ALBERT

Description: Helmet streamers on the sun were observed to be the site of coronal mass ejections, dynamic events that eject coronal plasma and magnetic fields into the solar wind. A two dimensional (azimuthally symmetric) helmet streamer configuration was developed by computing solutions of the time dependent magnetohydrodynamic (MHD) equations, for a specified magnetic flux distribution on the sun. The helmet streamer is not symmetric about the equator. The evolution of the configuration, when differential rotation is applied, was investigated. It was found that after many rotations the configuration does not reach a steady state, but disrupts recurrently with the ejection of a plasmoid. These results suggest that differential rotation may be one of the mechanisms by which mass ejections are initiated.

Description: The development of an integrated approach to the modeling of forest dynamics encompassing submodels of forest growth and succession, soil processes and radiation interactions, is reported. Remote sensing technology is a key element of this study in that it provides data for developing, initializing, updating, and validating the models. The objectives are reviewed, the data collected and models in use are discussed, and a framework for studying interactions between the forest growth, soil process and energy interaction components, is described. Remote sensing technology used in the study includes optical and microwave field, aircraft and satellite borne instruments. The types of data collected during intensive field and aircraft campaigns included bidirectional reflectance, thermal emittance and multifrequency, multipolarization synthetic aperture radar backscatter. Synthetic imagery of derived products such as forest biomass and NDVI (Normalized Difference Vegetative Index), and collections of ground data are being assembled in a georeferenced data base. These data are used to drive or test multidiscipline simulations of forested ecosystems. Enhancements to the modeling environment permit considerable flexibility in configuring simulations and selecting results for reporting and graphical display.

Description: The quantitative interpretation of satellite observations requires the use of mathematical tools to extract the desired information on terrestrial environments from the radiation data collected in space. A whole range of approaches can be pursued, from the development of models capable of explaining the nature of the physical signal being measured and of characterizing the state of the system under observation, to the empirical correlations between the variables of interest and the space measurements. The premises and implications of these approaches are outlined, paying special attention to the mathematical and numerical requirements. The role and specific applications of empirical bidirectional reflectance models is also discussed, even though these models do not contribute to the understanding of the theory of radiation transfer or to the assessment of the variables of interest. The advantages and drawbacks of these various approaches and the research priorities for the next few years are discussed in the context of the planned availability of new sensors.

Description: Management of crop residues, the portion of a crop left in the field after harvest, is an important conservation practice for minimizing soil erosion and for improving water quality. Quantification of crop residue cover is required to evaluate the effectiveness of conservation tillage practices. Methods are needed to quantify residue cover that are rapid, accurate, and objective. The fluorescence of crop residue was found to be a broadband phenomenon with emission maxima at 420 to 495 nm for excitations of 350 to 420 nm. Soils had low intensity broadband emissions over the 400 to 690 nm region for excitations of 300 to 600 nm. The range of relative fluorescence intensities for the crop residues was much greater than the fluorescence observed of the soils. As the crop residues decompose their blue fluorescence values approach the fluorescence of the soil. Fluorescence techniques are concluded to be less ambiguous and better suited for discriminating crop residues and soils than reflectance methods. If properly implemented, fluorescence techniques can be used to quantify, not only crop residue cover, but also photosynthetic efficiency in the field.

Description: The importance of the measurement of wind fields is discussed. Wind regime data can be used to infer the amount and type of wind induced (aerolian) transport of sand and dust, or to establish global circulation models, for example on other planets. Since local measurements are costly and often impossible, it is desired to infer such data from remotely sensed information. A potential mechanism for remotely inferring the wind regime by using synthetic aperture radar data to describe the roughness of the surface is described. A project to estimate the practicality of using such a mechanism is described. An experiment that extends the mechanism to vegetated sites, where the goal is to measure potential for erosion, is reported.

Description: Surface reflectance is required to quantitatively investigate molecular absorption and particle scattering properties of materials on the Earth's surface. Atmospheric aerosol optical depth, surface pressure and water vapor are required to constrain a radiative transfer code for the inversion of measured spectral radiance to apparent surface reflectance. A suite of algorithms using nonlinear least squares fitting techniques are described that directly estimate these atmospheric parameters from spectral radiance measured by the Airborne Visible/Infrared Imaging Spectrometer (AVIRIS). The derived atmospheric parameters are used to constrain a radiative transfer code for the inversion of the imaging spectrometer radiance to apparent reflectance. The derived apparent reflectance is validated with respect to in situ measurement on the same target.

Description: Investigations designed to study land surface hydrologic-atmospheric interactions, showing the potential of L band passive microwave radiometry for measuring surface soil moisture over large areas, are discussed. Satisfying the data needs of these investigations requires the ability to map large areas rapidly. With aircraft systems this means a need for more beam positions over a wider swath on each flightline. For satellite systems the essential problem is resolution. Both of these needs are currently being addressed through the development and verification of Electronically Scanned Thinned Array Radiometer (ESTAR) technology. The ESTAR L band radiometer was evaluated for soil moisture mapping applications in two studies. The first was conducted over the semiarid rangeland Walnut Gulch watershed located in south eastern Arizona (U.S.). The second was performed in the subhumid Little Washita watershed in south west Oklahoma (U.S.). Both tests showed that the ESTAR is capable of providing soil moisture with the same level of accuracy as existing systems.

Description: A weather resistant automatic scanning Sun photometer system is assessed and demonstrated as practical for measurements of aerosol concentrations and properties at remote sites. Interfaced with a transmitter using the Geostationary Data Collection System (GDCS), the data are processed in near real time. The processing allows a time dependence of the aerosols and water vapor and an ongoing assessment of the health and calibration of the instruments. The system's automatic data acquisition, transmission, and processing offer immediate application to atmospheric monitoring and modeling on a regional to global scale and validation of satellite retrievals. It is estimated that under normal circumstances the retrieved aerosol optical thickness has a network wide accuracy of +/- 0.02 from 340 nm to 1020 nm, water vapor +/- 0.2 cm and size distribution from 0.1 to 3 micrometers.

Description: The ground-based observing facilities of the National Solar Observatory (NSO) are reviewed from the perspective of joint observations with SOHO. A specific proposal is presented for observations of the HE-I 1083.0 nm line with the NASA/NSO spectromagnetograph and He 10830 video filtergraph/magnetograph in coordination with ultraviolet sensitive instruments on SOHO. The first task will be to look for associations of low-temperature transition-region lines with He 1083 nm absorption to investigate Andretta's conjecture, i.e. that the He 1083 nm line is formed in two layers where extreme ultraviolet radiation produced both in the low-temperature transition region (the upper layer) and in the surrounding corona products - a lower layer of absorption in the upper chromosphere.

Description: The white light coronograph (WLC) on Skylab provided an opportunity to study the corona at high spatial and temporal resolution. The spatial resolution of the instrument was approximately 25 cm with images taken approximately one per min. One set of images taken over a 10 min period was digitized, providing ten high spatial resolution images for analysis. The progress in data processing techniques available at the time was not sufficient to permit a reliable study of the fine structure in these images. Using current techniques an investigation of the sizes and lifetimes of the smallest scale features in the data was carried out. A preliminary analysis of an area between 2 and 3 Ro was completed. The results show that very narrow rays extend from at least 2 to 3 Ro. The narrowest of these rays has a thickness of approximately 75 cm. The contrast is so low that they are very close to the noise limit of the data. Most of the rays observed become unrecognizable after 10 min, although some remain visible over the entire time. Some notion seems to be detectable in the fine structure rays, but analysis of more frames will be needed to quantify these results.

Description: As part of a global program to validate the ocean surface sensors on board ERS-1, a joint experiment on the Grand Banks of Newfoundland was carried out in Nov. 1991. The principal objective was to provide a field validation of ERS-1 Synthetic Aperture Radar (SAR) measurement of ocean surface structure. The NASA-P3 aircraft measurements made during this experiment provide independent measurements of the ocean surface along the validation swath. The Radar Ocean Wave Spectrometer (ROWS) is a radar sensor designed to measure direction of the long wave components using spectral analysis of the tilt induced radar backscatter modulation. This technique greatly differs from SAR and thus, provides a unique set of measurements for use in evaluating SAR performance. Also, an altimeter channel in the ROWS gives simultaneous information on the surface wave height and radar mean square slope parameter. The sets of geophysical parameters (wind speed, significant wave height, directional spectrum) are used to study the SAR's ability to accurately measure ocean gravity waves. The known distortion imposed on the true directional spectrum by the SAR imaging mechanism is discussed in light of the direct comparisons between ERS-1 SAR, airborne Canadian Center for Remote Sensing (CCRS) SAR, and ROWS spectra and the use of the nonlinear ocean SAR transform.

Description: Terrain slopes, which can be measured with Synthetic Aperture Radar (SAR) interferometry either from a height map or from the interferometric phase gradient, were used to calculate the local incidence angle and the correct pixel area. Both are required for correct thematic interpretation of SAR data. The interferometric correlation depends on the pixel area projected on a plane perpendicular to the look vector and requires correction for slope effects. Methods for normalization of the backscatter and interferometric correlation for ERS-1 SAR are presented.

Description: The relationship between the gradient of the interferometric phase and the terrain slope, which, it is thought, would allow a derivation of the terrain slopes without phase unwrapping, is presented. A linear relationship between the interferometric phase gradient and the terrain slopes was found. A quantitative error analysis showed that only very small errors are introduced by these approximations for orbital Synthetic Aperture Radar (SAR) geometries. An example of a slope map for repeat pass interferometry from ERS-1 SAR data is given. A number of direct and indirect applications of the terrain slope are indicated: erosion and avalanche hazard studies, radiometric calibration of SAR data, and normalization of the interferometric correlation coefficient.

Description: Filaments, flare sprays, prominences and 'post-flare' loops are familiar to H alpha observers in their frequent appearances 'in absorption', dark against the chromospheric background or plages. Observations of the X-ray corona are generally interpreted as due to emission via optically thin thermal bremsstrahlung. Several cases of X-ray coronal structures in Yohkoh images, due to high opacity, absorbing matter in coronograph loops, are presented. The presence of the absorbing matter, mixed with emitting matter, complicates inference of physical parameters such as emission measures in X-ray sources. In the case of well defined features, absorption provides an opportunity to infer density. Quantitative estimates of the attenuation due to the absorption in example features are presented.

Description: The causal association of major solar particle events seen at earth with coronal mass ejections (CME's), and not with solar flares, is discussed. Evidence that led to the demise of the flare dominated paradigm for major solar energetic particle events are described. The possibility of distinguishing particles from impulsive and gradual events using only observations is described. Particle acceleration at the CME level is discussed. Multi-spacecraft observations of CME events are described. Concerning the interplanetary CME, bidirectional proton events are discussed. Conclusions from progress in understanding the characteristics of solar energetic particles and their relation to the physical mechanisms of acceleration are given.

Description: Two dimensional magnetohydrodynamic simulations of the distortion of a magnetic flux tube, accelerated through ambient solar wind plasma, are presented. Vortices form on the trailing edge of the flux tube, and couple strongly to its interior. If the flux tube azimuthal field is weak, it deforms into an elongated banana-like shape after a few Alfven transit times. A significant azimuthal field component inhibits this distortion. In the case of magnetic clouds in the solar wind, it is suggested that the shape observed at 1 AU was determined by distortion of the cloud in the inner heliosphere. Distortion of the cloud beyond 1 AU takes many days. It is estimated that effective drag coefficients slightly greater than unity are appropriate for modeling flux tube propagation. Synthetic magnetic field profiles as would be seen by a spacecraft traversing the cloud are presented.

