ISSN:
1615-6102
Keywords:
Endoplasmic reticulum
;
Golgi apparatus
;
Myxamoebae
;
Physarum polycephalum
;
Zinc impregnation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The post-fixation ofPhysarum polycephalum myxamoebae with a zinc iodide-osmium tetroxide mixture resulted in the accumulation of an electron dense deposit in the lumen of the nuclear envelope, the endoplasmic reticulum, and most of the cisternae of the Golgi apparatus. In double-stained thin sections of impregnated myxamoebae the preservation of other cytoplasmic organelles was excellent. Examination of thick sections (0.25 μm) at 120 kV revealed the complexity of the endomembrane system and continuities between both the ER and the nuclear envelope, and the ER and the Golgi apparatus. No direct continuities were observed between the cisternae of the Golgi apparatus and the nuclear envelope. A three dimensional view of membrane organization was obtained from stereopairs, while tilting the sections at much steeper angles revealed whether any of the apparent continuities seen were real or were simply the result of overlap. A morphologically distinct region of the ER, which bears similarities to the GERL region described in other organisms, was found in continuity with the remaining ER, the Golgi apparatus, and the nuclear envelope.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01279722
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