of soil reduction at the specific site.
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  • 44
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; genotypes ; interaction ; manganese ; oxidation ; take-all ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Take-all is a world-wide root-rotting disease of cereals. The causal organism of take-all of wheat is the soil-borne fungus Gaeumannomyces graminis var tritici (Ggt). No resistance to take-all, worthy of inclusion in a plant breeding programme, has been discovered in wheat but the severity of take-all is increased in host plants whose tissues are deficient for manganese (Mn). Take-all of wheat will be decreased by all techniques which lift Mn concentrations in shoots and roots of Mn-deficient hosts to adequate levels. Wheat seedlings were grown in a Mn-deficient calcareous sand in small pots and inoculated with four field isolates of Ggt. Infection by three virulent isolates was increased under conditions which were Mn deficient for the wheat host but infection by a weakly virulent isolate, already low, was further decreased. Only the three virulent isolates caused visible oxidation of Mn in vitro. The sensitivity of Ggt isolates to manganous ions in vitro did not explain the extent of infection they caused on wheat hosts. In a similar experiment four Australian wheat genotypes were grown in the same Mn-deficient calcareous sand and inoculated with one virulent isolate of Ggt. Two genotypes were inefficient at taking up manganese and were very susceptible to take-all, one was very efficient at taking up manganese and was resistant to take-all, and the fourth genotype was intermediate for both characters. All genotypes were equally resistant under Mn-adequate conditions.
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  • 45
    ISSN: 1573-5036
    Keywords: ammonium ; heterogeneity ; inflow ; localised nutrient ; mineralisation ; 15N ; nitrate ; organic residue ; proliferation ; roots ; soil ; uptake ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To obtain nutrients mineralised from organic matter in the soil, plants have to respond to its heterogeneous distribution. We measured the timing of nitrogen uptake by wheat from a localised, 15N labelled organic residue in soil, as well as the timing of changes in root length density. We calculated the rates of N uptake per unit root length (inflows) for roots growing through the residue and for the whole root system. A stimulated local inflow appeared to be the main mechanism of exploitation of the residue N during the first five days of exploitation. 8% of the N that the plants would ultimately obtain from the residue was captured in this period. Roots then proliferated in the residue. This, together with a rapidly declining N inflow, contributed to the capture, over the next seven days, of 63% of the N that the plants derived from the residue. After that time, massive root proliferation occurred in the residue, but relatively little further N was captured.
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  • 46
    ISSN: 1573-5036
    Keywords: evapotranspiration ; grain yield ; irrigation ; plant density ; water use efficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Yield of eight wheat cultivars was evaluated under rainfed and irrigated conditions in a Mediterranean environment. Variation in grain yield resulted from variation in both aboveground biomass production and in harvest index. Under rainfed compared to irrigated conditions, grain yield, biomass and days to heading were decreased, whereas harvest index was increased. Grain yield of the different cultivars under rainfed conditions correlated with that under irrigated conditions in one of the two years. Among cultivars, harvest index under rainfed and irrigated conditions were correlated in both years. Water was used more efficiently for biomass production, and equally efficiently for grain production, under irrigated compared to rainfed conditions. Under rainfed conditions, crop water use efficiency was higher for cultivars developed for rainfed environments than for those developed for high-rainfall or irrigated environments. Cultivars with low-rainfall target environments had the lowest evapotranspiration under rainfed conditions. Under rainfed conditions, differences between the cultivar groups in crop water use efficiency corresponded with trends in water use efficiency of individual plants and with the ratio of photosynthesis to transpiration, measured on plants grown in a growth room. Early in the season, water was used more efficiently for biomass production at high sowing densities than at low sowing densities. Through faster biomass production and ground cover a smaller proportion of the evapotranspired water was lost in soil evaporation and a larger proportion was transpired. However, the net effect was a greater water use in the early phases of growth and consequently a lower water availability later in the season, leading to similar yields regardless of sowing density.
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  • 47
    ISSN: 1573-5036
    Keywords: crown rot ; Fusarium ; fungal disease ; genotypes ; wheat ; zinc ; zinc efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A crown rot disease in wheat caused by the fungusFusarium graminearum Schw. Group 1 is a widespread problem in chronically Zn-deficient Australian soils. A link between crown rot and Zn deficiency was established by Sparrow and Graham (1988). This paper reports a test of a further hypothesis, that wheat genotypes more efficient at extracting zinc from low-zinc soils are more resistant to infection by this pathogen. Three wheat cultivars (Excalibur, Songlen and Durati) of differential Zn efficiency were tested at three zinc levels (0.05, 0.5 and 2.0 mg Zn kg−1 of soil) and three levels ofF. graminearum S. Group 1 inoculum (0.1 g and 0.3 g kg−1 live chaff-inoculum and control having 0.1 g kg−1 dead chaff inoculum). Six weeks after sowing dry matter production of shoots and roots was decreased byFusarium inoculation at 0.05 mg and 0.5 mg kg−1 applied Zn.Fusarium inoculum at 0.1 g was as effective as 0.3 g kg−1 for infection and decreasing dry matter. The infection at the basal part of culm decreased significantly by increasing the rate of Zn application. Excalibur, a Zn-efficient cultivar (tolerant to Zn deficiency) produced significantly more shoot and root dry matter, and showed less disease infection compared with Zn-inefficient cultivars (Durati and Songlen) at low (0.05 mg Zn kg−1 soil) and medium (0.5 mg Zn kg−1 soil) Zn fertilization rates. Higher rate of Zn fertilization (2.0 mg Zn kg−1 soil) reduced the disease level in Durati to the level of Excalibur but the disease level of Songlen was still high, indicating its high Zn requirement and or sensitivity to crown rot. The data on Zn uptake show that Excalibur, being Zn-efficient, was able to scavenge enough Zn from Zn-deficient soil, we suggest that besides sustaining growth Excalibur was able to build and maintain resistance to the pathogen; inefficient cultivars needed extra Zn fertilization to achieve performance comparable to that of Excalibur. The present study indicates that growing Zn-efficient cultivars of wheat along with judicious use of Zn fertilizer in Zn-deficient areas where crown rot is a problem may sustain wheat production by reducing the severity of the disease as well as by increasing the plant vigour through improved Zn nutrition. ei]Section editor: R Rodriques-Kalana
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  • 48
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    Euphytica 45 (1990), S. 71-80 
    ISSN: 1573-5060
    Keywords: wheat ; Triticum aestivum ; wheat leaf rust ; Puccinia recondita f.sp. tritici ; partial resistance ; histology ; growth curve
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The average size of wheat leaf rust colonies, measured using epifluorescence microscopy was significantly larger in the highly susceptible genotype Morocco than in the susceptible genotype Kaspar and the partially resistant genotypes Westphal 12A, Akabozu and BH 1146. This was already so three days after inoculation. Colony growth in partially resistant genotypes was continuously retarded compared to colonies in the highly susceptible genotype Morocco. No evidence was found for an initial inhibition of the growth of colonies in partially resistant genotypes. In partially resistant genotypes formation of uredial beds and sporulating areas started at a smaller colony size than in susceptible genotypes. Wheat leaf rust colonies in primary leaves of all genotypes studied were much larger than colonies in flag leaves measured at the same number of days after inoculation. Growth and sporulation of not intertwined colonies was not influenced by either a high or a low number of neighbouring colonies.
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  • 49
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    Euphytica 45 (1990), S. 81-86 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; wheat leaf rust ; partial resistance ; histology ; abortion ; adult plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Arrest of the growth of wheat leaf rust infection structures was studied with fluorescence microscopy in seedling leaves and flag leaves of the susceptible spring wheat genotypes Morocco and Kaspar and the partially resistant genotypes Westphal 12A and Akabozu. The percentages non-penetrants and substomatal vesicle abortion were low in all genotypes. In the partially resistant genotypes the percentage abortion of infection structures was higher than in the susceptible genotype Morocco. Aborted infection structures had formed one or two haustorial mother cells. In adult plants differences in the percentage aborted infection structures between susceptible and partially resistant genotypes were more pronounced than in seedlings. The so-called late abortion was not observed.
