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  • Ultrastructure  (125)
  • paleolimnology  (54)
  • Springer  (179)
  • 1980-1984  (179)
  • 1925-1929
  • 1983  (128)
  • 1982  (51)
  • 1925
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  • Springer  (179)
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  • 1980-1984  (179)
  • 1925-1929
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  • 1
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    Springer
    Development genes and evolution 192 (1983), S. 42-44 
    ISSN: 1432-041X
    Keywords: Chick embryo ; Gastrulation ; Adenylate cyclase ; cAMP phosphodiesterase ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ultrastructural localization of adenylate cyclase (E.C. 4.6.1.1.) and cAMP phosphodiesterase (PDE) (E.C. 3.1.4.17.) in the ectoderm of the developmental stage 4 chick embryo was studied. Adenylate cyclase was localized in the lateral surfaces of the ectodermal cells. In the primitive streak cells the enzymatic activity was observed on all the lateral surfaces, whereas in the periphery of the blastoderm the reaction product was localized in the apical parts of the lateral plasma membranes only. cAMP PDE localized in the apical cytoplasm of the ectodermal cells, with highest activity in the globular projections.
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  • 2
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    Development genes and evolution 192 (1983), S. 171-178 
    ISSN: 1432-041X
    Keywords: Differentiation ; Digestive tract ; Endoderm ; Organ culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The self-differentiation potency of the endoderm of the chick embryo was investigated mainly by transmission electron microscopy. Endodermal fragments isolated from 4- to 6-day stomach or small intestine were cultured in the absence of mesenchyme and were able to differentiate in vitro into organ-specific epithelia. Endodermal fragments isolated from the stomach region differentiated into a pseudo-stratified epithelium with periodic acid Schiff-positive mucous granules in the apical cytoplasm, while those from the small intestinal region differentiated into a simple columnar epithelium with a striated border which was positive in alkaline phosphatase activity. These features are comparable with those of the mucous secretory epithelium of the normal embryonic stomach and the absorptive epithelium of normal embryonic small intestine, respectively. Next, the self-differentiation potencies were investigated of the upper and lower layers of the blastoderms, at stages 1–5 of Hamburger and Hamilton (H. and H.). Both stomach-type and small-intestine-type epithelia developed only when fragments of the lower layer isolated from the blastoderms older than stage 3 of H. and H. were cultured, suggesting that cells possessing the potency to differentiate into the stomach- and small-intestine-type epithelia exist in the definitive endoderm at the beginning of its formation.
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  • 3
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    Calcified tissue international 34 (1982), S. 273-279 
    ISSN: 1432-0827
    Keywords: Odontogenesis ; Ultrastructure ; Alkaline phosphatase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The ultrastructural localization and gradient of activity of alkaline phosphatase were studied with respect to cell differentiation, matrix synthesis, and matrix mineralization in the incisor and molar teeth of 4-day-old Sprague-Dawley rats. The animals were perfused intracardially at room temperature with 2.5% glutaraldehyde in 0.1M sodium cacodylate (pH 7.4) with 3–4% sucrose. The jaws were dissected, immersion-fixed for 24 h, and the incisor and molar tooth germs removed. These were demineralized in 10% EDTA in NaOH (pH 7.4) with 7% sucrose. After reactivation of the enzyme with 0.1M MgCl in Tris-maleate buffer (pH 7.4) at 4°C, the teeth were incubated for alkaline phosphatase in a medium consisting of 6 ml 3% sodiumβ-glycerophosphate, 4 ml 0.2M Tris-HCl buffer (pH 9.2), 3 ml 1.6% MgSO4, 12 ml 0.5% lead citrate (pH⋍12), and 2.1 g sucrose. The pH was adjusted to 9.2 with 0.2M HCl, the volume made up to 30 ml, and the solution centrifuged for 10 min at 5000 rpm. Control teeth were incubated in medium minus the substrate. Finally, the specimens were routinely post-fixed and embedded for sectioning and examination with a Philips 300 electron microscope. A gradient of alkaline phosphatase activity was mapped along the developing teeth in the cells of the stratum intermedium, the proximal borders of the ameloblasts, the early dentine matrix, the predentine-dentine border, matrix vesicles, and the plasma membranes of odontoblasts and subodontoblast cells. The gradient of alkaline phosphatase activity was evident in the forming tooth from the cervical loop to the crown apex and was related to the cellular events, matrix synthesis, and matrix mineralization occurring during odontogenesis.
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  • 4
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    Calcified tissue international 34 (1982), S. 382-390 
    ISSN: 1432-0827
    Keywords: Avian osteopetrosis ; Avian oncornavirus ; Ultrastructure ; Calcification ; Bone cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Diaphyseal tibial bone of 12.5 – 13-day and 19-day-old embryos and 20-day-old hatched chicks infected with retrovirus MAV.2-O were examined by transmission electron microscopy. The viruses were associated with lining osteoblasts and osteocytes. Whereas the infection of the osteoblast layer seemed to be a transient stage, virus association with osteocytes was a constant and main ultrastructural feature. The viruses were found either in the osteoid or in the periosteocytic space of the bone lacunae. They arose from dense cytoplasmic areas located near the cell plasmalemma via a budding process. The newly budded virus particles often had a large tail or a fine stalk-like process lost in the extracellular space. The viruses underwent calcification by deposition of inorganic material and were incorporated in the bone trabeculae. No production of virus was observed in typical osteoclasts with well-differentiated ruffled borders. The viral-induced avian osteopetrosis seemed to result from increased bone deposition through stimulation of osteoblast and osteocyte activities, whereas osteoclastic bone resorption seemed to be undisturbed.
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  • 5
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    Archives of microbiology 133 (1982), S. 11-19 
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Ultrastructure ; Mastigocladus laminosus ; Fischerella ; True branching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The morphology and ultrastructure of the thermophilic cyanobacteriumMastigocladus laminosus were examined by scanning and transmission electron microscopy. Mature cultures consisted of relatively old, wide filaments that branched frequently to form younger, thinner filaments. The cells of the younger filaments had a consistently cylindrical morphology, while those of older filaments were rounded and pleomorphic. The internal ultrastructure of the cells depended somewhat on their age. As young cells became larger and wider, their thylakoids underwent slight rearrangement and spread out toward the center of the cytoplasm. Polyphosphate bodies, carboxysomes (polyhedral bodies), and lipid-body-like structures increased in number as the cells aged, but ribosomes and cyanophycin granules were depleted. Cell division involved septum formation followed by ingrowth of the outer membrane and sheath. Cells in older filaments were separated from each other by a complete layer of sheath material. Septum formation in older cells was also seen to occur parallel to the long axis of the filament, thereby confirming that true branching took place.
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  • 6
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    Archives of microbiology 134 (1983), S. 295-298 
    ISSN: 1432-072X
    Keywords: Actinomycetes ; Streptomyces thermoviolaceus ; Sporogenesis ; Spore ornamentation ; Cupular knobs ; Sheath ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sporogenesis of aerial spores in Streptomyces thermoviolaceus corresponded to a common sporulation type in the genus. The sporulation septum was composed of an outer ring-shaped constriction wall and an inner interspace septum arising by the inwards growth of a double annulus. In mature spores the wall was composed of two layers, the outer one was part of the parent hyphal wall and septum material, the inner one was formed de novo. The spore chains were enclosed by the thin breakable sheath containing small rod-like elements. The ornamentation in the form of knobs, which were a characteristic feature of the species originated from the sheath. The knobs were hemispherical particles with an inner electron dense core and an outer electron transparent shell. The term “cupular knobs” was suggested for this type of tuberculate ornamentation. Frequently, the knobs became detached from the surface in which case the inner core separated easily from the shell.
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  • 7
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Ultrastructure ; Nitrogen fixation ; Water stress ; Taxonomy ; DNA ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two strains of desiccation-tolerant coccoid cyanobacteria, Chroococcus S24, a marine form, and Chroococcus N41, a cryptoendolith isolated from a hot-desert rock, have been characterized. The mol % DNA base compositions of the strains are 47.1 and 48.9% respectively. Plasmid DNA was not detected in either strain. The pigment contents and nutritional characteristics of the strains are identical. Both lack phycoerythrinoid pigments and, in culture, behave as slow-growing halotolerant marine forms with elevated requirements for Na+, Cl−, Mg2+ and Ca2+. Sucrose was the only carbon source of those tested that supported photoheterotrophic growth. Each strain synthesizes nitrogenase under anaerobic conditions but not in air. Morphologically the two strains are indistinguishable. They are considered to be independent isolates of the same cyanobacterial species. Chroococcus N41 was studied in detail with the electron microscope. When brought to equilibrium at matric water potentials of-168 MPa and lower (to-673 MPa=c0.12a w) the protoplast shrinks, but the cells maintain the same size and diameter as those at-2,156 kPa (MN medium; control); the sheath expands and remains attached to the cell wall outer membrane by fibrils. The cell wall, cell membrane, thylakoid membranes, cyanophycin granules and carboxysomes appeared intact in desiccated cells.
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  • 8
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    Cell & tissue research 234 (1983), S. 411-425 
    ISSN: 1432-0878
    Keywords: Intestines (chicken) ; Innervation ; Catecholamines ; Adrenergic fibres ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Axons in the duodenum, ileum and rectum of the domestic fowl were identified as catecholamine-containing (CA) on the basis of positive reactivity following chromaffin fixation for electron microscopy. CA-axons in association with blood vessels in all regions of the intestine and in non-vascular sites in the small intestine had a ‘ typical’ adrenergic appearance, in that they contained many small granular vesicles (SGV) and variable numbers of large granular vesicles (LGV). In the rectum the non-vascular CA-axon profiles were atypical, in that there were many elongated LGV and few SGV, and the chromaffin reactivity was weak. The nerve profiles in the rectum were dramatically reduced following 6-hydroxydopamine and reserpine treatment and were absent in rectum cultured in the absence of extrinsic ganglia. It was concluded that the profiles, in spite of their low chromaffin reactivity, truely represent CA-axons. The possibility was raised that the atypical morphology and reduced chromaffin reactivity is due to the presence of adrenaline.
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  • 9
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    Cell & tissue research 234 (1983), S. 427-437 
    ISSN: 1432-0878
    Keywords: Corticotropes ; Rat fetus ; Ultrastructure ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Corticotropes of rat fetuses aged 16, 18 and 21 days were localized by the indirect antibody-enzyme method on semithin sections of the pituitary. The development of the ultrastructure of these cells was observed on consecutive ultrathin sections. In comparison with previous data our present results show that identification of a fetal cell type cannot be based entirely on morphological criteria. The structural peculiarities of corticotropes obtained from studies in vivo are compared with those observed in cells maintained in vitro.
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  • 10
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    Cell & tissue research 234 (1983), S. 679-689 
    ISSN: 1432-0878
    Keywords: Odontogenesis ; Rats ; Cyclophosphamide ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cyclophosphamide-induced changes in rodent odontogenesis were investigated by light and electron microscopy in four-day-old Sprague Dawley rats given one injection of 40 mg/kg of body weight of cyclophosphamide and killed at intervals of one hour, one day, one week and two weeks. Incisor and molar teeth were dissected from the animals, fixed in 2.0% glutaraldehyde in 0.1 M sodium cacodylate with 3.4% sucrose, and subsequently some were incubated for alkaline phosphatase reaction, and embedded in Spurr's medium for sectioning at light- and electron-microscopic levels. From three days a cell-sparse zone was created in the pulp in the growing end of the tooth and progressive cellular changes were observed which became more severe in the one-week and two-week specimens. Subodontoblast and adjacent pulpal cells were the most affected showing nuclear changes, damage to, or loss of, organelles, and inclusion bodies. Odontogenic epithelium was less affected and odontoblasts appeared to be unaffected by the drug. A new irregular matrix was laid down in the defect area and seemed to be the product of depolarized odontoblasts. This new matrix showed alkaline phosphatase activity, as did the cells embedded in it, and later it became mineralized. It is speculated that the polarity of odontoblasts might be maintained by an intact subodontoblastic layer; when this is lost the odontoblasts become depolarized and capable of secreting matrix from both ends.
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  • 11
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    Cell & tissue research 232 (1983), S. 579-591 
    ISSN: 1432-0878
    Keywords: Cavernous bodies ; Endothelial cells ; Respiratory activity ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Work on endothelial cells has been limited by the availability of procedures for obtaining such cells in quantities adequate for direct in vitro analysis. The present paper describes a method for the isolation of endothelial cells from bovine cavernous bodies. A number of cells ranging from 2.5 to 4 × 108 per animal has been obtained. The cells were identified as follows 1) presence of the “Weibel and Palade” bodies in the isolated cells, 2) “cobblestone” appearance of cell cultures, and 3) presence of factor VIII, as demonstrated by immunofluorescence assays. The cell viability at the end of the purification procedure was tested 1) by dye-exclusion tests and 2) by metabolic assays. Features of this preparation are 1) the very high yield of viable endothelial cells, 2) the absence of contamination by fibroblasts and smooth muscle cells and a very low contamination by erythrocytes and 3) the fine dispersion of the isolated cells. These properties allow functional and subcellular fractionation studies on freshly isolated endothelial cells of microvascular origin.
