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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European journal of nutrition 23 (1984), S. 12-19 
    ISSN: 1436-6215
    Keywords: Energie ; Wärmeproduktion ; Realimentation ; Nüchternumsatz ; Erhaltungsumsatz
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary A metabolism trial with six adult sows was conducted to study the effect of fasting and realimentation on heat production, energy retention, and utilization of energy. Complete balance measurements by indirect calorimetry (CN-method, RQ-method) were carried out from the 8th to the 10th day of fasting, and thereafter from the 1st to the 7th day and from the 33rd to the 36th day of the realimentation phase. Previous to fasting and during the time of refeeding the energy supply was adapted to maintenance requirement. Heat production rose by 20% after refeeding the animals. This heat increment corresponded to 2.2 MJ/kg feed dry matter, or 12% of the energy intake, respectively. The stimulation of heat production induced by refeeding occurred spontaneously within one day. After the first day of refeeding a plateau was reached, which in the course of the steady energy supply was still present even after the 5th week of realimentation. Residual effects of fasting in the mode of a compensatory heat production were not observed. Therefore, a constant utilization of efficiency of metabolizable energy for maintenance was estimanted at 82%. The change of body energy during the entire experimental period resulted in a loss of 198 MJ corresponding to about 5% of the body weight.
    Notes: Zusammenfassung In einem Gesamtstoffwechselversuch mit 6 ausgewachsenen Sauen wurden Wärmeproduktion und Energieretention bei Nüchterung und nachfolgender kontrollierter Realimentation untersucht. Die quantitative Stoffwechselmessung mit Hilfe der indirekten Kalorimetrie (CN-Methode und RQ-Methode) fand am 8.–10. Hungertag und am 1.–7. sowie 33.–36. Tag der Wiederfütterung statt. Die Nahrungszufuhr war vor und nach der Hungerphase auf den energetischen Erhaltungsbedarf ausgerichtet. Die Wärmeproduktion lag in der Realimentation gegenüber der Hungerphase um 20% höher. Dieser Wärmezuwachs entsprach 2,2 MJ/kg Futtertrockenmasse bzw. 12% der Energieaufnahme. Der Energiewechsel adaptierte sich innerhalb eines Tages an die Situation der Wiederfütterung. Danach blieb die Wärmebildung bzw. die Energieretention bis Ende des Versuchs auf einem konstanten Niveau. Nachwirkungen der Nüchterung im Sinne einer kompensatorisch veränderten Wärmeproduktion traten nicht auf. Die energetische Verwertung der umsetzbaren Energie für Erhaltung berechnete sich auf 82%. Die über die gesamte Versuchszeit erstellte Energiebilanz ergab ein Defizit der Körperenergie von 198 MJ, entsprechend etwa 5% des Körpergewichtes.
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  • 2
    Electronic Resource
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    Springer
    European journal of nutrition 23 (1984), S. 31-40 
    ISSN: 1436-6215
    Keywords: endogener Kotstickstoff ; bakterieller Stickstoff ; Guarmehl ; 15N-Tracer-Technik ; Ratten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The metabolic fecal nitrogen was determined in 8 young rats (90–100 g body weight) using a single oral dose of 75 mg15N-glycine. Four rats were fed a diet containing 8 % guar and 4 rats received a control diet without guar. Compared to the control group, apparent protein digestibility and nitrogen balance were significantly lower in the guar group. After an initial steep rise the15N-elimination in feces and urine (days 2–8 after15N-glycine application) followed an exponential curve in at least 2 phases, with increasing half-time values. The proportion of metabolic fecal nitrogen to total fecal nitrogen was calculated from the15N-atom-% excess, determined daily in urine and in feces. This calculation is based on the assumption that the specific15N-activity (15N-atom-% excess) in urine represents the specific15N-activity of the endogenous nitrogen pool. The addition of guar raised the proportion of metabolic fecal nitrogen from 51 % (control) to 73 %, corresponding to an increase in total fecal nitrogen loss (24 mg) observed in the guar-fed animals. This indicates that the rise in fecal nitrogen loss caused by guar is due almost entirely to enhanced secretion of endogenous nitrogen and not to undigested food protein. Based on the analysis of 2,6-diamino-pimelic acid in the feces of 4 rats the proportion of bacterial nitrogen in the feces was calculated. It amounted to 19.8 % of total fecal N in the control group, and to 30.1 % in the guar group, or to 39.4 % and 44.4 % of metabolic fecal nitrogen respectively. Obviously the increased dietary fiber intake also caused a rise in the amount of metabolic fecal nitrogen, which is derived from gastro-intestinal secretions and epithelial tissue.
    Notes: Zusammenfassung Zur Bestimmung der endogenen Stickstoffausscheidung im Kot wurde acht jungen Ratten (90 bis 100 g Körpergewicht) eine orale Dosis von 75 mg15N-Glycin verabreicht. Vier Ratten erhielten eine 8%ige Guarmehldiät und vier Ratten eine Kontrolldiät ohne Guarmehlzusatz. Gegenüber der Kontrollgruppe wurde eine signifikante Verringerung der scheinbaren Proteinverdaulichkeit und der N-Bilanz in der Guarmehlgruppe festgestellt. Nach einem anfänglichen steilen Anstieg verlief die15N-Elimination in Kot und Urin vom 2. bis 8. Tag nach der15N-Glycin-Applikation exponentiell in mindestens zwei Phasen mit steigender Halbwertszeit. Aus den experimentell ermittelten Tageswerten des15N-Atom-%-Überschusses in Urin und Kot wurde der Anteil des endogenen Stickstoffs im gesamten Kotstickstoff berechnet. Hierbei wurde vorausgesetzt, daß die spezifische15N-Aktivität des Urins (15N-Atom-%-Überschuß) dem15N-Markierungsgrad des endogenen Stickstoff-Pools entspricht. Durch Guarmehlzusatz erhöhte sich der Anteil des endogenen Kotstickstoffs von 51% (Kontrollgruppe) auf 73%, was einem Anstieg von 10 mg N/Tag auf 32 mg N/Tag entspricht. Diese Differenz von 22 mg N/Tag ist vergleichbar mit dem um 24 mg/Tag erhöhten N-Verlust im Kot der Tiere in der Guarmehlgruppe. Demnach sind die durch Guarmehl verursachten erhöhten N-Verluste fast ausschließlich durch eine vermehrte endogene N-Sekretion und nicht durch unverdautes Nahrungsprotein bedingt. Mit Hilfe der 2,6-Diamino-pimelinsäure-Analyse des Kots von vier Ratten wurde der Anteil des aus Darmbakterien stammenden Kot-N bestimmt. Er betrug 19,8 % (Kontrollgruppe) bzw. 30,1% (Guarmehlgruppe) des Gesamt-Kot-N bzw. 39,4% (Kontrollgruppe) und 44,4 % (Guarmehlgruppe) des endogenen Kot-N. Hieraus ist zu folgern, daß die erhöhte Ballaststoffzufuhr auch eine vermehrte Ausscheidung an endogenem Stickstoff in Form von N-haltigen Sekreten und Epithelzellen des Magen-Darm-Traktes bewirkt hat.
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  • 3
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    Springer
    European journal of nutrition 23 (1984), S. 58-65 
    ISSN: 1436-6215
    Keywords: Feststoffsubstrate ; Kolonienbildung von Mikroorganismen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Bacteria have been found to grow differently at contact sites to both organic and anorganic solids as on pure agar media. Of 16 ad hoc selected bacterial strains, the following species (arranged in decreasing order) responded with stronger colony formation: Bacillus subtilis, B. cereus, Streptococcus sanguis, S. saprophyticus, S. faecalis, Escherichia coli, Micrococcus flavus, Actinomyces viscosus, Veillonella sp., Enterobacter liquefaciens, Yersinia enterocolitica. Lactobacillus casei behaved neutral. Proteus vulgaris, Y. enterocolitica and Chromobacterium violaceum grow on certain media only in the presence of solid particles. The yeasts Endomyces lactis, Saccharomyces cerevisiae, Rhodotorula mucilaginosa and Cryptococcus nigricans did not respond to solid particles during colony formation. The kind of protein — animal (meat) or vegetable (soy beans) — did not influence the behaviour of the microorganisms in these experiments.
    Notes: Zusammenfassung Es wurde festgestellt, daß die Bakterien an Kontaktstellen mit sowohl organischen als auch anorganischen Feststoffmaterialien anders wachsen als auf reinen Agarnährböden. Von 16 ad hoc gewählten Bakterienstämmen reagierten folgende Spezies mit intensiverer Kolonienbildung in sinkender Reihenfolge: Bacillus subtilis, B. cereus, Streptococcus sanguis, S. saprophyticus, S. faecalis, Escherichia coli, Micrococcus flavus, Actinomyces viscosus, Veillonella sp., Enterobacter liquefaciens, Yersinia enterocolitica. Lactobacillus casei verhielt sich neutral. Proteus vulgaris, Y. enterocolitica und Chromobacterium violaceum wachsen auf bestimmten Nährböden nur in Anwesenheit der Festpartikel. Die Hefen Endomyces lactis, Saccharomyces cerevisiae, Rhodotorula mucilaginosa und Cryptococcus nigricans zeigten bei der Kolonienbildung keine Reaktion auf die Feststoffpartikel. Die Art des Proteins in Nährböden — tierisch (Fleisch) oder pflanzlich (Sojabohnen) — beeinflußte das Verhalten der Mikroorganismen in diesen Untersuchungen nicht.
