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  • Articles  (1,758)
  • General Chemistry  (1,718)
  • Rat  (40)
  • 1970-1974  (1,061)
  • 1950-1954
  • 1910-1914  (697)
  • 1972  (1,061)
  • 1912  (697)
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  • Articles  (1,758)
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  • 1970-1974  (1,061)
  • 1950-1954
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  • 1
    Electronic Resource
    Electronic Resource
    Rate of normal longitudinal bone growth in the rat (1972)
    Springer
    Calcified tissue international 10 (1972), S. 238-251 
    Details
    ISSN: 1432-0827
    Keywords: Growth ; Bone ; Rat ; Tetracycline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé La vitesse de croissance normale en longueur de la métaphyse proximale du tibia est déterminée chez le rat Sprague-Dawley entre les âges de 20 et 100 jours, en utilisant la méthode à la tétracycline. Le taux de croissance ne varie que légèrement dans les groupes d'âges différents. Il est plus élevé chez les animaux jeunes et décroit considérablement en fonction de l'augmentation de l'âge. Les rats mâles présentent une croissance plus élevée que les femelles. Cette étude a pour but de mettre au point une méthode permettant de déterminer les facteurs expérimentaux, liés à la croissance en longueur du rat.
    Abstract: Zusammenfassung Die normale Längenwachstums-Geschwindigkeit der proximalen Wachstumsplatte der Tibia wurde bei Sprague-Dawley-Ratten in einem Alter zwischen 20 und 100 Tagen mittels der Tetracyclinmethode gemessen. Die Wachstumsgeschwindigkeit variierte nur wenig innerhalb der einzelnen Altersgruppen. Die Geschwindigkeit war bei jungen Tieren am höchsten und nahm mit zunehmendem Alter beträchtlich ab. Männliche Ratten wuchsen schneller als weibliche. Diese Arbeit dient als Grundlage, um die experimentelle Beeinflussung des Längenwachstums der Ratte abschätzen zu können.
    Notes: Abstract The rate of normal growth in length from the proximal growth plate of the tibia in the Sprague-Dawley rat was measured between 20 and 100 days of age using the tetracycline method. The growth rate varied only slightly within different age groups. The rate was highest in young animals and decreased considerably with increasing age. Male rats grew faster than female. This study is intended to provide a base for an evaluation of experimental influence on the growth in length of the rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02012553
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  • 2
    Electronic Resource
    Electronic Resource
    Immersion and perfusion fixed rat adrenal medulla: The problem of mixed cells, clear cells, and the mode of secretion (1972)
    Springer
    Cell & tissue research 126 (1972), S. 261-277 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal medulla ; Rat ; Cell types ; Secretion ; Influence of fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Nebennierenmark der Ratte wurde nach Anwendung verschiedener Fixationsmethoden untersucht. Nach Immersionsfixation mit Glutaraldehyd oder Osmiumtetroxyd finden sich in der Nebenniere sog. Mischzellen, helle Zellen, syncytiale oder plasmodiale Zellen, die von den Untersuchern als Artefaktbildungen angesehen werden. In allen Proben nach Perfusionsfixation wurden solche Artefaktbildungen niemals beobachtet. Bei Immersionsfixation wurde eine enge Beziehung zwischen Sekretgranula und Cytoplasmamembran beobachtet, was bei Perfusionsfixation selten der Fall war. Die heutigen Theorien über den Sekretionsmechanismus bei Nebennierenmarkzellen werden aufgrund der vorgelegten Ergebnisse diskutiert. Die Arbeit zeigt die wesentlichen Vorteile der Anwendung der Perfusionsfixation für die Untersuchung der Nebenniere.
    Notes: Summary The adrenal medulla of the rat was studied utilizing various methods of fixation. In adrenal medulla specimens after immersion fixation either with glutaraldehyde or osmium tetroxide, elements such as mixed, clear, syncytial, or plasmodial cells, believed to be of artifactual origin, are observed in all of this material examined. These elements are absent in the specimens prepared by perfusion fixation. In specimens prepared by immersion fixation, secretory granules are found in close proximity to the plasma membrane; this localization is infrequent after perfusion fixation. Current theories of the mechanism of secretion of adrenal medullary hormones are discussed on the basis of our results. This investigation demonstrates the advantage and necessity of perfusion fixation in the study of the adrenal medulla.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307220
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  • 3
    Electronic Resource
    Electronic Resource
    The fine structure of macrophages in the enamel organ, with special reference to the microtubular system (1972)
    Springer
    Cell & tissue research 126 (1972), S. 466-482 
    Details
    ISSN: 1432-0878
    Keywords: Macrophages ; Microtubules ; Enamel organ ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the enamel organ of rat incisors macrophages are present in the zone of matrix formation, the transitional zone, the enamel maturation and pigmentation zone. The macrophages accumulate adjacent to redifferentiating amelocytes in the transitional zone. The macrophages phagocytize fragments of disintegrating amelocytes. In addition to the well known complement of organelles the macrophages present an elaborated microtubular system, scattered, thick filaments, a cortical feltwork of thin filaments, and spherical nuclear bodies. The microtubules emanate from “attached” and free pericentriolar satellites and radiate aster-like towards the cell surface or into pseudopods or curve along the nuclear surface for long distances, often related to nuclear constrictions. It is suggested that the microtubular system plays a prominent role in directional movement of the macrophages. The cortical filaments, if contractile, may create the cytoplasmic flow necessary for the cell motility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306907
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  • 4
    Electronic Resource
    Electronic Resource
    An electron microscopic study of the normal synaptic relationships and early degenerative changes in the rat olfactory tubercle (1972)
    Springer
    Cell & tissue research 127 (1972), S. 462-482 
    Details
    ISSN: 1432-0878
    Keywords: Olfactory tubercle ; Rat ; Degeneration ; Synapse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The olfactory tubercle of the rat was studied by electron microscopy both in the normal and after ipsilateral olfactory bulb ablation at survival times of from 14 hours to seven days. Particular emphasis was placed on synaptic structures and their changes following the lesion. Normal synapses are similar to those described in previous studies and presynaptic profiles are of at least three types. Types-A and -B contain round vesicles and form asymmetrical contacts and type-C profiles contain flattened vesicles and form symmetrical contacts. There appear to be two major types of degenerative changes. The electron-lucent type predominates at early survival times and is seen first at 14 hours. These profiles show an early reduction in numbers of vesicles with mitochondrial swelling followed by shrinkage of the profile. These profiles become increasingly electron-dense at later survival times. The second major type of degenerating profile is initially electron-dense. The earliest changes in these profiles are an increased axoplasmic density and increased microtubular density and clumping without apparent loss of vesicles. These profiles also become progressively more electron-dense at longer survival times. The observations are discussed in relation to previous reports.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306866
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  • 5
    Electronic Resource
    Electronic Resource
    Intercellular bridges and division patterns of rat spermatogonia (1972)
    Springer
    Cell & tissue research 127 (1972), S. 201-208 
    Details
    ISSN: 1432-0878
    Keywords: Spermatogenesis ; Rat ; Mitosis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pattern of intercellular cytoplasmic bridges between rat spermatogonia and between spermatocytes is illustrated from electron microscopy of serial sections. Clones, or syncytia, containing as many as 22 connected spermatogonia and as many as 74 connected spermatocytes were observed. The absence of closed rings of cells agrees with the observation that intercellular bridges are the result of incomplete cell division, rather than cell fusion. The bridges thus are a record of spermatogonial divisions within a clone. In early spermatogonial generations there is a predominantly linear arrangement. The groups of spermatocytes have more side branches. From the presence of synaptonemal complexes it is concluded that the connected spermatocytes of a given clone are in about the same developmental stage. The pattern of intercellular bridges indicates, however, that not all nuclei in a clone undergo mitosis in the same cycle. The connected cells of a clone are therefore not all of the same generation. From unconnected bridges it is assumed that new clones originate from single cells or groups of spermatogonia which separate from an existing clone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306802
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  • 6
    Electronic Resource
    Electronic Resource
    Contractility and structure of adult rat seminiferous tubules in organ culture (1972)
    Springer
    Cell & tissue research 130 (1972), S. 171-179 
    Details
    ISSN: 1432-0878
    Keywords: Tubuli seminiferi ; Rat ; Structure ; Contractility ; Organ culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Isolated pieces of seminiferous tubules of adult rats were grown in organ culture for up to 8 weeks in Petri dishes on the surface of nutrient agar. The medium consisted of newborn calf serum, Eagle's minimum essential medium, glutamate and antibiotics. This method allowed observation of the contractions of the seminiferous tubules in the culture. Contractility, light and electron microscopic structure and histochemically demonstrable activities of alkaline phosphatase and ATPase of the tubule walls were studied at 1-week intervals. The contractility and alkaline phosphatase activity were maintained in the tubule wall for 3 weeks, and the activity of ATPase was maintained for 5 weeks. The thin filaments of the myoid cells, which are responsible for the contractility, were seen with the electron microscope in tubules cultured for 5 weeks. The organ culture method described in the present paper seems to be valuable for studies concerning the functioning of the myoid cells of the seminiferous tubules and the possibility that this is regulated by hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306955
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructural evidence for exocytosis in the median eminence of the rat (1972)
    Springer
    Cell & tissue research 131 (1972), S. 99-107 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Exocytosis ; Micro-pinocytosis ; Releasing factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Exocytosis has been demonstrated by electron microscopy in the external zone of the median eminence of the rat. Exocytotic profiles have been observed in nerve fibres characterized by the presence of granular vesicles with median diameters of 90–103 nm and agranular vesicles of about 50 nm. In addition to the small agranular vesicles, coated vesicles of the same size have been found in many nerve fibres, suggesting that at least part of the agranular vesicles in the median eminence originate by micro-pinocytosis. The nature of the fibres showing exocytosis is discussed. Attention is drawn to the possibility of identifying types of fibres in the median eminence by the occurrence of exocytosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307203
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  • 8
    Electronic Resource
    Electronic Resource
    Form und Verteilung subependymaler Basalmembranlabyrinthe am Ventrikelsystem der Ratte (1972)
    Springer
    Cell & tissue research 132 (1972), S. 553-562 
    Details
    ISSN: 1432-0878
    Keywords: Basement membrane ; Brain ventricles ; Rat ; Subependymal labyrinths ; Light- and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Mit der Perjodsäure-Bisulfit-Aldehydthionin-Färbung (Specht) lassen sich am ganzen Ventrikelsystem des Rattengehirns sub- und interependymale Strukturen lichtmikroskopisch nachweisen, die elektronenmikroskopisch als Basalmembranlabyrinthe (BML) identifiziert werden können. Verschiedene Formen dieser BML lassen sich unterscheiden. Sie stehen in Verbindung mit subependymalen Kapillaren. Die Verteilung der BML wird in Ventrikelkarten dokumentiert.