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  • 12
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    Cell & tissue research 233 (1983), S. 427-438 
    ISSN: 1432-0878
    Keywords: Myocardium ; Thunnus alalunga ; Ultrastructure ; Mitochondria ; Myofibrils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The common ventricle in the heart of the Thunnus alalunga was studied. The ventricular myocardium consists of an outer compact layer and a thick inner spongy layer. The compact layer has slightly larger cells (4–6 μm diameter) than the spongy layer (2.5–5 μm diameter). Ultrastructurally the myocardium displays normal arrangements of myofibrils and mitochondria. The sarcoplasmic reticulum is poorly developed. The intercalated discs are simple with the fascia adherens being the most frequent junctional type observed; occasionally a desmosome was seen. Nexus type junctions are present but are unassociated with the intercalated discs. There are no t-tubules evident but the plasmalemma exhibits numerous caveolae which rarely form couplings with the sarcoplasmic reticulum. A morphometric analysis of the volume percent of mitochondria and myofibrils showed that the myocardial cells in the spongy layer of the heart have a significantly greater volume percentage of mitochondria than the compact layer. No significant differences were found between myocardial regions when the volume percentages of myofibrils were compared. The physiological studies revealed that the albacore tuna has heart rates (120 bpm) and ventricular blood pressures (100 mmHg) that are among the highest reported for fish.
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  • 13
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    Cell & tissue research 233 (1983), S. 523-537 
    ISSN: 1432-0878
    Keywords: Epididymis (rat) ; Myosin ; Actin ; Fibronectin ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The anatomical distribution of smooth muscle actin, myosin, fibronectin and basement membrane has been investigated immunohistochemically, using the indirect immunofluorescence technique, in the rat epididymis. The findings were correlated with the ultrastructural organization of the organ. Actin was found to be distributed in the stereociliary region of the epithelial principal cells and in the terminal web region. Actin was also visible along the base of the epithelium. Myosin was detected in the terminal web and in the terminal bar regions of the epithelium. The contractile cells showed a strong stain for both proteins. Basement membrane immunoreactivity was distributed along the epithelial basement membrane and around the contractile cells of the wall. In the cauda, between the epithelium and the contractile cell layers, the lamina propria, containing blood vessels and a thin layer of cells, was negative for all antigens investigated. Fibronectin showed a granular distribution around the contractile cells, mainly in the cauda. The ultrastructural study showed only thin (5–6 nm in diameter) filaments in the stereocilia and terminal web region. Thin filaments were also visible in the cytoplasm of the basal cells, thus suggesting a contractile function of this cell type. The heterogeneous appearance of the contractile cells of the wall seemed to support the different contractile pattern of the epididymal regions: caput, corpus and cauda. The cells present in the lamina propria showed cytoplasmic vesicles with dark granules resembling the “A” cell granules of the endocrine pancreas and gut mucosa cells.
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  • 14
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    Cell & tissue research 233 (1983), S. 563-577 
    ISSN: 1432-0878
    Keywords: Nematocysts ; Structure ; Ultrastructure ; Function ; Cubozoa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Microbasic p-mastigophores, euryteles of two size groups, holotrichous isorhizas and atrichous isorhizas, comprise the cnidom of Chironex fleckeri, a cubozoan that has been responsible for several human fatalities. In its undischarged state each microbasic mastigophore of C. fleckeri consists of a capsule containing matrix and an inverted tube possessing a smooth-walled butt which is loosely coiled helically and which narrows to form a thread that is tightly coiled helically and markedly pleated. Both butt and thread carry three helices of spines and contain a granular matrix. During discharge, the proximal butt spines form initially a piercing stylet. Granular material from the butt and thread is released prior to the release of capsular material. Each eurytele possesses a tube with a butt composed of three bulbs, the middle bulb bearing long spines. Each holotrichous isorhiza possesses a coiled tube bearing small spines along its length. Each atrichous isorhiza exhibits a tube that is devoid of spines and loosely folded in the undischarged condition. The probable role of each type of nematocyst is inferred from its structure and features that enable the ready separation of the nematocysts of C. fleckeri from those of scyphozoan jellyfish are discussed.
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  • 15
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    Cell & tissue research 233 (1983), S. 629-638 
    ISSN: 1432-0878
    Keywords: Ciliary process ; Cultured explant ; Epithelial cells ; Ultrastructure ; Bovine and rabbit eyes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural characteristics of ciliary epithelium from bovine, pigmented rabbit, and fetal albino rabbit were studied in cultured explants. The tips of ciliary processes were cultured in plastic dishes with Dulbecco Modified Eagle Medium (DMEM) containing 5% fetal bovine serum. More than half of the expiants adhered to the plastic culture dish, and epithelial cells spread as monolayers within a few days. Initially the explant contains two layers, the outer (nonpigmented cells) and the inner (pigmented cells). Later the explant exhibits three layers: 1) outermost lightly pigmented flattened cells, 2) an outer layer of nonpigmented cells, and 3) an inner layer of densely pigmented cuboidal cells. The cells of the outermost layer are continuous with the cells of the inner layer. A narrow space lies between the outermost layer and the outer layer. The columnar cells in the outer layer contain well developed organelles but no pigment granules; they possess a basement membrane, lateral interdigitations, and junctional complexes near their apices. Numerous focal junctions and some ciliary channel-like structures were detected between the columnar cells of the outer layer and the cuboidal cells of the inner layer. The cuboidal cells of the inner layer are filled with pigment granules. These observations suggest that the columnar cells of the outer layer are nonpigmented epithelium, the cuboidal cells of the inner layer are pigmented epithelium, and the flatened cells in the outermost layer are derived from pigmented epithelium.
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  • 16
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    Cell & tissue research 233 (1983), S. 693-698 
    ISSN: 1432-0878
    Keywords: Thyroid gland ; Human fetus ; Follicles ; Ultrastructure ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Thyroid glands from 40 human fetuses ranging from 30 to 175 mm in length were examined by electron microscopy. At the 30 mm stage, the cells are arranged in cords or clusters and contain numerous free ribosomes. The rough-surfaced endoplasmic reticulum (RER) is poorly developed and the Golgi complex consists of saccules, smooth and coated vesicles. Mitochondria, microfilaments, glycogen particles, occasional lysosome-like structures, and single cilia are observed. At sites of incipient follicle formation, intercellular clefts containing interdigitating microvilli, delimited by junctional complexes, are evident. In addition, the apical region of some cells contains vacuoles. At the 50-mm stage, intercellular clefts are more apparent, some of them organized into small follicles through the enlargement of the lumen, the incorporation of additional cells, and the formation of a basal lamina. With increasing gestational age, more follicles are established. The lumina vary from round to clover-leaf shaped and have an electron-dense content. Cisternae of RER are dilated and contain flocculent material. The Golgi complex is more extensive, and lysosome-like structures are more numerous. By the 100-mm stage, the follicular cells appear morphologically well differentiated. The results demonstrate an extracellular origin of the follicular lumen in the human fetal thyroid.
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  • 17
    ISSN: 1432-0878
    Keywords: Fibronectin ; Lung ; Development ; Ultrastructure ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In a previous study changes in the macrodistribution of fibronectin during rat-lung development were examined. Using the peroxidase-antiperoxidase immunocytochemical technique, we have demonstrated the presence of fibronectin in embryonic, neonatal, and adult rat lung at the ultrastructural level. In the embryo, fibronectin is found both in an intra-and extracellular association with isolated pneumoblasts, and in a periodic distribution along the basal lamina. The neonate displays fibronectin in an intracellular association with early type-I cells and on their basal and luminal surfaces, but not in association with type-II cells. Neonatal basal lamina is diffusely labeled by anti-fibronectin antiserum. Fibronectin in adult tissue is found both intracellularly and on the basal and luminal surfaces of type-I cells but not in type-II cells. The basal lamina and interstitial connective tissue are slightly or non-reactive. These observations confirm and extend our initial suggestion that fibronectin is involved in rat-lung development.
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  • 18
    ISSN: 1432-0878
    Keywords: Sea star ; Development ; Cuticle ; Extracellular materials ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of conspicuous extracellular materials during the life history of a sea star (Patiria miniata) is described. The outer surface of the developing sea star is covered by two morphologically different cuticles that appear sequentially during ontogeny. The primary cuticle, which is about 120 nm thick and two-layered, is present from mid-blastula through the end of the larval stage. The secondary cuticle, which is about 1 μm thick and three-layered, first appears on the epidermis of the rudiment region of the larva and, after metamorphosis, covers the entire epidermis of the juvenile and adult stages. During ontogeny, there are only two conspicuous gut cuticles: the first lines the newly invaginated archenteron at the start of the gastrula stage, and the second lines the esophagus during the larval stage. A blastocoelic basal lamina first appears at mid-blastula and persists as subectodermal and subendodermal basal laminae. Ruthenium red-positive granules are detectable between the lateral surfaces of adjacent ectodermal cells during part of the gastrula stage; this transient intercellular material may possibly aid in lateral adhesion between cells.
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  • 19
    ISSN: 1615-6102
    Keywords: Anemia phyllitidis ; Chloroplast ; Gametophyte ; Metronidazole ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Metronidazole inhibits cell division in gametophytes of the fernAnemia phyllitidis without changing morphogenetic patterns. Simultaneously the sensitivity of the prothallia against gibberellins which substitute for the natural sexual pheromone “antheridiogen A”, is increased. The inhibition of cell division is accompanied by a loss of chlorophyll and by severe changes in the ultra-structure of the chloroplasts.
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  • 20
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    Protoplasma 113 (1982), S. 189-192 
    ISSN: 1615-6102
    Keywords: Brassica napus ; Membrane ; Nucleolus ; Nucleus ; Ultrastructure ; Vacuole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Vacuole-like structures were found in the nuclei of root tip cells ofBrassica napus. The cells containing the unusual nuclear inclusions were found to be adjacent to zones of degenerating cells. Such groups of cells occurred irregularly in the meristematic regions of the young root tips. The possibility that they represent changes which have occurred in old seeds is discussed. The “vacuole-like” structures seen in the cells adjacent to the degenerating zones were bounded by a membranous layer 12 nm thick. This is thicker than most cellular membranes. The “vacuoles” frequently contained inclusions and showed similarities to protein bodies reported elsewhere. The structures are thought to represent rearrangements of cell products which may have accumulated through an imbalance of metabolism in consequence of the imminent cell degeneration.
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  • 21
    ISSN: 1615-6102
    Keywords: Aspergillus nidulans ; Aspergillus giganteus ; Hyphal apex ; Ultrastructure ; Woronin body
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The hyphal tip ultrastructure ofAspergillus nidulans andAspergillus giganteus indicates that their apical organization is very similar to that found in other filamentous fungi. Both species have an area immediately behind the hyphal apex free of all large organelles and containing a high concentration of vesicles. InA. giganteus only one size class of vesicle is clearly evident, with a mean diameter of 72 nm. InA. nidulans two size classes of vesicle were found, with mean diameters of 75 nm and 31 nm. A Spitzenkörper is evident inA. nidulans as an area very close to the tip containing only the smaller vesicles. InA. giganteus one or more apparently mature Woronin bodies were found within the first 1 μm of some hyphal apices. The possible significance of their presence is discussed.
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  • 22
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    Protoplasma 114 (1983), S. 85-92 
    ISSN: 1615-6102
    Keywords: Chloroplasts ; Iron stress ; Sugar beet ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When grown in iron-free media, the youngest leaves of healthy green sugar beet plants became completely yellow after 6 to 8 days. This chlorosis was quickly reversed by resupplying iron. A study of the ultrastructure of the iron -stressed leaves revealed apparently normal subcellular organization except for the plastids which were small and undeveloped, contained a rudimentary, disorganized grana-fretwork and clusters of vesicles in the periphery. Twelve to 16 hours after resupply of iron, aggregates of phytoferritin were observed in the stroma, and the granal fretwork underwent further development. There was an increased orientation of the membranes along the long axis of the plastids and an increase in the length of the individual grana stacks. By 48 hours, leaf chlorophyll content was about 40% of the control. At the ultrastructural level, parallel alignment of membrane orientation was complete and the grana stacks began to increase in the number of thylakoids per stack.
