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  • Articles  (159)
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  • Articles: DFG German National Licenses  (159)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Colloid & polymer science 260 (1982), S. 564-569 
    ISSN: 1435-1536
    Keywords: lin. Polyethylene ; Single crystals ; Heat of Fusion ; DSC ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Recently published results for solution crystallized PE single crystals have shown, that the experimental heat of fusionΔH * is higher, if the solvent is exchanged to silicon oil (oil suspension samples) as compared with dried mats. This has been interpreted by the collapse of the original hollow pyramids during drying, inducing lateral defects within the lamellae. The present investigation does not confirm this unexpected result.ΔH * of dried mats (T c 66 to 91 °C) and of the corresponding oil suspension samples agree within the rather small limits of experimental error. The crystallinities as derived fromΔH *, density or WAXS are in excellent agreement. SEM micrographs of cold fractured dried mats show their spongy macromorphology, but TEM micrographs of stained ultra-thin sections reveal the lamellar morphology of the walls, consisting of curved lamellae and stacked hollow pyramides. If a dried mat is sintered at room temperature, a dense transparent film is obtained with a rather regular stacked morphology of large flat lamellae.ΔH * of these films agrees with that of the original mat.
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  • 2
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    Calcified tissue international 33 (1981), S. 143-151 
    ISSN: 1432-0827
    Keywords: Calcium transport ; Cytochalasin B ; Dihydrocytochalasin B ; Colchicine ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary In vivo calcium absorption was studied in normal and rachitic chicks. Cytochalasin B (CB) at a concentration of 25 µg/ml added to the medium inside the duodenal lumen inhibited calcium absorption (20 min) from 82.5±1.9% of calcium absorbed in the controls to 59.2±3% in normal and from 70.0±2.3% to 47.0±2.1% in rachitic chicks. In vitro studies by everted ileal sacs of young rabbits also showed an inhibition of active transport of calcium due to CB. Whereas in the controls the ratio of45Ca concentrations in serosal and mucosal media (60 min) was 7.2±0.32, the ratios were 5.24±0.52; 4.40±0.36; 3.40±0.42; 5.77±0.52; 1.38±0.08; and 1.06±0.02 in the presence of CB at concentrations of 5, 10 and 25 µg/ml; colchicine 10−4M, Na citrate 0.02M, and heat-devitalized conditions, respectively.45Ca concentration in the mucosal scrapings was also affected. It showed an increase from controls (15,101±404 cpm/mg) and correlated with CB concentration: 17,378±489, 19,015±1000, and 20,201±362 at 5, 10, and 25 µg/ml, respectively. Dihydrocytochalasin B also inhibited active calcium transport and caused an increase in45Ca concentration in the mucosal scrapings. Correlated electron microscopic studies showed certain changes in the brush border, especially in some actin microfilaments in the terminal web region. It seems that these morphological alterations may be related to transcytoplasmic movement of calcium.
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  • 3
    ISSN: 1432-0827
    Keywords: Alcohol ; Electron microscopy ; Growth plate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary We have previously demonstrated that ethanol has a direct toxic effect on the rat skeleton characterized by decreased trabecular bone volume. In the present study, we examined the ultrastructure of the distal radial epiphyseal growth plates in these same animals. Eight weeks of ethanol administration to 12 male rats results in serum alcohol levels of 140 mg/dl but did not alter the width or light microscopic appearance of the radial growth plate. Quantitative electron microscopy failed to demonstrate morphologic evidence of toxicity in the skeletal cells. We conclude that although ethanol appears to have a direct effect on rat bone characterized by enhanced resorption, toxicity is not attended by ultrastructural changes in the skeletal cells.
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  • 4
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    Calcified tissue international 33 (1981), S. 529-540 
    ISSN: 1432-0827
    Keywords: Bone cells ; Electron microscopy ; PTH ; PGE1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Four different cell populations—designated PF, OB, OC, and PC—were isolated from calvaria of 18-day-old chick embryos for analysis of the effects of hormones on bone tissue. The cell populations were studied with histological and biochemical methods. Apart from the well-known cell types present in calvaria, a new cell type was found in the noncalcified organic matrix between the osteoblastic layer and the calcified matrix. These cells were provisionally called osteocytic osteoblasts. They represent the “transition state” between osteoblasts and osteocytes. On the basis of histological studies with light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM), the PF population was considered to originate primarily from the periosteal fibroblasts, the OB population from the osteoblasts and osteocytic osteoblasts. The population of cells still present in calvaria after removal of periosteal fibroblasts and osteoblasts was called the OC population. This cell population was very much enriched with osteocytes. The fourth isolated population (PC) was a mixed population of fibroblasts, osteoblasts, and preosteoblasts. On exposure to parathyroid hormone (PTH), all four cell populations showed increased lactate production, but only the OB and OC populations displayed increased cAMP production. Prostaglandin E1 (PGE1) stimulated cAMP production in both OB and PF cells. From the results of this study it was concluded that PTH receptors are present on all of the cell types studied, but that occupancy of the receptor induces adenylate cyclase stimulation only in osteocytes and fully differentiated osteoblasts.
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  • 5
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    Calcified tissue international 30 (1980), S. 43-50 
    ISSN: 1432-0827
    Keywords: Osteogenesis ; In vitro ; Electron microscopy ; Mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Chick limb mesenchymal cells differentiate into muscle, cartilage, fibrous, and bone tissue. Previous reports show that when stage 24 limb mesenchymal cells are cultured in vitro, chondrocytes, myocytes, fibrocytes, and osteoblasts can be identified on the basis of morphological and biochemical parameters. The study reported here demonstrates that phenotypic expression in culture seems to be dependent on the initial plating density, Scanning electron microscopic observations indicate that when stage 24 limb mesenchymal cells are initially seeded at high densities (5 × 106 cells per 35 mm culture dish), mounds of cells appear in culture. These mounds represent cartilage nodules composed of a fine fibrous matrix and chondrocytes, surrounded by a loose fibrous connective tissue matrix. Cultures initially plated at intermediate densities (2.0–2.5 × 106 cells/35 mm culture dish) produce a flattened layer of fibrocytes overlying a matrix of collagen fibers and calcium phosphate deposits as determined by electron-microprobe analysis; these observations are indicative of osteoblast expression. Cells seeded at this intermediate density appear larger and possess greater surface area than cells seeded at high density. It is suggested that conditions that permit such increased cell surface area coupled with a relative compaction due to cell crowding may provide conditions permissive for osteogenesis. Based on morphological criteria, it appears that chick limb mesenchymal cell osteogenesis in vitro is not associated with chondrogenesis but represents a separate route of phenotypic expression.
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  • 6
    ISSN: 1432-0827
    Keywords: Rat ; Calvarium ; Electron microscopy ; Preosteoclasts ; Osteoclasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary This is a study of the fine structure of cells of the 20-day fetal rat calvarium. Special attention is given to identifying and characterizing preosteoclasts. These cells are relatively common and located largely, but not exclusively, at the endocranial bone surface. The preosteoclasts are characterized by abundant mitochondria, an incomplete perinuclear Golgi apparatus, and variable-shaped dense granules. The dense granules are unique in appearance in that they contain an internal dense matrix surrounded by a clear halo. Most granules are circular in shape but some are elongate or tubular in form. Granules with identical appearance are observed in osteoclasts. The preosteoclasts are mononucleate, or occasionally binucleate. It is suggested that because preosteoclasts are morphologically distinctive and relatively abundant, it should be feasible to separate these cells from a heterogeneous cell isolate.
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  • 7
    ISSN: 1432-0827
    Keywords: Decalcification ; Electron microscopy ; Bone matrix ; Bone glycoproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary A solution of triethylammonium EDTA in 80% ethanol was evaluated as a demineralizing reagent for bone in comparison with aqueous solutions of EDTA. Biochemical analysis and acrylamide gel electrophoresis of extracts of finely powdered bovine bone showed that most of the macromolecular components of the organic matrix extractable in aqueous EDTA were retained when the triethylammonium EDTA reagent was used. Ultrastructural examination of chick tibias decalcified with the reagents showed a better preservation of cellular morphology, especially the membranous components, and more uniformly distributed ground substance, though slightly less in quantity, when the aqueous reagent was used. Use of the two reagents appears to be complementary, the alkylammonium reagent being more appropriate for use in studies of the organic matrix of bone, including immunohistochemical studies of bone glycoproteins. The aqueous reagent is more appropriate for use in studies of cellular ultrastructure.
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  • 8
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    Calcified tissue international 33 (1981), S. 119-124 
    ISSN: 1432-0827
    Keywords: Avian eggshell ; Microstructure ; Electron microscopy ; Electron diffraction ; Calcite growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The eggshell of the domestic fowl has been studied by transmission electron microscopy and diffraction. Thin sections of shell were prepared by chemical and ion-beam thinning techniques. Each calcite column of the palisade layer consisted of crystallites of diameter 20 to 30 µm with some tendency for crystallite alignment within a single column. Evidence indicates that there was no significant preferred orientation in the palisade layer as a whole. Only in the surface layer was any preferred orientation detected, and here {1014} planes tended to lie parallel to the surface. The results are compared with previously published data, and calcite nucleation and growth are discussed.
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  • 9
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    Journal of nondestructive evaluation 1 (1980), S. 277-286 
    ISSN: 1573-4862
    Keywords: Ultrasonics ; through-transmission ; composite materials ; attenuation ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The steady-state amplitude of the output of an ultrasonic through-transmission measurement is analyzed and the result is given in closed form. Provided that the product of the input and output transduction ratios, the specimen-transducer reflection coefficient, the specimen-transducer phase-shift parameter, and the material phase velocity are known, this analysis gives a means for determining the through-thickness attenuation of an individual thin sample. Multiple stress-wave reflections are taken into account, and so signal echoes do not represent a difficulty. An example is presented for a graphite fiber epoxy composite (Hercules AS/3501-6). Thus, the technique provides a direct method for continuous or intermittent monitoring of through-thickness attenuation of plate structures which may be subject to service structural degradation.
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  • 10
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    Journal of nondestructive evaluation 2 (1981), S. 57-63 
    ISSN: 1573-4862
    Keywords: ultrasonics ; correlation ; pseudorandom codes ; random signals ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract A new portable digital random signal flaw detection system is described which uses a digital delay line to replace the acoustic delay line of the original random signal system. Using this new system, a comparison was made between the two types of transmit signals which have been used in previous systems—m-sequences and random signals. This comparison has not been possible with these previous correlation flaw detection systems. Results indicated that for high-speed short code operation, the m-sequences produced slightly lower range sidelobes than typical samples of a clipped random signal. For normal long code operation, results indicated that system performance is essentially equivalent in resolution and signal-to-noise ratio using either m-sequences or clipped and sampled random signals. Further results also showed that for normal long code operation, the system produces outputs equivalent in resolution to pulse-echo systems, but with the added benefit of signal-to-noise ratio enhancement.