Description: Since radio propagation measurements using either natural or spacecraft radio signals are used for probing the solar wind in the vicinity of the sun, they represent a key tool for studying the interplanetary consequences of solar structure and dynamic phenomena. New information on the near sun consequences was obtained from radio scintillation observations of coherent spacecraft signals. The results covering density fluctuations, fractional density fluctuations, coronal streamers, heliospheric current sheets, coronal mass ejections and interplanetary shocks are reviewed. A joint ICE S-band (13 cm wavelength) Doppler scintillation measurement with the SOHO white-light coronograph (LASCO) is described.

Description: Research on the use of active microwaves in remote sensing, presented during plenary and poster sessions, is summarized. The main highlights are: calibration techniques are well understood; innovative modeling approaches have been developed which increase active microwave applications (segmentation prior to model inversion, use of ERS-1 scatterometer, simulations); polarization angle and frequency diversity improves characterization of ice sheets, vegetation, and determination of soil moisture (X band sensor study); SAR (Synthetic Aperture Radar) interferometry potential is emerging; use of multiple sensors/extended spectral signatures is important (increase emphasis).

Description: Crop residues, the portion of the crop left in the field after harvest, can be an important management factor in controlling soil erosion. Methods to quantify residue cover are needed that are rapid, accurate, and objective. Scenes with known amounts of crop residue were illuminated with long wave ultraviolet (UV) radiation and fluorescence images were recorded with an intensified video camera fitted with a 453 to 488 nm band pass filter. A light colored soil and a dark colored soil were used as background for the weathered soybean stems. Residue cover was determined by counting the proportion of the pixels in the image with fluorescence values greater than a threshold. Soil pixels had the lowest gray levels in the images. The values of the soybean residue pixels spanned nearly the full range of the 8-bit video data. Classification accuracies typically were within 3(absolute units) of measured cover values. Video imaging can provide an intuitive understanding of the fraction of the soil covered by residue.

Description: Global study of land surface properties uses AVHRR channels 1 and 2, but channel 3 may be of interest, although its use requires preprocessing. It consists of both a reflective part and an emissive part, the former can be derived from T3, T4 and T5. Since the water vapor affects channel 3, its content is retrieved from the channel 4 and 5 using the split window technique. A formula of reflective part retrieval at 3.75 micrometers is tested in the case of sunglint observations where the emissivities of channels 4 and 5 can be set to the unity. The formula is adapted and validated to land surface using the FIFE-87 data set. Preliminary applications of the reflectance at 3.75 micrometers to the studies of surface properties retrieval, aerosol retrieval over land, and desertic aerosol retrieval, are addressed.

Description: The polarization of the sunlight scattered by atmospheric aerosols or cloud droplets and reflected from ground surfaces or plant canopies may convey much information when used for remote sensing purposes. The typical polarization features of aerosols, cloud droplets, and plant canopies, as observed by ground based and airborne sensors, are investigated, looking especially for those invariant properties amenable to description by simple models when possible. The question of polarization measurements from space is addressed. The interest of such measurements for remote sensing purposes is investigated, and their feasibility is tested by using results obtained during field campaigns of the airborne POLDER instrument, a radiometer designed to measure the directionality and polarization of the sunlight scattered by the ground atmosphere system.

Description: Papers focused on land surface, atmospheric, and ocean properties are reported. Specific comments pertaining to polarization, models and inversion, and measurements, are given. Recommendations are: continued research into the application potential of the BRDF (Bidirectional Reflectance Distribution Function) and polarization properties of ground surface and atmospheric targets; three dimensional models, which account for the statistical behavior of remotely sensed data, should be extended and inverted in order to support analysis of data potentially covering rolling terrain such that pixels represent heterogeneous mixtures of surface cover types and project ground footprints with sizes between 10 to 6 km, the ground pixel sizes of planned future sensors; available reflectance models should be further validated by means of multi dimensional (directional, spectral, temporal) field data and existing models should be intercompared in more depth to evaluate their performance and limitations; existing methods for model inversion should be validated in more depth in order to quantify the practical limitations and the expected accuracy of the parameters retrieved and new approaches should be developed based upon apriori knowledge of plant canopy development and spectral BRDF properties; there is a need to establish a protocol of validation and intercomparison of the indices and compositing techniques which have been proposed during these last years.

Description: Traditionally, the remote sensing community has relied totally on spectral knowledge to extract vegetation characteristics. However, there are other knowledge bases (KB's) that can be used to significantly improve the accuracy and robustness of inference techniques. Using AI (artificial intelligence) techniques a KB system (VEG) was developed that integrates input spectral measurements with diverse KB's. These KB's consist of data sets of directional reflectance measurements, knowledge from literature, and knowledge from experts which are combined into an intelligent and efficient system for making vegetation inferences. VEG accepts spectral data of an unknown target as input, determines the best techniques for inferring the desired vegetation characteristic(s), applies the techniques to the target data, and provides a rigorous estimate of the accuracy of the inference. VEG was developed to: infer spectral hemispherical reflectance from any combination of nadir and/or off-nadir view angles; infer percent ground cover from any combination of nadir and/or off-nadir view angles; infer unknown view angle(s) from known view angle(s) (known as view angle extension); and discriminate between user defined vegetation classes using spectral and directional reflectance relationships developed from an automated learning algorithm. The errors for these techniques were generally very good ranging between 2 to 15% (proportional root mean square). The system is designed to aid scientists in developing, testing, and applying new inference techniques using directional reflectance data.

Description: Most earth surfaces, particularly those supporting natural vegetation ecosystems, constitute structurally and spectrally complex surfaces that are distinctly non-Lambertian reflectors. Obtaining meaningful measurements of the directional radiances of landscapes and obtaining estimates of the complete bidirectional reflectance distribution functions of ground targets with complex and variable landscape and radiometric features are challenging tasks. Reasons for the increased interest in directional radiance measurements are presented, and the issues that must be addressed when trying to acquire directional radiances for vegetated land surfaces from different types of remote sensing platforms are discussed. Priority research emphases are suggested, concerning field measurements of directional surface radiances and reflectances for future research. Primarily, emphasis must be given to the acquisition of more complete and directly associated radiometric and biometric parameter data sets that will empower the exploitation of the 'angular dimension' in remote sensing of vegetation through enabling the further development and rigorous validation of state of the art plant canopy models.

Description: Synthetic aperture radar (SAR) images of the Greenland ice sheet collected by an airborne system clearly reveal the four melting facies of this sheet defined 30 years ago from snow stratigraphy studies by glaciologists. In particular, the radar echoes from the percolation facies have radiometric and polarimetric characteristics that are unique among terrestrial surfaces, but that resemble the exotic radar echoes recorded from the icy Galilean satellites. There, the radar signals interact with subsurface, massive ice features created in the cold, dry snow by seasonal melting and refreezing events. The subsurface features act as efficient reflectors of the incident radiation most likely via internal reflections. In the soaked-snow facies, the radar reflectivity is much lower because radar signals are attenuated by the wetter snow before they can interact with subsurface structures. Inversion algorithms to derive geophysical information from the SAR data are developed in both cases to estimate snow wetness in the soaked-snow facies and the mass of ice water retained in the percolation facies.

Description: An operational stratospheric correction scheme used after the Mount Pinatubo (Phillipines) eruption (Jun. 1991) is presented. The stratospheric aerosol distribution is assumed to be only variable with latitude. Each 9 days the latitudinal distribution of the optical thickness is computed by inverting radiances observed in the NOAA AVHRR channel 1 (0.63 micrometers) and channel 2 (0.83 micrometers) over the Pacific Ocean. This radiance data set is used to check the validity of model used for inversion by checking consistency of the optical thickness deduced from each channel as well as optical thickness deduced from different scattering angles. Using the optical thickness profile previously computed and radiative transfer code assuming Lambertian boundary condition, each pixel of channel 1 and 2 are corrected prior to computation of NDVI (Normalized Difference Vegetation Index). Comparison between corrected, non corrected, and years prior to Pinatubo eruption (1989 to 1990) NDVI composite, shows the necessity and the accuracy of the operational correction scheme.

Description: Aspects of aerosol studies and remote sensing are reviewed. Aerosol scatters solar radiation before it reaches the surface and scatters and absorbs it again after it is reflected from the surface and before it reaches the satellite sensor. The effect is spectrally and spatially dependent. Therefore atmospheric aerosol (dust, smoke and air pollution particles) has a significant effect on remote sensing. Correction for the aerosol effect was never achieved on an operational basis though several case studies were demonstrated. Correction can be done in a direct way by deriving the aerosol loading from the image itself and correcting for it using the appropriate radiative transfer model or by an indirect way, by defining remote sensing functions that are less dependent on the aerosol loading. To some degree this was already achieved in global remote sensing of vegetation where a composite of several days of NDVI (Normalized Difference Vegetation Index) measurements, choosing the maximal value, was used instead of a single cloud screened value. The Atmospheric Resistant Vegetation Index (ARVI) introduced recently for the NASA Earth Observing System EOS-MODIS is the most appropriate example of indirect correction, where the index is defined in such a way that the atmospheric effect in the blue spectral channel cancels to a large degree the atmospheric in the red channel in computations of a vegetation index. Atmospheric corrections can also use aerosol climatology and ground based instrumentation.

Description: A session dedicated to high spectral resolution in the solar spectrum, covering topics of calibration, atmospheric correction, geology/pedology, inland water, and vegetation, is reported. The session showed a high degree of diversity in the topics and the approaches used. It was highlighted that high spectral resolution data could provide atmospherically corrected ground level calibrated reflectance values. Important advances were shown in the use of radiative transfer models applied either on water bodies or vegetation. Several studies highlighted the high degree of redundancy contained in high spectral resolution data.

Description: An analytical solution to the perturbative multiple collision series of a fragmenting HZE ion beam has limited usefulness since the first collision term has several hundred contributions, the second collision term has tens of thousands of contributions, and each successive collision term progresses to unwieldy computational proportions. Our previous work has revealed the multiple collision terms in the straight-ahead approximation to be simple products of a spatially dependent factor times a linear energy-dependent factor of limited domain and unit normalization. The properties of these forms allow the development of the nonperturbative summation of the series to all orders assuming energy-independent nuclear cross sections as matrix products of a scaled Green's function described herein. This nonperturbative Green's function with multiple scattering correction factors compares well with experiments using 670 MeV/u neon-20 ion beams in thick water targets.

Description: Evidence now suggests that satellite cells constitute a class of myogenic cells that differ distinctly from other embryonic myoblasts. Satellite cells arise from somites and first appear as a distinct myoblast type well before birth. Satellite cells from different muscles cannot be functionally distinguished from one another and are able to provide nuclei to all fibers without regard to phenotype. Thus, it is difficult to ascribe any significant function to establishing or stabilizing fiber type, even during regeneration. Within a muscle, satellite cells exhibit marked heterogeneity with respect to their proliferative behavior. The satellite cell population on a fiber can be partitioned into those that function as stem cells and those which are readily available for fusion. Recent studies have shown that the cells are not simply spindle shaped, but are very diverse in their morphology and have multiple branches emanating from the poles of the cells. This finding is consistent with other studies indicating that the cells have the capacity for extensive migration within, and perhaps between, muscles. Complexity of cell shape usually reflects increased cytoplasmic volume and organelles including a well developed Golgi, and is usually associated with growing postnatal muscle or muscles undergoing some form of induced adaptive change or repair. The appearance of activated satellite cells suggests some function of the cells in the adaptive process through elaboration and secretion of a product. Significant advances have been made in determining the potential secretion products that satellite cells make. The manner in which satellite cell proliferative and fusion behavior is controlled has also been studied. There seems to be little doubt that cellcell coupling is not how satellite cells and myofibers communicate. Rather satellite cell regulation is through a number of potential growth factors that arise from a number of sources. Critical to the understanding of this form of control is to determine which of the many growth factors that can alter satellite cell behavior in vitro are at work in vivo. Little work has been done to determine what controls are at work after a regeneration response has been initiated. It seems likely that, after injury, growth factors are liberated through proteolytic activity and initiate an activation process whereby cells enter into a proliferative phase. After myofibers are formed, it also seems likely that satellite cell behavior is regulated through diffusible factors arising from the fibers rather than continuous control by circulating factors.(ABSTRACT TRUNCATED AT 400 WORDS).