    Notes: Summary Sub- und interependymal structures, being demonstrable by a period-acid-bisulfit-aldehydthionine-method (Specht), occur in the whole ventricular system by light microscopy. They can be identified electron microscopically as basement membrane labyrinths (BML). Different shapes of these BML can be distinguished. They are in communication with the basement membrane of subependymal capillaries. The distribution of BML is documented in “ventricular maps”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306641
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  • 9
    Electronic Resource
    Electronic Resource
    Ultrastructural studies on the hypothalamic neurosecretory neurons of the rat (1972)
    Springer
    Cell & tissue research 134 (1972), S. 205-225 
    Details
    ISSN: 1432-0878
    Keywords: Diabetes insipidus ; Rat ; Hypothalamo-neurohypophysial system ; Synthesis of hormones ; Light- and Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The general ultrastructural features of the hypothalamo-neurohypophysial system in rats with hereditary hypothalamic diabetes insipidus (DI-rats, Brattleboro strain) are described. There is no decisively distinguishing difference between the neurons of the supraoptic and paraventricular nuclei. The neurons of both nuclei show signs of active protein synthesis. The perikarya of the neurons are markedly hypertrophic, the nuclei are large and the nucleoli prominent. In the cytoplasm there are numerous ribosomes, abundant rough-surfaced endoplasmic reticulum and extensive Golgi complexes. However, very few neurosecretory granules are to be seen. The axons of the hypothalamo-neurohypophysial tract are likewise enlarged and the paucity of neurosecretory granules is a striking feature also in the area of the tract. The majority of nerve endings in the posterior pituitary of DI-rats are devoid of neurosecretory granules. Microvesicles are abundant in the nerve endings and there are findings which suggest that microvesicles are involved either in endoor exocytosis. The signs of active protein synthesis and the concomitant paucity of neurosecretory granules are interpreted to imply transportation of the secretory proteins in an extragranular phase. The possible mode of release of the secretory proteins from the nerve endings and the role of microvesicles therein are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307154
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  • 10
    Electronic Resource
    Electronic Resource
    On the fine structure of the perivascular cells in the neural lobe of rats (1972)
    Springer
    Cell & tissue research 132 (1972), S. 25-34 
    Details
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Rat ; Perivascular cells ; Neurosecretion ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The purpose of this study is to investigate the ultrastructural features of perivascular cells as found in the neural lobe of the hypophysis. In particular, an inquiry was made into the nature of the relationship of such cells to neurosecretory fibers and endings. The latter, in fact, are often invaginated within the perivascular cells and enveloped by their processes; furthermore, they often reveal a certain number of empty granules as well as characteristics of degenerative nature. In the course of this study the localization of the perivascular cells has been investigated as well as that of their processes within the extensive interlobular network typical of the hypophysial neural lobe of rodents. Based on the data gathered, the hypothesis is put forward that the perivascular cells play an important role in the turnover of neurosecretory endings, both under physiological and experimental conditions, contributing thereby to the release of post-hypophysial hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310294
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  • 11
    Electronic Resource
    Electronic Resource
    Elementary granules, small vesicles and exocytosis in the rat neurohypophysis after acute haemorrhage (1972)
    Springer
    Cell & tissue research 125 (1972), S. 277-288 
    Details
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Rat ; Exocytosis ; Freeze-etching ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neural lobes of rats subjected to severe acute haemorrhage under sodium pentobarbitone anaesthesia were examined electron microscopically and the ultrastructure compared with that in anaesthetised and unanaesthetised controls. Changes in the localisation and numerical distribution of elementary granules and small vesicles in the neurohypophysial nerve endings of bled rats were consistent with the occurrence of exocytosis. The occurrence of “exocytotic profiles” was observed more frequently in freeze-etched tissue samples as compared with the material fixed for conventional electron microscopy. The ratio of small vesicles: elementary granules was shown to be significantly increased (P〈0.005) in the nerve endings of neural lobes from bled rats. Equally, the numbers of exocytotic profiles related to 1000 μm2 of neurohypophysial tissue area were significantly greater (P〈0.005) in bled rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306626
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  • 12
    Electronic Resource
    Electronic Resource
    Extracellular perivascular connective tissue space in the medial terminal nucleus of the accessory optic system in rats (1972)
    Springer
    Cell & tissue research 125 (1972), S. 289-294 
    Details
    ISSN: 1432-0878
    Keywords: Accessory optic system ; Rat ; Blood vessels ; Extracellular space ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two types of capillaries were found in the medial terminal nucleus of the rat accessory optic system. Type one capillaries are surrounded by glial processes and lack a perivascular space, whereas the type two capillaries and arterioles show a distinct extracellular perivascular space often filled with collagen fibrils. An internal as well as an external basal lamina lines these spaces. No fenestration of the endothelium was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306627
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  • 13
    Electronic Resource
    Electronic Resource
    Further studies on synaptic junctions (1972)
    Springer
    Cell & tissue research 125 (1972), S. 415-431 
    Details
    ISSN: 1432-0878
    Keywords: Synapses ; Rat ; Cerebral cortex ; Glutaraldehyde/E-PTA ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptic junctions in intact rat cerebral cortex have been examined following glutaraldehyde fixation and phosphotungstic acid (PTA) staining. In the presynaptic ending the network has a hexagonal arrangement, while the dense projections are regularly placed along the presynaptic membrane. Cleft densities occupy the intracleft region. The postsynaptic thickening extends uninterrupted along the length of the junction. Qualitatively, the majority of junctions fall into the ‘discontinuous-continuous’ category, in which the internal coat of the presynaptic membrane together with its associated dense projections is discontinuous along the length of the junction, whereas the postsynaptic thickening is continuous. By contrast, a small number of junctions are ‘continuous-continuous’. In an attempt to analyze the junctions quantitatively, nine indices were measured. Histograms of the size distributions of seven of these appear to be bimodal, and from this it is concluded that two junction populations may be distinguishable on quantitative grounds. It is also shown that the distance separating dense projections at the presynaptic membrane is of the order of 10–15 nm. This surprisingly low value has consequences for current ideas on the relationship between synaptic vesicles and dense projections, and these are discussed at length.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306651
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  • 14
    Electronic Resource
    Electronic Resource
    Die Feinstruktur der interstitiellen Grundsubstanz im inneren Nierenmark der Ratte bei verschiedenen Fixierungsarten (1972)
    Springer
    Cell & tissue research 125 (1972), S. 404-414 
    Details
    ISSN: 1432-0878
    Keywords: Renal medulla ; Rat ; Interstitial ground substance ; Fixation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Interstitium des inneren Nierenmarkes von Ratten wurde nach Anwendung verschiedener Fixierungsarten und Versuchsanordnungen elektronenmikroskopisch untersucht. Neben Bindegewebszellen und kollagenen Fibrillen enthält der interstitielle Raum bandförmige, basalmembranartige Strukturen, in deren weiträumigen Maschen zwei weitere Komponenten, nämlich ein feingranuläres und feinfilamentäres Material sowie kontrastreiche, globuläre Partikel liegen. Diese beiden Formelemente stellen sich bei den verschiedenen Fixierungsarten und Versuchsanordnungen unterschiedlich dar. Bei der feingranulären und feinfilamentären Komponente handelt es sich wahrscheinlich um interstitielles Blutplasma. Diese Deutung wird gestützt durch die morphologische Ähnlichkeit mit dem Blutplasma der Blutgefäße, die unterschiedliche Darstellbarkeit nach Glutardialdehyd-und Formalinfixierung sowie durch die Befunde nach Gefäßperfusion und Nierenvenenstauung. Das Verhalten der globulären Partikel nach OsO4-Einwirkung steht in Einklang mit der schon früher gegebenen Interpretation als präzipitierte saure Mukopolysaccharide. Ihr elektronenoptisches Bild wird im Vergleich zur bekannten makromolekularen Struktur der Mukopolysaccharid-Protein-Moleküle diskutiert.