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  • 11
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    Journal of nondestructive evaluation 2 (1981), S. 85-111 
    ISSN: 1573-4862
    Keywords: Holographic reconstruction ; ultrasound ; NDE ; Rayleigh-Sommerfeld formula
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract For the purpose of ultrasonic nondestructive testing of materials, holography in connection with digital reconstruction algorithms has been proposed as a modern tool to extract crack sizes from ultrasonic scattering data. Defining the typical holographic reconstruction algorithm as the application of the scalar Kirchhoff diffraction theory to backward wave propagation, we demonstrate its general incapability of reconstructing equivalent sources, and hence, geometries of scattering bodies. Only the special case of a planar measurement recording surface, that is to say, a hologram plane, and a planar crack with perfectly rigid boundary conditions parallel to the hologram plane and perpendicular to the incident field yields a nearly perfect correlation between crack size and reconstructed image; the reconstruction algorithm is then referred to as the Rayleigh-Sommerfeld formula; it therefore represents the optimal case matched to that special geometrical situation and, hence, may be interpreted as a quasi-matched spatial filter. Using integral equation theory and physical optics, we compute synthetic holographic data for a linear cracklike scatterer for both plane and spherical wave incidence, the latter case simulating a synthetic aperture impulse echo situation, thus illustrating how the Rayleigh-Sommerfeld algorithm or its Fresnel approximation increasingly fail for cracks inclined to the hologram plane and excited nonperpendicularly. Furthermore, we point out how the physical data recording process may additionally influence the reconstruction accuracy, and, finally, guidelines for a careful and serious application of these holographic reconstruction algorithms are given. The theoretical results are supported by measurements.
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  • 12
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    Journal of nondestructive evaluation 2 (1981), S. 203-207 
    ISSN: 1573-4862
    Keywords: ultrasonics ; synthetic aperture ; spatial frequency ; interior imaging ; acoustic microscopy ; defect detection ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract A new acoustic synthetic aperture geometry is demonstrated in which the image field is sampled in the spatial frequency domain. This means the sampled field is recorded in a particularly convenient form for presentation to the back propagation algorithms used to reconstruct the field at the object plane. The method has applications in interior visualization. It is difficult to image the interior of solid objects using lens based imaging systems because a different lens geometry must be used for each distinct object material. The system presented here overcomes such problems since both the angular variation of the transmission coefficient at the object-water interface and the aberrations introduced by the velocity mismatch at the object surface may be readily compensated for in the back propagation routine. Experimental results are presented illustrating the detection of four half wavelength diameter defects, spaced by three wavelengths, at a depth of eight wavelengths below the surface of an aluminum block.
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  • 13
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    Journal of nondestructive evaluation 2 (1981), S. 241-247 
    ISSN: 1573-4862
    Keywords: ultrasonic field ; numerical methods ; transducer design ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract A simple, flexible, accurate, and comprehensive numerical method is presented for theoretically analyzing the diffraction field of a continuous wave transducer of arbitrary size, shape, and frequency. Using the extensively studied circular transducer for comparison, numerical results are shown for an unfocused transducer with uniform velocity excitation as well as for a focused transducer with Gaussian velocity excitation. Data concerning the execution time, program size, and convergence of the method are also presented for its implementation as a design tool on a minicomputer system.
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  • 14
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    Journal of nondestructive evaluation 3 (1982), S. 55-63 
    ISSN: 1573-4862
    Keywords: ultrasonics ; Rayleigh waves ; subsurface defects ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract An ultrasonic inspection method is used to obtain the circumference of a subsurface hole and the depth of the hole below the surface. A pitch-catch Rayleigh wave transducer set-up was used to launch a Rayleigh surface wave at the flaw and to capture and record the scattered waves. The frequency spectrum of the scattered waves can be used to obtain the depth of the hole. The ligament of material between the hole and the surface is sent into resonance, and this feature can be extracted from the scattered waves' frequency spectrum. The frequency is a function of the ligament length; thus the hole depth can be obtained. The circumference of the hole is found from a time of flight measurement. A Rayleigh wave is formed that travels around the hole's surface. The length of time required for the wave to travel around the hole is a measure of the circumference.
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  • 15
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    Journal of nondestructive evaluation 3 (1982), S. 65-76 
    ISSN: 1573-4862
    Keywords: crack measurement ; ac field technique ; arbitrary skin depth ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract A general solution is given to describe the ac field around surface-breaking cracks for arbitrary values of the ratio of skin depth δ to crack depthd. This solution allows accurate interpretation of crack depth from voltage readings taken by a Crack Microgauge instrument in cases where δ andd are comparable. It is shown to agree with asymptotic formulae obtained for the cases where δ/d is very small and very large. Provided that the probe length Δ is large compared with δ, the calculations show that a very good approximation to the crack depth may be obtained, irrespective of the ratio δ/d, by using the thin-skin asymptotic formula $$d = \frac{1}{2}\Delta (V_2 - V_1 )/V_1 $$ whereV 1 andV 2 are probe voltages registered at positions just off and across the crack, respectively. The problem is solved for a crack of infinite aspect ratio which has no opening, but it can be applied with confidence at the centerline of cracks of fairly large aspect ratio.
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  • 16
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    Journal of nondestructive evaluation 3 (1982), S. 99-109 
    ISSN: 1573-4862
    Keywords: thermography ; infrared scanning ; calibration ; inverse problems ; cavity detection ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract An approach for treating nondestructive testing as the solution of inverse problems in mathematical physics has been used for the detection of cavities. The approach is developed based on the use of an additional boundary condition of scanned temperature on the surface to solve for the cavity geometry. For the present study, the condition at the cavity side is taken to be that of a specified temperature, and the experiment is carried out to meet this condition. Two specimens are tested in this paper, a plane slab and a rectangular prism. In both bodies the cavity is rectangular in shape. For the testing of the plane slab, the method is able to detect the cavity wall with high accuracy, whereas the cavity depth error is larger (6%). The detection of the cavity position in the rectangular prism has an error ranging from −9.7 to 7.7%. Errors in the experiment are attributed to the uncertainties in the measurements of temperature and the Biot number. The former is read off from the analog data output of the infrared scanner. The latter is not measured separately, but is computed from the scanned data and thus becomes a portion of the total nondestructive testing output. A final note is also made in this paper to relate how the presented method can be used in actual practice.
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  • 17
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    Journal of nondestructive evaluation 3 (1982), S. 175-182 
    ISSN: 1573-4862
    Keywords: ultrasonic wave scattering ; inclusions ; reciprocity ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract This paper, as part of a series on elastic wave scattering, presents results of measurements and calculations on scattering of ultrasonic waves by a solid spherical inclusion (tungsten carbide) embedded in titanium alloy by the diffusion bonding process. Both direct scattering and mode-converted scattering angular distributions are reported for shear and compressional incident waves. The consequences upon the signals when transmitter and receiver were interchanged are explored in a reciprocity rule.
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  • 18
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    Journal of nondestructive evaluation 2 (1981), S. 23-33 
    ISSN: 1573-4862
    Keywords: austenitic stainless steel ; austenitic stainless steel welds ; eddy current inspection ; eddy current testing ; electromagnetic testing ; ferromagnetic steel inspection ; flaw characterization ; flaw detection ; NDE ; multifrequency eddy current inspection ; weld inspection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract This paper describes the initial phase of a project to develop eddy-current methods to inspect welds joining sections of austenitic stainless steel pipe having walls up to 13 mm (0.5 in.) thick. The objective of this phase was to demonstrate the feasibility of detecting and characterizing flaws in austenitic stainless steel base metals. These materials and welds present challenging eddy-current problems because of their relatively large thickness and ferromagnetism. Multiparameter analysis shows that a reflection coil probe operated with three discrete driver frequencies and phase detection can locate and size a cracklike defect in a single conductor in the presence of variations in conductor resistivity, permeability, and thickness and in the probe-conductor spacing (liftoff). Experiments were performed with a modular three-frequency instrument. Flat-plate specimens of types 304L and 347 stainless steel machined to 12.7 to 15.9 mm thickness simulated pipe walls; saw-cut slots 10 to 30% of nominal specimen thickness simulated cracklike defects. The same slots were used in duplicate experiments as near-side (directly under the test probe) or far-side (in the face opposite the probe) defects. Flaw detection and characterization capability was demonstrated by a series of experimental measurements fitted to specimen properties by least squares techniques. The quality of the fit determined the expected accuracy of measurement. Comparison of accuracy estimates determined the best choice of operating frequencies. From the 1,2,5 sequence of frequencies between 0.5 and 20 kHz, the optimum set of operating frequencies was selected to be 0.5, 2, and 10 kHz. Estimates of measurement accuracy for combined near- and far-side defect cases were: plate thickness, 0.74 mm; probe liftoff, 0.03 mm; defect location (depth of material above defect), 3.48 mm; and defect size (vertical slot depth), 1.09 mm. A few property values were back-calculated from instrument readings; the errors in these values were somewhat larger than the measurement accuracy estimates because of instrument drift and the absence of calibration circuits.
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  • 19
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    Journal of nondestructive evaluation 1 (1980), S. 263-276 
    ISSN: 1573-4862
    Keywords: Ultrasonics ; scattering ; diffraction ; defect characterization ; cracks ; voids ; pattern recognition ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Shape and size are the most important defect characteristics that need to be determined for the reliable prediction of the remaining service lifetime of a defective structure or part. The analytical and supporting experimental results presented in this paper concern a general, pattern recognition-based, ultrasonic defect identification and sizing method. The satellite-pulse technique (SPT) is based on the interpretation, in terms of defect types (shapes) and dimensions, of the separation in time of arrival between the readily detected specularly reflected pulse and its generally ignored tip-diffracted or tangentially scattered “satellite” contained in the received waveform. Calibration procedures were also developed that enable the ultrasonic examiner to read the time scale of the oscilloscope for equivalent crack depth or void diameter as appropriate.
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  • 20
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    Journal of nondestructive evaluation 2 (1981), S. 35-41 
    ISSN: 1573-4862
    Keywords: acoustic emission ; crack growth ; 7075 aluminum ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract For slow crack growth (da/dn ≈0.1 µm per load cycle) in 7075-T6 aluminum, quantitative agreement was found between the amplitude distribution of burst acoustic emissions and the area size distribution of intermetallic inclusions ≈10 (µm)2 in area as measured on thepolished fracture face. This observation permits the prediction of the amplitude distribution of acoustic emissions due to crack growth in a particular sample of 7075-T6 aluminum directly from a simple, standard metallographic observation performed on the material in question. It was also found that a reduction of the yield stress from that of 7075-T6 aluminum (503 MPa) to that of 7075-0 aluminum (103 MPa) completely eliminated burst acoustic emission activity due to crack advance in the amplitude range studied.
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  • 21
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    Journal of nondestructive evaluation 2 (1981), S. 119-124 
    ISSN: 1573-4862
    Keywords: Stress intensity factor ; annular crack ; finite element methods ; fatigue ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Results of finite element static stress intensity factor calculations for an annular crack around a spherical inclusion (void) are presented and compared with those from approximate analytical methods.
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  • 22
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    Journal of nondestructive evaluation 2 (1981), S. 147-149 
    ISSN: 1573-4862
    Keywords: Ultrasonic tomography ; residual stress ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Ultrasonic tomography is being explored as a potential tool to map residual stresses in solids. The angular scanning required in tomography imposed limitations on the stress configuration adaptable to such a treatment. These are discussed in the following note.
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  • 23
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    Journal of nondestructive evaluation 2 (1981), S. 161-172 
    ISSN: 1573-4862
    Keywords: elastodynamic inverse scattering ; ultrasonics ; data reduction ; ill-posed problems ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The practical significance of ill-posedness in a data reduction problem is reviewed. Inverse elastodynamic scattering is shown to be ill-posed in general, although suitably restricted problems may be well-posed. These results underscore the need to analyze carefully the errors of data reduction problems in NDE, and to focus attention on final results of an NDE exercise, rather than on intermediate steps.