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  • 50
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    Euphytica 45 (1990), S. 59-69 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Pyrenophora tritici-repentis ; resistance ; tan spot ; yellow spot ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary No complete resistance to Pyrenophora tritici-repentis has been located in more than 1400 bread wheats examined. Incomplete resistance, however, occurs at different levels in many spring and winter types and data are presented for the strongest sources of resistance detected. In particular, there is a high frequency of Brazilian spring wheats with appreciable levels of resistance to this pathogen. Recent international nurseries from CIMMYT, Mexico, also contain numerous potentially valuable sources of resistance and these wheats may be shorter and higher yielding in Australia than the Brazilian wheats. The resistances in many Brazilian cultivars may be largely common because the cultivars are often strongly interrelated. Some of the Brazilian wheats resistant to P. tritici-repentis are also resistant to one or more of the septoria diseases and/or possess tolerance to aluminium toxicity.
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  • 51
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    Euphytica 45 (1990), S. 87-92 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; near-isogenic lines ; NILs ; Puccinia recondita f.sp. tritici ; leaf rust ; Puccinia striiformis ; yellow rust ; backcross ; variation ; background resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using the cultivar Arina as the recurrent parent, six backcrosses were made with two donor lines carrying the leaf rust resistance genes Lr1 and Lr9, respectively. Selection for leaf rust resistance occurred at the seedling stage in the greenhouse; the first plants transferred to the field were BC6F4s. Frequency distribution of the 332 Lr1/7 × Arina and the 335 Lr9/7 × Arina lines showed continuous variation for yellow rust resistance and heading date in these leaf rust near-isogenic lines (NILs). Similar results were also obtained for plant height, for resistance to powdery mildew and glume blotch, as well as for baking quality characters in another set of more advanced NILs. The available information on the behaviour of one of the parents of cultivar Arina led to the conclusion that the expressed yellow rust resistance is quantitative and might possibly be durable.
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  • 52
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    Euphytica 45 (1990), S. 169-177 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; coefficient of parentage ; pedigree ; gene pool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Pedigrees of 142 Yugoslavian winter wheat cultivars were traced to 110 ancestral genotypes, of which 41 contributed significantly. In each of the four major Yugoslavian wheat breeding programs, the most important ancestor, as evaluated by mean coefficient of parentage, was ‘Akagomughi’, source of the genes Rht8 and Ppd1. The other 13–19 ancestors accounting for the majority of the remaining germplasm, varied considerably among institutions. The relative contributions of ancestors changed little between the periods 1967–76 and 1982–86, with the exception of ‘Neuzucht’ (source of a 1B/1R translocation), which became much more important in the latter period.
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  • 53
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    Euphytica 46 (1990), S. 149-155 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; selection ; preharvest sprouting ; germination ; kernel color ; dormancy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The utility of spike- and seed-based mass selection techniques for improving preharvest sprouting resistance in heterogeneous wheat (Triticum spp.) populations was evaluated. Sorting seed by size improved selection efficiency in some cases, putatively by physiological synchronization. Progeny testing, as well as changes in frequency of red-kernelled types, indicate effectiveness of both spike- and seed-based mass selection for reduced preharvest sprouting. Differential effectiveness of mass selection, in populations segregating for dormancy from different sources, is consistent with previous work on mechanisms of dormancy from these sources. These results are of value to improvement of preharvest sprouting resistance in large, heterogeneous wheat populations.
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  • 54
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    Euphytica 47 (1990), S. 165-169 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; isozyme loci ; esterase ; homoeology relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The EST-6 leaf esterase phenotypes from euploid, nullisomic-tetrasomic and rye chromosome addition and substitution lines of common wheat were determined using polyacrylamide gel electrophoresis. Evidence is presented to demonstrate that Est-6 is a new set of genes, that are expressed in the leaf. The Est-6 gene set were clearly distinguished from the Est-5 genes which are expressed in the grain. The three homoeoallelic loci, Est-A6, Est-B6 and Est-D6, were located on chromosomes 3A, 3B and 3D. An Est-R6 gene was located on chromosome 6R is involved in rye. Some considerations concerning homoeology between homoeologous group 3 of wheat and the rye chromosome 6R are made.
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  • 55
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    Euphytica 48 (1990), S. 1-8 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; protein accumulation ; plant protein ; protein estimation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Better understanding of the physiological and genetic basis of wheat grain protein will contribute to breeding efforts for this characteristic. This study provides information about plant protein distribution in high and low grain protein winter wheats (Triticum aestivum L.) at different growth stages and its relation to grain protein. Field experiments involved two winter wheats with high grain protein, ‘Redwin’ and ‘Lancota’, and two with low grain protein, ‘Centurk’ and ‘Brule’ in two years. Protein content in the head, the upper three leaves, the first and second leaf, and the peduncle were estimated with Near Infrared Reflectance Spectrophotometer (NIR) at five growth stages. High protein cultivars had higher leaf protein at ripe and higher protein content in the heads at most growth stages than low grain protein cultivars. High protein cultivars had lower protein content in the peduncle than low protein cultivars at ripe. Correlation coefficients between plant-part protein and grain protein ranged from 0.48 to 0.87 for the heads, from −0.45 to −0.79 for the peduncle, and from 0.55 to 0.84 for the leaves. A combination of head, peduncle, and first leaf protein at heading was significantly related to grain protein (R2=0.71). Indirect selection for head, peduncle, and first leaf (flag leaf) protein at heading should result in increased grain protein. Recurrent selection for increased grain protein, with parent selectionbefore anthesis and hybridization should be successful.
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  • 56
    ISSN: 1573-5060
    Keywords: Sorghum bicolor ; sorghum ; Triticum aestivum ; wheat ; Oryza sativa ; rice ; Fraction-1-Protein inheritance ; Isoelectric focusing ; intergeneric hybrids ; Large and small sub-units ; rice × sorghum ; rice × wheat hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The polypeptide composition of Fraction-1-Protein (F1P) from rice × sorghum, rice × wheat hybrids and their respective parents have been analyzed by a microelectrofocusing method. The large sub-unit (LSU) is composed of three polypeptides and the small sub-unit (SSU) of two polypeptides in rice and sorghum parents and rice × sorghum hybrids. Similarly, LSU is composed of three polypeptides in the rice and wheat parents and rice × wheat hybrids. Two polypeptides occur in the SSU of rice parent and rice × wheat hybrids where as only one polypeptide in the wheat parent. These polypeptides also differ in their isoelectric points. Based on the previous reports of F1P inheritance in hybrids in other crops, F1P analysis of rice × sorghum and rice × wheat hybrids does not seem to be an important marker to identify such intergeneric hybrids. Since this is first such report of F1P inheritance in hybrids between distantly related plants, its implication in different modes of inheritance are discussed.
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  • 57
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    Euphytica 49 (1990), S. 155-159 
    ISSN: 1573-5060
    Keywords: Secale cereale ; rye ; rye-wheat-additions ; Triticum aestivum ; wheat ; anthocyanins ; purple leaf base ; purple/red auricles ; gene location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary ‘Purple leaf base’ is expressed only if there is anthocyanin pigmentation in coleoptiles either in rye or in rye-wheat-additions. Genes controlling ‘purple leaf base’ were found to be located on chromosomes 5R (An5), 4B (Ra2) and 6B (Ra3) using the trisomic set of rye cv. Esto and autoplasmic rye-wheat-additions, respectively.
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  • 58
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; drought resistance ; grain yield ; relative water content ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Water is often the most limiting factor to winter wheat (Triticum aestivum L.) production in the southern Great Plains of the U.S.A., yet the lack of reliable screening criteria has precluded direct selection for drought resistance in breeding programs. Previous work showed that leaf relative water content (RWC) was highly heritable when measured under field-drought conditions, but its adoption as a screening tool for yield improvement requires further investigation of the genetic relationship between grain yield and RWC. Plants representing high and low yield potential under drought stress, and a random group of plants, were selected from an F2 population having the pedigree, TAM W-101/Sturdy. Two sets of entries, each comprised of the two parents and 24 F2-derived lines, were evaluated under a rainshelter in the F3 (1986) and F4 (1987) generations to determine differences in leaf RWC during reproductive development. One set of entries did not receive any water after the jointing stage, and the other set was grown under well-watered conditions. A positive relationship was observed between grain yield and RWC measured during anthesis and mid-grain fill, as the high-yield selections maintained a significantly higher RWC than the low-yield selections. Grain yield and RWC were also positively associated among random selections segregating for both traits. Subsequent adjustment of genotype means for differences in reproductive development at time of sampling underscored the need to consider differences in maturity when RWC is the selection criterion.
