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  • Lepidoptera  (146)
  • Yeast
  • Springer  (219)
  • Cell Press
  • Elsevier
  • Institute of Physics
  • 1980-1984  (219)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Sex attractants for the fruit-attacking noctuids Orthosia incerta and Orthosia cruda (1984)
    Springer
    Entomologia experimentalis et applicata 36 (1984), S. 15-16 
    Details
    ISSN: 1570-7458
    Keywords: sex attractants ; Lepidoptera ; Noctuidae ; Orthosia incerta ; Orthosia cruda ; fruit pest ; Z9-14:Ac ; Z9-14:Ald ; Z11-16:Ac ; Z11-16:Ald
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00345198
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  • 2
    Electronic Resource
    Electronic Resource
    Factors affecting the dispersive behaviour of larvae of an Australian geometrid moth (1984)
    Springer
    Entomologia experimentalis et applicata 35 (1984), S. 159-167 
    Details
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Geometridae ; Ectropis excursaria ; larval dispersal ; colour polymorphism ; phototaxis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Acht Experimente wurden durchgeführt, um einige der Faktoren zu studieren, die das Wanderverhalten der Larven (Raupen) eines polyphagen Geometriden (Ectropis excursaria (Guenée)) beein-flussen. 1. Larven sind positiv phototaktisch. Die positive Phototaxis ist negativ mit Fasten, Alter und Populationsdichte korreliert. Bei hohen Temperaturen ist sie nicht mehr nachweisbar. 2. Das Wanderverhalten der Larven wird durch die Populationsdichte beeinflußt, wodurch annähernd eine konstante Dichte erhalten bleibt. Das Verhalten der individuellen Larven is dabei nicht statistisch homogen. Es gibt ‘Wanderer’ und ‘Nicht-Wanderer’. 3. Diese Verhaltensunderschiede stehen möglicherweise im Zusammenhang mit physiologischen und morphologischen Faktoren, die den individuellen Fortpflanzungserfolg und das überleben beeinflussen können; ‘Wanderer’ sind dunkler, entwickeln sich schneller und das Gewicht ihrer Puppen ist niedriger als das der ‘Nicht-Wanderer’. 4. Eine der larvalen Farbvarianten zeigte eine Präferenz für einen von zwei angebotenen Hintergründen.
    Notes: Abstract The dispersive behaviour of larvae of a polyphagous, wide-spread geometrid (Ectropis excursaria (Guenée)) was studied by examining responses to environmental and endogenous variables. It was found that differences in behaviour can be affected by environmental factors such as light, temperature, density, and plant background as well as some physiological and morphological features. The implications of these relationships are discussed as adaptative strategies to a varying environment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217535
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  • 3
    Electronic Resource
    Electronic Resource
    Foodplant selection and induction of feeding preference among host and non-host plants in larvae of the tobacco hornworm Manduca sexta (1984)
    Springer
    Entomologia experimentalis et applicata 35 (1984), S. 177-193 
    Details
    ISSN: 1570-7458
    Keywords: induction of feeding preference ; host plants ; non-host plants ; Manduca sexta ; Sphingidae ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Vingt-deux espèces de plantes, dont 10 planteshôtes (Solanées), ont été testés comme plantes alimentaires pour des chenilles de ler stade de Manduca sexta. Sur cet ensemble, seulement 12 plantes (dont 9 plantes hôtes) induisaient la prise de nourriture et permettaient la croissance jusqu'au 5ème stade. La diversité des résultats suggère que les plantes pouvaient être classées en hôtes, non-hôtes acceptables et non-hôtes refusés. En utilisant le test du choix alimentaire préférentiel entre deux rondelles de feuilles, les chenilles néonates de ler stade ont préféré nettement les plantes-hôtes aux autres. Cette préférence initiale pour les plantes-hôtes était préservée quand les cheniles étaient élevées sur plantes-hôtes, mais devenait moins nette ou disparaissait pour des chenilles élevées sur d'autres plantes acceptées. Ainsi l'oligophagie ches M. sexta n'est pas induite, mais doit être héritée. Les chenilles néonates, aussi bien que celles de 5ème stade, présentent des préférences hiérarchisées parmi les plantes hôtes ou non. La seule frontiere nette observée était entre espèces de plantes acceptables ou non. Les hiérarchies préférentielles des chenilles du 5ème stade différaient légèrement lors-qu'elles avaient été élevées sur deux plantes-hôtes différentes. La différence essentielle était l'observation d'une préférence accrue pour l'espèce ayant servi à l'élevage, mais deux autres plantes-hôtes changaient aussi de position hiérarchique. La cause de ces changements de préférence a été approfondie, les chenilles étant élevées sur des feuilles de chaque espèce acceptable (hôte ou non). Leurs préférences alimentaires ont été définies en utilisant des combinaisons diverses (hôte x hôte, hôte x non-hôte acceptable, non-hôte acceptable x non-hôte acceptable). L'induction de la préférence alimentaires a été obtenue dans ces trois associations. Ceci montre que l'induction des choix alimentaires chez M. sexta n'est pas limitée aux plantes-hôtes. Le degré d'induction de la préférence alimentaire variait de très fort à indécelable; il dépendait de l'association examinée. La source de la variabilité de cette induction a été examinée en fonction de la relation entre la force de l'induction et les rapports taxonomiques des plantes associées. La relation obervée était inversée pour M. sexta. L'examen des données de la littérature ont révélé une relation du même type pour les autres espèces de Lépidoptères.
    Notes: Abstract Ten host plant (Solanaceae) and twelve non-host plant species were tested as foodplants for first instar larvae of the tobacco hornworm, Manduca sexta. Only nine host and three non-host plant species elicited feeding and supported growth up to fifth instar. The range of acceptability suggested that plants be divided into hosts, acceptable non-hosts, and unacceptable non-hosts. Using the two-choice feeding preference test we found that the initial preference for hosts was preserved when larvae were reared on hosts, but was less strong or absent for larvae reared on acceptable non-hosts. Thus oligophagy in the tobacco hornworm is not induced, but must be inherited. Newly-hatched first instar larvae and fifth instar larvae showed a preference hierarchy among both hosts and non-hosts. Fifth instar larvae reared separately on two different host species showed slightly different preference hierarchies among hosts. The preference for the rearing plant was increased and also two other host species changed positions in hierarchies. Feeding preferences of larvae reared on hosts or acceptable non-hosts were determined using plant combinations of host vs. host, host vs. acceptable non-host, and acceptable non-host vs. acceptable non-host. Induction of feeding preference was found in all three of these categories. This shows that induction of feeding preference in the tobacco hornworm is not restricted to host plant species. The degree to which feeding preferences were induced ranged from very strong to undetectable and dependend on the plant species paired. The strength of induction in the tobacco hornworm was found to correlate inversely with taxonomic relatedness of the plant species paired. Analysis of induction data from the literature revealed a similar correlation for other lepidopteran species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217537
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  • 4
    Electronic Resource
    Electronic Resource
    Comparison of gravimetry and planimetry in determining dry matter budgets for three species of phytophagous lepidopteran larvae (1984)
    Springer
    Entomologia experimentalis et applicata 35 (1984), S. 255-261 
    Details
    ISSN: 1570-7458
    Keywords: method ; dry matter ; budget ; Lepidoptera ; phytophagous ; gravimetry ; area ; accuracy ; precision
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les budgets en matière sèche consommée par des lépidoptères ont été comparés par les méthodes gravimétrique et planimétrique. La méthode gravimétrique est basée sur le rapport poids sec/poids frais de feuilles consommées par les chenilles. Avec la méthode planimétrique, la quantité d'aliment proposée aux chenilles est déterminée par les tracés de la surface des feuilles et le contenu de matière sèche par unité de surface des feuilles. La méthode de planimétrie permet l'utilisation de plus grands rameaux de feuilles et de cages d'élevage extérieures en gaze. Il n'y avait pas de différence appréciable dans les éléments du budget (croissance, ingestion et déjection), ni aucune différence dans la variabilité observée des données attribuable à la méthode utilisée. Cependant, la variabilité attendue d'après la précision des mesures avec la méthode gravimétrique est inférieure à celle de la méthode planimétrique. est inférieure à celle de la méthode planimétrique. Des éléments expérimentaux, inhérents à la méthode gravimétrique, introduisent une variabilité dans les mesures que l'on n'a pas avec la méthode planimétrique. 30–60% de la variabilité dans la consommation ont été attribués à des paramètres internes à la chenille, même quand elles provenaient toutes de la même ooplaque.
    Notes: Abstract Gravimetric and a combination areal-gravimetric methods for determining dry matter budgets for leaf eating Lepidoptera were compared. The gravimetric method is based on dry weight/live weight ratios of the leaves fed to the larvae. In the areal-gravimetric method, the quantity of food offered to the larvae is determined from the area of leaf tracings and the dry matter content per unit area of the leaves. The areal-gravimetric method permits the use of larger leaf sprays and an open, gauze enclosed rearing chamber. There were no consistent differences in budget factors (growth, ingestion or egestion), nor were there any differences in the observed variability of the data attributable to the method used. However, the expected variability based on instrument precision for the gravimetric method is less than for the areal-gravimetric method. Experimental factors inherent in the gravimetric method introduce variability to the measurements that are not present in the areal method. Thirty to 60% of the variability in budget factors was attributed to intrinsic properties of the larvae, even though the larvae were taken from the same egg masses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369141
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  • 5
    Electronic Resource
    Electronic Resource
    Formation of intergeneric hybrids of yeast by protoplast fusion of Yarrowia and Kluyveromyces species (1984)
    Springer
    Current genetics 8 (1984), S. 49-55 
    Details
    ISSN: 1432-0983
    Keywords: Protoplast fusion ; Yeast ; Yarrowia lipolytica ; Kluyveromyces lactis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Prototrophic hybrids have been obtained by the fusion of auxotrophic haploid strains of the two yeasts Yarrowia (Saccharomycopsis) lipolytica and Kluyveromyces lactis. The hybrid fusants had a colonial morphology intermediate between that of the two parent strains, were uninucleate, and contained an approximately diploid amount of DNA per cell. The growth rates of all the fusants on a minimal glucose medium were slower than those of the two parents. Two of the fusants studied could utilise a novel range of carbon sources. All of these data suggested that the hybrids contained a diploid nucleus formed by the fusion of the two haploid parental nuclei. However, analytical CsCl density gradient centrifugation demonstrated that the nuclear DNA of the fusants was derived almost entirely from the Y. lipolytica parent. Moreover, an examination of the protein constitution of the fusants by two-dimensional gel electrophoresis showed that their protein patterns were indistinguishable from that of Y. lipolytica. Two possible mechanisms for the formation of a diploid nucleus containing DNA derived almost entirely from one of the haploid parents are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405432
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  • 6
    Electronic Resource
    Electronic Resource
    Nuclear suppressors of mitochondrial chloramphenicol resistance in Baker's yeast: their use for the isolation of novel mutants (1984)
    Springer
    Current genetics 8 (1984), S. 121-126 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial DNA ; Antibiotic resistance mutations ; Suppressor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Strains that are genotypically sensitive to chloramphenicol and also contain one of the nuclear suppressors of mitochondrial chloramphenicol resistance (Waxman et al. 1979) were constructed. A manganese mutagenesis on such a strain produced chloramphenicol resistant mutants, most of which resulted from mutations in nuclear genes. These mutants may be either dominant or recessive, and they probably do not code for membrane proteins. The few mitochondrial mutants fall into several classes, but all result from mutations in the 21S rRNA gene. The suppressor allele effectively prevents the appearance of the most common group of mitochondrial mutants (those that map at cap1), and thereby enhances the selection of novel mutants in the region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420230
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  • 7
    Electronic Resource
    Electronic Resource
    Antisuppression of class I suppressors in an isopentenylated-transfer RNA deficient mutant of Saccharomyces cerevisiae (1984)
    Springer
    Current genetics 8 (1984), S. 29-32 
    Details
    ISSN: 1432-0983
    Keywords: Antisuppression ; Suppression ; tRNA ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of a previously isolated antisuppressor mutation from bakers' yeast, that reduced the efficiency of the tyrosine-inserting ochre suppressor, SUP7-o, on other tyrosine-inserting ochre suppressors has been determined. As expected, the antisuppressor mutation, mod5-1, restricted the capacity of all eight tyrosine-inserting ochre suppressors to suppress nonsense mutations. Based on the suppression of five ochre alleles in the presence of mod5, the eight class I suppressors can be grouped into three subclasses. The most efficient subclass had only one member, SUP4-o. Members of the second group included SUP2-o, SUP3-o, SUP7-o, and SUP8-o. The third and least efficient subclass included SUP5-o, SUP6-o, and SUP1 1-o. These differences in efficiencies are a function of the relative expression of the eight genes encoding tRNATYR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405428
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  • 8
    Electronic Resource
    Electronic Resource
    A revised chromosome map of the fission yeast Schizosaccharomyces pombe (1984)
    Springer
    Current genetics 8 (1984), S. 85-92 
    Details
    ISSN: 1432-0983
    Keywords: Chromosome map ; Yeast ; Schizosaccharomyces pombe ; Gene conversion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genetic map of the nuclear genome of the fission yeast Schizosaccharomyces pombe has been extended by mitotic and meiotic mapping data. A total of 158 markers are now assigned to the three linkage groups known in this organism, and 118 of them have been located on the corresponding chromosome map. Chromosome II and III each consist of one linkage group. There is some indication that the two large fragments which define chromosome I are meiotically linked, but the linkage observed is significant at the P = 0.05 level only. The length of the map is at least 1,700 map units, corresponding to an average of about 8 kilobases per map unit. The latter figure is comparable to the one obtained for intragenic recombination in the sup3 gene (Hofer et al. 1979). The basic frequency of gene conversion as measured for 21 genes varies according to a distribution of Poisson (with a modal value of 0.6% conversion per meiosis and per gene), in sharp contrast with Saccharomyces cerevisiae (Fogel et al. 1980) and Ascobolus immersus (Nicolas 1979). This may reflect the rarity of gene or region-specific rec alleles in S. pombe and may be related to the homothallism of this organism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420223
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  • 9
    Electronic Resource
    Electronic Resource
    Hygromycin B resistance as dominant selectable marker in yeast (1984)
    Springer
    Current genetics 8 (1984), S. 353-358 
    Details
    ISSN: 1432-0983
    Keywords: Hygromycin B ; Yeast ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Saccharomyces cerevisiae is normally sensitive to the drug hygromycin B; a hygromycin B concentration of 200 µg/ml in agar plates is sufficient to completely inhibit growth. We constructed yeast-E. coli bifunctional plasmids which confer hygromycin B resistance to Saccharomyces cerevisiae. Promoters and amino terminal coding regions of a heat shock gene, a heat shock cognate gene, and the phosphoglycerate kinase gene from yeast were fused to a bacterial hygromycin B resistance gene. In all three cases, yeast cells containing plasmids with the hybrid hygromycin B resistance gene were resistant to high levels of the drug. Yeast cells containing these plasmids can also be directly selected after transformation by using hygromycin B. The intact bacterial hygromycin B resistance gene and the kanamycin resistance gene from Tn903 were also tested in yeast for their ability to confer resistance to hygromycin B and G418. The intact bacterial genes were not effective in conferring drug resistance to yeast cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419824
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  • 10
    Electronic Resource
    Electronic Resource
    Sensitivity to ethidium bromide during meiosis in Saccharomyces cerevisiae (1984)
    Springer
    Current genetics 8 (1984), S. 69-76 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Ethidium bromide ; Meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ethidium bromide was found to inhibit nuclear and mitochondrial DNA synthesis during meiosis which resulted in the inhibition of meiotic gene conversion and sporulation and was also lethal. Protection from the effects of ethidium bromide on meiotic gene conversion and survival was found to coincide with DNA synthesis, but it is possible that protection from sporulation inhibition occurs only later in meiosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405434
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  • 11
    Electronic Resource
    Electronic Resource
    Plasmid cloning and expression of the E. coli polA + gene in S. cerevisiae (1984)
    Springer
    Current genetics 8 (1984), S. 333-340 
    Details
    ISSN: 1432-0983
    Keywords: polA+ ; DNA polymerase I ; Cloning ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The E. coli polA + gene has been subcloned from a specialised λ transducing phage onto a low copy number plasmid. Plasmid-encoded DNA polymerase I was synthesised at 2 to 3 times the wild-type E. coli level, and was biochemically indistinguishable from chromosomally-encoded protein. It was able to counteract the radio sensitivity of polA1, polAex1, polAex2 and polA12 mutants, but no complementation of polA107 mutants occurred, even though the plasmid polA+ gene was expressed. S. cerevisiae ars-1 or 2 μ replicative sequences were introduced into the polA+ plasmid. Transformation of yeast with these constructs increased total DNA polymerase levels 2–20 times, depending upon assay conditions. The additional activity was discriminated from yeast DNA polymerases by its ability to use low concentrations of substrate, by its resistance to chemical inhibition, and by co-electrophoresis with pure DNA polymerase I and its proteolytic fragments. The polA+ gene was expressed in yeast without the aid of yeast promotor sequences. However, deletion of cloned DNA more than 99 base pairs in front of the structural gene prevented expression in yeast but not in E. coli, indicating that the two organisms use different sequences for expression of the plasmid polA+ gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419821
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  • 12
    Electronic Resource
    Electronic Resource
    Expression of the cloned endo-1,3-1,4-β-glucanase gene of Bacillus subtilis in Saccharomyces cerevisiae (1984)
    Springer
    Current genetics 8 (1984), S. 471-475 
    Details
    ISSN: 1432-0983
    Keywords: β-Glucanase ; Expression ; Heterologous DNA ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A cloned endo-1,3-1,4-β-glucanase gene from the Gram-positive bacterium B. subtilis has been located by deletion analysis on a 1.4 kb PvuI-ClaI DNA fragment. This gene has been sub-cloned in the yeast LEU2 vector pJDB207 to produce a hybrid plasmid designated pEHB9. pEHB9 has been transformed to S. cerevisiae and shown to direct the synthesis of an endo-1,3-1,4-β-glucanase in yeast. The β-glucanase activity was low and could only be detected in crude cell extracts of yeast harbouring pEHB9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433914
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  • 13
    Electronic Resource
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    Isolation and characterization of an uncoupler-resistant mutant of Saccharomyces cerevisiae (1984)
    Springer
    Current genetics 8 (1984), S. 507-516 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Mutant ; Uncoupler ; Resistance ; Permeability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary One mutant resistant to carbonylcyanide m-chlorophenylhydrazone (CCCP), an uncoupler of oxidative phosphorylation, was isolated in Saccharomyces cerevisiae. Genetic analysis showed that a single nuclear gene is responsible for increased resistance; this gene was dominant. The mutant showed cross-resistance or collateral sensitivity to several chemically-unrelated inhibitors (cycloheximide, dinitrophenol, tributhyltin chloride, chloramphenicol). The resistance of the mutant is related to a decreased uptake of CCCP which is not expressed in glucose-starved cells. It was shown that glucose induced a CCCP efflux which was more efficient in the mutant than in the wild-type cells. This effect was correlated to a greater acidification of the internal pH by glucose addition in the mutant cells. It was proposed that resistance was not due to a change of permeability of the plasmic membrane itself but to the change of internal pH which determines the extent of accumulation of weak acids or bases.