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  • 23
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    Protoplasma 115 (1983), S. 1-10 
    ISSN: 1615-6102
    Keywords: Brassica napus ; Nucleolus ; Pisum sativum ; Ultrastructure ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The karyosome is a spherical body up to 1 μm in diameter that lies on the nucleolus of certain plant species, particularly those with a relatively low nuclear DNA content and an areticulate nuclear structure. It can be seen in the light microscope after impregnation with silver; in the electron microscope its structure consists of fibrillo-granular material. Nucleoli of cells in root apices may bear 0, 1, or 2 karyosomes. The frequency with which these numbers of karyosomes are observed depends on the location of the cells within the apex. In roots ofPisum sativum andZea mays the nucleoli of both slowly-dividing and young differentiating cells bear karyosomes more frequently than the nucleoli of rapidly-dividing cells. The karyosome seems to adopt a preferred location on the nucleolus, lying most frequently on the nucleolar surfaces directed towards the apex or base of the root. The origin and functional significance of the karyosome are discussed. Morphological evidence suggests that it may be material that formerly was part of a fibrillar centre.
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    Protoplasma 116 (1983), S. 1-13 
    ISSN: 1615-6102
    Keywords: Cuticle ; Peristomatal transpiration ; Stomata ; Ultrastructure ; Funaria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cuticle and pore development in the guard cells ofFunaria were investigated with the electron microscope. Pore cuticle formation is simultaneous with the creation of the pore itself. The morphology of the pore cuticle is unlike that of any cuticle described in the literature. It has many lamellae which are penetrated by electron dense fibrils. Three different cuticular morphologies exist from the pore to the subsidiary cell walls. The cuticles on the pore and outer walls contain fibrils that sometimes reach to the surface. The subsidiary cell cuticle lacks fibrils altogether. It is hypothesized that (1) cuticularization of the middle lamella contributes to ventral wall separation and (2) differences in extent of cuticular fibrils are related to greater water loss from stomata than from subsidiary cells (peristomatal transpiration).
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  • 25
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    Protoplasma 116 (1983), S. 115-124 
    ISSN: 1615-6102
    Keywords: Microtubules ; Moss ; MTOC ; Sporogenesis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Microtubule systems appear sequentially at the distal and proximal poles of tetrad members during mid-sporogenesis in the mossTetraphis pellucida Hedw. The distal microtubule system emanates from a microtubule organizing center (MTOC) located between the single plastid and the nucleus. The distal MTOC and associated microtubules, which appear immediately after cytokinesis, are ephemeral and do not appear to be associated with the deposition of exine occuring at the same time. The proximal microtubule system, which appears slightly later than the distal system, is a more stable component of mid-sporogenesis. The proximal MTOC is an irregularly lobed, patelliform aggregation of electron-dense granules located beneath the plasma membrane at the proximal spore pole. Several bundles of microtubules radiate from the proximal MTOC and traverse the cell, enclosing the nucleus in an cone of microtubules. The proximal microtubule system is thought to function in aperture development and organelle migration. The relatively large nucleus migrates a short distance in the small spore early in the tetrad stage and maintains its acentric position at the proximal pole throughout later stages of sporogenesis. The plastid migrates later in the tetrad stage from its meiotic position parallel to the distal surface to a position perpendicular to the distal surface with one tip in close proximity to the proximal MTOC. The proximal microtubule system reaches its maximum development by the end of the tetrad stage and all micrographic evidence of it is lost in the maturation stages of late sporogenesis.
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  • 26
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    Protoplasma 116 (1983), S. 99-114 
    ISSN: 1615-6102
    Keywords: Sphaerostilbe repens ; Ultrastructure ; Differentiation ; Coremia ; Rhizomorphs
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    Topics: Biology
    Notes: Summary The differentiating stages of coremia and rhizomorphs inSphaerostilbe repens were studied by transmission electron microscopy. Vegetative mycelium is characterized by highly cytoplasmic cells rich in ribosomes and mitochondria and with few vacuoles as well as endoplasmic reticulum. Cell walls are thin attaining a maximum thickness of 0.10 μm. During the aggregating phase a prosenchymatous mass of randomly oriented cells is produced by localized elongation and branching of the filaments. The hyphae in this region have the appearance of actively metabolising cells. In the course of the differentiating phase, numerous hyphae of the median zone of the aggregate grow upward and downward to give rise to coremium and rhizomorph primordia respectively. The individual hyphal tips lay parallel to each other and cells of the growing apices retain their meristematic characteristics. At the periphery of the aggregate and to a lesser extent in the subapical rhizomorphic zone, cells reduce their cytoplasmic density as a consequence of a decrease in the number of ribosomes. These cells also increase in size and become isodiametric and vacuolated. During cellular differentiation walls increase steadily in thickness and at the elongating phase they reach 0.30 μm in the rhizomorphic cortex. Mucilaginous material is progressively deposited around hyphae and in the most differentiated zones, coalesce to fill interhyphal spaces. This extracellular matrix seems to play a role in maintaining cohesiveness of the aggregated organs. The tissue in the process of differentiation is scattered with cells highly enriched in mitochondria and with cells virtually undifferentiated. Accumulation of microfilaments takes place in the differentiating zone localized behind the immersed meristematic apex. These structures might be involved in wall synthesis. Glycogen rosettes accumulate in the vegetative mycelium surrounding the aggregating centers, suggesting the possibility of supplying energy during the differentiating processes. The vacuolar system, represented by autophagic vacuoles which are present until the differentiation phase, presumably may also participate in the biochemical changes that occur during aggregation. Coremial cells are characterized by an increase in wall thickness, a highly sinuous plasma-membrane as well as large amounts of mucilaginous compounds accumulated between hyphae, but in all other respects they resemble the cells of actively growing vegetative hyphae.
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    Protoplasma 116 (1983), S. 125-135 
    ISSN: 1615-6102
    Keywords: Ciliated protozoan ; Sorogenesis ; Extracellular matrix ; Secretion ; Extrusive organelles ; Ultrastructure
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    Topics: Biology
    Notes: Summary Sorogena stoianovitchae Bradbury andOlive is an unusual kinetophragminophoran ciliate characterized by the aggregation of individual trophic cells to form a multicellular sorogen that rises aerially from the medium surface to produce a sorocarp. A light and electron microscope study of sorogenesis revealed how the sorogenic cells produce the stalk. The feeding ciliates contain numerous subpellicular expansions of rough endoplasmic reticulum cisternae that are only one element of the secretory system necessary for stalk formation. Upon aggregation, the stalk secretory system develops further, initially with the proliferation of the subpellicular expansions. Then, during later aggregation and early sorogenesis, other secretory elements appear: stalk material vesicles, dense granule vesicles, and electron lucent vacuoles. These are all derived from the rough endoplasmic reticulum. Only the subpellicular expansions and stalk material vesicles appear to add to the stalk matrix. The dense granule vesicles could be precursors of the stalk material vesicles; the electron lucent vacuoles, which can be demonstrated to contain fibrillar material, are perhaps by-products of the secretory process. Sorogenesis inSorogena occurs by a mechanism unique among the stalk-producing protists that have been studied.
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    Protoplasma 116 (1983), S. 136-144 
    ISSN: 1615-6102
    Keywords: Stalk ; Ciliated protozoan ; Sorogenesis ; Extracellularmatrix ; Ultrastructure ; Secretion
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    Topics: Biology
    Notes: Summary The sorogenic cells of the haptorid ciliateSorogena stoianovitchae Bradbury andOlive secrete a hydrated, fibrillar matrix that becomes organized to form a stalk. This light and electron microscope study examines the role of the secreted stalk matrix in the development of the sorocarp. The stalk material is secreted as a compact clump or clumps of material that expands as it hydrates. The expanded stalk material is initially viscous, but soon, through dehydration or cross-linking, solidifies to form a sheath. The sheath is the outside layer of the stalk, provides structural support, and controls the expansion of the newly secreted stalk material. The sorogenic cells continue to secrete stalk material that expands once it is outside of the cell. The resulting expansion force is directed upward by the sheath, thereby moving the cells further above the surface. The continued dehydration of the sheath material provides an inwardly directed force that also contributes to the upward movement of the sorogen.
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  • 29
    ISSN: 1615-6102
    Keywords: Cytochrome oxidase ; Cytochemistry ; Ultrastructure ; Neurospora ; Heat treatment
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    Notes: Summary Cytochrome oxidase (EC 1.9.3.1) activity of conidia of wild-typeNeurospora crassa was detected cytochemically at the ultrastructural level by 3,′-diaminobenzidine (DAB) reaction during heat treatment at 46 °C and after shift down to 25 °C. At 46 °C the decrease in the enzyme activity was shown to be time-dependent (0–10 hours) in the progressively overswelling conidia. Gradual recovery of the DAB reaction occurred in mitochondria of conidia outgrowing conidiogenous germ tubes (microcycle conidiation) when shifted down to 25 °C. Inactivation of cytochrome oxidase was efficiently prevented during heat treatment in the presence of Tween 20. Such conidia no longer overswelled but still required shift down to 25 °C to produce germ tubes with delayed conidiogenous ability.
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    Protoplasma 117 (1983), S. 7-16 
    ISSN: 1615-6102
    Keywords: Beta vulgaris ; Chloroplast ; Ultrastructure ; Ultraviolet radiation
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    Topics: Biology
    Notes: Summary The effect of UV-C (254 nm) and UV-B (290–320 nm) radiation on leaves ofBeta vulgaris L. at the ultrastructural level was investigated. Although the damage caused by UV-C radiation was more striking than that resulting from UV-B radiation, several structural changes were seen in the UV-B treated material. Generally the effects of UV-B and UV-C radiation were different, suggesting different mechanisms of action, discernible even at the ultrastructural level.
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    Protoplasma 117 (1983), S. 116-129 
    ISSN: 1615-6102
    Keywords: Pollen ; Amoeboid tapetum ; Arum italicum ; Ultrastructure
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    Topics: Biology
    Notes: Summary A tapetum is found around all higher plant meiocytes and is thought to nourish them. It may, in turn, be influenced by their development. The mature tapetal membrane in amoeboid (or periplasmodial) tapeta, of whichArum italicum is an example, fits closely around the developing meiocyte. This description of tapetal ultrastructure starts from the meiotic prophase when the tapetum is still cellular and comprises two rows of cells on the inside of the tetrasporangiate anther. The radial walls of the still cellular tapetum start to dissolve during leptotene of the first meiosis. The lysis begins in the middle lamella in those areas penetrated by the most plasmodesmata. The walls in contact with the meiocytes on the other hand do not disappear until after the first meiosis. At telophase the now fused cytoplasmic tapetal mass begins to extend its joint plasmamembrane, amoeboid fashion, into the loculus. A cluster of microtubules can be seen at the apex of this intrusion apparently initiating or maintaining the shape of the invading plasmamembrane front. The tapetum now adheres closely to the microspores. The tapetal zone closest to the spores has a prominent population of microtubules and just a little ER, whereas the outer zone away from the spores contains all the other organelles. The inner zone, in a squash preparation, is not readily separated from the spores. The microtubules, at the middle microspore stage, are no longer to be seen in a circle around the microspores, but spread out with some lying orthogonal to the now-forming exine surface. In places the tapetal plasmamembrane begins to retract from the exine leaving roughly cone-shaped zones (spines) which become filled with fibrillar material. This material begins to be deposited on the exine surface. These “spines” are both PAS. and Coomassie-blue positive and susceptible to acetolysis.
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  • 32
    ISSN: 1615-6102
    Keywords: Histochemistry ; Sclerotia ; Sclerotial germination ; Sclerotinia ; Ultrastructure
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    Notes: Summary Cytoplasmic reserves and extracellular substances were progressively broken down and utilized during carpogenic germination of sclerotia ofSclerotinia minor. Glycogen, wall polysaccharides and polyphosphate granules were removed first from regions of the sclerotium distant from developing apothecia, while protein bodies near the base of apothecial stipes were hydrolysed before those further away. The number of profiles of mitochondria and endoplasmic reticulum in cortical and medullary hyphae increased at the onset of germination, indicating increased metabolism in the hyphae. In contrast to developing sclerotia, simple pores with Woronin bodies were frequent in walls and septa during germination. Hyphae that appeared to converge towards the base of apothecial initials retained their cytoplasm and organelles until late in germination and hydrolysis of their reserves was delayed; these are interpreted as translocatory hyphae, although further work is required to determine their role unequivocally. When apothecia were fully developed, hyphae throughout the sclerotium were empty and the walls and extracellular matrix of cortical and medullary hyphae had almost completely broken down.