Description: When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.

Description: We performed morphologic studies on skin from seven patients with ALS and seven control subjects. By light microscopy, the wide spaces that separated collagen bundles reacted strongly with colloidal iron and alcian blue in ALS patients. Electron microscopy revealed markedly increased amorphous material that was positive for ruthenium red in the ground substance. These findings were not present in controls. Quantitative amino acid analysis showed that the amount of total amino acids (nmoles per mg dry weight) was significantly decreased (p 〈 0.01) in ALS patients compared with that of controls, and there was a significant negative correlation between skin amino acid content and duration of illness in ALS patients (r = -0.83, p 〈 0.001). These morphologic findings and biochemical data indicate that the amorphous material, which is markedly increased in ALS skin, includes glycosaminoglycans.

Description: The present study assessed the contributions of feeding changes and unloading to the overall measured effects of 2-wk hindlimb (Tail) suspension on the mouse femora. Feeding changes were addressed by considering the effects of matched feeding among suspended and control mice. The effects of hind limb unloading were considered by comparing suspended mice to mice equipped identically (though not suspended) and matched-fed. The feeding and unloading aspects of suspension appear to cause distinctly differing effects on the stereotypic modeling of the femora. Matched-feeding was accompanied by increased resorption surface in comparison to suspended mice, while unloading led to reduced bone formation at the mid-diaphysis of the femora. Reduced mineral content was observed in the bones of suspended mice when compared to the other mice groups, but without increased resorption surface. Thus, the unloading aspects of the antiorthostatic suspension protocol apparently causes reduced formation and mineralization in the femur.

Description: Dorsal-ventral patterning in the Xenopus egg becomes established midway through the first cell cycle during a 30 degree rotation of the subcortical yolk mass relative to the egg cortex. This 'rotation of symmetrisation' is microtubule dependent, and its direction is thought to be cued by the usually eccentric sperm centrosome. The fact that parthenogenetically activated eggs also undergo a directed rotation, despite the absence of a sperm centrosome, suggests that an endogenous asymmetry in the unfertilised egg supports the directed polymerisation of microtubules in the vegetal cortex, in the way that an eccentric sperm centrosome would in fertilised eggs. Consistent with this idea, we noticed that the maturation spot is usually located an average of more than 15 degrees from the geometric centre of the pigmented animal hemisphere. In parthenogenetically activated eggs, this eccentric maturation spot can be used to predict the direction of rotation. Although in most fertilised eggs the yolk mass rotates toward the sperm entry point (SEP) meridian, occasionally this relationship is perturbed significantly; in such eggs, the maturation spot is never on the same side of the egg as the SEP. In oocytes tilted 90 degrees from upright during maturation in vitro, the maturation spot developed 15 degrees or more from the centre of the pigmented hemisphere, always displaced towards the point on the equator that was up during maturation. This experimentally demonstrated lability is consistent with an off-axis oocyte orientation during oogenesis determining its eccentric maturation spot position, and, in turn, its endogenous rotational bias.

Description: The mitogen phytohemagglutinin (PHA) works well in both human and cynomolgus monkey (Macaca fascicularis) lymphocyte cultures to stimulate T cell proliferation. T cells from rhesus monkeys (Macaca mulatta) are less responsive than human cells, producing few metaphases when thousands are required, e.g. in biological dosimetry studies. We show that staphylococcal enterotoxin A (SEA), one of the most potent mitogens known, at a concentration of 0.5 microgram/ml stimulated peripheral lymphocytes to grow with a mitotic index (MI) averaging 0.13 metaphases/cell in old, irradiated rhesus macaques. This was significantly greater (p 〈 0.001) than that produced by PHA (MI 〈 0.01) in lymphocytes from the same animals. Whole blood was cultured for 96, 120 and 144 h for five irradiated individuals and for two controls. All cells cultured with SEA produced a high MI with a peak response at 120 h whereas the same cultures showed low MI for each PHA stimulated culture.

Description: No abstract available

Description: The quality of volume-localized magnetic resonance spectroscopy is affected by eddy currents caused by gradient switching. Eddy currents can be reduced with improved gradient systems; however, it has been suggested that the distortion due to eddy currents can be compensated for during postprocessing with a single-frequency reference signal. The authors propose modifying current techniques for acquiring the single-frequency reference signal by using relaxation weighting to reduce interference from components that cannot be eliminated by digital filtering alone. Additional sequences with T1 or T2 weighting for reference signal acquisition are shown to have the same eddy current characteristics as the original signal without relaxation weighting. The authors also studied a new eddy current correction method that does not require a single-frequency reference signal. This method uses two free induction decays (FIDs) collected from the same volume with two sequences with opposite gradients. Phase errors caused by eddy currents are opposite in these two FIDs and can be canceled completely by combining the FIDs. These methods were tested in a phantom. Eddy current distortions were corrected, allowing quantitative measurement of structures such as the -CH = CH- component, which is otherwise undetectable.

Description: Quantitative assessment of the heritable somatic effects of ionizing radiation exposures has relied upon the assumption that radiation-induced lesions were 'fixed' in the DNA prior to the first postirradiation mitosis. Lesion conversion was thought to occur during the initial round of DNA replication or as a consequence of error-prone enzymatic processing of lesions. The standard experimental protocols for the assessment of a variety of radiation-induced endpoints (cell death, specific locus mutations, neoplastic transformation and chromosome aberrations) evaluate these various endpoints at a single snapshot in time. In contrast with the aforementioned approaches, some studies have specifically assessed radiation effects as a function of time following exposure. Evidence has accumulated in support of the hypothesis that radiation exposure induces a persistent destabilization of the genome. This instability has been observed as a delayed expression of lethal mutations, as an enhanced rate of accumulation of non-lethal heritable alterations, and as a progressive intraclonal chromosomal heterogeneity. The genetic controls and biochemical mechanisms underlying radiation-induced genomic instability have not yet been delineated. The aim is to integrate the accumulated evidence that suggests that radiation exposure has a persistent effect on the stability of the mammalian genome.

Description: While studies concerning mitogenic factors have been an important area of research for many years, much less is understood about the mechanisms of action of cell surface growth inhibitors. We have purified an 18 kDa cell surface sialoglycopeptide growth inhibitor (CeReS-18) which can reversibly inhibit the proliferation of diverse cell types. The studies discussed in this article show that three mouse keratinocyte cell lines exhibit sixty-fold greater sensitivity than other fibroblasts and epithelial-like cells to CeReS-18-induced growth inhibition. Growth inhibition induced by CeReS-18 treatment is a reversible process, and the three mouse keratinocyte cell lines exhibited either single or multiple cell cycle arrest points, although a predominantly G0/G1 cell cycle arrest point was exhibited in Swiss 3T3 fibroblasts. The sensitivity of the mouse keratinocyte cell lines to CeReS-18-induced growth inhibition was not affected by the degree of tumorigenic progression in the cell lines and was not due to differences in CeReS-18 binding affinity or number of cell surface receptors per cell. However, the sensitivity of both murine fibroblasts and keratinocytes could be altered by changing the extracellular calcium concentration, such that increased extracellular calcium concentrations resulted in decreased sensitivity to CeReS-18-induced proliferation inhibition. Thus the increased sensitivity of the murine keratinocyte cell lines to CeReS-18 could be ascribed to the low calcium concentration used in their propagation. Studies are currently under way investigating the role of calcium in CeReS-18-induced growth arrest. The CeReS-18 may serve as a very useful tool to study negative growth control and the signal transduction events associated with cell cycling.

Description: Space flight represents a form of sensory stimulus rearrangement requiring modification of established terrestrial response patterns through central reinterpretation. Evidence of sensory reinterpretation is manifested as postflight modifications of eye/head coordination, locomotor patterns, postural control strategies, and illusory perceptions of self or surround motion in conjunction with head movements. Under normal preflight conditions, the head is stabilized during locomotion, but immediately postflight reduced head stability, coupled with inappropriate eye/head coordination, results in modifications of gait. Postflight postural control exhibits increased dependence on vision which compensates for inappropriate interpretation of otolith and proprioceptive inputs. Eye movements compensatory for perceived self motion, rather than actual head movements have been observed postflight. Overall, the in-flight adaptive modification of head stabilization strategies, changes in head/eye coordination, illusionary motion, and postural control are maladaptive for a return to the terrestrial environment.

Description: No abstract available

Description: Bedrest studies of normal subjects provide opportunities to understand physiologic responses to supine posture and inactivity. Furthermore, head-down tilt has been a valuable procedure to investigate adaptation to microgravity and development of countermeasures to maintain the health and well-being of humans during space-flight. Recent bedrest experiments at NASA have ranged in duration from a few hours to 17 weeks. Acute studies of 6 degrees head-down tilt indicate that elevation of capillary blood pressure from 28 to 34 mm Hg and increased capillary perfusion in tissues of the head cause facial and intracranial edema. Intracranial pressure increases from 2 to 17 mm Hg going from upright posture to 6 degrees head-down tilt. Microvessels of the head have a low capacity to constrict and diminish local perfusion. Elevation of blood and tissue fluid pressures/flow in the head may also explain the higher headward bone density associated with long-term head-down tilt. These mechanistic studies of head-down tilt, along with a better understanding of the relative stresses involved with upright posture and lower body negative pressure, have facilitated development of suitable physiologic countermeasures to maintain astronaut health during microgravity. Presently no exercise hardware is available to provide a blood pressure gradient from head to feet in space. However, recent studies in our laboratory suggest that treadmill exercise using a graded lower-body compression suit and 100 mmHg lower body negative pressure provides equivalent or greater physiologic stress than similar upright exercise on Earth. Therefore, exercise within a lower body negative pressure chamber may provide a cost-effective and simple countermeasure to maintain the cardiovascular and neuro-musculoskeletal systems of astronauts during long-duration flight.

Description: There are types of deafness and tinnitus in which ruptures or massive changes in the ionic permeability of the membranes lining the endolymphatic space [e.g., of the reticular lamina (RL)] are believed to allow potassium-rich endolymph to deluge the low [K+] perilymphatic fluid (e.g., in the small spaces of Nuel). This would result in a K+ intoxication of sensory and neural structures. Acute attacks of Meniere's disease have been suggested to be an important example for this event. The present study investigated the effects of transiently elevated [K+] due to the addition of artificial endolymph to the basolateral cell surface of outer hair cells (OHC) in replicating endolymph-induced K+ intoxication of the perilymph in the small spaces of Nuel. The influence of K+ intoxication of the basolateral OHC cell surface on the transduction was then examined. Intoxication resulted in an inhibition of the physiological repolarizing K+ efflux from hair cells. This induced unwanted depolarizations of the hair cells, interfering with mechanoelectrical transduction. A pathological longitudinal OHC shortening was also found, with subsequent compression of the organ of Corti possibly influencing the micromechanics of the mechanically active OHC. Both micromechanical and electrophysiological alterations are proposed to contribute to endolymph leakage induced attacks of deafness and possibly also to tinnitus. Moreover, repeated or long-lasting K+ intoxications of OHC resulted in a chronic and complete loss of OHC motility. This is suggested to be a pathophysiological basis in some patients with chronic hearing loss resulting from Meniere's syndrome.