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    URL: http://dx.doi.org/10.1007/BF00410437
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  • 14
    Electronic Resource
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    A non-Mendelian factor, [eta+], causes lethality of yeast omnipotent-suppressor strains (1984)
    Springer
    Current genetics 8 (1984), S. 567-573 
    Details
    ISSN: 1432-0983
    Keywords: Non-Mendelian ; Yeast ; Suppressor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Omnipotent suppressors cause translational ambiguity and have been associated with poor growth and inviability. We now report that a non-Mendelian element, [eta+], causes this inviability. In [eta−] strains the suppressors are not inviable. The [eta+] genetic element segregates to about 70% of the meiotic progeny, although almost all of the spores probably have the [eta+] phenotype for the first few divisions. Growth on 5 mM guanidine hydrochloride efficiently causes [eta+] strains to become [eta−]. The [eta+] factor has many similarities with the previously described [psi+] factor (Cox 1965, 1971). However, [eta+] and [psi+] differ in their patterns of inheritance, and by the fact that [psi+] affects ochre specific and not omnipotent suppressors, while the converse is true of [eta+].
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    URL: http://dx.doi.org/10.1007/BF00395701
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  • 15
    Electronic Resource
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    Cloning of a yeast dihydrofolate reductase gene in Escherichia coli (1984)
    Springer
    Current genetics 8 (1984), S. 265-270 
    Details
    ISSN: 1432-0983
    Keywords: DHF Reductase ; Cloning ; Trimethoprim ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The dihydrofolate reductase gene of Saccharomyces cerevisiae has been isolated by selection of trimethoprim resistant Escherichia coli transformed with a gene bank of yeast DNA in plasmid pBR322. From 9.2 kilobase pair BamHI DNA fragment this gene has been localized to a 1.76 kb fragment, the restriction map of which appears different from those reported for the E. coli and the mouse dihydrofolate reductase genes. The enzyme encoded by the chimeric plasmid was established as yeast dihydrofolate reductase by its sensitivity to antifolates in vivo through growth studies and in vitro by enzyme assay. Since, the expression of this gene occurs independent of its orientation within the chimeric plasmid, the 1.76 kb fragment may contain functional regulatory sequences in addition to the structural sequences for yeast dihydrofolate reductase.
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    URL: http://dx.doi.org/10.1007/BF00419723
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  • 16
    Electronic Resource
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    The primary structure of a gene encoding yeast ribosomal protein L34 (1984)
    Springer
    Current genetics 9 (1984), S. 47-52 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Ribosomal protein gene ; Sequence analysis ; Northern blot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sequence analysis revealed that a gene coding for yeast ribosomal protein L34 comprises an amino acid coding region of 339 nucleotides which is interrupted by an intron after the 19th codon. Like for other yeast ribosomal protein genes analyzed thus far a strong codon bias was observed. The flanking and intervening sequences of this gene encoding L34 show several elements that are conserved in a number of split ribosomal protein genes in yeast. Northern blot analysis using an intron-specific probe demonstrated that the sequenced gene copy coding for L34 is transcribed in vivo.
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    URL: http://dx.doi.org/10.1007/BF00396203
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  • 17
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    Further evidence of a disparity between conversion and restoration in the his1 locus of Saccharomyces cerevisiae (1984)
    Springer
    Current genetics 8 (1984), S. 23-28 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Recombination ; Conversion ; Restoration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Heteroduplex repair models of recombination predict that restoration of the parental genotype from heteroduplex will have the same frequency as conversion to the genotype of the homologue. We have reported previously (Savage and Hastings 1981) that the proportion of intragenic reciprocal events in the his1 locus of yeast is too low for this expectation. In this study, two classes of recombination event involving longer lengths of his1 are compared in a series of crosses using a constant right-hand marker. This comparison gives a value for conversion: restoration for the left-hand markers which is independant of the method used before. The values obtained by the two methods are significantly correlated, confirming that there is a disparity in the ratio of conversion to restoration, and that this disparity is seen as a deficiency of restoration for five alleles of his1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405427
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  • 18
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    Effect of erythromycin upon the protein pattern of heat shocked S. cerevisiae (1984)
    Springer
    Current genetics 8 (1984), S. 429-437 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Heat-shock response ; Mitochondrial inhibition ; 2D electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Conventional and two dimensional (2D) electrophoresis on ultrathin horizontal slab gels shows that heat shock proteins are synthesized and heat stroke proteins are curtailed after the transfer of Saccharomyces cerevisiae strain Z270 from 23 °C to 37 °C. Upon addition of the mitochondrial translation inhibitor erythromycin to cell cultures which incorporated labelled methionine at 23 °C, and after the transfer to 37 °C, we have shown that: a) in extracts of cells labelled at 23 °C, translational products sensitive to erythromycin could be observed on 2D gels; the synthesis of some of these proteins was enhanced, whereas the synthesis of some others declined when the labelling was carried out 20 min after the transfer to 37 °C; b) there are heat shock proteins whose induction at 37 °C was prevented by erythromycin; c) labelling of a number of proteins became weaker at 37 °C, but not at 23 °C, when this was done in the presence of erythromycin; d) two proteins were detectable only in samples labelled at 37 °C in the presence of erythromycin.
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    Mitochondrial and nuclear mitoribosomal suppressors that enable misreading of ochre codons in yeast mitochondria (1984)
    Springer
    Current genetics 9 (1984), S. 11-19 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial ochre mutations ; Specificity of suppressors ; Mitoribosomal misreading ; Intron-encoded maturases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe studies on the action spectra of the mitochondrial suppressor mim3-1 and the three alleles of nuclear suppressor nam3. Their specificity of action was tested on 516 mit − mutations located in different mitochondrial genes. The degree of suppression was quantified by the extent of cytochrome oxidase and cytochrome b synthesis. We show that the four suppressors are allele-specific gene-nonspecific informational suppressors. They would act by changing the structure of the small mitoribosomal subunit which would decrease fidelity of translation enabling misreading of some but not all ochre codons. The implications of the results on the role of intron encoded maturases are discussed.
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    URL: http://dx.doi.org/10.1007/BF00396199
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  • 20
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    Mitochondrial and nuclear mitoribosomal suppressors that enable misreading of ochre codons in yeast mitochondria (1984)
    Springer
    Current genetics 9 (1984), S. 1-10 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial ochre mutations ; Nuclear informational suppressors ; Mitochondrial informational suppressors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A systematic search for suppressors of mutations which cause a deficiency in the splicing of mitochondrial RNA has been undertaken. These splicing mutations were localized in the mRNA-maturase coding sequence of the second intron of the cob-boxgene, i.e. in the box3locus. A total of 953 revertants (mostly spontaneous in origin) were isolated and their genetic nature (nuclear vs. mitochondrial) and phenotype characterized. Most revertants were mitochondrially determined and displayed a wild-type phenotype. A mitochondrial suppressor unlinked with the box3 −target mutation was uncovered among the revertants displaying a pseudo-wild phenotype: out of 26 revertants analyzed, derived from 7 different box3− mutants only one such suppressor mutation mim3-1 was found. It was localized by rho− deletion mapping in the region between the oxi2 and oxi3 gene, within (or in the vicinity) the gene specifying the 15S ribosomal RNA. Nuclear suppressors were isolated from seven different box3 −mutants. All were recessive and had a pseudo-wild phenotype. Three such suppressors nam3-1, nam3-2 and nam3-3 were investigated more extensively. Tetrad analysis has shown that they are alleles of the same nuclear locus NAM3 and mitotic analysis has shown that they do not segregate mitotically.
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  • 21
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    Phosphate uptake in the yeast Candida tropicalis: purification of phosphate-binding protein and investigations about its role in phosphate uptake (1984)
    Springer
    Archives of microbiology 137 (1984), S. 215-219 
    Details
    ISSN: 1432-072X
    Keywords: Yeast ; Phosphate uptake ; Phosphate-binding protein ; Anti-phosphate-binding protein antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The purification of a phosphate-binding protein (PiBP2) by immunoadsorption is described. The entire anti phosphate-binding protein 2 antibodies as well as the Fab fragments obtained from these antibodies inhibit Pi uptake by whole cells. The inhibition is a mixed type of inhibition (V m and K m are affected). These results should be regarded as a possible involvement of phosphate-binding protein 2 in Pi uptake. The binding of 125I-labelled fragments prepared from anti phosphate-binding protein 2 antibodies to whole cells, to shocked cells and to protoplasts has been investigated. The results confirm the release of phosphate-binding protein by osmotic shock and during protoplast formation. From these findings, a cell-wall localisation, near the cell surface of the phosphate-binding protein should be proposed.
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    Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2 (1984)
    Springer
    Archives of microbiology 137 (1984), S. 357-361 
    Details
    ISSN: 1432-072X
    Keywords: Yeast ; Saccharomyces cerevisiae ; Killer toxin ; Extracellular glycoprotein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were recognized. The culture filtrate of Pichia and Candida showed a much smaller activity than the strains of Saccharomyces. Extracellular killer toxins of 3 types of Saccharomyces were concentrated and partially purified. The pH optimum and the isoelectric point were determined. The killer toxins of S. cerevisiae strain NCYC 738, strain 399 and strain 28 were glycoproteins and had a molecular weight of Mr=16,000. The amino acid composition of the toxin type K2 of S. cerevisiae strain 399 was determined and compared with the composition of two other toxins.
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    Molecular analysis of inorganic nitrogen assimilation in yeasts (1984)
    Springer
    Archives of microbiology 138 (1984), S. 183-186 
    Details
    ISSN: 1432-072X
    Keywords: Yeast ; Nitrogen assimilation ; Nitrate reductase ; Nitrite reductase ; Candida utilis ; Food yeast ; Nitrate reduction ; Nitrogenous oxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Assimilation of nitrate and various other inorganic nitrogen compounds by different yeasts was investigated. Nitrate, nitrite, hydroxylamine, hydrazine, ammonium sulphate, urea and L-asparagine were tested as sole sources of nitrogen for the growth of Candida albicans, C. pelliculosa, Debaryomyces hansenii, Saccharomyces cerevisiae, C. tropicalis, and C. utilis. Ammonium sulphate and L-asparagine supported the growth of all the yeasts tested except D. hansenii while hydroxylamine and hydrazine failed to support the growth of any. Nitrate and nitrite were assimilated only by C. utilis. Nitrate utilization by C. utilis was also accompanied by the enzymatic activities of NAD(P)H: nitrate oxidoreductase (EC 1.6.6.2) and NAD(P)H: nitrite oxidoreductase (EC 1.6.6.4), but not reduced methyl viologen-or FAD-nitrate oxidoreductases (EC 1.7.99.4). It is demonstrated here that nitrate and nitrite reductase activities are responsible for the ability of C. utilis to assimilate primary nitrogen.
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    Effect of chloroquine on proteolytic processes and energy metabolism in yeast (1984)
    Springer
    Archives of microbiology 137 (1984), S. 104-108 
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    ISSN: 1432-072X
    Keywords: Chloroquine ; Yeast ; Proteolysis ; ATP hydrolysis ; Glucose consumption ; Ethanol formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In yeast cells, degradation of cellular proteins was inhibited by addition of chloroquine to the medium as shown by a decrease of the release of trichloroacetic acid-soluble radioactivity from prelabelled cell protein. Penetration of chloroquine into the cells was strongly enhanced with increasing pH value of the medium. The concentration in the cells reached 5–14 times that in the medium of pH 8.0. Fluorescence microscopy showed that chloroquine was concentrated in the vacuoles of the cells. Chloroquine, at concentrations attained in the cells, inhibited the activities of vacuolar proteinases in vitro. Furthermore, chloroquine caused a rapid and drastic decrease of the ATP content of the cells and prevented the fermentation of glucose and formation of ethanol under aerobic conditions.
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    Isolation, and biochemical and biological characterization of an a-mating-type-specific glycoprotein responsible for sexual agglutination from the cytoplasm of a-cells, in the yeast Saccharomyces cerevisiae (1984)
    Springer
    Archives of microbiology 140 (1984), S. 113-119 
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    ISSN: 1432-072X
    Keywords: Agglutination substance ; Cell-cell recognition ; Glycoprotein ; Mating ; Saccharomyces cerevisiae ; Sexual agglutinability ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An a-mating-type-specific substance responsible for sexual agglutination was purified to 397-times in specific activity (units/mg protein) from the cytoplasm of a-mating type cells. The purified substance gave a single band stained with PAS reagent but not with both Coomassie brilliant blue and silver staining reagent by polyacrylamide gel electrophoresis in the presence of 8 M urea. However, incorporation of [35S]methionine and Lowry reaction clearly indicate that the substance is a glycoprotein. The substance specifically masked sexual agglutinability of cells of the opposite mating type α, indicating univalent action. The substance is a glycoprotein with a carbohydrate content of 90%, a pI of 4.5, and a molecular weight of 130,000. The substance was inactivated by 2-mercaptoethanol and proteolytic enzymes but not by glycolytic enzymes. The substance formed a complementary complex having no biological activity when mixed with α-agglutination substance from the wall or cytoplasm of α-cells in vitro.
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    Attraction of male spruce budworm moths,Choristoneura fumiferana (Clemens), to pheromone-baited traps in small-tree thinnings (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 125-133 
    Details
    ISSN: 1573-1561
    Keywords: Spruce budworm ; Choristoneura fumiferana ; Lepidoptera ; Tortricidae ; sex pheromone ; small-tree thinnings ; temperature ; precipitation ; wind ; attraction distance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Mean catches of spruce budworm,Choristoneura fumiferana (Clemens), moths were not significantly different among four small-tree thinning treatments of young spruce-fir-hemlock regeneration. Significant inverse relationships were found between trap catches and distances to nearby spruce-fir-hemlock overstory. Prevailing wind directions indicated that moths were attracted anemotactically to upwind pheromone sources. No definite trends were detected between catches and temperature or precipitation.
