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  • Electron microscopy  (38)
  • Lepidoptera  (31)
  • Springer  (69)
  • American Institute of Physics (AIP)
  • International Union of Crystallography (IUCr)
  • National Academy of Sciences
  • Taylor & Francis
  • 1980-1984  (69)
  • 1950-1954
  • 1981  (69)
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  • Springer  (69)
  • American Institute of Physics (AIP)
  • International Union of Crystallography (IUCr)
  • National Academy of Sciences
  • Taylor & Francis
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  • 1980-1984  (69)
  • 1950-1954
Year
  • 1
    Electronic Resource
    Electronic Resource
    The effect of variation in host plant on the growth of an oligophagous insect, Malacosoma americanum and its polyphagous relative, Malacosoma disstria (1981)
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 163-168 
    Details
    ISSN: 1570-7458
    Keywords: host-plant relations ; host variation ; Malacosoma americanum ; Malacosoma disstria ; Lasiocampidae ; Geometridae ; Lepidoptera ; niche breadth ; specialization ; plantinsect interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Raupen von Malacosoma americanum (F.), einer oligophagen Art, die sich vor allem auf Prunus und andern baumartigen Rosaceen entwickelt, wurden verglichen mit Raupen der polyphageren Verwandten M. disstria Hb. und zwar im Hinblick auf deren Empfindlichkeit auf Unterschiede im Blatt ihrer gemeinsamen Wirtspflanze, Prunus serotina Ehrh. Das Puppengewicht und die Entwicklungszeit bis zur Verpuppung wurden gemessen bei Raupen, welche auf Blättern von freiwachsenden und von beschatteten Jungpflanzen gezüchtet worden waren. Die Blattunterschiede hatten eine ausgesprochene Wirkung, aber es gab keine Unterschiede in der Reaktion der beiden Arten. Dieser Vergleich lässt vermuten, dass die Empfindlichkeit auf intraspezifische Unterschiede der Wirtspflanzenqualität bei wirtsspezifischen und polyphagen Arten gleich ist. Indessen dürften sich laut anderen Resultaten einige Insektenarten anders verhalten.
    Notes: Abstract Larvae of Malacosoma americanum (F.)(Lepidoptera: Lasiocampidae) an oligophagous species that feeds primarily on Prunus and other rosaceous trees, were compared to larvae of the more highly polyphagous congener M. disstria Hb., with respect to their sensitivity to variation in the foliage of a common host plant, Prunus serotina Ehrh. Pupal weight and time to pupation were measured on larvae reared on foliage from open-grown and from shaded saplings. The difference in foliage had a pronounced effect, but no difference was evident between the species in their response to the variation in foliage. This comparison implies that sensitivity to intraspecific variation in host quality does not differ between host-specific and generalized species. However, results from other species suggest that some species of insects do differ in this respect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300882
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  • 2
    Electronic Resource
    Electronic Resource
    Food plant specialization and feeding efficiency in the tent caterpillars Malacosoma disstria and M. americanum (1981)
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 106-110 
    Details
    ISSN: 1570-7458
    Keywords: assimilation efficiency ; growth efficiency ; niche breadth ; specialization ; hostplant relations ; plant-insect interactions ; Malacosoma americanum ; Malacosoma disstria ; Lasiocampidae ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Raupen von Malacosoma americanum, einer oligophagen Art, die sich vor allem auf Prunus und andern baumartigen Rosaceen. entwickelt, wurden mit Raupen der polyphageren Verwandten M. disstria in Hinblick auf die Verwertung der Blätter ihres gemeinsames Wirtes Prunus serotina verglichen. Wirerhielten ähnliche Werte wie sie früher für andere Lepidopteren publiziert worden waren und zwar in Bezug auf die üblichen Messwerte, Anteil verwertete Nahrung, Wachstum in Trockengewicht pro Einheit gefressene oder verwertete Nahrung. Zudem unterschieden sich die beiden Arten in keiner Masszahl für Effizienz. Unsere Resultate sind im Einklang mit der Folgerung, das spezialisierte phytophage Insekten ihre Wirtspflanzen nicht besser ausnützen als as polyphage Arten tun.
    Notes: Abstract Larvae of Malacosoma americanum (F.) an oligophagous species that feeds primarily on Prunus and other rosaceous trees, were compared to larvae of the more highly polyphagous congener M. disstria Hb., with respect to the efficiency of utilization of the foliage of a common host plant, Prunus serotina Ehrh. We obtained values similar to those reported for other Lepidoptera for the commonly used measures of the fraction of ingested food that was assimilated, and for the growth in dry weight per unit of food ingested or assimilated. Moreover, the two species did not differ in any measure of efficiency. Our results are compatible with the conclusion that specialized phytophagous insects do not use their host plants with greater physiological efficiency than do generalized insects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300873
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  • 3
    Electronic Resource
    Electronic Resource
    Host races and sympatric speciation in small ermine moths, Yponomeutidae (1981)
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 280-292 
    Details
    ISSN: 1570-7458
    Keywords: Allozyme variation ; Lepidoptera ; Yponomeuta padellus ; evolutionary stages ; F-statistics ; panmixis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Le degré de différenciation génétique en allozymes à des niveaux divers de divergence évolutive (populations conspécifiques, sibling species et non-sibling species) d'Yponomeuta a été déterminé. Les distances génétiques entre des sibling species ou des non-sibling species sont très larges. Il en est conclu que de telles estimations ne fournissent pas beaucoup d'informations sur le phénomène de spéciation même. Des coéfficients de reproduction consanguine (F ST) ont été calculés pour des populations d'Y. padellus provenant de six plantes-hôtes. La valeur moyenne F STest .030±.012. Les valuers de F ST des populations recueillies sur Crataegus, (plante-hôte habituelle d'Y. padellus), sont inférieures 2–3.5 fois à celles des populations de l'ensemble des autres plantes-hôtes. L'apparition de races en fonction de l'hôte, mesurée par les différences importantes dans de fréquences des allozymes entre populations sympatriques sur plusieurs plantes-hôtes, a été examinée dans quatre régions. Il apparaît ainsi que la formation de races en fonction de l'hôte se produit chez Y. padellus et que la spéciation sympatrique est un évènement très vraisemblable.
    Notes: Abstract The amount of genetic differentiation at various levels of evolutionary divergence (conspecific populations, sibling species and non-sibling species) in Yponomeuta was determined. Genetic distances between siblings or non-siblings were found to cover a wide range. It is concluded that such estimates do not give much information on the speciation process itself. Inbreeding coefficients were calculated for populations of Y. padellus from a total of six host plants. The grand mean F ST value is 0.030±0.012. F ST values for populations sampled from Crataegus, the common food plant of Y. padellus, are 2–3.5 times smaller than those for populations from the other food plants taken together. Host race formation, as measured by significant differences in allozyme frequencies between sympatric populations on two or more food plants, was investigated in four areas. Host race formation seems to occur in Y. padellus and sympatric speciation is a likely event.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00347708
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  • 4
    Electronic Resource
    Electronic Resource
    Repellency of pheromones released by females of Heliothis armigera and H. zea to females of both species (1981)
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 123-127 
    Details
    ISSN: 1570-7458
    Keywords: Sex Pheromones ; Repellent ; Heliothis armigera ; Heliothis zea ; Lepidoptera ; Noctuidae ; cotton bollworm ; corn earworm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'examen en olfactomètre a porté sur les réactions face à d'autres femelles de la même espèce, de femelles vierges ou ayant copulé d'Heliothis armigera Hübner et H. zea Boddie. Le lot comprenait 8 femelles, vierges ou ayant copulé en présence d'une femelle vierge ou ayant copulé. Les 4 combinaisons possibles de femelles vierges et de femelles ayant copulé ont été examinées avec 12 répétitions pour chaque espèce. Un extrait de l'extrémité de l'abdomen de femelles vierges d'une espèce a été présenté aux femelles de l'autre espèce pour examiner les possibilités de réactions interspécifiques aux phéromones. Pour chaque espèce, les réactions interspécifiques de répulsion entre femelles ont été hautement significatives par rapport aux témoins, à l'exception toutefois des réactions de femelle ayant copulé face à des femelles ayant elles aussi copulé. Les répulsions moyennes chez H. armigera et H. zea pour les 8 femelles de chaque expérience ont été: a) vierges en présence d'une vierge: 7,33 et 7,66; b) vierges en présence d'une femelles ayant copulé: 5,76 et 5,58; c) femelles ayant copulé en présence d'une vierge: 4,67 et 4,83. Les différences sont hautement significatives entre chaque paire de moyennes et entre chaque paire et le lot témoin; 3,17; 3,17; 3,42; 4,00 pour H. armigera; 3,17; 3,50; 2,83 et 3,75 pour H. zea. Les femelles vierges des deux espèces, H. armigera et H. zea ont présenté une réaction de répulsion en présence d'un extrait de l'abdomen de l'autre espèce; les répulsions moyennes étant respectivement 5,53 et 5,33 contre 3,83 et 3,58 pour le lot trémoin. On peut en conclure que ces répulsions doivent entraîner une tendance à la répartition uniforme.