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  • 59
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    Euphytica 50 (1990), S. 11-18 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Fusarium culmorum ; head blight ; scab ; resistance ; gene action ; number of genes ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crosses were made among ten winter wheat genotypes representing different levels of resistance to Fusarium head blight to obtain F1 and F2 generations. Parents, F1 and F2 were inoculated with one strain of Fusarium culmorum. Data on incidence of head blight 21 days after first inoculation were analyzed. Broad-sense heritabilities averaged 0.39 and ranged from 0.05 to 0.89 in the individual F2 families. The joint-scaling test indicated that the inheritance of Fusarium head blight resistance was adequately described by the additive-dominance model, with additive gene action being the most important factor of resistance. With respect to the non-additive effects, dominance of resistance predominated over recessiveness. The number of segregating genes governing resistance in the studied populations was estimated to vary between one and six. It was demonstrated that resistance genes differed between parents and affected resistance differently.
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  • 60
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    Euphytica 50 (1990), S. 1-9 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Fusarium culmorum ; Fusarium head blight ; resistance ; scab ; diallel cross ; combining ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten homozygous winter wheat genotypes representing different levels of resistance to Fusarium head blight were crossed in all possible combinations excluding reciprocals. Parents, F1 and F2 were inoculated with one pathogenic strain of Fusarium culmorum. Data for head blight, observed 21 days after first inoculation (OBS-2), and for the area under the disease progress curve, based on observations 14, 21 and 28 days after first inoculation (AUDPC), were analyzed. The contrast between parents and F1 crosses indicated dommance effects of the resistance genes. Diallel analysis according to Griffing's Method 4, Model 1 showed significant general combining ability (GCA) effects for both F1 and F2; specific combining ability effects were not significant. With the exception of one genotype for which general performance for Fusarium resistance was not in agreement with its GCA, the resistance to F. culmorum was uniformly transmitted to all offspring, and the parents can be described in terms of GCA. It is suggested that in the progenies with one of the awned lines as parent, one resistance gene was linked with the gene coding for presence of awns, located on chromosome 4B. A single observation date, taken at the right time, was as effective in assessing resistance as the AUDPC.
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    Euphytica 51 (1990), S. 77-86 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; dough stickiness ; rye-derived wheat cultivars
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rye-derived wheat cultivars are being used in many breeding programmes throughout the world in order to achieve improvements in yield and disease resistance. However, the serious quality defect of intense dough stickiness associated with many of these wheat cultivars is limiting the usefulness of their flour in large mechanised bread bakeries. A dough preparation procedure was developed which enabled the dough surface properties of a range of rye-derived wheat cultivars to be assessed on doughs mixed quantitatively to their optimum mixing time. Intense dough stickiness was found in samples of 1AL/1RS and 1DL/1RS translocation lines tested and in all of the 1BL/1RS wheat cultivars examined except the West German cultivar, Disponent. Most of the 1BL/1RS wheat cultivars were derived from the Russian cultivars, Kavkaz, Aurora and Skorospelka 35 and included the CIMMYT-bred cultivars such as the Veery lines (Glennson, Ures, Genaro and Seri) and the Nebraskan cultivar, Siouxland. Based on the results of studying selected 1BL/1RS wheat cultivars in detail, this intense dough stickiness appeared to be independent of growing season, trial location, protein content, mixing tolerance, milling process and extraction rate. In addition pilot bakery trials confirmed that our laboratory testing procedures can be used to detect this intense dough stickiness.
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  • 62
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    Euphytica 88 (1996), S. 165-174 
    ISSN: 1573-5060
    Keywords: wheat ; tritordeum ; Hordeum chilense ; Fusarium culmorum ; Septoria nodorum ; scab ; deoxynivalenol ; ergosterol ; resistance ; Triticum spp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hordeum chilense is a wild barley extensively used in wide crosses in the Triticeae. It could be a valuable source of resistance to Fusarium culmorum and Septoria nodorum. Some H. chilense x Triticum spp. amphiploids, named tritordeums, were more resistant than the parental wheat line to these diseases, others were not. Average contents of ergosterol and deoxynivalenol (DON) suggested that resistance to colonization by Fusarium was the highest for Hordeum chilense, followed by tritordeum and wheat in decreasing order. In particular, the H. chilense genotypes H7 and H17 enhanced the wheat resistance to F. culmorum in its tritordeum offsprings. Resistance to S. nodorum in tritordeum was not associated with tall plant height. There is sufficient genetic variation for resistance to F. culmorum and S. nodorum among tritordeum to allow the breeding of lines combining short straw and resistance to both diseases.
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  • 63
    ISSN: 1573-5060
    Keywords: Aegilops markgrafii ; aneuploids ; chromosome specific library ; DOP-PCR ; microdissection ; molecular markers ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We established a chromosome specific DNA library of the Aegilops markgrafii chromosome B. Eight microdissected chromosomes B obtained from a monosomic T. aestivum-Aegilops markgrafii addition line were PCR-amplified and the DNA was cloned in Escherichia coli DH5α. Clones were characterized by dot blot hybridization with total Ae. markgrafii DNA. 62% of clones represented repetitive sequences and 38% low or single copy sequences. The estimated length of excised inserts varied between less than 200 bp and more than 500 bp. The average size of inserts was 310 bp.
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  • 64
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    Euphytica 89 (1996), S. 1-10 
    ISSN: 1573-5060
    Keywords: aneuploidy ; chromosome substitutions ; height ; wheat ; yellow rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The difficulties in developing inter-varietal chromosome substitution lines in wheat are reviewed. The use of genetical, cytological and molecular markers is suggested as a way of overcoming them. These difficulties and the use of markers, as well as the need to develop duplicate lines to detect background variation, are described using the development and analysis of the Cappelle-Desprez (Bezostaya 1) chromosome substitution set as an example. The effects of substituting Bezostaya 1 chromosomes on final plant height and adult-plant resistance to yellow rust are reported. The large number of aneuploids and substitution lines available in wheat provides a tremendous, international resource, which should be exploited in the future.
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  • 65
    ISSN: 1573-5060
    Keywords: Bread-making quality ; chromosome effects ; grain hardness ; SDS-sedimentation volume ; substitution lines ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The group 1 and 6 inter-varietal chromosome substitution lines of Cappelle-Desprez (Bezostaya 1) were intercrossed along with the donor and recipient varieties, Cappelle-Desprez and Bezostaya 1, to give 36 genetically different families. The analysis of the means of these families showed that variation in SDS-sedimentation volume fitted a predominantly additive model. There were no significant within or between chromosome interactions among the group 1 and 6 chromosomes. Nor was there any evidence for interactions between these chromosomes and those of the background. Significant dominance/within chromosome interactions amongst the background chromosomes were however detected. Some of the positive effects on SDS-sedimentation were associated with increased grain hardness. Chromosome effects on % grain protein were not correlated with SDS-sedimentation.
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  • 66
    ISSN: 1573-5060
    Keywords: agronomic characters ; QTL ; RFLP maps ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The advent of molecular marker systems has made it possible to develop comparative genetic maps of the genomes of related species in the Triticeae. These maps are being applied to locate and evaluate allelic and homoeoallelic variation for major genes and quantitative trait loci within wheat, and to establish the pleiotropic effects of genes. Additionally, the known locations of genes in related species can direct searches for homoeologous variation in wheat and thus facilitate the identification of new genes. Examples of such analyses include the validation of the effects of Vrn1 on chromosome 5A on flowering time in different crosses within wheat; the indication of pleiotropic effects for stress responses by the Fr1 locus on chromosome 5A; the detection of homoeologous variation for protein content on the homoeologous Group 5 chromosomes; and the detection of a new photoperiod response gene Ppd-H1 in barley from homoeology with Ppd2 of wheat.
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  • 67
    ISSN: 1573-5060
    Keywords: aneuploids ; automated DNA sequencer ; chromosomal location ; microsatellite marker ; PCR ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The chromosomal assignment of 64 PCR-amplified microsatellite loci and 29 additional fragments amplified by the same primer pairs is described for bread wheat (Triticum aestivum). The distribution over the different chromosomes and chromosome arms appears to be random. The highest proportion of microsatellite loci is found on the B genome, followed by the A and D genome. About half of the primer pairs amplified unique fragments, while the other half amplified additional fragments. 25% of the primer pairs, mostly designed to clones of a PstI-library, amplify fragments on homoeologous chromosomes. In some cases, more than one fragment on a single chromosome or fragments on non-homoeologous chromosomes occurred. The use of an automated DNA sequencer accounts for the accurate resolution of multiple fragments and enables to differentiate between fragments, amplified by a single primer pair, with size differences as small as two base pairs.