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    Relative kairomonal activities of 2-acylcyclohexane-1,3-diones in eliciting oviposition behavior from parasiteNemeritis canescens (Grav.) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1597-1601 
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    ISSN: 1573-1561
    Keywords: Kairomone ; 2-acylcyclohexane-1,3-diones ; ovipositionEphestia kuehniella Zeller [syn.Anagasta kuehniella (Zeller)] ; Lepidoptera ; Pyralidae ; Nemeritis canenscens (Grav.) [syn.Venturia canescens (Grav.)] ; Hymenoptera ; Ichneumonidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The relative activities of sixteen 2-acylcyclohexane-1,3-diones from the larval mandibular glands ofEphestia (=Anagasta) kuehniella Zeller in causing the parasiteNemeritis (=Venturia) canescens (Grav.) to make oviposition movements are reported.
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    Potential for evolution of resistance to pheromones: Interindividual and interpopulational variation in chemical communication system of pink bollworm moth (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1551-1565 
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    ISSN: 1573-1561
    Keywords: Resistance ; mating disruption ; sex pheromone ; (Z,Z)-7 ; 11-hexadecadienyl acetate ; (Z,E)-7 ; 11-hexadecadienyl acetate ; Lepidoptera ; Gelechiidae ; pink bollworm ; Pectinophora gossypiella ; cotton ; pheromone collection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract After an extensive examination of the release rates and blend ratios of pheromonal components emitted by field-collected femalePectinophora gossypiella (Saunders), we find no evidence of resistance to pheromones applied to cotton fields to disrupt mating. Females from fields with 3–5 years of exposure to disruptant pheromones as well as those from fields with only minimal exposure to disruptant pheromones emitted (Z,Z)-7,11-hexadecadienyl acetate at a rate of ca. 0.1 ng/min and (Z,E)7,11-hexadecadienyl acetate at ca. 0.06 ng/min. The ratio of pheromonal components was much less variable than the measured emission rate and was centered about a 61:39Z, Z to Z,E ratio. In contrast to the blend ratio emitted by females, the composition of the pheromonal blend used in monitoring populations and disrupting mating is centered about 50:50 Z,Z to Z.E. In general there was a remarkable consistency in the release rate and blend ratio among populations of females throughout southern California and those from a laboratory colony. It would appear that, although resistance to theP. gossypiella pheromone is still a very real possibility when it is used heavily in pest management as a mating disruptant, there are current agricultural practices and conditions which would hinder its development.
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    Structure-activity relationships between stimulus molecule and response of a pheromone receptor cell in turnip moth,Agrotis segetum (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1661-1675 
    Details
    ISSN: 1573-1561
    Keywords: Structure-activity relationships ; single-cell recordings ; turnip moth ; Agrotis segetum ; Lepidoptera ; Noctuidae ; molecular shape ; dipole moments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The response of an antennal receptor cell of the turnip moth,Agrotis segetum, was recorded during stimulation with a series of (Z)-7-dodecenyl acetate analogs with structural variations of the acetate group. The investigated receptor cell is known to be highly selective to (Z)-7-dodecenyl acetate. All parts of the acetate group were found to be of great importance for full biological activity. The results indicate very strict requirements on the shape of the polar functional group, as well as on its electron distribution for a successful interaction with the antennal receptor cell.
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    Plant-determined variation in the cardenolide content, thin-layer chromatography profiles, and emetic potency of monarch butterflies,Danaus plexippus L. Reared on milkweed plants in California: 2.Asclepias speciosa (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 601-639 
    Details
    ISSN: 1573-1561
    Keywords: Danaus plexippus ; Lepidoptera ; Danaidae ; monarch butterflies ; Asdepias speciosa ; Asclepiadaceae ; milkweeds ; ecological chemistry ; plant-insect interactions ; chemical ecology ; chemical defense ; coevolution ; thin-layer chromatography ; cardenolide fingerprints ; cardenolides ; cardiac glycosides ; desglucosyrioside ; labriformin ; labriformidin ; syriogenin ; uzarigenin ; emetic potency ; emesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The pattern of variation in gross cardenolide concentration of 111Asclepias speciosa plants collected in six different areas of California is a positively skewed distribution which ranges from 19 to 344 μg of cardenolide per 0.1 g dry weight with a mean of 90 μg per 0.1 g. Butterflies reared individually on these plants in their native habitats ranged from 41 to 547 μg of cardenolide per 0.1 g dry weight with a mean of 179 μg. Total cardenolide per butterfly ranged from 54 to 1279 μg with a mean of 319 μg. Differences in concentrations and total cardenolide contents in the butterflies from the six geographic areas appeared minor, and there were no differences between the males and the females, although the males did weigh significantly more than females. The uptake of cardenolide by the butterflies was found to be a logarithmic function of the plant concentration. This results in regulation: larvae which feed on low-concentration plants produce butterflies with increased cardenolide concentrations relative to those of the plants, and those which feed on high-concentration plants produce butterflies with decreased concentrations. No evidence was adduced that high concentrations of cardenolides in the plants affected the fitness of the butterflies. The mean emetic potencies of the powdered plant and butterfly material were 5.62 and 5.25 blue jay emetic dose fifty units per milligram of cardenolide and the number of ED50 units per butterfly ranged from 0.28 to 6.7 with a mean of 1.67. Monarchs reared onA. speciosa, on average, are only about one tenth as emetic as those reared onA. eriocarpa. UnlikeA. eriocarpa which is limited to California,A. speciosa ranges from California to the Great Plains and is replaced eastwards byA. syriaca L. These two latter milkweed species appear to have a similar array of chemically identical cardenolides, and therefore both must produce butterflies of relatively low emetic potency to birds, with important ecological implications. About 80% of the lower emetic potency of monarchs reared on A. speciosa compared to those reared onA. eriocarpa appears attributable to the higher polarity of the cardenolides inA. speciosa. Thin-layer Chromatographie separation of the cardenolides in two different solvent systems showed that there are 23 cardenolides in theA. speciosa plants of which 20 are stored by the butterflies. There were no differences in the cardenolide spot patterns due either to geographic origin or the sex of the butterflies. As when reared onA. eriocarpa, the butterflies did not store the plant cardenolides withR f values greater than digitoxigenin. However, metabolic transformation of the cardenolides by the larvae appeared minor in comparison to when they were reared onA. eriocarpa. AlthoughA. eriocarpa andA. speciosa contain similar numbers of cardenolides and both contain desglucosyrioside, the cardenolides ofA. speciosa overall are more polar. ThusA. speciosa has no or only small amounts of the nonpolar labriformin and labriformidin, whereas both occur in high concentrations inA. eriocarpa. A. speciosa plants and butterflies also contain uzarigen, syriogenin, and possibly other polar cardenolides withR f values lower than digitoxin. The cardenolide concentration in the leaves is not only considerably less than inA. eriocarpa, but the latex has little to immeasurable cardenolide, whereas that ofA. eriocarpa has very high concentrations of several cardenolides. Quantitative analysis ofR f values of the cardenolide spots, their intensities, and their probabilities of occurrence in the chloroform-methanol-formamide TLC system produced a cardenolide fingerprint pattern very different from that previously established for monarchs reared onA. eriocarpa. This dispels recently published skepticism about the predictibility of chemical fingerprints based upon ingested secondary plant chemicals.
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    Metabolism of uscharidin, a milkweed cardenolide, by tissue homogenates of monarch butterfly larvae,Danaus plexippus L. (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 945-956 
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    ISSN: 1573-1561
    Keywords: Cardenolide ; uscharidin ; metabolism ; monarch butterfly ; Danaus plexippus ; Lepidoptera ; Danaidae ; milkweed ; Asclepias ; N-demethylation ; mixed function oxidase ; monooxygenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Midgut and fat body homogenates of monarch butterfly larvae,Danaus plexippus L. (Lepidoptera:Danaidae), were examined for microsomal monooxygenase activity usingp-chloro-N-methylanilineN-demethylation and for the ability to metabolize a milkweed (Asclepias spp.) cardenolide (C23 steroid glycoside), uscharidin. All homogenates tested had bothN-demethylation and uscharidin biotransformation activities. Both transformations required NADPH. The monooxygenase inhibitors sesamex, SKF525A, and carbon monoxide inhibitedN-demethylation but not uscharidin biotransformation. Subsequent subcellular fractionation revealed the uscharidin biotransformation occurs in the soluble fraction and not the microsomal fraction, whileN-demethylation occurs in the microsomal fraction and not the soluble fraction. The larval NADPH-dependent microsomal monooxygenase apparently is not involved in the metabolism of uscharidin.
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    Effect of pheromone concentration on organization of preflight behaviors of the male gypsy moth,Lymantria dispar(L.) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 17-23 
    Details
    ISSN: 1573-1561
    Keywords: Gypsy moth ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; preflight male behavior ; (+)-cis-7,8-epoxy-2-methyloctadecane ; (+)-dis-parlure ; wing-fanning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Male gypsy moths (Lymantria dispar L.) in a wind tunnel at 24° respond to decreasing dosages (1 μg to 0.1 pg) of (+)-cis-7,8-epoxy-2-methyloctadecane with a decrease in probability of wing-fanning, an increase in wing-fanning latency, and an increase in the number of behaviors (body jerks, antennal twitches, steps, and wing tremors) preceding fanning. Males initiating any behavior prior to wing-fanning had a 70% probability of wing-fanning and 97% of the males that wing-fanned eventually flew. The sequence of behaviors from quiescence to flight is not organized in a hierarchy, as this concept is used in ethology, nor is it dependent upon the concentration of pheromone. The time-average threshold concentration of pheromone for response of ca. 90% or more quiescent males is ca. 1.9 × 10−17 g/cm3 over 〈 0.3 min.
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    Evaluation of time-average dispersion models for estimating pheromone concentration in a deciduous forest (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1081-1108 
    Details
    ISSN: 1573-1561
    Keywords: Pheromone dispersion ; active space ; Sutton model ; Gaussian plume model ; gypsy moth ; Lymantria dispar ; Lepidoptera ; Lymantriidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The Sutton and more recent Gaussian plume models of atmospheric dispersion were used to estimate downwind concentrations of pheromone in a deciduous forest. Wind measurements from two bivane anemometers were recorded every 12 sec and the pheromone was emitted from a point source 1.6 m above ground level at known rates. The wingfanning response of individually caged male gypsy moths (Lymantria dispar) at 15 sites situated 20 to 80 m downwind was used to monitor when pheromone levels were above threshold over a 15-min interval. Predicted concentrations from these Gaussian-type models at locations where wing fanning occurred were often several orders of magnitude below the known behavioral thresholds determined from wind tunnel tests. Probit analyses of dose-response relationships with these models showed no relationship between predicted dose and actual response. The disparity between the predictions of concentration from these models and the actual response patterns of the male gypsy moth in the field was not unexpected. These time-average models predict concentrations for a fixed position over 3-min or longer intervals, based upon the dispersion coefficients. Thus the models estimate pheromone concentrations for time intervals appreciably longer than required for behavioral response.
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    Identification of new sex pheromone components inTrichoplusia ni, predicted from biosynthetic precursors (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1309-1323 
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    ISSN: 1573-1561
    Keywords: Lepidoptera ; Noctuidae ; Trichoplusia ni ; pheromone ; (Z)-7-dodecenyl acetate ; (Z)-5-dodecenyl acetate ; 11-dodecenyl acetate ; (Z)-7-tetradecenyl acetate ; (Z)-9-tetradecenyl acetate ; biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In addition to the previously identified components (Z)-7-dodecenyl acetate and dodecyl acetate, sex pheromone glands ofTrichoplusia ni release (Z)-5-dodecenyl acetate, 11-dodecenyl acetate, (Z)-7-tetradecenyl acetate, and (Z)-9-tetradecenyl acetate. Bioassays in a flight tunnel showed that a synthetic blend of these six compounds elicited complete flights to the source from 95% of the males tested and elicited hairpenciling responses at the end of the flights from 88% of the males tested. This blend was not significantly different from intact pheromone glands, which elicited complete flights to the source from 98% of the males tested and hairpenciling responses from 91% of the males tested. In contrast, the previously identified two-component blend elicited significantly fewer complete flights to the source (33%) and did not elicit hairpenciling responses from any of the males tested. The search for additional sex pheromone components was prompted by our previous identification of unusual fatty acyl moieties in the gland that seemed to be possible biosynthetic intermediates.
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    Role of pheromone components in evoking behavioral responses from male potato tuberworm moth,Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 271-280 
    Details
    ISSN: 1573-1561
    Keywords: Potato tuberworm moth ; Phthorimaea operculella ; (E,Z)-4,7-tridecadienyl acetate ; (E,Z,Z)-4,7,10-tridecatrienyl acetate ; insect sex pheromone ; Lepidoptera ; Gelechiidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Responses of the male potato tuberworm moth,Phthorimaea operculella (Zeller), to two sex pheromone components and female crude extract were studied. Both in a wind tunnel and the field, males were better able to locate a source consisting of a 1∶1 mixture of (E,Z)-4,7-tridecadien-1-yl acetate and (E,Z,Z)-4,7,10-tridecatrien-1-yl acetate than a source consisting of the triene alone. The addition of the diene increased the time spent in the vicinity of the pheromone source, time spent on the source itself, and also increased the average number of visits to the source per individual when compared to the triene alone. The triene elicited high levels of locomotor activity and may play a major role in eliciting earlier (long-range) steps in the behavioral sequence of sexually activated moths. The diene appeared to influence later (short-range) behavior. The behavioral responses of males to a 1∶1 mixture of the diene and triene were similar to those elicited by female crude extract.
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    Host selection byBlepharipa pratensis (Meigen), a tachinid parasite of the gypsy moth,Lymantria dispar L. (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 311-320 
    Details
    ISSN: 1573-1561
    Keywords: Gypsy moth ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; Blepharipa pratensis ; Diptera ; Tachinidae ; host selection ; contact chemical ; parasite behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The host selection process ofBlepharipa pratensis (Meigen), a tachinid parasite of the gypsy moth,Lymantria dispar L., was investigated. Once in the host's habitat, and following contact with a recently damaged leaf edge (cut, torn, eaten), the fly orients perpendicular to the edge and moves back and forth with the front tarsi grasping the damaged edge. Oviposturing (oviposition intention) may occur. Leaf exudates appear to arrest the fly on the leaf and increase tarsal examination (searching). If an edge of a gypsy moth-eaten leaf is contacted, oviposition usually occurs. Significantly more eggs are laid when host-browsed foliage is encountered, compared to mechanically cut or damaged foliage, indicating response to a cue left by the host during feeding. The number of host-damaged leaf clusters in an area significantly enhances oviposition there; in field-cage tests, significantly more eggs (7911) were laid in simulated-crown areas with all clusters browsed, compared to the adjacent areas containing 1/2 browsed (4200 eggs) and undamaged clusters (2209 eggs). A host selection sequence is suggested and discussed.
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    Sex pheromone of the pea moth,Cydia nigricana (F.) (Lepidoptera: Olethreutidae) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 973-982 
    Details
    ISSN: 1573-1561
    Keywords: Pea moth ; Cydia nigricana (F.) ; Laspeyresia nigricana Steph. ; Lepidoptera ; Olethreutidae ; sex pheromone ; sex attractant ; gas chromatography ; mass spectrometry ; single-ion monitoring ; electroantennography ; (E,E)-8,10-dodecadien-1-yl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The sex pheromone of the pea moth,Cydia nigricana (F.), was identified as (E,E)-8,10-dodecadien-1-ylacetate (E,E8,10–12∶Ac) (approximately 0.1 ng/abdominal tip), in vacuum distillates of virgin female abdominal tips and volatiles emitted by calling females, from its chemical properties and capillary gas chromatography and mass spectrometry data. SyntheticE,E8,10–12∶Ac and the natural pheromone elicited similar quantitative electrophysiological and behavioral responses from male moths. Other related compounds which also attract male moths in the field,viz., (E)-10-dodecen-1-yl acetate, (E,E)-8,10-dodecadien-1-ol, and (E,E)-8,10-dodecadienal, were not detected in the pheromone andE,E8,10–12∶Ac was not found in male moths.