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    Protoplasma 118 (1983), S. 56-70 
    ISSN: 1615-6102
    Keywords: Carposporophyte ; Cell division ; Mitosis ; Rhodophyta ; Ultrastructure
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    Topics: Biology
    Notes: Summary Mitotic ultrastructure was observed in meristematic cells of carposporophyte generations of the freshwater red algaBatrachospermum ectocarpum. Prior to nuclear division, cell elongation occurs resulting in the nucleus being located at the proximal end of the cell and separated by a large central vacuole from a distal “empty region” which the daughter nucleus eventually occupies. In late prophase, nuclear envelope-attached polar rings are situated adjacent to shallow nuclear invaginations. At prometaphase the nuclear invaginations extend deeply into the nucleus forming continuous cytoplasmic channels containing microtubules. Perinuclear ER and a typical equatorial chromosomal plate are formed by metaphase at which time the nuclear envelope lining the cytoplasmic channels has dispersed. Chromosomal and non-chromosomal microtubules converge at the poles where a single, large gap is seen in the otherwise intact nuclear envelope. Polar rings were not detected in the few mitotic cells observed beyond prometaphase but are thought to be present. During anaphase an interzonal midpiece is formed and the distal-most incipient daughter nucleus moves laterally past the central vacuole into the apical “empty region”. Features of mitosis inBatrachospermum are believed to be intermediate between those exhibited by the lower and higher orders of red algae, this being consistent with the taxonomic placement of the genus in theNemaliales, the least advanced order of the classFlorideophyceae.
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    Protoplasma 118 (1983), S. 95-103 
    ISSN: 1615-6102
    Keywords: Nuclear bodies ; Nucleolus ; Ultrastructure
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    Topics: Biology
    Notes: Summary Nuclear bodies are found in interphase nuclei of root apices of a number of plant species. They often show differences in structure and position relative to the nucleolus and this has led to an attempt to define two classes of body. However, in some species their separation into two classes on structural grounds alone breaks down, indicating that although they may occupy different positions within the nucleus they may in these particular cases be only different forms of the same body. The two extremes of the range of bodies examined represent what have been called “nucleolus-associated body” (karyosome) and “dense body”. The nucleolus-associated body is typically attached to, or adjacent to, the nucleolus. It is composed of fibrils 4–8 nm wide and often has an open structure showing compound threads or fibrils separated from each other by electron-lucent spaces. The dense body is more compact in structure and typically lies free in the nucleoplasm. Both types of body have an affinity for silver ions which, together with their staining reaction following treatment with EDTA, indicates that they consist of ribonucleoprotein. The characteristics of nuclear bodies found in different plant species have some relationship with the structure and DNA content of the interphase nucleus. Nucleolus-associated bodies are characteristic of species with an areticulate nuclear structure (2 C DNA content 〈6 pg), while dense bodies are common in species with a reticulate nuclear structure (2 C DNA content 〉6 pg). The possible functions of the two forms of nuclear body are discussed.
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  • 35
    ISSN: 1615-6102
    Keywords: Calcification ; Coccolithophorids ; Polysaccharide localization ; Ultrastructure
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    Topics: Biology
    Notes: Summary Emiliania huxleyi is a marine coccolithophorid which produces coccoliths,i.e., particles consisting of calcite and macromolecular organic material. The coccoliths are formed intracellulary in specialized organelles which comprise a coccolith vesicle (CV) and a reticular body (RB), together forming the CV/RB system or calcifying system. After termination of calcification, the coccolith is extruded and incorporated into the coccosphere,i.e., one or several layers of extracellular coccoliths surrounding the cell. Apart from the coccolith-producing cells (C cells) ofE. huxleyi, there are naked cells (N cells) which seem to have lost the capacity to produce coccoliths but are very similar to the C cells in other morphological respects. Biochemical studies have revealed that polysaccharides may play a regulatory role in calcification. The aim of the present study was to determine the localization of polysaccharides in both C and N cells electron microscopically. For this purpose, a cytochemical staining technique according toThiéry (1967) was applied. The CV/RB system of C cells was conspicuously stained. Due to the excellent stainability of this system, a putative succession of morphological stages during coccolithogenesis could be described. The staining pattern of the N cells closely resembled that of the C cells. It was found, however, that the “calcifying” system of N and C cells differed in both morphology and position. It is suggested that the divergent morphology of the “calcifying” system of N cells accounts for its failure to produce coccoliths.
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  • 36
    ISSN: 1615-6102
    Keywords: Meiosis ; Microtubules ; Polarity ; Ultrastructure ; Mosses
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    Topics: Biology
    Notes: Summary An extensive system of microtubules develops during meiotic prophase in the mossRhynchostegium serrulatum (Hedw.)Jaeg. &Sauerb. Development of the cytoskeleton can be traced to early prophase when the nucleus is acentric and the single plastid divides into four plastids. The cytoskeletal microtubules are associated with equidistant positioning of the four plastids at the distal tetrad poles and with migration of the nucleus to a central position in the sporocyte. The cytoskeleton, which interconnects plastids and encloses the nucleus, contributes to the establishment of moss sporocyte polarity. Just prior to metaphase I evidence of the prophase cytoskeleton is lost as the bipolar metaphase I spindle develops in association with discrete polar organizers located in opposite cleavage furrows between plastids.
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    Protoplasma 113 (1982), S. 10-22 
    ISSN: 1615-6102
    Keywords: Diatom ; Motility ; Mucopolysaccharide ; Secretion ; Staining ; Ultrastructure
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    Topics: Biology
    Notes: Summary Generation of movement in benthic diatoms is thought to be intimately associated with secretion at the raphe, a slit in the silica cell wall. The presence and distribution of extracellular substances and their source was investigated cytochemically by transmission electron microscopy. Extracellular material, possibly-acid mucopolysaccharide, was observed consistently within the entire length of the raphe of both valves and also as a sheath enveloping the silica frustule. Such quantities of extracellular material are absent in conventionally fixed motile diatoms. Numerous cytoplasmic vesicles, with fibrillar contents, distributed peripherally but concentrated along the raphe and at the cell poles, react strongly with a polysaccharide specific stain; their distribution in the cell and polysaccharide content suggest these may be the source of raphe and sheath material. Results support the most recent theories on the mechanism of locomotion in outline only; the details cannot be clarified. Localization procedures using alcian blue and silver staining of peroxidised sections are discussed briefly.
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  • 38
    ISSN: 1615-6102
    Keywords: Fungi ; Gilbertella persicaria ; Membranes ; Mitochondria ; Organelle isolation ; Plasma membrane ; Ultrastructure ; Vacuoles
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    Notes: Summary Methods are described for isolating and identifying subcellular membranes from walled hyphae ofGilbertella persicaria. Differences in thickness and symmetry of membranes and in contents of vesicles were used to distinguish different types of membranes. Mitochondria, vacuoles, plasma membrane, and vesicles with attached ribosomes from homogenized germlings equilibrated at the 1.2/1.4 M interface in discontinuous sucrose gradients. Accelerated flotation in centrifuged Ficol-sucrose gradients resulted in the additional separation of the mixed membranes into three fractions: one contained predominantly intact mitochondria, another was composed of vacuoles and vesicles coated with ribosomes, and a third was enriched in plasma membranes. Based upon morphometric analysis, these fractions contained 92% mitochondria, 53% vacuoles, and 89% plasma membranes, respectively. The source of vesicles coated with ribosomes was investigated since rapidly growing hyphae ofG. persicaria contained little rough endoplasmic reticulum as compared with other classes of membranes. Reconstruction from electron micrographs of mitochondrial fragmentation and vesiculation suggested that most of the ribosome-coated vesicles originated from disrupted mitochondria rather than from rough endoplasmic reticulum. The study demonstrates the utility of ultrastructural markers to identify membranesin vitro independent of, or as an adjunct to, cytochemical and biochemical markers.
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    Protoplasma 112 (1982), S. 26-36 
    ISSN: 1615-6102
    Keywords: Aneilema ; Commelina ; Cytochemistry ; Evolution ; Papillae ; Pollination ; Secretion ; Stigmas ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The stigmas of species inAneilema andCommelina are trifid and comprise elongate papillae. Progressive degeneration of papular cells is observed in stigmas from open flowers and at anthesis papillae may be moribund and collapsed. Fluid emanating from the hollow style flows onto the surface through ruptures in the cuticle at the interpapillar junctions into the interstices at maturity. This secretion stains positively for protein. Stigmas are of the “wet” type. The cuticle overlying the papillar cells is ridged and at the final stages prior to flowering this cuticle becomes detached from the underlying cellulosic wall. The sub-cuticular space so formed is filled with secretion. InAneilema species detachment of cuticle is at the papillar tip and along the lateral walls. InCommelina species the anticlinal walls of adjacent papillae are strongly attached for much of their length and thus detachment of cuticle is restricted to the papillar tip. The cell wall at the tip in both genera may proliferate forming a rudimentary transfer-cell type wall. The secretion is considered to be produced by the papillar cells. It is PAS positive but fails to stain for protein and in both the light and electron microscopes appears heterogenous. Pollen attachment, hydration, germination and early tube growth are very rapid following self-pollination, the pollen tubes entering the neck of the style within ten minutes of attachment. A unique character combination involving pollen and stigmas in these genera indicates a monophyletic origin.
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    Protoplasma 112 (1982), S. 81-91 
    ISSN: 1615-6102
    Keywords: Didymium iridis ; Microcyst-encystment ; Ultrastructure ; Differentiation ; Myxomycete
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    Topics: Biology
    Notes: Summary Myxamoebae ofDidymium iridis were removed from the bacterial food source and induced to encyst by transfer to 10 mM phosphate buffer. After 24 hours of induction approximately 90% of the myxamoebae had differentiated into microcysts. The kinetics of encystment were not significantly affected by pH or osmolarity of the encystment medium. Early stages of encystment were distinguished by the appearance of autophagic vacuoles and an extracellular “slime-like” sheath. The outer wall layer, consisting of dense fibrils, was unevenly deposited after 4 hours. An electron-lucent, second wall layer appeared between 5–10 hours followed by a densely packed, third wall layer adjacent to the plasma membrane. Wall formation appeared to involve smooth-membraned vesicles of possible Golgi origin. The vesicle contents and outer wall layer reacted with the periodic acid-silver methenamine stain for polysaccharide. The density of intramembrane particles of the protoplasmic fracture face increased during encystment with a gradual formation of aggregates of particles.
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  • 41
    ISSN: 1615-6102
    Keywords: Discophrya ; Tentacle contraction ; Cations ; Calcium ; Ultrastructure
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    Topics: Biology
    Notes: Summary Discophrya collini is a suctorian protozoan with contractile tentacles containing a microtubule-lined canal and microfilaments. The effects of a range of cations on tentacle contraction and ultrastructure have been determined. Treatment with 80 mM CaCl2 and 95 mM MgCl2 causes contraction to 28% and 57% of the control length respectively. Re-extension takes over 4 hours in the culture medium, but CaCl2-treated tentacles are re-extended after a 5 minutes treatment with 10−2 M EDTA or 5 × 10−3 M EGTA. CuCl2 causes a significant contraction at 10−5 M (to 77%); LaCl3 at 10−4 M (to 65%); ZnCl2 at 10−2 M (to 65%), but BaCl2, CoCl2, MnCl2, NiCl2, and SrCl2 cause significant changes only at 10−1 M. The cytoplasm of CaCl2-treated cells contains two forms of membraneous structures when viewed in TEM; that of MgCl2-treated cells reveals granular areas of medium electron density. None of these features are seen in control cells. The microtubules of the tentacle canal appear to be intact upon its retraction into the cell with no change occurring in the numbers or relative positions of the microtubules. The tentacle cortex is wrinkled. It is suggested from this and previous work that tentacle contraction may be mediated by a microfilament-based mechanism, and that calcium may be involved.
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  • 42
    ISSN: 1615-6102
    Keywords: Fungus ; Zoospore ; Ultrastructure ; Membranes
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    Topics: Biology
    Notes: Summary Determining how the orientation and association among organelles are maintained within zoospores of theChytridiales is important to understanding the control of zoospore motility. Zoospores of the aquatic fungi,Chytriomyces aureus andC. hyalinus, contain microbody-lipid globule complexes with an elongate microbody adjacent to the portion of a lipid globule facing the cell's interior and a fenestrated cisterna (the rumposome) opposed to the surface of the lipid globule toward the plasma membrane. Mitochondria are intimately associated with the microbody. Electron microscopy of the microbody-lipid globule complex fixed in glutaraldehyde and osmium tetroxide, with or without tannic acid, reveals cross-linking bridges connecting the rumposome to the plasma membrane, to the microbody, and to microtubules of the rootlet extending from the kinetosome. It is concluded that these bridges are responsible, at least in part, for the consistent location of the microbody-lipid globule complex in the zoospore body. The possible role of the rumposome as a receptor organelle is discussed.