Description: No abstract available

Description: We have developed a computer monitor of nuclear DNA in the form of chromatin fibre. The fibres are modeled as a ideal solenoid consisting of twenty helical turns with six nucleosomes per turn. The chromatin model, in combination with are Monte Carlo theory of radiation damage induces by charged particles, based on general features of tack structure and stopping power theory, has been used to evaluate the influence of DNA structure on initial damage. An interesting has emerged from our calculations. Our calculated results predict the existence of strong spatial correlations in damage sites associated with the symmetries in the solenoidal model. We have calculated spectra of short fragments of double stranded DNA produced by multiple double strand breaks induced by both high and low LET radiation. The spectra exhibit peaks at multiples of approximately 85 base pairs (the nucleosome periodicity), and approximately 1000 base pairs (solenoid periodicity). Preliminary experiments to investigate the fragment distributions from irradiated DNA, made by B. Rydberg at Lawrence Berkeley Laboratory, confirm the existence of short DNA fragments and are in substantial agreement with the predictions of our theory.

Description: Citrate is an inhibitor of the crystallization of stone-forming calcium salts. Hypocitraturia, frequently encountered in patients with nephrolithiasis, is therefore an important risk factor for stone formation. Potassium citrate provides physiological and physicochemical correction and inhibits new stone formation, not only in hypocitraturic calcium nephrolithiasis but also in uric acid nephrolithiasis. Inhibition of stone recurrence has now been validated by a randomized trial. Ongoing research has disclosed additional causes of hypocitraturia (sodium excess, low intestinal alkali absorption, but not primary citrate malabsorption). Moreover, new insights on potassium citrate action have been shown, notably that some of absorbed citrate escapes oxidation and contributes to the citraturic response, that ingestion with a meal does not sacrifice physiological or physicochemical action, that orange juice mimics but does not completely duplicate its actions, that potassium citrate may have a beneficial bone-sparing effect, that it may reduce stone fragments following ESWL, and that danger of aluminum toxicity is not great in subjects with functioning kidneys. Finally, the research on potassium citrate has led to two promising products, calcium citrate as an optimum calcium supplement and potassium-magnesium citrate which may be superior to potassium citrate in the management of stone disease.

Description: Major strides in the molecular biology of essential hypertension are currently underway. This has tended to obscure the fact that a number of inherited disorders associated with low blood pressure exist and that these diseases may have milder and underrecognized phenotypes that contribute importantly to blood pressure variation in the general population. This review highlights some of the gene products that, if abnormal, could cause hypotension in some individuals. Diseases due to abnormalities in the catecholamine enzymes are discussed in detail. It is likely that genetic abnormalities with hypotensive phenotypes will be as interesting and diverse as those that give rise to hypertensive disorders.

Description: We recorded the horizontal (yaw), vertical (pitch), and torsional (roll) eye movements of two rhesus monkeys with scleral search coils before and after the COSMOS Biosatellite 2229 Flight. The aim was to determine effects of adaptation to microgravity on the vestibulo-ocular reflex (VOR). The animals flew for 11 days. The first postflight tests were 22 h and 55 h after landing, and testing extended for 11 days after reentry. There were four significant effects of spaceflight on functions related to spatial orientation: (1) Compensatory ocular counterrolling (OCR) was reduced by about 70% for static and dynamic head tilts with regard to gravity. The reduction in OCR persisted in the two animals throughout postflight testing. (2) The gain of the torsional component of the angular VOR (roll VOR) was decreased by 15% and 50% in the two animals over the same period. (3) An up-down asymmetry of nystagmus, present in the two monkeys before flight was reduced after exposure to microgravity. (4) The spatial orientation of velocity storage was shifted in the one monkey that could be tested soon after flight. Before flight, the yaw axis eigenvector of optokinetic afternystagmus was close to gravity when the animal was upright or tilted. After flight, the yaw orientation vector was shifted toward the body yaw axis. By 7 days after recovery, it had reverted to a gravitational orientation. We postulate that spaceflight causes changes in the vestibular system which reflect adaptation of spatial orientation from a gravitational to a body frame of reference. These changes are likely to play a role in the postural, locomotor, and gaze instability demonstrated on reentry after spaceflight.

Description: The high molecular weight aggregate (HMWA) fraction was isolated from the water soluble proteins of aged bovine lenses. Its composition and ability to inhibit heat-induced denaturation and aggregation were compared with the lower molecular weight, oligomeric fraction of alpha isolated from the same lens. Although the major components of both fractions were the alpha-A and alpha-B chains, the HMWA fraction possessed a decreased ability to protect other proteins against heat-induced denaturation and aggregation. Immunoelectron microscopy of both fractions demonstrated that alpha particles from the HMWA fraction contained increased amounts of beta and gamma crystallins, bound to a central region of the supramolecular complex. Together, these results demonstrate that alpha crystallins found in the HMWA fraction possess a decreased ability to protect against heat-induced denaturation and aggregation, and suggest that at least part of this decrease could be due to the increased presence of beta and gamma crystallins complexed to the putative chaperone receptor site of the alpha particles.

Description: No abstract available

Description: Experiments conducted on STS-50 indicated that space flight significantly inhibited tumor necrosis factor (TNF)-mediated killing of LM929 cells compared to ground controls. In ground-based studies, activation of protein kinase C (PKC) with phorbol 12-myristate 13-acetate (PMA) also inhibited TNF-mediated killing of LM929 cells. Therefore, we used PKC inhibitors to determine if the inhibitory effects of spaceflight on TNF-mediated cytotoxicity involved the activation of PKC. In experiments conducted onboard space shuttle mission STS-54, we saw that in the presence of the protein kinase C inhibitors H7 and H8, TNF-mediated cytotoxicity was restored to levels of those observed in the ground controls. Subsequent experiments done during the STS-57 mission tested the dose response of two protein kinase inhibitors, H7 and HA1004. We again saw that killing was restored in a dose-dependent manner, with inhibitor concentrations known to inhibit PKC being most effective. These data suggest that space flight ameliorates the action of TNF by affecting PKC in target cells.

Description: Focal sarcomere disruptions were previously observed in adductor longus muscles of rats flown approximately two weeks aboard the Cosmos 1887 and 2044 biosatellite flights. These lesions, characterized by breakage and loss of myofilaments and Z-line streaming, resembled damage induced by unaccustomed exercise that includes eccentric contractions in which muscles lengthen as they develop tension. We hypothesized that sarcomere lesions in atrophied muscles of space flow rats were not produced in microgravity by muscle unloading but resulted from muscle reloading upon re-exposure to terrestrial gravity. To test this hypothesis, we examined temporal changes in sarcomere integrity of adductor longus muscles from rats subjected to 12.5 days of hindlimb suspension unloading and subsequent reloading by return to vivarium cages for 0, 6, 12, or 48 hours of normal weightbearing. Our ultrastructural observations suggested that muscle unloading (0 h reloading) induced myofibril misalignment associated with myofiber atrophy. Muscle reloading for 6 hours induced focal sarcomere lesions in which cross striations were abnormally widened. Such lesions were electron lucent due to extensive myofilament loss. Lesions in reloaded muscles showed rapid restructuring. By 12 hours of reloading, lesions were moderately stained foci and by 48 hours darkly stained foci in which the pattern of cross striations was indistinct at the light and electron microscopic levels. These lesions were spanned by Z-line-like electron dense filamentous material. Our findings suggest a new role for Z-line streaming in lesion restructuring: rather than an antecedent to damage, this type of Z-line streaming may be indicative of rapid, early sarcomere repair.

Description: We assessed the reactivity of purified S-100 antiserum in immuno-electron microscopy by counting the number of gold particles per microns 2 over inner ear tissues embedded in different media. Sections containing predominantly Schwann's cell cytoplasm and nucleus, afferent fiber axoplasm and myelin sheath of chick cochleae were reacted with anti-S-100 IgG, an antibody to a calcium binding protein of neuronal tissues, then labeled with anti-IgG-gold conjugate. This investigation was conducted because previously published procedures, unmodified, did not yield acceptable results. Preparation of all specimens was identical. Only the medium (PolyBed 812, Araldite or Spurr epoxies; and LR White, LR Gold or Lowicryl plastics) was changed. The medium was made the changing variable because antigens available in post-embedding immuno-electron microscopy are decreased by heat, either used and/or released during polymerization of the embedding medium. The results indicate that: (a) none of the embedding media above provided optimal signal-to-noise ratio for all parts of the nerve stained in the same section; (b) aggregation of gold particles over cells was highest in embedding media with high background labeling over areas devoid of tissue (noise); (c) aggregation occurred randomly throughout both cellular and acellular regions; and (d) particles aggregated less and were distributed more evenly in tissues from media yielding good ultrastructural integrity.

Description: A theoretical model is developed to predict the fluid shear stress and streaming potential at the surface of osteocytic processes in the lacunar-canalicular porosity of an osteon when the osteon is subject to mechanical loads that are parallel or perpendicular to its axis. The theory developed in Weinbaum et al. (31) for the flow through a proteoglycan matrix in a canaliculus is employed in a poroelastic model for the osteon. Our formulation is a generalization of that of Petrov et al. (17). Our model predicts that, in order to satisfy the measured frequency dependence of the phase and magnitude of the SGP in macroscopic bone samples, the fiber spacing in the fluid annulus must lie in the narrow range 6-7 nm typical of the spacing of GAG sidechains along a protein monomer. The model predictions for the local SGP profiles in the osteon agree with the experimental observations of Starkebaum et al. (24). The theory predicts that the pore pressure relaxation time, tau d, for a 150-300 microns diameter osteon with the foregoing matrix structure is approximately 0.03-0.13 sec, and that the amplitude of the mean fluid shear stress on the membrane of the osteocytic process at the mean areal radius of the osteon has a maximum at 28 Hz if tau d = 0.06 sec. This maximum, which is independent of the magnitude of the loading, could be important in vivo since the recent experiments of Turner et al. (28) and McLeod et al. (15) have a peak in the strain frequency spectrum between 20 and 30 Hz that also appears to be independent of the type (magnitude) of loading. Numerical predictions for the amplitude of the average fluid shear stress on the osteocytic membrane at the mean areal radius of the osteon show that the fluid shear stress associated with the low amplitude 20-30 Hz spectral strain component is at least as large as the average fluid shear stress associated with the high amplitude 1 Hz stride component, although the latter loading is an order of magnitude larger, and has a magnitude that lies within the middle of the range, 6-30 dynes/cm2, where fluid shear stresses in tissue culture studies with osteoblast monolayers have elicited an intracellular Ca++ response (31). The implications of these results for intracellular electrical communication are discussed.

Description: Class I and class II major histocompatibility complex (MHC) molecules play significant roles in T cell development and immune function. We show that MHCI- and MHCII-deficient mice have low numbers of macrophage precursors and circulating monocytes, as well as abnormal bone marrow cell colony-stimulating factor type 1 secretion and bone composition. We suggest that MHCI and MHCII molecules play a significant role in macrophage development.

Description: Recently, it was observed that noncoding regions of DNA sequences possess long-range power-law correlations, whereas coding regions typically display only short-range correlations. We develop an algorithm based on this finding that enables investigators to perform a statistical analysis on long DNA sequences to locate possible coding regions. The algorithm is particularly successful in predicting the location of lengthy coding regions. For example, for the complete genome of yeast chromosome III (315,344 nucleotides), at least 82% of the predictions correspond to putative coding regions; the algorithm correctly identified all coding regions larger than 3000 nucleotides, 92% of coding regions between 2000 and 3000 nucleotides long, and 79% of coding regions between 1000 and 2000 nucleotides. The predictive ability of this new algorithm supports the claim that there is a fundamental difference in the correlation property between coding and noncoding sequences. This algorithm, which is not species-dependent, can be implemented with other techniques for rapidly and accurately locating relatively long coding regions in genomic sequences.