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  • 4
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    Springer
    European journal of nutrition 23 (1984), S. 104-112 
    ISSN: 1436-6215
    Keywords: vitamin A ; inner ear ; review ; vitamin A deficiency ; hearing loss
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Since the first characterization and description of vitamin A this is used in otolaryngologic therapy for different forms of hearing disorders, and its relation to the inner ear is subject of investigation. Animal experiments and clinical studies were done to clarify the significance of vitamin A for the function of hearing. Besides this there were a lot of observations describing correlations between vitamin A metabolism and hearing loss. Recent investigations showed that vitamin A is present in high concentrations in the inner ear and stored there. Morphological experiments revealed different and in some way contradictory results, but they showed that vitamin A seems to be essential for inner-ear morphogenesis.
    Notes: Zusammenfassung Seit der Entdeckung des Vitamin A und ersten Beschreibung seiner Wirkung wird dieses auch in der Otorhinolaryngologie als Therapeutikum bei den unterschiedlichsten Formen von Hörstörungen eingesetzt und seine Wirkungsweise am Innenohr erforscht. So wurden sowohl tierexperimentelle als auch klinische Studien durchgeführt, um die Bedeutung des Vitamins für die Funktion des Hörorgans zu klären. Daneben wurden eine Reihe von Beobachtungen mitgeteilt, die einen Zusammenhang zwischen Störungen des Vitamin-A-Stoffwechsels und gleichzeitig auftretenden Hörstörungen beschrieben. Neuere Untersuchungen haben ergeben, daß Vitamin A in hohen Konzentrationen im Innenohr vorkommt und dort auch gespeichert werden kann. Morphologische Untersuchungen führten zu den unterschiedlichsten Ergebnissen und haben gezeigt, daß Vitamin A für die Morphogenese des Innenohres von Bedeutung zu sein scheint.
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  • 5
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    Springer
    European journal of nutrition 23 (1984), S. 276-282 
    ISSN: 1436-6215
    Keywords: starvation ; brain ; lipid metabolism ; dietary lipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Der Einfluß verschiedener Nahrungsfette auf den Lipidstoffwechsel des Gehirns während Hunger wurde untersucht. Albinoratten wurden 8 Tage lang mit Dalda, Senföl oder Erdnußöl gefüttert. Einige Tiere blieben anschließend 3 und 5 Tage lang ohne Nahrung. Die Gesamtlipide und die Galaktolipide wurden durch Nahrungsfette und durch Hunger nicht beeinflußt, während die Phospholipide unabhängig von der Art des Nahrungsfetts signifikant zunahmen. Cholesterin nahm in den mit Dalda und mit Erdnußöl gefütterten Gruppen zu und nahm ab während des Hungerns. In der Hungerperiode im Anschluß an die Fettzufuhr war der Einbau von 1-14C-Acetat in Lipide von Gehirnschnitten nach Erdnußöl höher als nach Dalda. Nach Senföl war der Einbau nur gering.
    Notes: Summary In this study the effects of the dietary fat sources, viz., Dalda, mustard oil and groundnut oil in the brain lipid metabolism during starvation has been investigated. To find out this, these oils were fed to albino rats for 8 weeks followed by 3 and 5 days of starvation. Total as well as galactolipids of brain were not affected by dietary oils and starvation, whereas phospholipids of brain were significantly increased by fasting irrespective of the dietary fats. On the other hand, cholesterol was found to be increased in the groups fed with Dalda and groundnut oil and to be significantly decreased during fasting experiments. Incorporation of (1-14C)acetate into lipid of the brain slices of rats fed different experimental diets than followed by starvation revealed that the uptake of radioactivity was higher in the group fed with groundnut oil, followed by Dalda. Low uptake was observed in the group fed with mustard oil.
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  • 6
    ISSN: 1436-6215
    Keywords: germ-free rat ; intestinal tract colonization ; interaction ; protection ; Bifidobacterium ; E. coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Das Vorherrschen von Bifidobakterien in normalen, mit Muttermilch ernährten Säuglingen ist gut dokumentiert. Sogar unter ungünstigen hygienischen Bedingungen bei der Geburt und während der Stillperiode entwickeln sich die Bifidobakterien und kolonisieren den Darmtrakt in großer Anzahl. In dieser Studie untersuchten wir die Wechselwirkung zwischen Bifidusbacterium longum und einem pathogenen E.-coli-Stamm in der keimfreien Ratte. Die Proliferation der beiden Bakterienstämme wurde durch sequenzielle Probeentnahme verfolgt. Die licht-mikroskopische und elektronen-mikroskopische Untersuchung von ausgewählten Darmabschnitten zeigte geringgradige morphologische Veränderungen. Die Bifidobakterien schützen die Ratten vollständig gegen Mortalität durch nachfolgende Infektion durch E. coli. Wiederholte Verabreichung von Bifidobakterien verminderte darüber hinaus die E.-coli-Population und hielt diese auf einem niedrigen Niveau. Daher erscheint die keimfreie Ratte als ein angemessenes Modell zum Studium der Entwicklung und Wechselwirkung von beiden Bakterienstämmen. Die Sequenz der Inokulation ist dabei von großer Bedeutung.
    Notes: Summary The predominance of Bifidobacteria in normal breast-fed babies is well established. Even under unfavourable hygienic conditions of delivery and during the breast-feeding period Bifidobacteria develop and colonize the intestinal tract at high concentrations. In the present study we investigated the interaction between Bifidobacterium longum and a pathogenic E. coli strain in the germ-free rat. Sequential counts of the two bacterial strains allowed their proliferation to be followed. Electron microscopic as well as light microscopic examinations of selected intestinal mucosa segments revealed minor morphological changes. Bifidobacterium completely protected the rats against mortality from a consecutive infection with E. coli. Repeated inoculation of Bifidobacteria even decreased and kept down the initial E. coli population. Thus it appears that the germ-free rat is an appropriate model to study the development and interaction of both bacterial species and that the sequence of inoculation is most important.
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  • 7
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    European journal of nutrition 23 (1984), S. 74-74 
    ISSN: 1436-6215
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
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  • 8
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    European journal of nutrition 23 (1984), S. 75-75 
    ISSN: 1436-6215
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
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  • 9
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    European journal of nutrition 23 (1984), S. 143-150 
    ISSN: 1436-6215
    Keywords: rice starch ; cooking quality ; physicochemical properties ; storage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung 12 verschiedene Sorten von Reis aus Indien wurden im Hinblick auf die Auswirkung einer Lagerung bei verschiedenen Umgebungsbedingungen für die Dauer von 180 Tagen auf physikalisch-chemische Eigenschaften des Stärkeanteils und auf die notwendigen Garzeiten sowie die beim Garen eintretenden Verluste an Trokkensubstanz bzw. die Wasseraufnahme untersucht. Die Viskosität der Stärke nahm bei allen Reissorten während der Lagerung zu. Bezüglich der Garungszeiten waren keine größeren Unterschiede zwischen den Sorten zu beobachten, während die Wasseraufnahmen sehr unterschiedlich sein konnten.
    Notes: Summary Twelve different varieties of rice including two flavorful varieties grown in the State of Maharashtra (India) have been studied with respect to the effect of storage, at ambient conditions, for 180 days on the physicochemical properties of the starch component as well as cooking quality.
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  • 10
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    European journal of nutrition 23 (1984), S. 157-170 
    ISSN: 1436-6215
    Keywords: Dünndarmresorption ; Tumorstadium ; histologischer Typ ; Organlokalisation ; Ernährungstherapie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary A deteriorating nutritional condition combined with the loss of body mass is one of the most noticeable symptoms of cancer. In order to answer the question to what extent absorption disorders in the small intestine are responsible for the malnutrition which develops, we studied 54 oncologic patients using modified D-xylose absorption tests. After the oral and intravenous administration of 10 g D-xylose at different times, the degree of absorption dependent on time was determined on the basis of a biophysical model. When accompanied by general nonspecific tumor symptoms, the frequency of absorption restrictions was increased. An established relation was found between the extent of small intestinal absorption, on the one hand, and the degree of spreading (staging) and the degree of histological differentiation of the carcinoma, on the other hand. Enteral absorption disorders represent a partial cause for the development of malnutrition in cancer patients and must be viewed as a result of a metabolic situation which is distinctly catabolic.