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    Potential uses of kairomones for behavioral manipulation ofCotesia marginiventris (Cresson) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1377-1384 
    Details
    ISSN: 1573-1561
    Keywords: Fall armyworm ; Spodoptera frugiperda ; Lepidoptera ; Noctuidae ; Cotesia ; (=Apanteles)marginiventris ; Hymenoptera ; Ichneumonidae ; kairomone ; parasitism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effects of kairomone pattern and preconditioning on parasitization rates of fall armyworm larvae,Spodoptera frugiperda (J.E. Smith) byCotesia (=Apanteles)marginiventris (Cresson) were investigated in the laboratory and greenhouse using a hexane extract of frass and actual fall armyworm frass. Parasitization rates increased 55 and 26% in Petri dishes and on corn seedlings, respectively, when the entire experimental area was sprayed with the frass extract. Applying the extract in spots resulted in a 20–30% reduction in parasitization compared to treating the entire area. However, spot application produced significantly better parasitization rates compared to the control treatment. Exposing the parasitoids to actual fall armyworm frass resulted in ca. 50% greater retention in the release area and an increase of ca. 60–75% in the number of parasitoids searching.C. marginiventris parasitized factitious hosts topically treated with an extract of fall armyworm frass.
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    Filbertworm sex pheromone (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 53-61 
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    ISSN: 1573-1561
    Keywords: Melissopus latiferreanus ; Lepidoptera ; Tortricidae ; Olethreutinae ; filbertworm ; sex pheromone ; sex attractant ; (E,E)-8 ; 10-dodecadien-1-ol acetate ; (E,Z)-8,10-dodecadien-1-ol acetate ; conjugated diene isomerization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (E,E)- and (E,Z)-8,10-Dodecadien-1-ol acetates were identified in a 1∶4.3 ratio in the extract of abdominal tips of female filbert-worm moths,Melissopus latiferreanus (Walsingham). The identifications were based on electroantennogram (EAG) analysis, gas chromatography, mass spectrometry, ozonolysis, and synthesis. TheE,Z isomer produced the stronger EAG response. In the field tests of various ratios ofE,E∶E,Z, the ratio found in the extract captured the most males. The pureE,E isomer initially was not attractive by itself (〈0.1%E,Z) but became attractive after a few days, presumably because of isomerization. TheE, Z isomer (〈0.1%E,E) was attractive initially, but this compound might have isomerized faster than theE,E isomer. A study of the isomerization showed that regardless of the initial mixture of 8,10-dodecadien-1-ol acetate isomers, almost complete equilibration existed after one month. The equilibrium mixture consisted of 9%Z8,E10, 65%E8,E10, 23%E8,Z10, and 3%Z8,Z10. Concentrations in rubber septa (1∶4 ratio ofE,E toE,Z) of 0.03–3.0 mg/septum produced equivalent trap catches.
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    New sex attractants for 35 tortricid and 4 other lepidopterous species, found by systematic field screening in the Netherlands (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 135-144 
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    ISSN: 1573-1561
    Keywords: Sex attractants ; field screening ; Lepidoptera ; Tortricidae ; Olethreutinae ; dodecen-1-ol ; dodecen-1-ol acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Most of the known sex attractants and pheromones found for Tortricidae attract species of the subfamily Tortricinae. In order to find more sex attractants for species of the subfamily Olethreutinae, about 60 one- and two-component lures were screened for attractancy in different biotopes in the Netherlands. Most of the chemicals tested were straight-chain dodecen-1-ols and their acetates. The species captured belonged to the following families and subfamilies: Tortricinae (5), Olethreutinae (30), Noctuidae (2), Gelechiidae (1), Gracillariidae (1). Some of the species captured are recorded as pest species in forestry:Epinotia tedella, Gypsonoma aceriana, Cydia strobilella, C. zebeana, Petrova resinella, Blastesthia turionella, andB. posticana.
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    Sex attractant for currant clearwing mothSynanthedon tipuliformis (Clerck) (Lepidoptera: Sesiidae) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1371-1376 
    Details
    ISSN: 1573-1561
    Keywords: Attractant ; Synanthedon tipuliformis ; Lepidoptera ; Sesiidae ; (E,Z)-2,13-octadecadien-1 -ol acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The currant clearwing moth,Synanthedon tipuliformis (Clerck) (Lepidoptera: Sesiidae), is a pest in many parts of the world. In field tests it was found that (E,Z)-2,13-octadecadien-1-ol acetate attracts males of this species. The synthesis of this compound and of its geometrical isomer (Z,Z)-2,13-octadecadien-1-ol acetate is described.
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    Novel sex pheromone components from the fall cankerworm moth,Alsophila pometaria (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 463-473 
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    ISSN: 1573-1561
    Keywords: Alsophila pometaria (Harris) ; fall cankerworm ; Lepidoptera ; Geometridae ; sex pheromone ; (Z,Z,Z)-3,6,9-nonadecatriene ; (Z,Z,Z,E)-3,6,9,11-nonadecatetraene ; (Z,Z,Z,Z)-3,6,9,11-nonadecatetraene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A sex pheromone extract from fall cankerworm moths,Alsophila pometaria, attracted conspecific males in field tests. Four EAG-active components were isolated from the extract and identified by GC-MS, highfield PMR spectroscopy, and microchemical techniques asn-nonadecane (I), (Z,Z,Z)-3,6,9-nonadecatriene (II), (Z,Z,Z,E)-3,6,9,11-nonadecatetraene (III), and (Z,Z,Z,Z)-3,6,9,11-nonadecatetrane (IV). Studies of the behavioral responses of male moths in a flight tunnel to the isolated components showed II, III, and IV were the major components of the sex pheromone. No sex pheromone behavioral responses were observed for I.
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    Role of (Z)- and (E)-11-tetradecenyl acetate pheromone components in the sexual behavior of the (Z) strain of the european corn borer,Ostrinia nubilalis (Lepidoptera: Pyralidae) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 9-15 
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    ISSN: 1573-1561
    Keywords: Ostrinia nubilalis ; Lepidoptera ; Pyralidae ; European corn ; borer ; pheromone ; (Z)-11-tetradecenyl acetate ; (E)-11-tetradecenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A glass tube olfactometer bioassay was used to examine pheromone response of males of the (Z)-pheromone strain ofOstrinia nubilalis (Hubner). The presence of (E)-11-tetradecenyl acetate at the natural ratio to (Z)-11-tetradecenyl acetate (97∶3; Z∶E) did not consistently elevate wing-fanning, upwind walking, or clasper extrusion over (Z)-11-tetradecenyl acetate alone. This bioassay did not reveal the behavioral role of (E)-11-tetradecenyl acetate.
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    Mate location strategies of gypsy moths in dense populations (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 25-31 
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    ISSN: 1573-1561
    Keywords: Lymantria dispar ; Lepidoptera ; Lymantriidae ; gypsy moth ; attractant ; pheromone ; population densities ; sexual selection ; location strategy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In high-density populations, the male gypsy moth (Lymantria dispar) “appetitive” flight is primarily vertical and within 1–50 cm of tree boles. Preceding location of a female, males land on trees or occasionally tree foliage and walk while wing-fanning. In high-density populations females may mate before calling, often prior to wing expansion or hardening. Additional matings may occur before any or full deposition of egg masses. Virgin females are not coy, whether their wings are unexpanded, or hardened and held rooflike, or whether they are calling or not. They generally mate with the first willing male and do not exercise sexual selection by an acceptance or rejection reaction. The mating structure in dense populations would seem to reduce selective pressure for female emission of and direct male anemotactic flight to attractant pheromone. A low proportion of males “search” appropriate objects, especially tree trunks, in the walking-wing-fanning state. Such males often are successful in locating virgin females before calling commences. This male strategy presumably would not be successful in low population densities.
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    Plant-determined variation in cardenolide content and thin-layer chromatography profiles of monarch butterflies,Danaus plexippus reared on milkweed plants in California (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1823-1857 
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    ISSN: 1573-1561
    Keywords: Danaus plexippus ; Lepidoptera ; Danaidae ; monarch butterflies ; Asclepias californica ; Asclepiadaceae ; milkweeds ; ecological chemistry ; plant-insect interactions ; chemical ecology ; chemical defense ; chemotaxonomy ; coevolution ; thin-layer chromatography ; cardenolide fingerprints ; cardenolides ; calotropagenin glycosides ; calactin ; calotropin ; uscharidin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Variation in gross cardenolide concentration of the mature leaves of 85Asclepias californica plants collected in four different areas of California is a positively skewed distribution ranging from 9 to 199 μg of cardenolide per 0.1 g dry weight with a mean of 66 μg/0.1 g. Butterflies reared individually on these plants in their native habitats contained a normal distribution of cardenolide ranging from 59 to 410 μg of cardenolide per 0.1 g dry weight with a mean of 234 μg. Cardenolide uptake by the butterflies was a logarithmic function of plant concentration. Total cardenolide per butterfly ranged from 143 to 823 μg with a mean of 441 μg and also was normally distributed. Populational variation of plant cardenolide concentrations occurs within subspecies, but the northern subspeciesA. c. greenei does not differ significantly from the southernA. c. californica. Generally higher concentrations occur in butterflies from northern populations and in females. No evidence was adduced that cardenolides in the plants adversely affected the butterflies. Low cardenolide concentrations in the leaves and the absence of cardenolides in the latex characterize bothA. californica andA. speciosa, but notA. eriocarpa. Thin-layer chromatography in two solvent systems isolated 24 cardenolide spots in the plants, of which 18 are stored by the butterflies. There was a minor difference in the cardenolide spot patterns due to geographic origin of the plants, but as in our previous studies, none in the sexes of the butterflies. UnlikeA. eriocarpa andA. speciosa, A. californica plants lack cardenolides withRf values greater than digitoxigenin. Overall, the cardenolides of bothA. californica andA. speciosa are more polar than those inA. eriocarpa. A. californica plants contain cardenolides of the calotropagenin series including calotropin, calactin, and uscharidin, and the latter is metabolically transformed by monarch larvae to calactin and calotropin. Cardenolides of this series also occur inA. vestita, andA. cordifolia from California, the neotropicalA. curassavica, and the AfricanCalotropis procera, Gomphocarpus spp., andPergularia extenso; they therefore cross established taxonomic lines.A. californica is the predominant early season milkweed in California and may be important in providing chemical protection to the spring generation of monarchs in the western United States.A. speciosa, A. eriocarpa, andA. californica each imparts distinctive cardenolide fingerprints to the butterflies, so that ecological predictions are amenable to testing.
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    Effects of dodecyl acetate andZ-10-tridecenyl acetate on attraction ofEupoecilia ambiguella males to the main sex pheromone component,Z-9-Dodecenyl acetate (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 253-264 
    Details
    ISSN: 1573-1561
    Keywords: Z-9-Dodecenyl acetate ; Z-10-tridecenyl acetate ; dodecyl acetate ; sex pheromone ; synergism ; behavior ; wind tunnel ; field trapping ; European grape moth ; Eupoecilia ambiguella ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Attraction ofE. ambiguella males toZ-9-dodecenyl acetate (Z9-12∶Ac), alone and in combination with dodecyl acetate (12∶Ac) orZ-10-tridecenyl acetate (Z10-13∶Ac) was tested in both wind-tunnel and field experiments. In the laboratory, response toZ9-12∶Ac reached a distinct dosage optimum at which attraction was nearly as good as to live females. Addition of 12∶Ac, a minor component of female glands and effluvia, had no effect at low doses ofZ9-12∶Ac and only marginally improved attraction at the optimum. However, inclusion of 12∶Ac with an overdose ofZ9-12∶ Ac fully restored activity. In the field,Z9-12∶Ac alone was not very attractive at any dose; catches markedly increased by adding 12∶Ac and climbed with increasing dose of each compound up to 10 mg. Optimum ratio ofZ9-12∶Ac to 12∶Ac was 1∶1 to 1∶5. Another compound,Z10-13∶Ac, had the same effect as 12∶Ac in the laboratory and the field but was effective at a lower dose. In the field, inclusion of the synergist permits use of high cap loads to attract more males, perhaps from greater distances.
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    Trimerization ofEarias insulana sex pheromone, (E,E)-10,12-Hexadecadienal, A phenomenon affecting trapping efficiency (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 421-428 
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    ISSN: 1573-1561
    Keywords: Earias insulana ; spiny bollworm ; Lepidoptera ; Noctuidae ; sex pheromone ; (E,E)-10,12-hexadecadienal ; trimerization ; Chromatographic analysis ; nuclear magnetic resonance ; mass spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The sex pheromone ofEarias insulana, (E,E)-10,12-hexadecadienal, may trimerize extensively to form a crystalline trioxane derivative. The structure of the trimer was deduced from its CI-MS and NMR spectra. Capillary GC analysis resulted in the thermal decomposition of the trimer to the monomer. This process could be studied on a 2-m packed column under specific conditions. A convenient separation between the pheromone and its trimer was achieved by TLC. The trimer was inactive in the field, and it has a harmful effect on the performance of the polyethylene dispenser. Material which contains large amounts of the trimer is unsuitable for field use, even if applied at high dosage. The pheromone should be analyzed by NMR or TLC in addition to GC in order to detect the presence of its trimer. The trimerization process is catalyzed by acid which should therefore be completely eliminated from the storing vessels.
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    Eucalyptus oils in larvae of gum emperor moth,Antheraea eucalypti (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1489-1496 
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    ISSN: 1573-1561
    Keywords: Antheraea eucalypti ; Saturniidae ; Lepidoptera ; larval oral ; discharge ; Eucalyptus oils ; monoterpenoids ; sesquiterpenoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The ether-soluble portion of the foregut fluid from the larvae ofAntheraea eucalypti (Saturniidae) was found to contain traces (0.1–0.2%) of isoprenoids. The isoprenoids were those that were major components of the oils from the leaves of theEucalyptus species on which the larvae fed and were also soluble in the foregut fluid. This observation demonstrated that these larvae did not sequester the leaf oils in their foregut or use them for defense. The compositions of the oils, which were steam-distilled from the bodies and feces of the larvae, were identical with those of the oils from the leaves of the host trees. This fact implied that the volatile components of the leaf oils had not been metabolized and had no effect on the growth or feeding of the larvae, at least on the twoEucalyptus species examined. In order to explain the coevolution ofA. eucalypti andEucalyptus species, components of the leaf oils were suggested to serve the function of an ovipositional attractant to the female moths.
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    Iridoid glycosides and host-plant specificity in larvae of the buckeye butterfly,Junonia coenia (Nymphalidae) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1567-1577 
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    ISSN: 1573-1561
    Keywords: Iridoid glycoside ; Junonia coenia ; Lepidoptera ; Nymphalidae ; host-plant specificity ; coevolution ; insect-plant interaction ; Plantago lanceolata ; buckeye ; chemical ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Larvae of the buckeye,Junonia coenia (Nymphalidae) feed primarily on plants in four families: Scrophulariaceae, Plantaginaceae, Verbenaceae, and Acanthaceae. These plant families have in common the presence of a group of plant secondary compounds, the iridoid glycosides. Larvae were reared on three plant species and two artificial diets, one with and one without iridoid glycosides.Larvae grew poorly and had low survivorship on the artificial diet without iridoid glycosides, while growth and survival on the artificial diet with iridoid glycosides was comparable to that on plants. Choice tests using artificial diets with and without iridoid glycosides showed that larvae: (1) chose diets with iridoid glycosides (in the form of a crude extract or pure compound) over a diet without; (2) showed no preference between the diet with the crude extract and that with pure iridoid glycoside, and (3) preferred the artificial diet with ground leaves of the host plant,Plantago lanceolata, over the diet with pure iridoid glycosides. The artificial diet that larvae had been reared on prior to these tests had no effect on subsequent larval preferences in the choice tests.