Description: Organ culture of embryonic mouse lung and pancreas rudiments has been used to investigate development and differentiation, and to assess the effects of microgravity on culture differentiation, during orbital spaceflight of the shuttle Endeavour (mission STS-54). Lung rudiments continue to grow and branch during spaceflight, an initial result that should allow future detailed study of lung morphogenesis in microgravity. Cultured embryonic pancreas undergoes characteristic exocrine acinar tissue and endocrine islet tissue differentiation during spaceflight, and in ground controls. The rudiments developing in the microgravity environment of spaceflight appear to grow larger than their ground counterparts, and they may have differentiated more rapidly than controls, as judged by exocrine zymogen granule presence.

Description: No abstract available

Description: Myoblast cell cultures have been widely employed in conventional (1g) studies of biological processes because characteristics of intact muscle can be readily observed in these cultured cells. We decided to investigate the effects of spaceflight on muscle by utilizing a well characterized myoblast cell line (L8 rat myoblasts) as cultured in the recently designed Space Tissue Loss Flight Module "A" (STL-A). The STL-A is a "state of the art," compact, fully contained, automated cell culture apparatus which replaces a single mid-deck locker on the Space Shuttle. The L8 cells were successfully flown in the STL-A on the Space Shuttle STS-45 mission. Upon return to earth, reculturing of these spaceflown L8 cells (L8SF) resulted in their unexpected failure to fuse and differentiate into myotubes. This inability of the L8SF cells to fuse was found to be a permanent phenotypic alteration. Scanning electron microscopic examination of L8SF cells growing at 1g on fibronectin-coated polypropylene fibers exhibited a strikingly different morphology as compared to control cells. In addition to their failure to fuse into myotubes, L8SF cells also piled up on top of each other. When assayed in fusion-promoting soft agar, L8SF cells gave rise to substantially more and larger colonies than did either preflight (L8AT) or ground control (L8GC) cells. All data to this point indicate that flying L8 rat myoblasts on the Space Shuttle for a duration of 7-10 d at subconfluent densities results in several permanent phenotypic alterations in these cells.

Description: Insulin-like growth factors (IGF) are important regulators of skeletal growth. To determine whether the capacity to produce and respond to these growth factors changes during skeletal development, we measured the protein and mRNA levels for IGF-I, IGF-II, and their receptors (IGF-IR and IGF-IIR, respectively) in the tibia and femur of rats before and up to 28 mo after birth. The mRNA levels remained high during fetal development but fell after birth, reaching a nadir by 3-6 wk. This fall was most pronounced for IGF-II and IGF-IIR mRNA and least pronounced for IGF-I mRNA. However, after 6 wk, both IGF-I and IGF-IR mRNA levels recovered toward the levels observed at birth. In the prenatal bones, the signals for the mRNAs of IGF-II and IGF-IIR were stronger than the signals for the mRNAs of IGF-I and IGF-IR, although the content of IGF-I was three- to fivefold greater than that of IGF-II. IGF-II levels fell postnatally, whereas the IGF-I content rose after birth such that the ratio IGF-I/IGF-II continued to increase with age. We conclude that, during development, rat bone changes its capacity to produce and respond to IGFs with a progressive trend toward the dominance of IGF-I.

Description: In eukaryotic cells, microtubules are 24-nm-diameter tubular structures composed of a class of conserved proteins called tubulin. They are involved in numerous cell functions including ciliary motility, nerve cell elongation, pigment migration, centrosome formation, and chromosome movement. Although cytoplasmic tubules and fibers have been observed in bacteria, some with diameters similar to those of eukaryotes, no homologies to eukaryotic microtubules have been established. Certain groups of bacteria including azotobacters, cyanobacteria, enteric bacteria, and spirochetes have been frequently observed to possess microtubule-like structures, and others, including archaebacteria, have been shown to be sensitive to drugs that inhibit the polymerization of microtubules. Although little biochemical or molecular biological information is available, the differences observed among these prokaryotic structures suggest that their composition generally differs among themselves as well as from that of eukaryotes. We review the distribution of cytoplasmic tubules in prokaryotes, even though, in all cases, their functions remain unknown. At least some tend to occur in cells that are large, elongate, and motile, suggesting that they may be involved in cytoskeletal functions, intracellular motility, or transport activities comparable to those performed by eukaryotic microtubules. In Escherichia coli, the FtsZ protein is associated with the formation of a ring in the division zone between the newly forming offspring cells. Like tubulin, FtsZ is a GTPase and shares with tubulin a 7-amino-acid motif, making it a promising candidate in which to seek the origin of tubulins.

Description: Current therapy for massive venous air embolism (VAE) includes the use of the left lateral recumbent (LLR) position. This recommendation is based on animal studies, conducted 50 yr ago, which looked primarily at survival. Little is known, however, about the concomitant hemodynamic response after VAE in various body positions. The purpose of this study was to investigate the hemodynamic and cardiovascular changes in various body positions after VAE. Twenty-two mechanically ventilated supine mongrel dogs received a venous air infusion of 2.5 mL/kg at a rate of 5 mL/s. One minute after the infusion, 100% oxygen ventilation was commenced and the body position of the dogs was changed to either the LLR (n = 6), the LLR with the head 10 degrees down (LLR-10 degrees; n = 6) or the right lateral recumbent (RLR; n = 5) position. Five dogs were maintained in the supine position (SUP; n = 5). One dog died in every group except in the SUP group, where all the dogs recovered. There were no significant differences among the various body positions in terms of heart rate, mean arterial pressure, pulmonary artery pressure, central venous pressure, left ventricular end-diastolic pressure, or cardiac output. The acute hemodynamic changes occurring during the first 5-15 min after VAE recovered to 80% of control within 60 min. Our data suggest that body repositioning does not influence the cardiovascular response to VAE. Specifically, our data do not support the recommendation of repositioning into the LLR position for the treatment of VAE.

Description: No abstract available

Description: This paper reviews a series of studies that indicate that estrogens play an important role in blood volume regulation. The first study illustrates that the plasma volume (PV) of ambulatory women fluctuates during the menstrual cycle, increasing during periods of elevated estrogens. In the second study, it was shown that exogenous and endogenous elevations in blood estrogens attenuate the decrease in PV during bed rest. In the third study, the hypothesis was tested that women, who naturally have a higher blood estrogen content compared with men, will have a smaller loss of PV during bed rest. Ten men and ten women underwent a 13-day, 6 degrees head-down bed rest. Plasma volume and red cell mass (RCM) were measured before and after bed rest using 125I and 51Cr labeling, respectively. Before bed rest, the men and women had similar blood volume (BV) and PV (mL/kg body weight), but the women had a smaller (P 〈 .01) RCM (22.2 +/- 0.9 versus 26.2 +/- 0.8 mL/kg, mean +/- SE). During bed rest, the decrease in RCM (mL/kg) was similar in men and women. However, the decrease in BV was greater in men (8.0 +/- 0.8 mL/kg versus 5.8 +/- 0.8 mL/kg), because of a greater reduction in PV (6.3 +/- 0.6 mL/kg versus 4.1 +/- 0.6 mL/kg). Because the decline in BV has been proposed to contribute to the cardiovascular deconditioning after bed rest, it is possible that women may experience less cardiac and circulatory strain on reambulation.

Description: Life sciences activities being planned for Space Station Freedom (SSF) as of Fall 1992 are discussed. Planning for these activities is ongoing. Therefore, this description should be viewed as indicative of the prevailing ideas at one particular time in the SSF development cycle. The proposed contributions of the Canadian Space Agency (CSN) the European Space Agency (ESA), Japan, and the United States are all discussed in detail. In each case, the life sciences goals, and the way in which each partner proposes to achieve their goals, are reviewed.

Description: This paper reviews the cardiovascular responses of six healthy male subjects to 6 hours in a 5 degrees head-down bed rest model of weightlessness, and compares these responses to those obtained when subjects were positioned in head-up tilts of 10 degrees, 20 degrees, and 42 degrees, simulating 1/6, 1/3, and 2/3 G, respectively. Thoracic fluid index, cardiac output, stroke volume, and peak flow were measured using impedance cardiography. Cardiac dimensions and volumes were determined from two-dimensional guided M-mode echocardiograms in the left lateral decubitus position at 0, 2, 4, and 6 hours. Cardiovascular response to a stand test were compared before and after bed rest. The impedance values were related to tilt angle for the first 2 hours of tilt; however, after 3 hours, at all four angles, values began to converge, indicating that cardiovascular homeostatic mechanisms seek a common adapted state, regardless of effective gravity level (tilt angle) up to 2/3 G. Echocardiography revealed that left ventricular end-diastolic and end-systolic volume, stroke volume, ejection fraction, heart rate, and cardiac output had returned to control values by hour 6 for all tilt angles. The lack of a significant immediate change in left ventricular end-diastolic volume, despite decrements in stroke volume (P 〈 .05) and heart rate (not significant), indicates that multiple factors may play a role in the adaptation to simulated hypogravity. The echocardiography data indicated that no angle of tilt, whether head-down or head-up for 4 to 6 hours, mimicked exactly the changes in cardiovascular function recorded after 4 to 6 hours of space flight. Changes in left ventricular end-diastolic volume during space flight and tilt may be similar, but follow a different time course. Nevertheless, head-down tilt at 5 degrees for 6 hours mimics some (stroke volume, systolic and diastolic blood pressure, mean arterial blood pressure, and total resistance), but not all, of the changes occurring in an equivalent time of space flight. The magnitude of the change in the mean heart rate response to standing was greater after six hours of tilt at -5 degrees or 10 degrees. Thus, results from the stand test after 6 hours of bed rest at -5 degrees and 10 degrees, but not at 20 degrees or 42 degrees, are similar to those obtained after space flight.

Description: No abstract available

Description: Microbalanced stimuli are dynamic displays which do not stimulate motion mechanisms that apply standard (Fourier-energy or autocorrelational) motion analysis directly to the visual signal. In order to extract motion information from microbalanced stimuli, Chubb and Sperling [(1988) Journal of the Optical Society of America, 5, 1986-2006] proposed that the human visual system performs a rectifying transformation on the visual signal prior to standard motion analysis. The current research employs two novel types of microbalanced stimuli: half-wave stimuli preserve motion information following half-wave rectification (with a threshold) but lose motion information following full-wave rectification; full-wave stimuli preserve motion information following full-wave rectification but lose motion information following half-wave rectification. Additionally, Fourier stimuli, ordinary square-wave gratings, were used to stimulate standard motion mechanisms. Psychometric functions (direction discrimination vs stimulus contrast) were obtained for each type of stimulus when presented alone, and when masked by each of the other stimuli (presented as moving masks and also as nonmoving, counterphase-flickering masks). RESULTS: given sufficient contrast, all three types of stimulus convey motion. However, only one-third of the population can perceive the motion of the half-wave stimulus. Observers are able to process the motion information contained in the Fourier stimulus slightly more efficiently than the information in the full-wave stimulus but are much less efficient in processing half-wave motion information. Moving masks are more effective than counterphase masks at hampering direction discrimination, indicating that some of the masking effect is interference between motion mechanisms, and some occurs at earlier stages. When either full-wave and Fourier or half-wave and Fourier gratings are presented simultaneously, there is a wide range of relative contrasts within which the motion directions of both gratings are easily determinable. Conversely, when half-wave and full-wave gratings are combined, the direction of only one of these gratings can be determined with high accuracy. CONCLUSIONS: the results indicate that three motion computations are carried out, any two in parallel: one standard ("first order") and two non-Fourier ("second-order") computations that employ full-wave and half-wave rectification.