    Notes: Summary The interstitial ground substance in the inner medulla of the rat kidney is examined electron microscopically after fixation with various fixation agents and under different experimental conditions. The papillary interstitium contains besides interstitial cells and few collagen fibrils basement membrane like material and two other components, fine granular-fibrillar elements and globular particles with heavy contrast. The appearance of the two latter interstitial components varies according to the different fixation agents used and to the experimental conditions. The fine granular and fine fibrillar material surrounds the globular particles numerously after glutardialdehyde-fixation but scarcely after formaldehyde-fixation. It increases after renal vein occlusion, but is diminished or absent, if the kidney is rinsed bloodfree. These results allow to conclude that this material represents interstitial bloodplasma. The behaviour of the globular particles after OsO4-fixation agrees well with the earlier interpretation according to which they represent precipitated acid mucopolysaccharides. Their electron microscopic appearance is discussed in comparison with the known macromolecular structure of the acid mucopolysaccharides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306634
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  • 15
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    Further studies on synaptic junctions (1972)
    Springer
    Cell & tissue research 125 (1972), S. 432-447 
    Details
    ISSN: 1432-0878
    Keywords: Synaptosomes ; Synapses ; Rat ; Cerebral cortex ; Glutaraldehyde/E-PTA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Working with glutaraldehyde fixed, PTA stained rat cerebral cortex, the authors compared the ultrastructural features of synaptosomes with those of intact synaptic junctions. In general there is close correspondence between the two, although the cleft densities and postsynaptic focal densities of synaptosomes show a greater degree of focalization than their counterparts in synaptic junctions. The dense projections have similar profiles in both preparations, but are more difficult to distinguish clearly in synaptosomes on account of the closer packing of the presynaptic network around their apices. The limiting membrane of the presynaptic terminal is usually visible in synaptosomes, but not in synaptic junctions. Comparing the preparations quantitatively reinforces the qualitative findings, and points to their overall similarity. However a number of the indices in synaptosomes are significantly smaller than the corresponding ones in synaptic junctions, and this points to the operation of a shrinkage factor during fractionation procedures. This is confined to the pre- and post-synaptic components and does not affect the intervening contact region. Histograms of the size distributions of the indices are similar to those obtained for intact synaptic junctions, the majority displaying two peaks. It is concluded that synaptosomes accurately reproduce the major ultrastructural features of synaptic junctions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306652
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  • 16
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    On the fine structure of alteration of the adrenal cortex in hypophysectomized rats (1972)
    Springer
    Cell & tissue research 125 (1972), S. 480-496 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Normal ; Hypophysectomy ; Structural alteration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adrenal cortex of normal and hypophysectomized rats was observed by electron microscopy 5–90 days after the operation and, chiefly, the degeneration mechanism of the cortical tissue was discussed. In normal rats mitochondrial inner structures are generally tubular in shape in glomerulosa cells, vesicular (and tubulo-vesicular) in fasciculate cells and tubulo-vesicular (and vesicular) in reticularis cells. Some mitochondria in a few glomerulosa cells show long straight tubular crests in normal as well as hypophysectomized rats. Usually lipid droplets in normal glomerulosa, fasciculate and reticularis cells are surrounded by elements of smooth endoplasmic reticulum, which are very closely associated with mitochondria. About 10–25 days after hypophysectomy, most mitochondria in fasciculate and reticularis cells show tubular crests, and numerous electron lucent lipid droplets are accumulated in these cells. These droplets occupy most parts of the cytoplasm and are sometimes in contact with one another or with mitochondria. The elements of smooth endoplasmic reticulum and Golgi apparatus are markedly reduced in number and size. About 10–90 days after the hypophysectomy, the degenerative cortical cells containing irregularly shaped nuclei, heterogeneously dense bodies in various sizes, and a large number of electron lucent lipid droplets are seen. The author speculates that most fasciculate and reticularis cells degenerate because of a disorder in the lipid metabolism, since ACTH release, which is necessary for synthesis of corticosteroid hormones from cholesterol, is absent in hypophysectomy. The fatty degeneration of the fasciculate and reticularis cells is considered to be a main cause for attenuation of the cortical tissue. Sometimes atrophied cortical cells without lipid droplets are also found 60–90 days after hypophysectomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306655
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  • 17
    Electronic Resource
    Electronic Resource
    The manchette in Stage 14 rat spermatids: A possible structural relationship with the redundant nuclear envelope (1972)
    Springer
    Cell & tissue research 124 (1972), S. 1-11 
    Details
    ISSN: 1432-0878
    Keywords: Spermiogenesis ; Rat ; Spermatids ; Manchette ; Microtubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The manchette or caudal tube has been examined in Stage 14 rat spermatids. The microtubules of the caudal tube have been found to be partially sheathed by smooth endoplasmic reticulum which appears to be continuous with the outer nuclear membrane of the redundant nuclear envelope. The microtubules in caudal regions of the manchette have been noted to be interconnected by links of unusual size and morphology. It is suggested that the caudal tube consists at this stage of development of two structures, membrane and microtubules and that the links between the microtubules appear to play a role in the structural order noted in the position of the tubules of the manchette. The possible significance of these links in relation to motility is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00981937
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  • 18
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    Ultrastructural study of neurovascular contacts in the median eminence of the rat (1972)
    Springer
    Cell & tissue research 124 (1972), S. 311-319 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Neurovascular contacts ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the median eminence of the rat axons of the supraoptic-paraventricular-hypophyseal tract with elementary neurosecretory granules (150–200 mμ) traverse the internal zone. Terminals containing dense core vesicles 60–120 mμ in diameter end on the portal capillaries of the median eminence. A unique organisation of the primary portal capillaries is shown. Endothelial cells have many fenestrae. The pericapillary space has numerous extensions all of which represent a special zone around the endothelial tube. The fine structural organisation and function of the neurovascular contacts in the median eminence are discussed.
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    Pituitaries of rats neonatally treated with oestrogen (1972)
    Springer
    Cell & tissue research 126 (1972), S. 41-52 
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    ISSN: 1432-0878
    Keywords: Pituitary ; Rat ; Oestradiol influence ; STH-cells ; Leucine incorporation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pituitaries of male and female rats neonatally treated with oestradiol dipropionate were examined with light and electron microscopy, disc electrophoresis and in vitro C14-leucine incorporation. Single dose of oestradiol administered shortly after birth to male rats has an prolonged stimulative effect on LTH synthesis and release. This is expressed in the cell hypertrophy, proliferation of ER, increase of corresponding band density and higher rate of 14C-leucine incorporation. In females this effect was less pronounced. STH cell in the treated male pituitaries were degranulated, corresponding hormone band density significantly lowered and incorporation of 14C-leucine was reduced. This could be considered as an inhibitory effect of oestradiol on STH synthesis and release. Such effect was more expressed two than four months after treatment and is more pronounced in male than in female rats.
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    URL: http://dx.doi.org/10.1007/BF00306779
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    Autoradiographische Untersuchungen zum Wachstum der Nebennierenrinde der Ratte (1972)
    Springer
    Cell & tissue research 126 (1972), S. 421-430 
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    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Growth ; Sex-dimorphism ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Anhand des Verlaufs des 3H-Index und der Mitoserate in Anhängigkeit vom Lebensalter wurde die Proliferationsaktivität in den Zonen der Nebennierenrinde untersucht. 84 SPF-Ratten erhielten 2 μCi/g 3H-Thymidin i.p.; der Untersuchungszeitraum erstreckte sich vom 18. Trächtigkeitstag bis zur 12. Lebenswoche. Alle Zonen zeigten in der Rangfolge Glomerulosa — Fasciculata — Reticularis eine Abnahme DNS-synthetisierender Zellkerne. Geschlechtsspezifische Unterschiede im Ausmaß der Proliferationsaktivität konnten zu keiner Zeit nachgewiesen werden. Aus der Dissoziation der Kurven des 3H-Index und der Mitoserate bei vergleichbaren DNS-Syntheseraten wird auf eine Änderung der G2-Phase des Generationszyklus der NNR-Zellen in der teilexponentiellen Wachstumsphase geschlossen.
    Notes: Summary In 84 SPF-rats the poliferative activity of the adrenal cortical cells was studied from the 18th day of pregnancy up to 12 weeks post partum. Rats were given 2 μC/g tritiated thymidine and killed 1 hour thereafter. It was shown that there was no sex-related difference in the degree of proliferation that could explain the sexual dimorphism in adrenal weights. In all cortical zones a decrease in the number of labeled cells was seen during the obvservation period. The highest percentage of labeled cells was found in the glomerulosa. There exists no parallelism of the mitotic and labeling index, which gives evidence for a change in length of the G2-phase of the cell generation cycle.
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    Ultrastructural features of the lateral preoptic area, median eminence and arcuate nucleus of the rat (1972)
    Springer
    Cell & tissue research 127 (1972), S. 483-491 
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    ISSN: 1432-0878
    Keywords: Lateral preoptic area ; Hypothalamus ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The arcuate nucleus, median eminence, and the lateral preoptic area from the brains of aldehyde-perfused male and female rats were examined by electron microscopy. In the lateral preoptic area, three neuronal types are described: a small light neuron, a larger light one, and a dark neuron resembling the larger light one in size and nuclear shape. Many myelinated axons are interposed among single neurons or neuronal pairs. The relationship of structures to each other is discussed. Several observations not previously reported are illustrated from tissue of the arcuate nucleus and median eminence.
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    Distribution of monoamine oxidase in the hippocampal region of the rat (1972)
    Springer
    Cell & tissue research 124 (1972), S. 354-366 
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    ISSN: 1432-0878
    Keywords: Hippocampal region ; Rat ; Monoamine oxidase ; Chemoarchitectonics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. The distribution of monoamine oxidase (MAO) in the hippocampal region of the rat has been studied in cryostat sections with the tryptamine-tetrazolium method of Glenner et al. 2. The staining pattern was rather diffuse as compared with the distribution of MAO in the guinea pig and of other enzymes in the rat hippocampal region. 3. However, there were differences in enzyme activity between different areas and layers which could be correlated to some degree with results obtained with other methods, e.g., silver impregnation, catecholamines, and acetylcholinesterase.