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    Redundancy in a chemical signal: Behavioral responses of maleTrichoplusia ni to a 6-Component sex pheromone blend (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1635-1658 
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    ISSN: 1573-1561
    Keywords: Trichoplusia ni ; cabbage looper moth ; Lepidoptera ; Noctuidae ; pheromone ; redundancy ; flight tunnel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The flight response of maleTrichoplusia ni was observed in a flight tunnel to a sex pheromone blend composed of six components:Z7–12∶Ac, 12∶Ac,Z5-12∶Ac, 11-12∶Ac,Z7-14∶Ac, and Z9-14∶Ac. The number of males reaching a 3000-μg source of this blend was 〉 95%, equal to that observed to female glands and significantly greater than with the previously identified two-component blend (Z7-12∶Ac + 12∶Ac). In subtraction tests, all five-component blends, with the exception of the blend lacking the primary componentZ7-12∶Ac, and several four-component blends elicited similar peak levels of upwind flight, source contacts, and hairpencil displays to that observed with the six-component blend. We characterize the substitution of certain minor components for one another as a form of redundancy in the chemical signal and suggest that it contributes to response specificity and signal recognition in males. The results also support the concept that the full blend of components acts as a unit to influence male behavior at all phases of the response. Individual minor components were not responsible for eliciting specific behaviors in the sequence.
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    Sex attractant for three species of the genusOncocnemis: O. chandleri (Grt.),O. cibalis (Grt.), andO. mackiei (B. & Benj.) (Lepidoptera: Noctuidae) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1701-1706 
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    ISSN: 1573-1561
    Keywords: Lepidoptera ; Noctuidae ; Oncocnemis chandleri ; Oncocnemis cibalis ; Oncocnemis mackiei ; (5E7Z)-5 ; 7-dodecadienyl acetate ; (Z)-7-dodecenyl acetate ; sex attractant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Oncocnemis chandleri, O. cibalis, andO. mackiei were attracted to chemically baited traps in the field. In all three cases, (5E,7Z)-dodecadienyl acetate was a key component for attraction. Attraction ofO. chandleri to traps baited with the (5E,7Z)-dodecadienyl acetate was inhibited by addition of (Z)-7-dodecenyl acetate.O. cibalis required both (5E,7Z)-dodecadienyl acetate and (Z)-7-dodecenyl acetate for attraction. Electroantennogram responses for the three species are also reported.
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    Male wing-gland pheromone ofEphestia elutella (1984)
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    Journal of chemical ecology 10 (1984), S. 667-679 
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    ISSN: 1573-1561
    Keywords: Ephestia elutella ; Lepidoptera ; Pyralidae ; male pheromone ; pheromone bioassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Sex pheromone extracted from glands on the forewings of maleEphestia elutella (Hübner) elicits a stereotyped courtship response from conspecific females. A bioassay for this sex pheromone was developed based on this behavior. Maximum production and responsiveness for males and females, respectively, occurred in insects more than 24 hr old.E. elutella females were not responsive to extracts made fromE.figulilella Gregson,E. kuehniella Zeller,E. cautella (Walker), orPlodia interpunctella (Hübner) males.
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    Biosynthesis of sex pheromone components and glycerolipid precursors from sodium [1-14C]acetate in redbanded leafroller moth (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 681-691 
    Details
    ISSN: 1573-1561
    Keywords: Sex pheromone biosynthesis ; Argyrotaenia velutinana ; redbanded leafroller moth ; Lepidoptera ; Tortricidae ; radiolabel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Sodium [1-14C]acetate in water-dimethyl sulfoxide (1∶1) was applied topically to sex pheromone glands ofArgyrolaenia velutinana. Radiolabel was incorporated into the pheromone components (Z)-11-tetradecenyl acetate and (E)-11-tetradecenyl acetate, and also into triacylglycerols, diacylglycerols, ethanolamine phosphatides, and choline phosphatides. In the triacylglycerols, radiolabel appeared in (Z)-11-tetradecenoate, (E)-11-tetradecenoate, tetradecanoate, hexadecanoate, and octadecanoate. In the choline phosphatides, the same acyl moieties incorporated radiolabel but at lower levels. In the diacylglycerols and ethanolamine phosphatides, only the radiolabel in hexadecanoate and octadecanoate was above the limit of detection. At different times following application of sodium [1-14C]acetate, the relative proportions of labeled (Z)-11-tetradecenyl acetate and (E)-11-tetradecenyl acetate changed very little, but the relative proportions of labeled fatty acyl moieties in the triacylglycerols and choline phosphatides changed markedly. After 8 min, triacylglycerols had incorporated about equal amounts of radiolabel into (Z)-11-tetradecenoate, (E)-11-tetradecenoate, and tetradecanoate. As the incubation time was increased, triacylglycerols accumulated proportionately more radiolabeled (E)-11-tetradecenoate than (Z)-11-tetradecenoate, and accumulated proportionately less radiolabeled tetradecanoate. In the choline phosphatides, at all times of incubation the amount of radiolabel incorporated into (Z)-11-tetradecenoate was small but above the limit of detection, and the amounts of radiolabel in (E)-11-tetradecenoate and tetradecanoate were smaller and often below the limit of detection. In both the triacylglycerols and the choline phosphatides, the relative proportion of radiolabeled hexadecanoate decreased with time, and that of octadecanoate increased.
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    Sex pheromone of a conifer-feeding budworm,Choristoneura retiniana, Walsingham (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 153-160 
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    ISSN: 1573-1561
    Keywords: Choristoneura retiniana ; Lepidoptera ; Tortricidae ; sex pheromone ; sex attractant ; forset insects ; Tortricidae ; insect behavior ; E- andZ-11-tetradecenyl acetates ; E- andZ-11-tetradecenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Sex pheromone components collected from femaleChristoneura retiniana included 11-tetradecenyl acetates and alcohols. The major component wasE-11-tetradecenyl acetate (E11–14∶Ac) with a lesser amount ofZ isomer necessary to induce male response. A 92∶8 ratio ofE,Z11–14∶ Ac appeared optimal. The alcohol component was present at about 10% of the total pheromone mixture, and traps baited with acetates plus alcohol surpassed unmated females in their degree of attractiveness. Chemical analysis indicated a 9∶:1 ratio of theE — Z isomers of 11-tetradecenyl alcohol (11–14∶ol) pheromone components, although bait formulations containing a predominance of either theE orZ isomers were equally successful in field bioassays. Based on male response to traps,E- andZ11–14∶Ac (92∶8E — Z) are essential pheromone components for long-range sex attraction. The 11–14∶ ol enhanced attraction when added at 10% of the total pheromone blend.
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    Role of diet in host selection ofHeliothis virescens by parasitoidCampoletis sonorensis (Hymenoptera: Ichneumonidae) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1535-1541 
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    ISSN: 1573-1561
    Keywords: Campoletis sonorensis ; Heliothis virescence ; synomone ; kairomone ; host location ; Hymenoptera ; Ichneumonidae ; Lepidoptera ; Noctuidae ; parasitoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Gas chromatographic analysis revealed that chemicals attractive toCampoletis sonorensis (Cameron), previously identified in cotton, were not present in wheat germ diet-rearedHeliothis virescens (F.) larvae. Diet-reared larvae fed cotton obtained the chemicals from cotton, with the consequence of enhanced kairomonal activity of the larvae and their frass toC. sonorensis. Parasitoids, presented a choice between cotton, cotton plus hosts, hosts alone, and control in an olfactometer, responded non-randomly, with the greatest number of responses to cotton plus hosts, and three times as many responses to cotton alone as to larvae alone. The role of the plant in the parasitoid-host relationship is discussed.
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    Sex pheromone components of fall cankerworm moth,Alsophila pometaria (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1579-1596 
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    ISSN: 1573-1561
    Keywords: Alsophila pometaria(Harris) ; fall cankerworm ; Lepidoptera ; Geometridae ; sex pheromone ; (Z,Z,Z,E)-3,6,9,11-nonadecatetraene ; (Z,Z,Z,Z)-3,6,9,11-nonadecatetraene ; (Z,Z,Z)-3,6,9-nonadecatriene ; synthesis ; hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (Z,Z,Z,E)-3,6,9,11-Nonadecatetraene and (Z,Z,Z,Z)-3,6,9,11-nonadecatetraene, sex pheromone components ofAlsophila pometaria, were synthesized by stereoselective Wittig reactions and found to be spectroscopically and chromatographically identical to isolated natural material. Flight-tunnel bioassays and field-trapping experiments confirmed that the two tetraenes together with (Z,Z,Z)-3,6,9-nonadecatriene are sex pheromone components. While traps baited with either tetraene individually captured conspecific males in field-trapping experiments, addition of the triene, which captured no males by itself, to either tetraene resulted in synergistic responses.
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    Syntheses of unconjugated (Z,Z)-diolefinic insect pheromones on insoluble polymer supports (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 321-333 
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    ISSN: 1573-1561
    Keywords: (Z, Z)-3,13-Octadecadien-1-yl acetate ; (Z, Z)-7,11-hexadecadien-1-yl acetate ; solid phase synthesis ; Synanthedon pictipes ; lesser peachtree borer ; Synanthedon exitiosa ; peachtree borer ; Synanthedon hector ; cherry tree borer ; Pectinophora gossypiella ; pink bollworm moth ; Lepidoptera ; Sessiidae ; Gelechiidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A 2% cross-linked styrene-divinylbenzene copolymer containing pendant trityl chloride groups was used as the solid support in the synthesis of (Z, Z)-3,13-octadecadien-1-yl acetate, a component of the sex attractant of the lesser peachtree borer,Synanthedon pictipes, the peachtree borer,Synanthedon exitiosa, and the cherry tree borer,Synanthedon hector. This solid-phase synthesis is compared with a similar synthetic approach in solution. The solid-phase synthesis of (Z, Z)-7,11-hexadecadien-1-yl acetate, a component of the pheromone of the pink bollworm moth,Pectinophora gossypiella is described.
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    Behavioral and biological responses ofCotesia marginiventris to kairomones of the fall armyworm,Spodoptera frugiperda (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 521-529 
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    ISSN: 1573-1561
    Keywords: Cotesia marginiventris ; Hymenoptera ; Braconidae ; Spodoptera frugiperda ; Lepidoptera ; Noctuidae ; kairomones ; fall armyworm ; parasitism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Potential kairomone sources of the fall armyworm (FAW),Spodoptera frugiperda (J.E. Smith), were bioassayed with females ofCotesia marginiventris (Cresson) in Petri dishes (10 cm diam). MatedC. marginiventris females, ranging in age from 1 to 3 days exhibited the most intense bioassay responses to potential sources of kairomone. Contacting a FAW-damaged corn leaf modified the pattern of movement inC. marginiventris from random to one exhibiting a significant increase in klinokinesis. No significant differences were present in kairomone responses of nonconditioned and conditioned parasitoids and parasitoid response to kairomones did not change throughout the photophase (0800–1800 hr). Removal of one, both, or the first eight antennal segments reduced or eliminated the response of the parasitoid to kairomones. Female parasitoids did not exhibit a preference for corn leaves damaged by a particular fall armyworm instar and parasitization rates were highest in larvae 48 hr old.
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    Variability in accumulation of proanthocyanidins (condensed tannins) in needles of Douglas-fir (Pseudotsuga menziesii) following long-term budworm defoliation (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1469-1476 
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    ISSN: 1573-1561
    Keywords: Proanthocyanidins ; condensed tannins ; plant-herbivore interactions ; Douglas-fir ; Pseudotsuga menziesii ; western spruce budworm ; Choristoneura occidentalis ; Lepidoptera ; Tortricidae ; nested ANOVA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Long-term defoliation by budworms was associated with higher levels of soluble proanthocyanidins in the current year needles of Douglas-fir trees. The proanthocyanidin contents of needles from defoliated Douglas-fir trees were considerably more variable than those levels of undefoliated ones. The increased mean and variability of proanthocyanidin levels following defoliation may have interesting ecological consequences for Douglas-fir and its defoliators.
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    Fate of photosensitizing furanocoumarins in tolerant and sensitive insects (1984)
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    Journal of chemical ecology 10 (1984), S. 893-911 
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    ISSN: 1573-1561
    Keywords: Xanthotoxin ; psoralen ; isopsoralen ; furanocoumarin ; Papilio polyxenes ; Lepidoptera ; Papilionidae ; Spodoptera frugiperda ; Noctuidae ; metabolism ; detoxification ; tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The fate of [14C]xanthotoxin (8-methoxypsoralen) was studied in larvae of insect species that are tolerant (Papilio polyxenes Stoll) or sensitive (Spodoptera frugiperda J.E. Smith) to the phototoxic effects of photosensitizing psoralens. Both insects metabolize xanthotoxin by oxidative cleavage of the furan ring, but the detoxification occurs at a much more rapid rate inP. polyxenes in which 〉95% of an oral 5 μg/g xanthotoxin dose is metabolized within 1.5 hr after treatment. The detoxification of psoralens byP. polyxenes appears to occur primarily in the midgut tissue prior to absorption, with the result that the intact phototoxin does not reach appreciable levels in body tissues. Studies with an angular furanocoumarin indicated that isopsoralens are metabolized byP. polyxenes at a somewhat slower rate than observed for psoralens; however, a reduced rate of metabolic detoxification of isopsoralens probably does not explain the fact that psoralen tolerance inP. polyxenes does not extend to the isopsoralen series.
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    Sources of fall armyworm,Spodoptera frugiperda (Lepidoptera: Noctuidae), kairomones eliciting host-finding behavior inCotesia (=Apanteles) marginivenitris (Hymenoptera: Braconidae) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1019-1027 
    Details
    ISSN: 1573-1561
    Keywords: Fall armyworm ; Spodoptera frugiperda ; Lepidoptera ; Noctuidae ; kairomone ; host-finding behavior ; Cotsia (=Apanteles)marginiventris ; Hymenoptera ; Braconidae ; frass attraction ; scales attraction ; pest management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Bioassay responses inCotesia marginiventris (Cresson) females to materials derived from fall armyworm (FAW) larvae,Spodoptera frugiperda (J. E. Smith), were most intense for frass and somewhat less intense for larval and pupal cutical materials, scales, exuviae, silk, and oral secretion, with FAW larval hemolymph eliciting only a slight response. The highest percentage of ovipositor probing was caused by frass (100%) and moth scales (90%). Various types of corn-leaf damage when assayed alone did not produce responses as intense as when assayed in combination with frass, cuticle material, and oral secretion. Parasitoid response was somewhat better to frass derived from FAW larvae feeding on corn and peanut leaves than from larvae feeding on the foliage of soybeans, Bermuda grass, cowpeas, or laboratory diet. Hexane and chloroform were better than methanol and water for extracting active material from FAW frass, and chloroform was the best of these solvents for extracting corn leaves. Serial dilutions of frass extracts resulted in a reduction in parasitoid response.
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    Behavioral responses of maleHeliothis zea moths in sustained-flight tunnel to combinations of 4 compounds identified from female sex pheromone gland (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 193-202 
    Details
    ISSN: 1573-1561
    Keywords: Corn earworm ; Heliothis zea ; Lepidoptera ; Noctuidae ; flight tunnel ; sex pheromone ; moth behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Each of the four compounds that have been identified from sex pheromone glands ofHeliothis zea female moths was examined for its ability to elicit sexual responses from male moths in a flight tunnel. Males flew upwind to (Z)-11-hexadecenal alone, but greater levels of behavioral activity were evoked with the addition of (Z)-9-hexadecenal to the treatment. Addition of hexadecanal or (Z)-7-hexadecenal to the initial two components had no effect in raising the behavioral response of the males in the flight tunnel whether added singularly at both the normal gland-emission ratio or at varying ratios or in combination at the normal ratio. Live, calling females elicited levels of sexual activity from males not significantly different from that elicited by the mixture of (Z)-11- and (Z)-9-hexadecenal on cotton wicks.
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    Multichemical resistance of the coniferPodocarpus gracilior (Podocarpaceae) to insect attack (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 547-559 
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    ISSN: 1573-1561
    Keywords: Podocarpus gracilior ; conifer ; resistance ; multichemical defense ; norditerpene dilactones ; phytoecdysone ; biflavones ; Pectinophora gossypiella ; Heliothis zea ; Spodoptera frugiperda ; Lepidoptera ; feeding deterrency ; Bombyx mori
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Podocarpus gracilior is resistant in nature to insect attack. Apparently, the resistance ofP. gracilior is due to a multichemical defense mechanism. Chemicals identified as potential components of the multichemical defense are four norditerpenedilactones, including nagilactones, C, D, and F, which cause insect feeding deterrent activity ultimately coupled to an insecticidal activity, and podolide, an insecticide; two nonlethal growth-inhibiting biflavones, podocarpusflavone A and 7″,4′″-dimethylamentoflavone; and the ecdysis-inhibiting phytoecdysone, ponasterone A.