Description: The 3-dimensional crystal structure of glutathione S-transferase (GST) of Schistosoma japonicum (Sj) fused with a conserved neutralizing epitope on gp41 (glycoprotein, 41 kDa) of human immunodeficiency virus type 1 (HIV-1) (Muster T et al., 1993, J Virol 67:6642-6647) was determined at 2.5 A resolution. The structure of the 3-3 isozyme rat GST of the mu gene class (Ji X, Zhang P, Armstrong RN, Gilliland GL, 1992, Biochemistry 31:10169-10184) was used as a molecular replacement model. The structure consists of a 4-stranded beta-sheet and 3 alpha-helices in domain 1 and 5 alpha-helices in domain 2. The space group of the Sj GST crystal is P4(3)2(1)2, with unit cell dimensions of a = b = 94.7 A, and c = 58.1 A. The crystal has 1 GST monomer per asymmetric unit, and 2 monomers that form an active dimer are related by crystallographic 2-fold symmetry. In the binding site, the ordered structure of reduced glutathione is observed. The gp41 peptide (Glu-Leu-Asp-Lys-Trp-Ala) fused to the C-terminus of Sj GST forms a loop stabilized by symmetry-related GSTs. The Sj GST structure is compared with previously determined GST structures of mammalian gene classes mu, alpha, and pi. Conserved amino acid residues among the 4 GSTs that are important for hydrophobic and hydrophilic interactions for dimer association and glutathione binding are discussed.

Description: Allan Hills (ALH) 84001 is the most recently recognized member of a suite of meteorites--the SNCs--that almost certainly originated on Mars. Several factors distinguish ALH84001 from the other SNC meteorites. Preliminary studies suggest that it may be older than other martian meteorites. Moreover, it contains abundant, zoned domains of calcium-iron-magnesium carbonate that are indigenous to the sample and thus may hold important clues regarding near-surface processes on Mars and the evolution of the martian atmosphere. We report here analyses of the carbon and oxygen stable-isotope compositions of the carbonates that place constraints on their formation conditions. Our results imply the presence of at least two chemically distinct carbonates--one Ca,Fe-rich, the other Mg-rich--that are enriched in 13C relative to terrestrial carbonates (delta 13C approximately +41/1000), consistent with martian atmospheric CO2 as the carbon source. The oxygen isotope compositions of the carbonates indicate that they precipitated from a low-temperature fluid in the martian crust. Combined with textural and bulk geochemical considerations, the isotope data suggest that carbonate deposition took place in an open-system environment in which the ambient temperature fluctuated.

Description: A repair/misrepair kinetic model for multiple radiation-induced lesions (mutation inactivation) is coupled to a two-mutation model of initiation-promotion in tissue to provide a parametric description of tumour prevalence in the mouse Harderian gland from high-energy and charge radiations. Track-structure effects are considered using an action-cross section model. Dose-response curves are described for gamma rays and relativistic ions, and good agreement with experiment is found. The effects of nuclear fragmentation are also considered for high-energy proton and alpha-particle exposures. The model described provides a parametric description of age-dependent cancer induction for a wide range of radiation fields. Radiosensitivity parameters found in the model for an initiation mutation (sigma 0 = 7.6 x 10(-10) cm2 and D0 = 148.0 Gy) are somewhat different than previously observed for neoplastic transformation of C3H10T1/2 cell cultures (sigma 0 = 0.7 x 10(-10) cm2 and D0 = 117.0 Gy). We consider the two hypotheses that radiation acts solely as an initiator or as both initiator and promoter and make model calculations for fractionation exposures from gamma rays and relativistic Fe ions. For fractionated Fe exposures, an inverse-dose-rate effect is provided by a promotion hypothesis with an increase of 30% or more, dependent on the dose level and fractionation schedule, using a mutation rate for promotion similar to that of single-gene mutations.

Description: The production of protons in heavy ion collisions through the knockout mechanism (abrasion) is described using the Glauber model. The multiple knockouts from the projectile, including the inelastic collision series with the target, are considered using a closure approximation in treating energy conservation. Calculations for reactions of 12C projectiles with several targets at energies of 1 and 2A GeV arc compared to experiments. For large secondary proton momentum a strong dependence on the target mass is found and attributed to multiple scattering of the projectile knockouts.

Description: The Galileo Ultravilet Spectrometer Experiment (UVS) obtained a partial celestial sphere map of interplanetary Lyman-alpha (IP L alpha) on 13-14 December 1990 during the first Earth encounter. The Galileo spacecraft was near the downwind axis of the local interstellar medium flow. These UVS measurements sampled the downwind, anti-sunward hemisphere. The data were modeled using a hot model of the interplanetary hydrogen density distribution with the goal of studying multiple scattering effects in the inner solar system. The derived ratio in the downwind direction of the observed brightness and a single scattering model brightness, both normalized to unity in the upwind direction, is 1.82 +/- 0.2. This brightness ratio requires a multiple scattering correction which is 36% larger than can be accounted for by theoretical calculations. The hot model may require: (1) a temperature perturbation of the interstellar wind velocity distribution or (2) an additional downstream source of interplanetary hydrogen. However, a more likely exlanation which affects the hot model is the latitude dependence of the radiation pressure. This dependence, based on the known solar L alpha flux latitude variation at solar maximum, causes a downwind brightness enhancement by preferential focusing of H-atoms with trajectory planes containing the solar poles. This result implies that radiation pressure near the solar poles is nearly independent of solar cycle and is insufficient to lead to a net repulsion of hydrogen atoms by the sun, as can occur near the ecliptic plane during the solar maximum. In addition, the UVS performed 13 observations of IP L alpha while in cruise between Venus and the Earth in 3 directions fixed in ecliptic coordinates.

Description: In this paper we generalize earlier gasdynamic analyses of the motion of the heliospheric termination shock in response to upstream disturbances (Barnes, 1993, 1994; Naidu and Barnes, 1994), to include magnetohydrodynamic (MHD) phenomena. We assume that the termination shock is a strong, perpendicular shock and that the initial upstream disturbance is a tangential discontinuity. The resulting configuration after the interaction is very similar to that in the gasdynamic models after an interaction with a contact discontinuity or interplanetary shock, and for an increase (decrease) in dynamic pressure consists of an outward (inward) propagating termination shock and an outward propagating shock (MHD rarefraction wave) that carries the signal of the disturbance into the far downstream plasma. The plasma immediately behind the new termination shock is separated from the downstream signal by a tangential discontinuity. The results of the model show that the speed of the new termination shock depends mainly on the magnitude of the change in dynamic pressure and are typically of order approximately 100 km/s, comparable to the results of the gasdynamic models.

Description: The compressible dynamic stall flowfield over a NACA 0012 airfoil transiently pitching from 0 to 60 deg at a constant rate under compressible flow conditions has been studied using real-time interferometry. A quantitative description of the overall flowfield, including the finer details of dynamic stall vortex formation, growth, and the concomitant changes in the airfoil pressure distribution, has been provided by analyzing the interferograms. For Mach numbers above 0.4, small multiple shocks appear near the leading edge and are present through the initial stages of dynamic stall. Dynamic stall was found to occur coincidentally with the bursting of the separation bubble over the airfoil. Compressibility was found to confine the dynamic stall vortical structure closer to the airfoil surface. The measurements show that the peak suction pressure coefficient drops with increasing freestream Mach number, and also it lags the steady flow values at any given angle of attack. As the dynamic stall vortex is shed, an anti-clockwise vortex is induced near the trailing edge, which actively interacts with the post-stall flow.

Description: The effect of the porous leading edge of an airfoil on the blade-vortex interaction noise, which dominates the far-field acoustic spectrum of the helicopter, is investigated. The thin-layer Navier-Stokes equations are solved with a high-order upwind-biased scheme and a multizonal grid system. The Baldwin-Lomax turbulence model is modified for considering transpiration on the surface. The amplitudes of the propagating acoustic wave in the near field are calculated directly from the computation. The porosity effect on the surface is modeled in two ways: (1) imposition of prescribed transpiration velocity distribution and (2) calculation of transpiration velocity distribution by Darcy's law. Results show leading-edge transpiration can suppress pressure fluctuations at the leading edge during blade-vortex interaction and consequently reduce the amplitude of propagating noise by 30% at a maximum in the near field.

Description: A method has been developed for calculating the viscous flow about airfoils with and without deflected flaps at -90 deg incidence. This method provides for the solution of the unsteady incompressible Navier-Stokes equations by means of an implicit technique. The solution is calculated on a body-fitted computational mesh using a staggered-grid method. The vorticity is defined at the node points, and the velocity components are defined at the mesh-cell sides. The staggered-grid orientation provides for accurate representation of vorticity at the node points and the continuity equation at the mesh-cell centers. The method provides for the noniterative solution of the flowfield and satisfies the continuity equation to machine zero at each time step. The method is evaluated in terms of its stability to predict two-dimensional flow about an airfoil at -90-deg incidence for varying Reynolds number and laminar/turbulent models. The variations of the average loading and surface pressure distribution due to flap deflection, Reynolds number, and laminar or turbulent flow are presented and compared with experimental results. The comparisom indicate that the calculated drag and drag reduction caused by flap deflection and the calculated average surface pressure are in excellent agreement with the measured results at a similar Reynolds number.

Description: Spectral absorption-coefficients (cross-sections) kappa(sub nu) (/cm/atm) have been measured in the 7.62, 8.97, and 12.3 micrometer bands of HCFC-22 (CHClF2) and the 10.6 micrometer bands of SF6 employing a high-resolution Fourier-transform spectrometer. Temperature and total pressure have been varied to simulate conditions corresponding to tropospheric and stratospheric layers in the atmosphere. The kappa(sub nu) are compared with values measured by us previously using a tunable diode laser spectrometer and with the appropriate entries in HITRAN and GEISA, two of the databases known to the atmospheric scientist. The measured absolute intensities of the bands are compared with previously published values.

Description: The term 'magnetic hole' has been used to denote isolated intervals when the magnitude of the interplanetary magnetic field drops to a few tenths, or less, of its ambient value for a time that corresponds to a linear dimension of tens to a few hundreds of proton gyro-radii. Data obtained by the Ulysses magnetometer and solar wind anlayzer have been combined to study the properties of such magnetic holes in the solar wind between 1 AU and 5.4 AU and to 23 deg south latitude. In order to avoid confusion with decreases in field strength at interplanetary discontinuities, the study has focused on linear holes across which the field direction changed by less than 5 deg. The holes occurred preferentially, but not without exception, in the interaction regions on the leading edges of high-speed solar wind streams. Although the plasma surrounding the holes was generally stable against the mirror instability, there are indications that the holes may have been remnants of mirror-mode structures created upstream of the points of observation. Those indications include the following: (1) For the few holes for which proton of alpha-particle pressure could be measured inside the hole, the ion thermal pressure was always greater than in the plasma adjacent to the holes. (2) The plasma surrounding many of the holes was marginally stable for the mirror mode, while the plasma environment of all holes was significantly closer to mirror instability than was the average solar wind. (3) The plasma containing trains of closely spaced holes was closer to mirror instability than was the plasma containing isolated holes. (4) The near-hole plasma had much higher ion beta (ratio of thermal to magnetic pressure) than did the average solar wind. (5) Near the holes, T(sub perp)/T(sub parallel) tended to be either greater than 1 or larger than in the average wind. (6) The proton and alpha-particle distribution functions measured inside the holes occasionally exhibited the flattened phase-space-density contoures in nu(sub perp)/nu(sub parallel) space found in some numerical simulations of the mirror instability.

Description: Rotor noise prediction codes predict the thickness and loading noise produced by a helicopter rotor, given the blade motion, rotor operating conditions, and fluctuating force distribution over the blade surface. However, the criticality of these various inputs, and their respective effects on the predicted acoustic field, have never been fully addressed. This paper examines the importance of these inputs, and the sensitivity of the acoustic predicitions to a variation of each parameter. The effects of collective and cyclic pitch, as well as coning and cyclic flapping, are presented. Blade loading inputs are examined to determine the necessary spatial and temporal resolution, as well as the importance of the chordwise distribution. The acoustic predictions show regions in the acoustic field where significant errors occur when simplified blade motions or blade loadings are used. An assessment of the variation in the predicted acoustic field is balanced by a consideration of Central Processing Unit (CPU) time necessary for the various approximations.