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  • 43
    ISSN: 1615-6102
    Keywords: Secretion ; Vesicles ; Ultrastructure ; Cultured cells
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    Topics: Biology
    Notes: Summary Callus-derived suspension cultures of oats dramatically increase the viscosity of the culture media after one month in culture. Colorimetric assays for sugars and protein, as well as measurements of viscosity, suggest that the released material is a long-chain polysaccharide, probably a pectinaceous substance. These cells grow slowly in liquid culture, yet despite their low cell density, they are able to increase the viscosity of the media several fold within seven days after media transfer. Ultrastructural observations show that oat cells have features common to actively-secreting cells; especially evident are numerous dictyosomes with hypertrophied cisternae. Using a combination of filtering and centrifugation techniques we were able to recover large numbers of intact secretory vesicles. The interior of the vesicles stain with periodic acid-silver hexamine, and colormetric analysis of the vesicle pellet for total sugars confirms the presence of polysaccharides in this vesicle fraction. Because of the uniformity of these cells, the high rate of secretion, and the accessability of a large vesicle population, this culture system is'a useful model for studying the secretory process in plant cells.
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    Plant and soil 71 (1983), S. 147-153 
    ISSN: 1573-5036
    Keywords: Cortex ; Endodermis ; Picea sitchensis ; Tilia cordata ; Transfer cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The endodermis of bothTilia cordata andPicae citchensis progressess through 3 characteristic phases of development. These developments are delayed somewhat in the xylem pole endodermis ofT. cordata, while inP. sitchensis 3–5 passage cells are found. The cortex ofT. cordata is characterised by very thick walls, while that ofP. sitchensis is characterised by a thick walled layer just outside the endodermis and by 2–3 outer layers of transfer cells.
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    Hydrobiologia 106 (1983), S. 43-57 
    ISSN: 1573-5117
    Keywords: eutrophication ; paleolimnology ; diatoms ; sediment chemistry ; peatland management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Stratigraphy of diatoms and chemistry in the surface sediment deposited at 35 m depth in Lake Polvijärvi was studied. The existence of annual laminations or varves in the sediment allowed a precise dating of the profile. Diatoms were analysed in 0.5 cm sequences; from 0 to 16.0 cm continuously and then intermittently every fourth 0.5 cm down to 44.0 cm. Sediment chemistry (loss-on-ignition, C, N, Fe, Mn, Mg, P, chlorophyll and carotenoids) was analysed from sediment surface down to 10.5 cm of altogether 33 subsamples, each containing 1–3 varves, and spanning the period 1921–1980. From 4.5 cm depth upwards the diatom concentration strongly increases, and the plankton diatom succession from Tabellaria flocculosa through Asterionella formosa to Melosira ambigua and Fragilaria crotonensis reflects a marked eutrophication of the lake. This algal succession occurs in pace with an increase in sediment accumulation rate and changes in sediment chemistry, which indicate increased allochthonous inputs and enhanced algal production in the lake. The change of the lake ecosystem is contemporaneous with extensive peatland draining and fertilizing that was carried out on its watershed during the past two decades. Existing chemical data from a number of lakes situated within the drainage area prove that at present the treated peatlands are the main source of nutrient loading of Lake Polvijärvi. A former period with indications of slightly increased productivity of the lake was dated by varve counting to AD 1690–1910 (35–12 cm). This period (characterised by Asterionella formosa) may coincide with that of the slash-and-burn cultivation in the area.
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    Hydrobiologia 103 (1983), S. 45-51 
    ISSN: 1573-5117
    Keywords: paleolimnology ; magnetism ; dating ; Australia ; North America ; Japan ; Europe ; Near East
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Paleolimnomagnetic records from five regions of the world have been combined with historical magnetic field observations in order to produce regional geomagnetic master curves.
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    Hydrobiologia 103 (1983), S. 141-146 
    ISSN: 1573-5117
    Keywords: paleolimnology ; lakes ; sediments ; eutrophication ; Cladocera ; Switzerland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Species composition and density of cladoceran populations changed in Lakes Zürich, St. Mortiz and Baldegg as human populations increased in these watersheds. Lake Zürich sediments became annually laminated in the 1890's as a result of increased organic input as the size of the cities surrounding the lake grew. At the same time, the Bosmina species changed from a oligotrophic form (longispina) to a eutrophic form (longirostris). An increase in Daphnia spp. populations also occurred at this time in the lake's history. Bosmina longispina reappeared in the lake in 1965 as the lake's trophic status changed from eutrophic to mesotrophic due to effective sewage treatment facilities. Annual laminations appear in the Lake St. Moritz sediments about 1910. Shortly thereafter, a shift from B. longispina to B. longirostris occurred. This change in trophic status is associated with increased tourism in the area. Lake Baldegg sediments also show annual laminations beginning in 1885 and a similar shift in the Bosmina species. Other cladoceran remains were too scarce to be useful in interpreting the histories of these lakes.
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    Hydrobiologia 103 (1983), S. 37-44 
    ISSN: 1573-5117
    Keywords: paleolimnology ; magnetic minerals ; sediment sources
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This review outlines the origin and environmental significance of magnetic minerals in lake sediments. Attention is drawn to situations where the patterns of mineral magnetic variation is a reflection of processes other than changing erosion rates and fire incidence. The use of mineral magnetic techniques in sediment source tracing, palaeoclimatic studies and the reconstruction of particulate pollution history is illustrated by means of case studies from Britain and N. America. The value of magnetic susceptibility as an on-site core logging technique is shown by reference to data from Lake Washington.
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    Hydrobiologia 103 (1983), S. 53-58 
    ISSN: 1573-5117
    Keywords: paleolimnology ; secondary ferrimagnetic oxides ; S.I.R.M. ; magnetic susceptibility ; laminated sediments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The magnetic parameters S.I.R.M. and magnetic susceptibility have been used to try and establish the fire histories of lake drainage basins. The technique is demonstrated using sediments from three lakes: Llyn Bychan (N. Wales), a lake with a recently burnt catchment, Lake Biscarrosse (S. W. France), a lake with a well documented fire history, and Lake Laukunlampi (E. Finland), a lake with laminated sediments and a long, but unknown fire history.
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    Hydrobiologia 103 (1983), S. 59-64 
    ISSN: 1573-5117
    Keywords: paleolimnology ; sedimentation pattern ; magnetic susceptibility ; Scanian lakes ; palaeomagnetism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Magnetic susceptibility measurements have been used to correlate synchronous depths in ca. 50 l m. cores of recent sediment from a small lake (area ca. 55 ha) in Scania, southern Sweden. The three-dimensional picture of sediment accumulation which emerges provides a basis for studying sediment deposition patterns through time. Using a palaeomagnetic chronology, the results show that the pattern and rate of accumulation have dramatically altered during the past 350 years, thus making semiquantitative studies of downcore sediment properties in a single core problematical. Possible reasons for these phenomena are briefly discussed.
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    Hydrobiologia 103 (1983), S. 65-69 
    ISSN: 1573-5117
    Keywords: paleolimnology ; X-ray radiography of sediment cores ; sedimentary properties ; sediment accumulation
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    Topics: Biology
    Notes: Abstract X-ray radiographs of unextruded sediment cores are used for the documentation and the interpretation of primary and secondary sedimentary structures, and for the correlation of synchronous laminations between different cores. The vertical variation in bulk density, water content and void ratio, and the increase of solids with increasing sediment depth are calculated from the recorded film density along the radiographs. The X-ray radiographic technique for the study of sedimentary properties is fast and non-destructive. The technique is especially valuable when studying the uppermost part of the sediment cover and the processes of sediment redistribution and sediment accumulation. Studies are being undertaken in several Swedish lakes and coastal bays, where mono- and stereoradiographs are being used to classify bottom and sediment types, to interpret changes in the depositional environment, and to calculate past and present rates of sediment accumulation.
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  • 52
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    Keywords: paleolimnology ; sediment core positioning ; hydrographic survey ; computer contouring ; morphometric calculation
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    Notes: Abstract Automated techniques for sediment core site positioning, hydrographic survey, and contour mapping are described. The computer contouring system can be extended to calculate values for morphometric variables such as maximum length (1max), maximum width (wmax), lake perimeter (10), lake area (A) and lake volume (V). The techniques are exemplified using results from Augher Lough, Northern Ireland.
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    Hydrobiologia 103 (1983), S. 75-79 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Dead Sea climate ; pore-waters ; diffusion ; sediments ; salinity ; porosity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The salinity of lakes is subject to variations imposed by climatic changes. These variations are recorded in the salinity profile of pore waters. Meromictic lakes, such as the Dead Sea, are a special case where waters which underlie the mixolimnion reflect salinity variations. In a sediment core from Dead Sea shallow waters, the salinity profile exhibited a minimum at about 1.9 m depth. It is shown by a diffusion model that this minimum can be attributed to lower salinities which prevailed at the sediment water interface for several decades around the turn of this century. No such minimum was observed in a sediment core from the deepest part of the lake where, during the last two centuries, the overlying brines had a constant salinity.
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    Hydrobiologia 103 (1983), S. 81-84 
    ISSN: 1573-5117
    Keywords: paleolimnology ; geochemistry ; trace elements
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    Topics: Biology
    Notes: Abstract A geochemical study of lacustrine sediments was undertaken as part of a major paleolimnological project concerned with the impact of man on lakes. Factor analysis was applied to the geochemical data obtained from a core from one lake. Three principal factors which explained most of the variance of the initial data were identified. Factor I is related to the organic:mineral component ratio of the sediment, and can be considered as an indirect index of change in lake trophic status through time. Factor II is related to the granulometric composition of the sediment and matches evidence for changes in the hydrological regime of the lake. Factor III reflects changes in redox potential and is more closely related to processes occurring within the lake than other factors. Specific geochemical associations of elements are connected with each factor and are the factor indicators. The correlation of these associations are analyzed as geochemical indices of the variability of sediment accumulation conditions in time.
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    Hydrobiologia 103 (1983), S. 91-97 
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatoms ; assemblage indicators ; Finland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diatom assemblages from the topmost sediment of 49 lakes were ordinated by means of a program for detrended correspondence analysis and reciprocal averaging (Decorana). Five lake groups were separated, each having more or less characteristic diatom assemblages and water quality. Shifts from one group to another caused by factors such as acidification, artificial drainage, diffuse nutrient loading and sewage are discussed.
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    Hydrobiologia 103 (1983), S. 85-90 
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatoms ; East Africa ; transfer functions ; pH-indicators
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    Topics: Biology
    Notes: Abstract Our purpose is to establish the quantitative relationship between recent diatom floras and ecological parameters, in order to extrapolate the results to the past. The parameter pH is here considered as an example. This work is based on the study of about I60 diatom samples from East Africa and of their corresponding biotopes. We propose some statistical methods to interpret the data. Correspondence analysis allows us to define the pH-indicator species. The regression calculations allow pH values to be calculated using the percentage of the diatom species in a sample.
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    Hydrobiologia 103 (1983), S. 99-102 
    ISSN: 1573-5117
    Keywords: paleolimnology ; organic matter ; bacteria ; aerobic decomposition ; oxygen consumption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The investigations were carried out in lakes situated in the forest zone (Karelian Isthmus, Baltic zone, South Ural) and in the forest-steppe (South Ural) of the USSR. The lakes differ in their bioproductivity and in the intensity of their human influence. The amount of organic matter accumulating in the sediments is closely related to production and decomposition processes in the trophogenic layer. Processes of organic matter transformation were found to be most active in the uppermost sediment. The quantity of bacteria shows no correlation with the organic matter content of the sediment. Increase in organic matter, up to 70–80% of dry weight, is often accompanied by a decrease in bottom bacteria. The intensity of aerobic decomposition of the labile organic matter can be judged from the oxygen demand of the sediments. However, it is important to differentiate between chemical and biological oxidation processes. The quantity of bottom bacteria correlates closely with the value of oxygen consumption only in cases of high sediment redox potentials.
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    Keywords: paleolimnology ; sedimentary pigments ; primary production ; core analyses
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    Notes: Abstract A statistically significant correlation of the type y = axb between sedimentary plant pigments and contemporary algal primary production has been found in a study of the recent trophic evolution of twelve Italian lakes. The equation is used to assess baseline production levels in periods (50–70 years ago) when human influence was low.
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    Hydrobiologia 103 (1983), S. 107-111 
    ISSN: 1573-5117
    Keywords: paleolimnology ; lake ontogeny ; lake succession ; eutrophication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Paleolimnologists frequently invoke the term ‘eutrophication’ for describing apparent enrichment phases in the history of a lake. I argue that this term is often used incorrectly and that alternative explanations can serve as more accurate descriptions. Increased organic content in the sedimentary record may result from increased nutrient availability (eutrophication), but it can also reflect decreased residence time of water, or changes in biotic interactions, or changes in lake morphometry. Additionally, I argue that ‘eutrophication’ is an inappropriate term for describing the aging process of lakes. Lake ontogeny is the preferred term, as it does not imply directional changes in nutrients, nor in community structure.