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  • 68
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    Euphytica 89 (1996), S. 49-57 
    ISSN: 1573-5060
    Keywords: adaptability ; earliness per se ; photoperiod sensitivity ; vernalization sensitivity ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In order to obtain high levels of environmental adaptability in wheat varieties it is essential they flower at times appropriate to particular environmental conditions. The influence of three distinct genetic systems that together determine time of flowering is reviewed here. Vernalization genes are seen to be particularly important to winter wheats for their direct or indirect effects on winter hardiness. Vernalization genes play a minor role in determining flowering time in autumn sown winter wheats but insensitivity is essential if spring sown wheats are to flower. Day length sensitive photoperiod genes play a major role in determining flowering time and adaptability of autumn sown wheats. Insensitivity can promote yield advantages of over 35% in Southern European environments. 15% in Central Europe and offers benefits even in the UK. At present only a single allele of Ppd1 appears to have been introduced into commercial European wheat varieties. The merits of alternative Ppd1 alleles or different loci are discussed. The influence of earliness per se genes that determine flowering time independently of environmental stimuli is less well documented than the effect of photoperiod and vernalization genes. It is likely that genes on chromosomes belonging to groups 2, 3, 4, 6 and 7 may act to modify flowering time independently of environmental stimuli probably by determining numbers of vegetative and floral primordia being initiated or the rate of initiation of the primordia. Earliness per se genes appear to be widespread in European wheats and play a significant role in determining the exact time plants flower.
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  • 69
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    Euphytica 89 (1996), S. 69-75 
    ISSN: 1573-5060
    Keywords: dwarfing genes ; gene mapping ; GA insensitivity ; rye ; Secale cereale ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The improvement of lodging resistance by introducing major dwarfing genes, classified either as GA insensitive or GA sensitive, is one of the main strategies chosen by cereal breeders. In the present paper the current knowledge about the genetics, chromosomal localisation and the homoeoallelic relationships of the dwarfing genes in wheat and rye is reviewed. The confusing system of the symbolisation of the GA insensitive dwarfing genes/alleles in wheat is discussed and a nomenclature based on rules for gene symbolisation in wheat is proposed.
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  • 70
    ISSN: 1573-5060
    Keywords: Chromosomal location ; grain size ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Grain size in wheat is the most stable yield component and has a favorable effect on flour yield. To identify the chromosomes associated with the large grains of line G603-86, (grain weight over 60 mg and grain length of about 9 mm), F3 lines, extracted from F2 populations obtained from F1 monosomics of crosses between G603-86 (P1) and the monosomic set of Favorit (P2) were tested in the field. ANOVA showed significant differences among parents for grain weight and grain length, but not for grain width or the factor expressing the difference in grain form and density. Homoeologous groups had significant effects on grain weight and on all components of grain weight, while genomes were not significantly different for any of these characters. Grain weight was significantly increased by chromosomes 6D and 4A of G603-86. Grain length was significantly increased by chromosomes 4A, 4B, 2B, 3A and 1B, grain width by chromosomes 1A and 1B, and the factor form-density by chromosomes 6D and 6A. The high grain size in G603-86 results from the effects of genes located on many chromosomes which affect grain dimensions, form and density.
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  • 71
    ISSN: 1573-5060
    Keywords: chromosome substitutions ; hybrid dwarfness ; photoperiodic response ; tissue culture response ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The wheat varieties Chinese Spring, Marquis and Thatcher and five intervarietal ‘Chinese Spring’ substitution lines for chromosomes 2B (2 lines), 2D (2 lines) and 4A*, differing from the recipient variety in alleles for hybrid dwarfing genes and/or the photoperiodic response genes were analysed for tissue culture response (TCR). It could be demonstrated that only chromosome 2B has a major effect on TCR. Allelic variation at the hybrid dwarfing loci seems to have no effect on tissue culture performance, even in the combination D1D2D3 which gives the grass dwarf phenotype. Also the allelic constitution at the Ppd loci, gave no indication for a direct major effect of those alleles, however there seems to exist genetical factors for TCR on the homoeologous group 2 chromosomes which may be closely linked to the Ppd loci.
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  • 72
    ISSN: 1573-5060
    Keywords: Triticum miguschovae ; wheat ; leaf rust resistance ; transfer lines ; monosomic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Triticum miguschovae, a genome addition synthetic, was used as a source for transfer of leaf rust (Puccinia recondita tritici) resistance to common wheat. This synthetic, developed from two wild species Triticum militinae and Aegilops squarrosa, proves a valuable donor of the genes for leaf rust resistance. Leaf rust resistance was transferred from T. miguschovae by both dominant and recessive genes. Stable lines phenotypically similar to their recurrent parents Kavkaz and Bezostaya 1 but differing from them in a high level of leaf rust resistance were obtained. The genes for resistance in 3 selected lines differed from each other and from the known effective genes Lr9, Lr19, and Lr24. The resistance of one of them (line 1229) is controlled by two complementary interacting genes located on chromosome 7B and 1D was revealed by monosomic analysis.
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  • 73
    ISSN: 1573-5060
    Keywords: Aneuploidy ; dwarfing genes ; Triticum aestivum ; preferential transmission ; Aegilops sharonensis ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat varieties tend to be chromosomally unstable producing on average 2–3% of plants with abnormal chromosome numbers. A number of semi dwarf wheat varieties, carrying the gibberellic acid insensitive dwarfing genes Rht1 or Rht2, have been seen to produce distinct tall off types due to reduction in dosage of the chromosome carrying the dwarfing gene. The UK variety ‘Brigand’, carrying Rht2 on chromosome 4D, produced very distinct tall off types when this chromosome was reduced in dosage. The frequency of tall off types was sufficiently high to cause the variety to fail United Kingdom statutory uniformity tests. An attempt to prevent the loss of chromosome 4D was made by constructing translocation chromosomes involving the short arm of chromosome 4D, which carries Rht2, and the long arm of chromosome 4S l from Aegilops sharonensis, which carries a gene(s) conferring preferential transmission. The work in this paper describes the field evaluation of two lines carrying 4DS.4DL-4S l L translocations, and demonstrates their success in preventing spontaneously occurring monosomy of chromosome 4D in semi-dwarf wheats.
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  • 74
    ISSN: 1573-5060
    Keywords: C-banding ; FISH ; heterochromatin ; in situ hybridization ; rye ; Secale cereale ; Secale montanum ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The sequential combination of C-banding and in situ hybridization techniques applied in this or in a reverse order, are used to recognize targeted chromosomal regions in cereals. Both methods are described whereby standard chromosome squash preparations are followed by: i) C-banding technique using Leishman stain and a slightly modified in situ hybridization technique using biotin-labeled DNA probes, or ii) fluorescence in situ hybridization technique and C-banding. Both approaches have been successfully used onto mitotic chromosomes of rye and wheat resulting suitable for both their identification and detection of targeted sites.
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  • 75
    ISSN: 1573-5060
    Keywords: alien introduction ; chromosome pairing ; fluorescent in situ hybridization ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fluorescent in situ hybridization (FISH) of DNA to plant chromosomes has proved to be a powerful cytogenetic tool. The value of fluorescent in situ hybridization of total genomic DNA (GISH) of related species is demonstrated in the determination of wheat/alien chromosome pairing in hybrids. Its use for assessing the relative merits of the various genes that affect chromosome pairing is also shown. The ability of GISH to identify the presence in wheat of whole alien chromosomes or alien chromosome segments is illustrated. The potential of FISH for detecting repeated DNA sequences, low copy sequences and single copy genes is discussed.