    Notes: Abstract An olfactometer was used to determine the effect of pheromones released by females of the bollworms Heliothis armigera (Hübner) and H. zea (Boddie) on females of the same species. Four combinations of virgin and mated females were tested for repellency of one to the other. Evidence is presented that females of the two bollworms were repelled by females of the same species. In addition, extracts of virgin female abdomens of each species repelled virgin females of the other species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300876
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  • 5
    Electronic Resource
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    Effects of cytochalasin B and dihydrocytochalasin B on calcium transport by intestinal absorptive cells (1981)
    Springer
    Calcified tissue international 33 (1981), S. 143-151 
    Details
    ISSN: 1432-0827
    Keywords: Calcium transport ; Cytochalasin B ; Dihydrocytochalasin B ; Colchicine ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary In vivo calcium absorption was studied in normal and rachitic chicks. Cytochalasin B (CB) at a concentration of 25 µg/ml added to the medium inside the duodenal lumen inhibited calcium absorption (20 min) from 82.5±1.9% of calcium absorbed in the controls to 59.2±3% in normal and from 70.0±2.3% to 47.0±2.1% in rachitic chicks. In vitro studies by everted ileal sacs of young rabbits also showed an inhibition of active transport of calcium due to CB. Whereas in the controls the ratio of45Ca concentrations in serosal and mucosal media (60 min) was 7.2±0.32, the ratios were 5.24±0.52; 4.40±0.36; 3.40±0.42; 5.77±0.52; 1.38±0.08; and 1.06±0.02 in the presence of CB at concentrations of 5, 10 and 25 µg/ml; colchicine 10−4M, Na citrate 0.02M, and heat-devitalized conditions, respectively.45Ca concentration in the mucosal scrapings was also affected. It showed an increase from controls (15,101±404 cpm/mg) and correlated with CB concentration: 17,378±489, 19,015±1000, and 20,201±362 at 5, 10, and 25 µg/ml, respectively. Dihydrocytochalasin B also inhibited active calcium transport and caused an increase in45Ca concentration in the mucosal scrapings. Correlated electron microscopic studies showed certain changes in the brush border, especially in some actin microfilaments in the terminal web region. It seems that these morphological alterations may be related to transcytoplasmic movement of calcium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02409427
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  • 6
    Electronic Resource
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    Ultrastructure of the rat epiphyseal growth plate following chronic ethanol ingestion (1981)
    Springer
    Calcified tissue international 33 (1981), S. 381-384 
    Details
    ISSN: 1432-0827
    Keywords: Alcohol ; Electron microscopy ; Growth plate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary We have previously demonstrated that ethanol has a direct toxic effect on the rat skeleton characterized by decreased trabecular bone volume. In the present study, we examined the ultrastructure of the distal radial epiphyseal growth plates in these same animals. Eight weeks of ethanol administration to 12 male rats results in serum alcohol levels of 140 mg/dl but did not alter the width or light microscopic appearance of the radial growth plate. Quantitative electron microscopy failed to demonstrate morphologic evidence of toxicity in the skeletal cells. We conclude that although ethanol appears to have a direct effect on rat bone characterized by enhanced resorption, toxicity is not attended by ultrastructural changes in the skeletal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02409460
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  • 7
    Electronic Resource
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    Biochemical and histological studies on various bone cell preparations (1981)
    Springer
    Calcified tissue international 33 (1981), S. 529-540 
    Details
    ISSN: 1432-0827
    Keywords: Bone cells ; Electron microscopy ; PTH ; PGE1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Four different cell populations—designated PF, OB, OC, and PC—were isolated from calvaria of 18-day-old chick embryos for analysis of the effects of hormones on bone tissue. The cell populations were studied with histological and biochemical methods. Apart from the well-known cell types present in calvaria, a new cell type was found in the noncalcified organic matrix between the osteoblastic layer and the calcified matrix. These cells were provisionally called osteocytic osteoblasts. They represent the “transition state” between osteoblasts and osteocytes. On the basis of histological studies with light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM), the PF population was considered to originate primarily from the periosteal fibroblasts, the OB population from the osteoblasts and osteocytic osteoblasts. The population of cells still present in calvaria after removal of periosteal fibroblasts and osteoblasts was called the OC population. This cell population was very much enriched with osteocytes. The fourth isolated population (PC) was a mixed population of fibroblasts, osteoblasts, and preosteoblasts. On exposure to parathyroid hormone (PTH), all four cell populations showed increased lactate production, but only the OB and OC populations displayed increased cAMP production. Prostaglandin E1 (PGE1) stimulated cAMP production in both OB and PF cells. From the results of this study it was concluded that PTH receptors are present on all of the cell types studied, but that occupancy of the receptor induces adenylate cyclase stimulation only in osteocytes and fully differentiated osteoblasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02409485
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  • 8
    Electronic Resource
    Electronic Resource
    Crystal orientation in the shell of the domestic fowl: An electron diffraction study (1981)
    Springer
    Calcified tissue international 33 (1981), S. 119-124 
    Details
    ISSN: 1432-0827
    Keywords: Avian eggshell ; Microstructure ; Electron microscopy ; Electron diffraction ; Calcite growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The eggshell of the domestic fowl has been studied by transmission electron microscopy and diffraction. Thin sections of shell were prepared by chemical and ion-beam thinning techniques. Each calcite column of the palisade layer consisted of crystallites of diameter 20 to 30 µm with some tendency for crystallite alignment within a single column. Evidence indicates that there was no significant preferred orientation in the palisade layer as a whole. Only in the surface layer was any preferred orientation detected, and here {1014} planes tended to lie parallel to the surface. The results are compared with previously published data, and calcite nucleation and growth are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02409423
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  • 9
    Electronic Resource
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    Variable planar particle arrangements in the photosynthetic membrane of Rhodopseudomonas viridis (1981)
    Springer
    European biophysics journal 7 (1981), S. 209-212 
    Details
    ISSN: 1432-1017
    Keywords: Photosynthetic bacteria ; Electron microscopy ; Planar lattices
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The thylakoids of Rhodopseudomonas viridis have been studied by freeze-fracturing whole cells. Depending on growth conditions and treatment before freezing, three different types of particle arrangements in the photosynthetic membrane are reported: a random arrangement, an isometric (quadratic) lattice arrangement with a lattice constant of 12.5 ± 0.8 nm, and a hexagonal lattice arrangement with a lattice constant of 12.5 ± 0.8 nm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00539181
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  • 10
    Electronic Resource
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    Freeze fracture studies of reaction centers from Rhodospirillum rubrum in chromatophores and liposomes (1981)
    Springer
    Archives of microbiology 130 (1981), S. 125-128 
    Details
    ISSN: 1432-072X
    Keywords: Rhodospirillum rubrum ; Chromatophores ; Reaction centers ; Liposomes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In freeze-fractures of chromatophores of Rhodospirillum rubrum the reaction centers are seen as hexagonal arranged particles of 13 nm diameter with a density of around 5,500 particles per μm2. Similar regions on the cytoplasmic membrane suggest that these parts are the prospective invagination sites. Isolated reaction centers are easily incorporated into liposomes. In freeze fractures of liposomes particles similar in shape and size, although less dense as in chromatophores are observed. In negative staining much smaller units of only 5 nm in diameter are found indicating that reaction centers occur in the membrane as tri- or tetramers. There is a strong correlation between particle density in chromatophores and titratable reaction centers remaining in these membranes after extraction of reaction centers by detergents; both values are in good agreement with the yield of reaction centers at a given detergent concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411063
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  • 11
    Electronic Resource
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    Relative numbers of selected bacterial forms in different regions of the cockroach hindgut (1981)
    Springer
    Archives of microbiology 129 (1981), S. 129-134 
    Details
    ISSN: 1432-072X
    Keywords: Cockroach ; Hindgut ; Distribution ; Microbial morphotypes ; Transmission ; Electron microscopy ; Statistical analysis ; Eublaberus posticus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relative numbers of fourteen microbial morphotypes in transmission electron micrographs of the hindgut of a cockroach, Eublaberus posticus, were counted and their distribution was analyzed statistically. The microbiota of three wall-associated regions (the anterior paunch, the posterior paunch, and the black band region) was clearly different from that of the gut lumen. The three wall fractions were also significantly different from each other. Only one of the fourteen types, prosthecate bacteria, appeared to be distributed randomly in the four fractions. The five main wall-associated morphotypes individually constituted up to 41% of the microbes in some micrographs. They included one type with the characteristic morphology of Methanospirillum. Six morphotypes rarely made up over 2% of the population, but were consistently present. The numbers of the remaining three morphotypes were quite variable between micrographs and between individual insects, but when present often made up 5–10% of the population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455348
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  • 12
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    Entrapment and lysis of the cyanobacterium Phormidium luridum by aqueous colonies of Myxococcus xanthus PCO2 (1981)
    Springer
    Archives of microbiology 129 (1981), S. 285-294 
    Details
    ISSN: 1432-072X
    Keywords: Biological control ; Cyanobacteria ; Electron microscopy ; Entrapment ; lysis ; Myxococcus ; Phormidium ; Spherule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Myxococcus xanthus isolate from a farm drainage ditch, designated strain PCO2, is capable of rapidly inducing lysis of both agar and liquid-grown cultures of the cyanobacterium, Phormidium luridum, var. olivacea. Microscopic studies of the predator-prey interaction demonstrate that lysis of the cyanobacterium occurs within clumps and spherules formed by the cells of M. xanthus PCO2. In the earliest stage, one sees the formation of irregular microclumps of bacteria and cyanobacterial filaments. As these clumps mature, colonies 1 to 6 mm in diameter develops. The center of these densely green colonies contains cyanohacteria in various stages of degradation, while the periphery is almost exclusively a tightly woven mass of myxobacterial cells. Electron microscopy shows that long extrusions from the outer membrane of the M. xanthus PCO2 cells are involved in the formation both of initial clumps and of mature colonial spherules. These extrusions appear to efficiently entangle the cyanobacterial filaments in the culture environment. Predator-to-prey ratios of 1/10, 1/100 and 1/1,000 have resulted in cyanobacterial lysis. Because the entrapment and lysis of P. luridum filaments by M. xanthus PCO2 appears to be independent of any other heterotrophic nutritional requirement, as well as of environmental agitation, this system has potential as a biological control technique for undesirable aquatic cyanobacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414699
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  • 13
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    Electron microscopy and X-ray microanalysis of a halophilic leptospire (1981)
    Springer
    Archives of microbiology 130 (1981), S. 339-343 
    Details
    ISSN: 1432-072X
    Keywords: Leptospira ; Halophilic ; Electron microscopy ; X-ray analysis ; Inclusions ; Cytoplasmic tubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The morphology of cells of strain Muggia, a slightly halophilic leptospire, was examined by the negative staining technique. The ultrastructure of the cells was rather similar to that of cells of Leptonema illini, i. e. the cells possessed cytoplasmic tubules. The basal complex of their flagella, however, was similar to the corresponding part of flagella on Gramnegative bacteria. The interior of the cells was densely packed with inclusions, except for the two outermost wavelengths at each end where these inclusions were absent. X-ray microanalysis showed that the inclusions contained sodium and chlorine as their main constituents. The inclusions disappeared upon storage of the cultures at room temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414596
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  • 14
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    Molecular weight and quaternary structure of ribulose bisphosphate carboxylase from the cyanobacterium, Synechococcus sp. (1981)
    Springer
    Archives of microbiology 130 (1981), S. 344-348 
    Details
    ISSN: 1432-072X
    Keywords: Ribulose bisphosphate carboxylase ; Quaternary structure ; Molecular weight ; Electron microscopy ; Cyanobacteria ; Synechococcus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ribulose bisphosphate (RuP2) carboxylase from the marme cyanobacterium, Synechococcus sp., comprised both large (57,000 dalton) and small (12,000 dalton) subunits. The undissociated, purified enzyme was considerably smaller than the spinach enzyme when compared by pore-gradient electrophoresis, gel filtration and density-gradient centrifugation. This suggested that the cyanobacterial enzyme might have a hexameric (L6S6) subunit structure, unlike the enzymes from spinach and many other organisms which are octamers (L8S8). However, the molecular weight of the Synechococcus enzyme was measured by equilibrium sedimentation and found to be 530,000, which is within the range observed for L8S8-type enzymes. Furthermore, electron microscopic studies of negatively stained preparations of both the native enzyme, and a preparation depleted of 87% of its small subunits by repeated mild-acid precipitation, revealed four-fold symmetry characteristic of an octameric, cubical structure. Synechococcus RuP2 carboxylase therefore must be an L8S8 octamer and its anomalous pore-penetration behaviour may be due to an asymmetric shape. Some support for the latter possibility was provided by electron miscoscopic observations of two different types of images which may be different views of the molecule in two planes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414597
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  • 15
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    Baldcypress allelochemics and the inhibition of silkworm enteric microorganisms Some Ecological Considerations (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 103-114 
    Details
    ISSN: 1573-1561
    Keywords: Baldcypress ; Taxodium distichum ; 2-furaldehyde ; 2-furoic acid ; Bombyx mori ; Lepidoptera ; Bombycidae ; enteric microbial inhibition ; Bacillus ; Micrococcus ; Arthrobacter ; Mucor ; Curvularia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Exposure ofBombyx mori larvae to 2-furaldehyde, a major volatile component of baldcypress heartwood,Taxodium distichum, resulted in in vivo inhibition of enteric microorganisms at concentrations (1–47 ppm) that were released naturally from heartwood. The 7 bacterial and 2 fungal enteric isolates were also inhibited in vitro at the same concentrations. It is suggested that inhibition of leaf surface micro-organisms or in vivo inhibition of silkworm enteric microflora, as a result of indirect or direct action of 2-furaldehyde, exacerbates the growth-inhibitory effects of this compound on larvae by reducing the microbial nutritional contribution. The ecological significance of insect enteric microbial inhibition by plant allelochemics is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00988638
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  • 16
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    Attractant lures for males of the pea moth,Cydia nigricana (F.) Containing (E)-10-dodecen-1-yl acetate and (E,E)-8,10-dodecadien-1-yl acetate (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 563-573 
    Details
    ISSN: 1573-1561
    Keywords: Pea moth ; Cydia nigricana (F.) ; Lepidoptera ; Olethreutidae ; sex attractant ; (E)-10-dodecen-1-yl acetate ; (E,E)-8 ; 10-dodecadien-1-yl acetate ; lure ; formulation ; antioxidant ; monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Lures containing different amounts of (E)-10-dodecen-1-yl acetate and (E,E)-8, 10-dodecadien-1-yl acetate were formulated in natural rubber stoppers or polyethylene vials and tested in traps in the field to establish relative attractiveness to males of the pea moth,Cydia nigricana (F.), dose-response relationships, and the effects of weathering. Initially, both formulations of (E,E)-8,10-dodecadien-1-yl acetate were much more attractive than those of (E)-10-dodecen-1-yl acetate, but their activity diminished considerably after only 5 days in the field because an inhibitor was formed, and polyethylene formulations were not attractive after one week. However, lures containing (E)-10-dodecen-1-yl acetate, with or without antioxidants, in both rubber and polyethylene were still attractive after three months' exposure in the field and are therefore suitable for practical monitoring of pea moth populations.