    Notes: Zusammenfassung Die Verschlechterung des Ernährungszustandes und die Abnahme an Körpermasse ist eines der auffälligsten Symptome des Krebsleidens. Zur Beantwortung der Frage, inwieweit Störungen der Dünndarmresorption für die sich entwickelnde Malnutrition verantwortlich zu machen sind, untersuchten wir 54 onkologische Patienten mit Hilfe des modifizierten D-Xylose-Resorptionstests. Nach zeitlich getrennter oraler und intravenöser Applikation von 10 g D-Xylose wurde auf der Grundlage einer biophysikalischen Modellierung das Ausmaß der Resorption in Abhängigkeit von der Zeit ermittelt. Mit dem Auftreten allgemeiner unspezifischer Tumorsymptome erhöht sich die Häufigkeit von Resorptionseinschränkungen. Es konnte eine gesicherte Beziehung zwischen Ausbreitungsgrad (Staging), histologischem Differenzierungsgrad des Karzinoms einerseits und dem Ausmaß der Dünndarmresorption andererseits nachgewiesen werden. Enterale Resorptionsstörungen stellen eine Teilursache bei der Entwicklung einer Mangelernährung bei Krebskranken dar und sind selbst als Folge der katabol akzentuierten Stoffwechsellage anzusehen.
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  • 11
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    European journal of nutrition 23 (1984), S. 219-229 
    ISSN: 1436-6215
    Keywords: Maillard-Produkte ; Protein-Qualität ; Wachstum und Nephrozytomegalie bei Ratten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Two experiments lasting 10 vs. 8 weeks respectively were conducted in order to determine the effects of feeding heated proteins (casein + glucose, 65 °C for 4 days) on histopathological alterations in the kidneys of rats. Histological examinations showed enlarged epithelial cells and cell nuclei in the pars recta of the proximal tubules (inner stripe of the inner kidney cortex zone) beginning after two weeks of the test. The differences in the average size of the nuclei were significant after a feeding period of 6, 8 or 10 weeks (44 rats in the testgroup vs. 36 rats in the controlgroup respectively). In this way the alterations are very similar to that which are induced by lysinoalanine. The substance causing the lesions seems not to be fructoselysine although it was present in the heat damaged diets in concentrations of 16,000–23,000 ppm but is suggested among the substances formed in the early and advanced stage of Maillard reaction.
    Notes: Zusammenfassung In zwei Versuchen von 10 bzw. 8 Wochen Dauer wurde der Einfluß der Fütterung eines hitzegeschädigten, lysinoalaninfreien Proteins (Casein+Glucose, 65 °C, 4 Tage) auf histopathologische Veränderungen in Rattennieren untersucht. In den histopathologischen Präparaten der Nieren waren schon nach zwei Wochen vergrößerte Tubulusepithelzellen in der pars recta des proximalen Tubulus zu erkennen. Zellkernmessungen in der 6., 8. und 10. Versuchswoche ergaben eine signifikante Erhöhung der mittleren Zellkerngröße bei den Versuchstieren (n=44) im Vergleich zu den Kontrolltieren (n=36). Damit waren die Veränderungen vergleichbar mit den aus der Literatur bekannten, durch Lysinoalanin induzierten histopathologischen Befunden. Das auslösende Agens für die Nephrocytomegalie konnte nicht identifiziert werden. Es dürfte im Bereich der vielen Zwischenprodukte aus dem Initial- und Zwischenstadium der Maillard-Reaktion zu suchen sein.
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  • 12
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    European journal of nutrition 23 (1984), S. 113-125 
    ISSN: 1436-6215
    Keywords: Muttermilch ; Triglyceride ; Cholesterin ; Phospholipide ; Fettsäuren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The dependency of the human milk lipids triglycerides (TG), cholesterol (Chol), phospholipids (PL), and the fatty acid composition of both TG and PL on the stage of lactation, the day time, the course of one nursing, and on the mother's diet was shown by analyzing more than 350 milk samples. In progressing lactation (day 1–36) TG increased from 1.9 to 3.9 g/100 ml. Chol decreased from 35 to 20 mg/100 ml, while PL remained with 40 mg/100 ml rather constant. The resulting decrease in the PL/TG- and Chol/TG-ratios indicates an increasing size of fat globules. During one nursing, TG and Chol increased four-fold and PL doubled. During the day lipids (TG, Chol, PL) of the milks of German mothers reached their peak values in the afternoon, whereas the ones of milks obtained from English mothers were found in the late evening. With 3 mothers (n=3) carbohydrate-rich diets (65 cal%) led to an increase of the milk fat content. In contrast high fat diets (50 cal%) resulted in milks with low fat contents (2.6/4.1 g/100 ml). Chol (20.8/37.5 mg/100 ml) and PL (26.4/ 45.3 mg/100 ml) showed the same dependency. The fatty acid composition changed mainly dependent on the duration of the lactation and the mother's diet, but not during one single nursing and during the day. Long-chain polyunsaturated fatty acids were higher in early than in mature milks. The P/S-ratio of TG was found to remain constant in progressing lactation (0.32), whereas the one of PL increased from 0.6 to 0.71. Dependent on the regimen of the mother the P/S-ratios (n=3) were found to be 0.27 (high carbohydrate diet) and 0.46 (high fat/high linoleic acid diet).
    Notes: Zusammenfassung Mit der Analyse von über 350 Frauenmilchproben konnte die Abhängigkeit der Frauenmilchlipide — Triglyceride (TG), Cholesterin (Chol), Phospholipide (PL), Fettsäurezusammensetzung der TG und PL — vom Stadium der Laktation, der Tageszeit, dem Verlauf des Stillvorgangs und der mütterlichen Ernährung gezeigt werden. Im Verlauf der Laktation (1.–36. Tag) nahm der TG-Gehalt der untersuchten Proben von 1,9 auf 3,9 g/100 ml zu. Chol sank von 35 auf 20 mg/100 ml. Der PL-Gehalt blieb mit 40 mg/100 ml weitgehend konstant. Die sich aus den Ergebnissen ableitenden Chol/TG- und PL/TG-Quotienten deuten auf eine Zunahme der Fettkügelchengröße im Laufe der Laktation hin. Während eines Stillvorganges stiegen die TG und Chol um das nahezu Vierfache an, wogegen sich der PL-Gehalt nur verdoppelte. Über den Tag gesehen hatten Milchen deutscher Mütter ihre höchsten Lipidwerte (TG, Chol, PL) am Nachmittag, wogegen die Maxima der englischen Milchproben am späten Abend gefunden wurden. Kohlenhydratreiche Ernährungsweise (65 cal%) führte bei Müttern (n=3) zu einem Anstieg des Milchfettgehalts, wogegen umgekehrt aus fettreicher Ernährung relativ fettarme Milchen resultierten (2,6/4,1 g/100 ml). Entsprechend verhielten sich Chol (20,8/37,5 mg/ 100 ml) und die PL (26,4/45,3 mg/100 ml). Die Fettsäurezusammensetzung änderte sich vor allem in Abhängigkeit von der Laktation und der mütterlichen Ernährung, nicht aber während des Stillvorganges und im tageszeitlichen Verlauf. Frühe Milch ist besonders reich an langkettig polyungesättigten Fettsäuren. Der P/S-Quotient der TG blieb im Verlauf der Laktation mit 0,32 weitgehend konstant, wogegen derjenige der PL von 0,6 auf 0,71 anstieg. Je nach Ernährungsweise der Mutter lag der P/S-Quotient (n=3) bei 0,27 (kohlenhydratreich) und bei 0,46 (fettreich, linolsäurereich).
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  • 13
    ISSN: 1436-6215
    Keywords: tierexperimenteller Thiaminmangel ; Pyruvatverwertungsstörung ; Laktazidose ; Aminosäurehomöostasestörung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The metabolic effects of thiamine deficiency on the metabolism of lactate, acid-base and amino acids were evaluated both with and without muscular exercise in a controlled animal experiment. Thiamine deficiency — in good correlation with its biologic halftime and also with reports in the literature — caused with a latency of 17–56 days and statistically proven in parallel the following effects: 1. hyperlactemia 2. in comparison to controls elevated plasma concentrations of threonine, alanine, glutamic acid, glutamine, histidine, methionine, taurine, valine, isoleucine and leucine 3. distinctly lowered — also in comparison to controls — plasma concentrations of arginine and tryptophan. Furthermore, in the thiamine deficient rats predetermined muscular exercise caused the following effects, also occurring concomitantly: 1. life threatening lactacidosis 2. marked — statistically prover — disturbance of the homeostasis of alanine, glycine, methionine and arginine 3. distinctly — and statistically significant — elevated plasma concentration of proline 4. statistically significantly lowered plasma concentrations of taurine. The reasons for all these changes under thiamine deficiency is most likely a metabolic one primarily due to a disturbance of the utilization of pyruvate in the liver. An additional disturbance of both the transsulfuration pathway and the Krebs-Henseleit-cycle seems probable. The physiologic regulation of pyruvate, lactate, alanine, serine and tyrosine during muscular exercise in correlation to the controls is being discussed.