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  • 76
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    Euphytica 89 (1996), S. 215-221 
    ISSN: 1573-5060
    Keywords: agronomic characteristics ; breeding trends ; quality ; year of release ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat breeding efforts for a half century in Japan were investigated by using of 129 varieties registered in MAFF from 1929 to 1984 in the view point of case history for three years. Varieties released in each breeding station are classified apparently by growth habit associated closely with duration of the cold requirement. Heading date is earlier in varieties released in southwest breeding stations (southern varieties) than in varieties released in northern breeding stations (northern varieties). Culm length is higher and the pre-harvest sprouting is more sensitive in northern varieties. In quality characters, northern varieties has the higher milling rate, lower apparent amylose content and larger particle size of flour. In varieties released in the Tohoku district, trend of breeding direction with time is smaller ear numbers and larger 1,000-grain weight and greater resistance to powdery mildew. In varieties released in the Kyushu district, a significant correlation with released year was clearly observed with regard to early maturity, short culm length, less grain crude protein content, less apparent amylose content and higher milling rate on breeding advancement.
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  • 77
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia striiformis ; stripe rust ; epidemiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten spring bread wheat cultivars with a susceptible seedling reaction to race 14E14 of yellow rust were tested at three locations to assess the level and stability of quantitative resistance. Quantitative resistance was expressed in terms of disease severity (DS), area under the disease progress curve (AUDPC), apparent infection rate (r), infection type (IT) and infection density. Large genotypic differences were observed for all variables measured. Morocco was the most susceptible cultivar. Based on its high infection type (IT=9) throughout the epidemics, it most likely does not possess any resistance. All other cultivars carry quantitative resistance. The levels ranged from very low (Taichung 23) to very high (Parula). Resistance levels were lower in Quito, Ecuador than at the other locations. Most likely, the lower temperatures in Quito resulted in a reduced expression of quantitative resistance to yellow rust and to obtain the same protection as at the other two locations, more resistance genes are needed. Therefore, to accumulate genes for quantitative resistance, Quito is considered to be the better location. Though significant cultivar-location interactions were detected, they were small compared to the cultivar and location effect. Therefore, they are considered of little importance and it is concluded that quantitative resistance is a stable trait, in the sense that cultivar rankings are hardly affected by environment. The contribution of infection growth to the development of yellow rust was demonstrated. Between 29 and 66% of the increase in disease severity could be contributed to growth of infections. These figures are probably an underestimation of the real contribution as new infections are very small, thus reducing the average size of infections and their contribution to the increase of disease severity.
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  • 78
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    Euphytica 91 (1996), S. 31-35 
    ISSN: 1573-5060
    Keywords: aluminum ; linkage map ; marker ; RFLP mapping ; Triticum aestivum ; Triticum turgidum ; wheat ; durum wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The tolerance of aluminum (Al) of disomic substitution lines having the chromosomes of the D genome of Triticum aestivum L. cv. Chinese Spring individually substituted for their homoeologues in T. turgidum L. cv. Langdon was investigated by the hematoxylin method. The disomic substitution lines involving chromosome 4D were more Al tolerant than Langdon. The tolerance was found to be controlled by a single dominant gene, designated Alt2, that is in the proximal region of the long arm of chromosome 4D. The locus was mapped relative to molecular markers utilizing a population of recombinant chromosomes from homoeologous recombination between Chinese Spring chromosome 4D and T. turgidum chromosome 4B. Comparison of the location of Alt2 in this map with a consensus map of chromosomes 4B and 4D based on homologous recombination indicated that Alt2 is in a vicinity of a 4 cM interval delineated by markers Xpsr914 and Xpsr1051. The Alt2 locus is distal to marker Xpsr39 and proximal to XksuC2. The Altw locus is also proximal to the Knal locus on chromosome 4D that controls K+/Na+ selectivity and salt tolerance. In two lines, Alt 2 and Knal were transferred on a single 4D segment into the long arm of T. turgidum chromosome 4B.
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  • 79
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    Euphytica 91 (1996), S. 127-140 
    ISSN: 1573-5060
    Keywords: In vitro selection ; salt tolerance ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Embryogenic calli isolated from immature embryos of four wheat cultivars were subjected to three in vitro selection methods for salt tolerance. The effect of NaCl on the selected and unselected cell lines has been investigated. The results indicated that the relative growth rate of callus decreased as the concentration of NaCl increased in both callus lines. The selected callus line gave a higher growth weight in the presence of NaCl in the medium and was highly significant as compared with unselected callus line across medium protocols in all wheat cultivars. The dry weight of both kinds of callus lines of all wheat cultivars increased markedly with increasing NaCl concentration in most cases. The Na+ and Cl- contents of both callus lines were increased with increasing salinity levels while K+ content was decreased. The selected callus line of each cultivar at the same salinity level produced significant amounts of Na+, K+ and Cl- higher than the unselected callus line in most salinity levels. However, the unselected callus lines of the cultivars Giza-157 and Sakha-90 at the same salinity level produced significant amounts of K+ higher than the selected callus line in most salinity levels. The proline content of both kinds of callus lines for all wheat cultivars was increased with increasing salinity level. However, the selected callus line gave a significantly higher proline content than the unselected callus line in all wheat cultivars at the same Salinity level. Results from the in vitro selection for NaCl tolerance showed that the stepwise method of increasing NaCl in the medium was more effective for plant regeneration than other methods.
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  • 80
    ISSN: 1573-5060
    Keywords: adaptation ; intrinsic earliness ; Triticum aestivum ; vernalization ; wheat ; Syria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study sought to identify factors that influence wheat development in the transitional wheat growing zone of northern Syria. Three development factors were studied, intrinsic earliness, and responses to vernalization and to photoperiod. Two sets of wheat were studied, each composed of lines with differing combinations of development factors. Set 1 comprised 20 parental and breeding lines utilized by the CIMMYT/ICARDA facultative and winter wheat breeding program based at Tel Hadya. Set 2 comprised 19 parental and breeding lines utilized by an Australian winter wheat breeding program based at Temora. Field development was recorded in greatest detail at one site. Tel Hadya, using the state of differentiation of the apex of the main tiller of sampled plants. To extend findings, development was also recorded as the time from sowing to ear emergence for later sowings of wheat at Tel Hadya, and in sowings at four other regional sites. The significance of each development factor was tested in multiple regressions that predicted either stage of apical development at Tel Hadya, or time to ear emergence in all trials. It was found that intrinsic earliness was the major factor associated with development, in both sets of wheat. Response to photoperiod had a much smaller and less consistent effect. Response to vernalization had least effect on development, possibly because low temperature in winter delayed development for a longer period than was required to fully vernalize winter wheats. Our results suggested it may not be directly relevant whether spring or winter wheats are grown in the transitional zone of northern Syria. The desired phenotype for the region, of slow development prior to double ridge, then fast development to ear emergence, cannot be simply achieved from combinations of the three development factors. Selection for improved adaptation to the region must continue to rely on direct field observations.
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  • 81
    ISSN: 1573-5079
    Keywords: cytochrome c ; photosynthesis ; photosynthetic bacteria ; electron transport ; Chloroflexus aurantiacus ; green bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The membrane-bound photooxidizable cytochrome c-554 from Chloroflexus aurantiacus has been purified. The purified protein runs as a single heme staining band on SDS-PAGE with an apparent molecular mass of 43 000 daltons. An extinction coefficient of 28 ± 1 mM−1 cm−1 per heme at 554 nm was found for the dithionite-reduced protein. The potentiometric titration of the hemes takes place over an extended range, showing clearly that the protein does not contain a single heme in a well-defined site. The titration can be fit to a Nernst curve with midpoint potentials at 0, +120, +220 and +300 mV vs the standard hydrogen electrode. Pyridine hemochrome analysis combined with a Lowry protein assay and the SDS-PAGE molecular weight indicates that there are a minimum of three, and probably four hemes per peptide. Amino acid analysis shows 5 histidine residues and 29% hydrophobic residues in the protein. This cytochrome appears to be functionally similar to the bound cytochrome from Rhodopseudomonas viridis. Both cytochrome c-554 from C. aurantiacus and the four-heme cytochrome c-558-553 from R. viridis appear to act as direct electron donors to the special bacteriochlorophyll pair of the photosynthetic reaction center. They have a similar content of hydrophobic amino acids, but differ in isoelectric point, thermodynamic characteristics, spectral properties, and in their ability to be photooxidized at low temperature.