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  • 24
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    Journal of nondestructive evaluation 2 (1981), S. 153-159 
    ISSN: 1573-4862
    Keywords: ultrasonics ; scattering ; cracks ; periodic surface perturbation ; NDE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The scattering of a plane longitudinal wave from a two-dimensional crack, with a sinusoidal surface perturbation whose amplitude and wavelength are much smaller than the length of the crack, is investigated. The amplitude of the cylindrical body waves in the far field are calculated from a Kirchhoff approximation that utilizes the solution to the reflection from the sinusoidal surface profile of a semiinfinite solid. The results are compared to those for a flat crack, and conditions for significant differences of the amplitude as a function of the angle of observation are discussed. Characteristic changes in the scattered field produced by profiles with different amplitudes and periods are explained.
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  • 25
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    Journal of nondestructive evaluation 2 (1981), S. 195-202 
    ISSN: 1573-4862
    Keywords: ac potential difference ; fatigue crack measurement ; slots ; artificial flaws ; parasitic voltages ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The development of a successful and accurate instrument for measuring surface-breaking cracks in metals using the ac field technique has raised several interesting theoretical problems. Measurements with the instrument, known as the Crack Microgauge, do not rely on any prior calibration against an artificial flaw such as a saw-cut in a test block, but some users accustomed to such a calibration from other devices have nevertheless wished to use the instrument in that fashion and have in some instances reported erroneous results. In this paper, we develop a simple theory to explain and quantify this phenomenon. We calculate the parasitic voltages induced in the instrument probe due to the finite opening possessed by an artificial flaw, and we use these results to reinterpret the instrument readings. Controlled experimental measurements on artificial flaws of rectangular cross-section made in aluminum and in steel are found to be in good agreement with the theory. It is shown, however, that application of the theory requires additional information about the internal phase shift associated with the instrument. To enhance the accuracy of the theory, the effect of the corners of the artificial flaws was also considered, although it was not very influential in this work.
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    Journal of nondestructive evaluation 3 (1982), S. 19-24 
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    Keywords: ac potential difference ; eddy current ; fatigue crack measurement ; skin effect equation ; large skin depth ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract This paper considers the solution of the skin-effect or Helmholtz equation, ∇2ψ=k 2ψ, for the two-dimensional flow of a uniform alternating current perturbed by a plane crack of uniform depth. Herek is the ratio of crack depthd to skin depth δ. When the skin depth is large compared with the crack depth andk f 0, the quasi-static approach to this problem ignores the terms on the right-hand side and constructs solutions from Laplace's equation which are essentially dc solutions and are correct to orderk. In this paper we consider behavior near the limit when the skin depth is large and give solutions which are correct to orderk 2. In an example we relate the results to the interpretation of readings from an ac potential difference instrument applied to the measurement of surface-breaking cracks.
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  • 27
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    Journal of nondestructive evaluation 3 (1982), S. 93-98 
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    Keywords: bounded ultrasonic beam reflectivity ; Rayleigh angle ; beam profile ; surface defects ; nonspecular reflection ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The relationships between characteristics of elastic defects and nonspecular features of bounded ultrasonic beams reflected at the Rayleigh angle from a liquid-solid interface are investigated. The results can serve as a theoretical basis for interpretation of Rayleigh angle nonspecularly reflected beam profiles as characterization of localized surface elastic defects.
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  • 28
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    Journal of nondestructive evaluation 3 (1982), S. 125-136 
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    Keywords: magnetic particle inspection ; magnetic leakage fields ; finite difference method ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Experimental measurements of leakage fields from cylindrical defects were obtained in a geometry which permitted simultaneous measurement of the magnetic induction of the material. The results obtained are compared with calculations using a nonlinear finite difference method. Both the experiments and the calculations indicate that the magnitude of the leakage field continues to grow nearly in proportion with the applied field well into the saturation region of the magnetic material. The implications for magnetic particle inspection are discussed.
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  • 29
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    Journal of nondestructive evaluation 3 (1982), S. 45-53 
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    Keywords: Born approximation ; ultrasonic scattering ; NDE ; time domain
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    Notes: Abstract The time domain Born approximation for ultrasonic scattering from volume flaws in an elastic medium is described. Results are given both for the direct and the inverse problem. The time domain picture leads to simple intuitive formulas, which we illustrate by means of several simple examples. Particular emphasis is given to the front surface echo and its use in reconstructing the properties of the flaw.
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  • 30
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    Journal of nondestructive evaluation 1 (1980), S. 3-9 
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    Keywords: harmonic generation ; fatigue ; microcracks ; aluminum ; surface acoustic waves ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Harmonic generation of surface acoustic waves (SAW) is shown to be a useful tool for the detection of the initiation of surface cracks during fatigue of high strength aluminum alloys. A model that relates the length and density of microcracks to the amplitude of a SAW harmonic signal is described, and an associated coefficient of generation efficiency for the second harmonic amplitude is derived. A correlation between experimentally measured harmonic amplitude and remaining fatigue life is then demonstrated, which allows the mean remaining fatigue lifetime to be estimated to within 5% over the last 50% of the fatigue life for an Al 7075 alloy if the cyclic stress amplitude, but not the duration of fatigue, is known.
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    Journal of nondestructive evaluation 1 (1980), S. 11-19 
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    Keywords: ultrasonic diffraction ; fatigue cracks ; crack closure ; crack surfaces ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract This paper describes an ultrasonic diffraction technique for characterizing fatigue cracks. The angular field of energy scattered from a crack tip was computed. Using the theoretically predicted and experimentally verified optimum range of angles, we measured the crack profiles by the ultrasonic diffraction technique. Ultrasonic measurements agreed very well with direct destructive measurements. In addition, fatigue crack closure was detected and information on crack surfaces was obtained.
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    Journal of nondestructive evaluation 1 (1980), S. 21-36 
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    Keywords: ultrasonics ; surface defects ; defect depth ; deconvolution ; correlation ; NDE
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    Notes: Abstract In previous work by the authors,(1,6) it was demonstrated that the presence of near-surface defects could be detected reliably, even though the defect echo was contained within the near-surface echo. The algorithm consists of examining the variation in the composite (near-surface plus defect) response after it has been deconvolved from a near-surface response known to be defect-free. This paper presents two algorithms that have been developed subsequent to the work presented in ref. (6) for estimating thedepth of a near-surface defect, given that its presence has already been detected. One algorithm uses complex frequency domain techniques, and the other uses time domain analysis. Both procedures operate on the surface-plus-defect signal, using reference signals containing surface-only and defect-only responses. The defect signal is extracted from the composite signal. Defect depth is then computed from the time difference between the centers of the front-surface and extracted defect responses. A mean absolute depth error of 0.015 in. was obtained by applying the algorithms to experimental data containing depths from 0.020 to 0.130 in. below the near-surface.
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  • 33
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    Journal of nondestructive evaluation 1 (1980), S. 37-52 
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    Keywords: failure prediction ; accept/reject decisions ; ceramic materials ; ultrasonics ; fracture ; probabilistic model ; NDE
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    Notes: Abstract A general probabilistic method for reaching accept/reject decisions and failure prediction based on nondestructive evaluation procedures is described. The method is illustrated for ceramic materials that fail by the activation of microcracks located at void surfaces. The inspection procedure used for the analysis is the long wavelength ultrasonic method. The analysis indicates influences on the decision level and on the false-reject probability of variations in the signal-to-noise ratio and in the preexistent void population. The ultrasonic inspection is shown to exert a relatively minor influence on the false-reject probability, even for low signal-to-noise ratios, low stresses, and a widely dispersed void population, because of the intrinsic variability of the selected failure process. More encouraging results concerning the utility of NDE are anticipated to apply to other failure mechanisms in ceramics.
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  • 34
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    Journal of nondestructive evaluation 1 (1980), S. 53-66 
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    Keywords: ultrasonic transducers ; phased array ; ultrasonic imaging ; impulse response ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The present contribution provides a method for the derivation of analytical near- and far-field expressions for the pressure step-function response of nonuniformly excited ultrasonic transducers modeled as pistons of arbitrary shape in an infinite rigid baffle. Explicit inversion of the Laplace transformed ultrasonic field using Cagniard's idea results in elementary functions or definite integrals of elementary functions, depending on the assumed nonuniform aperture distribution. The basic signal distortion is illustrated in terms of three-dimensional space-time plots of the pressure unit step-function response of a rectangular uniform aperture. The corresponding results for a phased-array transducer are also given. In addition, we investigate the pressure profile on the axis perpendicular to a uniform rectangular piston and its horizontal radiation pattern for different bandwidths of the exciting impulse.
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  • 35
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    Keywords: stress fields ; microstructure ; J integral ; stress intensity factor ; harness acoustic velocity ; acoustic elasticity ; NDE
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    Notes: Abstract A very precise system for measuring two-dimensional velocity fields in solid samples has been used for nondestructive measurements of both externally applied and residual inhomogeneous stresses in solids,J integrals, stress intensity factors of cracks, and hardness of quenched steel. The longitudinal velocity measurement is based on precise determination of the propagation transit time through the stressed solid specimen using a small diameter, water-coupled acoutic transducer, which is scanned mechanically over the sample. Changes in velocity are then related to changes of stress in the sample by the theory of acoustoelasticity. Similar measurements show a high degree of correlation between longitudinal velocity changes and changes in microstructure in steel samples. Applications to problems of solid mechanics and material science illustrate the utility of this nondestructive measuring technique.
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  • 36
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    Journal of nondestructive evaluation 1 (1980), S. 79-85 
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    Keywords: ultrasonic waves ; EMAT ; surface stress ; NDE ; Green's function
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    Notes: Abstract An analysis of the radiation from a body force shows that it is equivalent to the radiation from a series of surface stresses defined by the moments of the body force taken with respect to the depth coordinate. As the body force becomes localized near the surface, the zeroth moment of the force dominates the radiation and is often thought of as an equivalent surface stress. However, under certain conditions, this can vanish, and the other moments must be considered. It is found that, as the order of the moment of a particular force component increases, the resulting radiation patterns alternate between those characteristic of a compressive surface stress and those characteristic of a shear surface stress, which have considerably different angular variations. Results of experiments in the development of EMAT transducers for nondestructive testing that support these results are cited, and important consequences in the design of inspection systems are indicated.
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    Journal of nondestructive evaluation 1 (1980), S. 225-233 
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    Keywords: Acoustic emission ; energy analysis ; composite materials ; strength ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract A new technique which uses the output of a true RMS voltmeter to measure the acoustic emission energy output of a transducer is presented. To demonstrate its use in a typical case, this procedure is used to measure acoustic emission energy during tensile tests on [0°/±30°/90°]s glass-epoxy laminate uniaxial and 10° off-axis tensile coupons. The test results were compared with numerical predictions of laminate response and acoustic emission energy. The experiments indicate that acoustic emission energy can be used to indicate the onset of ply and interlaminar failure.
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    Journal of nondestructive evaluation 1 (1980), S. 235-247 
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    Keywords: Ultrasonics ; acoustic waves ; scattering ; diffraction ; defect characterization ; silicon nitride ; NDE
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    Notes: Abstract The scattering of acoustic waves by different types of spherical defects in a silicon nitride matrix is calculated by using Ying and Truell's scattering theory. The theoretical scattering results are interpreted using a ray tracing approach. Experiments were carried out at a high frequency (150–450 MHz) to characterize defects in silicon nitride. Time and space averaging, Wiener filtering, diffraction, and propagation loss corrections were used to remove the effect of the transducer response and propagation loss in the material from the scattered signal. Our experimental results indicate the presence of a new type of defect in silicon nitride. They give the type and size of voids, cracks, and Si inclusions in good agreement with measurements obtained after sectioning.