Description: The U.S. National Aeronautics and Space Administration (NASA) Balloon Program has been highly successful since recovering from the catastrophic balloon failure problems of the early to mid 1980s. Balloons have continued to perform at unprecedented success rates. The comprehensive research and development (R&D) effort has continued with advances being made across the spectrum of balloon related disciplines. The long duration balloon project will be transitioning from a development effort to an operational capability this year. Recently, emphasis has been placed on the development and implementation of new support systems and facilities. A new permanent launch facility at Fort Sumner, New Mexico has been established. New ground station support equipment is being implemented, and a new heavy load launch vehicle is scheduled to be implemented in 1992. The progress, status and future plans for these and other aspects of the NASA program will be presented.

Description: The catastrophic balloon failure during the first half of the 1980's identified the need for a comprehensive and continuing balloon research and development (R&D) commitment by NASA. Technical understanding was lacking in many of the disciplines and processes associated with scientific ballooning. A comprehensive balloon R&D plan was developed in 1986 and implemented in 1987. The objectives were to develop the understanding of balloon system performance, limitations, and failure mechanisms. The program consisted of five major technical areas: structures, performance and analysis, materials, chemistry and processing, and quality control. Research activitites have been conducted at NASA/Goddard Space Flight Center (GSFC)-Wallops Flight Facility (WFF), other NASA centers and government facilities, universities, and the balloon manufacturers. Several new and increased capabilities and resources have resulted from this activity. The findings, capabilities, and plan of the balloon R&D program are presented.

Description: Caps have been used to structurally reinforce scientific research balloons since the late 1950's. The scientific research balloons used by the National Aeronautics and Space Administration (NASA) use internal caps. A NASA cap placement specification does not exist since no empirical information exisits concerning cap placement. To develop a cap placement specification, NASA has completed two in-hangar inflation tests comparing the structural contributions of internal caps and external caps. The tests used small scale test balloons designed to develop the highest possible stresses within the constraints of the hangar and balloon materials. An externally capped test balloon and an internally capped test balloon were designed, built, inflated and simulated to determine the structural contributions and benefits of each. The results of the tests and simulations are presented.

Description: During the international campaign of June 1991, the active region AR 6659 produced six very large, long-duration flares (X10/12) during its passage across the solar disk. We present the characteristics of four of them (June 4, 6, 9, 15). Precise measurements of the spot motions from Debrecen and Tokyo white-light pictures are used to understand the fragmentation of the main sunspot group with time. This fragmentation leads to a continuous restructuring of the magnetic field pattern while rapid changes are evidenced due to fast new flux emergence (magnetograms of Marshall Space Flight Center (MSFC), Huairou). The first process leads to a shearing of the field lines along which there is energy storage; the second one is the trigger which causes the release of energy by creating a complex topology. We conjecture that these two processes with different time scales are relevant to the production of flares.

Description: The purpose of this Note is to present results from an analytic/experimental study that investigated the potential for passively changing blade twist through the use of extension-twist coupling. A set of composite model rotor blades was manufactured from existing blade molds for a low-twist metal helicopter rotor blade, with a view toward establishing a preliminary proof concept for extension-twist-coupled rotor blades. Data were obtained in hover for both a ballasted and unballasted blade configuration in sea-level atmospheric conditions. Test data were compared with results obtained from a geometrically nonlinear analysis of a detailed finite element model of the rotor blade developed in MSC/NASTRAN.

Description: The paper considers the compressible Rayleigh equation as a model for the Mach wave emission mechanism associated with high-temperature supersonic jets. Solutions to the compressible Rayleigh equation reveal the existence of several families of supersonically convecting instability waves. These waves directly radiate noise to the jet far field. The predicted noise characteristics are compared to previously acquired experimental data for an axisymmetric Mach 2 fully pressure balanced jet operating over a range of jet total temperatures from ambient to 1370 K. The results of this comparison show that the first-order supersonic instability wave and the Kelvin-Hemlhlotz first-, second-, and third-order modes have directional radiation characteristics that are in agreement with observed data. The assumption of equal initial amplitudes for all of the waves leads to the conclusion that the flapping mode of instability dominates the noise radiatio process of supersonic jets. At a jet temperature of 1370 K, supersonic instability waves are predicted to dominate the noise radiated at high frequency at narrow angles to the jet axis.

Description: The objective of the present work is to study the mixing characteristics of a linear array of supersonic rectangular jets under conditions of screech synchronization. The screech synchronization at a fully expanded jet Mach number of 1.61 is achieved by a precise adjustment of the internozzle spacing. To our knowledge, such an experiment on the resonant mixing of screech synchronized multiple rectangular jets has not been reported before. The results are compared with the case where the screech was suppressed in the multijet configuration.

Description: The objective of the present investigation is to assess the effect of the spatial order of accuracy used for the evaluation of the inviscid fluxes on the resolution of higher order quantitites, such as velocity gradients. The viscous terms are computed as second-order accurate with central difference formulas, even though for the explicit part of the algorithm higher order approximations may be used. A viscous/inviscid method is used, and the outer part of the flowfield is computed with the inviscid flow equations. The viscous boundary-layer type flow region close to the body surface is computed with an algebraic eddy viscosity model. Results obtained with the conservative and nonconservative formulations and the viscous/inviscid approach are compared with available experimental data. The effect of grid refinement on the accuracy of the solution is also presented.

Description: During the late declining phase of the solar cycle, the tilt of the solar magnetic dipole with respect to the Sun's rotation axis leads to large-scale organization of the solar wind, such that alternating regions of high- and low-speed solar wind are observed in the ecliptic plane. In this paper, we use Doppler scintillation measurements to investigate mass flux of these two types of solar wind in the ecliptic plane and inside 0.3 AU, where in situ measurements have not been possible. To the extent that Doppler scintillation reflects mass flux, we find that mass flux in high-speed streams: (1) is lower (by a factor of approximately 2.2) than the mass flux of the average solar wind in the heliocentric distance range of 0.3-0.5 AU; (2) is lower still (by as much as a factor of about 4) than the mass flux of the slow solar wind associated with the streamer belt; and (3) appears to grow with heliocentric distance. These Doppler scintillation results are consistent with the equator to pole decrease in mass flux observed in earlier spectral broadening measurements, and with trends and differences between high- and low-speed solar wind observed by in situ measurements in the range of 0.3-0.1 AU. The mass flux results suggest that the solar wind flow in high-speed streams is convergent towards the ecliptic near the Sun, becoming less convergent and approaching radial with increasing heliocentric distance beyond 0.3 AU. The variability of mass flux observed within equatorial and polar high-speed streams close to the Sun is strikingly low. This low variability implies that, as Ulysses currently ascends to higher latitudes and spends more time in the south polar high-speed stream after crossing the heliocentric current sheet, it can expect to observe a marked decrease in variations of both mass flux and solar wind speed, a trend that appears to have started already.

Description: Highly structured Langmuir waves, also known as electron plasma oscillations, have been observed in the foreshock of Venus using the plasma wave experiment on the Galileo spacecraft during the gravity assist flyby on February 10, 1990. The Galileo wideband sampling system provides digital electric field waveform measurements at sampling rates up to 201,600 samples per second, much higher than any previous instrument of this type. The main Langmuir wave emission band occurs near the local electron plasma frequency, which was approximately 43 kHz. The Langmuir waves are observed to shift above and below the plasma frequency, sometimes by as much as 20 kHz. The shifts in frequency are closely correlated with the downstream distance from the tangent field line, implying that the shifts are controlled by the electron beam velocity. Considerable fine structure is also evident, with timescales as short as 0.15 ms, corresponding to spatial scales of a few tens of Debye lengths. The frequency spectrum often consists of beat-type waveforms, with beat frequencies ranging from 0.2 to 7 kHz, and in a few cases, isolated wave packets. The peak electric field strengths are approximately 1 mV/m. These field strengths are too small for strongly nonlinear processes to be important. The beat-type waveforms are suggestive of a parametric decay process.

Description: The irregular polygonal pattern of solar granulation is analyzed for size-shape relations using statistical crystallography. In contrast to previous work which has assumed perfectly hexagonal patterns for granulation, more realistic accounting of cell (granule) shapes reveals a broader basis for quantitative analysis. Several features emerge as noteworthy: (1) a linear correlation between number of cell-sides and neighboring shapes (called Aboav-Weaire's law); (2) a linear correlation between both average cell area and perimeter and the number of cell-sides (called Lewis's law and a perimeter law, respectively) and (3) a linear correlation between cell area and squared perimeter (called convolution index). This statistical picture of granulation is consistent with a finding of no correlation in cell shapes beyond nearest neighbors. A comparative calculation between existing model predictions taken from luminosity data and the present analysis shows substantial agreements for cell-size distributions. A model for understanding grain lifetimes is proposed which links convective times to cell shape using crystallographic results.

Description: Microwave radar and radiometer measurements of grasslands indicate a substantial reduction in sensor sensitivity to soil moisture in the presence of a thatch layer. When this layer is wet it masks changes in the underlying soil, making the canopy appear warm in the case of passive sensors (radiometer) and decreasing backscatter in the active case (scatterometer). A model for a grass canopy with thatch will be presented in this paper to explain this behavior and to compare with observations. The canopy model consists of three layers: grass, thatch, and the underlying soil. The grass blades are modeled by elongated elliptical discs and the thatch is modeled as a collection of disk shaped water droplets (i.e., the dry matter is neglected). The ground is homogeneous and flat. The distorted Born approximation is used to compute the radar cross section of this three layer canopy and the emissivity is computed from the radar cross section using the Peake formulation for the passive problem. Results are computed at L-band (1.4 GHz) and C-band (4.75 GHz) using canopy parameters (i.e., plant geometry, soil moisture, plant moisture, etc.) representative of Konza Prairie grasslands. The results are compared to C-band scatterometer measurements and L-band radiometer measurements at these grasslands.

Description: Dual-frequency ranging and Doppler measurements were conducted in support of the Ulysses Solar Corona Experiment (SCE) at and around the spacecraft's first solar conjunction in 1991 August. The differential group delay time between range codes on the two downlink carrier signals at the wavelengths 13.1 and 3.6 cm, a direct measure of the total electron content between spacecraft and ground station, was used to derive the electron density distribution in the solar corona. Linear power-law representations of the coronal electron density were derived for the range of solar distances from 4 solar radii to 40 solar radii on both sides of the Sun. The corona was found to be very nearly symmetric; the radial falloff exponent being 2.54 +/- 0.05 for occultation ingress (east solar limb) and 2.42 +/- 0.05 for egress (west limb), respectively. The departure of these exponents from the inverse equare relation implies that significant solar wind acceleration is occurring within the radial range of the observations. The electron density level was found to be considerably lower than that observed during the 1988 December solar occultation of Voyager 2. Although the smoothed sunspot number R(sub z) (a standard indicator of solar activity) was almost the same in 1988 December and 1991 August, the mean electron density at 20 solar radii was found to be 1.7 +/- 0.1 x 10(exp 3)/cu cm during the Ulysses conjunction, a decline by almost a factor of 4 from the value obtained during the Voyager conjunction.