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    Photosynthesis research 23 (1990), S. 67-72 
    ISSN: 1573-5079
    Keywords: leaf size ; light ; photosynthesis ; shading ; soybeans ; specific leaf weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study investigated the basis of the negative relationship between leaf size and photosynthetic rate per unit of area among five cultivars of soybeans. Exposure of developing mainstem leaves to light, and sizes and light saturated photosynthesis rates of those leaves at maturity were compared in cultivars grown in field plots for two years at Beltsville, Maryland, USA. Plants were grown both in stands at 2.5 cm by 1 m spacing and as isolated plants. While cultivar differences in leaf size were large and consistent in both planting arrangements, significant cultivar differences in light saturated photosynthetic rates were found only in plants grown in stands. Similarly, leaf size was significantly correlated with specific leaf weight only for plants grown in stands. The mainstem apex and developing mainstem leaves experienced more severe shading in large-leaved cultivars than in small-leaved cultivars when plants were grown in stands. Thus, cultivar differences in photosynthetic capacity were probably a consequence of differences in the exposure of developing leaves to light.
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  • 83
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    Photosynthesis research 23 (1990), S. 313-318 
    ISSN: 1573-5079
    Keywords: thylakoid membranes ; electron transfer ; photoacoustic spectroscopy ; energy storage ; photosynthesis ; plastoquinone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The photosynthetic energy storage yield of uncoupled thylakoid membranes was monitored by photoacoustic spectroscopy at various measuring beam intensities. The energy storage rate as evaluated by the half-saturation measuring beam intensity (i50) was inhibited by 3-(3,4-dichlorophenyl)-1,1 dimethylurea, by heat inactivation or by artificial electron acceptors specific for photosystem I or photosystem II; and was activated by electron donors to photosystem I. The reactions involving both photosystems were all characterized by a similar maximal energy storage yield of 16±2 percent. The data could be interpreted if we assumed that the energy storage elicited by the photosystems at 35 Hz is detected at the level of the plastoquinone pool.
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  • 84
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    Photosynthesis research 25 (1990), S. 77-82 
    ISSN: 1573-5079
    Keywords: autobiography ; chloroplast structure ; chloroplast proteins ; chloroplast lipids ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An autobiographic report of studies on the elucidation of the structure of the chloroplast is presented here. It deals with the discovery of the lamellar structure of chloroplasts by polarization-microscopy, the representation of their layer-like structure with the ultraviolet microscope and the results obtained by the electron-microscope leading to the discovery of the structural elements of the lamellar system. These lamellar structures were in the form of vesicles, and were named thylakoids. Isolation of the chloroplasts made it possible to determine their chemical composition. Amphiphilic lipids, together with water, create bimolecular layers and, therefore, are responsible for the structure of the thylakoid-membranes. The role of membrane proteins was emphasized. The isolation of the individual polypeptides was possible after dissociation in sodium-dodecyl-sulfate. Antisera to these polypeptides were used to localize them in the membrane. These antisera are able to inhibit special steps in the electron transport. Our results of the spectroscopic examination of photosynthetic membrane components are also discussed.
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    Euphytica 92 (1996), S. 147-153 
    ISSN: 1573-5060
    Keywords: breeding methodology ; drought patterns ; input-efficiency ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary About 32% of the 99 million ha wheat grown in developing countries experiences varying levels of drought stress. Three major drought types have been identified: Late drought (LD) is common in the Mediterranean region, early drought (ED) is found in Latin America and wheat is produced on residual soil moisture (RM) in the Indian subcontinent and part of Australia. Until 1983, CIMMYT selected all germplasm under near optimum conditions for its yield potential and tested only advanced lines under drought. In spite of many critics, this approach proved to be successful, since in the mid 80's CIMMYT germplasm was grown on 45% of the wheat area in LC with annual rainfall from 300–500 mm and on 21% in areas with less than 300 mm. Since 1983, CIMMYT's drought breeding methodology is to alternate segregating populations between drought stressed and fully irrigated conditions (FI) and to test advanced lines under a line source irrigation system. To compare the efficiency of these approach, yield of four, mostly leading varieties, from each of the regions with LD, ED, RM, and FI and twelve recent CIMMYT cultivars selected for high yield under FI and RM conditions (ALT) were compared under four different moisture regimes (FI, LD, ED, and RM) in 89–90 and 90–91 in Yaqui Valley, Mexico. Genotypic correlation between yield and days to flowering, days to maturity, height, grains m-2, TKW, test weight and grain fill period were calculated. Mean grain yield of the four best lines in the ALT group was highest under all moisture stress regimes, followed by the FI-group. However, the highest yielding cultivar within each moisture regime was from the FI-group under FI, from the LD-group under LD, and from the ALT-group under ED and RM conditions. Estimates for genetic advance suggest that FI is the best environment for increasing grain yield even in all three drought environments. This indicates that yield potential per se is beneficial also in drought environments. The highest yield in drought environments was realized by the CIM cultivars selected under FI and RM. Simultaneous evaluation of the germplasm under near optimum conditions, to utilize high heritabilities and identify lines with high yield potential, and under stress conditions to preserve alleles for drought tolerance seem at present the best strategy.
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  • 86
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    Euphytica 92 (1996), S. 221-224 
    ISSN: 1573-5060
    Keywords: hybrid lines ; nitrogen efficiency ; variation ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The new European Common Agricultural Policy and environmental considerations are certainly to change agricultural practices toward low input cultivation systems. Nitrogen is one of the main inputs of winter wheat in northern France and it contributes highly to phreatic water pollution. A research programme has then been set up in order to study whether it is possible to breed for winter wheat cultivars using more efficiently N fertilisers. Less nitrogen would be applied, decreasing pollution risks and operational costs. It has been shown that a large variation exists for N related traits and for the resistance against N deficiency. On the one hand the cv Arche is very resistant to N deficiency, its yield on low N conditions (with no N fertiliser) is on average 89% of its yield on high N conditions (with a high N application). On the other hand, cv Récital is very susceptible to N deficiency as this same percentage is only 61%. A study on 10 hybrids showed that heterosis for grain yield was higher at low N level than at high N level. This was due to a higher number of grains per m2.
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  • 87
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    Photosynthesis research 24 (1990), S. 109-113 
    ISSN: 1573-5079
    Keywords: atrazine ; diuron ; photosystem II ; photosynthesis ; thylakoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The binding of the herbicide atrazine to thylakoid membranes is often used to quantify Photosystem II reaction centres. Two atrazine binding sites, with high and low affinities, have been observed on the D1 and D2 polypeptides of Photosystem II, respectively (McCarthy S., Jursinic P. and Stemler A. (1988) Plant Physiol. 86S:46). We have observed that the accessibility of the low-affinity binding sites is variable, being limited in freshly isolated thylakoids or in fresh frozen-thawed thylakoids, but increasing during storage of the membranes on ice. In contrast, the accessibility of the high-affinity binding sites, which are titratable at low concentrations (〈 500 nM) of herbicide, is much less variable, although the dissociation constant is greatly influenced by ethanol. We conclude that to quantify Photosystem II reaction centres by atrazine binding, it is sufficient and more reliable to assay only the high-affinity binding sites.
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  • 88
    ISSN: 1573-5079
    Keywords: cyanobacteria ; photosynthesis ; psbC gene ; psbD gene ; translational start codon ; overlapping genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The unicellular photoheterotrophic cyanobacterium Synechococcus sp. PCC 7002 was shown to encode two genes for the Photosystem II reaction center core protein D2 and one gene for the reaction center chlorophyhll-binding protein CP43. These three genes were cloned and their DNA sequences determined along with their flanking DNA sequences. Northern hybridization experiments show that both genes which encode D2, psbD1 and psbD2, are expressed at roughly equivalent levels. For each of the two psbD genes, there are 18 nucleotide differences among the 1059 nucleotides which are translated. The DNA sequences surrounding the coding sequences are nearly 70% divergent. Despite the DNA sequence differences in the genes, the proteins encoded by the two genes are predicted to be identical. The proteins encoded by psbD1 and psbD2 are ∼92% homologous to other sequenced cyanobacterial psbD genes and ∼86% homologous to sequenced chloroplast-encoded psbD genes. The single gene for CP43, psbC, overlaps the 3′ end of psbD1 and is co-transcribed with it. Results from previous sequencing of psbC genes encoded by chloroplasts suggest that the 5′ end of the psbC gene overlaps the 3′ end of the coding sequence of psbD by ∼50 nucleotides. In Synechococcus sp. PCC 7002, the methionine codon previously proposed to be the start codon for psbC is replaced by an ACG (threonine) codon. We propose an alternative start for the psbC gene at a GTG codon 36 nucleotides downstream from the threonine codon. This GTG codon is preceded by a consensus E. coli-like ribosome binding sequence. Both the GTG start codon and its preceding ribosome binding sequence are conserved in all psbC genes sequenced from cyanobacteria and chloroplasts. This suggests that all psbC genes start at this alternative GTG codon. Based on this alternative start codon, the gene product is ∼85% identical to other cyanobacterial psbC gene products and ∼77% identical to eucaryotic chloroplast-encoded psbC gene products.
