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Identification of calcium antagonist receptor binding sites using (3H)nitrendipine in bovine tracheal smooth muscle membranes

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Summary

(3H)Nitrendipine binding to the bovine tracheal muscle membrane at 25°C was rapid, saturable (Bmax=14.8±3.9fmol/mg protein) and of high affinity (Kd=0.15±0.04 nM). The rank order of Ca2+ antagonists competing for airway (3H)nitrendipine binding was nitrendipine\( \doteqdot \) nisoldipine\( \doteqdot \) nifedipine » verapamil. Cromolyn, however, neither inhibited nor increased the binding.

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Authors and Affiliations

  1. Department of Pharmacology, National Yang-Ming Medical College, Taipei, Taiwan, People’s Republic of China

    J. B. Cheng, A. Bewtra & R. G. Townley

  2. Allergic Disease Center and Departments of Medicine and Pharmacology, Creighton University School of Medicine, 68178, Omaha, Nebraska, USA

    J. B. Cheng, A. Bewtra & R. G. Townley

Authors
  1. J. B. Cheng
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  2. A. Bewtra
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  3. R. G. Townley
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Additional information

J.B.C. is a visiting associate professor at the NYMMC. We thank Ms. Pang-jang Chang for technical assistance. This work is supported by a grant (NSC 72-0412-BO10-R20) from the National Science Council, ROC. To whom reprint requests should be addressed: Allergic Disease Center, Creighton University School of Medicine, Omaha, Nebraska 68178.

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Cheng, J.B., Bewtra, A. & Townley, R.G. Identification of calcium antagonist receptor binding sites using (3H)nitrendipine in bovine tracheal smooth muscle membranes. Experientia 40, 267–269 (1984). https://doi.org/10.1007/BF01947575

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