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    Journal of nondestructive evaluation 3 (1982), S. 183-188 
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    Keywords: Ultrasonics ; Born inversion ; NDE
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    Notes: Abstract Quantitative reconstruction of volumetric flaws near a surface of an elastic solid has been carried out experimentally by analyzing the scattered ultrasonic waves. The inverse Born approximation (developed for flaws in bulk materials) was tested for the first time in the determination of the size, shape, and orientation of near-surface flaws. We have studied spherical solid inclusions at various depths below the surface. In addition we examined an approximately 2:1 prolate spheroidal inclusion which was located one major axis below the surface. The determination of the flaw's size, shape, and orientation in terms of an equivalent ellipsoid is realized by performing nonlinear least-squares iteration of the one-dimensional Born inversion results obtained at various scattering directions within a finite aperture. The reconstruction is in good agreement with the actual parameters of the flaw.
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    Journal of nondestructive evaluation 2 (1981), S. 43-49 
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    Keywords: Rayleigh wave dispersion ; higher mode generation ; cold-working ; rail ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The propagation of the Rayleigh surface wave is experimentally studied along the top surface of used railroad rail under conditions where ultrasonic pulses have carrier frequencies ranging from 0.4 to 3.0 MHz and approximately 10 µs duration. The generation of the first higher (M 21 or Sezawa) mode as well as the fundamental (M 11) mode and their dispersion properties are observed. These phenomena are attributable to the presence of the cold-worked surface layer caused by the wheel passage. It is shown that a theoretical model of a single layer overlying a half space, whose elastic constants are determined by a destructive method, yields results which agree with the dispersion curves obtained experimentally. On the basis of this one-layered model, an inversion method to estimate the layer thickness and its elastic constants is discussed.
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    Journal of nondestructive evaluation 2 (1981), S. 65-74 
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    Keywords: NDE ; cast iron ; vibration analysis ; modulus ; strength
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    Notes: Abstract An outline is given of the existing nondestructive vibration tests for cast irons and their drawbacks in terms of the dimensional accuracy of the components. A proposal is made for a new test based on the change of resonant frequency with vibration amplitude exhibited by cast irons. This proposal is evaluated using material data obtained from dynamic tests of a wide range of cast iron specimens. A test rig is described suitable for testing cylindrical cast bars, and typical results are presented in relation to material strength. There is also a brief description of preliminary testing on cast products.
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    Journal of nondestructive evaluation 2 (1981), S. 113-118 
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    Keywords: Capacitance ; dielectric constant ; voids ; cellular glasses ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Cellular (foamed) glasses are a prime candidate for certain structural applications, such as support of the silvered glass concentrator mirrors in solar thermal energy systems. An economical nondestructive measurement of strength-limiting defects in these materials would permit development of designs in which the material is subjected to a higher working stress level and maintains the same probability of failure. This would result in weight and cost savings for any application. A method of measuring the capacitance of well-defined regions of a specimen was investigated. Large voids were found to produce significant localized changes in the capacitance of the specimen. The feasibility of locating the large, strength limiting defects in this porous material has been demonstrated for one type of cellular glass. Means of producing an image from this type of data and an implementation scheme for on-line production measurements are discussed.
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  • 43
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    Journal of nondestructive evaluation 2 (1981), S. 139-146 
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    Keywords: Material defects ; 14 MeV neutrons ; pulse-shape analyzer system ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract A 14 MeV neutron generator and fast neutron spectrometer consisting of an organic scintillation detector and a pulse-shape analyzer are used for the nondestructive testing of materials. The 14.6 MeV peak (in the 12.7–15.7 MeV region) is observed to change as a function of both the kind of testing material and the defect condition inside the material. The number of neutron counts under the 14.6 MeV peak for lighter testing materials (e.g., plastic, brass, and aluminum) with a defect will increase, as compared to the number of neutron counts under the same peak region of an identical testing material but with no defect. For heavier testing materials (e.g., steel and lead), the inverse is observed. The method is feasible even with defects of size 0.25 cm3 (i.e., a cylindrical cavity 0.4 cm in diameter and 2.0 cm in length), although quantitative evaluation of the size of the defect as a function of the change of the number of neutron counts under the 14.6 MeV peak region is difficult due to instability of the 14 MeV neutron output.
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    Journal of nondestructive evaluation 2 (1981), S. 219-229 
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    Keywords: acoustics ; surface acoustic waves ; Rayleigh waves ; ceramics ; surface cracks ; residual stresses ; crack extension ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract An investigation of scattering from surface cracks has been conducted. In particular, the change in the reflection coefficient of a Rayleigh wave incident on a surface indentation crack has been measured as the sample is stressed to fracture. The acoustic measurements have been correlated with the stable crack extension that precedes final failure. The crack extension behavior of as-indented specimens was found to differ appreciably from that of annealed specimens. Cracks in the annealed samples exhibited partial crack tip closure, but little stable extension, whereas cracks in the as-indented samples displayed both crack closure and irreversible crack growth. This behavior has been rationalized by invoking concepts based upon the residual stresses created by indentation.
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    Journal of nondestructive evaluation 3 (1982), S. 9-17 
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    Keywords: ac potential difference ; probe characterization ; induction effect ; slots ; fatigue crack ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract In the ac field method of crack depth measurement by the Crack Microgauge, the area of the loop formed in the probe gives rise to an induced voltage, which can introduce errors into the depth measurement. In this paper, a method for measuring the probe area is given, and the quality of the probe is thereby characterized. The underlying theory was given previously, and it is applied here to the probe characterization problem. The probe area is determined by two voltage measurements taken on an artificial rectangular flaw machined in an arbitrary metal. By measurements on several such specimens with the same probe, it is confirmed that the area so obtained is a characteristic of the probe and is independent of the specimen material. Thereafter, measurements on various rectangular flaws with probes of different characteristic area were taken, and very good agreement between predicted and real depths was achieved. Both theory and experiments show that probe characterization is of particular importance when this method is used to measure surface crack depths in metals of low permeability such as aluminum.
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  • 46
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    Journal of nondestructive evaluation 3 (1982), S. 37-43 
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    Keywords: eddy current ; impedance ; lift-off ; flaw ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The problem of detection and characterization of a flaw in a conducting half-space using an eddy-current coil oriented parallel to the interface is examined. An expression is derived for a first order approximation for the change in complex impedance due to a flaw located within the conducting medium. The overall impedance is a function of the radius and lift-off distance of the test coil and the conductivity of the material. An analytical expression is derived for the change in impedance as a function of the electric fields at the position of the flaw. It is found to be an integral over the volume of the flaw of the electric fields found with and without the flaw being present. The limiting case of a degenerate point flaw may be examined in greater detail by allowing the field in the presence of the flaw to be approximated by the unperturbed field. For flaws small enough that the field does not vary much over its volume, the field may be even further approximated by using just the value of the field at the position of the centroid of the flaw. Plots are shown to illustrate the behavior of the change in impedance as a function of the radial range of the flaw and the depth of the flaw centroid, using previously derived expressions for the fields for the unflawed case.
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  • 47
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    Journal of nondestructive evaluation 3 (1982), S. 85-91 
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    Keywords: displacement-field ; acoustic radiation ; wave propagation ; absolute velocity ; EMAT ; mode selective ; nonresonant frequency response ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract A new electromagnetic acoustic transducer (EMAT) is developed that measures velocities and absolute surface displacements in-plane and plane-normal to the direction of wave propagation. This transducer is flexible in shape and design to conform with irregular surfaces and has unique characteristics of nonresonant frequency response. Tests show that the sensitivity of this EMAT can be increased by increasing the applied magnetic field. Because this transducer is mode selective and nonresonent, its response can be related uniquely to the wave motion. This property is highly desirable for research purposes, though generally not wanted in field monitoring situations.
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  • 48
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    Journal of nondestructive evaluation 3 (1982), S. 221-227 
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    Keywords: Radiography ; point-spread function ; corner location ; edge location ; unsharpness ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Two-dimensional imaging properties of locally isotropic and isoplanatic point spread functions are described in a general radiographic context. In particular, the radiographic response to a two-dimensional wedge-shaped object is shown to possess several properties which enable the identification of the wedge corner on the image, thereby alleviating the need for image enhancement techniques. Extensions to the location of flat-edged boundaries are also discussed. The potential dimensioning application of this analysis lies in the precise and objective location of points of interest on radiographs of more complex two-dimensional objects.
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    Journal of nondestructive evaluation 3 (1982), S. 229-239 
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    Keywords: Crack-opening displacement ; crack closure ; ultrasonics ; Kirchoff approximation ; NDE
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    Notes: Abstract The interaction between rough crack faces is modeled by nonlinear relations between the crack-face tractions and the crack-opening displacements. These relations account for crack closure and for the related resistance to crack-face sliding. The relations are used to investigate reflection and transmission of an incident pulse by an infinite flaw plane. The problem statement is reduced to a set of inhomogeneous nonlinear ordinary differential equations for the displacement discontinuities, [u] and [v], across the flaw plane. These equations have been solved numerically. The reflection and transmission of an incident pulse by a crack with interacting crack faces. Both incident longitudinal and transverse waves have been considered. The loss of specular reflection as compared to a perfect (traction-free) crack is exhibited by specific examples.
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    Journal of nondestructive evaluation 2 (1981), S. 51-55 
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    Keywords: Love wave dispersion ; surface layer ; work hardening ; rail ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract In this paper, experiments show Love wave generation along the top surface of used railroad rail, where the shear wave velocity has been slightly reduced by the cold-working of wheel passage for years. The rf pulses used in the experiments have about 10 µs duration and a relatively narrow frequency spectrum. The group velocity of the Love wave is found to have a strong dependence on the carrier frequency over the tested range of 0.45–3.1 MHz. Application of the seismological one-layered model to the experimental measurements yields an NDE technique for the elastic properties and the thickness of the cold-worked surface layer. The results are interpreted on the basis of a destructive observation by micro-Vickers hardness testing.
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    Journal of nondestructive evaluation 2 (1981), S. 75-84 
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    Keywords: digital signal processing ; microprocessor based system ; binary display ; ordered dither ; ultrasonic C-scan ; image magnification ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The use of a digital microprocessor based system for the acquisition of ultrasonic C-scan information is investigated. The C-scan information is displayed on a binary display device using electronic ordered dither techniques to represent gray levels. The digital system with a binary display increases system flexibility and yields better reproducibility and constant image quality independent of the display medium. Images may be stored on magnetic tape or disk for later retrieval and image processing. The techniques which are described allow for image magnification and a reduction in scan time by replacing the mechanical linkage between the scanner and the display with digital signals. A detailed comparison is made between two dither signals, and the advantages of each are discussed. The combination of digital signal processing and imaging techniques produces results which utilize the capabilities of ultrasonic inspection to the point where the transducer becomes the limiting factor.
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    Journal of nondestructive evaluation 2 (1981), S. 133-138 
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    Keywords: X-rays ; diffraction ; grain size determination ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract It is shown that the grain size of commercial materials can be estimated by x-rays with little or no sample preparation. The method is readily automated and can be employed on-line, for example, in a rolling mill.