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    Keywords: paleolimnology ; lakes ; eutrophication
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    Notes: Abstract Problems relating to the disappearance and eutrophication of lakes as a result of human activity are presented. A study area was selected located in an agricultural/forest region where human influence on lakes used to be minimal. In the past 25 years a number of lakes have completely disappeared and others have become rapidly enriched. Human interference has included: the pulling down of water mills, drainage and river canalisation, construction of holiday resorts, development of intensive farming, increased mineral fertilization, and the extension of roads and road traffic.
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    Hydrobiologia 103 (1983), S. 177-179 
    ISSN: 1573-5117
    Keywords: paleolimnology ; sediment chemistry ; heavy metals ; ATP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This investigation is concerned with the impact of industrial and municipal waste discharge on lakes near the city of Tampere, Finland. The record of P, Zn and ATP in the recent sediments of Vanajavesi I, a polluted lake, and Mallasvesi, a largely unpolluted lake, are compared.
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    Hydrobiologia 103 (1983), S. 169-175 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Chironomidae ; subfossil remains ; shallow lake ; eutrophication ; Lake Balaton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lake Balaton, the largest shallow lake in Central Europe, is about 20 000 years old. An enormous increase in tourism and the disproportionate building development of the last few decades has resulted in the acceleration of eutrophication in the lake. Widespread research to reveal the causes of water-quality deterioration and possible ways of protection against it have recently started. The investigation of the larvae of non-biting midges (Diptera: Chironomidae) in the sediment of the open-water zone has also begun. The contemporary faunal composition strongly correlates with the trophic gradient along the longitudinal axis of the lake. We therefore supposed that the eutrophication process should be identifiable from the analysis of subfossil chironomid head capsules from the upper (15 cm thick) layer of the sediment. We found that quantitative results could only be obtained when fragments as well as relatively intact head capsules are considered. Our data verify that the originally oligo-mesotrophic community has been gradually replaced by eutrophic species in a west to east direction. Large-bodied larvae belonging to the Chironomus plumosus group mix the sediment down to 15 cm as they build their tubes and consequently alter the original proportions of head capsules at the different levels. So the sequence of communities through the sediment-layers is not quite reliable.
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    Hydrobiologia 103 (1983), S. 181-184 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Clostridium perfringens ; sewage pollution ; eutrophication
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    Topics: Biology
    Notes: Abstract The horizontal and vertical distribution of the gram-positive, non-motile, spore-forming and rod-shaped bacterium Clostridium perfringens Holland was studied. The aim of the study was to estimate the quantity of C. perfringens at different depths of the sediment and evaluate the effect of human effluent which the lake received between 1940 and 1956. C. perfringens lives in the colon of man. Because it is spore forming and cannot multiply under a temperature of 20 °C and, according to the studies of Seppänen et al. (1979) it can be at least 300 years old, it may be a suitable paleolimnological indicator of pollution by human effluent. The results showed that the amounts of Clostridium increased at the same level where redox potential decreased in the sediment due to the beginning of effluent disposal at a depth of 40 mm. The maximum number of Clostridium colonies occurred between 0–30 mm depth.
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    Hydrobiologia 103 (1983), S. 193-198 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Lake Michigan ; geochemistry ; stratigraphy ; Quaternary ; trace elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The stresses placed on Lake Michigan since the advent of industrialization require knowledge of the sedimentology of the whole lake in order to make informed decisions for environmental planning. Sediment accumulation rates are low: areas of the lake receiving the most sediment average only 1 mm a−1; deep-water basins average 0.1 to 0.5 mm a−1; and large areas are not receiving any sediment. Sediment was deposited rapidly (typically 5 mm a−1), in the form of rock flour, during the deglaciation of both Lake Michigan and Lake Superior Basins. Then the rate of accumulation decreased by 80–90% and has remained relatively constant since final deglaciation. Because active sedimentation occurs mostly in the deep water areas of the lake, the sediment remains undisturbed and contains a record of the chemical history of the lake.
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  • 65
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    Keywords: paleolimnology ; annual laminations ; magnetism ; sediment influx ; sediment chemistry ; eutrophication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sediments of Loe Pool, a eutrophic coastal lake in south west England, consist largely of laminated clays and clay-gyttjas. Studies of the diatom microstratigraphy of frozen sediment cores from the Pool indicate that the laminations are annual, and that they contain pairs of light and dark bands formed by seasonal variations in the supply of sediment to the Pool from its catchment. Analysis of the magnetic properties of individual laminations demonstrates the presence of physical and mineralogical microstratigraphic variations, which may also be related to seasonality. A varve chronology, which is confirmed by 137Cs analysis and historical records, has been used to provide a timescale for the interpretation of data from other paleolimnological studies. A close agreement between variations in the abundance of sedimentary Sn, and the history of mining in the catchment, has been found. Similarly, analysis of total organic matter, total phosphorus, sedimentary chlorophyll a, sterols, diatoms and Cladocera in the uppermost sediments all indicate eutrophication of the Pool in the period AD 1940 to the present.
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    Hydrobiologia 103 (1983), S. 205-210 
    ISSN: 1573-5117
    Keywords: paleolimnology ; colluvium ; soil chemistry ; clay ; phosphorus loading ; tropical lakes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The long-term impact of Maya culture on a lowland tropical watershed is assessed, using data from a 9.2 m sediment core taken from deep water (28 m) in Lake Quexil. Human population growth, estimated by the 1980 archaeological survey, is associated with a shift in the composition of the sediment to a dominance by inorganic material, the Maya clay formation, beginning ca. 3500 B.P. Increasing settlement densities are correlated with accelerated influxes of phosphorus, carbonates, and siliceous sediment. However, chemical data do not track short-term population fluctuations closely. Because much of the sediment is delivered as colluvium, and not by running water, there is a lag between terrestrial disturbance and impact on the aquatic system. As an indication of this lag, contemporary high sedimentation rates are a residual of Maya activity that virtually ceased some 300–400 years B.P. Comparison of the deep-water core with a shallow-water (7 m) section, based on palynological correlation, reveals only minor differences in proximate chemical composition. Chemical influxes are much higher at the deep-water site, however, as a consequence of sediment focusing in this hyperconical basin. Chemical analyses of soil samples from 21 test pits in the Quexil basin support the principal conclusion that bulk soil movement was the mode of nutrient transfer to the lake, following forest clearance by the Maya.
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    Hydrobiologia 103 (1983), S. 199-203 
    ISSN: 1573-5117
    Keywords: paleolimnology ; clay ; cultural disturbance ; erosion ; Guatemala ; Mayan archaeology ; silt ; particle-size analysis ; tropical lakes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Long-term changes of sedimentary particle-size distribution in two tropical lowland lakes were compared with changes of human population sizes, estimated archaeologically, in the drainage basins. Mean particle size of silt and clay fractions (〈64 µm) varied between 3 and 15 µm. High positive skewness and kurtosis of the distributions were associated with smaller particle sizes; hence small mean size resulted from greater influx of small particles while influx of larger particles was probably constant. An inverse correlation between mean particle size and human population size is interpreted to mean that disturbance-induced erosion results in delivery of very fine inorganic particles at higher rates. Within any one basin, particle-size stratigraphy is more precisely related to archaeological time periods than is pollen stratigraphy. An absolute chronology still eludes us, owing to the failure of 14C dating of calcareous, colluvial sediments, but our relative chronology is now more precise than before. If certain assumptions about past hydrologic relations can be met, particle-size analysis is a way of comparing the histories of geographically very different lakes, including lakes from tropical, temperate, and arctic regions.
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    Hydrobiologia 98 (1983), S. 223-235 
    ISSN: 1573-5117
    Keywords: diatoms ; paleolimnology ; lakes ; Minnesota ; sediments ; microfossils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fossil diatom assemblages from the sediment/water interface in 105 Minnesota lakes were compared with measurements of alkalinity, sulphate, total phosphorus, transparency, and water depth at the sample site. Similar assemblages were placed together using cluster analysis and comparisons of environmental variables between diatom clusters were made using an analysis of variance. Total alkalinity and transparency showed the greatest difference among clusters. Samples from shallow eutrophic prairie lakes were dominated by Melosira granulata, Stephanodiscus niagarae and, occasionally, by Stephanodiscus hantzschii. Deep oligotrophic lakes had modest percentages of Cyclotella comta. Dilute acid lakes were dominated either by Melosira distans and M. italica or by Tabellaria fenestrata, Cyclotella stelligera, and in some cases C. glomerata. Assemblages with Cyclotella glomerata and Synedra nana were found in naturally meromictic lakes. Stephanodiscus hantzschii showed a preference for extremely eutrophic lakes. The relationships between recently deposited diatom assemblages and the lake environmental conditions studied here can be used to evaluate the extent of past environmental change in lakes.
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  • 69
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    Keywords: Chrysophycea ; cultural eutrophication ; paleolimnology ; resting cyst ; statospore ; stratigraphy
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    Topics: Biology
    Notes: Abstract The recent sediments of Frains Lake, Michigan contain a rich and well preserved association of chrysophycean cysts. Forty one forms are revealed by scanning electron microscopy (SEM) and light microscopy (LM). Taxonomic descriptions. and SEM micrographs are provided for the dominant forms. The three dominant taxa throughout the sediments, Cysta minima, C. modica and C. subbavaricum, do not show significant shifts in proportional abundance associated with European settlement and the onset of cultural eutrophication. However, certain subdominant taxa do show clear trends. Density counts indicate a dramatic decline in cyst concentration (by volume and by dry mass) and a sharp increase in absolute accumulation (net annual influx) following settlement. The Frains Lake profile of chrysophycean cysts is compared to sequences of other North American and European temperate lakes. The utility of chrysophycean cysts as paleoenvironmental indicators is considered on the basis of these results.
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    Hydrobiologia 103 (1983), S. 125-130 
    ISSN: 1573-5117
    Keywords: paleolimnology ; pollen ; diatoms ; Synuraceae ; Chrysophyta ; meromixis ; eutrophication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The recent history of Little Round Lake, a small meromictic lake in southeastern Ontario, is considered. Pollen analyses were used to identify past changes in terrestrial vegetation, whilst limnological conditions were interpreted on the basis of diatom and chrysophyte microfossils. Contemporaneous with the arrival of European settlers (ca. A. D. 1850), the predisturbance assemblage of oligotrophic Cyclotella diatoms was replaced by Synedra spp., which then succeeded to a eutrophic flora dominated by Stephanodiscus hantzschii. Meanwhile, synuracean algae were almost completely excluded. Over the last 30 years, the algal microfossils indicate that the lake underwent a marked return to oligotrophy. Evidence is presented which suggests that this shift was related to the cultural enhancement of meromixis by the seepage of road salt.
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  • 71
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    Keywords: paleolimnology ; acidic precipitation ; lake acidification ; heavy metal pollution ; diatoms ; cladocerans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sediment cores from nine lakes in southern Norway (N) and six in northern New England (NE) were dated by 137Cs, 210Pb and in NE also by pollen, and were analyzed geochemically and for diatoms. Cores from two N and three NE lakes were analyzed for cladocerans. 137Cs dating is unreliable in these lakes, probably due to mobility of Cs in the sediment. In Holmvatn sediment, an up-core increase in Fe, starting ca. 1900, correlates with geochemical indications of decreasing mechanical erosion of soils. Diatoms indicate a lake acidification starting in the 1920's. We propose that soil Fe was mobilized and runoff acidified by acidic precipitation and/or by soil acidification resulting from vegetational succession following reduced grazing. Even minor land use changes or disturbances in lake watersheds introduce ambiguity to the sedimentary evidence relating to atmospheric influences. Diatom counts from surface sediments in 36 N and 31 NE lakes were regressed against contemporary water pH to obtain coefficients for computing past pH from subsurface counts. Computed decreases of 0.3–0.8 pH units start between I890 and I930 in N lakes already acidic (pH 5.0–5.5) before the decrease. These and lesser decreases in other lakes start decades to over a century after the first sedimentary indications of atmospheric heavy metal pollution. It is proposed that the acidification of precipitation accompanied the metal pollution. The delays in lake acidification may be due to buffering by the lakes and watersheds. The magnitude of acidification and heavy metal loading of the lakes parallels air pollution gradients. Shift in cladoceran remains are contemporary with acidification, preceding elimination of fishes.