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    URL: http://dx.doi.org/10.1007/BF00987704
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    Air permeation tests with (Z,Z)-3,13-octadecadien-1-ol acetate for reduction in trap catch of peachtree and lesser peachtree borer moths (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 575-582 
    Details
    ISSN: 1573-1561
    Keywords: Peachtree borer ; lesser peachtree borer ; Synanthedon exitiosa ; S. pictipes ; Lepidoptera ; Sessiidae ; Z,Z-3 ; 13-octadecadien-1-ol acetate sex pheromone ; traps ; air permeation ; trap catch reduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Air permeation trials were conducted to determine whether trap catches of the peachtree borer (PTB)Synanthedon exitiosa (Say) and lesser peachtree borer (LPTB)S. pictipes (Grote and Robinson) could be reduced through application of the synthetic pheromone of the PTB, (Z,Z)-3,13-octadecadien-1-ol acetate (Z,Z-ODDA), at 92% isomeric purity. The pheromone was released into the air of peach orchard test plots with hanging laminated plastic dispensers or hollow fiber dispensers in the trees and by aerial dispersal of microcapsules containing a solution of the lure. Results showed that all formulations ofZ, Z-ODDA reduced trap catches of both PTB and LPTB in treated plots relative to catches in traps in untreated plots. In one air permeation experiment, three types of laminated dispensers and one type of hollow fiber dispenser each reduced trap catch of PTB by 93–100% and of LPTB by 75–95%. In a separate test, two formulations of microencapsulatedZ,Z-ODDA did not reduce trap catch of male PTB as effectively as the laminated dispenser; results for LPTB were similar for both the dispensers and microcapsules. Catches of male PTB moths in traps baited with laminated and or fiber pheromone dispensers containing ODDA (94∶6%Z,Z∶E,Z) were not significantly different. LPTB male moths were also captured in these traps, the catches being dependent upon the initial pheromone loading and the type of dispensers.
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    URL: http://dx.doi.org/10.1007/BF00987705
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  • 18
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    Insect sex pheromones: Evaporation rates of alcohols and acetates from natural rubber septa (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 627-633 
    Details
    ISSN: 1573-1561
    Keywords: Insect sex pheromones ; insect sex attractants ; Lepidoptera ; pheromones ; pheromone formulations ; insect population monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The half-lives (t 1/2) of alcohol sex pheromones, 1-alkanols, acetate sex pheromones, and an epoxide (disparlure) were determined on natural rubber septa. Thet 1/2 values for the homologous alcohols from decanol to heptadecanol increased regularly from 2.2 to 1117 days, but thet 1/2 of octadecanol was 609 days. Thet 1/2 values of (Z)7-, (E)7-, and (Z)9-tetradecen-1-ol acetates were 154, 168, and 199 days, respectively, whereas those of five other tested 14-carbon acetates ranged from 310 to 350 days. The dependence oft 1/2 values on chain length and double-bond position is consistent with the hypothesis that molecular size is an important variable affectingt 1/2 values. Also, in accordance with the hypothesis, when aZ-alkenyl compound has a much shortert 1/2 than the corresponding saturated compound, thet 1/2 values of theZ compound and itsE isomer may be quite different. Thus, (E)-9-tetradecen-l-ol acetate had at 1/2 of 331 days. Thet 1/2 of disparlure was 180 days. The effect of thecis-7,8 epoxide group is apparently similar to that of the olefin group in lowering thet 1/2 below the value that would be expected solely on the basis of chain length.
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    URL: http://dx.doi.org/10.1007/BF00987710
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  • 19
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    Relative efficiency of sticky and cylindrical electrical grid traps in capturingSpodoptera exigua (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 733-740 
    Details
    ISSN: 1573-1561
    Keywords: Beet armyworm ; Spodoptera exigua ; Lepidoptera ; Noctuidae ; pheromone trap ; trap efficiency ; Pherocon 1C trap ; population monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The relative efficiency of sticky traps and cylindrical electrical grid traps for capturing male beet armyworm moths,Spodoptera exigua (Hubner), was studied using night vision equipment. The sticky and grid traps captured 34 and 40%, respectively, of the insects which oriented to them. The grid trap's capacity was limited only by the size of the receptacle (3.8-liter bucket) at the base of the funnel. The maximum capacity of the sticky trap was ca. 110 insects, and the efficiency of this trap declined rapidly when captures exceeded 50% capacity. This decline was caused by the physical blockage of the sticky surface by the captured insects. There was no evidence to indicate that captured males had any repelling effect on other males subsequently attracted to the sticky trap.
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    URL: http://dx.doi.org/10.1007/BF00990305
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    Kairomones and their use for management of entomophagous insects (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 909-917 
    Details
    ISSN: 1573-1561
    Keywords: Trichogramma pretiosum ; Hymenoptera ; Trichogrammatidae ; biological control ; kairomone ; Heliothis zea ; Lepidoptera ; Noctuidae ; host density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Trichogramma pretiosum Riley females exhibit success-motivated searching after oviposition. The stimulatory effect of contact with host eggs makes host-egg density an important factor in determining the appropriate strategy for behavioral manipulation, using kairomones, that simulate host seeking, in biological control programs. Host eggs can be used, in conjunction with kairomones or by themselves, to improve the performance of these important beneficial insects.
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    URL: http://dx.doi.org/10.1007/BF00987616
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  • 21
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    Adrenergic innervation of pancreatic islets and modulation of insulin secretion by the sympatho-adrenal system (1981)
    Springer
    Cell & tissue research 216 (1981), S. 15-30 
    Details
    ISSN: 1432-0878
    Keywords: Pancreatic islets ; Adrenergic innervation ; Insulin secretion ; Chemical sympathectomy ; Adrenalectomy ; Fluorescence histochemistry ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Morphological changes in the adrenergic innervation of pancreatic islets after chemical sympathectomy by use of 6-hydroxydopamine and the influence of the sympatho-adrenal system on insulin secretion were investigated in the mouse and rat. Fluorescence histochemistry revealed a clear-cut reduction in the number of adrenergic nerve fibers in the pancreatic islets 2 days after administration of 6-hydroxydopamine; the reduction was more pronounced in the rat than in the mouse. In the rat, a partial regeneration was seen after 6 weeks. In the pancreas of the mouse, after administration of 6-hydroxydopamine, a severe damage of unmyelinated nerve fibers was revealed electron microscopically. However, no ultrastructural or immunohistochemical alterations could be demonstrated in the endocrine cells of the islets. 6-Hydroxydopamine induced a depression of basal plasma insulin concentrations in mice and an elevation in rats. Adrenalectomy depressed basal plasma insulin levels in mice. The α-adrenoceptor antagonist phentolamine enhanced insulin secretion in normal mice. The secretory response of insulin to phentolamine was diminished by chemical sympathectomy and almost abolished by adrenalectomy or the combination of chemical sympathectomy and adrenalectomy. Thus, the effect of phentolamine is probably mediated by liberated catecholamines. It is concluded that basal insulin secretion is partially regulated by the sympatho-adrenal system and that species differences exist in this respect. In addition, the results suggest that endogenous catecholamines have the ability to promote insulin secretion.
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    URL: http://dx.doi.org/10.1007/BF00234541
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    The isolated perfused rabbit ovary — A model for studies of ovarian function (1981)
    Springer
    Cell & tissue research 216 (1981), S. 471-479 
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    ISSN: 1432-0878
    Keywords: Ovulation ; Perfusion ; Graafian follicle (Rabbit) ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the present investigation the ultrastructure of isolated rabbit ovaries, perfused with different media for various time periods, was studied. The steroid hormone production by the perfused ovary was also determined. Perfusion with Medium 199 results in prominent interstitial ovarian oedema which increases with perfusion time. Even after the addition of 6–10 % Dextran T40, oedema appears in the interstitial tissue of the ovary. Perfusion solutions with osmotically active colloid particles of large molecular size (Dextran T70; average molecular weight 70,000 and bovine serum albumin), cause less distortion in the ovarian structure, and ultrastructurally the ovarian tissues appear essentially the same as in the control ovaries. The results indicate that the perfused rabbit ovary, under strictly controlled conditions, can be used as an experimental model for studies of various aspects of ovarian function, including follicular rupture.