Description: One of the long-standing uncertainties in the wave-resonance theory of coronal heating is the stability of the resonance layer. The wave motions in the resonance layer produce highly localized shear flows which vary sinusoidally in time with the resonance period. This configuration is potentially susceptible to the Kelvin-Helmholtz instability (KHI), which can enhance small-scale structure and turbulent broadening of shear layers on relatively rapid ideal timescales. We have investigated numerically the response of a characteristic velocity profile, derived from resonance absorption models, to finite fluid perturbations comparable to photospheric fluctuations. We find that the KHI primarily should affect long (approximately greater than 6 x 10(exp 4) km) loops where higher velocity flows (M approximately greater than 0.2) exist in resonance layers of order 100 km wide. There, the Kelvin-Helmholtz growth time is comparable to or less than the resonance quarter-period, and the potentially stabilizing magnetic effects are not felt until the instability is well past the linear growth stage. Not only is the resonance layer broadened by the KHI, but also the convective energy transport out of the resonance layer is increased, thus adding to the efficiency of the wave-resonance heating process. In shorter loops, e.g., those in bright points and compact flares, the stabilization due to the magnetic field and the high resonance frequency inhibit the growth of the Kelvin-Helmholtz instability beyond a minimal level.

Description: The solar flare and coronal mass ejection (CME) events associated with the large and complex March 1989 active region are discussed. This active region gave us a chance to study the relation of CME with truly major solar flares. The work concentrates on questions of the relation of CMEs and flares to one another and to other types of activity on the Sun. As expected, some major (X-3B class) flares had associated CMEs. However, an unexpected finding is that others did not. In fact, there is strong evidence that the X4-4B flare of March 9th had no CME. This lack of a CME for such an outstanding flare event has important implications to theories of CME causation.Apparently, not all major flares cause CMEs or are caused by CMEs. The relations between CMEs and other types of solar activity are also discussed. No filament disappearances are reported for major CMEs studied here. Comparing these results with other studies, CMEs occur in association with flares and with erupting prominences, but neither are required for a CME. The relation between solar structures showing flaring without filament eruptions and structures showing filament eruptions without flares becomes important. The evolutionary relation between an active flaring sunspot region and extensive filaments without sunspots is reviewed, and the concept of an 'evolving magnetic structure' (EMS) is introduced. It is suggested that all CMEs arise in EMSs and that CMEs provide a major path through which azimuthal magnetic fields escape form the Sun during the solar cycle.

Description: There have been many significant improvements in the public access to the Space Shuttle Earth Observations Photography Database. New information is provided for the user community on the recently released videodisc of this database. Topics covered included the following: earlier attempts; our first laser videodisc in 1992; the new laser videodisc in 1994; and electronic database access.

Description: The Challenge Awards are designed to provide a unique perspective to students gifted in the arts and humanities from which to understand scientific endeavor by giving students an opportunity to participate in an ongoing research project. In the graduate program, seven students who had participated in previous Challenge Awards programs were selected to help develop the tools for Earth observations for the astronauts on the Space Radar Laboratory (SRL) missions. The goal of the Challenge Awards program was to prepare a training manual for the astronauts on the SRL missions. This paper describes the observations to be made by the astronauts on the SRL missions. The emphasis is on the dynamic seasonal features of the Earth's surface and atmosphere which justify the need for more than one flight of the SRL. Complete notebooks of the sites, global seasonal patterns, examples of radar and the Measurement of Air Pollution from Satellites data, and shuttle photographs have been given to each of the SRL crews.

Description: The large gradual solar-energetic-particle (SEP) events, where abundances are commonly measured, are produced when coronal mass ejections (CMEs) drive shock waves through the corona and the interplanetary medium. The shock accelerates particles from the highly-ionized, approximately 1.5 MK, plasma in a manner that depends only weakly upon the Q/A of the ion, except at very high energies. Averaging the approximately 1 MeV/amu abundances over many events compensates for the acceleration effects to produce abundances that appear to correspond directly to those in the coronal source for all observed elements, including H. The resulting abundances reflect the 4 x enhancement of ions with low values of first ionization potential (FIP) arising from ion-neutral fractionation that occurs as the atoms are transported up from the photosphere. A different pattern of fractionation is found for ions that are shock-accelerated from the high speed solar wind emerging from coronal holes.

Description: MacSigma0 is an interactive tool for the Macintosh which allows you to display and make computations from radar data collected by the following sensors: the JPL AIRSAR, ERS-1, JERS-1, and Magellan. The JPL AIRSAR system is a multi-polarimetric airborne synthetic aperture radar developed and operated by the Jet Propulsion Laboratory. It includes the single-frequency L-band sensor mounted on the NASA CV990 aircraft and its replacement, the multi-frequency P-, L-, and C-band sensors mounted on the NASA DC-8. MacSigma0 works with data in the standard JPL AIRSAR output product format, the compressed Stokes matrix format. ERS-1 and JERS-1 are single-frequency, single-polarization spaceborne synthetic aperture radars launched by the European Space Agency and NASDA respectively. To be usable by MacSigma0, The data must have been processed at the Alaska SAR Facility and must be in the "low-resolution" format. Magellan is a spacecraft mission to map the surface of Venus with imaging radar. The project is managed by the Jet Propulsion Laboratory. The spacecraft carries a single-frequency, single-polarization synthetic aperture radar. MacSigma0 works with framelets of the standard MIDR CD-ROM data products. MacSigma0 provides four basic functions: synthesis of images (if necessary), statistical analysis of selected areas, analysis of corner reflectors as a calibration measure (if appropriate and possible), and informative mouse tracking. For instance, the JPL AIRSAR data can be used to synthesize a variety of images such as a total power image. The total power image displays the sum of the polarized and unpolarized components of the backscatter for each pixel. Other images which can be synthesized are HH, HV, VV, RL, RR, HHVV*, HHHV*, HVVV*, HHVV* phase and correlation coefficient images. For the complex and phase images, phase is displayed using color and magnitude is displayed using intensity. MacSigma0 can also be used to compute statistics from within a selected area. The statistics computed depend on the image type. For JPL AIRSAR data, the HH, HV, VV, HHVV* phase, and correlation coefficient means and standard deviation measures are calculated. The mean, relative standard deviation, minimum, and maximum values are calculated for all other data types. A histogram of the selected area is also calculated and displayed. The selected area can be rectangular, linear, or polygonal in shape. The user is allowed to select multiple rectangular areas, but not multiple linear or polygonal areas. The statistics and histogram are displayed to the user and can either be printed or saved as a text file. MacSigma0 can also be used to analyze corner reflectors as a measure of the calibration for JPL AIRSAR, ERS-1, and JERS-1 data types. It computes a theoretical radar cross section and the actual radar cross section for a selected trihedral corner reflector. The theoretical cross section, measured cross section, their ratio in dBs, and other information are displayed to the user and can be saved into a text file. For ERS-1, JERS-1, and Magellan data, MacSigma0 simultaneously displays pixel location in data coordinates and in latitude, longitude coordinates. It also displays sigma0, the incidence angle (for Magellan data), the original pixel value (for Magellan data), and the noise power value (for ERS-1 and JERS-1 data). Grey scale computed images can be saved in a byte format (a headerless format which saves the image as a string of byte values) or a PICT format (a standard format readable by other image processing programs for the Macintosh). Images can also be printed. MacSigma0 is written in C-language for use on Macintosh series computers. The minimum configuration requirements for MacSigma0 are System 6.0, Finder 6.1, 1Mb of RAM, and at least a 4-bit color or grey-scale graphics display. MacSigma0 is also System 7 compatible. To compile the source code, Apple's Macintosh Programmers Workbench (MPW) 3.2 and the MPW C language compiler version 3.2 are required. The source code will not compile with a later version of the compiler; however, the compiled application which will run under the minimum hardware configuration is provided on the distribution medium. In addition, the distribution media includes an executable which runs significantly faster but requires a 68881 compatible math coprocessor and a 68020 compatible CPU. Since JPL AIRSAR data files can be very large, it is often desirable to reduce the size of a data file before transferring it to the Macintosh for use in MacSigma0. A small FORTRAN program which can be used for this purpose is included on the distribution media. MacSigma0 will print statistics on any output device which supports QuickDraw, and it will print images on any device which supports QuickDraw or PostScript. The standard distribution medium for MacSigma0 is a set of five 1.4Mb Macintosh format diskettes. This program was developed in 1992 and is a copyrighted work with all copyright vested in NASA. Version 4.2 of MacSigma0 was released in 1993.

Description: VICAR (Video Image Communication and Retrieval) is a general purpose image processing software system that has been under continuous development since the late 1960's. Originally intended for data from the NASA Jet Propulsion Laboratory's unmanned planetary spacecraft, VICAR is now used for a variety of other applications including biomedical image processing, cartography, earth resources, and geological exploration. The development of this newest version of VICAR emphasized a standardized, easily-understood user interface, a shield between the user and the host operating system, and a comprehensive array of image processing capabilities. Structurally, VICAR can be divided into roughly two parts; a suite of applications programs and an executive which serves as the interfaces between the applications, the operating system, and the user. There are several hundred applications programs ranging in function from interactive image editing, data compression/decompression, and map projection, to blemish, noise, and artifact removal, mosaic generation, and pattern recognition and location. An information management system designed specifically for handling image related data can merge image data with other types of data files. The user accesses these programs through the VICAR executive, which consists of a supervisor and a run-time library. From the viewpoint of the user and the applications programs, the executive is an environment that is independent of the operating system. VICAR does not replace the host computer's operating system; instead, it overlays the host resources. The core of the executive is the VICAR Supervisor, which is based on NASA Goddard Space Flight Center's Transportable Applications Executive (TAE). Various modifications and extensions have been made to optimize TAE for image processing applications, resulting in a user friendly environment. The rest of the executive consists of the VICAR Run-Time Library, which provides a set of subroutines (image I/O, label I/O, parameter I/O, etc.) to facilitate image processing and provide the fastest I/O possible while maintaining a wide variety of capabilities. The run-time library also includes the Virtual Raster Display Interface (VRDI) which allows display oriented applications programs to be written for a variety of display devices using a set of common routines. (A display device can be any frame-buffer type device which is attached to the host computer and has memory planes for the display and manipulation of images. A display device may have any number of separate 8-bit image memory planes (IMPs), a graphics overlay plane, pseudo-color capabilities, hardware zoom and pan, and other features). The VRDI supports the following display devices: VICOM (Gould/Deanza) IP8500, RAMTEK RM-9465, ADAGE (Ikonas) IK3000 and the International Imaging Systems IVAS. VRDI's purpose is to provide a uniform operating environment not only for an application programmer, but for the user as well. The programmer is able to write programs without being concerned with the specifics of the device for which the application is intended. The VICAR Interactive Display Subsystem (VIDS) is a collection of utilities for easy interactive display and manipulation of images on a display device. VIDS has characteristics of both the executive and an application program, and offers a wide menu of image manipulation options. VIDS uses the VRDI to communicate with display devices. The first step in using VIDS to analyze and enhance an image (one simple example of VICAR's numerous capabilities) is to examine the histogram of the image. The histogram is a plot of frequency of occurrence for each pixel value (0 - 255) loaded in the image plane. If, for example, the histogram shows that there are no pixel values below 64 or above 192, the histogram can be "stretched" so that the value of 64 is mapped to zero and 192 is mapped to 255. Now the user can use the full dynamic range of the display device to display the data and better see its contents. Another example of a VIDS procedure is the JMOVIE command, which allows the user to run animations interactively on the display device. JMOVIE uses the concept of "frames", which are the individual frames which comprise the animation to be viewed. The user loads images into the frames after the size and number of frames has been selected. VICAR's source languages are primarily FORTRAN and C, with some VAX Assembler and array processor code. The VICAR run-time library is designed to work equally easily from either FORTRAN or C. The program was implemented on a DEC VAX series computer operating under VMS 4.7. The virtual memory required is 1.5MB. Approximately 180,000 blocks of storage are needed for the saveset. VICAR (version 2.3A/3G/13H) is a copyrighted work with all copyright vested in NASA and is available by license for a period of ten (10) years to approved licensees. This program was developed in 1989.