    Notes: Zusammenfassung In einer kontrollierten tierexperimentellen Studie werden die metabolischen Wirkungen eines definierten Thiaminmangels auf den Lactat-, Säuren-Basen- und Aminosäurenhaushalt unter Ruhe- und Belastungsbedingungen überprüft. In guter Korrelation zur biologischen Halbwertszeit von Thiamin und in Übereinstimmung mit Literaturangaben bedingte die Thiaminmangelkost mit einer Latenz von 17 bis 56 Tagen parallel 1. statistisch gesicherte Hyperlaktatämien, 2. gegenüber den Kontrollwerten sehr deutlich, in der Mehrzahl statistisch gesichert erhöhte Plasmakonzentrationen von Threonin, Alanin, Glutaminsäure, Glutamin, Histidin, Methionin, Taurin, Valin, Isoleucin und Leucin sowie 3. gegenüber den Kontrollwerten sehr deutlich und statistisch gesichert erniedrigte Plasmakonzentrationen von Arginin und Tryptophan. Darüber hinaus bedingte die energetische Belastung im genormten Belastungstest bei den thiaminarm ernährten Tieren parallel bedrohliche Laktazidosen, erheblich, statistisch gesichert ausgeprägtere Homöostasestörungen von Alanin, Glycin, Methionin und Arginin, sehr deutlich und statistisch signifikant erhöhte Plasmakonzentrationen von Prolin sowie statistisch signifikant abgesunkene, wieder im Bereich der Kontrollwerte liegende Plasmakonzentrationen von Taurin. Für die Mehrzahl der unter Thiaminmangel erhobenen Befunde muß als entscheidende metabolische Ursache eine hepatische Verwertungsstörung von Pyruvat angenommen werden. Störungen des Transsulfuration-Pathway und Krebs-Henseleit-Zyklus erscheinen wahrscheinlich. Die physiologische Regulation von Pyruvat, Lactat, Alanin, Serin und Tyrosin bei muskulärer Arbeit wird anhand der Kontrollwerte diskutiert.
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    European journal of nutrition 23 (1984), S. 230-236 
    ISSN: 1436-6215
    Keywords: selenium ; vegetable ; fruit ; cereals ; infant food
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Der Selengehalt der Nahrung weist große regionale Unterschiede auf. Nahrungsproben von Säuglings- und Kleinkinderkost aus Nordrhein-Westfalen wurden mit Hilfe der instrumentellen Neutronenaktivierungsanalyse untersucht und zeigten, daß der Selengehalt der hiesigen Gemüse und Früchte durchschnittlich etwa 5 ng/g beträgt. Nur Proben von Kartoffeln und Bananen weisen einen höheren Selengehalt auf. Bananen, importiert aus verschiedenen Gebieten der Erde, zeigen eine extrem hohe Schwankungsbreite mit Werten zwischen 4 und 164 ng Se/g. Fertiggerichte für Säuglinge und Kleinkinder haben einen der Kuhmilch vergleichbar niedrigen Selengehalt von durchschnittlich 24 ng/g. Außer Eiern enthalten nur Milchbreie und einige andere getreidehaltige Produkte mehr als 100 ng Se/g.
    Notes: Summary The selenium content of food exhibits great regional differences. Food samples of infants and young children from the North Rhine-Westphalia State in the Fed. Rep. of Germany were analysed by instrumental neutron activation analysis and showed that the average selenium content of local vegetable and fruit is below 5 ng/g wet weight. Only samples of potatoes and bananas exhibit higher selenium contents. The bananas, probably imported from different areas of the world, show an extremely high variance with values between 4 and 164 ng Se/g. Commercially available ready-for-use meals for infants or young children have a low Se content (median 24 ng/g) which is comparable to cow's milk. Besides eggs, cereals enriched with milk and some other cereal products contain more than 100 ng Se/g.
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    European journal of nutrition 23 (1984), S. 241-254 
    ISSN: 1436-6215
    Keywords: Verzehrsregulation ; Sättigung ; Verzehrsverhalten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Food intake in man and animals occurs as meals. Because changes in daily food intake in laboratory animals are often associated with corresponding changes in meal size, while meal frequency is less affected, food intake appears to be regulated mainly by the satiety mechanism. Meal size is controlled by feedback or satiety signals originating from the stomach, the small intestine, the liver and the fat depots. Both neural and hormonal (Bombesin?, Cholecystokinin?) feedback signals are elicited in the stomach and intestine. The feedback signals of the liver apparently originate from hepatic chemoreceptors, whose activity is modulated by certain metabolites, in particular glucose. The satiety effect of pancreatic glucagon, whose plasma concentration in the portal vein increases during a meal, also appears to be mediated by the hepatic chemoreceptors. Unmyelated nerve endings are probably the morphological substrate of the hepatic chemoreceptors. There is some evidence that circulating fat metabolites (fatty acids, ketone bodies, glycerol), whose plasma levels are related to the size of the fat depots, function as lipostatic feedback signals. The signal transfer from the gastrointestinal chemo- and mechanoreceptors and from the hepatic chemoreceptors to the central nervous system occurs mainly through vagal afferents. The first relay for this afferents is the nucleus tractus solitarii in the medulla oblongata. The next relay is at the pons, from where neurons project to the hypothalamus and other forebrain areas. The hypothalamus has important integrative functions in the control of food intake.
    Notes: Zusammenfassung Die Nahrungsaufnahme erfolgt bei Mensch und Tier in Form von Mahlzeiten. Da sich Änderungen in der täglichen Nahrungsaufnahme beim Versuchstier meist in entsprechenden Veränderungen der Mahlzeitengröße, nicht aber der Mahlzeitenfrequenz manifestieren, scheint die Nahrungsaufnahme hauptsächlich durch den Sättigungsmechanismus reguliert zu werden. Die Mahlzeitengröße wird durch vom Magen, vom Dünndarm, von der Leber und von den Fettdepots ausgehende Feedback- oder Sättigungs-Signale determiniert. Die gastralen und intestinalen Feedback-Signale scheinen dabei nervaler und hormonaler (Bombesin?, Cholezystokinin?) Natur zu sein. Die Feedback-Signale der Leber gehen offensichtlich von hepatischen Chemorezeptoren aus, deren Aktivität durch bestimmte Metabolite, insbesondere Glucose, moduliert wird. Auch die Sättigungswirkung von pankreatischem Glukagon, dessen Plasmakonzentration während der Aufnahme einer Mahlzeit in der Pfortader ansteigt, kommt wahrscheinlich unter Einschaltung der hepatischen Chemorezeptoren zustande, deren morphologisches Substrat marklose Nervenendigungen sein dürften. Als lipostatische Feedback-Signale fungieren wahrscheinlich im Blut zirkulierende Metabolite des Fettstoffwechsels (Fettsäuren, Ketonkörper, Glyzerin), deren Plasmakonzentrationen mit der Größe der Fettdepots korreliert sind. Die nervale Übertragung der von den gastrointestinalen Chemo- und Mechanorezeptoren sowie den hepatischen Chemorezeptoren ausgehenden Feedback-Signale zum Zentralnervensystem erfolgt hauptsächlich über afferente Vagusfasern. Als erste Relais-Station fungiert diesbezüglich der Nucleus tractus solitarii der Medulla oblongata, von dem aus Nervenfasern über eine Relais-Station in der Pons zum Hypothalamus und anderen Vorderhirnregionen projizieren. Dem Hypothalamus scheinen bei der Regulation der Nahrungsaufnahme wichtige integrative Funktionen zuzukommen.
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    European journal of nutrition 23 (1984), S. 291-291 
    ISSN: 1436-6215
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
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    Cellular and molecular life sciences 40 (1984), S. 1185-1188 
    ISSN: 1420-9071
    Keywords: Calmodulin ; cyclic AMP ; myosin ; protein kinase ; phosphorylation ; smooth muscle (gizzards)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In smooth muscle the Mr 20,000 light chain of myosin is phosphorylated by a calmodulin-dependent protein kinase. It consists of 2 subunits: calmodulin, an acidic protein of Mr 17,000 that binds 4 moles of Ca2+; and a larger protein of Mr circa 130,000. Activation of the kinase is dependent upon their association in the presence of Ca2+. Cyclic AMP-dependent protein kinase phosphorylation of the myosin light chain kinase occurs at 2 sites. It decreases the affinity of the kinase for calmodulin and a reduction in the rate of light chain phosphorylation occurs. The kinase has an overall asymmetric shape composed of a globular head and tail region for the skeletal muscle enzyme. Trypsin digestion of this kinase releases a fragment of Mr 36,000 from the globular region that contains the catalytic and calmodulin binding sites. Chymotrypsin digestion of the kinase from smooth muscle generates a fragment of Mr 80,000 that does not contain the calmodulin binding or cyclic AMP-dependent protein kinase phosphorylation sites. It is a Ca2+-independent form of the kinase that phosphorylates the light chain of myosin. These structural features indicate a regulatory role for the kinase in smooth muscle phosphorylation and contraction.