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    Monoamines in rat thyroid parafollicular cells and the effect of vitamin D2-induced degranulation (1972)
    Springer
    Cell & tissue research 128 (1972), S. 406-425 
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    ISSN: 1432-0878
    Keywords: Parafollicular Cells ; Rat ; Normalcalcemia ; Vitamin D2 ; Electronmicroscopy ; Histochemical fluorescence method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Thyroid parafollicular cells of normocalcemic and vitamin D2-treated rats were investigated by electron microscopy and with the histochemical fluorescence technique of Hillarp and Falck. Administration of high doses of vitamin D2 caused hypercalcemia and an extensive degranulation of the parafollicular cells. The formation and storage of monoamines in granulated and degranulated parafollicular cells was investigated by fluorescence microscopy after injection of monoamine precursors (DOPA, 5-HTP), alone or in combination with Ro 4-4602, nialamide or reserpine. No fluorescence was observed in parafollicular cells of untreated rats. l-DOPA and l-5-HTP (but not the corresponding D-amino acids) were taken up by a process closely linked to the decarboxylation of the amino acids to the corresponding amines (dopamine and 5-hydroxytryptamine). Treatment with vitamin D2 did not seem to affect the formation of amines in the parafollicular cells or the formation and storage of amines in other cell systems investigated. The amine itself (dopamine) was not taken up by the parafollicular cells. In normocalcemic rats, the amine formed was retained in the cytoplasm of the parafollicular cells by a partially reserpine-resistant mechanism. The storage of amines is concluded to occur in association with the calcitonin-containing granules. In parafollicular cells of vitamin D2-treated rats, a certain amount of amine was bound in the cytoplasm in the absence of typical granules. As a considerable amount of calcitonin is known to remain in the thyroid of vitamin D2-treated rats, the present observations may indicate an association between the amine and the polypeptide hormone calcitonin, whether the latter is confined to typical granules or not.
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    Electron microscope study of the myofibril partial disintegration and recovery in the mitotically dividing cardiac muscle cells (1972)
    Springer
    Cell & tissue research 129 (1972), S. 471-499 
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    ISSN: 1432-0878
    Keywords: Cardiac muscle cells ; Rat ; Mitotic division ; Myofibril changes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the myocyte at all phases of mitosis as well as of early postmitotic cells has been studied in the myocardia of 14- and 18-day rat embryos and 5- and 7-day old rats. The myofibrils remain unchanged up to the late prophase. In prometaphase the majority of Z-disks in embryo myocyte myofibrils and considerable part of these disks in myofibrils of suckling rats are drastically disintegrated. This is followed by a progressive isolation and scattering of the myofilament bundles and of the whole sarcomeres during the subsequent phases of mitosis. Thick myofilaments seem to be unchanged but thin ones become frequently poorly outlined (mainly in embryos). The sarcoplasmic reticulum, including its typically differentiated subsarcolemmal cisternae, exhibits relatively few changes during mitosis. In the early postmitotic period there is a gradual restoration of contrast-rich Z-bands, interconnecting the previously isolated sarcomeres. Patterns of this process have much in common with early stages of myofibrillogenesis (appearance of subsarcolemmal “Z-bodies”, formation of skeins of thin filaments etc.). The cleavage furrow formation is either absent or considerably retarded up to the postmitotic period. Behaviour of some other organelles during myocyte mitosis has been described. Possible mechanisms and significance of the observed phenomena are discussed.
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    Zur Ultrastruktur der Gefäßfortsätze von Ependymund Gliazellen im Infundibulum der Ratte (1972)
    Springer
    Cell & tissue research 130 (1972), S. 58-69 
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    ISSN: 1432-0878
    Keywords: Infundibulum ; Rat ; Ependymal and glial cells ; Inclusions ; Neuroglial synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Zahlreiche Ependymzellen des Recessus infundibularis und Gliazellen der Zona externa besitzen Fortsätze, die bis an den perivaskulären Raum der Kapillarschlingen des Portalplexus heranreichen. Die Ultrastruktur dieser Gefäßfortsätze wird beschrieben. Sie können verschiedenartige Einschlüsse enthalten: 1. große, runde osmiophile Einschlüsse (0,5–2 μ im Durchmesser), die als „lipid inclusions“ bezeichnet werden; 2. unregelmäßig geformte, granuläre Einschlüsse mit gleichmäßiger Elektronendichte (500 bis 2000 Å im Durchmesser); 3. rundliche Granula mit einem hellen Zentrum und ringartig um dieses Zentrum gelagerten elektronendichten Körnchen (1200–1600 Å im Durchmesser). Der letztgenannte Granulatyp scheint ein charakteristisches Merkmal der Gefäßfortsätze in der Zona externa der Wistar-Ratte zu sein. Meist häufen sich die Körnchen gemeinsam mit den anderen beschriebenen Einschlüssen in den kolbenförmigen Endigungen der Gefäßfortsätze. Exocytosevorgänge an der dem perivaskulären Raum zugewandten Oberflächenmembran der Fortsätze weisen auf eine Abgabe von Substanzen an die Blutbahn hin. Neurogliöse Synapsen finden sich im Infundibulum vorwiegend zwischen Nervenfasern des Tractus tuberohypophyseus und Gefäßfortsätzen der Ependym- und Gliazellen. Der synaptische Spalt enthält häufig fädige oder körnige Strukturen. Solche Partikel finden sich zusammen mit tubulären oder vesikulären Membranprofilen auch im synapsennahen Bereich des Glia- oder Ependymfortsatzes. Die funktionelle Bedeutung dieser Synapsen insbesondere für die Abgabe neuronaler Substanzen wird diskutiert.
    Notes: Summary Many ependymal cells of the infundibular recess and many glial cells of the external layer send their processes to the perivascular space of capillary loops of the portal plexus. The ultrastructure of these vascular processes is described. They may contain various inclusions: (1) large, osmiophilic globules (0,5–2 μ in diameter), mostly termed “lipid inclusions”; (2) irregularly formed, granular inclusions with an evenly distributed content (500–2000 Å in diameter); (3) circular granules comprising an electron-lucent centre surrounded by an annular wall of electron-dense particles (1200–1600 Å in diameter). This type of granule seems to be characteristic for the vascular processes in the external layer of the Wistar rat. Frequently accumulations of these granules are found together with other inclusions in the widened end-feet of the vascular processes. The occurrence of exocytosis at the perivascular surface membrane of the vascular processes indicates a release of substances into the blood. Neuro-glial synapses in the infundibulum are mainly found between nerve fibres of the tuberohypophyseal tract and the vascular end-feet of ependymal and glial cells. The synaptic cleft often contains filamentous or granular material. Together with tubular and vesicular membrane profiles such particles are also found in the postsynaptic area of the ependymal and glial processes. The functional significance of these contacts is discussed particularly with respect to the release of neuronal substances.
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    Isolation and characterization of neurosecretory granules of the rat posterior pituitary gland (1972)
    Springer
    Cell & tissue research 129 (1972), S. 92-113 
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    ISSN: 1432-0878
    Keywords: Neurosecretion ; Neurohypophysis ; Neurosecretory granule ; Rat ; Membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neurosecretory granules (NSG) of rat posterior pituitary glands were prepared by differential centrifugation techniques mainly according to the procedure as described by Barer, Heller and Lederis (1963). As revealed by electron microscopy, the recovery of neurophysin and the contents of enzymes, purified NSG were obtained in a pellet at 30 000 g/60 min (0.44 M sucrose). Eighteen h after injection of (35S) cysteine into the supraoptic nucleus 60% of the recovered radioactivity in the neural lobe was found in the NSG, whereas 20% was found in the final supernatant (100 000 g/120 min). Sixteen days after injection the NSG and the final supernatant fraction contained fairly equal amount of (35S) cysteine (approximately 40%). It is suggested that after a period of intragranular maturation neurophysin is extruded into an extragranular pool of neurosecretory material. With the use of conventional polyacrylamide-gel electrophoresis it was shown that the predominating proportion of radioactivity in the NSG after a hypothalamic injection of (35S) cysteine was located within the neurophysin fraction A and in fraction B. Fraction B is suggested to be partly bound to the NSG membranes. When the NSG soluble and NSG insoluble proteins, obtained after lysis of NSG, were separated on polyacrylamide gels in the presence of sodium dodecylsulphate, the highly radioactive soluble protein was shown to consist of two components with average molecular weights of 12 300 and 14 600. Most of the proteins in the lysate were found in the NSG membranes, though less radioactive. A component with a mol.wt. of 37 000 was enriched in the membrane fraction. At longer times after isotope injection the high mol.wt. proteins, particularly those of the NSG membranes, contained increased amounts of radioactivity.
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    Electron microscopy of filaments in the basal part of rat kidney tubule cells, and their in situ interaction with heavy meromyosin (1972)
    Springer
    Cell & tissue research 132 (1972), S. 497-521 
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    ISSN: 1432-0878
    Keywords: Kidney ; Rat ; Actin- and myosin-like filaments ; Tubule and interstitial cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By electron microscopy, the prominent bundles of filaments occurring in the basal part of proximal and distal tubule cells and in interstitial cells of rat kidney cortex were studied in cells fixed by vascular perfusion, in glycerol-extracted cells and in glycerol-extracted cells treated with heavy meromyosin (HMM). The studies of perfusion-fixed tissue showed that the proximal tubule cells contained in their most basal part filamentous bundles oriented transversely around the tubule. The bundles consisted of trightly packed thin filaments (50–80 Å in diameter). Similar but less prominent bundles were found in distal tubule cells and in interstitial cells. The dimension of these filaments was similar to that of actin filaments and their insertion in the basal cell membrane of the tubule epithelial cells resembled the insertion of actin filaments in the cell membrane of smooth muscle cells. The studies on glycerol-extracted cells revealed that some tubule cells contained two types of filaments (60–80 Å and 130–170 Å in diameter) located side by side in the basal filamentous bundles. The dimension of the thick filaments corresponds well to the values for myosin filaments in glycerinated smooth and skeletal muscle. The studies on HMM-reacted renal tissue revealed that the thin filaments (60–80 Å) described in tubule and interstitial cells are probably actin filaments, as they formed characteristic arrowhead complexes morphologically indistinguishable from the complexes of HMM with actin filaments in smooth and striated muscle cells. Our results provide strong evidence that a two-filament contractile system, based on interaction of actin and myosin filaments, exists in renal tubule and interstitial cells. As a hypothesis it is proposed that it is changes in tonus of the basal filamentous system in the proximal tubule cells which stabilize the intratubular pressure, possibly via angiotensin.