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    Effect of foliage proximity on attraction ofChoristoneura occidentalis andC. retiniana (Lepidoptera: Tortricidae) to pheromone sources (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 217-227 
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    ISSN: 1573-1561
    Keywords: Pheromonetrap placement ; western spruce budworm ; modoc budworm ; Choristoneura occidentalis ; Choristoneura retiniana ; Lepidoptera ; Tortricidae ; sex pheromone ; sex attractant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Pheromone-baited traps located close to both host and nonhost crowns were more attractive than traps located between crowns for bothC. occidentalis Freeman andC. retiniana (Walsingham) at both 10 m and at 1.5 m above the ground. At 10 m height, traps located in host foliage were more attractive than those located in nonhost foliage, but at 1.5m height there was no significant difference. These results were obtained for both dense and sparse populations ofC. occidentalis and sparse populations ofC. retiniana. We conclude that the tree species on which a virgin female is located is not an important factor restricting mating between closely relatedChoristoneura spp. Also, the tree species on which a trap is located may not be an important factor that must be standardized in developing pheromone monitoring systems forC. occidentalis andC. retiniana.
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    Behavioral effects of secondary components of sex pheromone of western spruce budworm (Choristoneura occidentalis) free (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 265-270 
    Details
    ISSN: 1573-1561
    Keywords: Choristoneura occidentalis ; western spruce budworm ; Lepidoptera ; Tortricidae ; pheromones ; behavior ; wind tunnel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In a laboratory wind tunnel, upwind flight and close-range orientation to a pheromone source by maleC. occidentalis were facilitated by the addition of the secondary componentsE/Z11-tetradecen-1-ol acetate (89∶11) andE/Z11-tetradecen-1-ol (85∶15) to low source concentrations of the primary components,E/Z11-tetradecenal (92∶8). Male responses to the blends tested never equalled their responses to virgin females. The primary components alone, when released at a rate similar to that of a “calling” female, never elicited male upwind flight or source location. However, the addition of the secondary components enhanced these behavioral sequences.
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    Apparency of pulsed and continuous pheromone to male gypsy moths (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 335-348 
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    ISSN: 1573-1561
    Keywords: Pheromone ; attractant ; gypsy moth ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; threshold ; anemotaxis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Quiescent male gypsy moths (Lymantria dispar) exposed in a wind tunnel to either pulsed (0.5-, 2- or 5-sec on, followed by a two-fold time interval off) or continuous streams of synthetic pheromone responded similarly in the proportions and latencies of wing fanning. Similarly, upwind anemotactic flight tracks in pulsed (1-sec on and 1-sec off) and continuous plumes of pheromone were indistinguishable. These data suggest that in the gypsy moth (1) pulsed pheromone stimuli would not lower the threshold, despite the improvement in the signal-to-noise ratio; and (2) temporal modulation of the pheromone plume at 1-sec intervals does not alter the “preprogrammed” upwind flight pattern.
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    Suitability of selected leguminous plants for development of Maruca testulalis larvae (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 174-178 
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    ISSN: 1570-7458
    Keywords: Lepidoptera ; Pyralidae ; Maruca testulalis ; Pod borer ; Development ; Nutritional suitability ; Host plant ; Crotalaria ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les possibilités de développement larvaire de Maruca testulalis sur les fleurs de huit espèces végétales ont été examinées en laboratoire. La comparaison a porté sur Crotalaria retusa, C. juncea, C. saltiana, C. misereniensis, C. mucronata, C. amazonas, Cajanus cajan et l'hôte principal de cette chenille, Vigna unguiculata. En tenant compte de la mortalité larvaire et des indices de croissance (G.I.), ces plantes ont été divisées en trois catégories principales: 1 celles provoquant une mortalité larvaire de 0–30% et ayant des G.I. ≥60%, constituent les plantes hôtes convenables (Vigna unguiculata seule); 2 les plantes qui provoquent une mortalité larvaire de 30≤50% et ont des G.I. de 30 à 60% de la plante hôte principale (Cajanus cajan, Crotalaria amazonas, C. saltiana, C. mucronata; 3 les plantes qui causent 50–100% de mortalité larvaire et dont les G.I. sont inférieurs à 30% de la plante hôte principale (Crotalaria retusa, C. juncea, C. misereniensis). Les résultats déjà publiés sur les choix du lieu de ponte des femelles et l'utilisation de C. juncea comme plante piège, sont discutés à la lumière de ces données nouvelles.
    Notes: Abstract Flowers of eight plant species were evaluated under laboratory conditions for their suitability as larval growth media for the cowpea pod borer, Maruca testulalis. The plants tested were Crotalaria retusa, C. juncea, C. saltiana, C. misereniensis, C. amazonas, Cajanus cajan and the principal host of the borer, Vigna unguiculata (cowpea), was included for comparison. Based on the data obtained on larval mortality and growth indices (GI) the plants were divided into 3 categories namely: I: Those causing 0–30% mortality and having GI value ≥60% form suitable host plants. This group only included V. unguiculata. — II: Those plant species causing 30≤50% larval mortality and having GI value 30%≤60% of the principal host plant (Cajanus cajan, Crotalaria amazonas, C. saltiana, C. mucronata). This group of species is marginally suitable as hosts. — III: Plants causing 50–100% larval mortality and having GI value ≤30% of principal host plant (C. retusa, C. juncea and C. misereniensis). Previously published data on the oviposition preference of the adult moth are discussed in the light of the present findings and the use of C. juncea as a possible trap crop.
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    Sex attractants for clearwing moths: Synanthedon vespiformis and Chamaesphecia tenthrediniformis (and/or C. empiformis) (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 203-205 
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    ISSN: 1570-7458
    Keywords: Lepidoptera ; Sesiidae ; Synanthedon vespiformis ; Paranthrene tabaniformis ; Chamaesphecia empiformis ; Chamaesphecia tenthrediniformis ; Attractants ; Pheromones ; Clearwing moths ; Nemapogon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    Sex pheromone of the cone pyralid Dioryctria abietella (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 20-26 
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    ISSN: 1570-7458
    Keywords: Dioryctria abietella ; Cone pyralid ; Lepidoptera ; Pyralidae ; Sex pheromone, (Z,E)-9,11-tetradecadienyl acetate ; Single sensillum recordings ; Electroantennography ; Gas chromatography ; Mass spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'analyse en chromatographie gazeuse associée à une détection électroantennographique a montré que l'acétate (Z,E)-9,11-tétradécadiényle (Z,E)-9,11–14:Ac est l'un des composants de la phéromone de Dioryctria abietella Schiff (Lepid.: Pyralidae). Couplage chromatographie en phase gazeuse spectrometrie de masse a montré la présence d'acétate tétradécadiényle avec un spectre de masse et un indice de rétention identiques au Z,E-9,11–14:Ac Un récepteur cellulaire sensible à la fois au Z,E-9,11–14:Ac et à un extrait de la femelle a été identifié sous l'antenne du mâle. Les analyses des antennogrammes et de la cellule isolée ont étayé la caractérisation du composant de la phéromone comme étant Z,E-9,11–14:Ac. Un récepteur cellulaire additionnel sensible à l'acétate (Z.)-9-tétradécadiényle et à l'acétate (Z.E.)-9,12-tétradécadiényle a été trouvé sur l'antenne du mâle, mais il n'était pas activé par l'extrait de la femelle. Sur le terrain Z,E-9,11–14:Ac, présenté seul, attirait des nombres importants de mâles de D. abietella. L'addition de l'acétate (Z)-9-tétradécényle a inhibé l'attraction des mâles par les pièges.
    Notes: Summary Gas chromatographic analyses coupled with electro-antennographic detection indicated that (Z,E)-9,11-tetradecadienyl acetate (Z,E-9, 11–14:Ac) is a pheromone component of the cone pyralid Dioryctria abietella. Gas chromatographic-mass spectrometric analyses confirmed the presence of a tetradecadienyl acetate with mass spectrum and retention index identical to Z,E-9,11–14:Ac. A receptor cell sensitive to both Z,E-9,11–14:Ac and the female extract was identified on the male antenna. An additional receptor cell sensitive to (Z)-9-tetradecenyl acetate and (Z,E)-9,12-tetradecadienyl acetate was found on the male antenna but was not activated by the female extract. In the field Z,E-9,11–14:Ac presented alone attracted significant numbers of male D. abietella. Addition of (Z)-9-tetradecenyl acetate inhibited the attraction of males to traps.
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    Precocious metamorphosis and moulting deficiencies induced by an anti-JH compound, FMev in the fall webworm, Hyphantria cunea (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 65-70 
    Details
    ISSN: 1570-7458
    Keywords: Insect growth regulators ; Anti-juvenile hormone ; Fluoromevalonate ; Precocious metamorphosis ; Premature pupation ; Ecdysis ; Fall webworm ; Hyphantria cunea ; Lepidoptera ; Arctiidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Fluormevalosäure (FMev), bekannt als ein spezifischer Inhibitor der Biosynthese des Juvenilhormons (JH) in Insekten, wurde in 0,1–50 μg/Tier Dosis topikal an 3., 4. und 5. Larvenstadien von Hyphantria cunea getestet. Die Raupen wurden auf einem semi-synthetischen, künstlichen Nährboden bei 25° und unter Langtagsbedingungen (18 : 6 St., Licht/Dunkel) gezüchtet. Diese Verbindung rief drei verschiedene Typen spezifischer Reaktionen hervor: 1) verfrühte Metamorphose, 2) gehemmte Häutung und 3) verlängerte Larvenentwicklung. Vor der verfrühten Verpuppung wurde normales Verhalten beobachtet. Die Larven des 3. oder 4. Stadiums häuteten sich meist erst nach einem interkalaren Larvenstadium in verfrühte Puppen. Unter den drei Larvenstadien erwies sich das 5. Stadium gegen die Anti-JH-Verbindung am empfindlichsten. In allen getesteten Entwicklungsphasen wiesen die frisch gehäuteten Larven die höchste Empfindlichkeit gegen FMev auf. Nach der Häutung wurde stufenweises Absinken der FMev-Empfindlichkeit beobachtet, im 5. Larvenstadium verursachte die Verbindung jedoch selbst am letzten Tag des Wachstums zu einen relativ hohen Prozentzahl verfrühte Verpuppung. Eine zweite typische Wirkung von FMev war die Hemmung der Häutungsprozesse. Zwei grundlegende Stufen der Häutungsstörungen unterschieden sich voneinander: 1) Als Folge der Anwendung hoher Dosen von FMev konnte die Mehrzahl der Raupen die alte Larvenkutikula nicht öffnen und ging deswegen in kurzer Zeit zugrunde. 2) Bei Behandlung mit niedrigen Dosen der Anti-JH-Verbindung häuteten sich einige Hyphantria-Larven scheinbar normal; nach der Häutung waren aber alle unfähig, die normalen Bewegungen und die Nahrungsaufnahme fortzusetzen. Auch die vorzeitigen Puppenhäutungen wurden in meisten Fällen durch die Anti-JH-Behandlung gehemmt. Die morphogenetischen Wirkungen von FMev konnten durch eine topikale Behandlung mit Hydroprene, einem hochaktiven JH-Analogen, vollständig oder teilweise verhindert werden.
    Notes: Summary Fluoromevalonate (FMev, ZR-3516) known as an inhibitor of JH biosynthesis was topically applied in 0.1 to 50 μg/specimen doses to the 3rd, 4th, and 5th instar caterpillars of Hyphantria cunea Drury. The anti-JH compound induced 3 main types of specific responses: 1) precocious metamorphosis, 2) inhibition of ecdysis, and 3) prolongation of larval development. Precocious pupation was accompanied by behavioural events typical of normal pupation. Third and 4th instar larvae metamorphosed prematurely mostly with the intervention of an intercalary larval instar. The 5th instar exhibited the highest sensitivity to the anti-JH agent. Within each larval stage the freshly moulted insects proved to be the most susceptible to FMev. Afterwards, the incidence of morphogenetic reaction gradually decreased with age. In another fraction of Hyphantria larvae not responding with precocious pupation, FMev evoked varying degrees of ecdysial disturbance which always resulted in the death of caterpillars. In most cases the anti-JH compound inhibited the premature pupal moult, too, and these affected insects died as tanned pharate pupae. A complete or partial “rescue” from the effects of FMev was elicited, if simultaneously or subsequently, a single topical dose of a JH analogue, hydroprene was also administered.
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    Multivoltinism in Apanteles bignellii and the influence of weather on synchronisation with its host Euphydryas aurinia (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 155-162 
    Details
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Braconidae ; Lepidoptera ; Nymphalidae ; Apanteles bignellii ; Euphydryas aurinia ; Multivoltinism ; Synchronisation ; Weather
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung In einer Population von Euphydryas aurinia (Rottemburg) bei Oxford, England treten während einer Generation von E. aurinia drei Normalgenerationen von Apanteles bignellii Marshall auf. Jede Generation des Parasitoids kann charakterisiert werden durch das befallende Wirtsstadium und durch die aus einem Wirt schlüpfende Zahl Parasitoide. Parasitoide schlüpfen in Normalgenerationen aus dem 3., dem 4. und dem 6. Stadium des Wirts; in Ausnahmegenerationen schlüpfen sie aus dem 2. und 5. Stadium. Bis zu 70 Parasitoide können aus einer Altraupe (6. Stadium) schlüpfen und die Dauer dieses Stadiums kann bis auf 2 Wochen verlängert werden. Die Ausnahmegenerationen von A. bignellii aus Zweitlarven dürften aus Eiablagen in frühe Erstlarven stammen. Fünftlarven des Wirts, aus denen Parasitoide schlüpfen, sind ungewöhnlich klein und fressen nicht; sie dürften das Resultat sein eines Uebermasses von Apenteles-Eiern, die in frühe Viertlarven gelegt wurden. Die Synchronisation zwischen dem Parasitoiden und dem Wirt während der Zeit, da E. aurinia im Puppen-, Adult- oder Eistadium ist, wird aufrechterhalten durch ein verlängertes Coconstadium von Apanteles. Die Puppen des Parasitoiden entwickeln sich normal und die Adulten schlüpfen, bleiben aber bis 4 Wochen lang im Cocon, bevor sie sich eine Ausgangsöffnung machen. Das Wetter kann den Parasitierungsgrad der letzten Wirtsstadien beeinflussen. Wenn der Frühling kalt ist mit klarem Himmel, kann die Synchronisierung zwischen Parasitoiden, die aus Viertlarven des Wirts schlüpfen und potentiellen Fünft- und Sechtstlarven des Wirts schlecht werden. Die Entwicklung von Apanteles-Puppen wird durch die Umgebungstemperatur beeinflusst, während E. aurinia-Larven ihre Temperatur erhöhen, indem sie sich sonnen und deshalb rasch wachsen. Wenn die Parasitoiden unter solchen Bedingungen schlüpfen, sind die meisten potentiellen Wirte schon verpuppt und damit nicht mehr geeignet für die Parasitierung. Die Mechanismen der Synchronisation und der Wettereinfluss auf diese Vorgänge wird diskutiert.
    Notes: Abstract The gregarious endoparasite, Apanteles bignellii Marshall is specific to the nymphalid butterfly, Euphydryas aurinia (Rottemburg) in the British Isles. The synchronisation between host and parasitoid is described at a site near Oxford, England where both occur. Three regular generations of A. bignellii occur in one generation of the host in the studied population. Relevant features of the biology of A. bignellii and E. aurinia are described, including a method of distinguishing the number of Apanteles larval instars based upon shed cuticle remnants. Mechanisms for host-parasitoid synchronisation are outlined, especially a protracted parasitoid cocoon stage when the host is unavailable for attack during the chrysalis, adult and egg stages. Cool, but sunny weather conditions in spring can influence the degree of parasitisation experienced by final instar host caterpillars. The timing of adult A. bignellii emergence and subsequent attack on early instar hosts can lead to additional, partial, generations of parasitoids from second and fifth instar hosts.
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    Development of artificial diets for laboratory rearing of larvae of the spiny bollworm, Earias insulana (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 121-122 
    Details
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Noctuidae ; Earias insulana ; Bollworm ; Artificial diets ; Insect fecundity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00300911
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    tRNA-rRNA sequence homologies: Evidence for a common evolutionary origin? (1983)
    Springer
    Journal of molecular evolution 19 (1983), S. 420-428 
    Details
    ISSN: 1432-1432
    Keywords: Yeast ; E. coli ; tRNA ; rRNA ; Sequence homologies ; Evolution ; Origins ; Coding mechanism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Many tRNAs ofE. coli and yeast contain stretches whose base sequences are similar to those found in their respective rRNAs. The matches are too frequent and extensive to be attributed to coincidence. They are distributed without discernible pattern along and among the RNAs and between the two species. They occur in loops as well as in stems, among both conserved and non-conserved regions. Their distributions suggest that they reflect common ancestral origins rather than common functions, and that they represent true homologies.