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    Journal of nondestructive evaluation 2 (1981), S. 173-188 
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    Keywords: ultrasonics ; acoustic imaging ; computer image analysis ; failure prediction ; inhomogeneous materials ; accept/reject criteria ; NDE
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    Notes: Abstract A computerized technique is presented which has been developed for analyzing acoustic images of an inhomogeneous material. The acoustic imaging system is briefly described with emphasis on its adaptation to billets of graphite for atmospheric reentry body use. Ultrasonic images of such inhomogeneous material contain a nonuniform distribution of anomalies and few, if any, geometrically regular features which can be assessed to establish material integrity. Results obtained with our new algorithms show, for certain discriminants, a high degree of correlation between the numerical accept/reject values obtained from acoustic image processing and the time-to-failure of the graphitic material in simulated atmospheric reentry tests.
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    Journal of nondestructive evaluation 2 (1981), S. 209-217 
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    Keywords: honeycomb panels ; acoustic inspection ; leaky waves ; SH and Lamb waves ; line scan ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract This paper considers the properties of elastic waves guided by an aluminum plate bonded to a honeycomb core for application to rapid inspection of honeycomb panels. Current acoustic inspection techniques involve the transmission of a signal between a pair of small transducers located on opposite sides of the panel. Scanning the transducers in raster fashion results in a high resolution inspection of the panel, but is very time consuming. An alternative technique would simultaneously inspect all points along a line between two widely spaced transducers located on the same side of the panel. Scanning the pair once over the panel permits rapid inspection, although with decreased resolution. Studies presented here indicate that such a method of inspection is feasible and that the flexural mode is probably the most useful.
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    Journal of nondestructive evaluation 3 (1982), S. 1-8 
    ISSN: 1573-4862
    Keywords: acoustoelasticity ; transverse isotropy ; polycrystalline aggregate ; cubic crystal ; third-order elastic constants ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Acoustoelasticity is an ultrasonic technique which has been used for the determination of active and residual stresses in common structural materials. This paper examines the effect of texture on the acoustoelastic response in polycrystalline bodies. In particular materials which are transversely isotropic aggregates of cubic crystals are studied. The second- and third-order elastic constants of the polycrystal are derived from the elastic properties of the constituent crystals, and the crystalline orientation relative to the body's symmetry axis. The acoustoelastic relations between velocity and deformation are then presented for the aggregate. Finally, evaluation of the acoustoelastic response for several ideal textures using data for aluminum single crystals shows that the response is highly dependent on the texture.
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    Journal of nondestructive evaluation 3 (1982), S. 111-113 
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    Keywords: photoacoustic microscopy ; delamination ; layered structure ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract An adhesive layer, by which a piezoceramic foil is attached to an aluminum membrane for telephone application, is examined in a nondestructive way by thermal waves. The basic principles of the method are outlined, and its detecting power is demonstrated by evaluation of a well-defined delamination.
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  • 57
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    Journal of nondestructive evaluation 3 (1982), S. 147-154 
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    Keywords: finite element modeling ; eddy current ; absolute probe ; steam generator tubing ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Finite element analysis techniques are applied to the problem of predicting signals from an absolute eddy current probe in the tube sheet region of a PWR steam generator for the purpose of optimizing the probe coil geometry and determining the feasibility of using such a probe to characterize the condition of the tube and tube sheet crevice.
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  • 58
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    Keywords: Acoustic harmonic generation ; critical angle acoustics ; fatigue crack detection ; alloy microstructure ; load amplitude ; NDE
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    Notes: Abstract Alloy grain size is known to influence both the propagation path and the closure stress of surface fatigue cracks in many alloys. The general trend is for the path to be more tortuous and the closure stress to be larger, the larger the grain size. By use of Ti Al-4V and Al 7075-T6, the effects of grain size on the nondestructive detection of surface cracks which might arise from closure stress and path irregularity variables were evaluated. Titanium specimens were inspected using an acoustic harmonic generation technique, and it was discovered that the major source of harmonic signals was grain sized crystallographic cracks. Harmonic signals were larger during fatigue in an 8-µm compared to a 4-µm grain sized alloy, as there were more grain sized cracks in the large grain material. Crack closure was found to be extremely important in determining the reflected acoustic amplitude obtained in inspecting small (100–1000 µm) cracks in Al 7075-T6 using a critical angle technique. Average received amplitudes were an order of magnitude smaller for cracks at zero load than for those opened by a tensile stress. The scatter in the reflected amplitude was also large, apparently as the result of variations in the degree of the closure from crack to crack. For the 7075 material, the important effect of larger grain size was to increase the irregularity of the crack path, making the small cracks more visible acoustically at azimuthal angles not normal to the crack plane.
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  • 59
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    Journal of nondestructive evaluation 3 (1982), S. 137-145 
    ISSN: 1573-4862
    Keywords: jadeite authentication ; 14 MeV neutrons ; jades ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Fourteen MeV neutron activation of jades was used to test the authenticity of jades: to ascertain whether they are genuine jadeite and whether their greenish colour is genuine, both of which are important criteria for the high market value of jadeite. For given activation and measurement conditions of the jades, the gamma-ray spectrum was observed to change as a function of the type of the jades; the counting ratio of the photopeaks from the two most prominent constituent elements varies greatly as a function of the type of the jades. The ratio of counts for the Compton edges of the 1.779 MeV gamma-ray of28Al, from28Si(n,p)28Al reaction, and the 1.434 MeV gamma-ray of52V, from52Cr(n,p)52V reaction, varied as a function of the type of the jades and also as a function of the color. These results can be applied for a fast and nondestructive evaluation of jades.
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  • 60
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    Journal of nondestructive evaluation 1 (1980), S. 101-109 
    ISSN: 1573-4862
    Keywords: ultrasonic transducers ; NDE ; deconvolution ; signal processing
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract A major objective of quantitative nondestructive evaluation is to formulate defect detection, classification, and sizing algorithms that are insensitive to variations in transducer characteristics, material type, and defect depth. With the data used in this research, ultrasonic signals were found to vary significantly with changes in the transducer and only secondarily with changes in material and depth. It is shown that the method for minimizing signal variations due to transducer and material changes is to deconvolve the test signal with respect to the transducer response from a reference defect in a block of the same material. Since depth variation primarily affects signal amplitude and has minor impact on shape, detection, classification, and sizing, insensitivity to depth is achieved by avoiding the use of amplitude-dependent parameters. The notion of a “standard transducer” is introduced. Its mathematical properties and methods of realization are given. It is shown how the effects of variability from different test transducers can be removed by signal processing. When these procedures are applied to the test transducer, the effect is to cause it to resemble the standard transducer and, thereby, to place all ultrasonic waveforms on a common basis.
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  • 61
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    Journal of nondestructive evaluation 1 (1980), S. 137-148 
    ISSN: 1573-4862
    Keywords: composite material ; ultrasonic velocity ; ultrasonic attenuation ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The ultrasonic group velocity and attenuation were measured as a function of frequency for longitudinal and shear waves in the Hercules epoxy matrix (3501-6) and in the principal directions of the unidirectional Hercules graphite fiber epoxy composite (AS/3501-6). Tests were conducted in the frequency ranges 0.25–14 MHz and 0.5–3 MHz for longitudinal and shear wave modes, respectively. While the attenuation increased with frequency for all wave modes, the group velocity was independent of frequency for all wave modes. In studying the effects of transducer-specimen interface couplant and pressure, it was found that for each transducer, there exists a frequency-dependent “saturation pressure” corresponding to the maximum output amplitude of the signal.
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  • 62
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    Journal of nondestructive evaluation 1 (1980), S. 149-155 
    ISSN: 1573-4862
    Keywords: acoustic emission ; fracture ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The far-field characteristics of the emission from a theoretical model for the fracture of brittle inclusions are presented in detail. The model is a circular crack growing at constant speed from zero size until it attains a prescribed size. The far-field radiation pattern is the same as that of a simple combination of force doublets, and some qualitative similarities between force doublets and acoustic dipoles are noted. The initial shape of the far-field pulses due to the growing stage and the stopping is determined, but difficulties arise in accounting for the diffraction of a surface wave on the crack faces generated by the stopping of the crack.
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  • 63
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    Journal of nondestructive evaluation 1 (1980), S. 215-224 
    ISSN: 1573-4862
    Keywords: Acoustic emission ; slip ; microcracking ; deformations ; displacement field ; elastic waves ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The theory of elastic wave emission (i.e., acoustic emission; AE) from damage processes such as slip and microcracking is discussed. Analogous developments in the literature on earth-quake seismology and dynamic dislocation theory are noted and utilized. A general representation of the displacement field of an AE event is given in terms of the double-couple response to a distribution of “moment density tensor” in the source region. Results are specialized to a point source model and to a general far-field analysis of outgoing elastic waves, and conditions for validity of such representations and their low-frequency specializations are noted. Emitted wave fields are compared for tensile opening and slip events, and procedures which might enable the approximate determination of the size or area increase of tensile microcracks are discussed.
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  • 64
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    Journal of nondestructive evaluation 1 (1980), S. 157-181 
    ISSN: 1573-4862
    Keywords: EMAT ; radiation pattern ; transducer ; shear waves ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract A Green's function calculation of the far-field radiation patterns of EMATs is presented. The approach is based on (a) closed form expressions for the eddy current and static magnetic field distributions, established by the EMATs, which react to produce the driving Lorentz forces, and (b) a Green's function derived from the steepest descent approximation to the far-field response of an arbitrary surface point force on a half space. Numerical results are presented, illustrating the radiation patterns of the three common EMAT designs. Included are vertically polarized shear waves as radiated by both meander coil and periodic magnet EMATs and horizontally polarized shear waves as radiated by the latter.
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  • 65
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    Journal of nondestructive evaluation 1 (1980), S. 123-136 
    ISSN: 1573-4862
    Keywords: fatigue life ; acoustic surface waves ; NDE ; ultrasonics ; cracks
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract The present studies concentrated on predicting the remaining fatigue life for single fatigue cracks in the Paris regime of macrocrack propagation. Acoustic surface waves were used to interrogate the crack during cyclic fatigue. The inversion of the obtained scattering data provided crack depth and crack length as a function of the number of cycles applied in tension-tension fatigue. Auxiliary experiments were conducted to study the acoustic response of the crack to tensile and compressive loads, thought to open and close the crack. The technique may allow for new insights into the physics of the “crack closure” effect.
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  • 66
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    Journal of nondestructive evaluation 1 (1980), S. 201-206 
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    Keywords: fracture mechanics ; noncircular crack ; fatigue life ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract A transfer function that converts an irregularly shaped two-dimensional crack into an equivalent circular crack of equal propagation lifetime is proposed. The methodology extends the exact elliptical to circular crack transfer function to other noncircular cracks with geometries that slightly deviate from the elliptical. Results for rectangular, symmetric dumbbell, and asymmetric dumbbell cracks are presented.
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  • 67
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    Journal of nondestructive evaluation 1 (1980), S. 249-261 
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    Keywords: Ultrasonics ; cracks ; surface roughness ; diffraction ; scattering ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Ultrasonic measurements from rough cracks were carried out using both broad-band and narrow-band methods. An analysis is suggested to determine parameters of the crack quantitatively such as size, shape, rms surface roughness, and distribution function of the surface roughness. Ultrasonic measurements of the parameters compare very well with the actual parameters of the defect.