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    URL: http://dx.doi.org/10.1007/BF00238644
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    The basement membrane of the insect and crustacean compound eye: Definition, fine structure, and comparative morphology (1981)
    Springer
    Cell & tissue research 216 (1981), S. 205-214 
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    ISSN: 1432-0878
    Keywords: Compound eyes ; Insects ; Crustaceans ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The basement membrane of the compound eye of four insect species and three crustacean species was investigated employing electron microscopy. The basement membrane consists of an extracellular (basal lamina) and a cellular portion, the latter being composed of the flattened terminal extensions of cone cells and accessory pigment cells in insects and distal pigment cells in crustaceans. Other cells can also contribute to the basement membrane. It is thus a complex structure in all well-developed compound eyes. The cellular contributions vary in different species and were found to correlate to specific taxonomic units.
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    Ultrastructural Identification of gastrin-like immunoreactive nerve fibres in the brain of Xenopus laevis by means of colloidal gold or ferritin immunocytochemical methods (1981)
    Springer
    Cell & tissue research 216 (1981), S. 581-589 
    Details
    ISSN: 1432-0878
    Keywords: Gastrin ; CCK ; Median eminence ; Electron microscopy ; Xenopus laevis (Amphibia, Anura)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By use of an anti-gastrin serum and colloidal gold- or ferritin-labelled sheep anti-rabbit γ-globulins, nerve fibres and nerve terminals containing a gastrin-like substance were characterized at the ultrastructural level in the median eminence of Xenopus laevis. These immunoreactive fibres contain neurosecretory granules displaying medium to high electron density and a mean diameter of 75 nm. Labelling intensity varies from granule to granule. This is the first demonstration at the ultrastructural level of the precise location of a gastrin-like hormone in the median eminence of a vertebrate.
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    URL: http://dx.doi.org/10.1007/BF00238653
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    Ultrastructure of the pars intermedia of the adult sheep hypophysis (1981)
    Springer
    Cell & tissue research 217 (1981), S. 211-223 
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    ISSN: 1432-0878
    Keywords: Pituitary gland ; Pars intermedia ; Electron microscopy ; Sheep
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light microscopy of coronal sections of the sheep pars intermedia revealed a compact, incompletely lobulated V-shaped region about 15–20 cells thick, situated between the pars distalis and the pars nervosa. A prominent hypophysial cleft and follicles containing a colloid-like substance were seen. Using electron microscopy, five cell types could be distinguished: pars intermedia glandular cells, pars distalis-like glandular cells, interstitial cells, follicular cells and cleft lining cells. The polyhedral to pear-shaped pars intermedia glandular cells predominated. They contained dense-cored, membrane-bound granules near the Golgi complex, and larger, irregular vesicles with finely granular contents of varying electron density throughout the remaining cytoplasm; exocytotic release of granules was occasionally observed. Smaller numbers of cells resembling those seen in the pars distalis were scattered throughout the pars intermedia. Interstitial cells usually possessed elongated cytoplasmic processes which extended between the glandular cells, and were characterized by deeply indented nuclei, elaborate junctional complexes and an absence of cytoplasmic granules. Cells lining the follicles resembled the interstitial cells. The major cells bordering the hypophysial cleft were triangular in section and bore irregular microvilli on their free surface. The pars intermedia appeared to be less vascular than the remainder of the hypophysis and only occasional fenestrated capillaries were seen. Nerve profiles were rare.
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    URL: http://dx.doi.org/10.1007/BF00233839
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    Ultrastructural morphometry of blastogenesis (1981)
    Springer
    Cell & tissue research 215 (1981), S. 643-649 
    Details
    ISSN: 1432-0878
    Keywords: T-lymphocytes ; Blast cells ; Autoradiography ; Electron microscopy ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary As a companion to an earlier study, the morphometric attributes of stimulated (blast-derived) lymphocytes in mouse axillary lymph nodes have been established using stereological and autoradiographic methods. Blast transformation was induced in vivo with dinitrochlorobenzene (DNCB) and stimulated cells were labelled with tritiated thymidine. Random samples of cells were taken for light and electron microscopic morphometry. In comparison to the unstimulated lymphocyte, the stimulated cell increased in size and possessed a greater plasma membrane surface area. Increase in cell size was the result of increases in the volumes of all measured subcellular compartments both in the nucleus and the cytoplasm. Heterogeneity of the stimulated cell population precludes firm conclusions regarding the significance of all these ultrastructural changes, though alterations in cell surface are discussed in the context of known biochemical differences accompanying blastogenesis.
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    URL: http://dx.doi.org/10.1007/BF00233538
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    Morphological studies on neuroglia (1981)
    Springer
    Cell & tissue research 216 (1981), S. 557-568 
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    ISSN: 1432-0878
    Keywords: Electron microscopy ; Neuroglia ; Silver impregnation ; Brain ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The silver-impregnation procedure of Tsujiyama is suitable for demonstration of all three classical types of neuroglial cells; in the present study it was used for electron microscopic identification of neuroglial cells in the brain of the cat. The aim of the present study was 1) to determine impregnated structural correlates of neuroglial cells at the light- and electron-microscopic levels, and 2) to determine whether the method of Tsujiyama is applicable for the electron microscopic identification of the single types of neuroglial cells. Silver deposits were observed over the cytoplasm and processes of astrocytes where numerous glial filaments were present. Oligodendrocytes and microglial cells may be precisely differentiated by use of Tsujiyama's silver impregnation method at the electron microscopic level due to the pattern of silver-deposition in these two basic types of cells. This silver-impregnation method combined with electron microscopy is thus suitable for a precise identification of neuroglial cells; the technique may prove to be very helpful in identification of such categories of neuroglial cells that encompass also the images of cells which cannot be classified by use of the standard methods.
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    URL: http://dx.doi.org/10.1007/BF00238651
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    2-Furaldehyde from baldcypress (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 89-101 
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    ISSN: 1573-1561
    Keywords: Baldcypress ; Taxodium distichum ; 2-furaldehyde ; Bombyx mori ; Lepidoptera ; Bombycidae ; growth inhibition ; toxicity ; Georgia silkworm industry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract 2-Furaldehyde was growth inhibitory and toxic to larvae ofBombyx mori at concentrations as low as 1 ppm. Baldcypress,Taxodium distichum, heartwood released this volatile at concentrations in excess of these levels. The adverse effect of this chemical on silkworms may partially account for the demise of the Georgia silkworm industry (circa 1765), after the construction of a baldcypress rearing facility.
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    URL: http://dx.doi.org/10.1007/BF00988637
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    Synthesis of sex pheromone components of the forest tent caterpillar,Malacosoma disstria (Hübner) and of the western tent caterpillar,Malacosoma californicum (Packard) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 159-164 
    Details
    ISSN: 1573-1561
    Keywords: Synthesis ; isomers ; 5,7-dodecadienal ; sex pheromone ; Malacosoma disstria ; Malacosoma californicum ; Lepidoptera ; Lasiocampidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract All four geometrical isomers of 5,7-dodecadien-1-ol have been stereoselectively synthesized by using Wittig condensation reactions. (5 Z,7E)-5,7-Dodecadien-1-ol and its corresponding aldehyde are components of the sex pheromone of the forest tent caterpillar. (5 E,7 Z)-5,7-Dodecadienal is a component of the pheromone of the western tent caterpillar. These compounds have been successfully tested in the field.
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    Feeding preferences of spruce budworm (Choristoneura fumiferana Clem.) larvae to some host-plant chemicals (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 391-402 
    Details
    ISSN: 1573-1561
    Keywords: Choristoneura fumiferana ; Lepidoptera ; Tortricidae ; Abies balsamea ; feeding stimulants ; feeding deterrents ; feeding preference ; sugars ; glycosides ; amino acids ; organic acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Single-choice and three-choice tests were used to determine the relative importance of host-plant chemical extracts in eliciting feeding by spruce bud worm larvae. Water-soluble components of the host trees are the most important and, of these, the sugar and glycoside fractions are the most stimulating. The amino acid and organic base fractions have no apparent effect. The organic acid fractions deter feeding slightly.
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    A four-component pheromone blend for optimum attraction of redbacked cutworm males,Euxoa ochrogaster (Guenée) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 615-625 
    Details
    ISSN: 1573-1561
    Keywords: Redbacked cutworm ; Euxoa ochrogaster ; Lepidoptera ; Noctuidae ; sex pheromone blend ; sex attractant ; parapheromone ; Z-5-decenyl acetate ; Z-5-undecenyl acetate ; Z-5-dodecenyl acetate ; Z-7-dodecenyl acetate ; Z-9-dodecenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Four acetates,Z-5-decenyl acetate,Z-5-,Z-7-, andZ-9-dodecenyl acetates, in microgram ratios of 1∶200∶2∶1 or 1∶200∶6∶2 were excellent, specific sex pheromone blends for capturing male redbacked cutworm moths in cone traps. Blends in ratios of 1∶200∶2∶1 and 2∶200∶2∶1 at 1000 μg/ rubber septum dispenser remained highly effective for 6 weeks under field conditions. The essential minor components,Z-5-decenyl,Z-7-, andZ-9-dodecenyl acetates, became inhibitory at concentrations of about 10% in the blends, and this may be an important general phenomenon in lepidopteran pheromones. Blends involving a parapheromone,Z-5-undecenyl acetate, withZ-5-,Z-7-, andZ-9-dodecenyl acetate, in microgram ratios of 8∶200∶2∶1 or 20∶200∶6∶2 were also excellent specific attractants for this species. TheZ-8-dodecenyl acetate had no obvious effect on the attraction of the redbacked cutworm males.
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    Kairomones and their use for management of entomophagous insects: X. Laboratory studies on manipulation of host-finding behavior ofTrichogramma pretiosum riley1 with a kairomone extracted fromHeliothis zea (boddie) moth scales (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 635-648 
    Details
    ISSN: 1573-1561
    Keywords: Kairomone ; parasitoids ; biological control ; Trichogramma pretiosum ; Hymenoptera ; Trichogramma tidae ; Heliothis zea ; Lepidoptera ; Noeturidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The behavioral response ofTrichogramma pretiosum Riley females to the kairomone found inHeliothis zea (Boddie) moth scales is examined.
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    URL: http://dx.doi.org/10.1007/BF00987711
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    Sex pheromone of the saltmarsh caterpillar moth,Estigmene acrea (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 655-668 
    Details
    ISSN: 1573-1561
    Keywords: Saltmarsh caterpillar moth ; Estigmene acrea ; (Z,Z)-9,12-octadecadienal ; (Z,Z,Z)-9,12,15-octadecatrienal ; (Z,Z)-3,6-cis-9,10-epoxy-heneicosadiene ; fall webworm moth ; Hyphantria cunea ; insect sex pheromone ; cis-9,10-epoxyheneicosane ; trans-9,10-epoxyheneicosane ; linolealde-hyde ; linolenaldehyde ; Lepidoptera ; Arctiidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three compounds have been identified as components of the sex pheromone emitted by females of the saltmarsh caterpillar moth,Estigmene acrea (Drury). These are (Z,Z)-9,12-octadecadienal (I), (Z,Z,Z)-9,12,15-octadecatrienal (II), and (Z,Z)-3,6-cis-9,10-epoxyheneicosadiene (III). In female tip extract they were found in a ratio of 1∶6∶25, respectively, and in trapped female effluvia the ratio was 1∶6∶27, respectively. Combinations of III with either I or II elicited sustained upwind flight in a wind tunnel, but none of these compounds by themselves did so. There is evidence that the antennal acceptor site for III is chiral.