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    The manchette in Stage 14 rat spermatids: A possible structural relationship with the redundant nuclear envelope (1972)
    Springer
    Cell & tissue research 124 (1972), S. 1-11 
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    ISSN: 1432-0878
    Keywords: Spermiogenesis ; Rat ; Spermatids ; Manchette ; Microtubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The manchette or caudal tube has been examined in Stage 14 rat spermatids. The microtubules of the caudal tube have been found to be partially sheathed by smooth endoplasmic reticulum which appears to be continuous with the outer nuclear membrane of the redundant nuclear envelope. The microtubules in caudal regions of the manchette have been noted to be interconnected by links of unusual size and morphology. It is suggested that the caudal tube consists at this stage of development of two structures, membrane and microtubules and that the links between the microtubules appear to play a role in the structural order noted in the position of the tubules of the manchette. The possible significance of these links in relation to motility is discussed.
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    Ribosomes in myelinated axons of dorsal root ganglia (1972)
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    Cell & tissue research 124 (1972), S. 217-229 
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    ISSN: 1432-0878
    Keywords: Sensory axons ; Dorsal root ganglia ; Rat ; Ribosomes ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the dorsal root ganglia of the rat, ribosomes were found not only in the initial segment, but they were also observed in the axoplasm of intraganglionar myelinated fibres and in the sensory portion of spinal nerves. Axons of seven-days-old rats contained more ribosomes than those of adult animals. The amount of particles decreased gradually from the initial segment trough intraganglionar internodes to the axons of spinal nerves. No ribosomes were found in axons of dorsal roots. In intraganglionar fibres, ribosomal particles were usually observed near the nodes of Ranvier, in the vicinity of Schmidt-Lantermann clefts and in axons near the Schwann cell nuclei. They were arranged in tetrads, pentads or in larger polysomes, and they were often observed adjacent to a group of mitochondria. The particles had invariably a stable size, their average diameters measuring 234 ± 2 × 197 ± 3 Å, which is practically equal to the diameters of 232 ± 2 × 203 ± 3 Å of ribosomes in the Schwann cell cytoplasm. These values fall within the range of diameters of ribosomes isolated from various cells of eukaryotic organisms as given in the literature. Since no other granular component of the cytoplasm has similarly stable dimensions, the measurements are considered to prove that the axonal particles described here are ribosomes.
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    Cellules riches en glycogène dans l'épendyme du ventricule latéral du cerveau de rat (1972)
    Springer
    Cell & tissue research 123 (1972), S. 356-368 
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    ISSN: 1432-0878
    Keywords: Ependyma ; Brain ; Rat ; Glycogen-rich cells ; Electronmicroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ependyma of the lateral ventricle of the rat brain was investigated at different ages from 20 days to adulthood. A particular cell type occurs in the external wall of the ventricle, where the proliferative subependymal layer is present. These cells found at all ages studied, are characterized by a high content of glycogen, and a structure different of typical ependymal cells. A large number of nerve endings is situated in close vicinity of these cells, either free in the ventricle lumen, or sometimes ensheathed in the cells. No synapse was found between these endings an the glycogen-rich cells. These glycogen-rich cells undergo several modifications with age: their glycogen content is reduced in the adult, and they acquire a few cilia and gliofilaments. It is suggested that they represent a transitory differentiation of the ependyma, functionally linked with the proliferative subependymal layer.
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    Peripheral sympathetic innervation and serotonin cells in the habenular region of the rat brain (1972)
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    Cell & tissue research 127 (1972), S. 570-579 
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    ISSN: 1432-0878
    Keywords: Sympathetic nerve fibres ; Rat ; 5-HT-cells ; Habenular region (Lamina intercalaris ; Medial habenular nucleus)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pineal gland of the rat is located near the brain surface and is via a slender stalk connected to lamina intercalaris which constitutes a cell formation between the habenular and posterior commissures, continuing to the subcommissural organ. The stalk and lamina intercalaris, like the pineal proper, exhibited a yellow, formaldehyde-induced fluorescence which showed the histochemical and pharmacological properties of 5-HT. All these structures were richly supplied with catecholamine-fluorescent nerves which could be further followed rostrally from lamina intercalaris, mixing with the non-fluorescent commissural fibres and stria terminalis, into the medial habenular nucleus in which they extensively supplied both blood vessels and non-fluorescent nerve cells. Cytospectrofluorometric and chemical analysis suggested that the fluorescent nerves stored noradrenaline. This was supported by the finding that they disappeared after bilateral cervical sympathectomy (as did the fluorescent nerves in the pineal complex). In the medial habenular nucleus also catecholamine-containing and 5-HT-containing nerves of central origin were present. The occurrence of a rich, peripheral sympathetic innervation in the medial habenular nucleus of the brain offers possibilities for a previously not observed sympathetic influence on this nucleus. Also the arrangement, and the apparent continuity of the sympathetic innervation in the pineal gland, the lamina intercalaris, and the medial habenular nucleus, suggests some functional interconnection or coordination between these structures.
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    Release in vitro of neurohypophysial proteins from neural lobe tissue slices and from isolated neurosecretory granules of the rat (1972)
    Springer
    Cell & tissue research 129 (1972), S. 114-139 
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    ISSN: 1432-0878
    Keywords: Neurosecretion ; Pituitary gland ; Rat ; Neurophysin ; Vasopressin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The release of neurophysin from neural lobe tissue slices and isolated neurosecretory granules of the rat was studied at various time intervals after injection of (35S) cysteine into the supraoptic nucleus. For hours after isotope injection the release of radioactive neurophysin from neural lobe tissue was increased by depolarizing concentration of potassium in the presence of calcium ions. Fourteen and 30 days after isotope injection the release of radioactive neurophysin was relatively decreased in a medium of high potassium concentration which might be explained by the heterogeneity of the pool of neurophysin within the neural lobe. Four h after isotope injection the “spontaneous” release of neurophysin from neural lobe tissue was higher in dehydrated rats than in controls, and the neural lobes of these animals did not respond with an increased release of radioactive neurophysin when exposed to high potassium concentration. Eighteen h after isotope injection the predominating proportion of neurophysin-bound radioactivity was found in the neurosecretory granule fraction, whereas 14 days after injection a fairly equal amount of radioactivity was found in this fraction and in the “soluble” protein fraction. This indicates that with time an increasing amount of radioactive neurophysin passes from an intragranular to an extragranular pool. The spontaneous release of radioactive neurophysin from isolated neurosecretory granules was higher, and the increase of release upon exposure to an ATP-regenerating system was higher 14 days after isotope injection than 18 h after injection. This may imply that the neurosecretory material undergoes an intragranular “maturation” process to become more easily releasable. The release of radioactive neurophysin was inhibited in the presence of AMP and EDTA, which demonstrates the dependence of the release process of an ATPase and, probably, of calcium ions. Neurosecretory granules from dehydrated rats possessed a higher spontaneous release than those of control rats 18 h after injection, which may indicate an enhanced intragranular “maturation” process of the neurosecretory material due to osmotic stimulation.
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    Zur Größe der Fettpartikel in mitochondrienreichen Skelettmuskelfasern der Ratte in Abhängigkeit von der Nahrungsaufnahme (1972)
    Springer
    Cell & tissue research 127 (1972), S. 374-381 
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    ISSN: 1432-0878
    Keywords: Skeletal muscle ; Rat ; Fat droplets ; Fat resorption ; Quantitative electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Elektronenmikroskopisch nachweisbare Neutralfettpartikel kommen im M. tibialis anterior der Ratte vorwiegend in den mitochondrienreichen Fasern vom Typ C in der Nachbarschaft von Mitochondrien vor. Nach Glutaraldehyd-Osmium-Fixierung und Epon-Einbettung sind diese Partikel meist dielektronisch, seltener teilweise oder ganz adielektronisch. Auf 100 μm2 Schnittfläche durch C-Fasern findet man 3–4 Anschnitte von Fettpartikeln. Der mittlere Durchmesser der Anschnitte (d) ist 0,69 μm, der mit der graphischen Methode von Hennig bestimmte mittlere Durchmesser der Fettpartikel (d′) 0,80 μm. 2 h Narkose mit Pentobarbital beeinflussen weder Anzahl noch Durchmesser der Anschnitte. Nach 2 h Resorption von Maiskeimöl ist die Zahl der Anschnitte unverändert, der mittlere Durchmesser der Anschnitte bzw. der Partikel hat um etwa 20 % zugenommen (d=0,83 μm; d′=0,95 μm). Das entspricht einer Zunahme des morphologisch nachweisbaren Neutralfettes um 70 %. Da keine Pinocytose von Chylomikronen zu beobachten ist, wird angenommen, daß freie Fettsäuren durch das Plasmalemm in die Muskelfaser eindiffundieren und im Bereich vorhandener Fettpartikel als Triglyceride gespeichert werden.
    Notes: Summary In the anterior tibial muscle of rats triglyceride droplets predominantly appear within fibres rich in mitochondria (type C). In electron micrographs 3–4 of such droplets are present per 100 μm2 sectional area of C-fibres. The mean diameter of sections through fat droplets (d) is 0.69 μm, the mean diameter of the droplets (d′) as determined by Hennig's graphical method is 0.80 μm. Anaesthesia with pentobarbital (2 h) does affect neither number nor size of the particles. 2 h after application of corn oil into the stomach the number of fat droplets is unaltered, but the mean diameter has increased by 20 % (d=0.83 μm; d′=0.95 μm), corresponding to an increase of the volume of about 70 %. Pinocytosis of chylomicrones by muscle fibres has never been observed. Therefore it is supposed, that free fatty acids have passed the plasmalemma by diffusion and are incorporated into triglycerides and stored within pre-existing fat droplets.