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    Cellular and molecular life sciences 40 (1984), S. 1366-1367 
    ISSN: 1420-9071
    Keywords: Catfish ; electroreceptors ; skin ; magnetic stimulation ; siluriform fishes ; lateral line organ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The Turkistan catfish,Glyptosternum reticulatum, has highly sensitive electroreceptors (threshold voltage gradient 1 μV/cm) that detect a voltage drop across the skin. These electroreceptors were found to be sensitive to magnetic stimulation.
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    Cellular and molecular life sciences 40 (1984), S. 1373-1375 
    ISSN: 1420-9071
    Keywords: Guinea pig ; cholesterol content ; cholesterol esterifying capacity ; cholesterol, total ; cholesterol, esterified ; acyl cholesterol acyltransferase
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    Notes: Summary In various organs of the guinea pig, the total cholesterol content of an organ was significantly correlated with the percentage of esterified cholesterol present in this organ. Cholesterol esterifying capacity was shown in most organs, with highest activities in the adrenals, the spleen and the liver. The significant correlation found between the cholesteryl ester content of an organ and its acyl cholesterol acyltransferase activity suggests a possible role of this enzyme in determining the level of the total and esterified cholesterol in a tissue.
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    Cellular and molecular life sciences 40 (1984), S. 1384-1385 
    ISSN: 1420-9071
    Keywords: Rat, female, castrated ; oestradiol ; ovariectomy ; cell proliferation ; adrenal cortex, proliferation ; DNA-synthesizing ; cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ovariectomy and subsequent treatment with ovarian hormone produces a temporary increase in DNA-synthesizing cells in the zona glomerulosa of the adrenal cortex.
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  • 21
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    Keywords: Rat liver ; liver, rat ; mitochondria ; irradiation ; 60Co ; lipid peroxidation ; succinate
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    Notes: Summary Succinate inhibits NADPH-dependent lipid peroxidation of liver mitochondria. This effect of succinate decreased 12 h after whole-body60Co-gamma irradiation, depending on the dose of irradiation.
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    Cellular and molecular life sciences 40 (1984), S. 1395-1396 
    ISSN: 1420-9071
    Keywords: Astropecten latespinosus ; starfish ; tetrodotoxin
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    Topics: Biology , Medicine
    Notes: Summary A toxin causing paralysis was detected in the starfishAstropecten latespinosus. The toxin was purified by a method consisting of charcoal treatment and chromatography on CM-Sephadex C-25 and Bio-Rex 70. The toxin was identified as tetrodotoxin by its behavior in thin-layer chromatography and electrophoresis and its1H-NMR spectrum.
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    Cellular and molecular life sciences 40 (1984), S. 1400-1401 
    ISSN: 1420-9071
    Keywords: Myoglobin ; conformational stability ; basal metabolic rate ; unfolding, free energy of
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    Notes: Summary The free energy of unfolding of several myoglobins from different animal species has been determined from their denatunation pattern by using the ligand binding model. The results indicate that no simple correlation exists between the free energy of unfolding of myoglobin and the basal metabolic rate of the animal species from which the myoglobin was isolated
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    Cellular and molecular life sciences 40 (1984), S. 1387-1388 
    ISSN: 1420-9071
    Keywords: Vestigial ; imaginal disc ; regeneration
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    Notes: Summary The development of the middle row of triple row wing bristles was examined in flies homozygous for thevestigial mutant, grown at 31°C. While bristle number, length and spacing all improved toward the wild-type condition, the mean values for both sexes were only about 10% of those of wild-type, although development in males was significantly more complete than in females. The results suggest an explanation for the apparent lack of regenerative ability in this tissue.
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    Cellular and molecular life sciences 40 (1984), S. 1396-1397 
    ISSN: 1420-9071
    Keywords: Drosophila melanogaster ; ovicidal activity ; dehydromatricaria ester ; toxicity, enhancement by UV ; polyacetylenic compounds
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    Notes: Summary Freshly laid eggs ofDrosophila melanogaster were treated with the polyacetylenic compoundcis-dehydromatricaria ester. The toxicity of the chemical was enhanced by treatments with long-wavelength ultraviolet light, and the maximum effect was observed when the UV irradiation was performed 4–5 h after the initial contact of the eggs with the chemical.
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    Cellular and molecular life sciences 40 (1984), S. 1401-1403 
    ISSN: 1420-9071
    Keywords: Protozoa, ciliate ; ciliates ; Blepharisma japonicum ; DNA, macronuclear ; 5′-methylcytosine
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary Brief report on the presence of 5′-methyl-cytosine as a minor base (0.56%) in the macronuclear DNA of the ciliate orotozoanBlepharisma japonicum. The evidence comes from electrophoresis of macronuclear DNA digested by appropriate restriction endonucleases and high-performance liquid chromatography
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  • 27
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    Cellular and molecular life sciences 40 (1984), S. 1330-1343 
    ISSN: 1420-9071
    Keywords: Malaria ; Plasmodia ; mosquito ; gametocyte ; ookinete ; oocyst ; sporozoite
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    Cellular and molecular life sciences 40 (1984), S. 195-196 
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    Notes: Summary Effect of RES-blockade on bone marrow cells was studied serially after irradiation in LPS-resistant mice. Injection of carbon particles reduced damage and accelerated recovery of marrow hemopoietic stem cells, indicating that LPS-resistant mice can react normally to RES-blockade.
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    Cellular and molecular life sciences 40 (1984), S. 1428-1429 
    ISSN: 1420-9071
    Keywords: Mice, male ; housing density ; testosterone secretion ; crowding, post-weaning
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    Notes: Summary The effect of different densities of animals per cage on basal testosterone secretion and its response to some stimuli was studied in prepuberal male mice. Mice housed with 110 or 55 cm2 of floor space per animal showed the same basal serum testosterone and hCG-induced testosterone release. Likewise, in a second experiment, the same basal serum testosterone and a similar response to acute noise stress were found in mice housed at 132, 66 or 22 cm2 of floor space per animal. These results suggest that post-weaning crowding did not affect Leydig cell function in male mice.
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    Cellular and molecular life sciences 40 (1984), S. 1433-1434 
    ISSN: 1420-9071
    Keywords: Somatic mutation ; color mosaicism ; leaf chemistry ; herbivory
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    Notes: Summary The color mosaicism was observed to correlate with selective herbivory on different leaf areas of individual leaves of the variegated sycamoreAcer pseudoplatanus. The mosaicism affected the content of nutrients and of phenolic compounds and also the mechanical properties of the different leaf areas; this offers an explanation for the observed selective feeding by insect herbivores.
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    Cellular and molecular life sciences 40 (1984), S. 157-164 
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    Cellular and molecular life sciences 40 (1984), S. 164-167 
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    Notes: Summary Immunization of mice with subcellular fractions ofC. fasciculata led to myocarditis and electrocardiographic alterations similar to those induced by immunization withT. cruzi, the etiological agent of Chagas' disease, suggesting the presence of similar cardiotoxic antigens in both trypanosomatid flagellates.
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    Cellular and molecular life sciences 40 (1984), S. 174-176 
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    Notes: Summary The time course of ATPase activity and force has been determined during contraction and relaxation in skinned (hyperpermeable) anterior byssus retractor muscle, ABRM, ofMytilus edulis and compared with corresponding measurements on skinned taenia coli of guinea-pigs. Following a calcium-induced contraction, lowering the [Ca++] to 10−8 M rapidly reduces ATPase activity within 2 min to resting levels while force declines only to about 30–50% of maximal tension within the same time. Thus slow relaxation is due to a ‘catch-like-state’ which is common to different kinds of smooth muscles and can be reduced with cAMP in ABRM and by Pi in taenia coli.
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    Cellular and molecular life sciences 40 (1984), S. 184-185 
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    Notes: Summary Quercetin was found to interact with the Ca2+-calmodulin complex, suggesting that it may act as a calmodulin antagonist in vivo to inhibit calmodulin-dependent phenomena caused by biologically active agents, including tumor promoters.
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    Notes: Summary The organophosphorus insecticides chlorpyrifos, leptophos, and phosfolan and the carbamate methomyl was found to be more toxic to larvae of a susceptible strain ofSpodoptera littoralis (Boisd.) when the posttreatment temperature was increased from 20 to 35°C. In contrast, the pyrethroids permethrin, fenvalerate, deltamethrin, cypermethrin, and flucythrinate were more toxic at 20°C than at 35°C. This effect was more pronounced in the pyrethroid-resistant strains. Evidently, resistance levels were reduced at low temperature. However, the application of piperonyl butoxide or DEF in combinations with the tested pyrethroids on R-strains resulted in reducing the effect of temperature.