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    Comparative distribution and persistence of disparlure in woodland air after aerial application of three controlled-release formulations (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 867-880 
    Details
    ISSN: 1573-1561
    Keywords: Disparlure ; gypsy moth ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; controlled release ; laminated dispensers ; hollow fibers ; microcapsules ; atmospheric permeation ; mating disruption ; air concentration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Disparlure, the gypsy moth sex pheromone, was aerially applied to three plots, using a different controlled-release formulation-gelatin microcapsules, flakes of plastic laminates, or hollow fibers-in each plot. Disparlure concentrations in air were measured intermittently over a 34-day period after application. Measurements were made at four heights above ground: 0.3,2,5, and 10m. Wind speed and air temperature were measured concurrently. Disparlure was emitted from the microcapsules and fibers at very high levels for the first day or two; thereafter, all three formulations produced gradually decreasing aerial concentrations. After 32–34 days, concentrations in all plots ranged from 1.5% to 15.5% of those on the first day, although most of the pheromone remained in the formulations. Results suggest that the effective life of all three formulations for mating disruption by atmospheric permeation would be near 30 days under the test conditions, with fibers likely to have the shortest life. Concentrations varied diurnally throughout the experiment, with highest levels generally between 1400 and 2200 hr each day. Concentrations were highest at the 10-m height with microcapsules and at the 0.3- and 10-m heights with flakes, but were similar at all heights with fibers. Biological implications of the results are presented.
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    Analysis of field-weathered lures containing (E)-10-dodecen-1-yl acetate, a sex attractant for the pea moth,Cydia nigricana (F.) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 1049-1056 
    Details
    ISSN: 1573-1561
    Keywords: Pea moth ; Cydia nigricana (F.) ; Lepidoptera ; Olethreutidae ; sex attractant ; (E)-10-dodecen-1-yl acetate ; lure ; formulation ; monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Natural rubber stoppers treated with 1, 3, or 10 mg of (E)-10-dodecen-1-yl acetate, an attractant for male pea moth,Cydia nigricana (F.), were exposed in the field during the summer of 1978 and samples analyzed at intervals. The results for all three doses fitted well to first-order loss curves with half-lives of 63.5, 64.7, and 67.3 days, respectively. Thus, lures with an initial dose of 3 mg of the attractant retained approximately 1 mg after 3 months of field exposure; they should therefore maintain a constant level of attractiveness throughout this period because previous field studies showed that moths were equally responsive to fresh lures containing between 1 and 10 mg of the attractant. There was no loss of attractant during the preparation of lures or after 4 months at −15 ° C and only 13% was lost during 3 months of storage at room temperature.
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    URL: http://dx.doi.org/10.1007/BF00987626
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    Sex pheromone of the tomato looper,Plusia chalcites (ESP) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 1081-1088 
    Details
    ISSN: 1573-1561
    Keywords: Plusia chalcites ; Lepidoptera ; Noctuidae ; tomato looper ; sex pheromone identification ; glass WCOT capillary chromatography ; gas chromatography-mass spectrometry ; Z-7-dodencenyl acetate ; Z-9-tetra-decenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Z-7-Dodecenyl acetate (A) andZ-9-tetradecenyl acetate (B), in a 5∶1 ratio, have been identified as sex pheromone components of femalePlusia chalcites. In addition, small amounts of dodecyl acetate, tetradecyl acetate, hexadecyl acetate, andZ-9-dodecenyl acetate have been found in the abdominal tip extracts. This is the first known Plusiinae species which utilizes a two-component primary sex pheromone system. The most effective lure found in field experiments was a mixture of 1 mg A and 0.2 mg B absorbed on rubber septa.
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    Larval osmeterial secretions of the swallowtails (Papilio) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 1089-1113 
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    ISSN: 1573-1561
    Keywords: Osmeterial secretion ; Papilio larvae ; Lepidoptera ; monoterpene ; sesquiterpene ; aliphatic acid ; ester
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The larval osmeterial secretions of sixPapilio species examined displayed a remarkable qualitative change at the fourth larval ecdysis. The secretions of 4th (penultimate) instar larvae ofP. machaon, P. memnon, P. helenus, P. bianor, andP. maackii principally comprised mono- and/or sesquiterpenoids. The compounds identified from these species included α-pinene, sabinene, β-myrcene, limonene, β-phellandrene, (Z)-β-ocimene, (E)-β-ocimene, β-elemene, β-caryophyllene, (E)-β-farnesene, β-selinene, (E,E)-α-farnesene, germacrene-A, germacrene-B, caryophyllene oxide, methyl 3-hydroxy-n-butyrate, and acetic acid. In contrast, the secretion of 4th larval instar ofP. xuthus, although containing similar terpenic compounds, was accompanied by large amounts of aliphatic acids and their esters: isobutyric acid, 2-methylbutyric acid, methyl isobutyrate and methyl 2-methylbutyrate. On the other hand, the osmeterial secretions of 5th (last) instar larvae varied little in quality among the six species, and the identified compounds consisted of isobutyric acid, 2-methylbutyric acid, methyl isobutyrate, ethyl isobutyrate, methyl 2-methylbutyrate, ethyl 2-methylbutyrate, and isovaleric acid, the last of which was specific toP. bianor andP. maackii. The chemical alteration of osmeterial exudate synchronized at the final larval ecdysis with the larval morphological change (particularly in body coloration) that appears to be of defensive significance against predators.
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    On the persistence of disparlure in the human body (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 313-317 
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    ISSN: 1573-1561
    Keywords: Disparlure ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; pheromone persistence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Response of male gypsy moths from a sparse infestation in Italy to an observer apparently contaminated with disparlure at least two years previously is reported. Other examples of pheromone contamination of people or things are also recorded.
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    Comparing the effectiveness of sexual communication disruption in the oriental fruit moth (Grapholitha molesta) using different combinations and dosages of its pheromone blend (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 501-508 
    Details
    ISSN: 1573-1561
    Keywords: Disruption of communication ; Oriental fruit moth ; Grapholitha molesta ; Lepidoptera ; Tortricidae ; sex pheromone ; sex attractant ; (Z)-8-dodecenyl acetate ; (E)-8-dodecenyl acetate ; (Z)-8-dodecen-1-ol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The relative efficacy of disruptant blends comprised of different combinations of the Oriental fruit moth's pheromone components was determined in field tests. Disruption was evaluated by comparing male moth catch at synthetic and female-baited traps in disruptant and non-treatment areas. Three atmospheric dosages of a 8-dodecenyl acetate (93.5%Z∶6.5%E) blend, representing two successive 10-fold decreases in concentration (2.5 × 10−2 g/hectare/day to 2.5 × 10−4 g/hectare/day) were tested alone and in combination with an additional percentage of (Z)-8-dodecen-1-ol. Male moth orientation to traps was eliminated in plots exposed to the two highest binary acetate dosages. However, significantly more males were captured in synthetic-baited traps in the lowest acetate-alone treatment, indicating a diminution of disruption efficiency. In contrast, inclusion of (Z)-8-dodecen-1-ol in the disruptant blend effected essentially complete disruption of orientation at all concentrations tested. Mating success ofG. molesta pairs confined in small cages apparently was not affected by the presence of relatively high concentrations of the binary acetate and the acetate-alcohol blends. This suggests that habituation and/or adaptation of male response, at least for comparatively “close-range” behaviors, did not occur.
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    Seven-week persistence of an oviposition-deterrent pheromone (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 583-588 
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    ISSN: 1573-1561
    Keywords: Oviposition ; deterrent pheromone ; Pieris brassicae ; Lepidoptera ; Pieridae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Cabbage leaves sprayed with a water solution of the oviposition-deterrent pheromone ofPieris brassicae remain deterrent to ovipositing females for at least 14 days. Under laboratory conditions, the pheromone, when dried on a glass surface, retains activity for a period of at least 7 weeks. After 7 days under vacuum conditions, some pheromone is still present, indicating a low volatility and / or high stability of (an active fraction of) the pheromone. After 125 eggs are slowly rinsed with 300 ml water, they still release detectable quantities of the pheromone.
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    Effect of day length and light intensity on 2-tridecanone levels and resistance inLycopersicon hirsutum f.glabratum toManduca sexta (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 707-716 
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    ISSN: 1573-1561
    Keywords: Manduca sexta (L.) ; Lepidoptera ; Sphingidae ; Lycopersicon hirsutum f.glabratum ; day length ; light intensity ; 2-tridecanone ; glandular trichome ; resistance ; allelochemic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract First instarManduca sexta (L.) larvae confined on foliage fromLycopersicon hirsutum f.glabratum (accession PI 134417) plants grown under a long-day regime exhibited greater mortality than larvae on foliage from plants grown under a short-day regime. 2-Tridecanone, a toxin important in the insect resistance of PI 134417, was significantly more abundant in the foliage of plants grown under the long-than the short-day regimes. Light intensity influenced neither 2-tridecanone levels nor the expression of resistance. The density of glandular trichomes, which secrete 2-tridecanone, was influenced by an interaction between day length and light intensity.
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    Growth inhibitors in tomato (Lycopersicon) to tomato fruitworm (Heliothis zea) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 753-758 
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    ISSN: 1573-1561
    Keywords: Lycopersicon esculentum ; Lycopersicon hirsutum f. glabratum Heliothis zea ; Lepidoptera ; Noctuidae ; α-tomatine ; rutin ; chlorogenic acid ; caffeyl aldaric acid ; allelochemics ; tomato ; tomato fruitworm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Several compounds that retard the larval growth of the tomato fruitworm,Heliothis zea (Boddie) have been isolated and identified from tomato leaves,Lycopersicon esculentum Mill. The major allelochemics are α-tomatine (I), chlorogenic acid (II), rutin (III), and a new caffeyl derivative of an aldaric acid (IV). The isolation, analyses, and toxicity of these compounds toH. zea are presented.
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    Biological amplification for increasing electroantennogram discrimination between two female sex pheromones ofSpodoptera littoralis (Lepidoptera: Noctuidae) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 791-798 
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    ISSN: 1573-1561
    Keywords: Spodoptera littoralis ; Lepidoptera ; Noctuidae ; electroantennogram ; biological amplification ; pheromones ; Boarmia selenaria ; Geometridae ; Matsucoccus josephi ; Homoptera ; Coccoidea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract MaleSpodoptera littoralis stimulated by a mixture ofS. littoralis female sex pheromone with a positional isomer and by the pheromone alone, gave greatly amplified electroantennograms (EAGs) when the antennae were connected in series. The technique is described and examples are given of its use in screening extracts for EAG activity. Dose-response curves obtained with this technique are presented and discussed. Through biological amplification, EAG discrimination between the pheromones was increased.