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  • 68
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    Journal of nondestructive evaluation 1 (1980), S. 287-293 
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    Keywords: Photoacoustic microscopy ; SPAM ; silicon nitride ; surface crack ; turbine vanes ; NDE
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    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Scanning photoacoustic microscopy (SPAM) was applied to silicon nitride turbine hardware for purposes of detecting fabrication defects. SPAM traces in selected areas of complex-shaped turbine stator vanes were obtained and indicate that surface-crack detection of the order of tens of microns is possible. Means were devised to allow for multiple scans in areas previously inaccessible to other nondestructive techniques capable of detecting defects in the 25- to 100-µm range. The results show that SPAM is applicable to reaction-bonded silicon nitride turbine components and capable of detecting small surface-related defects.
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  • 69
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    Cell & tissue research 206 (1980), S. 55-63 
    ISSN: 1432-0878
    Keywords: Phagocytosis ; Spermatozoa ; Ovum ; Fertilization ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary Spermatozoa with intact acrosomes, as well as those coming into contact with the ovum at a smaller angle, and morphologically abnormal spermatozoa reach the plasma membrane of the ovum via an extensively dissolved zone of the inner layer of the vitelline membrane. This zone is assumed to be formed by overlapping of two or more tunnels formed by spermatozoa that had previously come into contact with the ovum. When a spermatozoon comes into contact with the plasma membrane of the ovum, many cytoplasmic processes extend outwards and cover it. Thereafter, the plasma membranes of the processes fuse, thereby phagocytizing the spermatozoon. It is assumed that the phagocytized spermatozoa cannot undergo transformation into male pronuclei and that they degenerate soon after phagocytosis.
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    Cell & tissue research 206 (1980), S. 225-232 
    ISSN: 1432-0878
    Keywords: Compound eye ; Photoreceptor membrane ; Electron microscopy ; Calcium-induced changes ; Artefacts ; Diptera
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    Topics: Biology , Medicine
    Notes: Summary When the compound eyes of the fly Lucilia are fixed for electron microscopy with glutaraldehyde in common buffer solutions, artefactual whorls are liable to be formed from the photoreceptor microvilli. The whorls result from two factors: (i) a prolonged time interval prior to osmication, such as the “overnight” primary fixation or wash at 4° C commonly used in studies of compound eyes; (ii) as little as 1–2 mM Ca++ in the primary fixative and wash solutions. Osmication after short (1 h) glutaraldehyde fixation at 4° C, or omission of Ca++ and addition of 2 mM EGTA, prevent whorl-formation. In the tipulid fly Ptilogyna, similar artefacts are produced, but are confined to the distal zone of the microvilli that sheds during turnover.
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    Cell & tissue research 211 (1980), S. 83-93 
    ISSN: 1432-0878
    Keywords: Epithelium ; Cloaca ; Electron microscopy ; Hen
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    Topics: Biology , Medicine
    Notes: Summary The epithela of the three divisions (coprodaeum, urodaeum, proctodaeum) of the cloaca of the hen, and of the excretory ducts (colon, ureter, vagina) which join the divisions, are described using light microscopy, and scanning and transmission electron microscopy. Each region of the cloaca has its typical epithelium. Special attention is focussed in this study on the boundaries between the different epithelia. The coprodaeal epithelium does not differ considerably from that of the colon; a transitional zone is not visible. Distinct border zones, however, are observed between the other regions (ureter — urodaeum; vagina — urodaeum and proctodaeum; urodaeum-proctodaeum; proctodaeum — cutis). Although the vaginal opening is generally thought to lie in the urodaeum, our investigations show that at the vaginal opening into the cloaca the ciliated epithelium changes, on one border to a secretory epithelium characteristic of the urodaeum and on the other border to that characteristic of the proctodaeum. These observations are discussed in relation to functional aspects.
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    Cell & tissue research 211 (1980), S. 171-174 
    ISSN: 1432-0878
    Keywords: Pancreatic D cell ; Neural control ; Vagotomy ; Electron microscopy ; Fowl
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    Topics: Biology , Medicine
    Notes: Summary In an attempt to determine the neural control of pancreatic D cells, the pancreatic islets of the domestic fowl were examined electron microscopically from 1 to 28 days after abdominal vagotomy. Exocytotic release of many secretory granules from D cells occurred one day after vagotomy. Rough endoplasmic reticulum developed and formed an arrangement of concentric whorls in the cytoplasm of D cells after axotomy. The altered D cells were also characterized by the occurrence of many peculiar dense bodies in the apical cytoplasm at all time periods studied. These bodies varied in shape and size, containing several round vesicles. The D cells were extensively depleted of granules after the longer time periods following vagotomy. The present results provide new morphological evidence for the vagus-nerve control of D cells, which may regulate the activity of islet cells.
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  • 73
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    Cell & tissue research 211 (1980), S. 191-206 
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Transplants ; Vasopressin ; Median eminence ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary Blocks of anterior hypothalamus were transplanted from 19 day-old fetuses of Wistar/Lewis rats into the third ventricle of adult male Brattleboro rats. Physiological changes in graft recipients and in sham-operated animals were monitored daily. Twenty days after surgery, the graft recipients and shamoperated animals were killed and their brains examined by correlative scanning and transmission electron microscopy. Host animals that exhibited both decreased polydipsia and increased urine concentration were found to have viable grafts within the third ventricle. The observed physiological changes suggested that synthesis and release of vasopressin occurred in the transplanted neurons. Grafts were well vascularized by vessels arising from the host hypothalamus. Neurons, with perikarya ranging from 8 to 30 μm in diameter, glial cells, and neurites were located throughout the transplants. A neurohemal contact zone, similar to that normally seen in the median eminence, could not be demonstrated in the grafts. The absence of complete glial and ependymal barriers indicates a relatively close association between cells in the transplants and the cerebrospinal fluid. A large increase in supraependymal neurons and their processes, including an eruption of neurons through the floor of the third ventricle in one animal, was observed in graft recipients but not in shamoperated animals.
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  • 74
    ISSN: 1432-0878
    Keywords: Key words Prion protein (PrPc) ; Electron microscopy ; Secretory granules ; Membrane ; Extracerebral tissues ; Hamster ; Human
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    Notes: Abstract  In transmissible spongiform encephalopathies (TSE), such as scrapie in animals and Creutzfeldt-Jakob disease in humans, the central event is the conversion of a host-encoded amyloidogenic protein (PrPc) into an abnormal isoform (PrPsc) that accumulates as amyloid in TSE brain. PrPc is a membrane sialoglycoprotein synthesized in the central nervous system and elsewhere. We have examined the ultrastructural localization of PrPc in numerous hamster and some human extracerebral tissues, by means of a post-embedding electron-microscopic method combined with immunogold labeling. In stomach, intestine, lung, and kidney from hamsters, and in stomach, kidney, and spleen from humans, immunogold labeling specific for PrPc is observed on various cellular substructures related to secretory pathways: Golgi apparatus, secretory globules, and plasma membrane. In mucous epithelial cells of stomach and intestine, PrPc appears to be concentrated in secretory globules, suggesting a role for PrPc in the secretory function of the digestive tract. The secretory aspect of PrPc may be a key to understanding the physiopathological mechanisms underlying TSE.
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  • 75
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    Cell & tissue research 206 (1980), S. 303-318 
    ISSN: 1432-0878
    Keywords: Area postrema, rat ; Ependyma ; Cyst ; Circumventricular organs ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary Peculiar cells forming cysts were observed in the area postrema and sometimes also in the choroid plexus and the tela chorioidea near the area postrema, and were studied in detail by electron microscopy. The cytological features of the cyst cell and its junctional relationship to neighboring cells imply that cyst cells are derived from ependymal and choroid epithelial cells. The cyst cells usually contact directly the perivascular spaces of postremal, choroidal or pial capillaries, where the cytoplasm is often considerably attenuated. The cystic lumen is commonly filled with a flocculent material. The limiting membrane of the cystic lumen, which frequently bears cilia and microvilli, has the same thickness as the surface cell membrane. In many cases, the cyst is surrounded by the cytoplasm of a single cell. In some cases, however, two cells participate in the formation of the cyst, although one is only a slender process and joined by a zonula occludens with the main cyst cell. Horseradish peroxidase (HRP) injected into the cerebrospinal fluid (CSF) space failed to enter the cystic lumen. A possible significance of the cyst in relation to the CSF and blood circulation was considered.
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    Cell & tissue research 210 (1980), S. 447-459 
    ISSN: 1432-0878
    Keywords: Lipid ; Kidney tubules, proximal ; Autoradiography ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary Lipid metabolism in the cells of the renal proximal convoluted tubules (PCT) was investigated in healthy fowls and in fowls with the Fatty Liver and Kidney Syndrome (FLKS). The tissue was fixed at 10–25 min intervals after intravenous injection of 3H-oleic acid. The distribution of autoradiographic grains was analysed by the “circle method”. In normal cells most of the silver grains were associated with the cytoplasmic organelles. Lipid droplets and Golgi elements had the highest specific activity relative to the nuclear activity, which was little above background level. Lysosome-like bodies and mitochondria had lower values. In the cells of the FLKS-affected birds a large proportion of the grains was located over the lipid droplets, which are abundant in this condition. The specific activity of the cytoplasmic organelles was barely 2-fold higher than the nuclear activity. The results suggest that there is a diminished incorporation of esterified fatty acids by the organelles of these cells and that the excess is transferred to the lipid droplets. The identity of low electron density particles observed in the PCT cells of severely affected birds is discussed.
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    Cell & tissue research 211 (1980), S. 331-343 
    ISSN: 1432-0878
    Keywords: Gut hormones ; Endocrine cells ; Electron microscopy ; Immunocytochemistry ; Peptidergic innervation
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    Topics: Biology , Medicine
    Notes: Summary Histological, cytochemical and immunocytochemical methods were used in light and electron microscopical studies to demonstrate the presence of a neuroendocrine system in the gut of the urodele, Salamandra salamandra. Cytochemical stains capable of detecting peptide-producing endocrine cells demonstrate cells reacting with Masson's silver (argentaffin) method, Grimelius' argyrophil silver method, masked metachromasia method and the lead haematoxylin stain. Using antisera raised to a variety of mammalian gut peptides, cells containing bombesin-, gastrin-, somatostatin-, substance P- and glucagon-like immunoreactivity were identified; vasoactive intestinal polypeptide- and substance P-like immunoreactivities were found in nerve fibres in the submucous and myenteric plexus. No immunoreactivity was detected for motilin, gastric inhibitory polypeptide, cholecystokinin or secretin. The ultrastructure of the immunoreactive cells and nerves was revealed by the semithin/thin method. All the cells identified contained numerous electrondense secretory granules, which varied in their chracteristic morphological structure from one cell type to another. The evidence collected in this study indicates that a complex neuroendocrine system regulating gut function is present in this amphibian and may have developed prior to the emergence of the phylum.