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    URL: http://dx.doi.org/10.1007/BF02102317
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    Chloroplast and nuclear DNA fragments from Chlamydomonas promoting high frequency transformation of yeast (1983)
    Springer
    Current genetics 7 (1983), S. 473-480 
    Details
    ISSN: 1432-0983
    Keywords: ars sequences ; Yeast ; Chlamydomonas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A hybrid plasmid (pLG4) containing pBR325 and the yeast arg4 gene was constructed then used to isolate DNA fragments of Chlamydomonas able to promote high frequency transformation of yeast. Three plasmids containing EcoRI restriction fragments of chloroplast DNA and two plasmids containing Aval fragments of nuclear DNA were shown to support autonomous replication of plasmids in yeast. The three EcoRI fragments correspond to restriction fragments R4, R5 and R11 of native chloroplast DNA. These fragments are clustered in the physical map of chloroplast DNA constructed by Rochaix (1978). All isolated plasmids were shown to transform yeast at high frequency but the yeast transformants were quite unstable mitotically. Potential cloning sites are still available in the new plasmids which could be used as vectors in yeast and possibly in Chlamydomonas itself.
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    URL: http://dx.doi.org/10.1007/BF00377613
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    Recombinational analysis of oxi2 mutants and preliminary analysis of their translation products in S. cerevisiae (1983)
    Springer
    Current genetics 7 (1983), S. 225-233 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondria ; Intragenic recombination ; Mutant polypeptides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic and biochemical studies were performed with mutants allocated to the mitochondrial oxi2 gene. Recombinational analysis of 19 oxi2 mutants was performed using α and a mutant strains derived from the same genetic background. The frequencies of wild-type recombinants in oxi2 − × oxi2 − crosses varied from 0.002 to 17%. The map of oxi2 mutations constructed on the basis of these frequencies shows many internal inconsistencies. In the course of rho − deletion mapping five classes of oxi2 mutations were distinguished. The results of deletion analysis are in agreement with those of recombinational mapping. The analysis of mitochondrial translation products by SDS-polyacrylamide electrophoresis of 20 oxi2 mutants shows that 17 of them are connected with conspicuous changes of 22 kd polypeptide band corresponding to subunit III of cytochrome oxidase. At least four of them carried instead of subunit III clearly visible significantly shorter polypeptides (12.8 to 20.1 kd). These were, most likely, shorter fragments of subunit III resulting from chain termination mutations. Colinearity was observed between the lenght of new polypeptides and the positions of the respective mutations on the recombinational map. These data confirm hat oxi2 encodes subunit III of cytochrome oxidase and suggest that translation of the oxi2 gene is in the direction from V303 to V273.
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    URL: http://dx.doi.org/10.1007/BF00434894
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    A mutant of Saccharomyces cerevisiae defective in arginyl-tRNA-protein transferase (1983)
    Springer
    Current genetics 7 (1983), S. 285-288 
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    ISSN: 1432-0983
    Keywords: Arginyl-tRNA-Protein transferase ; Yeast ; Post-translational modification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A mutant of Saccharomyces cerevisiae deficient in arginyl-tRNA-protein transferase has been isolated. The responsible mutation designated ate1, was localized near the centromere of chromosome VII. It probably involves the structural gene for the transferase since residual enzyme activity in the mutant is temperature-sensitive.
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    Persistent heteroplasmic cells for mitochondrial genes in Saccharomyces cerevisiae (1983)
    Springer
    Current genetics 7 (1983), S. 489-492 
    Details
    ISSN: 1432-0983
    Keywords: Mitochondrial genes ; Yeast ; Vegetative segregation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genes in mitochondria and chloroplasts segregate rapidly during vegetative reproduction. Models to explain this vegetative segregation invoke either random segregation of organelle DNA molecules, or nonrandom segregation with random recombination events. All such models are basically stochastic. To look at vegetative segregation we took heteroplasmic (HET) cells containing mitochondrial mutations at the cap1, eryl and olil loci from several crosses. HETs were repeatedly selected and subcloned. Even after three to five successive subclonings (approximately 60–100 generations) some cells remained heteroplasmic. This confirms and extends previous observations of persistent HETs by Rank and Bech-Hansen (1972) and Forster and Kleese (1975), and by Bolen et al. (1980) for chloroplast genes in Chlamydomonas.
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    Isolation and characterization of yeast DNA repair genes (1983)
    Springer
    Current genetics 7 (1983), S. 85-92 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; RAD52 ; Cloning ; S1 and BAL31 Deletions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The RAD52 gene of Saccharomyces cerevisiae has previously been shown to be involved in both recombination and DNA repair. Here we report on the cloning of this gene. A plasmid containing a 5.9 kb yeast DNA fragment inserted into the BamH1 site of the YEp13 vector has been isolated and shown to complement the X-ray sensitive phenotype of the rad52-1 mutation. The rad52-1 cells containing the plasmid form larger colonies than similar cells having lost the plasmid. This plasmid has been shown not to complement either the U.V. sensitivity or the recombination defect of the E. coli recA mutation. From the insert various fragments have been subcloned into the YRp7 and YIp5 vectors. Integration events of two of the subclones have been genetically mapped to the chromosomal location of RAD52, indicating that the structural gene has been cloned. A 1.97 kb BamH1 fragment subcloned into YRp7 in one orientation complements the rad52-1 mutation, while the same fragment in the opposite orientation fails to complement. Various other subclones indicate that a BglII site, within the BamH1 fragment, is in the RAD52 gene. This BglII site has been deleted by Sl-nuclease digestion and the resulting deletion inactivates the RAD52 gene. BAL31 deletions from one end of a 1.9 kb Sal1-BamH1 fragment have been isolated; up to 0.9 kb can be deleted without loss of RAD52 activity, indicating that the RAD52 gene is approximately 1 kb or less in length.
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    Production and genetic analysis of yeast cybrids (1983)
    Springer
    Current genetics 7 (1983), S. 69-72 
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    ISSN: 1432-0983
    Keywords: Yeast ; Protoplast ; Cybrid ; Plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Data presented here demonstrate that fusion of protoplasts of two different haploid strains of Saccharomyces cerevisiae having the same mating type leads to the formation of “fusants” and “cytoplasmic hybrids”. The nuclear and cytoplasmic genome of a “fusant” combine those of the parent haploid strains. The “cytoplasmic hybrid” possesses the haploid genome of one parent and the combined cytoplasmic genomes of both. In mouse cells lines such products have been termed “cybrids” and this term has therefore been adopted here (Bunn and Wallace 1974).
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    Isolation and characterization of yeast DNA repair genes (1983)
    Springer
    Current genetics 7 (1983), S. 93-100 
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    ISSN: 1432-0983
    Keywords: Yeast ; RAD genes ; Cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plasmids that complement the yeast mutations rad50-1, rad51-1, rad54-3 and rad55-3 were obtained by transforming strains that carried a leu2 marker and the particular rad mutation, with YEp13 plasmids containing near random yeast DNA inserts. Integration of these plasmids or of fragments of these plasmids was accomplished. Genetic studies using the integrants established the presence of the genes RAD51, RAD54 and RAD55 in the respective plasmids. However, a BamHI subclone of the rad50-1 complementing plasmid failed to integrate at the RAD50 locus, indicating that no homology exists between this fragment and the RAD50 gene. A BamHI fragment from the RAD54 plasmid was shown to be internal to the RAD54 gene: its integration within a wild type copy of RAD54 causes the cell to become Rad−; its excision is X-ray inducible and restores the Rad+ phenotype. Since cells bearing a disrupted copy of RAD54 are able to survive, we conclude that this gene is not essential.
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    The effect of canavanine on the maintenance in yeast of chimeric plasmids containing portions of the 2-µm DNA plasmid (1983)
    Springer
    Current genetics 7 (1983), S. 363-367 
    Details
    ISSN: 1432-0983
    Keywords: Canavanine ; Yeast ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have found that the application of the amino acid analog canavanine to a culture of yeast cells transformed with chimeric plasmids based on the yeast 2-µm DNA plasmid increases the percentage of cells which have lost the transforming plasmid. This effect is found whether the plasmid carries the CAN1 sensitive allele and the yeast strain carries a can1 mutation confering resistance, or the plasmid contains no CAN1 allele and the yeast strain carries the wild-type CAN1 sensitive allele. Canavanine exerts this effect on yeast strains transformed with chimeric plasmids containing either a portion or the entire 2-µm DNA plasmid, yet canavanine does not appear to effect the maintenance of the native 2-µm DNA plasmid complement within the cell. The effect of canavanine on strains transformed with chimeric plasmids is the same whether or not the yeast strain also contains native 2-µm plasmid DNA. Neither the amino acid analog ethionine, the protein synthesis inhibitor cycloheximide, nor the DNA replication inhibitor hydroxyurea exhibit this effect. Some of the experimental results suggest that canavanine may be a curing agent rather than an agent which selects for spontaneous plasmid loss.
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    Polyamines enhance the efficiency of tRNA-mediated readthrough of amber and UGA termination codons in a yeast cell-free system (1983)
    Springer
    Current genetics 7 (1983), S. 421-426 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Polyamines ; Termination ; In vitro Translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of polyamines (spermidine and putrescine) on yeast suppressor tRNA-mediated readthrough of amber and UGA termination codons, in a homologous cell-free system, was examined. The efficiency of readthrough in a [psi+] lysate, mediated by exogenous suppressor tRNA, was significantly increased by polyamines as was the efficiency of endogenous UGA readthrough. The addition of polyamines, in the absence of exogenous suppressor tRNA, did not induce amber or ochre readthrough, nor could polyamines restore efficient termination readthrough in [psi−] lysates. It is concluded that polyamines interact with tRNA to increase the strength and specificity of the codon: anticodon interaction.
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    Chromosomal DNA sequences from Ustilago maydis promote autonomous replication of plasmids in Saccharomyces cerevisiae (1983)
    Springer
    Current genetics 7 (1983), S. 79-84 
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    ISSN: 1432-0983
    Keywords: ars sequences ; Ustilago ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary U. maydis chromosomal DNA sequences which promote the autonomous replication of plasmid YIp5 in S. cerevisiae YNN27 have been isolated and three of them characterised in some detail. Their properties are idential to yeast ars sequences in that plasmids containing them are maintained extrachromosomally as circular double-stranded DNA molecules, are mitotically unstable in yeast transformants and transform yeast at high frequencies. There is no sequence homology between the three U. maydis sequences and they are not reiterated in the U. maydis genome.
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    Studies on rapid reversible and non-reversible inactivation of fructose-1,6-bisphosphatase and malate dehydrogenase in wild-type and glycolytic block mutants of Saccharomyces cerevisiae (1983)
    Springer
    Archives of microbiology 134 (1983), S. 187-192 
    Details
    ISSN: 1432-072X
    Keywords: Carbon catabolite inactivation ; Yeast ; Malate dehydrogenase ; Fructose-1,6-bisphosphatase ; Glycolytic block mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Experimental conditions have been elaborated to test for reversibility of the malate dehydrogenase inactivation (E.C.1.1.1.37) after addition of glucose to derepressed yeast cells. Malate dehydrogenase inactivation was shown to be irreversible at all stages of inactivation. In contrast fructose-1,6-bisphosphatase inactivation (E.C.3.1.11) remained reversible for at least 30 min after addition of glucose. Rapid reversible inactivation of fructose-1,6-bisphosphatase and irreversible inactivation of malate dehydrogenase were additionally investigated in glycolytic block mutants. Normal inactivation kinetics were observed in mutants without catalytic activity of phosphoglucoseisomerase (E.C.5.3.1.9), phosphofructokinase (E.C.2.7.1.11), triosephosphate isomerase (E.C.5.3.1.1) and phosphoglycerate kinase (E.C.2.7.2.3). Hence, neither type of inactivation depended on the accumulation of any glucose metabolite beyond glucose-6-phosphate. Under anaerobic conditions irreversible inactivation was completely abolished in glycolytic block mutants. In contrast rapid reversible inactivation was independent of energy provided by respiration or fermentation. Reversibility of fructose-1,6-bisphosphatase inactivation was tested under conditions which prevented irreversible malate dehydrogenase inactivation. In these experiments, fructose-1,6-bisphosphatase inactivation remained reversible for at least 120 min, whereas reversibility was normally restricted to about 30 min. This indicated a common mechanism between the irreversible part of fructose-1,6-bisphosphatase inactivation and irreversible malate dehydrogenase inactivation.
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    Transient responses of yeast continuous cultures to qualitative changes in the nutrient supply. Induction and repression of the galactose pathway enzymes (1983)
    Springer
    Archives of microbiology 135 (1983), S. 115-119 
    Details
    ISSN: 1432-072X
    Keywords: Induction ; Catabolic repression ; galactose metabolism ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Induction and repression kinetics of alphagalactosidase, galactose uptake system and Leloir pathway enzymes were studied in chemostat cultures by changing the medium feed from glucose (11 mM) to glucose and galactose (11 mM; 17 mM respectively) in the induction experiments; and from galactose (11 mM) or (111 mM) to galactose plus glucose (83 mM) in the repression experiments. Basal levels of alpha-galactosidase and glucose uptake could be estimated in glucose-limited yeast cells, but it was not possible to detect any glactose pathway enzyme activity. In the repression experiments under galactose-limited or galactose-sufficient yeast cells, alpha-galactosidase and galactokinase decayed with K d=-0.21h-1=-D; that is, synthesis of these enzymes ceased (catabolite repression). In contrast transferase and epimerase activities and galactose uptake, decreased with K d values of-0.33 and-0.54h-1, showing that these activities were also subject to catabolite inactivation.
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    In vivo 31P NMR studies on the role of the vacuole in phosphate metabolism in yeasts (1983)
    Springer
    Archives of microbiology 134 (1983), S. 270-275 
    Details
    ISSN: 1432-072X
    Keywords: Candida utilis ; Brettanomyces intermedius ; Yeast ; Glycolysis ; Vacuole ; Cytoplasm ; Phosphate compartmentation ; Phosphate transport ; Polyphosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 31P NMR was used to study the dynamics of phosphate pools during substrate utilization by aerobic and anaerobic suspensions of the yeast Candida utilis and by aerobic suspensions of the yeast Brettanomyces intermedius. In both yeast, the cytoplasmic pH was monitored; in C. utilis also the vacuolar pH could be measured. When glucose was used as a substrate for C. utilis, the vacuolar store of inorganic phosphorus (both orthophosphate and polyphosphate) was mobilized to replenish cytoplasmic phosphate which had become very low due to the build-up of high sugar phosphate levels. The hydrolysis of polyphosphate was glucose-dependent; it did not occur with ethanol as the substrate. After glucose depletion resynthesis of polyphosphate occurred only under aerobic conditions; anaerobic C. utilis cells continued to hydrolyze vacuolar polyphosphate. This difference between the aerobic and anaerobic suspension could be related to differences in cellular ATP levels. When ethanol was employed as a substrate, both Candida utilis and Brettanomyces intermedius exhibited a substantial increase in polyphosphate levels. These observations suggested a dual role for polyphosphate in yeasts both as a phosphate and an energy store. The cytoplasmic pH in C. utilis displayed characteristic responses to metabolic changes during glucose degradation. B. intermedius experienced a strong cytoplasmic acidification upon ethanol utilization due to acetic acid formation. The mechanism of transport of Pi across the vacuolar membrane in C. utilis appeared to be different from that reported for the plasma membrane.