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    Alleviation of α-tomatine-induced toxicity to the parasitoid,Hyposoter exiguae, by phytosterols in the diet of the host,Heliothis zea (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 927-946 
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    ISSN: 1573-1561
    Keywords: Host-plant resistance ; α-tomatine ; sterols ; sterol esters ; Lycopersicon esculentum ; Heliothis zea ; Lepidoptera ; Noctuidae ; parasitoids ; Hyposoter exiguae ; Hymenoptera ; Ichneumonidae ; secondary compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The solitary, endoparasitic ichneumonid,Hyposoter exiguae (Viereck) is a parasitoid of the tomato fruitworm,Heliothis zea (Boddie). However, the parasitoid is deleteriously affected by the tomato glycoalkaloid, α-tomatine, ingested from hosts (H. zea) fed on artificial diets or semipurified extracts of tomato plants that contained α-tomatine. α-Tomatine causes prolonged larval development; disruption or prevention of pupal eclosion; deformation of antennal, abdominal, and genital structures; and reduction in adult weight and longevity of the parasitoid. These toxic effects are exacerbated when the dietary dose of α-tomatine is increased from 12 μmol to 20 μmol/g dry wt of diet. However, the toxicity of α-tomatine is attenuated in parasitoids reared from hosts fed on artificial diets that contain equimolar or supramolar amounts of 3β-OH-sterols admixed with α-tomatine. Further, the toxicity of extracts from the foliage of different cultivars of tomatoes toH. exiguae is contingent upon the composite levels of α-tomatine and total phytosterol (free and esterified). Cultivars with a low total sterol-tomatine ratio are more toxic toH. exiguae. The possible mode of action of α-tomatine toxicity toH. exiguae by disruption of sterol metabolism is discussed.
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    Kairomones and their use for management of entomophagous insects (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 1057-1061 
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    ISSN: 1573-1561
    Keywords: Heliothis zea ; Lepidoptera ; Noctuidae ; Microplitis croceipes ; Hymenoptera ; Braconidae parasitoids ; kairomones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In laboratory experiments conducted to compare the kairomonal activity forMicroplitis croceipes (Cresson) of frass fromHeliothis zea (Boddie) larvae fed on different host plants,M. croceipes females responded to extracts of frass from larvae reared on cotton or soybeans but not on corn. The lack of response to frass from larvae reared on corn was shown to be a result of a lack of some appropriate chemical(s) in the corn.
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    Observations on cytoplasmic transport along ovarian nutritive tubes of polyphagous coleopterans (1981)
    Springer
    Cell & tissue research 220 (1981), S. 153-161 
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    ISSN: 1432-0878
    Keywords: Cytoplasmic transport ; Insect ovary ; Autoradiography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ovarioles of Coccinella and Tenebrio are shown to be telotrophic — a characteristic normally associated with hemipterans rather than coleopterans. They possess an anterior region of trophic cells and a chain of oocytes. The trophic cells are connected with the latter by a series of nutritive tubes, and autoradiography has shown that RNA is transported along the tubes to the oocytes. However, the system in these beetles differs markedly from that of hemipterans in that the nutritive tubes do not contain an extensive complement of aligned microtubules. The significance of this to both the mechanism and the selectivity of transport is discussed.
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    Electron microscopic and histochemical investigations of the atretic oocyte of Perca fluviatilis L. (Teleostei) (1981)
    Springer
    Cell & tissue research 220 (1981), S. 201-212 
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    ISSN: 1432-0878
    Keywords: Atretic oocyte ; Perca fluviatilis ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The regression of atretic oocytes in Perca fluviatilis was studied by histochemical, light and electron microscopic methods. The course of regression can be divided into three stages, the first two comprising the dissolution of the atretic oocyte and its phagocytosis by the granulosa cells of the follicular epithelium, and the third stage consisting of the dissolution of the granulosa cells themselves. The ultrastructure in all three stages shows only features related to phagocytosis and lysosome formation. In particular, there is no agranular endoplasmic reticulum formed within the phagocytically active granulosa cells, nor is there any 3β-hydroxysteroid dehydrogenase activity (3β-HSD). Large yellow-orange pigments, formed during the third stage of regression, are ascribed to a relative deficiency of lysosomes in lipid digestion, and do not result from a preceeding steroid-synthesising phase as in mammalian corpora lutea. Thus, the atretic oocyte of P. fluviatilis is considered not to give rise to a corpus luteum formation with endocrine function, but merely represents a degenerative structure.
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    Sex pheromone components isolated from china corn borer,Ostrinia furnacalis guenée (lepidoptera: Pyralidae), (E)- and (Z)-12-tetradecenyl acetates (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 841-851 
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    ISSN: 1573-1561
    Keywords: Lepidoptera ; Pyralidae ; Ostrinia furnacalis ; sex pheromone ; corn borer ; (E)-12-tetradecenyl acetate ; (Z)-12-tetradecenyl acetate ; tetradecyl acetate ; gas chromatography-mass spectrometry ; selected ion monitoring technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The sex pheromone components from the corn borer spreading widely in China,Ostrinia furnacalis Guenée, have been identified as (E)-and (Z)-12-tetradecenyl acetates (E andZ12–14∶ Ac). The ratio ofE isomer toZ isomer was 53 ∶ 47. Traps containing 1 × 10−7−1 × 10−5 g of these compounds captured more males than did live females or their tip extract (3–6 female equivalents). Tetradecyl acetate (14 ∶ Ac) was also identified in the tip extract. Its quantity was about 1.8 times the sum of the other two isomers. However, including this compound in its natural ratio in pheromone traps resulted in a decrease in trap catches (P〈 0.05).
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    Identification of a sex pheromone ofHeliothis subflexa (GN.) (Lepidoptera: Noctuidae) and field trapping studies using different blends of components (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 1011-1022 
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    ISSN: 1573-1561
    Keywords: Sex pheromone ; Heliothis subflexa ; Lepidoptera ; Noctuidae ; reproductive behavior ; field trapping ; capillary chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Eight compounds were isolated from the sex pheromone gland ofHeliothis subflexa (Gn.) and identified as hexadecanal, (Z)-9-hexadecenal, (Z)-11-hexadecenal, (Z)-7-hexadecen-1-ol acetate, (Z)-9-hexadecen-1-ol acetate, (Z)-11-hexadecen-1-ol acetate, (Z)-9-hexadecen-1-ol, and (Z)-11-hexadecen-1-ol. Although the whole blend was found to be an effective male attractant, the deletion of alcohols from the blend increased trap captures considerably. Further, although the binary mixture of (Z)-9-hexadecenal and (Z)-11-hexadecenal caught some maleH. subflexa, significant increases in captures were noted when the three acetate components were included in the blend.
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    Disintegration of spermatozoa in the infundibular sperm-host glands of the fowl (1981)
    Springer
    Cell & tissue research 214 (1981), S. 81-87 
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    ISSN: 1432-0878
    Keywords: Sperm-host glands ; Spermatozoa ; Fowl ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The disintegration of spermatozoa in the infundibular sperm-host glands of the fowl was investigated by electron microscopy. After the 15th day following artificial insemination, secretory granules in the epithelial cells of the sperm-host glands increase in number and size, and subsequently the contents of the granules are released into the glandular lumen, so that the electron density of the lumen increases. At this stage, spermatozoa stored in the glands begin to undergo degenerative changes starting from the head. The heads become distended and chromatin of the nucleus begins to disperse as small masses, simultaneously with the destruction of the acrosome. As the dispersion of chromatin progresses, mitochondria of the middle piece become distended and irregular in shape, and then disintegrate. At the last stage, most of the organelles have disappeared, but the fibrous sheath and axial-filament complex are still identified.
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    Fate of spermatozoa that do not participate in fertilization in the domestic fowl (1981)
    Springer
    Cell & tissue research 214 (1981), S. 89-95 
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    ISSN: 1432-0878
    Keywords: Spermatozoa ; Vitelline membrane ; Macrophage ; Fowl ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fate of spermatozoa that do not participate in fertilization was investigated by electron microscopy. After artificial insemination, we observed several spermatozoa between the fibers of the outer layer of the vitelline membrane of the ovum. One or more spermatozoa were also found in a phagocytic vesicle of macrophages located in the intercellular space of the mucosal epithelium of the infundibulum or in the outer layer of the vitelline membrane. From these observations, we assume that the superfluous spermatozoa in the lumen of the anterior part of the oviduct might be removed by inclusion into the outer layer of the vitelline membrane and by phagocytosis by macrophages.
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    Hepatocyte differentiation during early fetal development in the rat (1981)
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    Cell & tissue research 215 (1981), S. 133-142 
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    ISSN: 1432-0878
    Keywords: Rat fetus ; Hepatocyte differentiation ; Ultrastructure ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rat hepatocyte differentiation between day 12 and 19 of fetal life was studied by electron microscopy. The cytoplasmic structures involved in synthetic and secretory function, i.e., rough endoplasmic reticulum and Golgi apparatus, appear to be the first to differentiate, and their development is probably related to the secretion of different kinds of plasma proteins. The cytoplasmic organelles involved in other hepatic functions develop later: lysosomes from day 15, peroxysomes, glycogen rosettes and smooth endoplasmic reticulum still later. However, the morphological differentiation of bile canaliculi begins from day 12.
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    Fluid transport and dimensions of epithelial cells and intercellular spaces in frog gallbladder (1981)
    Springer
    Cell & tissue research 215 (1981), S. 223-247 
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    ISSN: 1432-0878
    Keywords: Active transport ; Supravital microscopy ; Electron microscopy ; Preparative cell shrinkage ; Frog gallbladder
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Morphologic findings of widely dilated intercellular spaces in fluid transporting epithelia have been claimed as evidence for the existence of an epithelial compartment in which the coupling between solute and water fluxes takes place. The validity of using epithelial geometry in sectioned material as an argument can be questioned. The present report describes the morphological appearance of frog gallbladder epithelium — normal and ouabain-treated — in the living state in vitro and after fixation, dehydration and embedding. Gallbladder segments were photographed in the living state and at the end of each step of the preparative procedure. Direct observations of whole-mounted gallbladder segments were carried out, taking advantage of the possibility of optical sectioning and high resolution by Nomarski-microscopy. The same specimens were then sectioned and examined by conventional light and electron microscopy. The observations were quantitated and showed that the epithelial cells of normal and ouabain-treated gallbladders experienced an average linear shrinkage down to 70% of their length in Ringer's solution, which corresponds to a volume shrinkage down to 35%. Moreover, dilated lateral intercellular spaces appeared during the dehydration and embedding procedure in normal but only very moderately or not at all in ouabain-treated gallbladder specimens.