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    Cell & tissue research 293 (1998), S. 1-22 
    ISSN: 1432-0878
    Keywords: Key words Piecemeal degranulation ; Histamine ; Charcot-Leyden crystal protein ; Anaphylactic degranulation ; Electron microscopy
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    Notes: Abstract  The evidence for vesicular transport as a mechanism for secretion by human basophils is reviewed. Initially, direct electron-microscopic inspection of experimentally produced and sequentially biopsied contact allergy skin lesions revealed a unique form of secretion termed piecemeal degranulation, characterized by the slow emptying of secretory granule contents (with retention of empty containers) in the absence of extrusion of entire granules. Budding of small vesicles to/from secretory granules was observed, and cytoplasmic vesicles were abundant. A generalized degranulation model was proposed to unify classical regulated secretion and this new form of secretion. Investigation of the mechanism(s) of secretion from human basophils required the development of numerous tools and resources. Chief among these were: (a) isolation and purification of circulating basophils; (b) identification of specific growth factors to increase the supply of this rare granulocyte; (c) understanding of secretogogue mechanisms and reliable analyses of secreted basophil products; and (d) development of ultrastructural preparations allowing imaging of small vesicles and quantifiable small electron-dense tags for granule materials in small vesicles. Applications of these tools to well-defined models of basophil secretion have established a role for vesicles as a mechanism for effecting secretion of histamine and the Charcot-Leyden crystal protein from activated human basophils.
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  • 79
    ISSN: 1432-0878
    Keywords: Pancreatic islets ; Adrenergic innervation ; Insulin secretion ; Chemical sympathectomy ; Adrenalectomy ; Fluorescence histochemistry ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Morphological changes in the adrenergic innervation of pancreatic islets after chemical sympathectomy by use of 6-hydroxydopamine and the influence of the sympatho-adrenal system on insulin secretion were investigated in the mouse and rat. Fluorescence histochemistry revealed a clear-cut reduction in the number of adrenergic nerve fibers in the pancreatic islets 2 days after administration of 6-hydroxydopamine; the reduction was more pronounced in the rat than in the mouse. In the rat, a partial regeneration was seen after 6 weeks. In the pancreas of the mouse, after administration of 6-hydroxydopamine, a severe damage of unmyelinated nerve fibers was revealed electron microscopically. However, no ultrastructural or immunohistochemical alterations could be demonstrated in the endocrine cells of the islets. 6-Hydroxydopamine induced a depression of basal plasma insulin concentrations in mice and an elevation in rats. Adrenalectomy depressed basal plasma insulin levels in mice. The α-adrenoceptor antagonist phentolamine enhanced insulin secretion in normal mice. The secretory response of insulin to phentolamine was diminished by chemical sympathectomy and almost abolished by adrenalectomy or the combination of chemical sympathectomy and adrenalectomy. Thus, the effect of phentolamine is probably mediated by liberated catecholamines. It is concluded that basal insulin secretion is partially regulated by the sympatho-adrenal system and that species differences exist in this respect. In addition, the results suggest that endogenous catecholamines have the ability to promote insulin secretion.
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    Cell & tissue research 216 (1981), S. 471-479 
    ISSN: 1432-0878
    Keywords: Ovulation ; Perfusion ; Graafian follicle (Rabbit) ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the present investigation the ultrastructure of isolated rabbit ovaries, perfused with different media for various time periods, was studied. The steroid hormone production by the perfused ovary was also determined. Perfusion with Medium 199 results in prominent interstitial ovarian oedema which increases with perfusion time. Even after the addition of 6–10 % Dextran T40, oedema appears in the interstitial tissue of the ovary. Perfusion solutions with osmotically active colloid particles of large molecular size (Dextran T70; average molecular weight 70,000 and bovine serum albumin), cause less distortion in the ovarian structure, and ultrastructurally the ovarian tissues appear essentially the same as in the control ovaries. The results indicate that the perfused rabbit ovary, under strictly controlled conditions, can be used as an experimental model for studies of various aspects of ovarian function, including follicular rupture.
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  • 81
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    Cell & tissue research 210 (1980), S. 269-282 
    ISSN: 1432-0878
    Keywords: Monoaminergic neurons ; Retina ; Amacrine cells ; Neurotoxins ; Mudpuppy, Necturus maculosus ; Neurotransmitters ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The mudpuppy retina was investigated with the histofluorescence method of Falck and Hillarp in normal animals and in animals injected intraocularly with α-methylnoradrenaline, 5,6-dihydroxytryptamine, or a combination of the two drugs. Catecholaminergic amacrine cells were found to form a thin layer of terminals at the border between the inner nuclear and the inner plexiform layers. Catecholaminergic interplexiform cells were not found. Indoleamine-accumulating amacrine cells were also observed. They are fifteen to twenty times more numerous than the catecholaminergic cells, and their terminals occur diffusely throughout the inner plexiform layer. In a number of eyes the majority of the indoleamine-accumulating terminals were eliminated with intraocular injections of the neurotoxin, 5,7-dihydroxytryptamine, but the reproducibility of this effect was not consistent. Intravitreal injections of 5,6-dihydroxytryptamine were used to label both types of neurons for electron microscopy. They were found to make conventional type synapses on amacrine cells and, less frequently, on bipolar cells.
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  • 82
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    Cell & tissue research 210 (1980), S. 353-357 
    ISSN: 1432-0878
    Keywords: Merkel cells (reptiles) ; Epidermis ; Lizard ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Examination of the labial epidermis of the lizard Lacerta sicula revealed cells displaying all features of Merkel cells. These cells are located in the stratum basale of epidermal pegs and are arranged in clusters.
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  • 83
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    Cell & tissue research 211 (1980), S. 293-301 
    ISSN: 1432-0878
    Keywords: Paneth cell ; Hibernation ; Intestine ; Hypothermia ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of Paneth cells from jejuno-ileal segments of the small intestine of the ground squirrel, S. lateralis, was examined under normal euthermic conditions and during the profoundly depressed metabolic conditions of natural hibernation. Paneth cells obtained from hibernating animals gave evidence of markedly reduced activity when compared to Paneth cells from euthermic animals. In hibernating animals, the nuclei were smaller, with less prominent nucleoli and with an increased proportion of heterochromatin. In hibernating animals, the rough endoplasmic reticulum was fragmentary and poorly organized, in contrast to the typical arrangement of concentric lamellae seen in euthermic animals. Although the total number of ribosomes was decreased in hibernating animals, there were proportionally more free ribosomes than in euthermic animals. Paneth cells from hibernating animals also contained a greater number of apical secretory granules which were smaller and more variable in electron density than granules from control animals. These ultrastructural features indicate that during hibernation the Paneth cell is relatively quiescent.
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  • 84
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    Cell & tissue research 216 (1981), S. 205-214 
    ISSN: 1432-0878
    Keywords: Compound eyes ; Insects ; Crustaceans ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The basement membrane of the compound eye of four insect species and three crustacean species was investigated employing electron microscopy. The basement membrane consists of an extracellular (basal lamina) and a cellular portion, the latter being composed of the flattened terminal extensions of cone cells and accessory pigment cells in insects and distal pigment cells in crustaceans. Other cells can also contribute to the basement membrane. It is thus a complex structure in all well-developed compound eyes. The cellular contributions vary in different species and were found to correlate to specific taxonomic units.
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  • 85
    ISSN: 1432-0878
    Keywords: Gastrin ; CCK ; Median eminence ; Electron microscopy ; Xenopus laevis (Amphibia, Anura)
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    Notes: Summary By use of an anti-gastrin serum and colloidal gold- or ferritin-labelled sheep anti-rabbit γ-globulins, nerve fibres and nerve terminals containing a gastrin-like substance were characterized at the ultrastructural level in the median eminence of Xenopus laevis. These immunoreactive fibres contain neurosecretory granules displaying medium to high electron density and a mean diameter of 75 nm. Labelling intensity varies from granule to granule. This is the first demonstration at the ultrastructural level of the precise location of a gastrin-like hormone in the median eminence of a vertebrate.
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  • 86
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    Cell & tissue research 217 (1981), S. 211-223 
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Pars intermedia ; Electron microscopy ; Sheep
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light microscopy of coronal sections of the sheep pars intermedia revealed a compact, incompletely lobulated V-shaped region about 15–20 cells thick, situated between the pars distalis and the pars nervosa. A prominent hypophysial cleft and follicles containing a colloid-like substance were seen. Using electron microscopy, five cell types could be distinguished: pars intermedia glandular cells, pars distalis-like glandular cells, interstitial cells, follicular cells and cleft lining cells. The polyhedral to pear-shaped pars intermedia glandular cells predominated. They contained dense-cored, membrane-bound granules near the Golgi complex, and larger, irregular vesicles with finely granular contents of varying electron density throughout the remaining cytoplasm; exocytotic release of granules was occasionally observed. Smaller numbers of cells resembling those seen in the pars distalis were scattered throughout the pars intermedia. Interstitial cells usually possessed elongated cytoplasmic processes which extended between the glandular cells, and were characterized by deeply indented nuclei, elaborate junctional complexes and an absence of cytoplasmic granules. Cells lining the follicles resembled the interstitial cells. The major cells bordering the hypophysial cleft were triangular in section and bore irregular microvilli on their free surface. The pars intermedia appeared to be less vascular than the remainder of the hypophysis and only occasional fenestrated capillaries were seen. Nerve profiles were rare.
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  • 87
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    Cell & tissue research 207 (1980), S. 511-517 
    ISSN: 1432-0878
    Keywords: Female hamster ; Harderian gland ; Testosterone ; Tubular clusters ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Distinct differences occur in the pigmentation and ultrastructural features of the Harderian glands in male and female hamsters. The results of a study on the effect of testosterone on the fine structure of the female Harderian glands are presented here. Glands from three groups of hamsters were examined at intervals up to 49 days: (1) testosterone injected, receiving 2mg testosterone propionate in 0.1 ml sesame oil per day; (2) sham-injected, receiving 0.1 ml sesame oil per day; (3) untreated controls. Testosterone injections caused a reduction in the number of dark-brown pigment granules in the acinar cells starting on the 6th day, whereas clusters of tubules, typical of adult male glands, appeared on the 4th day and increased in number thereafter. Lamellar structures, normally present in the female gland, decreased in testosterone treated specimens. These changes reversed after cessation of testosterone treatment. It is concluded that exogenous testosterone administered to female hamsters modifies the pigmentation and ultrastructure of their Harderian glands towards the male type and that this is a reversable phenomenon. There also appears to be an inverse relationship between the presence of tubular clusters in the acinar cells, and the degree of pigmentation.
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  • 88
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    Cell & tissue research 208 (1980), S. 171-181 
    ISSN: 1432-0878
    Keywords: Microtubules ; Dendritic spine apparatus ; Synapse ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using techniques for enhanced microtubular preservation, including albumin pretreatment (Gray, 1975), occipital cortex of rats was studied electron microscopically at various ages of development. A close structural relationship was seen between microtubules, sacs of SER and the postsynaptic “thickening” in primordial spines and with the dense “plate” material of spine apparatuses. Stereoscopic preparations in addition show a more complicated substructure than previously described for the “plate”. Microtubules may contribute to the formation of the “plate” of the spine apparatus which in turn is associated with the postsynaptic “thickening” of the mature spine. Possible functional correlates are discussed.
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  • 89
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    Cell & tissue research 209 (1980), S. 131-135 
    ISSN: 1432-0878
    Keywords: Skeletal muscle ; Bupivacaine ; Degeneration ; Satellite cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rat lumbrical muscles were incubated in a concentration of 10-2 M bupivacaine for 5 or 15 min and examined after further incubation in the absence of the drug for periods totalling 1, 2, and 3h. Electron microscopy showed that muscle fibers and their component organelles and myonuclei underwent a series of irreversible degenerative changes. However, satellite cells retained their normal morphology under similar conditions. It is concluded that satellite cells are responsible for the rapid regeneration of muscles that follows degeneration induced by bupivacaine. The role of satellite cells in muscle regeneration is discussed.