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    URL: http://dx.doi.org/10.1007/BF00407801
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    Sex pheromone components of the European goat moth,Cossus cossus (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 191-200 
    Details
    ISSN: 1573-1561
    Keywords: Sex pheromone ; Cossus cossus ; Lepidoptera ; Cossidae ; (Z)-5-dodecenyl acetate ; (Z)-5-dodecenol ; (Z)-5-tetradecenyl acetate ; (Z)-3-decenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Female tip washings of the European goat moth,Cossus cossus L., were found to contain the following compounds that are structurally similar to known pheromone components of Lepidoptera (%): decyl acetate (1.5), (Z)-5-dodecenol (1), (Z)-5-dodecenyl acetate (66), dodecyl acetate (12), (Z)-5-tetradecenyl acetate (14), tetradecyl acetate (4), and hexadecyl acetate (1.5). The washes contained an average of 125 ng of (Z)-5-dodecenyl acetate per female equivalent. EAG responses to nanogram amounts of the identified products indicated that (Z)-5-dodecenyl acetate was the most potent olfactory stimulant followed by (Z)-5-tetradecenyl acetate and (Z)-5-dodecenol, whereas the saturated acetates caused only weak depolarization. A strong EAG response was also recorded for (Z)-3-decenyl acetate, which was not detected in the female tip washings. Field results showed that (Z)-5-dodecenyl acetate was essential for the attraction of the males and that (Z)-3-decenyl acetate improved the attractiveness of (Z)-5-dodecenyl acetate alone or in combination with (Z)-5-tetradecenyl acetate.
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    URL: http://dx.doi.org/10.1007/BF00988036
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    Synthesis of (Z)-13-hexadecen-11-yn-1-yl acetate Major component of sex pheromone of the processionary moth (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 219-223 
    Details
    ISSN: 1573-1561
    Keywords: Synthesis ; sex pheromone ; processionary moth ; Thaume-topoea pityocampa ; Lepidoptera ; Notodontidae ; (Z)-13-hexadecen-11-yn-1-yl acetate ; acetylene ; carbocupration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The synthesis of the major component of the sex pheromone of the processionary mothThaumetopoea pityocampa is described. The synthesis uses the carbocupration of acetylene, followed by the coupling with the appropriate 1-iodoalkyne. This synthetic pheromone was shown to be of 98.8%Z purity.
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    URL: http://dx.doi.org/10.1007/BF00988039
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  • 89
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    Sex pheromone components ofEuxoa drewseni Chemical identification, electrophysiological evaluation, and field attractancy tests (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 335-346 
    Details
    ISSN: 1573-1561
    Keywords: Euxoa drewseni ; Chorizagrotis thanatologia ; Lepidoptera ; Noctuidae ; sex pheromone ; attractant inhibitor ; parapheromone ; (Z)-5-dodecenyl acetate ; (Z)-7-dodecenyl acetate ; (Z)-7-tetradecenyl acetate ; (Z)-7-pentadecenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Eleven compounds structurally similar to known lepidopterous pheromone components were identified in the extract from 18 calling female moths ofEuxoa drewseni (Staudinger). The identifications were done by gas chromatography-mass spectrometry and high-resolution gas chromatography with flame ionization and electroantennographic detectors simultaneously. Detector antennae were from five species of moths. In the field, male moths were specifically attracted to a three-component blend of dodecyl, (Z)-5-dodecenyl, and (Z)-7-tetradecenyl acetates in a ratio of 2∶6∶1. This blend at 1000 μg/rubber septum dispenser is recommended as a trap bait for monitoring purposes. Low concentrations of (Z)-7-dodecenyl acetate or (Z)-7-tetradecenol inhibited the attraction of moths to the three-component blend. (Z)-7-Pentadecenyl acetate functioned as a parapheromone in place of (Z)-7-tetradecenyl acetate in the pheromone blend, and they appear to react via the same antennal receptor.
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    URL: http://dx.doi.org/10.1007/BF00988453
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  • 90
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    Apparent long-term bodily contamination by disparlure, the gypsy moth (Lymantria dispar) Attractant (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 33-37 
    Details
    ISSN: 1573-1561
    Keywords: Disparlure ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; gypsy moth ; pheromone persistence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Positive attraction of male gypsy moths to the body of the author, now four years after his last known direct contact with disparlure, the synthetic pheromone, is documented. A designed test showed that moths responded to him in highly significantly greater numbers than to others who had had less or no previous exposure to the insect and / or disparlure. The data present persuasive evidence of bodily contamination with disparlure and suggest that the material is very persistent.
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    URL: http://dx.doi.org/10.1007/BF00987768
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  • 91
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    Triunsaturated hydrocarbons, sex pheromone components ofCaenurgina erechtea (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 1413-1423 
    Details
    ISSN: 1573-1561
    Keywords: Behavior ; Caenurgina erechtea (Cramer) ; forage looper ; Lepidoptera ; Noctuidae ; sex pheromone ; (Z,Z,Z)-3,6,9-eicosatriene ; (Z,Z,Z)-3,6,9-heneicosatriene ; flight tunnel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (Z,Z,Z)-3,6,9-Eicosatriene and (Z,Z,Z)-3,6,9-heneicosatriene have been identified as components of the sex pheromone of the noctuid,Caenurgina erechtea (Cramer), the forage looper. Structural assignments were made on the basis of spectroscopic and chromatographic data and were confirmed by comparison with synthetic material. Flight tunnel behavioral studies demonstrated that either component, when tested individually, would elicit wing fanning responses in males; however, mixtures of the two components increased this response and were essential for initiation of upwind flight and landing. In field experiments, traps baited with either component alone captured few or no adult forage looper males while those baited with both components captured several target males.
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    URL: http://dx.doi.org/10.1007/BF00990747
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  • 92
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    Identification of a sex pheromone produced by female velvetbean caterpillar moth (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 645-656 
    Details
    ISSN: 1573-1561
    Keywords: Sex pheromone ; 3,6,9-heneicosatriene ; 3,6,9-eicosatriene ; velvetbean caterpillar ; Anticarsia gemmatalis Hübner ; Lepidoptera ; Noctuidae ; attractant ; hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A sex pheromone produced by female velvetbean caterpillar moths,Anticarsia gemmatalis Hübner, that attracts conspecific males was isolated and identified as a blend of (Z,Z,Z)-3,6,9-eicosatriene and (Z,Z,Z)-3,6,9-heneicosatriene in a ratio of ca. 5∶3, respectively, when combined. The synthesized compounds elicited responses by velvetbean caterpillar moth males equivalent to those elicited by females in both laboratory wind tunnel bioassays and field trapping experiments.
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    URL: http://dx.doi.org/10.1007/BF00990414
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    Effect of soybean phytoalexins on the herbivorous insects mexican bean beetle and soybean looper (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 657-672 
    Details
    ISSN: 1573-1561
    Keywords: Pseudoplusia includens ; soybean looper ; Lepidoptera ; Noctuidae ; Epilachna varivestis ; Mexican bean beetle ; Coleoptera ; Coccinellidae ; feeding preferences ; nutrition ; food utilization ; host-plant resistance ; induced resistance ; glyceollin ; isoflavonoids ; soybean ; phytoalexins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Effects of soybean phytoalexins on the feeding of the soybean looper and Mexican bean beetle were investigated to test the hypothesis that phytoalexins might be a defense mechanism of plants against insects as well as against pathogens. Short-term behavioral responses to the phytoalexins were analyzed using dual-choice tests with phytoalexin-rich and phytoalexin-poor (control) tissues. Phytoalexin production was elicited with ultraviolet radiation. Results from the dual-choice tests indicated that 6th instar soybean looper larvae fed equally on the control and phytoalexin-rich tissues. Feeding by adult and 4th instar Mexican bean beetles, however, was strongly deterred by the phytoalexins as evidenced by “single-bite” mandible scars on the phytoalexin-rich cotyledon discs. Nutritional effects of the isoflavonoid phytoalexin glyceollin on early instar soybean looper larvae were tested by incorporating the phytoalexin into an artificial medium at a level of 1% dry weight (0.15% fresh weight). The larvae were reared for 7 days from emergence on diets of control and glyceollin-containing media. Although survival on the glyceollin diets was initially less than on the control diets, under the experimental conditions glyceollin had no significant effect on the growth, development, or subsequent survival of the larvae. Efficiency of food utilization (ECI) was reduced, indicating that the phytoalexins may be a mild digestibility-reducing factor for the loopers. Implications of the results for host-plant resistance are discussed.
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    URL: http://dx.doi.org/10.1007/BF00988774
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    Identification of sex pheromone components of the female driedfruit moth,Vitula edmandsae serratilineella, and a blend for attraction of male moths (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 785-801 
    Details
    ISSN: 1573-1561
    Keywords: Driedfruit moth ; Vitula edmandsae serratilineella ; Lepidoptera ; Pyralidae ; Phycitinae ; sex pheromone ; attractant inhibitor ; (Z)-9,(E)-12-tetradecadienol ; (Z)-9,(E)-12-tetradecadienal ; alcohol ; aldehyde
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Sixteen pheromone-like compounds were identified in abdomen tip washes and excised abdomen tip extracts of calling females of driedfruit moth,Vitula edmandsae serratilineella Ragonot. Identifications were by high-resolution gas chromatography-mass spectrometry, and gas chromatography with flame ionization and electroantennographic detectors. Male moths were attracted to a blend of (Z)-9,(E)-12-tetradecadienol and (Z)-9,(E)-12-tetradecadienal in a ratio of 100∶1 at 500 μg/rubber septum dispenser, which is recommended for monitoring purposes. Low concentrations of (Z)-9-tetradecenol (0.5%) and (Z)-9-tetradecenal (0.1%) may be beneficial for the attraction of males, but 1–2% of (E)-9,(E)-12- or (Z)-9,(Z)-12-tetradecadienol, or (Z)-9,(E)-12-tetradecadienyl acetate inhibited their attraction. Gravid female moths were attracted to traps that captured large numbers of males. Females may be attracted to male hairpencil or forewing gland secretions emitted near the traps or that accumulate in the traps.
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    URL: http://dx.doi.org/10.1007/BF00988783
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    Initial field trials with the synthetic sex pheromone of the processionary mothThaumetopoea pityocampa (Denis and Schiff.) (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 85-93 
    Details
    ISSN: 1573-1561
    Keywords: Sex attractant ; sex pheromone ; processionary moth ; Thaumetopoea pityocampa ; Lepidoptera ; Notodontidae ; (Z)-13-hexadecen-11-ynyl acetate ; field test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Synthetic (Z)-13-hexadecen-11-ynyl acetate has been shown to be highly active in catchingThaumetopoea pityocampa (Denis and Schiff.) males in field trials carried out in different parts of Spain. A variety of formulations containing antioxidants or solid paraffin were tested. Formulations containing 3 and 10% ofE isomer showed a decrease of activity compared with those prepared with pureZ isomer. Dodecyl acetate, also found in the virgin female gland, did not show any synergistic effect when tested in a 9∶1 mixture with the synthetic pheromone. The product exhibited a remarkable persistence of activity under the field conditions even in the absence of stabilizer.
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    URL: http://dx.doi.org/10.1007/BF00987772
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  • 96
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    Spodoptera exigua: Capture of feral males in traps baited with blends of pheromone components (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 95-104 
    Details
    ISSN: 1573-1561
    Keywords: Spodoptera exigua ; Lepidoptera ; Noctuidae ; pheromone ; rubber septa ; (Z,E)-9,12-tetradecen-1-ol acetate ; (Z)-9-tetradecen-1-ol ; beet armyworm ; trapping sticky trap
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Field tests were conducted with various blends of acetates and alcohols previously identified as components of the sex pheromone for the beet armyworm (BAW),Spodoptera exigua (Hübner). The compounds were formulated on rubber septa and placed in sticky traps positioned in fields populated withAmaranthus sp., a favored host of this species. Moth captures were highest in traps baited with septa containing a blend of 0.1 mg (Z,E)-9,12-tetradecadien-1-ol acetate (Z9,E12–14∶Ac) and 0.01 mg(Z)-9-tetradecen-1-ol (Z9–14∶OH). Additionally, we confirmed that all combinations of acetates tested alone captured significantly fewer males than blends containing a 10∶1 ratio ofZ9,E12–14∶Ac andZ9–14∶OH. A 10∶1 formulation ofZ9,E12–14∶Ac andZ9–14∶OH in hollow fibers was also attractive to feral BAW males.
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    URL: http://dx.doi.org/10.1007/BF00987773
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  • 97
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    Field trapping of diamondback mothPlutella xylostella using an improved four-component sex attractant blend (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 113-118 
    Details
    ISSN: 1573-1561
    Keywords: Diamondback moth ; Plutella xylostella ; Crymodes devastator ; Lepidoptera ; Yponomeutidae ; Noctuidae ; sex attractant ; (Z)-11-hexadecenyl acetate ; (Z)-11-hexadecen-1-ol ; (Z)-11-hexadecenal ; (Z)-9-tetradecenyl acetate ; (Z)-9-tetradecen-1-ol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In addition to three known sex lure components [(Z)-11-hexadecenyl acetate, (Z)-11-hexadecenal, and (Z)-11-hexadecenol], (Z)-9-tetradecenyl acetate was field-proven as a trace coattractant for malePlutella xylostella, with an optimal content below 0.01% in blends. This potent four-component lure for diamondback males also attractedCrymodes devastator males, and in this respect is not different in its attractancy from virgin diamondback females. Replacement of (Z)-9-tetradecenyl acetate in the four component lure with (Z)-9-tetradecen-1-ol, at the level of 10% of the total lure mixture, did not alter its attractancy for diamondback males, but it did inhibit attraction ofCrymodes devastator. The status of biologically active components as possible sex pheromones or para-pheromones is discussed.
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    URL: http://dx.doi.org/10.1007/BF00987775
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    Chemical basis for host selection byHemileuca oliviae (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 1425-1437 
    Details
    ISSN: 1573-1561
    Keywords: Range caterpillar ; Hemileuca oliviae ; Lepidoptera ; Saturniidae ; C4 plants ; C3 plants ; tannins ; grasses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract When provided a choice between grass species with C3 or C4 photosynthetic pathways, larvae of range caterpillar,Hemileuca oliviae Cockerell, selected C4 grasses. The basis for host selection was examined by conducting analyses of moisture, crude protein, total available carbohydrate, sucrose, glucose, astringency, condensed tannin, silica, and pubescence of 14 grass species, and correlating host plant chemical characteristics with host preference. Most of the variation in host preference was explained by tannin characteristics (astringency and condensed tannin); C3 grass species had significantly higher tannin levels than C4 species.
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    URL: http://dx.doi.org/10.1007/BF00990748
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    Identification of two components of the sex pheromone of the moth,Epiphyas postvittana (Lepidoptera, Tortricidae) (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 503-512 
    Details
    ISSN: 1573-1561
    Keywords: Epiphyas postvittana ; Lepidoptera ; Fortricidae ; lightbrown apple moth ; sex pheromone ; (E)-11-tetradecen-1-yl acetate ; (E,E)-9,11-tetradecadien-1-yl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Two compounds, (E)-11-tetradecen-1-yl acetate and (E,E)-9,11-tetradecadien-1-yl acetate, have been identified in extracts of females of the lightbrown apple moth,Epiphyas postvittana (Walker). The two compounds are active as a coalitive pair and are present in extracts of females in a ratio of about 20∶1.
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    URL: http://dx.doi.org/10.1007/BF00990222
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    The role of iridoid glycosides in host-plant specificity of checkerspot butterflies (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 475-493 
    Details
    ISSN: 1573-1561
    Keywords: Iridoid glycoside ; catalpol ; Scrophulariaceae ; Euphydryas ; checkerspot ; host-plant specificity ; Lepidoptera ; Nymphalidae ; coevolution ; insect-plant interaction ; chemical ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The potential role of iridoid glycosides as feeding stimulants forEuphydryas chalcedona larvae was examined in three laboratory experiments. The first experiment examined larval behavior in choice tests between an artificial diet with no additives (AD) and an artificial diet with the iridoid glycoside, catalpol, added (AD + I) in one group; and AD and AD plus a crude extract from which the iridoid glycoside catalpol was crystallized (AD + Ex) in the second group. The larvae were found more often on AD + I or AD + Ex. The second experiment quantified larval consumption of artificial diets when given a choice of AD or AD + I, and AD or AD + Ex, and showed that larvae ate significantly more AD + I or AD + Ex than AD. The third experiment compared growth and survival on six diets: AD; AD + I; artificial diet with dried, ground upScrophularia californica leaves (AD + S); artificial diet with dried, ground upPlantago lanceolata leaves (AD + P);S. californica leaves (S); andP. lanceolata leaves (P). Growth was best onS. californica leaves, and survival was highest onS. californica andP. lanceolata leaves. There were no differences in growth rate or survival between AD andAD + I. Thus, iridoid glycosides serve as feeding attractants and stimulants for larvae ofEuphydryas chalcedona and are suggested as the basis of radiation in butterflies of the genusEuphydryas.
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    URL: http://dx.doi.org/10.1007/BF00990220
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