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    Notes: Summary The solubilizing effect of Triton X-100 on beef heart submitochondrial particles (ETPH) has been studied under various physiological conditions. Coupled, uncoupled and azide-inhibited ETPH particles have been studied. Quantitative and qualitative differences are found in the proteins solubilized by the detergent from ETPH particles under the various conditions tested.
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    Cellular and molecular life sciences 40 (1984), S. 200-201 
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    Notes: Summary Five hybridomas secreting monoclonal antibody toE. coli L-asparaginase were isolated. These monoclonal antibodies were classified into 3 different subclasses; Ig G1 (1 clone), Ig G2 (2 clones) and Ig G3 (2 clones). One of them possessed anti-L-asparaginase neutralizing activity. Four antibodies examined demonstrated a linear Langmuir binding plot and binding affinities, with equilibrium dissociation constant (Kd) ranging between 2.5×10−9M and 6.3×10−10 M. The monoclonal antibodies should be useful probes for investigation of the enzyme activity.
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    Cellular and molecular life sciences 40 (1984), S. 208-209 
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    Notes: Summary Scarites occidentalis has 2n=41 (females 2n=42), n=19+X1X2Y. This multiple sex-chromosome system and other karyotypic characteristics suggest that this species andS. buparius share a recent common ancestor in which the sex trivalent was probably originated.
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    Cellular and molecular life sciences 40 (1984), S. 206-207 
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    Notes: Summary Intramitochondrial myelin-like structures were found in ventricular myocardial cells of the rat heart. These inclusions were found in both the first 2 postnatal weeks and in senescent stages. The origin of such myelin-like structures could be related to an age-dependent metabolic change, specifically to an inability to oxidize long-chain fatty acids described in both periods.
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    Cellular and molecular life sciences 40 (1984), S. 213-215 
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    Notes: Summary Female mice that had been made estrous through hormone implantation excreted in their urine significantly enhanced levels of n-pentyl acetate,cis-2-penten-1-yl acetate, p-toluidine, 2-heptanone, and 3 unsaturated ketones. The relationship of these volatiles to a signaling function of the estrous urine is postulated. Structural elucidations of these compounds were carried out through capillary gas chromatography/mass spectrometry and the synthesis of authentic samples.
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    Notes: Summary In a total of 29 tench (Tinca tinca L., Cyprinidae, Ostariophysi, Pisces) we operatively impaired either 1 or 2 of the 3 nerve bundles of both tractus olfactorii. Through behavioral experiments, using the fright reaction as a test, we found out which nerve bundle of the tractus olfactorius conducts the excitation elicited by the alarm substance. According to the results the conducting bundles are exclusively the lateral bundles in both tractus olfactorii.
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    Cellular and molecular life sciences 40 (1984), S. 224-226 
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    Notes: Summary Increased methylation of middle lamellar pectin in plants hinders aphids in penetrating host-plant tissue. In sorghum, a new aphid biotype has overcome this host-plant barrier by having increased pectin methylesterase activity. Results suggest that resistance in crop-plants against sap-feeding insects may possibly be manipulated by altering middle lamellar chemistry either through breeding or use of certain plant-growth regulators.
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    Cellular and molecular life sciences 40 (1984), S. 229-230 
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    Notes: Summary Cytochrome c, used as a scavenger for superoxide anion in some oxidase-like reactions of horseradish peroxidase, diminishes the conversion of the starting products to the oxidized forms. This behavior can be used as a tool for determining superoxide in the presence of peroxidases and hydrogen peroxide.
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    Cellular and molecular life sciences 40 (1984), S. 231-234 
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    Cellular and molecular life sciences 40 (1984), S. 136-142 
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    Notes: Summary The health effects of human exposure to cadmium are discussed with emphases on intake, absorption, body burden, and excretion; osteomalacia in Japan; hypertension; and proteinuria, emphysema, osteomalacia, and cancer in workers. Elevated blood pressure has not been observed as a result of excessive exposures to cadmium in Japan or the workplace. Renal tubular dysfunction and consequent proteinuria is generally accepted as the main effect following long-term, low-level exposure to cadmium. Studies of workers show that proteinuria may develop after the first year of exposure or many years after the last exposure. Proteinuria and deterioration of renal function may continue even after cessation of exposure. The immediate health significance of low-level proteinuria is still under debate. However, there is evidence that long-term renal tubular dysfunction may lead to abnormalities of calcium metabolism and osteomalacia. The few autopsy and crosssectional studies of workers do not permit conclusions to be drawn regarding the relationship between cadmium exposure and emphysema. Retrospective and historical-prospective studies are needed to settle this important question. No conclusive evidence has been published regarding cadmium-induced cancer in humans. However, there is sufficient evidence to regard cadmium as a suspect renal and prostate carcinogen. Because of equivocal results and the absence of dose-response relationships, the studies reviewed should be used with caution in making regulatory decisions and low-dose risk assessments.
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    Cellular and molecular life sciences 40 (1984), S. 271-273 
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    Notes: Summary Inoculation of human red blood cells (HRBC) into the anterior chamber of the eye (AC) of rabbits effectively stimulazed systemic antibody production. Intraocular (i.o.) inoculation was observed to be more effective a route of immunization than i.v. or i.m. inoculation of antigen. These results contradict the accepted belief that the AC is an immunologically privileged site in the body.
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    Cellular and molecular life sciences 40 (1984), S. 279-281 
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    Notes: Summary A number of self-incompatibility genotypes ofB. oleracea were analyzed by sensitive electrophoretic procedures. Members of the class of S-allele specific glycoproteins that increase in correlation with the onset of the incompatibility response were resolved into several components on SDS gels. The implications of this molecular heterogeneity are discussed in relation to S locus function.
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    Cellular and molecular life sciences 40 (1984), S. 304-306 
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    Notes: Summary A simple method is described which makes it possible to eliminate the retained eggs ofDrosophila melanogaster and to harvest a large amount of embryos at a defined stage of development. It is based upon carbon dioxide anesthesia, which brings about the expulsion of eggs remaining in the uterus.
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    Cellular and molecular life sciences 40 (1984), S. 244-246 
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    Notes: Summary Simple monosaccharides are shown to autoxidize, under physiological conditions, forming dicarbonyl compounds and hydrogen peroxide via reactive intermediates of dioxygen and carbon centred free radicals. The rate of enolisation of the substrate to form an enediol is the rate limiting step of this spontaneous process.
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    Cellular and molecular life sciences 40 (1984), S. 250-252 
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    Notes: Summary D. pseudoobscura oviposits mostly during the day with some oviposition at night. Oviposition varies from tight clustering to loose scattering from different individuals. Daily oviposition ranges from good to poor to no eggs over 24 h. Althoug this species was an excellent model for the eclosion rhythm it does not serve as a good model for continued study of the oviposition rhythm under present experimental conditions.
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    Cellular and molecular life sciences 40 (1984), S. 259-261 
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    Notes: Summary The variation of kinetic parameters with pH for the reaction catalysed by the purified 14-day embryo and adult hen heart AMP-deaminase was shown to be similar but not identical. The pH-dependence of the half-saturation constant (K0.5) is well pronounced, and the plot of pK0.5 vs pH is manifested as a bell-shaped curve for both developmental forms of the enzyme. In contrast to that, the maximum velocity of the reaction (Vmax) catalyzed by these enzymes does not change significantly in the range pH 5.6–7.4.
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    Cellular and molecular life sciences 40 (1984), S. 264-265 
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    Notes: Summary 8-Methoxypsoralen and angelicin, locally applied to the skin of the rabbit, photosensitized erythema under UV irradiation to an approximately equal extent. Incident UV irradiation was restricted mainly to 334 nm. This indicates that furocoumarin-DNA cross-links are not important in erythema induction.
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    Cellular and molecular life sciences 40 (1984), S. 267-269 
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    Notes: Summary (3H)Nitrendipine binding to the bovine tracheal muscle membrane at 25°C was rapid, saturable (Bmax=14.8±3.9fmol/mg protein) and of high affinity (Kd=0.15±0.04 nM). The rank order of Ca2+ antagonists competing for airway (3H)nitrendipine binding was nitrendipine $$ \doteqdot $$ nisoldipine $$ \doteqdot $$ nifedipine » verapamil. Cromolyn, however, neither inhibited nor increased the binding.
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    Cellular and molecular life sciences 40 (1984), S. 361-362 
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    Notes: Summary Severala-pyrones have been synthesized and investigated for their in vitro inhibitory activity usinga-chymotrypsin (a-CT), porcine pancreatic elastase, (PPE) and human leukocyte elastase (HLE). 4-Hydroxy-6-undecyl-2H-pyran-2-one4, 4-Hydroxy-6-[(1-butyl)heptyl]-2H-pyran-2-one5 and 4-Methoxy-6-[(1-butyl)heptyl]-2H-pyran-2-one6 were found to be specific inhibitors of HLE. These compounds constitute a promising new class of HLE inhibitors.