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    Ultrastructural study of the postembryonic development of the neural lamella of Galleria mellonella L. (Lepidoptera) (1981)
    Springer
    Cell & tissue research 217 (1981), S. 425-433 
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    ISSN: 1432-0878
    Keywords: Lepidoptera ; Neural lamella ; Postembryonic development ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The neural lamella encapsulating the brain of the wax moth Galleria mellonella develops from a very thin (80–120 nm) layer in the first larval instar, resembling the basal lamina, to a thick (1–4 μm) sheath composed of two zones in the seventh (last) instar. After its breakdown at the time of larval-pupal ecdysis the neural lamella is reconstructed in the pupa, 2–3 days before pupaladult ecdysis. The cells of the perineurium seem to be responsible for the formation of the neural lamella, both in the larva and pupa, even though its ultrastructure differs at these stages.
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    The structure and distribution of satellite cells of cardiac muscles in decapod crustaceans (1981)
    Springer
    Cell & tissue research 219 (1981), S. 69-83 
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    ISSN: 1432-0878
    Keywords: Satellite cells ; Cardiac muscle ; Decapod crustaceans ; Distribution ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure and distribution of satellite cells of cardiac muscles were examined in twenty-one species of animals chosen from each tribe within the order Decapoda (Arthropoda, Crustacea). The satellite cells were found in all animals observed. Most of them are morphologically identical with those described in different striated muscles of other species, but some cells have unusual features. The decapod satellite cell occasionally lies right over the region corresponding to the intercalated disc between the apposed cardiac muscle cells. The cell sends cytoplasmic processes into the adjacent muscle cells, enabling the plasma membrane to make close contact with the cleft opening of the intercalated disc, and with the myofibril at the level of the Z-line. Another characteristic feature is the presence of “paired” cells. Such cells are clearly separated from each other over most of the contact area by the respective plasma membranes, which are smooth in appearance and devoid of specialized regions. The significance of the presence of satellite cells in decapod cardiac muscle and its possible role are discussed and compared with those described for other species.
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    Immuno-electron-microscopic identification of O-antigen-bearing oligodendroglial cells in vitro (1981)
    Springer
    Cell & tissue research 219 (1981), S. 313-325 
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    ISSN: 1432-0878
    Keywords: Oligodendroglia ; Cell surface antigens ; Electron microscopy ; Immunoperoxidase ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Monoclonal antibodies to cell-surface antigens of oligodendrocytes (Sommer and Schachner 1980; Schachner et al. 1980) were used to identify this cell type by immuno-electron microscopy in monolayer cultures of fetal and early postnatal mouse cerebellum. The ultrastructural features of antigen-positive cells confirm that they are immature and mature oligodendrocytes, but not neurons, astrocytes or fibroblasts or fibroblast-like cells. Type I oligodendrocytes are the immature ones with a relatively large amount of moderately electron-lucent cytoplasm, clusters of ribosomes and complex networks of rough endoplasmic reticulum. Large numbers of mitochondria and microtubules, but not intermediate-sized filaments are seen in these cells. They comprise more than 90% of all 0-antigen-positive cells. Type II cells comprise only approximately 5% of all 0-antigen-positive cells. They are characterized by a limited amount of electron-dense cytoplasm, which appears more compact and granular than in type I cells. The rough endoplasmic reticulum is distributed evenly throughout the cytoplasm. Microtubules and mitochondria are present, but more difficult to distinguish due to the compactness of the cytoplasm. Type II cells display the more mature ultrastructural features of oligodendrocytes.
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    URL: http://dx.doi.org/10.1007/BF00210151
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    Muscle cells in a nerve trunk of a frog muscle (1981)
    Springer
    Cell & tissue research 219 (1981), S. 433-436 
    Details
    ISSN: 1432-0878
    Keywords: Electron microscopy ; Frog ; Cutaneus pectoris muscle ; Nerve ; Muscle spindle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Three muscle fibers were identified by electron microscopy within a nerve of a frog muscle. They resembled extrafusal muscle fibers but were located in an endoneurial rather than in an endomysial compartment. To call these endoneurial muscle fibers the obvious continuation of extrafusal fibers of a muscle spindle is certainly unwarranted; to label these fibers ectopic and to let the matter rest there is probably an understatement of sorts.
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    URL: http://dx.doi.org/10.1007/BF00210161
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    Intracellular polymerized haemocyanin in the branchial gland of a cephalopod (1981)
    Springer
    Cell & tissue research 220 (1981), S. 435-438 
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    ISSN: 1432-0878
    Keywords: Haemocyanin ; Branchial gland ; Electron microscopy ; Eledone moschata ; Cephalopoda
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Polymerized haemocyanin molecules have been identified as rings, about 25 nm in diameter, forming linear arrays within cytoplasmic vesicles, close to the nucleus. They were observed by transmission electron microscopy in the polygonal cells of the branchial gland of Eledone moschata Lamarck. These observations confirm previous data suggesting that haemocyanin is synthetized in the branchial gland cells of Octopoda.
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    URL: http://dx.doi.org/10.1007/BF00210520
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    Ultrastructural localization of endogenous peroxidase in the lower respiratory tract of the guinea pig (1981)
    Springer
    Cell & tissue research 214 (1981), S. 407-415 
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    ISSN: 1432-0878
    Keywords: Endogenous peroxidase ; Trachea ; Lung ; Electron microscopy ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Endogenous peroxidase activity was demonstrated by cytochemistry in mucous cells of the submucosal glands and tracheobronchial epithelium of guinea pigs. It is localized in the nuclear envelope, in cisternae of rough endoplasmic reticulum, and in secretory granules. It was not seen in Golgi saccules or in the airway lumen. By contrast, all epithelial cells within the lung including mucous (goblet) cells lack activity. Reaction product is also absent from alveolar macrophages and mast cells. The appearance of peroxidase in mucous cells is age-related. No activity was seen at 1.5ms of age. A few mucous cells were positive at 2.5 and 3 ms while the proportion of positive cells increased substantially up to 7 ms. Thus, the age of guinea pigs in HRP transport studies must receive careful consideration in order to avoid misinterpretation of results. The function of mucous cell peroxidase is unknown. The results of this study suggest that it is secreted. Whether it plays a significant role in lung defense through its well documented anti-infectious properties remains to be determined.
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    URL: http://dx.doi.org/10.1007/BF00249221
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    A possible pathway of chitin synthesis as revealed by electron microscopy in Tetranychus urticae (Acari, Tetranychidae) (1981)
    Springer
    Cell & tissue research 214 (1981), S. 443-448 
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    ISSN: 1432-0878
    Keywords: Chitin synthesis ; Electron microscopy ; Acari ; Tetranychus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two chitin synthesising systems in Tetranychus urticae are described: one chitosomal system located in the oocytes where spatial and temporal distances are large, and one membrane bound system located in the hypodermis. Similarity of mechanisms of chitin synthesis in animals and plants is suggested.
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    URL: http://dx.doi.org/10.1007/BF00249224
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    Final stages of erythrophagocytosis in the sheep placenta (1981)
    Springer
    Cell & tissue research 214 (1981), S. 501-518 
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    ISSN: 1432-0878
    Keywords: Placenta (sheep) ; Cell phagocytosis ; Hemoglobin-derived pigments ; Residual bodies ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In trophoblastic epithelial cells of the sheep placenta the final stages of erythrocyte breakdown within the lysosomal apparatus were studied at the ultrastructural level. As a result of hemoglobin digestion lysosomes containing hemoglobin-derived pigments (HDP) were formed. The HDP-lysosomes were acid phosphatase-positive, highly electron-dense bodies of round to irregular shape containing whorled membranous formations. The accumulation of these lysosomes in epithelial cells led to fusion resulting in the formation of conglomerates. At the end of the gestation period the amount of HD Plysosomes and their conglomerates markedly increased. In addition to erythrocytes the trophoblastic epithelial cells in the erythrophagocytic regions phagocytosed maternal leukocytes and neighbouring epithelial cells and giant cells. By gradual accumulation of HDP-lysosomes and remnants of phagocytosed cells, highly electron-dense acid phosphatase-positive residual bodies of variable appearance were formed within the epithelial cells. At the end of pregnancy the spaces between juxtaposed villi of the trophoblastic epithelium in the erythrophagocytic zones were occluded by apposition of the epithelial cells. In these occluded regions an increase in highly electron-dense large-sized residual bodies (15–22 μm of dimension) occurred as a result of multiple cell phagocytosis in combination with fusion. In these residual bodies the numerous incorporated HDP-lysosomes and the remnants of phagocytosed cells could still be recognized.
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    URL: http://dx.doi.org/10.1007/BF00233491
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    Disaccharidase-deficient animals have normal ultrastructure of intestinal brush border membranes (1981)
    Springer
    Cell & tissue research 220 (1981), S. 555-559 
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    ISSN: 1432-0878
    Keywords: Disaccharidases ; Intestinal brush-border membranes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The intestinal disaccharidases, lactase, sucrase-isomaltase complex, and glucoamylase are proteins intimately associated with the brush-border membrane of the epithelial cell. These three enzyme activities are found in the intestine of the adult rat; lactase and glucoamylase activities are primarily associated with the intestine of the infant rat. Only glucoamylase and isomaltase activities are detected in the intestine of the California sea lion, Zalophus californianus. The activities of these enzymes are detected only in villus cells, and not in crypt cells. We have carried out electron microscopic studies of negatively stained brush-border preparations of intestinal crypt and villus cells; from the intestine of the 10-day-old rat and from that of the California sea lion. The density of the knob-like structures protruding from the brush-border membranes was not significantly different in any of these preparations. The diameter of the knobs on the preparations from crypt cells was smaller than the diameters of the knobs found on membranes prepared from the other sources. These data are discussed in terms of the relationship between the presence of knob structures and disaccharidase activities associated with the brush-border membranes.
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    URL: http://dx.doi.org/10.1007/BF00216759
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    Ruthenium red staining and tannic acid fixation of dental basement membrane (1981)
    Springer
    Cell & tissue research 220 (1981), S. 589-597 
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    ISSN: 1432-0878
    Keywords: Tooth basement membrane ; Ruthenium red ; Tannic acid ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ruthenium red staining and tannic acid fixation were used to analyse the fine structure of embryonic mouse dental basement membrane in intact first mandibular molars or in EDTA-isolated dental papillae. Preameloblasts are separated from extracellular matrix proper by a basal lamina that contains regularly arranged proteoglycan granules of about 10 nm in diameter. This distribution pattern is particularly evident in the inner and outer lamina rara of the basal lamina associated with EDTA-isolated dental papillae. The plasmalemma of preameloblasts demonstrates electron dense plaques on the inner leaflet. Ruthenium red positive granules (50 nm in diameter) coat non-striated and striated fibrils of the matrix. Hyaluronidase treatment digested the ruthenium red positive granules. Tannic acid fixation allowed the demonstration of filaments within the lamina rara interna, connecting the lamina densa with plasmalemma of preameloblasts. These observations are discussed in the context of the terminal differentiation of odontoblasts.