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    Hydrobiologia 103 (1983), S. 131-133 
    ISSN: 1573-5117
    Keywords: paleolimnology ; recent sediments ; diatoms ; chrysophytes ; 210lead
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diatom and chrysophyte remains in recent sediments have been examined. The upper stratigraphy is dated by reference to phytoplankton records whilst the lower depends on dates from 210Pb analysis, which shows some unexplained variation. Recent changes, due to farming and domestic drainage, form part of the long term response of the lake to events upon the catchment.
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    Hydrobiologia 103 (1983), S. 135-139 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Traunsee ; artificial meromixis ; ostracods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Traunsee is the deepest (189 m) lake in Austria and has been holomictic until the beginning of industrial discharges from the alkali works in Ebensee. The input of soluble compounds such as CaCl2 and NaCl have produced meromictic conditions. The relatively high oxygen content of the monimolimnion is due to water movements promoted by the large inflow and wind-action. The industrial discharges have caused both meromictic conditions and the deposition of highly alkaline sediment in the southern portion of the profundal zone. The effect of the discharges on the benthic fauna was evaluated by comparing the subfossil benthic fauna of four cores taken inside and outside the affected area. The ostracod fauna resembles that of other large lakes in the Salzkammergut, but also includes groundwater species such as Kovalevskiella and Cypridopsis subterranea. Leucocythere mirabilis has been found for the first time in an Austrian lake. Ostracods and other benthic organisms such as Testacea were not apparently influenced by the meromictic condition. They are, however, missing in all areas covered by the alkaline sludge.
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  • 74
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatoms ; salinity ecology ; annual lamination ; sulphide mud ; meromixis ; Baltic
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    Notes: Abstract Diatoms have been studied in an annually laminated sulphide mud which was deposited after the impoundment of the sea-bay of Gennarbyviken. It was possible to follow how the measured decrease in salinity in the basin was reflected by the diatom flora of the sediment which had been deposited concurrently
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    Hydrobiologia 103 (1983), S. 153-158 
    ISSN: 1573-5117
    Keywords: paleolimnology ; eutrophication ; reservoirs ; Bosmina ; Chironomidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eutrophication of reservoirs can be detected by changes in the abundance of insect and crustacean remains in the sediments. In recently constructed reservoirs, the time of impoundment can be determined through the presence of chironomid head capsules. The initial phase is characterised by highly enriched water as can be seen by the abundance of Bosmina remains. If further nutrient input into the reservoir remains low, the upper part of the sediment column is almost completely lacking in invertebrate remains.
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    Hydrobiologia 103 (1983), S. 159-163 
    ISSN: 1573-5117
    Keywords: paleolimnology ; marine/brackish-freshwater ; transition basin ; wildfowl ; gregarious waterfowl ; allochthonous nutrients ; seasonal eutrophication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new and very special course of events starts in a freshwater basin, just isolated from the sea. In NW Europe this is mainly caused by land uplift in Holocene times or by regression of the sea. It has been thoroughly documented by the gradual change in the composition of the fossil diatom floras. Attention is drawn to the possible impact of migrating birds, especially wildfowl, breeding, resting and moulting in those often shallow, sheltered lakes along the Scandinavian coastlines. The gregarious wildfowl species might seasonally increase the allochthonous input of nutrients in the lake ecosystems and cause marked changes in biotic interactions. A working hypothesis is suggested, that the wildfowl species in question were appreciably more numerous before the arrival of man. Consequently, their effect on seasonal eutrophication cannot be neglected in the paleolimnological context.
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    Hydrobiologia 103 (1983), S. 217-224 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Greenland ; Holocene ; oligotrophication ; pollen analysis ; macrofossils ; arctic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During the Holocene most West Greenland lakes passed from an early eutrophic stage, rich in both flora and fauna, through a mesotrophic to an oligotrophic stage with very low productivity. Temperature conditions were limiting factors only in the very beginning, whereas chemical factors alone were decisive later on.
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    Hydrobiologia 103 (1983), S. 211-216 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Pleistocene aridity ; calcite ; dolomite ; gypsum ; laminated gyttja
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The transition from an arid, glacial Late Pleistocene to an early Holocene (Gamblian ‘pluvial’) moist period has not been documented in Central America. Finding accessible volcanic lakes too youthful, and knowing that most Florida and Yucatan lakes were dry during glacial ages, we sought appropriate deposits lying deeper than 40 m in the deeper karst lakes of the Peten, in northern Guatemala. The bottom half of a 19.7 m core from Lake Quexil, and the bottom third of a 15 m core from Lake Salpeten, appear to be of Pleistocene age. The sediments contain lacustrine shells, sponge spicules, and Pinus pollen, and include several bands of humified gyttja with fragments of wood, but are dominantly montmorillonitic and mixed-layer clays and may be in part colluvial, like the later Holocene Maya clay. Calcite, gypsum, and (in presently saline Lake Salpeten only) dolomite indicate shallow, closed, moderately saline lakes 30–40 m lower than at present. In both cores a layer of inorganic sediment with gypsum dominant, perhaps recording the most arid phase of the glacial Late Pleistocene, overlies a similar clay layer with calcite dominant. As calcite and dolomite occur throughout the section(s), both minerals are believed to be detrital, but one source of calcite is algal crusts, formed and exposed today in the littoral and supralittoral zones. The early Holocene rise of lake levels formed several meters of fossiliferous gyttja with pollen of mesic tropical forest, now assigned to the pan-tropical Gamblian moist episode. Where deposited in oligomictic or meromictic lakes 〉 30 m deep, Gamblian gyttja of pollen zone Pl is finely laminated, the dark layers being richer in Ptot and Stot and poorer in Fe, Mn, Mg, and K than the light (clay) layers, but we cannot yet say that the laminae are annual.
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    Hydrobiologia 103 (1983), S. 225-230 
    ISSN: 1573-5117
    Keywords: paleolimnology ; pollen analysis ; sediment accumulation rate ; vegetation history ; Holstein ; Postglacial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pollen analysis was carried out on the sediments of 10 lakes in eastern Holstein. Sediment accumulation rates varied considerably both from lake to lake and also between respective periods. Increases in sediment accumulation rate often coincide with the Neolithic settlement periods. Faunal remains have been examined from two of these lakes (cf. Günther 1983; Hofmann 1983).
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    Hydrobiologia 103 (1983), S. 231-234 
    ISSN: 1573-5117
    Keywords: paleolimnology ; lake development ; trophic status ; Chironomidae ; Cladocera ; Ostracoda
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microfossil analysis was carried out on 90 samples of a sediment core from Grossensee. The diversity of subfossil Cladocera, Chironomidae, Chaoborus, and Ostracoda reflects at least five different stages of development. Three extended cycles correspond to different climatic periods in the first 10 000 years of the lake's history, while during the last 2 000 years, two shorter cycles can be identified which correlate with proofs or even historical events for human activity in the catchment area. During this time the profundal fauna changed from stenoxibiontic to euryoxibiontic species. In the planktonic fauna a succession of three Eubosmina types, B. longispina, B. coregoni kessleri, and B. coregoni coregoni, was observed. The chydorid and ostracod assembly of the littoral showed no clear change.
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    Hydrobiologia 103 (1983), S. 235-239 
    ISSN: 1573-5117
    Keywords: paleolimnology ; cladoceran analysis ; chironomid analysis ; successions ; lake development
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    Topics: Biology
    Notes: Abstract In the subfossil assemblages of chironomids and cladocerans significant changes were found during the late-glacial and postglacial development of a small, shallow North German lake. The successions observed were obviously not related to eutrophication but to factors such as climatic conditions and lake siltation. The absence of species of the subgenus Eubosmina during postglacial times as well as the predominance of Alonella species among the chydorids was due to the small dimensions of the water body.
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    Hydrobiologia 103 (1983), S. 247-250 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Pannonian lakes ; infilling process ; environmental change ; hydrosere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Data on the stratigraphy and development of a former lake near the town of Vracov, SE Moravia, Czechoslovakia, are presented. The infilling process and vegetation succession are described. An attempt to reconstruct changes in habitat conditions during the last ca. 13 000 years has been made.
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    Hydrobiologia 103 (1983), S. 241-245 
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatom succession ; lake development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Late-glacial and Postglacial sediments of the former Komořany Lake in North-West Bohemia were studied by means of diatom analysis to trace the pattern of past environmental change in the lake. Several phases of lake development could be distinguished. Originally the Komořany Lake was a eutrophic shallow water basin. The diatom succession in the early Postglacial sediment indicates alternating alkaliphilic, epiphytic and planktonic diatom communities associated with changes in water level in the basin. At the beginning of the Subatlantic period the water level began to rise and the development of planktonic diatoms, typical of small eutrophic lakes, occurred. Later, the basin became shallow again and the number of indifferent and acidophilous diatoms increased. This is typical for the final phase of the infilling process of a lake before its transformation to peatland
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  • 84
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatom flora ; sediments ; Vistula Lagoon ; Poland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The variability of Actinocyclus kuetzingii (A.S.) Sim. in the Littorina deposits of the Vistula Lagoon and Lake Druzno were studied. During the time of the Littorina Lake Druzno was a part of the Vistula Lagoon. In the different parts of the Lagoon both the abundance of A. kuetzingii and the diameter of its valves change in a manner associated with phases of the Littorina transgression. Coscinodiscus (Actinocyclus) kuetzingii fo. subsalsa Brockmann is probably an ecotype of A. kuetzingii (A.S.) Sim.
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    Hydrobiologia 103 (1983), S. 257-260 
    ISSN: 1573-5117
    Keywords: paleolimnology ; palynology ; bacteriology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There are many lake basins in the Soviet Union, both in the humid and the arid regions, including lowland and alpine, and shallow and deep-water lakes. Lithological, geochemical, radiological and paleobiological methods have been utilized in studying the history of these lakes. Lake Zaisan in Kazakhstan is described as an example of lake development in the arid zone, whilst the lake systems of the Pribaltica, where human influence on the environment is clearly recorded, exemplifies the humid zone.
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    Hydrobiologia 103 (1983), S. 261-263 
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatoms ; eutrophication ; Ladoga
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Combined paleolimnological investigations of a 1.8 m sediment core from the deepest north-western part of Lake Ladoga show stratigraphic changes in granulometric and chemical composition, organic matter content, diatom species composition and chlorophyll ‘a’ concentration. The sediment accumulation rate was calculated and 5 stages of lake history over the last 4 000 years were described. Changes in lake environment were mainly caused by changes in climate and lake water balance. Human impact on the lake was also traced.
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    Hydrobiologia 103 (1983), S. 265-268 
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatoms ; plankton ; eutrophication ; sediment accumulation rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Species composition, quantity and distribution of diatoms in both the plankton and the surface sediments (0–30 cm) of mesotrophic Lake Krasnoye and eutrophic Lake Vishnevskoye (Karelian Isthmus) were studied. In the mesotrophic lake the composition of dominant diatoms (mainly Melosira) corresponded to those in the plankton. The Araphidineae/Centrales (A/C) ratio was 0.2–0.3%, increasing in the upper layers to 7%. Alkaliphilous and alkalibiontic forms constituted 60% of the total. In the eutrophic lake diatoms with thin valves (mainly Synedra) predominated in the plankton but their quantity in the sediments was insignificant in comparison with other plankton. Nevertheless, the A/C ratio was much higher, 17–35%. Alkaliphilous and alkalibiontic forms accounted for 78–90% of the total number of valves. In both lakes the highest number of diatom valves was registered in the upper layer of the sediments. From the ratio of the total number of diatoms in the upper 5 cm layer to their annual flux to the sediment from the plankton the approximate sediment accumulation rate was calculated to be 1.9 mm a−1 for the mesotrophic lake and 2.5 mm a−1 for the eutrophic one.
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    Hydrobiologia 103 (1983), S. 269-273 
    ISSN: 1573-5117
    Keywords: paleolimnology ; palynology ; pollen diagram ; Holocene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the last decade palynological investigations of the sediments of large lakes in the USSR (Ladoga, Onega, Kubenskoye, Vozhe, Lacha, Beloye) and the Baltic Sea have been carried out. Limnic and marine sediments are notable for their high pollen and spore concentrations and pollen diagrams from these deposits have a regional significance. The diagrams from Lakes Onega and Ladoga belong to the Kola-Karelian type while the sediments of Lacha, Vozhe, Kubenskoye and Beloye belong to the North-Russian type (Neustadt 1957). Three types of Holocene pollen diagrams are registered for the Baltic Sea. Modern and fossil pollen spectra reflect the composition of the zonal vegetation. Late-glacial sediments are found in several profiles of lake and sea deposits. Holocene deposits are divided into eight stratigraphic zones: Late-glacial sediments (DR2-Al-DR3) and Post-glacial sediments (PB, BO, AT, SB, SA). The zonations of lake and sea sediments can be clearly correlated.