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  • 89
    ISSN: 1573-5079
    Keywords: inorganic carbon transport ; light ; Chlamydomonas ; CO2 exchange ; photorespiration ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of photon flux density on inorganic carbon accumulation and photosynthetic CO2 assimilation was determined by CO2 exchange studies at three, limiting CO2 concentrations with a ca-1 mutant of Chlamydomonas reinhardiii. This mutant accumulates a large internal inorganic carbon pool in the light which apparently is unavailable for photosynthetic assimilation. Although steady-state photosynthetic CO2 assimilation did not respond to the varying photon flux densities because of CO2 limitation, components of inorganic-carbon accumulation were not clearly light saturated even at 1100 μmol photons m-2 s-1, indicating a substantial energy requirement for inorganic carbon transport and accumulation. Steady-state photosynthetic CO2 assimilation responded to external CO2 concentrations but not to changing internal inorganic carbon concentrations, confirming that diffusion of CO2 into the cells supplies most of the CO2 for photosynthetic assimilation and that the internal inorganic carbon pool is essentially unavailable for photosynthetic assimilation. The estimated concentration of the internal inorganic carbon pool was found to be relatively insensitive to the external CO2 concentration over the small range tested, as would be expected if the concentration of this pool is limited by the internal to external inorganic carbon gradient. An attempt to use this CO2 exchange method to determine whether inorganic carbon accumulation and photosynthetic CO2 assimilation compete for energy at low photon flux densities proved inconclusive.
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  • 90
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    Photosynthesis research 25 (1990), S. 17-24 
    ISSN: 1573-5079
    Keywords: delayed light ; oxygen evolution ; photosynthesis ; thermoluminescence (money plant, pothos aurea leaf)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of ultraviolet light on thermoluminescence, oxygen evolution and the slow component of delayed light has been investigated in chloroplasts and Pothos leaves. All peaks including peak V (48°C) were inhibited by UV. However, the peak at 48°C which was induced by DCMU was enhanced following UV irradiation of chloroplasts at ambient temperature (23°C) whereas peak II (-12°C) and peak III (10°C) which were also induced by DCMU were inhibited. Chloroplasts treated with DCMU and dark incubated for several minutes at ambient temperature prior to recording of glow curves have also shown enhancement of peak at 48°C. A slow component of delayed light and photosystem II activity of chloroplasts were inhibited by UV whereas photosystem I activity was marginally affected. These results corroborate involvement of photosystem II in generating thermoluminescence and slow components of delayed light in photosynthetic materials.
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  • 91
    ISSN: 1573-5079
    Keywords: carbon dioxide ; Glycine max ; photosynthesis ; stomatal conductances ; water potential ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Soybean [Glycine max (L.) Merr. cv. Williams 82 and A3127] plants were grown in the field under long-term soil moisture deficit and irrigation to determine the effects of severe drought stress on the photosynthetic capacity of soybean leaves. Afternoon leaf water potentials, stomatal conductances, intercellular CO2 concentrations and CO2-assimilation rates for the two soil moisture treatments were compared during the pod elongation and seed enlargement stages of crop development. Leaf CO2-assimilation rates were measured with either ambient (340 μl CO2 l−1) or CO2-enriched (1800 μl CO2 l−1) air. Although seed yield and leaf area per plant were decreased an average of 48 and 31%, respectively, as a result of drought stress, leaf water potentials were reduced only an average of 0.27 MPa during the sampling period. Afternoon leaf CO2-assimilation rates measured with ambient air were decreased an average of 56 and 49% by soil moisture deficit for Williams 82 and A3127, respectively. The reductions in leaf photosynthesis of both cultivars were associated with similar decreases in leaf stomatal conductance and with small increases in leaf intercellular CO2 concentration. When the CO2-enriched air was used, similar afternoon leaf CO2-assimilation rates were found between the soil moisture treatments at each stage of crop development. These results suggest that photosynthetic capacity of soybean leaves is not reduced by severe soil moisture deficit when a stress develops gradually under field conditions.
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  • 92
    ISSN: 1573-5079
    Keywords: chloroplast coupling factor ; induction ; thioredoxin ; regulation ; ATP synthase ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Simultaneous, non-invasive measurements were made of the rate of photosynthetic CO2 fixation and the state of activation of the chloroplast CF1CF0-ATP synthase (CF) in field-grown sunflower (Helianthus annuus L.) during the dark-to-light transition at sunrise. CO2 fixation showed a linear response with light intensity from zero to about 500–700 μE m-2 s-1. However, at light intensities of only 5–22 μE m-2 s-1, the energetic threshold for activation of the CF was found to be significantly lowered (as compared to the pre-dawn state), presumably through reduction of the regulatory sulfhdryl groups of the γ-subunit of the CF. When these studies were extended to chamber-grown plants, it was found that as little as 5 seconds of illumination at 4 μE m-2 s-1 caused apparently full CF reduction. It is clear, therefore, that the catalytic activation of CF is not rate limiting to the induction of carbon assimilation under field conditions during a natural dark-to-light transition at sunrise. A model, based on the redox properties of the regulatory sulfhydryls, was developed to examine the significance of sulfhydryl midpoint potential in explaining the differences in light sensitivity and oxidation and reduction kinetics, between the CF and other thioredoxin-modulated chloroplast enzymes. Computer simulations of the light-induced regulation of three representative thioredoxin-modulated enzymes are presented.
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  • 93
    ISSN: 1573-5036
    Keywords: biological control ; Gaeumannomyces graminis var.tritici ; take-all ; Trichoderma harzianum ; T. koningii ; T. hamatum ; pyrones ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Antagonism tests on agar-plates and glasshouse screening indicated that three isolates ofTrichoderma harzianum varied in their ability to antagonize the take-all fungus (Gaeumannomyces graminis var.tritici). Isolate 71 which was the most effective in suppressing take-all of wheat, produced two pyrones and other undetermined analogues. Isolates ofT. koningii andT. hamatum shown to suppress take-all, produced a simple pyrone compound. AlthoughT. harzianum isolates 70 and 73 did not produce any pyrones, they reduced the disease albeit to a much lesser extent than isolate 71; with isolate 73 showing distinct host growth promotion effects. It is proposed that the success of isolate 71 ofT. harzianum was related to the pyrones it produces and that the ability of isolates 70 and 73 to reduce take-all may be related to mechanisms other than those involving antibiotics.
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  • 94
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    Plant and soil 123 (1990), S. 217-222 
    ISSN: 1573-5036
    Keywords: growth chamber ; nutrient solution ; 15NO3 assimilation ; photosynthesis ; relative growth rate ; seedlings ; short-term NaCl stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Short-term studies for comparing some primary metabolic and growth-responses to salt stress in seedlings of two maize genotypes differing in drought resistance were carried out under controlled conditions. Both genotypes revealed high yielding ability in favourable environments. Treatments: Control (Hoagland-Arnon No 1 solution) and salt stress (Hoagland-Arnon solution plus NaCl, Ψs = −0.84MPa). It was found that in both genotypes the activity of the principal metabolic pathway supplying reduced nitrogen (15N) for the synthesis of amino acids and proteins as well as the assimulatory number (14CO2—assimilation relation rate per chlorophyll unit) were decreased under the effect of the stress. These effects were more marked in the resistant genotype. In this genotype the stress induced metabolic activity decline was accompanied by a corresonding reduction of the relative growth rate. Conversely, continuing growth, resulting probably from accumulation of solutes, was observed in the susceptible genotype. On the basis of these and other observations it is assumed that the resistant genotype manifests short-term energy saving stress reactions.