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    An ultrastructural study of the neurosecretory cells of the medial vascular prechiasmatic gland (1972)
    Springer
    Cell & tissue research 127 (1972), S. 439-461 
    Details
    ISSN: 1432-0878
    Keywords: Prechiasmatic gland (Organon vasculosum laminae terminalis) ; Rat ; Nerve endings ; Dense-cored vesicles ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary On the basis of the present electron microscopic study of the rat median vascular prechiasmatic gland, the unmyelinated nerve fibers containing dense-cored vesicles may be classified in three categories. (1) Nerve endings containing isomorphic rounded dense-cored vesicles, 800–1600 Å in diameter with a predominance of vesicles between 1100 and 1300 Å. These vesicles are synthesized in the nerve cell perikarya which are localized principally in the lamina profunda of the outer subpial layer and also in the neuropil of the inner glial covering layer. One or more “cholinergic” axon terminals are in contact with the surface of these nerve cell perikarya. The dense-cored vesicles travel in the axonal process which branches into an extensive terminal network characterized by the presence of small varicosities. They abut on the pericapillary spaces but never against another neuron or effector cell. A few nerve processes course between the ependymal cells and terminate in the preoptic recess of the third ventricle. The dense-cored vesicles may contain one of the primary monoamines. (2) Nerve endings containing pleomorphic dense-cored vesicles, 500–1600 Å in diameter with a predominance of vesicles between 800 and 1100 Å. The latter granular vesicles are associated with small, clear-centered vesicles, 260–550 Å in diameter, which are often aggregated in the vicinity of thickenings of the plasma membrane and of cytoplasmic dense projections. The intercellular space and the cell plasma membrane, however, do not display any modification facing these structures. Synapse-like contacts are observed between these nerve endings and cells containing a great amount of microfilaments and glycogen particles in their cytoplasm. The latter cells possibly represent processes of ependymal cells. (3) Nerve endings containing a few large dense-cored vesicles in addition to a majority of synaptic vesicles.
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    Adrenocorticotropin production by the intermediate lobe of the rat pituitary (1972)
    Springer
    Cell & tissue research 132 (1972), S. 1-14 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary ; Rat ; Pars intermedia ; Adrenocorticotropin ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adrenocorticotropin (ACTH) was localized in the intermediate lobes of normal rat pituitaries with unlabeled antibody and the soluble peroxidase-antiperoxidase complex at the electron microscopic level. All of the intermediate lobe cells (light and dark cells) stained positively for ACTH. Stain of varying intensity was found on secretory granules and vesicles. Structures which resembled rough endoplasmic reticulum stained where the secretory granules and vesicles were most numerous. No stain was on mitochondria, in nuclei, in cisternae and granules of Golgi complexes, or in rough endoplasmic reticulum where there were few secretory granules. There were distinct immunohistochemical differences between the pars intermedia and anterior lobe ACTH cells. In the ACTH cells of the anterior lobe, the Golgi complex and its granules stained strongly, but not at all in the pars intermedia cells. This suggests that ACTH may not be packaged or condensed in the Golgi region of the latter.
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    Sub- und interependymale Basalmembranlabyrinthe am Zentralkanal der weißen Ratte (1972)
    Springer
    Cell & tissue research 132 (1972), S. 217-229 
    Details
    ISSN: 1432-0878
    Keywords: Basement membrane labyrinths ; Spinal cord ; Central canal ; Rat ; Light- and Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Wand des Zentralkanals im Rückenmark der weißen Ratte enthält zahlreiche Basalmembranlabyrinthe; ihre Zahl nimmt in caudaler Richtung deutlich zu. Die Perjodsäure-Bisulfit-Aldehydthionin-Färbung (Specht) gestattet ihren lichtmikroskopischen Nachweis. Die Labyrinthe stehen in Verbindung mit den Basalmembranen der kanalnahen Kapillaren. Sie können auch elektronenmikroskopisch identifiziert werden; ihre Struktur gleicht der der Basalmembranen der Gefäße. Die funktionelle Bedeutung der Basalmembranlabyrinthe wird diskutiert.
    Notes: Summary The wall of the central canal of the spinal cord of the white rat contains numerous basement membrane labyrinths, their number increasing towards the end of the canal. The period-acid-bisulfit-aldehydethionine-method (Specht) permits their light microscopic finding. They are in connection with the basement membranes of the capillaries situated near the canal. The basement membrane labyrinths can be identified by electron microscopy; their structure is similar to that of basement membranes of the capillaries. The functional significance of these structures is discussed.
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    Surface multivesicular structures associated with maturing erythrocytes in rats (1972)
    Springer
    Cell & tissue research 133 (1972), S. 181-186 
    Details
    ISSN: 1432-0878
    Keywords: Surface multivesicular structures ; Erythrocyte maturation ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Surface multivesicular structures associated with the plasmalemma of erythrocytes were observed in the peripheral blood of rats which have a significant number of circulating reticulocytes. These surface structures appear as ovoid evaginations (0.2 to 0.7 μ in diameter) of the plasma membrane and contain numerous small vesicles ranging from 0.05 to 0.1 μ in diameter. The structures were present during the final stages of maturation of erythrocytes, after nuclei and mitochondria had been extruded and only a few polysomes and small vesicles remained. They appear quite distinct from the autophagic vacuoles which have been described in association with degeneration and extrusion of mitochondria from erythrocytes. The exact origin of the small internal vesicles of these surface multivesicular structures is unknown; however, similar vesicles have been observed in the cytoplasm of the maturing erythrocyte especially in the vicinity of the Golgi body. These structures suggest a process by which Golgi elements and other small cytoplasmic vesicles are extruded during the late stages of maturation of rat erythrocytes.
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    URL: http://dx.doi.org/10.1007/BF00307140
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    Zur Morphologie und Funktion der Calcitoninzellen (C-Zellen) der Ratte (1972)
    Springer
    Cell & tissue research 134 (1972), S. 13-30 
    Details
    ISSN: 1432-0878
    Keywords: Rat ; Thyroid C-cells ; Ultrastructure ; Tocopherol and Ubiquinone deficiency ; Substitution (d,1-α-Tocopherol, α-Tocopherolquinone)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung An der Schilddrüse von Wistar-Ratten wurde der Einfluß der Kost (Laborstandardkost, halbsynthetische-tocopherolhaltige und halbsynthetische-tocopherol-freie Kost) und der subkutanen Injektion von Tocopherol und Tocopherolchinon auf die Ultrastruktur der C-Zellen untersucht. Während nach Standardfütterung die Aktivität der C-Zellen der Norm entspricht, werden Syntheseleistung und Sekretabgabe dieser Zellelemente durch die halbsynthetische-tocopherolhaltige Kost gesteigert und im alimentären Tocopherol-Ubichinonmangel wieder gedämpft. Der elektronenmikroskopisch erkennbare Gehalt der C-Zellen an Sekretgranula entspricht der in vitro ermittelten Calcitoninaktivität. Die Beziehungen zwischen den Funktionskreisen von Thyreozyten und Calcitoninzellen werden diskutiert.
    Notes: Summary The influence of different diets (standard laboratory diet, semisynthetic tocopherol-containing and semisynthetic tocopherol-free diet) and the influence of subcutaneous injections of tocopherol and tocopherolquinone upon the ultrastructure of the C-cells in the thyroid gland of Wistar rats were investigated. While the activity of the C-cells is quite normal after feeding a standard diet, the synthetic and secretory activities of these cells show an increase with a semisynthetic diet containing tocopherol, but a decrease during alimentary lack of tocopherol and tocopherolquinone. The amount of secretory granules in the C-cells as seen with the electron microscope corresponds with the in vitro-activity of extracted calcitonin. The relations between the functional system of the thyrocytes and that of the C-cells are discussed.
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    Effect of foreign innervation on the androgen-sensitive levator ani muscle of the rat (1972)
    Springer
    Cell & tissue research 135 (1972), S. 165-174 
    Details
    ISSN: 1432-0878
    Keywords: Musculus levator ani ; Rat ; Androgen sensitivity ; Foreign innervation ; Histochemical Muscle fibre pattern
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cross-union of the tibial with the pudendal nerve innervating the androgen-sensitive levator ani (LA) muscle of male rats, results in reversal of the histochemical muscle fibre pattern concerning myofibrillar ATPase, succinate dehydrogenase and phosphorylase enzyme activities. The homogeneous muscle fibre pattern of the LA muscle is changed to a mosaic pattern of muscles normally innervated by the tibial nerve. The success of the hetero-reinnervation is shown by practically full recovery of muscle weight and of isometric twitch-contraction properties of the LA muscle. Castration of 2-months duration, i. e. lack of the male sex hormone, leads to marked atrophy but no change in histochemical muscle fibre pattern. Hetero-reinnervation of the LA muscle results in change of histochemical enzyme pattern even if the cross-union of nerves is performed after long periods of castration leading to very marked decrease of muscle fibre size. However, testosterone application alone after castration increases markedly muscle fibre size but does not lead to reversal of muscle fibre pattern. The myotropic hormonal influence on the target (LA) muscle is therefore primarily of myogenic origin and specificity of hormonal action is maintained even with a foreign nerve innervating the muscle. The experiments, thus, provide evidence for the differentiation of specific neural influences affecting muscle fibre pattern and hormonal influences in respect to the myotropic action of the sex hormone on the androgen-sensitive LA muscle.