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  • 90
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    Cell & tissue research 215 (1981), S. 643-649 
    ISSN: 1432-0878
    Keywords: T-lymphocytes ; Blast cells ; Autoradiography ; Electron microscopy ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary As a companion to an earlier study, the morphometric attributes of stimulated (blast-derived) lymphocytes in mouse axillary lymph nodes have been established using stereological and autoradiographic methods. Blast transformation was induced in vivo with dinitrochlorobenzene (DNCB) and stimulated cells were labelled with tritiated thymidine. Random samples of cells were taken for light and electron microscopic morphometry. In comparison to the unstimulated lymphocyte, the stimulated cell increased in size and possessed a greater plasma membrane surface area. Increase in cell size was the result of increases in the volumes of all measured subcellular compartments both in the nucleus and the cytoplasm. Heterogeneity of the stimulated cell population precludes firm conclusions regarding the significance of all these ultrastructural changes, though alterations in cell surface are discussed in the context of known biochemical differences accompanying blastogenesis.
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  • 91
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    Cell & tissue research 207 (1980), S. 135-142 
    ISSN: 1432-0878
    Keywords: Caudal neurosecretory system ; Poeciliidae ; Electron microscopy ; Salinity changes
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    Topics: Biology , Medicine
    Notes: Summary The urophysis or neurohemal contact site of the caudal neurosecretory system of Mollienesia sphenops, the black molly, was studied in animals adapted to an artificial seawater environment. This species of fish was chosen for these studies because of its known ability to osmoregulate and its adaptability to the laboratory aquarium. The urophysis of freshwater acclimated mollys contained an abundance of neurosecretory granules. However, in fish subjected to a seawater environment for one week the number of neurosecretory granules was significantly decreased. In addition, there was an increase in blood cell infiltration of the urophysis.
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  • 92
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus ; Sympathetic innervation ; Renin-angiotensin system ; Electron microscopy ; Fluorescence microscopy ; Tupaia belangeri ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary It has previously been reported that the primitive primate Tupaia belangeri develops a renal failure when exposed to psychosocial stress. In order to learn if this high susceptibility to stress of the Tupaia kidney can be correlated with morphological and functional parameters of the Juxtaglomerular apparatus (JGA) and the renin-angiotensin system, comparative experiments were performed on Tupaia and rat. Our results reveal an outstandingly high potency of the JGA and the renin-angiotensin system in Tupaia as evident from the following findings: The Tupaia JGA contains a great number of epithelioid cells abounding in renin granules (electron microscopy). The renin content of the Tupaia kidney is considerably higher than in the rat (radio-immunoassay). The sympathetic innervation of the kidney and especially of the JGA is abundant in Tupaia (fluorescence and electron microscopy). Catecholamine contents of the kidney and other organs are significantly higher in Tupaia than in rats (spectrophotofluorometry). Our results support the previously developed concept of a potent intrarenal neuroendocrine interaction at the JGA level favouring, under certain conditions of social stress, the development of acute renal failure in Tupaia belangeri.
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  • 93
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    Cell & tissue research 212 (1980), S. 465-474 
    ISSN: 1432-0878
    Keywords: Postcapillary venules ; Calf ; Lymph nodes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of the postcapillary “high endothelial venules” (HEV) of lymph nodes in calves was studied light and electron microscopically. These venules were detected light microscopically in the paracortical zone by their high cuboidal endothelium, a distinct basal lamina, the migration of lymphocytes through the vessel wall, and the dominance of lymphoid cells in the lumen, often completely obliterating the latter. Ultrastructurally, the endothelial cells (HEC) of the HEV were characterized by a prominent Golgi complex including many small vesicles, a few lysosome-like bodies, and a specific association between mitochondria and rough endoplasmic reticulum (MER). The HEC are connected by desmosomes, whereas the intimate contact points between migrating lymphocytes and endothelial cells could not be classified according to the well-defined junctional types. Lymphocyte migration occurred predominantly intercellularly, i.e., between endothelial cells. Although the overall appearance of the described vessel type in bovines bears resemblance to HEV in other investigated species, several differences occur that most probably are related to species variation.
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  • 94
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    Cell & tissue research 216 (1981), S. 557-568 
    ISSN: 1432-0878
    Keywords: Electron microscopy ; Neuroglia ; Silver impregnation ; Brain ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The silver-impregnation procedure of Tsujiyama is suitable for demonstration of all three classical types of neuroglial cells; in the present study it was used for electron microscopic identification of neuroglial cells in the brain of the cat. The aim of the present study was 1) to determine impregnated structural correlates of neuroglial cells at the light- and electron-microscopic levels, and 2) to determine whether the method of Tsujiyama is applicable for the electron microscopic identification of the single types of neuroglial cells. Silver deposits were observed over the cytoplasm and processes of astrocytes where numerous glial filaments were present. Oligodendrocytes and microglial cells may be precisely differentiated by use of Tsujiyama's silver impregnation method at the electron microscopic level due to the pattern of silver-deposition in these two basic types of cells. This silver-impregnation method combined with electron microscopy is thus suitable for a precise identification of neuroglial cells; the technique may prove to be very helpful in identification of such categories of neuroglial cells that encompass also the images of cells which cannot be classified by use of the standard methods.
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  • 95
    ISSN: 1432-0878
    Keywords: Key words Dendritic cells ; Maturation ; Intrahepatic translocation ; Immunohistochemistry ; Electron microscopy ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Specific populations of hepatic sinusoidal cells were stained with monoclonal antibodies that recognize monocytes/macrophages (ED1), tissue macrophages (Kupffer cells) (ED2), MHC class II (Ia) antigen (MRC OX6), and dendritic cells/γ,δ T-cells (MRC OX62) and analyzed by light and electron microscopy. The majority of ED1+ and/or ED2+ cells were localized to the hepatic parenchyma, whereas OX6+ and/or OX62+ cells were more densely distributed within Glisson’s sheath than in the hepatic parenchyma. Double-immunoperoxidase staining of normal liver for ED1, ED2, and OX6 identified dendritic cells (DC) of two different phenotypes, ED1+ED2–OX6+ and ED1–ED2–OX6+. DC can be classified into three different types based on ultrastructural characteristics. The first type (type I) is characterized by one or more long cytoplasmic processes and a well-developed lysosomal system. The second type (type II) has an inconspicuous lysosomal system, abundant hyaloplasm, and characteristic short cytoplasmic processes. The third type (type I–II) has cytologic features intermediate between those of type I and type II DC. At the electron-microscopic level, these three cell types are found in the sinusoidal lumen, whereas the majority of type II DC are located in the space of Disse and Glisson’s sheath. Furthermore, some OX6-labeled elongated DC appeared to traverse the lumen of sinusoids through endothelial pores to enter the space of Disse. One hour after intravenous injection of latex particles (0.81 μm in diameter), numerous latex-laden dendritic cells (ED1+OX6+, type I and type I–II) were detected in the lumen of hepatic sinusoids, but not in the space of Disse or Glisson’s sheath. These findings suggest that normal rat liver contains resident dendritic cells which downregulate phagocytic activity and mature into potent accessory cells during migration from the portal vein toward the central vein. These DC then traverse the sinusoidal lumen to the hepatic lymph system via the space of Disse.
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  • 96
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    Parasitology research 62 (1980), S. 31-38 
    ISSN: 1432-1955
    Keywords: Haemohormidium cotti ; Light microscopy ; Electron microscopy ; Taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Haemohormidium cotti, an organism of uncertain status, is redescribed from one of its type hosts, the marine fish,Cottus bubalis. The parasite occurs as irregularly round, oval, or elongate, intraerythrocytic bodies within blood and tissue preparations. Electron micrographs show features which suggest thatH. cotti may, as previously suspected, belong with the Piroplasmasida but no firm evidence of this is established. Possible division stages are identified in both light and electron micrographs. The taxonomic position ofHaemohormidium is discussed.
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  • 97
    ISSN: 1432-1955
    Keywords: Babesia microti ; Electron microscopy ; Ticks ; Ixodes ricinus
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  • 98
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    Cell & tissue research 220 (1981), S. 153-161 
    ISSN: 1432-0878
    Keywords: Cytoplasmic transport ; Insect ovary ; Autoradiography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ovarioles of Coccinella and Tenebrio are shown to be telotrophic — a characteristic normally associated with hemipterans rather than coleopterans. They possess an anterior region of trophic cells and a chain of oocytes. The trophic cells are connected with the latter by a series of nutritive tubes, and autoradiography has shown that RNA is transported along the tubes to the oocytes. However, the system in these beetles differs markedly from that of hemipterans in that the nutritive tubes do not contain an extensive complement of aligned microtubules. The significance of this to both the mechanism and the selectivity of transport is discussed.
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  • 99
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    Cell & tissue research 220 (1981), S. 201-212 
    ISSN: 1432-0878
    Keywords: Atretic oocyte ; Perca fluviatilis ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The regression of atretic oocytes in Perca fluviatilis was studied by histochemical, light and electron microscopic methods. The course of regression can be divided into three stages, the first two comprising the dissolution of the atretic oocyte and its phagocytosis by the granulosa cells of the follicular epithelium, and the third stage consisting of the dissolution of the granulosa cells themselves. The ultrastructure in all three stages shows only features related to phagocytosis and lysosome formation. In particular, there is no agranular endoplasmic reticulum formed within the phagocytically active granulosa cells, nor is there any 3β-hydroxysteroid dehydrogenase activity (3β-HSD). Large yellow-orange pigments, formed during the third stage of regression, are ascribed to a relative deficiency of lysosomes in lipid digestion, and do not result from a preceeding steroid-synthesising phase as in mammalian corpora lutea. Thus, the atretic oocyte of P. fluviatilis is considered not to give rise to a corpus luteum formation with endocrine function, but merely represents a degenerative structure.
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    Cell & tissue research 226 (1982), S. 589-608 
    ISSN: 1432-0878
    Keywords: Kidney (frog) ; Glomerulus ; Nephron ; Tight junctions ; Freeze-fracturing ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By the use of thin sections and freeze-fracture replicas the glomerular and tubular structures of the kidney of the frog (Rana esculenta) were studied with special reference to intercellular junctions. In the glomerulus the filtration barrier is of very variable thickness, and frequent tight and gap junctional contacts occur between podocyte processes. Although structurally less elaborate, the proximal tubule resembles its mammalian counterpart. In the initial part the tight junctions are relatively shallow but become very broad in the mid and distal portions of the proximal tubule. The proximal tubular cells are extensively linked by gap junctions. In some animals the shapes of the cells in the proximal and distal portions of the proximal tubule were markedly different. The distal tubule consists of two segments which differ mainly in the pattern of interdigitations and the structure of the zonulae occludentes. Similarities with the tight junctional morphology of the mammalian distal tubule are striking. In the first part of the distal tubule (diluting segment) a narrow band of parallel tight junctions is found closely resembling that found in the mammalian straight distal tubule; in the more distal part of the distal tubule, however, a broad band of anastomosing tight junctional strands exists, like the zonula occludens of the mammalian convoluted distal tubule. The connecting tubule displays cellular dimorphism: its wall contains a mixture of light and dark (flask) cells. The luminal and basolateral membranes of the flask cells are covered with numerous rod-shaped particles. The tight junctions of the connecting tubule are broad and increase in depth and number of strands along its length; they are typical of a very tight epithelium. In spite of several dissimilarities with phylogenetically younger kidneys our findings suggest that many structural principles of the mammalian kidney are also represented in the kidneys of amphibians. The structural-functional relationships are discussed.
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