ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Glucosinolates
;
Rapseed-mustard
;
Brassica
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A simple, economical and efficient HPLC method has been developed for the separation and determination of the individual glucosinolates in rapessed and mustard. The method involves single-step extraction of glucosinolates with boiling water and separation of the individual glucosinolates on a Novapack RP-18 column (3.9 mm ×150mm) with 0.2 M ammonium sulphate as mobile phase. Peaks were monitored at 229 nm. All major glucosinolates could be eluted within 10 min. The method proved effective for routine analysis of glucosinolates.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02467557
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