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    Autoradiographische Bestimmung der S-Phasen-Dauer der Gonocyten bei der Wistarratte durch Einfach- und Doppelmarkierung (1972)
    Springer
    Cell & tissue research 125 (1972), S. 229-251 
    Details
    ISSN: 1432-0878
    Keywords: Gonocytes ; Rat ; Mitotic activity ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Gonocyten der Ratte können in 2 hintereinander geschaltete Keimzellarten gegliedert werden, die I-Gonocyten und II-Gonocyten. Die I-Gonocyten proliferieren bei der Wistarratte zwischen 15. und 18. Fetaltag, die Tochterzellen der I-Gonocyten, die II-Gonocyten treten nach einer Zeitdauer von 7–8 Tagen zwischen 4. und 6. Lebenstag in die Mitose. Auf Grund der gewonnenen Daten erschien es sinnvoll, die mitotische Aktivität der II-Gonocyten und die Bestimmung der Dauer ihrer S-Phase an 5 Tage alten Ratten durchzuführen. Untersuchungen von 50 Zentren mitotischer Aktivität in einem in Serie geschnittenen Hoden einer 5 Tage alten Ratte ergaben, daß 148 von 190 Mitosen, d.s. 78%, in Gruppen und 122, d.s. 64% der Mitosen in „Paaren“ vorkommen. Mit der Methode der markierten Mitosen (Quastler u. Sherman, 1959) und der Methode der Doppelmarkierung (Hilscher u. Maurer, 1962) wurde die Dauer der S-Phase der II-Gonocyten bei 5 Tage alten Ratten bestimmt. Es ergab sich eine gute Übereinstimmung der nach beiden Methoden bestimmten Werte. Die S-Phasen-Dauer der II-Gonocyten dürfte danach am 5. Lebenstag bei 11,0–11,5 Std liegen.
    Notes: Summary The gonocytes of the rat are of two types: I-gonocytes and II-gonocytes. In Wistar rat I-gonocytes proliferate at the beginning of prespermatogenesis between the 15th and 18th day of gestation. Their multiplication stops between the 18th and 19th day. Starting on the 4th postnatal day, II-gonocytes, the daughter cells of I-gonocytes, begin to proliferate. The 5th postnatal day proved to be favourable for studying the mitotic activity and for determing the S-phase of II-gonocytes. In one serially sectioned testis of a 5 days old rat 25 sex cords were reconstructed. Till now 50 centres of mitotic activity of II-gonocytes with 190 mitoses were localized. Only 42 out of the 190 mitoses were isolated, 148 occur in groups. 122 out of the grouped mitoses are in “pairs”. That means that 78% of the grouped and 64% of all mitoses were to be found in pairs. By the method of labeled mitoses (Quastler and Sherman, 1959) and by the method of double labeling with C-14- and H-3-thymidine (Hilscher and Maurer, 1962) the duration of the S-phase of II-gonocytes were determined in 5 days old rats. The results of both methods show that the S-phase is 11.0 to 11.5 hours.
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    Über die Reaktion von Phenylradikalen mit Methanol (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 33-33 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Tab.
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    URL: http://dx.doi.org/10.1002/ange.19720840112
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    Über die Abhängigkeit der Reaktionsfähigkeit der Doppelbindung in höheren Olefinen von der Stellung und der geometrischen Konfiguration (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 37-37 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ange.19720840117
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    Solvolyseversuche mit 7,7-Bis(alkylthio)norcaranen. Eine neue Methods der Homologisierung (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 60-61 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ange.19720840205
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    Amino-pentalencarbonitrile, eine Gruppe stabiler Pentalene (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 61-62 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ange.19720840206
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    7-Isopropylidennorbornadien (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 65-67 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/ange.19720840210
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    Autoradiographie. Von H. A. Fischer und G. Werner. Walter de Gruyter & Co., Berlin 1971. 1. Aufl., IX, 214 S., 93 Abb., 14 Tab., geb. DM 42.- (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 74-74 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
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    Dehydro-N-Mannich-BasenDiese Arbeit wurde von der Deutschen Forschungsgemeinschaft und dem Fonds der Chemischen Industrie unterstützt. (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 109-109 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ange.19720840307
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    Synthese und Impuls-Fourier-Transform-13C-NMR-Untersuchungen verzweigter KohlenhydrateDiese Arbeit wurde von der Ligue Nationale Francaise contre le Cancer unterstützt. (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 111-112 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Sesquifulvalene aus DihydrofulvalenenDiese Arbeit wurde von der Deutschen Forschungsgemeinschaft und dem Fonds der Chemischen Industrie unterstützt. (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 115-117 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Dyotrope Umlagerungen, eine neue Klasse orbitalsymmetrie-gesteuerter Reaktionen. Typ I (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 161-162 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Leitfaden der Farbstoffchemie. Von P. Rys und H. Zollinger. Chemische Taschenbücher Band 13. Herausgeg. von W. Foerst und H. Grünewald. Verlag Chemie GmbH, Weinheim/Bergstr. 1970. 1. Aufl., XI, 220 S., 16 Abb., 16 Tab, geb. DM 22.- (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 176-176 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Überbrückte Hetero[11]annulene (1972)
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    Zeitschrift für die chemische Industrie 84 (1972), S. 207-208 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Übergangsmetall-Carben-Komplexe als π-Liganden - [Äthoxy(2,6-dimethoxyphenyl)carben-tetracarbonyleisen(0)]tricarbonyleisen(0) (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 214-214 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Struktur des 2:1-Cycloadditionsproduktes von Diazomethan und 1,1,1-Trifluor-2-trifluormethyl-3-aza-2,4-trans-hexadien (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 212-214 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    URL: http://dx.doi.org/10.1002/ange.19720840509
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    Photooxygenierung von 3,4-Diäthyl-2,5-dimethylpyrrolDiese Arbeit wurde von der National Science Foundation und dem Petroleum Research Fund der American Chemical Society unterstützt. (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 216-218 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    URL: http://dx.doi.org/10.1002/ange.19720840513
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    Die Photochemie von Oxiden aromatischer Amine (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 220-220 
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    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    URL: http://dx.doi.org/10.1002/ange.19720840517
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    Die EPR-Spektroskopie als Methode zur Konformationsanalyse (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 223-223 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    URL: http://dx.doi.org/10.1002/ange.19720840521
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    Gmelin-Durrer: Metallurgie des Eisens. Vierte. völlig neu bearbeitete Auflage, fortgeführt von G. Trömel und W. Zischkale. Ergänzung zu Gmelins Handbuch der anorganischen Chemie. 8. Auflage, System-Nr. 59, „Eisen“. Teil A. Lieferung 3 bis 5, Verlag Chemie GmbH, Weinheim/Bergstr. 1971. Band 3: Hochofen, Teil 1. Hochofenanlage. Hochofenbetrieb. Roheisen und Nebenprodukte. Stand der Roheisenerzeugung in der Welt. Band 3a: Textteil, VI, VII, 320 S., Band 3b: Figurenteil, VI. I. 137 S., 304 Abb., Ln. zusammen DM 487.- (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 225-225 
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    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    URL: http://dx.doi.org/10.1002/ange.19720840524
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    Aminosäure-Analyse im Picomol-BereichDiese Arbeit wurde von der Deutschen Forschungsgemeinschaft durch Sechmittel und ein Ausbildungs-Stipendium für N.L. unterstützt. (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 255-257 
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    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Stereochemie der Cycloaddition von 1,3-Diphenyl-2-azallyllithium an Alkene bei 0°CDiese Arbeit wurde von der Deutschen Forschungsgemeinschaft und dem Fonds der Chemischen Industrie unterstützt. (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 261-262 
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    Keywords: Chemistry ; General Chemistry
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    Reaktionen und Verbindungen mit molekularem Stickstoff (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 267-267 
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    Keywords: Chemistry ; General Chemistry
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    Die Blitz-Vakuum-Thermolyse von p-Benzochinonen (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 314-314 
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    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Synthese neuer DiazepinoneDie Diazepinone (1) könnten auch als 5,6-Dihydro-4H-1,2-diazepin-3(2H)-one, als 7-Oxo-4,5,6,7-tetrahydro-1 H-1,2-diazepine oder als 1,2-Diaza-7-cyclohepten-3-one bezeichnet werden. (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 153-154 
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    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Darstellung von cyclo-Octaschwefeloxid, S8O (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 344-345 
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    Keywords: Chemistry ; General Chemistry
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    cis,cis,trans-Tetracyclo[6.1.0.02,4.05,7]nonan, „trans-Trishomobenzol“ (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 346-347 
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    Keywords: Chemistry ; General Chemistry
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    Trifluormethansulfonsäure-carbonsäure-anhydride, hochwirksame Acylierungsmittel (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 294-295 
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    Keywords: Chemistry ; General Chemistry
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    Polymere Wittig-ReagentienDiese Arbeit wurde von der Deutschen Forschungsgemeinschaft. dem Fonds der chemischen Industrie und der Fa. E. Merck unterstützt. (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 296-297 
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    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Kationen und Radikale aus Cyclopropanon-Addukten (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 303-304 
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    Keywords: Chemistry ; General Chemistry
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    Dimerisierung von optisch aktivem 1,3-Diphenylallen (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 304-304 
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    Keywords: Chemistry ; General Chemistry
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    Cycloadditionen und Umlagerungen von 2-Oxyallyl-Systemen (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 305-306 
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    Reaktivierung phosphorylierter Acetylcholin-Esterase  -  Abhängigkeit von der Aktivator-Acidität (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 354-356 
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    Keywords: Chemistry ; General Chemistry
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    Chemistry of acetylenes. Von H. G. Viehe. Marcel Dekker Inc., New York 1969. 1. Aufl., XV, 1298 S., zahlr. Tab. u. Formeln, geb. $ 59.50 (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 367-367 
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    Computer in der chemischen Forschung  -  ein Vorwort (1972)
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    Zeitschrift für die chemische Industrie 84 (1972), S. 369-371 
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    Die Anwendung einer Rechenanlage in der Praxis der kernmagnetischen Resonanzspektroskopie (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 400-411 
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    Totalsynthese von [α,γ-14C2]-Uroporphyrin III (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 432-433 
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    Handbook of Chemistry and Physics. Von R. C. Weast. The Chemical Rubber Co., Cleveland, Ohio/USA 1972. 52. Aufl., XXVII, 2313 S., geb. DM 99.80 (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 445-446 
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    Die Funktion des Metall-Ions in der Carboanhydrase (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 461-471 
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    Notes: Im vorliegenden Fortschrittsbericht soll eine Zusammenfassung über die Chemie des Metall-Ions im Enzym Carboanhydrase gegeben werden. Der Schwerpunkt liegt dabei auf der Ableitung der Wirkungsweise des Enzyms aus dem Studium des Metalls. Die Ergebnisse aus der Chemie der Metallenzyme scheinen eine Neubewertung einiger Aspekte der herkömmlichen Metallkomplex-Chemie zu verlangen.