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    Cellular and molecular life sciences 40 (1984), S. 276-277 
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    Notes: Summary Purified nuclei from rat testes interstitial cells were incubated with an equimolar complex of [3H]retinoic acid and purified cellular retinoic acid-binding protein (cRABP) and with ATP. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and radiofluorographic analysis of the nuclear fractions indicated the presence of 3 highly labeled receptors for retinoic acid which were distinct from cRABP. These data demonstrate that retinoic acid binds to 3 novel nuclear acceptors of which cRABP does not appear to be a part.
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    Cellular and molecular life sciences 40 (1984), S. 285-286 
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    Notes: Summary Allium sativum (garlic) root-tip chromosomes were subjected to a C-banding procedure. In addition to the nucleolar bands reported previously in this species, bands which are telomeric or close to the telomeres have been detected in some pairs. This has allowed us to analyze the arrangement of chromosomes during interphase.
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    Cellular and molecular life sciences 40 (1984), S. 289-291 
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    Notes: Summary Follicles isolated from Alloxan-treated rabbits and incubated in vitro, accumulated more progesterone and testosterone than those from saline-treated rabbits. LH augmented the accumulation of these 2 steroids. By contrast, the estradiol response to LH stimulation by follicles from Alloxan-induced hyperglycemic rabbits was diminished when compared to follicles from saline-treated rabbits. Ovaries from hyperglycemic rabbits also appeared to have more cystic follicles.
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    Cellular and molecular life sciences 40 (1984), S. 298-299 
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    Notes: Summary Ipsilateral dorsal root potentials evoked by volleys entering lumbar and sacral segments of the cord are largest at the level of entry of afferent volleys. Contralateral potentials resulting from stimulation of any of these nerves attain maximum amplitude in lower sacral and upper caudal segments.
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    Cellular and molecular life sciences 40 (1984), S. 404-406 
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    Notes: Summary The myenteric plexus-longitudinal muscle preparation of the guinea-pig ileum offers, by its anatomical arrangement, the possibility of studying a new aspect of posttetanic potentiation (PTP); its topography. Evidence was sought and obtained that during PTP more distal junctional sites of cholinergic nerve terminals may be recruited into the transmitter secretion process.
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    Cellular and molecular life sciences 40 (1984), S. 408-408 
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    Cellular and molecular life sciences 40 (1984), S. 342-343 
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    Notes: Summary By means of an intracellular glass microelectrode, action potential changes were successfully recorded in situ from the endothelial cells of rat mesenteric lymphatics over the temperature range of 27–40°C. The frequency of action potential and the lymphatic contraction rate correlated well with temperature.
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    Cellular and molecular life sciences 40 (1984), S. 246-248 
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    Notes: Summary Five new polyoxygenated steroids, 24-methylcholesta-1,4,22E-trien-16β, 18,20ξ-triol-3-one (1), cholest-1,4,22E-trien-16β, 18,20ξ-triol-3-one (2), 24-methylcholesta-4,22E-dien-16β, 18, 20ξ-triol-3-one (3), cholesta-4,22E-dien-16β, 18,20ξ-triol-3-one (4) and 27-nor-24-methylcholesta-4,22E-dien-16β,18,20ξ-triol-3-one (5), have been found in a marine organism, the gorgonianLeptogorgia sarmentosa.
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    Cellular and molecular life sciences 40 (1984), S. 346-348 
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    Notes: Summary An increase in venous pressure in the rat tail is known to result in acute edema. Acute venous stasis edema of the rat tail was induced by applying a force-controlled banding of standard tension (200 g) proximally for a period of 6–12 h. The hemodynamic changes of acute venous stasis edema were evaluated using non-invasive plethysmography, fluorescence angiography, computer thermography and invasive radioactive microsphere techniques. It is shown here that reduction of tail circulation to 40% of the control value is followed by prolonged vascular disorder characterized by genesis of reversible edema, increased total blood flow to the tail and decreased local cutaneous blood flow, without affecting the general hemodynamics. The cutaneous circulation (decreased blood flow) seems to be principally involved in the edemogenic response, whereas the deeper vessels (hyperemia) may or may not play a determinant role in acute experimental venous stasis edema in rats.
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    Cellular and molecular life sciences 40 (1984), S. 350-351 
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    Notes: Summary Male Syrian hamsters were fed a lithogenic diet containing 7% cellulose or 4.2% pectin. After 50 days, pectin was 76% and cellulose 64% less lithogenic than the control diet. Hamsters fed the control diet for 50 days were maintained on that diet for another 50 days or fed diets containing cellulose or pectin. These was a 52% increase in gallstone incidence in hamsters continued on the control diet and a 9% increase in those on cellulose. Pectin promoted regression of gallstones (by 52%).
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    Cellular and molecular life sciences 40 (1984), S. 356-357 
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    Notes: Summary The cell wall ofNeurospora crassa was digested enzymatically and the cytosolic and the mitochondrial fractions were separated. The activity of pyruvate carboxylase (EC 6.4.1.1) was detected entirely in the cytosolic fraction. This indicates that the location of pyruvate carboxylase ofN. crassa is in the cytosol, but is not in the mitochondria; this is different from the situation in animal tissues.
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    Cellular and molecular life sciences 40 (1984), S. 458-459 
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    Notes: Summary Only 1 of 7 dogs with long-standing renovascular hypertension showed clear changes in the fundus. No distinct retinopathy was seen in the others. Ophthalmoscopy alone is thus of limited value in assessing the progress of benign hypertension in the dog.
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    Cellular and molecular life sciences 40 (1984), S. 465-466 
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    Notes: Summary Centchroman, a non-steroidal antifertility agent showed a low affinity (Kd=13.19×10−6 M) and nonsaturable binding to human plasma. Centchroman did not complete either with sex hormone binding globulin or corticosteroid binding globulin. Polyacrylamide gel electrophoresis and temperature dependent binding characteristics revealed that the protein responsible for centchroman binding to human plasma resembles albumin.
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    Cellular and molecular life sciences 40 (1984), S. 490-492 
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    Notes: Summary Transplantation of muscle minces with and without autogenous bone marrow cells was performed in the monkey. The addition of bone marrow cells markedly enhanced muscle regeneration. The findings suggest a possible clinical application of the technique.
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    Cellular and molecular life sciences 40 (1984), S. 498-500 
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    Notes: Summary A significant decrease in the activity of somatomedin-C (SM-C) was observed due to zinc deficiency. SM-C activity correlated significantly with b.wt gain and with the status of zinc in rats. The present findings provide an insight into the mechanism by which zinc promotes growth and development.
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    Cellular and molecular life sciences 40 (1984), S. 503-505 
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    Notes: Summary Fluid obtained from rat fetuses was utilized to characterize the affinity, number of binding sites, and the association and dissociation rate kinetics of the binding of estradiol and estrone to AFP. Statistical analysis demonstrated no differences when the values for the AFP-estradiol interaction were compared with those obtained for the AFP-estrone interaction. These data demonstrate that rat AFP specifically binds estradiol and estrone with a high capacity, high affinity, and similar binding kinetics.
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    Cellular and molecular life sciences 40 (1984), S. 501-502 
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    Notes: Summary Mclatonin was administered in 3 seasons to adult male mountain hares (Lepus timidus L.) kept in long or short photoperiods. Mclatonin and short photoperiods induced testis regression and fur whitening in summer and autumn but not in winter. Both treatments combined seemed to provoke an advanced onset of the refractory period.
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    Cellular and molecular life sciences 40 (1984), S. 510-512 
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    Notes: Summary 12% of night nurses surveyed claimed to have suffered from a totally incapacitating paralysis that may be related to sleep paralysis, and contribute to impaired levels of safety on the night shift. The incidence of this paralysis is shown to be age-related, largely confined to the early hours of the morning, and to increase over consecutive night shifts.
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    Cellular and molecular life sciences 40 (1984), S. 410-416 
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    Cellular and molecular life sciences 40 (1984), S. 417-422 
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    Cellular and molecular life sciences 40 (1984), S. 540-541 
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    Notes: Summary A urinary concentrating defect was induced in Munich-Wistar rats by removing the renal pelvis from 1 kidney. This defect was partially corrected by crudely simulating the actions of pelvic wall peristalsis with a mechanical system that cyclically compressed the exposed renal papilla.
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    Notes: Summary Partial recovery of fluorescence intensity after irradiation-induced fading of lipopigment autofluorescence is reported, during a subsequent period of 60 sec when each specimen was in darkness. This phenomenon may have significance for various techniques of quantitative fluorescence microscopy.
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    Cellular and molecular life sciences 40 (1984), S. 546-547 
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    Notes: Summary The composition of the defensive secretion of larvae of 8 Nematinae species is reported. 7 derivatives have been identified: benzaldehyde, (E)-2-hexenol, (E)-2-hexenal, (E)-4-oxo-2-hexenal, geranial, neral andcis, trans-dolichodial.