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    Hydrobiologia 103 (1983), S. 275-279 
    ISSN: 1573-5117
    Keywords: paleolimnology ; freshwater diatoms ; Middle-Valdaj
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper presents results of diatom analyses of dated Upper-Pleistocene lacustrine deposits from the Shapkina river in the Bolshezemelskaja tundra of the USSR. Both diatom systematics and palaeoecology have been studied. A marine diatom assemblage was found in the lower part of the sedimentary sequence and a rich freshwater assemblage, including more than 200 taxa, was found in the upper part. Some of the characteristic diatoms are typical of standing or slowly-moving waters of more southern and western districts but are not found in the Bolshezemelskaja tundra lakes today. According to 14C dating, the age of the deposit is about 40 000 years, and represents the Middle-Valdaj (Middle-Weichselian). Palynological and diatom analysis suggest that the climate was similar to the present, that of an interglacial.
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    Hydrobiologia 103 (1983), S. 281-285 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Lake Baikal ; Pliocene ; Pleistocene ; pollen ; diatoms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The following stages in the formation of sediments in Lake Baikal have been distinguished. In the Middle Miocene lacustrine deposits accumulated in the southern depression. In Early and Middle Pliocene, as well as in Early-Middle Pleistocene the bottom of the southern and middle Baikal depressions was occupied by large lakes, but the northern basin was mainly dry with a system of small lakes, streams and rivers. This accounts for the great facies variety and the genetic heterogeneity of sediments in the northern depression. At the end of Middle and in the Late Pleistocene an abrupt warping of the depression took place, and a shore-line similar to the present one was formed. Sands in the upper part of the sediments are considered to be the product of intensive erosion which probably occurred in the pluvial epoch in the Late Pleistocene period.
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    Hydrobiologia 103 (1983), S. 287-293 
    ISSN: 1573-5117
    Keywords: paleolimnology ; sedimentation ; sediment laminations ; diatom microstratigraphy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Laminated sediment is deposited in the deep areas (〉 30 m) of Lake Pääjärvi. In the diatom microstratigraphy a regular alteration of planktonic and littoral floras, which goes in pace with the visual structure, reflects the yearly cycle of sedimentation. Considerable variation in sedimentation was found to have occurred during the past two decades. A sequence, dated to the early 1960's, in which the organic content was exceptionally high, could be identified in the sediment at 6–14 cm depth even in shallower areas where laminations are not formed. This level was used as a marker horizon when determining the recent distribution of sediment by means of 19 cores taken from different parts of the lake. The average annual sediment accumulation in the profundal zone of the lake is 340 g m−2a−1 dry weight, of which 25 g m−2 a−1 (1100 kJ m−2 a−1) is organic carbon.
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    Hydrobiologia 103 (1983), S. 295-301 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Flandrian biostratigraphy ; Tabellaria binalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Early and Middle Flandrian geological development and paleolimnology of Lake Spitaalijärvi was studied using pollen, diatom and cladoceran analysis and 14C dating. Spitaalijärvi was isolated from the Ancylus Lake about 9000 B.P., at which time birch and pine and plants typical of open habitat communities grew on the solitary island. The rational limits for Alnus and Picea were ca. 8300 B.P. and 3700 B.P., respectively. During the first few hundred years after isolation Spitaalijärvi was probably fairly eutrophic, with a low water level. Water level began to rise before the Alnus rise (A°) and the lake became oligotrophic. After another transgression, which started before the Picea rise (P°), Tabellaria binalis and Semiorbis hemicyclus appear in the diatom stratigraphy indicating ultra-oligotrophic conditions. The main reason for the ultra-oligotrophy of Lake Spitaalijärvi is the character of the parent material in the catchment, consisting of glacial and littoral deposits derived from the local quartz sandstone of highly siliceous nature.
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    Hydrobiologia 103 (1983), S. 303-308 
    ISSN: 1573-5117
    Keywords: paleolimnology ; acid waste-water ; liming ; diatoms ; heavy metals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lake Sysmäjärvi is located inside the municipal boundary of Outokumpu in North Karelia, Finland. Since 1928 metalliferous, and at a later stage highly acid waste-water, has been discharged into the lake. Neutralization of the lake water by liming was introduced, and by the end of 1966 the pH of the water had become neutral to alkaline. Two sediment cores (0.5 m and 5 m long, respectively) were analyzed for pollen, diatoms, loss-on-ignition, organic carbon, Fe, Cu, Ni, Pb, Zn and Hg. The maximum concentrations of organic matter and heavy metal content occur 30 cm below the sediment surface. Evidence of the liming and of accelerated eutrophication appear close to the surface of the sediment (0–10 cm), indicated by an increase in Nitzschia plana.
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    Hydrobiologia 103 (1983), S. 309-318 
    ISSN: 1573-5117
    Keywords: paleolimnology ; heavy metals ; polluted lake ; sedimentary chemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Macro nutrients N, P, K, Ca, Mg, Na and S as well as heavy metals Pb, Cu, Zn, Co, Cr, Ni, Fe and Mn were analysed from a sediment core representing a 7000-year long history of a lake including the preceding brackish (Litorina Sea) and freshwater (Ancylus Lake) stages. Sediments were also analysed for sedimentary pigments, algal growth potential (AGP) of the interstitial water, phosphorus fractions, pH, specific conductivity and redox potential. The annual accumulation values of several chemical elements were interpreted in light of other paleolimnological data from the same core. In the uppermost sediment stratum the influence of sewage pollution is clearly reflected by increases in total electrolyte content, pH, AGP and sedimentary pigments including myxoxanthophyll. Nickel, copper and manganese only slightly increase in the recent sediment, whilst other heavy metals (especially Cr and Zn) and sulphur, phosphorus, nitrogen and total organic matter are heavily concentrasted in the polluted surface sediment. The interpretation of the chemostratigraphy of the lake accords with evidence of lake development based on biological analyses.
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    Hydrobiologia 103 (1983), S. 15-19 
    ISSN: 1573-5117
    Keywords: paleolimnology ; dating ; radioactive fallout ; sediment ; 137Cs ; 239,240Pu ; varves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of 137Cs and 239,240Pu in sediment core samples of the Finnish lakes Laukunlampi, Lovojärvi and Pääjärvi were determined. The sediment samples were collected using dry ice and liquid nitrogen freezing methods. The sediments of these lakes are annually laminated. A clear maximum concentration of 137Cs and 239,240Pu was found in sediment layers formed during 1962–1964, the years of maximum fallout, and the middle of the 1950's can be estimated from the 137Cs and 239,240Pu profiles. The highest concentrations, 11 500 and 820 pCi kg−1 dry wt for 137Cs and 239,240Pu, respectively, were found in the sediment of Laukunlampi. The vertical distribution was similar for 137Cs and 239,240Pu in the lakes investigated. A slight migration of 239,240Pu and 137Cs was found and the migration of 137Cs seems to be higher than that of 239,240Pu. The advantages of 137Cs dating method are rapidity and simplicity. 239,240Pu is preferable when the sample size is small. The agreement found between 137Cs and 239,240Pu dates and the annual laminae show that these fallout radio isotopes can be used for dating sediments formed during the past 25 years.
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    Hydrobiologia 103 (1983), S. 29-35 
    ISSN: 1573-5117
    Keywords: paleolimnology ; 210Pb ; sediment accumulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The last few years have seen a dramatic growth in the use of 210Pb sediment dating. Despite this, considerable doubt still surrounds the nature of the processes by which 210Pb is deposited in lake sediments, and this has lead to a situation where there is a choice of dating models offering different interpretations of 210Pb data. In assessing 210Pb data it is therefore essential to first of all determine whether data is consistent with the assumptions of the dating model, and to then compare the 210Pb chronology with independent dating evidence. We have tested 210Pb data from a wide variety of sites, and our calculations indicate that the crs (constant rate of 210Pb supply) model provides a reasonably accurate chronology when the total 210Pb contents of cores from neighbouring locations are comparable.
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  • 97
    ISSN: 1573-5117
    Keywords: paleolimnology ; caesium-137 ; perched lake ; meromictic ; diffusion ; bioconcentration ; sediment accumulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hidden Lake is a perched, brown-water lake located in the centre of Great Sandy Island (Fraser Is.), S.E. Queensland. It is highly acid (pH 4.0), oligotrophic and is thermally and chemically stratified for most of the year. The sediments revealed a 137Cs profile which departed from the temporal pattern of 137Cs fallout in Brisbane and was represented by an exponential increase of 137Cs towards the surface sediments from ca. 32 cm depth. The possible causes of the divergent profile are discussed, including physical and biological mixing, lag in the transport of catchment material to the sedimentary basin, diffusion, recycling and biological concentration. It is hypothesised that a combination of the last four processes, with diffusion facilitated by the highly acid conditions, are the major causes of the observed 137Cs profile. Possible recycling and bioconcentration of 137Cs raises questions as to the validity of this method of dating in similar environmental conditions, and as to the interpretation of other palaeochemical data. These hypotheses are to be tested against profiles obtained from 14C, 210Pb, 239/240Pu analyses of the sediment, and the measurement of 137Cs activity in the water and biota of the lake.
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  • 98
    ISSN: 1573-5117
    Keywords: paleolimnology ; pollen analysis ; land drainage ; sediment accumulation ; 210Pb dating ; 14C reservoir effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previous measurements of the 14C activity of lake sediments and Potamogeton plants from Säynäjälampi, collected in 1970, indicated a severe hard-water effect (Donner et al. 1971). Samples from new cores, collected in 1977, were sliced into thin sections down to 18 cm. The 14C concentration in samples down to 7 cm was similar to that obtained by the Helsinki Laboratory. The corresponding apparent 14C age is almost 2 000 years. Light-coloured layers, apparently due to catchment drainage, were used together with density diagrams to correlate the cores. The drainage history gives a time-scale showing that the lowest samples dated to correlate the cores. The drainage history gives a time-scale showing that the lowest samples dated were just old enough to be pre-bomb sediments. Three samples of submerged plants were also examined, indicating some exchange with the air and an appreciable reservoir age. The activity of the plants was significantly lower in 1978 than in 1970. One sample, well below the lowest drainage stratum, indicates a reservoir age of ca. 1 100 years. This sample yielded an apparent age of the INS fraction which was significantly different from that of the SOL fraction. The difference was determined to ca. 600 years. Total 210Pb was determined via 210Po using isotope dilution while 226Ra was measured with the help of radon emanation. The 226Ra was used to estimate the supported 210Pb. The unsupported 210Pb showed a higher sediment accumulation rate for the top nine cm of one core (the length investigated was 16.5 cm). The varying accumulation rate was explained by human activities, as could be seen from the heterogenous sediment composition of the cores. Recent vegetational change is described from direct observations since 1957. Pollen analysis does not give any reliable indication of agriculture.
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    Cell & tissue research 228 (1983), S. 205-218 
    ISSN: 1432-0878
    Keywords: Spermatozoa ; Ultrastructure ; Deep-freezing ; Sperm manipulation ; Trout
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Trout spermatozoa taken from the testis, vas deferens and ejaculate are described at the ultrastructural level. The morphology of the spermatozoa head, changes in the middle-piece structure, and the relationship between the centrioles and the flagellum were studied under consideration of their role in the reproduction of this species. Morphological changes observed after dilution of the spermatozoa in freshwater or saline and subsequent freezing deserve attention in connection with certain manipulations used in fish farms and laboratories.
    Type of Medium: Electronic Resource
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  • 100
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 228 (1983), S. 231-244 
    ISSN: 1432-0878
    Keywords: Pineal gland ; Circadian rhythm ; Synaptic ribbon ; Ultrastructure ; Chinese hamster (Cricetulus griseus)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Circadian morphological variations of pinealocytes in the superficial pineal of the Chinese hamster (Cricetulus griseus) were studied using quantitative electron-microscopic techniques. The volume of the nucleus and cytoplasm of pinealocytes exhibited similar circadian variations, with the maximum around the middle of the light period and the minimum during the first half of the dark period. Synaptic ribbons in pinealocytes were classified into three groups, type-1, −2 and −3 synaptic ribbons, which appeared as rods, round or irregular bodies and ring-shaped structures, respectively; a synaptic ribbon index was determined for the respective types. The synaptic ribbon index was expressed as the number of synaptic ribbons in the pinealocyte profile representing the cell size. The type-1 synaptic ribbon index, which was smallest during the second half of the light period, was increased during the dark period. The length of straight or slightly curved rods showed a 24-h change similar to that of the type-1 synaptic ribbon index; the length of the rods was maximal during the first half of the dark period and minimal at the end of the light period. There was no apparent circadian variation in the type-2 synaptic ribbon index. The type-3 synaptic ribbon index was higher during the light period than during the dark period; the index attained zero 3h after the onset of darkness and, thereafter, increased gradually.
    Type of Medium: Electronic Resource
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