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    Synthese stabiler 1-Benzothiepine (1972)
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    Zeitschrift für die chemische Industrie 84 (1972), S. 477-478 
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    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Neue Wege zu Quadratsäure-1,3-diamidenDiese Arbeit wurde vom Fonds der Chemischen Industrie, der DECHEMA und der Badischen Anilin- & Sodafabrik AG unterstützt sowie von den Chemischen Werken Hüls durch Überlassung von Quadratsäure-Derivaten. (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 480-481 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Rundschau (1972)
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    Zeitschrift für die chemische Industrie 84 (1972), S. 486-487 
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    Impressum (1972)
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    Zeitschrift für die chemische Industrie 84 (1972) 
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    DTA-Untersuchungen zur Bildung von Dehydrobenzol und Dichlorcarben aus Halogenorganolithium-Verbindungen (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 527-528 
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    Photochemische Oxygenierung von 2,4,6-Tri-tert.-butyl-λ3-phosphorin und 2,4,6-Tri-tert.-butyl-1,1-dimethoxy-λ5-phosphorinHerrn Professor G. Wittig in dankbarer Verehrung zum 75. Geburtstag gewidmet. (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 526-527 
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    Neuere nichtsteroidartige entzündungshemmende Wirkstoffe (Antiphlogistica) (1972)
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    Zeitschrift für die chemische Industrie 84 (1972), S. 512-526 
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    Notes: Unter den neueren nichtsteroidartigen Antiphlogistica haben Analoga des Indomethacins, der Phenylessigsäure und der Heteroarylessigsäuren das Stadium der klinischen Prüfung erreicht. Ihre biochemische Wirkungsweise wird am Beispiel von Indomethacin beschrieben, welches Hemmwirkungen auf Entzündungssubstanzen und Enzyme sowie Einflüsse auf die Zellmembran ausübt und die Prostaglandin-Biosynthese hemmt. Die Bedeutung der pharmakodynamischen Eigenschaften für die klinischen Wirkungen konnte an einigen Beispielen gezeigt werden. Mehrere Prüfsubstanzen schieden wegen ihrer Nebenwirkungen aus. Einige α-Methyl-arylessigsäuren zeigten hinsichtlich Wirkungsstärke und Metabolismus in vivo sowie der Hemmung der Prostaglandin-Synthetase und der Albumin-Bindung in vitro hohe Stereospezifität. Mit dem induzierten Cotton-Effekt steht eine empfindliche Methode zur Untersuchung der Wechselwirkungen zwischen Wirkstoffen und Biopolymeren zur Verfügung. Kompetitive Bindungen und antagonistische Wechselwirkungen zwischen den nichtsteroidartigen Wirkstoffen, speziell bei Acetylsalicylsäure (Aspirin) wurden sowohl in vitro als auch in vivo beobachtet. Ein Fortschritt auf dem Gebiet der Salicylsäure-Derivate war die Synthese des stärker und länger wirkenden Flufenisals. Analoga der Fenamate und mehrere neuartige chemische Verbindungen zeigten vielversprechende Eigenschaften in ersten klinischen Versuchen. Einige immunologische Methoden zur Behandlung der rheumatoiden Arthritis werden z. Z. untersucht. Für eine wirksamere Bekämpfung der arthritischen Erkrankungen sind jedoch neue Konzepte erforderlich.
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    Struktur und Bildungsweise eines neuen phosphorhaltigen Heterocyclus; Länge der P - Cl-Bindung und 35Cl-NQR-FrequenzWir danken den folgenden Institutionen für finanzielle Unterstützung: dem S. R. C. für T. S. C. und K. D. H.), dem Henry-Lester-Trust (für C. Y. C.) und dem Türkischen Forschungsrat (für T. D.).Herrn Professor G. Wittig zum 75. Geburtstag gewidmet (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 530-531 
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    Ein alternativer Ansatz zur Nomenklatur cyclisch konjugierter Polyolefine einschließlich einiger Bemerkungen über den Gebrauch des Ausdrucks „aromatisch“ (1972)
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    Zeitschrift für die chemische Industrie 84 (1972), S. 447-451 
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    Keywords: Chemistry ; General Chemistry
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    Notes: Der Gebrauch des Wortes „aromatisch“ hat zu so viel Unklarheit und Meinungsverschiedenheit geführt, daß er vielleicht mit Vorteil eingestellt würde.
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    Struktur uod Funktion chromosomaler Nucleohistone (1972)
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    Zeitschrift für die chemische Industrie 84 (1972), S. 451-461 
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    Notes: Durch die Fortschritte der molekularen Biologie ist eindeutig bewiesen, daß alle genetischen Informationen zellulärer Organismen in der DNA enthalten sind. Der Hauptanteil der DNA ist in den Zellkernen lokalisiert und bildet mit Histonen und sauren Proteinen das Grundgerüst der Chromosomen. Dieser DNA/Protein-Komplex, auch Nucleohiston oder Chromatin genannt, repräsentiert die Anzahl aller vorhandenen Gene eines Zelltypes und ist eine genetisch funktionelle Einheit. Allen genetischen Strukturen ist dieses Grundelement gemeinsam, und nur dessen räumliche Anordnung oder Multiplizität bestimmt die artgebundene unterschiedliche Morphologie der Chromosomen. Als strukturerhaltende Elemente können die Histone angesehen werden, die im allgemeinen mit dichtgepackten und metabolisch inaktiven DNA-Sequenzen assoziiert sind. Das lysinreiche Histon I scheint eine spezielle regulatorische Funktion zu erfüllen, da es die Transkription bestimmter Gene zu unterdrücken vermag. Die sauren Proteine sind vor allem in metabolisch aktiven chromosomalen Strukturen nachweisbar, die gestreckte DNA-Sequenzen aufweisen und den Zusammenhang zwischen Struktur und Funktion der Nucleohistone besonders unterstreichen. Ungefähr 50% der DNA im Nucleohiston-Komplex sind nicht durch Proteine maskiert, obwohl nur ein geringer Prozentsatz dieser freien Nucleotidsequenzen durch RNA-Polymerase transkribiert werden kann. Da die bis jetzt sequenzanalytisch untersuchten Histone eine ungleiche Verteilung der basischen Aminosäurereste aufweisen und nur durch diese geladenen Sequenzen an die DNA gebunden werden können, ist diese “offene” Struktur leicht erklärlich. In dem von Crick vorgeschlagenen Chromosomen-Modell wird die einzigartige Struktur der Histone berücksichtigt und angenommen, daß die histonreichen Heterochromatinbänder nur multiple Kontrollelemente enthalten und nicht transkribiert werden. Dagegen soll das Euchromatin funktionellen Genen entsprechen, die bereits einsträngige DNA enthalten und für diese spezifische Konformation besondere Proteine benötigen.
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    Aza[17]annuleneHerrn Professor R. Criegee zum 70. Geburtstag gewidmetDiese Arbeit wurde von der Badischen Anilin- & Soda-Fabrik AG sowie der Deutschen Forschungsgemeinschaft unterstützt. (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 474-475 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    α-Isocyan-γ-cyan-alkansäure-äthylester aus α-metalliertem Isocyanessigsäure- oder -propionsäure-äthylester und Acrylnitrilen (1972)
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    Zeitschrift für die chemische Industrie 84 (1972), S. 478-479 
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    Cyclodimerisation von Methylencyclopropan in der Hitze (1972)
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    Zeitschrift für die chemische Industrie 84 (1972), S. 483-483 
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    Keywords: Chemistry ; General Chemistry
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    URL: http://dx.doi.org/10.1002/ange.19720841015
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    Über einige neue stickstoffhaltige Redoxpolymere (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 361-361 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19720840830
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    Die Analyse enger Molekulargewichtsverteilungen als Hilfsmittel zur kinetischen Erforschung ionischer Polymerisationsprozesse (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 362-363 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ange.19720840834
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  • 93
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    Über die Anwendung von enzymanalog gebauten Polymeren zur Racemattrennung (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 364-364 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ange.19720840838
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  • 94
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    Die Photoelektronen-Spektren der benzenoiden Kohlenwasserstoffe C18H12 (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 551-553 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/ange.19720841127
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    Abschätzung der Spirowechselwirkung (Spirokonjugation) in Tetravinylsilan (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 551-551 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19720841126
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  • 96
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    Analytical Flame Spectroscopy - Selected Topics. Herausgeg. von R. Mavrodineanu. Macmillan and Co. Ltd., London-Basingstoke 1970. 1. Aufl. XXII, 772 S., zahlr. Abb. u. Tab., geb. £ 14. -  (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 555-556 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ange.19720841131
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    Regeln und Steuern. Eine Einführung für Chemiker und Ingenieure. Von A. Schöne. Verlag Chemie GmbH, Weinheim/Bergstr. 1971, 1. Aufl., VIII, 207 S., 136 Abb., 8 Tab., geb. DM 33. -  (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 554-555 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    URL: http://dx.doi.org/10.1002/ange.19720841130
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  • 98
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    Optisch aktive Molybdän- und Wolfram-Komplexe mit tetragonal-pyramidaler Konfiguration (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 442-443 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19720840918
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    Impressum (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972) 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ange.19720841001
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  • 100
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    Einfache Synthese von [2.2]Paracyclophanen (1972)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 84 (1972), S. 471-472 
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    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19720841005
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