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  • 95
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    Plant and soil 123 (1990), S. 223-227 
    ISSN: 1573-5036
    Keywords: aluminium ; accumulation ; aneuploid ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Preliminary studies indicated that aluminium-tolerance in wheat (Triticum aestivum L. Thell.) is a dominant character controlled by several genes. The present paper describes further work on localization and characterization of some of these genes in the genome of the medium Al tolerant wheat cultivar Chinese Spring (C.S.), using an aneuploid series (ditelosomics). Aluminium-tolerance of seedlings was assessed using the modified ‘pulse’ method; the aluminium concentration in the nutrient solution causing irreversible damage to the root apical meristems on exposure for 24 h at 25°C was the measure of Al-tolerance. At least three different factors controlling Al-tolerance in the C.S. cultivar were located on chromosomes 5As, 2Dl and 4Dl. Significant differences were found in Al-uptake and accumulation in roots of the respective ditelosomic lines and euploid seedlings of C.S. Genes controlling Al-tolerance located in the D genome (2Dl and 4Dl) were not expressed in solution culture when genes located on 5As were missing, whereas some tolerance was observed in aneuploid lines in which genes from 5As were present while genes from 2Dl and 4Dl were missing. It is concluded that Al-tolerance genes located in A genome control the expression of other Al-tolerance genes located in the D genome. The implications of the obtained results for chromosome and gene manipulations in cereals are discussed.
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  • 96
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    Plant and soil 124 (1990), S. 141-142 
    ISSN: 1573-5036
    Keywords: denitrification ; Gaeumannomyces graminis var. tritici ; potassium deficiency ; rhizosphere ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat inoculated with the root pathogen Gaeumannomyces graminis var. tritici (Ggt) was grown in quartz silt at two levels of potassium nutrition. While in plants well supplied with K the incidence of Ggt did not affect plant growth, it reduced shoot and root weight of K deficient plants. Denitrification, measured by the acetylene inhibition technique and expressed as N2O/mg root weight, was increased either by low K nutrition or by Ggt infection. Highest denitrification in the rhizosphere of plants was found with a combination of both, K deficiency and Ggt attack.
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  • 97
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    Plant and soil 128 (1990), S. 143-151 
    ISSN: 1573-5036
    Keywords: barley ; calcium ; humidity ; magnesium ; membrane leakage ; salinity ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Salinity-calcium interactions, which have been shown to be important in plants grown in dryland saline soils of the Canadian prairies, were studied in two species differing in salt tolerance. In solution culture, wheat showed a greater reduction in growth and a higher incidence of foliar Ca deficiency symptoms than barley when grown under MgSO4 or Na2SO4 plus MgSO4 salt stress. Amendment of the saline solution with Ca to increase the Ca/(Na+Mg) ratio ameliorated the effects of salt, but more so in wheat than in barley. At least part of the difference in salt tolerance between the two species must therefore relate to species differences in the interaction of salinity and Ca nutrition. The greater response of wheat to Ca was not due to a lower Ca status in leaf tissue; on the contrary, although Ca amendments improved tissue Ca/(Na+Mg) ratios in both species, salinized wheat had equivalent or higher Ca content, and higher Ca/(Na+Mg) ratios than did barley. The higher Ca requirement of wheat is apparently specific to a saline situation; at low salinity, wheat growth was not reduced as extensively as that of barley as Ca/(Na+Mg) ratio was decreased. High night-time humidity dramatically improved wheat growth under saline conditions, but increasing the Ca concentration of the saline solution had no effect on growth in the high humidity treatment. Membrane leakage from leaf tissue of wheat grown under saline conditions was increased compared to tissue from non-saline plants. Plants grown in Ca-amended saline solutions showed no increase in membrane leakage. These results confirm the importance of Ca interaction with salinity stress, and indicate differences in species response.
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  • 98
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    Plant and soil 178 (1996), S. 223-230 
    ISSN: 1573-5036
    Keywords: barley ; cultivar ; acquisition efficiency ; rhizosphere ; root hairs ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To assess the extent of variation in phosphorus acquisition efficiency of some winter wheat (Triticum aestivum L.), winter and spring barley (Hordeum vulgare L.) genotypes, depletion of inorganic phosphorus (P) extractable with 0.5 M NaHCO3 (NaHCO3-Pi) from the rhizosphere soil was studied. Nutrients supply, rhizosphere soil pH and soil water content was kept equal for all the genotypes with the aim to reduce the confounding variation due to these factors. The experimental set up implied that no difference in the relative growth rates, nitrogen, potassium and calcium content of shoot dry matter occurred among the genotypes. The winter wheat, winter barley and spring barley genotypes differed significantly (p〉0.05) in their efficiency to acquire NaHCO3-Pi from the rhizosphere soil. The efficiency of the winter wheat genotypes to acquire NaHCO3-Pi from rhizosphere soil ranked Kraka 〉 Gawain 〉 Foreman 〉 Sleipner = Obelisk 〉 Kosack 〉 Pepital 〉 Arum. Winter wheat genotypes differed in extent of P depletion profiles in the rhizosphere, indicating variation in root hair length. The winter barley and spring barley genotypes also showed significant differences in their P depletion profiles near roots. The efficiency of the winter barley genotypes to acquire soil P in the rhizosphere ranked Hamu 〉 Frost 〉 Marinka 〉 Astrid 〉 Clarine = Angora. The efficiency of spring barley genotypes to acquire NaHCO3-Pi in the rhizosphere ranked Canut 〉 Etna ≅ Riga 〉 Digger 〉 Peel 〉 Semal 〉 Alexis. The rhizosphere pH remained unchanged, suggesting that additional mechanisms such as root hair formation and root exudates play a significant role in causing variation in P acquisition among the genotypes.
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  • 99
    ISSN: 1573-5036
    Keywords: burr medic ; grass-infestation ; productivity ; re-establishment ; subterranean-clover ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field-plot experiment investigated the re-establishment and productivity in 1987 (following wheat (Triticum aestivum) in 1986) of burr medic (Medicago polymorpha) and subterranean (sub-) clover (Trifolium subterraneum), which were each sown with 0, 20, 40, 60, 80, or 100% grass in 1985. There was no difference in the amount of dry matter production by medic or sub-clover over the whole growing season but medic was more productive earlier and sub-clover more productive later. Grass generally had little effect on legume or total dry matter production at proportions 〈40%, though medic productivity was slightly more vulnerable to the effect of grass-infestation than sub-clover.
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  • 100
    ISSN: 1573-5036
    Keywords: barley ; chlorosis resistance ; cucumber ; genotypical differences ; grasses ; iron mobilization ; iron uptake ; maize ; microorganisms ; oat ; phytosiderophores ; rice ; root exudates ; root growth ; rye ; sorghum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Graminaceous species can enhance iron (Fe) acquisition from sparingly soluble inorganic Fe(III) compounds by release of phytosiderophores (PS) which mobilize Fe(III) by chelation. In most graminaceous species Fe deficiency increases the rate of PS release from roots by a factor of 10–20, but in some species, for example sorghum, this increase is much less. The chemical nature of PS can differ between species and even cultivars. The various PS are similarly effective as the microbial siderophore Desferal (ferrioxamine B methane sulfonate) in mobilizing Fe(III) from a calcareous soil. Under the same conditions the synthetic chelator DTPA (diaethylenetriamine pentaacetic acid) is ineffective. The rate of Fe(III)PS uptake by roots of graminaceous species increases by a factor of about 5 under Fe deficiency. In contrast, uptake of Fe from both synthetic and microbial Fe(III) chelates is much lower and not affected by the Fe nutritional status of the plants. This indicates that in graminaceous species under Fe deficiency a specific uptake system for FePS is activated. In contrast, the specific uptake system for FePS is absent in dicots. In a given graminaceous species the uptake rates of the various FePS are similar, but vary between species by a factor of upto 3. In sorghum, despite the low rate of PS release, the rate of FePS uptake is particularly high. The results indicate that release of PS and subsequent uptake of FePS are under different genetic control. The high susceptibility of sorghum to Fe deficiency (‘lime-chlorosis’) is most probably caused by low rates of PS release in the early seedling stage. Therefore in sorghum, and presumably other graminaceous species also, an increase in resistance to ‘lime chlorosis’ could be best achieved by breeding for cultivars with high rates of PS release. In corresponding screening procedures attention should be paid to the effects of iron nutritional status and daytime on PS release as well as on rapid microbial degradation of PS.
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