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    Cellular and molecular life sciences 40 (1984), S. 551-553 
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    Notes: Summary Release of Ca++, Mg++ and K+ by the carboxylic ionophore X-14547 A was studied in the mitochondrial membrane. A comparison was made with A.23187 (Calcimycin) and X.537 A (Lasalocid A) under the same experimental conditions. It was shown that in this test system X.14547 A is primarily a K+ carrier comparable with X.537 A.
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    Cellular and molecular life sciences 40 (1984), S. 566-567 
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    Notes: Summary Drosophila melanogaster is less sensitive to low concentrations of CO2 (2–20%) thanDrosophila simulans with regard to egg-to-adult mortality, duration of development and adult size measured by wing length, thorax length and dry weight. This difference may be related to the better adaptation ofD. melanogaster to alcoholic fermentation.
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    Cellular and molecular life sciences 40 (1984), S. 575-576 
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    Notes: Summary Amniotic fluid was obtained from 19-day-old rat fetuses by aspiration. Pooled samples measured at 4 different dilutions demonstrated parallelism with standard rat TSH. It is concluded that rat amniotic fluid has TSH immunoactivity.
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    Notes: Summary Alpha-terthienyl, a phototoxic thiophene derivative from species in the Asteraceae reduced feeding and growth of the phytophagous lepidopteran,Euxoa messoria, when incorporated into artificial diets at a concentration of 100 ppm. The effects of this substance were substantially enhanced by including photosensitizing near-UV radiation in the trials. The results suggest that the phototoxic properties of this secondary substance provide significant protection to the plants containing it.
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    Cellular and molecular life sciences 40 (1984), S. 579-581 
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    Notes: Summary The immunological and structural changes during the heterologous phase of experimental antibasement membrane antibody mediated disease was sequentially studied in the rat following single i.v. injections of rabbit antibodies to basement membrane antigens prepared from kidney, lung and salivary gland tissues. Although each of the anti-bodies bound strongly to GBM, structural changes were initially subtle accompanied by proteinuria and hematuria. More severe structural changes related to dose and duration of the disease did not appear for several weeks.
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    Notes: Summary A technique for measuring root activity of agricultural crops in totality in situ under field conditions has been developed for the first time. The method essentially consists of measuring the activity emanating from roots after plant injection of a gamma radiating isotope using a probe. This also helps continuous monitoring of root growth changes over time.
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    Cellular and molecular life sciences 40 (1984), S. 692-694 
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    Notes: Summary The accumulation of3H-dopamine by synaptic vesicles from rat striatum was significantly stabilized in a membrane impermeant medium. The characteristics of dopamine accumulation by striatal vesicles were quite similar to those reported for dopamine accumulation by a whole brain vesicle preparation in the same medium, and were siginificantly different from the characteristics previously reported for vesicular accumulation of norepinephrine.
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    Cellular and molecular life sciences 40 (1984), S. 700-701 
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    Notes: Summary The effects of exchange-transfusion with a proprietary gelatin-based plasma volume expander, Haemaccel, have been investigated in conscious, chronically-catheterized rats. No adverse changes in basic cardiovascular or respiratory functions occurred during the procedure although an increase in intravascular fluid sodium but not potassium concentration was observed.
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    Cellular and molecular life sciences 40 (1984), S. 410-410 
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    Cellular and molecular life sciences 40 (1984), S. 422-425 
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    Cellular and molecular life sciences 40 (1984), S. 535-537 
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    Notes: Summary The common bile duct of male Sprague-Dawley rats was cannulated with either PE 10 or PE 50 tubing. Maximal secretory rate of taurocholate averaged 389±67 (SD) and 657±115 nmoles·min−1·g liver−1 in the PE 10 and PE 50 group, respectively (p〈0.005). Maximal bile secretory pressure was significantly higher in the PE 10 group (240±28 vs 174±8 mm H20; p〈0.005). When the maximal secretory rate was exceeded, bile flow decreased in both groups but this was accompanied with a decrease in maximal bile secretory pressure in the PE 10 group only. Maximal secretory rate of bile salts is markedly influenced by experimental technique. Use of small caliber common bile duct cannulae leads to partial obstruction and decreases the apparent maximal secretory rate for taurocholate.
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    Cellular and molecular life sciences 40 (1984), S. 541-543 
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    Notes: Summary Physiological and anatomical data support the idea that in the caudal epidermis of the lugworm,Arenicola marina (L.), there are chemoreceptors detecting variations of oxygen partial pressure in the ambient water.
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    Cellular and molecular life sciences 40 (1984), S. 543-544 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two99mTc-labeling methods of human fibrinogen resulted in different complexes. This was concluded from different dissociation rates in human plasma. The dissociation could be described by a simple exponential function.
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    Cellular and molecular life sciences 40 (1984), S. 549-551 
    ISSN: 1420-9071
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    Topics: Biology , Medicine
    Notes: Summary The concentrations of β-phenylethylamine,p-tyramine,m-tyramine,m-octopamine and tryptamine in the ganglia or foot muscle ofHelix aspersa range from 〈0.6 to 11 ng/g.p-Octopamine levels are higher in ganglia (327 ng/g) than in foot muscle (4.1 ng/g). Dopamine and 5-hydroxytryptamine range from 840 to 2710 ng/g while their acid metabolites, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylacetic acid and 5-hydroxyindoleacetic acid range from 〈20 to 130 ng/g.
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    Cellular and molecular life sciences 40 (1984), S. 553-554 
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    Cellular and molecular life sciences 40 (1984), S. 559-561 
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    Topics: Biology , Medicine
    Notes: Summary After a single oral dose of cyclosporin A (82 mg/kg) in rats, tissue (kidneys liver and brain) and blood levels reached maximum values (approximately 80 μg/g and 3.5 μg/ml) between 3 and 7 h after drug administration. Drug elimination continued for at least 5 days. The 24-h urine and bile elimination was 2% for each.
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    Cellular and molecular life sciences 40 (1984), S. 561-563 
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    Topics: Biology , Medicine
    Notes: Summary Antibodies considered in this study are hemolysins synthesized by rabbits against sheep red blood cells. Ingested with the blood meal, they cross the tick midgut epithelium and retain their immunological properties in the hemolymph. During a reinfestation of rabbits, more ticks present these antibodies, and titres are generally higher than during a first infestation. Hemolysins are only found in ticks weighing 180 mg or more.
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    Cellular and molecular life sciences 40 (1984), S. 568-569 
    ISSN: 1420-9071
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    Topics: Biology , Medicine
    Notes: Summary Pollen grains ofLilium auratum which had been stored in n-pentanol, n-butanol and n-propanol for 10 years at −10°C germinated, and the generative nucleus in the pollen tube divided into 2 sperm nuclei. The resting eggs ofArtemia salina soaked in these solvents for 10 years hatched at a high rate.
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    Cellular and molecular life sciences 40 (1984), S. 573-575 
    ISSN: 1420-9071
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    Topics: Biology , Medicine
    Notes: Summary The paper presents the effects of nucleus accumbens destruction in rats. There are certain behavioral correlates (e.g., avoidance learning and dominance) which are influenced by the destruction of the nucleus accumbens, while other specific correlates are not significantly affected (namely, open field movements and competition for food). The results are discussed.
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    Cellular and molecular life sciences 40 (1984), S. 584-585 
    ISSN: 1420-9071
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    Topics: Biology , Medicine
    Notes: Summary A slow drip application of sodium pentobarbital was used to anesthetizeHelisoma duryi for surgery. The use of a 0.01 M Trizma buffer in the anesthetic medium expedites recovery and appeared to reduce swelling of the pedal sinus. These observations are attributed to the restriction of ΔpH favoring the entry of anesthetic into tissues and reducing osmotic stress experienced by the animal.
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    Cellular and molecular life sciences 40 (1984), S. 691-692 
    ISSN: 1420-9071
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    Topics: Biology , Medicine
    Notes: Summary The purpose of these experiments was to compare the contractile response to ACh of stomach fundal strips from hypertensive (SHR) and normotensive (WKY) rats during the development of hypertension. The results indicate that the reactivity to ACh is the same in fundal strips from young SHR and WKY rats; however, with maturation strips from WKY rats undergo a reduction in responsiveness which does not occur in the SHR. Therefore, strips from older SHR rats are more reactive to ACh than are those from age matched WKY rats.
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    Cellular and molecular life sciences 40 (1984), S. 697-699 
    ISSN: 1420-9071
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    Topics: Biology , Medicine
    Notes: Summary Platelet activating factor (PAF) induces a biphasic effect on guinea-pig papillary muscle: 1. a transient positive inotropic effect preceded by an increase in action potential duration (APD); 2. a marked negative effect on inotropism and on APD. Since Ca++ slow action potentials were initially enhanced by PAF and then markedly depressed, it is suggested that PAF specifically interferes with the Ca++ slow channel.
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