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    URL: http://dx.doi.org/10.1007/BF00216762
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    Monoamines in the brain of the lancelet, Branchiostoma lanceolatum (1981)
    Springer
    Cell & tissue research 221 (1981), S. 245-256 
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    ISSN: 1432-0878
    Keywords: Monoamines ; Fluorescence histochemistry ; Electron microscopy ; Branchiostoma lanceolatum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Three types of monoamine-containing neurones and fibres can be discriminated in the brain of the lancelet. Two types of elongated cerebrospinal fluid-contacting neurones, located in the ventral and the dorsolateral part of the brain, exhibit formaldehyde-induced catecholamine fluorescence. These neurones contain dense-core vesicles 75–100 nm in diameter. Their apical portion possesses cilia, displaying a 9×2+2 arrangement of their internal tubuli, and projecting into the ventricle. Basal processes from the ventrally situated perikarya abut upon the meninx and may discharge their catecholamines into the circulatory system. Fibres exhibiting catecholamine fluorescence originate from the dorsolaterally situated perikarya and run ventrocaudally to the neuropil, where they form numerous swellings of the bouton en passant type. A third type of perikarya in the posterior part of the brain displays specific green fluorescence. Further, neurones characterized by a specific yellow fluorescence are present in the anterior part of the brain and the anterior part of the neural tube. The rapid photodecomposition of the latter fluorophore indicates that these cells contain an indolamine.
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    URL: http://dx.doi.org/10.1007/BF00216729
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    Stage-dependent variations in volume density and size of Sertoli cell vesicles in the rat testis (1981)
    Springer
    Cell & tissue research 221 (1981), S. 311-320 
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    ISSN: 1432-0878
    Keywords: Sertoli cell (rat) ; Testis ; Vesicles ; Morphometry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Membrane-bounded spherical vesicles found in rat Sertoli cells have been examined quantitatively during the cycle of the seminiferous epithelium. Most of the vesicles were localized to the basal and columnar portions of the Sertoli cell cytoplasm. The thin lateral projections of the Sertoli cells contained very few vesicles. Morphometric analysis of the basal portion of the Sertoli cell cytoplasm revealed that the volume density (V v ) of the vesicles changed markedly during the cycle. The V v was at its minimum (0.036) at stage VII and maximum (0.117) at stages XI-I. The vesicles were also smaller at stage VII compared to the vesicles at stages IX-V. The stage-dependent difference in the size of the vesicles was found both in the basal and the columnar portions of the Sertoli cells. At stage VII some of the vesicles appeared to be elongated much like the tubular elements of the smooth endoplasmic reticulum (SER) from which they are probably derived. The stage-dependent differences in volume density and size of the Sertoli cell vesicles may be related to cyclic biochemical variations in the Sertoli cells, and are further indications of a variation in Sertoli cell function during the cycle of the seminiferous epithelium. Whether or not this is due to an “internal” cycle of the Sertoli cell or to influences from adjacent germ cells remains to be determined.
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    URL: http://dx.doi.org/10.1007/BF00216735
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    Morphology of regenerated spinal cord in Sternarchus albifrons (1981)
    Springer
    Cell & tissue research 219 (1981), S. 1-8 
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    ISSN: 1432-0878
    Keywords: Regeneration ; Spinal cord ; Neurons ; Ependyma ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The tail of the gymnotid Sternarchus albifrons, including the spinal cord, regenerates following amputation. Regenerated spinal cord shows a rostro-caudal gradient of differentiation. Cross sections of the most distal regenerated cord show radially enlarged ependymal cells, relatively undifferentiated cells, and numerous blood vessels. More anterior sections contain well differentiated electromotor neurons, glial cells, and myelinated axons. The number of electromotor-neuron cell bodies in cross sections of regenerated spinal cord is three to six times the number in nonregenerated cord. Distinct tracts of axons, easily identifiable in normal cord, are not distinguishable in cross sections of regenerated cord. Some reorganization of the spinal cord also appears to take place anterior to the site of transection. Individual electromotor neurons in the regenerated spinal cord have morphologies largely similar to those of normal electrocytes, i.e., cell bodies are rounded, lack dendrites, have synapses characterized by gap junctions with presynaptic axons, and lack an unmyelinated initial segment. The presence of electromotor neurons with normal morphology in regenerated spinal cord correlates with the re-establishment of relatively normal electrocyte axonSchwann cell relationships in the regenerating electric organ of this sternarchid.
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    URL: http://dx.doi.org/10.1007/BF00210014
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    Myelinated Herring bodies in the posterior part of the median eminence of the mouse (1981)
    Springer
    Cell & tissue research 219 (1981), S. 209-211 
    Details
    ISSN: 1432-0878
    Keywords: Herring bodies ; Median eminence ; Myelinated axons ; Mouse ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The posterior part of the median eminence of the albino mouse (CF # 1-JCL) contains a cluster of myelinated axons beneath the tanycyte layer. Among them, small Herring bodies surrounded by myelin sheaths are revealed by electron microscopy. These structures contain electron-dense bodies, lamellar bodies, autophagic bodies, autophagic vacuoles, and neurofilaments. A few neurosecretory granules and mitochondria are also present. Some myelinated axons contain mostly accumulated neurosecretory granules.
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    URL: http://dx.doi.org/10.1007/BF00210029
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    Morphometric parameters of the midgut cells of Aedes aegypti L. (Insecta, Diptera) under various conditions (1981)
    Springer
    Cell & tissue research 219 (1981), S. 619-627 
    Details
    ISSN: 1432-0878
    Keywords: Mosquito strains ; Blood digestion ; Serum digestion ; Proteolytic activity ; Ribosomes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Frühere morphometrische und biochemische Untersuchungen erbrachten teilweise unterschiedliche Resultate betreffend Verteilung freier und membrangebundener Ribosomen in Mitteldarmzellen von Aedes aegypti. In der vorliegenden Arbeit wurde morphometrisch untersucht, ob diese Unterschiede bedingt waren durch die Verwendung verschiedener Mückenstämme, unterschiedlichen Futters und verschiedener Narkosemethoden durch die beiden Arbeitsgruppen, oder ob methodische Einflüsse dafür verantwortlich waren. Die meisten Zellparameter im Magenepithel von A. aegypti, Stamm „Rockefeller”, wie auch ihre Änderungen während der Verdauung eines Blutmahls, entsprachen den für einen andern Aedes-Stamm (Segemaganga, Hecker und Rudin 1979) gemessenen Werten und stimmten im allgemeinen mit denjenigen für Anopheles stephensi (Hecker 1978) überein. Die proteolytische Aktivität gegen Casein war bei beiden Stämmen gleich mit einem Aktivitäts-Maximum um 30h nach Blutmahl. Bei der Verdauung von menschlichem Serum konnte keine Zunahme des Verhältnisses von membrangebundenen zu freien Ribosomen, keine signifikante Oberflächenvergrößerung des rauhen endoplasmatischen Retikulums und keine signifikante Erhöhung der Zahl gebundener und freier Ribosomen gemessen werden. Die Proteaseaktivität war deutlich schwächer als während der Verdauung von Blut. Betäubung der Mücken vor der Sektion mit Aether oder durch Schütteln in Reagenzgläsern ergab im Vergleich keinen signifikanten Einfluß auf die Zellparameter von Zuckerwasser-gefütterten Weibchen, die drei Tage nach dem Schlüpfen untersucht wurden. Unterschiede in den Ribosomenparametern, die mit morphometrischen Methoden (Hecker und Rudin 1979) einerseits und biochemischen (Gander et al. 1980) andererseits untersucht wurden, konnten nur teilweise durch die Wahl unterschiedlichen Futters für die Mücken durch die beiden Arbeitsgruppen erklärt werden. Es müssen zusätzlich methodische Einflüsse für diese Unterschiede verantwortlich sein.
    Notes: Summary Previous morphometric or biochemical investigations have yielded different data on the distribution of free and membrane-bound ribosomes in midgut cells of Aedes aegypti. In the present paper ribosomal distribution has been morphometrically analysed to determine whether different mosquito strains, different food and different narcosis used in these previous studies, and/or methodological errors, could account for the different results. Most of the cellular parameters in the stomach epithelium of female A. aegypti, strain Rockefeller, and their changes during blood digestion, are comparable to those measured for another Aedes strain (Segemaganga, Hecker and Rudin 1979), and are generally similar to those of Anopheles stephensi (Hecker 1978). Proteolytic activity against casein is similar for both Aedes strains with a maximum activity being registered around 30 h after a blood meal. During digestion of human serum there is no increase in the ratio of membranebound to free ribosomes, and no significant increase in the surface area of the rough endoplasmic reticulum or of the number of bound or free ribosomes. Proteolytic activity is distinctly lower than during blood digestion. Immobilization of mosquitoes prior to dissection by ether narcosis or by shaking in a test tube has no significant influence on cellular parameters in females fed on sugar solution and investigated 3days after emergence. It is concluded that the differences in ribosomal parameters previously obtained by morphometrical (Hecker and Rudin 1979) and biochemical (Gander et al. 1980) methods, can only partly be explained by the selection of different food for the mosquitoes, and must also have been caused by methodological inadequacies.
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    URL: http://dx.doi.org/10.1007/BF00209999
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    Reversible changes in nuclear and cell surface topography in cells exposed to collagenase and EDTA (1981)
    Springer
    Cell & tissue research 220 (1981), S. 51-60 
    Details
    ISSN: 1432-0878
    Keywords: Cultured cells ; Detachment procedures ; Nuclear and cell surface ; Cytoskeleton ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rabbit auricular chondrocytes, SIRC cells, human fibroblasts, and HeLa cells were cultivated in vitro and the fine structural effects of various detachment procedures studied. Treatment with collagenase, trypsin, and trypsin-EDTA caused scalloping of the nuclear envelope, accumulation of phagolysosomes, and an increase in the number of cell surface extensions. Collagenase-EDTA evoked a marked deformation of the nuclei with formation of numerous deep indentations and a redistribution of heterochromatin. Similarly, the cell surface became extensively folded and the vacuolation of the cytoplasm was further increased. These changes were reversible and within 24 h the cells had regained a normal structure. In all cases, chondrocytes and SIRC cells were most prominently affected, whereas fibroblasts and HeLa cells were only slightly changed. Treatment of chondrocytes with colchicine or cytochalasin B did not produce any effects of the type mentioned above. Neither did treatment with the drugs before and during detachment with collagenase-EDTA prevent the structural modification of the cells. It therefore seems unlikely that micro tubules and micro filaments are essential for this process. The structural changes occurring during detachment of cells could represent an adoptive mechanism for disposal of excessive membrane in connection with transition from a flattened to a rounded shape.
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    URL: http://dx.doi.org/10.1007/BF00209965
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